Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0480023771.01.T01 | XP_003630655.1 | 98.45 | 129 | 2 | 0 | 1 | 129 | 1 | 129 | 4.02E-90 | 267 |
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0480023771.01.T01 | Q9M0E0 | 81.395 | 129 | 24 | 0 | 1 | 129 | 1 | 129 | 5.89E-78 | 229 |
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0480023771.01.T01 | B7FH41 | 98.450 | 129 | 2 | 0 | 1 | 129 | 1 | 129 | 1.92e-90 | 267 |
| Gene ID | Type | Classification |
|---|
| Gene ID | Type | Classification |
|---|
Co-expression Network
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MsG0080047968.01 | MsG0480023771.01 | 0.805401 | 1.358733e-49 | 7.181672e-47 |
| MsG0080048687.01 | MsG0480023771.01 | 0.840388 | 8.962225e-58 | 1.274675e-54 |
| MsG0080048971.01 | MsG0480023771.01 | 0.849353 | 3.415761e-60 | 6.458160e-57 |
| MsG0180000339.01 | MsG0480023771.01 | 0.835604 | 1.523071e-56 | 1.871736e-53 |
| MsG0180000575.01 | MsG0480023771.01 | 0.897832 | 9.592416e-77 | 1.103117e-72 |
| MsG0180000580.01 | MsG0480023771.01 | 0.824510 | 7.763599e-54 | 6.890939e-51 |
| MsG0180000614.01 | MsG0480023771.01 | 0.815914 | 7.241551e-52 | 5.058003e-49 |
| MsG0180000615.01 | MsG0480023771.01 | 0.803773 | 2.970763e-49 | 1.505773e-46 |
| MsG0180000743.01 | MsG0480023771.01 | 0.902046 | 1.446334e-78 | 2.010136e-74 |
| MsG0180002547.01 | MsG0480023771.01 | 0.831496 | 1.615268e-55 | 1.755856e-52 |
| MsG0180003793.01 | MsG0480023771.01 | 0.840842 | 6.813437e-58 | 9.828033e-55 |
| MsG0180004050.01 | MsG0480023771.01 | 0.844019 | 9.796988e-59 | 1.561089e-55 |
| MsG0180004481.01 | MsG0480023771.01 | 0.822356 | 2.474837e-53 | 2.066701e-50 |
| MsG0180004509.01 | MsG0480023771.01 | 0.866953 | 1.954464e-65 | 6.708192e-62 |
| MsG0180004854.01 | MsG0480023771.01 | 0.837073 | 6.445509e-57 | 8.278952e-54 |
| MsG0180004861.01 | MsG0480023771.01 | 0.896442 | 3.673316e-76 | 3.974505e-72 |
| MsG0180005366.01 | MsG0480023771.01 | 0.803435 | 3.490483e-49 | 1.753908e-46 |
| MsG0280006422.01 | MsG0480023771.01 | 0.814969 | 1.174878e-51 | 7.996988e-49 |
| MsG0280006424.01 | MsG0480023771.01 | 0.814874 | 1.233735e-51 | 8.375687e-49 |
| MsG0280010776.01 | MsG0480023771.01 | 0.807798 | 4.238812e-50 | 2.383183e-47 |
| MsG0280010801.01 | MsG0480023771.01 | 0.817583 | 3.058975e-52 | 2.236524e-49 |
| MsG0280011234.01 | MsG0480023771.01 | 0.841759 | 3.909725e-58 | 5.802409e-55 |
| MsG0380012073.01 | MsG0480023771.01 | 0.874591 | 5.992898e-68 | 2.712944e-64 |
| MsG0380014539.01 | MsG0480023771.01 | 0.808122 | 3.617157e-50 | 2.051060e-47 |
| MsG0380014797.01 | MsG0480023771.01 | 0.812409 | 4.301975e-51 | 2.733307e-48 |
| MsG0480018477.01 | MsG0480023771.01 | 0.833292 | 5.799261e-56 | 6.649358e-53 |
| MsG0480019670.01 | MsG0480023771.01 | 0.812406 | 4.307429e-51 | 2.736578e-48 |
| MsG0480021159.01 | MsG0480023771.01 | 0.907945 | 2.926544e-81 | 5.309560e-77 |
| MsG0480021453.01 | MsG0480023771.01 | 0.825226 | 5.260193e-54 | 4.765966e-51 |
| MsG0480021832.01 | MsG0480023771.01 | 0.808160 | 3.550748e-50 | 2.015487e-47 |
| MsG0480021842.01 | MsG0480023771.01 | 0.826609 | 2.469288e-54 | 2.326970e-51 |
| MsG0480022223.01 | MsG0480023771.01 | 0.850739 | 1.397274e-60 | 2.764076e-57 |
| MsG0480022614.01 | MsG0480023771.01 | 0.839031 | 2.020376e-57 | 2.755402e-54 |
| MsG0480022696.01 | MsG0480023771.01 | 0.853800 | 1.880859e-61 | 4.113125e-58 |
