AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar ZhongmuNo.1 / MsG0380017382.01


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsG0380017382.01.T01 MTR_3g106310 100.000 51 0 0 1 51 1 51 3.50e-32 105
MsG0380017382.01.T01 MTR_1g013070 100.000 51 0 0 1 51 1 51 3.50e-32 105
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsG0380017382.01.T01 AT4G31985 90.196 51 5 0 1 51 1 51 1.88e-29 98.2
MsG0380017382.01.T01 AT3G02190 88.235 51 6 0 1 51 1 51 4.41e-28 94.7
MsG0380017382.01.T01 AT2G25210 90.909 44 4 0 8 51 1 44 6.59e-24 84.0

Find 32 sgRNAs with CRISPR-Local

Find 47 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
AACTTATTGTAGTTAATTAA+AGG 0.251556 3:+95618359 MsG0380017382.01.T01:three_prime_UTR
ATTTAAGACAATCGATGTTT+TGG 0.282559 3:+95618280 MsG0380017382.01.T01:three_prime_UTR
GTGCAGCCGTCGAGCATTTC+CGG 0.303652 3:+95617838 MsG0380017382.01.T01:five_prime_UTR
TTAGATTAGGGCTTCGTGTT+AGG 0.305458 3:+95617795 None:intergenic
AAGATTCTGAAATTAGATTA+GGG 0.315313 3:+95617783 None:intergenic
AAAGATTCTGAAATTAGATT+AGG 0.315654 3:+95617782 None:intergenic
ATTCGTAATCAAGAAGAAAT+TGG 0.321220 3:+95617980 MsG0380017382.01.T01:CDS
CCGGAGGCAAGGATCGAAAA+TGG 0.389081 3:+95617857 MsG0380017382.01.T01:exon
GGCATTGTAATGTTATACTT+TGG 0.392128 3:+95618301 MsG0380017382.01.T01:three_prime_UTR
AGAATAGACCCATCCCTTAC+TGG 0.418157 3:+95618018 MsG0380017382.01.T01:CDS
TAGATTAGGGCTTCGTGTTA+GGG 0.422723 3:+95617796 None:intergenic
GGGTTTACAAGAAGCATTCT+CGG 0.431321 3:+95617816 MsG0380017382.01.T01:five_prime_UTR
TCCCTTACTGGATCCGTATG+AGG 0.452674 3:+95618030 MsG0380017382.01.T01:CDS
CACTGGCGCCGTACCAAGCT+CGG 0.478359 3:+95618186 MsG0380017382.01.T01:CDS
AACCCTAATAGTTCAAGAAA+AGG 0.479806 3:-95618240 None:intergenic
ACAATGCTAAGCGCCGTCAC+TGG 0.497185 3:+95618169 MsG0380017382.01.T01:CDS
CTCAAAATCCGAGCTTGGTA+CGG 0.513650 3:-95618194 None:intergenic
GCTTGGTACGGCGCCAGTGA+CGG 0.518206 3:-95618182 None:intergenic
TGAGGACTGACAACACCATC+AGG 0.522876 3:+95618048 MsG0380017382.01.T01:CDS
GTCCTCATACGGATCCAGTA+AGG 0.528387 3:-95618032 None:intergenic
GGACAAAACGCGGCAAAGCA+AGG 0.533748 3:-95618219 None:intergenic
GTCGAGCATTTCCGGAGGCA+AGG 0.547294 3:+95617846 MsG0380017382.01.T01:five_prime_UTR
TGGTGTTGTCAGTCCTCATA+CGG 0.570137 3:-95618043 None:intergenic
AGGACCTCAAAATCCGAGCT+TGG 0.583429 3:-95618199 None:intergenic
ATACGGATCCAGTAAGGGAT+GGG 0.586639 3:-95618026 None:intergenic
CATACGGATCCAGTAAGGGA+TGG 0.594687 3:-95618027 None:intergenic
AGAAATTGGCGAAGAAGATG+AGG 0.597939 3:+95617994 MsG0380017382.01.T01:CDS
ATTACGAATGTCTTGTGTGA+CGG 0.598814 3:-95617966 None:intergenic
TCCTCATACGGATCCAGTAA+GGG 0.602358 3:-95618031 None:intergenic
TTGCCTCCGGAAATGCTCGA+CGG 0.662148 3:-95617844 None:intergenic
TTCAAGAAAAGGACAAAACG+CGG 0.690502 3:-95618229 None:intergenic
CAGCCGTCGAGCATTTCCGG+AGG 0.690561 3:+95617841 MsG0380017382.01.T01:five_prime_UTR