| MsG0480022790.01 | MsG0480023771.01 | 0.882629 | 8.876719e-71 | 5.444753e-67 |
| MsG0480022913.01 | MsG0480023771.01 | 0.864553 | 1.119379e-64 | 3.528484e-61 |
| MsG0480022960.01 | MsG0480023771.01 | 0.905674 | 3.343413e-80 | 5.466305e-76 |
| MsG0480023149.01 | MsG0480023771.01 | 0.824442 | 8.055039e-54 | 7.135181e-51 |
| MsG0480023221.01 | MsG0480023771.01 | 0.839147 | 1.884900e-57 | 2.579695e-54 |
| MsG0480023399.01 | MsG0480023771.01 | 0.869797 | 2.364557e-66 | 8.987022e-63 |
| MsG0480023467.01 | MsG0480023771.01 | 0.826899 | 2.105746e-54 | 2.001182e-51 |
| MsG0480023596.01 | MsG0480023771.01 | 0.823009 | 1.744290e-53 | 1.483892e-50 |
| MsG0480023738.01 | MsG0480023771.01 | 0.835238 | 1.885175e-56 | 2.291641e-53 |
| MsG0480023771.01 | MsG0480023844.01 | 0.863125 | 3.112185e-64 | 9.332774e-61 |
| MsG0480023771.01 | MsG0580024036.01 | 0.808107 | 3.644063e-50 | 2.065459e-47 |
| MsG0480023771.01 | MsG0580024369.01 | 0.845103 | 5.002908e-59 | 8.247946e-56 |
| MsG0480023771.01 | MsG0580024754.01 | 0.810846 | 9.408433e-51 | 5.733564e-48 |
| MsG0480023771.01 | MsG0580025268.01 | 0.812306 | 4.529766e-51 | 2.869956e-48 |
| MsG0480023771.01 | MsG0580025408.01 | 0.803365 | 3.609931e-49 | 1.810565e-46 |
| MsG0480023771.01 | MsG0580025756.01 | 0.803006 | 4.283300e-49 | 2.128592e-46 |
| MsG0480023771.01 | MsG0580026157.01 | 0.827055 | 1.932249e-54 | 1.844583e-51 |
| MsG0480023771.01 | MsG0580029132.01 | 0.854171 | 1.469856e-61 | 3.254541e-58 |
| MsG0480023771.01 | MsG0680031507.01 | 0.807532 | 4.827003e-50 | 2.695122e-47 |
| MsG0480023771.01 | MsG0680031628.01 | 0.803730 | 3.032426e-49 | 1.535339e-46 |
| MsG0480023771.01 | MsG0680032770.01 | 0.839096 | 1.944194e-57 | 2.656818e-54 |
| MsG0480023771.01 | MsG0680034671.01 | 0.835227 | 1.897636e-56 | 2.306035e-53 |
| MsG0480023771.01 | MsG0680035577.01 | 0.815558 | 8.695092e-52 | 6.014498e-49 |
| MsG0480023771.01 | MsG0680035848.01 | 0.823570 | 1.289893e-53 | 1.114770e-50 |
| MsG0480023771.01 | MsG0680035888.01 | 0.833215 | 6.060541e-56 | 6.933256e-53 |
| MsG0480023771.01 | MsG0780036546.01 | 0.806661 | 7.381995e-50 | 4.029408e-47 |
| MsG0480023771.01 | MsG0780037240.01 | 0.802671 | 5.022914e-49 | 2.475095e-46 |
| MsG0480023771.01 | MsG0780038400.01 | 0.852058 | 5.921654e-61 | 1.222451e-57 |
| MsG0480023771.01 | MsG0780038587.01 | 0.870840 | 1.076366e-66 | 4.248394e-63 |
| MsG0480023771.01 | MsG0780038735.01 | 0.806165 | 9.392911e-50 | 5.062966e-47 |
| MsG0480023771.01 | MsG0780038909.01 | 0.871970 | 4.553198e-67 | 1.872113e-63 |
| MsG0480023771.01 | MsG0780039844.01 | 0.804251 | 2.362411e-49 | 1.212167e-46 |
| MsG0480023771.01 | MsG0780039848.01 | 0.850305 | 1.850756e-60 | 3.609569e-57 |
| MsG0480023771.01 | MsG0780040162.01 | 0.801332 | 9.465488e-49 | 4.508271e-46 |
| MsG0480023771.01 | MsG0780040511.01 | 0.894464 | 2.406874e-75 | 2.395694e-71 |
| MsG0480023771.01 | MsG0780040782.01 | 0.832368 | 9.840544e-56 | 1.097589e-52 |
| MsG0480023771.01 | MsG0780041147.01 | 0.835302 | 1.816075e-56 | 2.212061e-53 |
| MsG0480023771.01 | MsG0780041223.01 | 0.857611 | 1.450827e-62 | 3.601993e-59 |
| MsG0480023771.01 | MsG0780041224.01 | 0.860359 | 2.182459e-63 | 5.949729e-60 |
| MsG0480023771.01 | MsG0780041295.01 | 0.802298 | 5.995212e-49 | 2.926428e-46 |
| MsG0480023771.01 | MsG0780041392.01 | 0.861652 | 8.823422e-64 | 2.514359e-60 |
| MsG0480023771.01 | MsG0780041462.01 | 0.845511 | 3.880676e-59 | 6.482216e-56 |
| MsG0480023771.01 | MsG0780041796.01 | 0.832169 | 1.102222e-55 | 1.222086e-52 |
| MsG0480023771.01 | MsG0880041967.01 | 0.839750 | 1.314184e-57 | 1.832221e-54 |
| MsG0480023771.01 | MsG0880042125.01 | 0.832730 | 7.999818e-56 | 9.019918e-53 |
| MsG0480023771.01 | MsG0880042131.01 | 0.863650 | 2.139680e-64 | 6.534974e-61 |
| MsG0480023771.01 | MsG0880042519.01 | 0.814162 | 1.772796e-51 | 1.180636e-48 |
| MsG0480023771.01 | MsG0880042715.01 | 0.813165 | 2.939094e-51 | 1.904959e-48 |
| MsG0480023771.01 | MsG0880042779.01 | 0.809010 | 2.337636e-50 | 1.356838e-47 |
| MsG0480023771.01 | MsG0880042991.01 | 0.836586 | 8.581328e-57 | 1.086161e-53 |
| MsG0480023771.01 | MsG0880043477.01 | 0.860485 | 1.999048e-63 | 5.473184e-60 |
| MsG0480023771.01 | MsG0880043770.01 | 0.861503 | 9.804246e-64 | 2.780374e-60 |
| MsG0480023771.01 | MsG0880044990.01 | 0.808505 | 2.997343e-50 | 1.716803e-47 |
| MsG0480023771.01 | MsG0880045072.01 | 0.835738 | 1.409034e-56 | 1.738643e-53 |
| MsG0480023771.01 | MsG0880045145.01 | 0.804527 | 2.069744e-49 | 1.069579e-46 |
| MsG0480023771.01 | MsG0880045656.01 | 0.828395 | 9.205468e-55 | 9.139052e-52 |
| MsG0480023771.01 | MsG0880045965.01 | 0.802779 | 4.771040e-49 | 2.357589e-46 |
| MsG0480023771.01 | MsG0880046177.01 | 0.803467 | 3.438427e-49 | 1.729145e-46 |
| MsG0380015027.01 | MsG0480023771.01 | 0.823077 | 1.682251e-53 | 1.433872e-50 |
| MsG0380015435.01 | MsG0480023771.01 | 0.837315 | 5.589907e-57 | 7.233257e-54 |
| MsG0380016067.01 | MsG0480023771.01 | 0.841243 | 5.347942e-58 | 7.809542e-55 |
| MsG0380016133.01 | MsG0480023771.01 | 0.803687 | 3.094900e-49 | 1.565236e-46 |
| MsG0380016290.01 | MsG0480023771.01 | 0.843815 | 1.111117e-58 | 1.759088e-55 |
| MsG0380016389.01 | MsG0480023771.01 | 0.837544 | 4.882921e-57 | 6.363376e-54 |
| MsG0380016559.01 | MsG0480023771.01 | 0.862693 | 4.230587e-64 | 1.249970e-60 |
| MsG0380016560.01 | MsG0480023771.01 | 0.846472 | 2.125805e-59 | 3.663462e-56 |
| MsG0380016604.01 | MsG0480023771.01 | 0.836922 | 7.044719e-57 | 9.007611e-54 |
| MsG0380016775.01 | MsG0480023771.01 | 0.917886 | 3.070633e-86 | 9.081618e-82 |
| MsG0380016778.01 | MsG0480023771.01 | 0.800457 | 1.428296e-48 | 6.653625e-46 |
| MsG0380016860.01 | MsG0480023771.01 | 0.843433 | 1.405394e-58 | 2.198528e-55 |
| MsG0380017382.01 | MsG0480023771.01 | 0.923805 | 1.634394e-89 | 6.673712e-85 |
| MsG0380017540.01 | MsG0480023771.01 | 0.807433 | 5.067428e-50 | 2.822056e-47 |
| MsG0380017947.01 | MsG0480023771.01 | 0.815338 | 9.728219e-52 | 6.688901e-49 |
| MsG0380018033.01 | MsG0480023771.01 | 0.822274 | 2.586384e-53 | 2.154715e-50 |
| MsG0480018084.01 | MsG0480023771.01 | 0.831128 | 1.989264e-55 | 2.138948e-52 |
| MsG0480018110.01 | MsG0480023771.01 | 0.806817 | 6.841997e-50 | 3.749998e-47 |
| MsG0280006483.01 | MsG0480023771.01 | 0.878630 | 2.404135e-69 | 1.264602e-65 |
| MsG0280006593.01 | MsG0480023771.01 | 0.811579 | 6.524252e-51 | 4.053528e-48 |
| MsG0280006836.01 | MsG0480023771.01 | 0.822516 | 2.271893e-53 | 1.905916e-50 |
| MsG0280006993.01 | MsG0480023771.01 | 0.849814 | 2.538704e-60 | 4.872246e-57 |
| MsG0280007024.01 | MsG0480023771.01 | 0.810498 | 1.118722e-50 | 6.754168e-48 |
| MsG0280007321.01 | MsG0480023771.01 | 0.801510 | 8.704904e-49 | 4.164690e-46 |