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! AAATCCGAATTAATTAAAAA+AGG - Chr3:95618391-95618410 None:intergenic 15.0%
!! AACTTATTGTAGTTAATTAA+AGG + Chr3:95618359-95618378 MsG0380017382.01.T01:three_prime_UTR 15.0%
!!! TTTTTATTTTTTCCTCTTTT+AGG + Chr3:95618145-95618164 MsG0380017382.01.T01:intron 15.0%
!!! GACACCTTTTTTAATTAATT+CGG + Chr3:95618384-95618403 MsG0380017382.01.T01:three_prime_UTR 20.0%
!!! TATACATATTTAGTTTTGCT+GGG + Chr3:95618438-95618457 MsG0380017382.01.T01:three_prime_UTR 20.0%
!!! TATATAAGTAAGAACTTTTG+CGG + Chr3:95618327-95618346 MsG0380017382.01.T01:three_prime_UTR 20.0%
!!! TTATACATATTTAGTTTTGC+TGG + Chr3:95618437-95618456 MsG0380017382.01.T01:three_prime_UTR 20.0%
! ATTCGTAATCAAGAAGAAAT+TGG + Chr3:95617980-95617999 MsG0380017382.01.T01:CDS 25.0%
!!! ATTTAAGACAATCGATGTTT+TGG + Chr3:95618280-95618299 MsG0380017382.01.T01:three_prime_UTR 25.0%
AACCCTAATAGTTCAAGAAA+AGG - Chr3:95618243-95618262 None:intergenic 30.0%
GGCATTGTAATGTTATACTT+TGG + Chr3:95618301-95618320 MsG0380017382.01.T01:three_prime_UTR 30.0%
! GTCCTTTTCTTGAACTATTA+GGG + Chr3:95618238-95618257 MsG0380017382.01.T01:three_prime_UTR 30.0%
! TGTCCTTTTCTTGAACTATT+AGG + Chr3:95618237-95618256 MsG0380017382.01.T01:three_prime_UTR 30.0%
AAATGCGAAGAAAGAGTGAA+AGG - Chr3:95618083-95618102 None:intergenic 35.0%
AATGCGAAGAAAGAGTGAAA+GGG - Chr3:95618082-95618101 None:intergenic 35.0%
ATTACGAATGTCTTGTGTGA+CGG - Chr3:95617969-95617988 None:intergenic 35.0%
CTTAGCATTGTACCTAAAAG+AGG - Chr3:95618160-95618179 None:intergenic 35.0%
TTCAAGAAAAGGACAAAACG+CGG - Chr3:95618232-95618251 None:intergenic 35.0%
AGAAATTGGCGAAGAAGATG+AGG + Chr3:95617994-95618013 MsG0380017382.01.T01:CDS 40.0%
AGCGTCTCTTCAATCGTATT+AGG + Chr3:95617892-95617911 MsG0380017382.01.T01:intron 40.0%
GAAAGGGAAAGAATACCTGA+TGG - Chr3:95618066-95618085 None:intergenic 40.0%
GCGTCTCTTCAATCGTATTA+GGG + Chr3:95617893-95617912 MsG0380017382.01.T01:intron 40.0%
! AAGAACTTTTGCGGTGTTTG+AGG + Chr3:95618336-95618355 MsG0380017382.01.T01:three_prime_UTR 40.0%
!! GATTGAAGAGACGCTGATTT+GGG - Chr3:95617887-95617906 None:intergenic 40.0%
!! GGGTTTACAAGAAGCATTCT+CGG + Chr3:95617816-95617835 MsG0380017382.01.T01:five_prime_UTR 40.0%
AGAATAGACCCATCCCTTAC+TGG + Chr3:95618018-95618037 MsG0380017382.01.T01:CDS 45.0%
ATACGGATCCAGTAAGGGAT+GGG - Chr3:95618029-95618048 None:intergenic 45.0%
CTCAAAATCCGAGCTTGGTA+CGG - Chr3:95618197-95618216 None:intergenic 45.0%
TCCTCATACGGATCCAGTAA+GGG - Chr3:95618034-95618053 None:intergenic 45.0%
! CGATTGAAGAGACGCTGATT+TGG - Chr3:95617888-95617907 None:intergenic 45.0%
!! TGGTGTTGTCAGTCCTCATA+CGG - Chr3:95618046-95618065 None:intergenic 45.0%