| MsG0280007787.01 | MsG0480023771.01 | 0.811961 | 5.388696e-51 | 3.382145e-48 |
| MsG0280007847.01 | MsG0480023771.01 | 0.839892 | 1.207554e-57 | 1.691087e-54 |
| MsG0280008118.01 | MsG0480023771.01 | 0.803463 | 3.444217e-49 | 1.731940e-46 |
| MsG0280009540.01 | MsG0480023771.01 | 0.805826 | 1.106775e-49 | 5.914138e-47 |
| MsG0280009575.01 | MsG0480023771.01 | 0.826895 | 2.110129e-54 | 2.005090e-51 |
| MsG0280010525.01 | MsG0480023771.01 | 0.817575 | 3.070997e-52 | 2.244808e-49 |
PPI
| Gene1 | Gene2 | Type |
|---|---|---|
| MsG0480023771.01 | MsG0880046933.01 | PPI |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0480023771.01.T01 | MTR_8g101910 | 98.450 | 129 | 2 | 0 | 1 | 129 | 1 | 129 | 4.86e-94 | 267 |
| MsG0480023771.01.T01 | MTR_8g101910 | 96.970 | 99 | 3 | 0 | 1 | 99 | 1 | 99 | 3.55e-68 | 201 |
| MsG0480023771.01.T01 | MTR_4g094598 | 52.000 | 125 | 60 | 0 | 5 | 129 | 6 | 130 | 8.07e-46 | 145 |
| MsG0480023771.01.T01 | MTR_1g105415 | 52.000 | 125 | 60 | 0 | 5 | 129 | 6 | 130 | 8.07e-46 | 145 |
| MsG0480023771.01.T01 | MTR_4g117470 | 52.000 | 125 | 60 | 0 | 5 | 129 | 6 | 130 | 2.07e-45 | 144 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0480023771.01.T01 | AT4G29430 | 81.395 | 129 | 24 | 0 | 1 | 129 | 1 | 129 | 6.01e-79 | 229 |
| MsG0480023771.01.T01 | AT2G19720 | 79.845 | 129 | 26 | 0 | 1 | 129 | 1 | 129 | 1.67e-77 | 225 |
| MsG0480023771.01.T01 | AT2G39590 | 52.000 | 125 | 60 | 0 | 5 | 129 | 6 | 130 | 3.64e-46 | 146 |
| MsG0480023771.01.T01 | AT5G59850 | 52.000 | 125 | 60 | 0 | 5 | 129 | 6 | 130 | 2.05e-45 | 144 |
| MsG0480023771.01.T01 | AT1G07770 | 52.000 | 125 | 60 | 0 | 5 | 129 | 6 | 130 | 2.05e-45 | 144 |
| MsG0480023771.01.T01 | AT1G07770 | 52.000 | 125 | 60 | 0 | 5 | 129 | 6 | 130 | 2.05e-45 | 144 |
| MsG0480023771.01.T01 | AT1G07770 | 52.000 | 125 | 60 | 0 | 5 | 129 | 6 | 130 | 2.05e-45 | 144 |
| MsG0480023771.01.T01 | AT3G46040 | 50.400 | 125 | 62 | 0 | 5 | 129 | 6 | 130 | 2.86e-44 | 141 |
Find 62 sgRNAs with CRISPR-Local
Find 108 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| TGTCGTTTACCTTGTTATTT+AGG | 0.207524 | 4:-89606200 | MsG0480023771.01.T01:three_prime_UTR |
| ATCACAATCTTTGTGGAAAT+TGG | 0.217738 | 4:-89606037 | MsG0480023771.01.T01:three_prime_UTR |
| CTGTGATCCTCCCCACTCTA+TGG | 0.263185 | 4:+89606606 | None:intergenic |
| GAAGAGATAACATTGGAAAT+AGG | 0.265767 | 4:+89607265 | None:intergenic |
| TACAACTCCTGATGGTGTTT+TGG | 0.307095 | 4:-89606285 | MsG0480023771.01.T01:CDS |
| AATGTAGGTGGTCAGGTTCT+TGG | 0.331557 | 4:-89606239 | MsG0480023771.01.T01:CDS |
| AAATCCATTGAAAGCTTATC+AGG | 0.331884 | 4:+89606152 | None:intergenic |
| TGAGGTCTTGTCGGTATGTA+AGG | 0.347848 | 4:+89606549 | None:intergenic |
| ATCGAGTGAGAAAGAGAAAT+GGG | 0.386148 | 4:-89607364 | MsG0480023771.01.T01:exon |
| AGGATATATCAAGGACTTTC+AGG | 0.389863 | 4:-89606639 | MsG0480023771.01.T01:CDS |
| CCTTTGCCTTGAGGTCTTGT+CGG | 0.398017 | 4:+89606540 | None:intergenic |
| ATCATGAAGAGGCCGCAAAA+AGG | 0.404793 | 4:-89606262 | MsG0480023771.01.T01:CDS |
| GTTCGTTAATCGTGAAATAG+TGG | 0.410013 | 4:+89607448 | None:intergenic |
| AAACCGTTCTAGCTTGGCTT+TGG | 0.416322 | 4:-89607424 | MsG0480023771.01.T01:five_prime_UTR |
| GTTTCCTGATAAGCTTTCAA+TGG | 0.417059 | 4:-89606156 | MsG0480023771.01.T01:three_prime_UTR |
| GATCGAGTGAGAAAGAGAAA+TGG | 0.452370 | 4:-89607365 | MsG0480023771.01.T01:exon |
| TGTGATCCTCCCCACTCTAT+GGG | 0.457890 | 4:+89606607 | None:intergenic |
| TTCAACATTGATTATCCTCT+AGG | 0.463340 | 4:+89605829 | None:intergenic |
| AATAATAGACTAGACTTGAA+TGG | 0.465985 | 4:-89606000 | MsG0480023771.01.T01:three_prime_UTR |
| CGAACAAAACCGTTCTAGCT+TGG | 0.470138 | 4:-89607430 | MsG0480023771.01.T01:five_prime_UTR |
| ATTGCAGCGTCGCGTGGTAG+CGG | 0.474245 | 4:-89607500 | MsG0480023771.01.T01:five_prime_UTR |
| GAACGATGCGTTGAGGAGTA+TGG | 0.475978 | 4:-89607329 | MsG0480023771.01.T01:CDS |
| GAACACTCTTCCAACACATC+AGG | 0.488180 | 4:-89606498 | MsG0480023771.01.T01:intron |
| TCATGATCCAAAACACCATC+AGG | 0.488311 | 4:+89606278 | None:intergenic |
| GCTTGGCTTTGGCATACTGT+TGG | 0.493184 | 4:-89607413 | MsG0480023771.01.T01:five_prime_UTR |
| AAGAAAAGAAGAGATAACAT+TGG | 0.493444 | 4:+89607258 | None:intergenic |
| GAGAAAGAGAAATGGGGAGA+AGG | 0.505773 | 4:-89607357 | MsG0480023771.01.T01:exon |
| CATTAACCAACCTGATGTGT+TGG | 0.507241 | 4:+89606488 | None:intergenic |
| GCGTTGGATCACAATCTTTG+TGG | 0.531472 | 4:-89606044 | MsG0480023771.01.T01:three_prime_UTR |
| GCAGCGTCGCGTGGTAGCGG+CGG | 0.533603 | 4:-89607497 | MsG0480023771.01.T01:five_prime_UTR |
| AACTGCCACATAATTCATAC+AGG | 0.539779 | 4:+89606077 | None:intergenic |
| ATTCAATATCCTTTGCCTTG+AGG | 0.544391 | 4:+89606531 | None:intergenic |
| TTTGTCCTGTATGAATTATG+TGG | 0.561597 | 4:-89606082 | MsG0480023771.01.T01:three_prime_UTR |
| GAAGAGAGGCAAAGCTATGG+TGG | 0.562394 | 4:-89607296 | MsG0480023771.01.T01:CDS |
| GAGGTCTTGTCGGTATGTAA+GGG | 0.568541 | 4:+89606550 | None:intergenic |
| AGATTACAACTAAGATGATA+CGG | 0.572731 | 4:+89605974 | None:intergenic |
| GCAGTTCATGCATGATGCGT+TGG | 0.574386 | 4:-89606060 | MsG0480023771.01.T01:three_prime_UTR |
| ATGCCAAAGCCAAGCTAGAA+CGG | 0.574745 | 4:+89607421 | None:intergenic |
| GTGTATGATCCCCATAGAGT+GGG | 0.576109 | 4:-89606617 | MsG0480023771.01.T01:CDS |
| CATGTTGGCAGGATATATCA+AGG | 0.581279 | 4:-89606648 | MsG0480023771.01.T01:intron |
| ACTTAGCTGAAGACAGTGAC+TGG | 0.585077 | 4:+89606177 | None:intergenic |
| TCGAGTGAGAAAGAGAAATG+GGG | 0.585958 | 4:-89607363 | MsG0480023771.01.T01:exon |
| AGAAAACTAGTGTTGCAGAA+TGG | 0.589693 | 4:+89605902 | None:intergenic |
| GTTCAAAGAGGGTTGAAAGA+CGG | 0.593795 | 4:-89607535 | None:intergenic |
| TGAGAAGAGAGGCAAAGCTA+TGG | 0.613395 | 4:-89607299 | MsG0480023771.01.T01:CDS |
| GGTGTATGATCCCCATAGAG+TGG | 0.614660 | 4:-89606618 | MsG0480023771.01.T01:CDS |
| AGGTAGAATTAACGATTGCA+AGG | 0.617470 | 4:-89606573 | MsG0480023771.01.T01:CDS |
| AGAGTGATTGCAGCGTCGCG+TGG | 0.630341 | 4:-89607506 | MsG0480023771.01.T01:five_prime_UTR |
| ATACTGTTGGTGAAAACTAT+CGG | 0.645207 | 4:-89607400 | MsG0480023771.01.T01:five_prime_UTR |
| TACATACCGACAAGACCTCA+AGG | 0.646826 | 4:-89606546 | MsG0480023771.01.T01:CDS |
| CCGACAAGACCTCAAGGCAA+AGG | 0.650773 | 4:-89606540 | MsG0480023771.01.T01:CDS |
| GGATACTGAACGATGCGTTG+AGG | 0.657571 | 4:-89607336 | MsG0480023771.01.T01:CDS |
| AGGATCACAGTTGAACTACA+AGG | 0.660202 | 4:-89606593 | MsG0480023771.01.T01:CDS |
| AGAATAATATATCATCCTAG+AGG | 0.668397 | 4:-89605844 | MsG0480023771.01.T01:three_prime_UTR |
| ATGGTGAATGCTGAGAAGAG+AGG | 0.672519 | 4:-89607310 | MsG0480023771.01.T01:CDS |
| AGTGACTGGCCTAAATAACA+AGG | 0.673320 | 4:+89606191 | None:intergenic |
| GTTGTGATTACAACTCCTGA+TGG | 0.673437 | 4:-89606293 | MsG0480023771.01.T01:CDS |