AGGACCTCAAAATCCGAGCT+TGG - Chr3:95618202-95618221 None:intergenic 50.0%
CATACGGATCCAGTAAGGGA+TGG - Chr3:95618030-95618049 None:intergenic 50.0%
GTCCTCATACGGATCCAGTA+AGG - Chr3:95618035-95618054 None:intergenic 50.0%
TCCCTTACTGGATCCGTATG+AGG + Chr3:95618030-95618049 MsG0380017382.01.T01:CDS 50.0%
TGAGGACTGACAACACCATC+AGG + Chr3:95618048-95618067 MsG0380017382.01.T01:CDS 50.0%
! CCATTTTCGATCCTTGCCTC+CGG - Chr3:95617860-95617879 None:intergenic 50.0%
! CGTACCAAGCTCGGATTTTG+AGG + Chr3:95618195-95618214 MsG0380017382.01.T01:CDS 50.0%
CCGGAGGCAAGGATCGAAAA+TGG + Chr3:95617857-95617876 MsG0380017382.01.T01:exon 55.0%
GGACAAAACGCGGCAAAGCA+AGG - Chr3:95618222-95618241 None:intergenic 55.0%
TTGCCTCCGGAAATGCTCGA+CGG - Chr3:95617847-95617866 None:intergenic 55.0%
! ACAATGCTAAGCGCCGTCAC+TGG + Chr3:95618169-95618188 MsG0380017382.01.T01:CDS 55.0%
GTCGAGCATTTCCGGAGGCA+AGG + Chr3:95617846-95617865 MsG0380017382.01.T01:five_prime_UTR 60.0%
GTGCAGCCGTCGAGCATTTC+CGG + Chr3:95617838-95617857 MsG0380017382.01.T01:five_prime_UTR 60.0%
CACTGGCGCCGTACCAAGCT+CGG + Chr3:95618186-95618205 MsG0380017382.01.T01:CDS 65.0%
CAGCCGTCGAGCATTTCCGG+AGG + Chr3:95617841-95617860 MsG0380017382.01.T01:five_prime_UTR 65.0%
GCTTGGTACGGCGCCAGTGA+CGG - Chr3:95618185-95618204 None:intergenic 65.0%
Chromosome Type Strat End Strand Name
Chr3 gene 95617797 95618463 95617797 ID=MsG0380017382.01;Name=MsG0380017382.01
Chr3 mRNA 95617797 95618463 95617797 ID=MsG0380017382.01.T01;Parent=MsG0380017382.01;Name=MsG0380017382.01.T01;_AED=0.21;_eAED=0.21;_QI=79|1|1|1|0|0|3|248|51
Chr3 exon 95617797 95617878 95617797 ID=MsG0380017382.01.T01:exon:5870;Parent=MsG0380017382.01.T01
Chr3 exon 95617966 95618069 95617966 ID=MsG0380017382.01.T01:exon:5871;Parent=MsG0380017382.01.T01
Chr3 exon 95618167 95618463 95618167 ID=MsG0380017382.01.T01:exon:5872;Parent=MsG0380017382.01.T01
Chr3 five_prime_UTR 95617797 95617875 95617797 ID=MsG0380017382.01.T01:five_prime_utr;Parent=MsG0380017382.01.T01
Chr3 CDS 95617876 95617878 95617876 ID=MsG0380017382.01.T01:cds;Parent=MsG0380017382.01.T01
Chr3 CDS 95617966 95618069 95617966 ID=MsG0380017382.01.T01:cds;Parent=MsG0380017382.01.T01
Chr3 CDS 95618167 95618215 95618167 ID=MsG0380017382.01.T01:cds;Parent=MsG0380017382.01.T01
Chr3 three_prime_UTR 95618216 95618463 95618216 ID=MsG0380017382.01.T01:three_prime_utr;Parent=MsG0380017382.01.T01
Gene Sequence

>MsG0380017382.01.T01

ATGCCGTCACACAAGACATTCGTAATCAAGAAGAAATTGGCGAAGAAGATGAGGCAGAATAGACCCATCCCTTACTGGATCCGTATGAGGACTGACAACACCATCAGGTACAATGCTAAGCGCCGTCACTGGCGCCGTACCAAGCTCGGATTTTGA

Protein sequence

>MsG0380017382.01.T01

MPSHKTFVIKKKLAKKMRQNRPIPYWIRMRTDNTIRYNAKRRHWRRTKLGF*