| GTGATCCTCCCCACTCTATG+GGG | 0.676832 | 4:+89606608 | None:intergenic |
| TGTATGATCCCCATAGAGTG+GGG | 0.683001 | 4:-89606616 | MsG0480023771.01.T01:CDS |
| TGAAATAGTGGTGTTGCCAG+TGG | 0.689997 | 4:+89607460 | None:intergenic |
| ATGATCCCCATAGAGTGGGG+AGG | 0.698167 | 4:-89606613 | MsG0480023771.01.T01:CDS |
| CTTCAAATCATGAAACACCG+CGG | 0.732704 | 4:-89607238 | MsG0480023771.01.T01:intron |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !!! | ATTTTTAAAGATATTTTTAG+TGG | - | Chr4:89606347-89606366 | MsG0480023771.01.T01:intron | 10.0% |
| !! | AACACTAAATAAAAACAAAA+CGG | + | Chr4:89606892-89606911 | None:intergenic | 15.0% |
| !!! | AGAATTTAAAATATAAAAGC+TGG | + | Chr4:89606964-89606983 | None:intergenic | 15.0% |
| !!! | TTTTTAATAGATATGAGTAA+AGG | - | Chr4:89606987-89607006 | MsG0480023771.01.T01:intron | 15.0% |
| !! | ATGTCTTAATTAATGGAATT+TGG | + | Chr4:89606283-89606302 | None:intergenic | 20.0% |
| !! | CATTAATTAAGACATACATT+AGG | - | Chr4:89606288-89606307 | MsG0480023771.01.T01:CDS | 20.0% |
| !! | CTAATGTATGTCTTAATTAA+TGG | + | Chr4:89606290-89606309 | None:intergenic | 20.0% |
| !! | TGTCTTAATTAATGGAATTT+GGG | + | Chr4:89606282-89606301 | None:intergenic | 20.0% |
| ! | AAGAAAAGAAGAGATAACAT+TGG | + | Chr4:89606095-89606114 | None:intergenic | 25.0% |
| ! | AATAATAGACTAGACTTGAA+TGG | - | Chr4:89607350-89607369 | MsG0480023771.01.T01:exon | 25.0% |
| ! | AATTCAAACAAATATTACCG+CGG | + | Chr4:89606132-89606151 | None:intergenic | 25.0% |
| ! | AGAATAATATATCATCCTAG+AGG | - | Chr4:89607506-89607525 | MsG0480023771.01.T01:five_prime_UTR | 25.0% |
| ! | AGATTACAACTAAGATGATA+CGG | + | Chr4:89607379-89607398 | None:intergenic | 25.0% |
| ! | ATAGATATGAGTAAAGGAAA+AGG | - | Chr4:89606993-89607012 | MsG0480023771.01.T01:intron | 25.0% |
| ! | ATTTCATACTCATAAGATGA+AGG | + | Chr4:89606183-89606202 | None:intergenic | 25.0% |
| ! | TAATTGTTGGAAAATAACCA+TGG | + | Chr4:89606562-89606581 | None:intergenic | 25.0% |
| ! | TCATATCTCATGAAAAGAAA+TGG | - | Chr4:89606533-89606552 | MsG0480023771.01.T01:CDS | 25.0% |
| ! | TTACTTCCATTATCTCAAAT+TGG | - | Chr4:89606464-89606483 | MsG0480023771.01.T01:intron | 25.0% |
| ! | TTTATTTATCTTGATGCAGT+GGG | - | Chr4:89607029-89607048 | MsG0480023771.01.T01:intron | 25.0% |
| ! | TTTCATACTCATAAGATGAA+GGG | + | Chr4:89606182-89606201 | None:intergenic | 25.0% |
| !!! | TGTGTTTTGGATAATTCATT+AGG | - | Chr4:89606232-89606251 | MsG0480023771.01.T01:CDS | 25.0% |
| !!! | TTTTATTTATCTTGATGCAG+TGG | - | Chr4:89607028-89607047 | MsG0480023771.01.T01:intron | 25.0% |
| AAATCCATTGAAAGCTTATC+AGG | + | Chr4:89607201-89607220 | None:intergenic | 30.0% | |
| ACAAATATAATCACGTGTGT+AGG | + | Chr4:89606444-89606463 | None:intergenic | 30.0% | |
| AGCATGTATATGTACATGAA+AGG | - | Chr4:89606618-89606637 | MsG0480023771.01.T01:CDS | 30.0% | |
| AGCATTCTCCATATACAAAT+AGG | + | Chr4:89606927-89606946 | None:intergenic | 30.0% | |
| ATCACAATCTTTGTGGAAAT+TGG | - | Chr4:89607313-89607332 | MsG0480023771.01.T01:CDS | 30.0% | |
| CAGTTTCTCCTATTTGTATA+TGG | - | Chr4:89606916-89606935 | MsG0480023771.01.T01:intron | 30.0% | |
| GAAGAGATAACATTGGAAAT+AGG | + | Chr4:89606088-89606107 | None:intergenic | 30.0% | |
| TTATTTATCTTGATGCAGTG+GGG | - | Chr4:89607030-89607049 | MsG0480023771.01.T01:intron | 30.0% | |
| TTTGTCCTGTATGAATTATG+TGG | - | Chr4:89607268-89607287 | MsG0480023771.01.T01:CDS | 30.0% | |
| ! | ATACTGTTGGTGAAAACTAT+CGG | - | Chr4:89605950-89605969 | MsG0480023771.01.T01:three_prime_UTR | 30.0% |
| ! | ATTTTCCAACAATTATCAGC+TGG | - | Chr4:89606567-89606586 | MsG0480023771.01.T01:CDS | 30.0% |
| ! | GTCATACCAATTTGAGATAA+TGG | + | Chr4:89606473-89606492 | None:intergenic | 30.0% |
| ! | TGAAAGAATGTTTTCAGCAA+AGG | - | Chr4:89607223-89607242 | MsG0480023771.01.T01:intron | 30.0% |
| ! | TTCAACATTGATTATCCTCT+AGG | + | Chr4:89607524-89607543 | None:intergenic | 30.0% |
| !! | TGTCGTTTACCTTGTTATTT+AGG | - | Chr4:89607150-89607169 | MsG0480023771.01.T01:intron | 30.0% |
| !! | TTTTCTTATTTGTTTTCGCG+AGG | - | Chr4:89607465-89607484 | MsG0480023771.01.T01:five_prime_UTR | 30.0% |
| !!! | TATGTATTCGTTTTTTGAGC+TGG | - | Chr4:89606204-89606223 | MsG0480023771.01.T01:three_prime_UTR | 30.0% |
| AACTGCCACATAATTCATAC+AGG | + | Chr4:89607276-89607295 | None:intergenic | 35.0% | |
| AATTATCAGCTGGATAAGCA+TGG | - | Chr4:89606577-89606596 | MsG0480023771.01.T01:CDS | 35.0% | |
| AGAAAACTAGTGTTGCAGAA+TGG | + | Chr4:89607451-89607470 | None:intergenic | 35.0% | |
| AGGATATATCAAGGACTTTC+AGG | - | Chr4:89606711-89606730 | MsG0480023771.01.T01:intron | 35.0% | |
| AGGTAGAATTAACGATTGCA+AGG | - | Chr4:89606777-89606796 | MsG0480023771.01.T01:intron | 35.0% | |
| ATCGAGTGAGAAAGAGAAAT+GGG | - | Chr4:89605986-89606005 | MsG0480023771.01.T01:three_prime_UTR | 35.0% | |
| ATGAAAAGAAATGGTCTCCA+TGG | - | Chr4:89606542-89606561 | MsG0480023771.01.T01:CDS | 35.0% | |
| ATTCAATATCCTTTGCCTTG+AGG | + | Chr4:89606822-89606841 | None:intergenic | 35.0% | |
| CTTATCCAGCTGATAATTGT+TGG | + | Chr4:89606575-89606594 | None:intergenic | 35.0% | |
| GCTACATAGTTCTTGAGAAA+TGG | + | Chr4:89606381-89606400 | None:intergenic | 35.0% | |
| GTTCGTTAATCGTGAAATAG+TGG | + | Chr4:89605905-89605924 | None:intergenic | 35.0% | |
| GTTTCCTGATAAGCTTTCAA+TGG | - | Chr4:89607194-89607213 | MsG0480023771.01.T01:intron | 35.0% | |
| ! | AAGAATGTTTTCAGCAAAGG+AGG | - | Chr4:89607226-89607245 | MsG0480023771.01.T01:intron | 35.0% |
| ! | TGAGCTGGATTTATGTGTTT+TGG | - | Chr4:89606219-89606238 | MsG0480023771.01.T01:exon | 35.0% |
| !! | TAATTTGCTGTCGTTCATGT+TGG | - | Chr4:89606687-89606706 | MsG0480023771.01.T01:intron | 35.0% |
| AAAAAGGAATGTAGGTGGTC+AGG | - | Chr4:89607104-89607123 | MsG0480023771.01.T01:intron | 40.0% | |
| AGGATCACAGTTGAACTACA+AGG | - | Chr4:89606757-89606776 | MsG0480023771.01.T01:intron | 40.0% | |
| CATGTTGGCAGGATATATCA+AGG | - | Chr4:89606702-89606721 | MsG0480023771.01.T01:intron | 40.0% | |
| CATTAACCAACCTGATGTGT+TGG | + | Chr4:89606865-89606884 | None:intergenic | 40.0% | |
| CTCAAATTGGTATGACGTTG+AGG | - | Chr4:89606477-89606496 | MsG0480023771.01.T01:intron | 40.0% | |
| CTTCAAATCATGAAACACCG+CGG | - | Chr4:89606112-89606131 | MsG0480023771.01.T01:three_prime_UTR | 40.0% | |
| GATCGAGTGAGAAAGAGAAA+TGG | - | Chr4:89605985-89606004 | MsG0480023771.01.T01:three_prime_UTR | 40.0% | |
| GTTGTGATTACAACTCCTGA+TGG | - | Chr4:89607057-89607076 | MsG0480023771.01.T01:intron | 40.0% | |
| TCATGATCCAAAACACCATC+AGG | + | Chr4:89607075-89607094 | None:intergenic | 40.0% | |
| TCGAGTGAGAAAGAGAAATG+GGG | - | Chr4:89605987-89606006 | MsG0480023771.01.T01:three_prime_UTR | 40.0% | |
| ! | ACCACCTACATTCCTTTTTG+CGG | + | Chr4:89607103-89607122 | None:intergenic | 40.0% |
| !! | AGTGACTGGCCTAAATAACA+AGG | + | Chr4:89607162-89607181 | None:intergenic | 40.0% |
| !! | TACAACTCCTGATGGTGTTT+TGG | - | Chr4:89607065-89607084 | MsG0480023771.01.T01:intron | 40.0% |
| !! | TGGTGTTTTGGATCATGAAG+AGG | - | Chr4:89607077-89607096 | MsG0480023771.01.T01:intron | 40.0% |
| AATGTAGGTGGTCAGGTTCT+TGG | - | Chr4:89607111-89607130 | MsG0480023771.01.T01:intron | 45.0% | |
| ACTCTTCCAACACATCAGGT+TGG | - | Chr4:89606856-89606875 | MsG0480023771.01.T01:intron | 45.0% | |
| ACTTAGCTGAAGACAGTGAC+TGG | + | Chr4:89607176-89607195 | None:intergenic | 45.0% | |
| ATCATGAAGAGGCCGCAAAA+AGG | - | Chr4:89607088-89607107 | MsG0480023771.01.T01:intron | 45.0% | |
| ATGCCAAAGCCAAGCTAGAA+CGG | + | Chr4:89605932-89605951 | None:intergenic | 45.0% | |
| ATGGTGAATGCTGAGAAGAG+AGG | - | Chr4:89606040-89606059 | MsG0480023771.01.T01:three_prime_UTR | 45.0% | |
| ATTGGTATGACGTTGAGGTG+TGG | - | Chr4:89606482-89606501 | MsG0480023771.01.T01:intron | 45.0% | |
| GAACACTCTTCCAACACATC+AGG | - | Chr4:89606852-89606871 | MsG0480023771.01.T01:intron | 45.0% | |
| GAGAAAGAGAAATGGGGAGA+AGG | - | Chr4:89605993-89606012 | MsG0480023771.01.T01:three_prime_UTR | 45.0% | |
| GCGTTGGATCACAATCTTTG+TGG | - | Chr4:89607306-89607325 | MsG0480023771.01.T01:CDS | 45.0% | |
| GTGTATGATCCCCATAGAGT+GGG | - | Chr4:89606733-89606752 | MsG0480023771.01.T01:intron | 45.0% | |
| TACATACCGACAAGACCTCA+AGG | - | Chr4:89606804-89606823 | MsG0480023771.01.T01:intron | 45.0% | |
| TGAGAAGAGAGGCAAAGCTA+TGG | - | Chr4:89606051-89606070 | MsG0480023771.01.T01:three_prime_UTR | 45.0% | |
| TGTATGATCCCCATAGAGTG+GGG | - | Chr4:89606734-89606753 | MsG0480023771.01.T01:intron | 45.0% | |
| TTGGTATGACGTTGAGGTGT+GGG | - | Chr4:89606483-89606502 | MsG0480023771.01.T01:intron | 45.0% | |
| ! | CGAACAAAACCGTTCTAGCT+TGG | - | Chr4:89605920-89605939 | MsG0480023771.01.T01:three_prime_UTR | 45.0% |
| ! | GAGGTCTTGTCGGTATGTAA+GGG | + | Chr4:89606803-89606822 | None:intergenic | 45.0% |
| ! | TGAGGTCTTGTCGGTATGTA+AGG | + | Chr4:89606804-89606823 | None:intergenic | 45.0% |
| !! | AAACCGTTCTAGCTTGGCTT+TGG | - | Chr4:89605926-89605945 | MsG0480023771.01.T01:three_prime_UTR | 45.0% |
| !! | TGAAATAGTGGTGTTGCCAG+TGG | + | Chr4:89605893-89605912 | None:intergenic | 45.0% |
| !!! | TATTATTAATTTTAGATTTT+TGG | - | Chr4:89606312-89606331 | MsG0480023771.01.T01:intron | 5.0% |
| GAACGATGCGTTGAGGAGTA+TGG | - | Chr4:89606021-89606040 | MsG0480023771.01.T01:three_prime_UTR | 50.0% | |
| GAAGAGAGGCAAAGCTATGG+TGG | - | Chr4:89606054-89606073 | MsG0480023771.01.T01:three_prime_UTR | 50.0% | |
| GAGGCCGCAAAAAGGAATGT+AGG | - | Chr4:89607096-89607115 | MsG0480023771.01.T01:intron | 50.0% | |
| GCAGTTCATGCATGATGCGT+TGG | - | Chr4:89607290-89607309 | MsG0480023771.01.T01:CDS | 50.0% | |
| GCCGCAAAAAGGAATGTAGG+TGG | - | Chr4:89607099-89607118 | MsG0480023771.01.T01:intron | 50.0% | |
| GGATACTGAACGATGCGTTG+AGG | - | Chr4:89606014-89606033 | MsG0480023771.01.T01:three_prime_UTR | 50.0% | |
| GGTGTATGATCCCCATAGAG+TGG | - | Chr4:89606732-89606751 | MsG0480023771.01.T01:intron | 50.0% | |
| TGTGATCCTCCCCACTCTAT+GGG | + | Chr4:89606746-89606765 | None:intergenic | 50.0% | |
| TTGCTGTCGTTCATGTTGGC+AGG | - | Chr4:89606691-89606710 | MsG0480023771.01.T01:intron | 50.0% | |
| ! | CCTTTGCCTTGAGGTCTTGT+CGG | + | Chr4:89606813-89606832 | None:intergenic | 50.0% |
| ! | GGCGAACATTTTCTGACCAC+TGG | - | Chr4:89605874-89605893 | MsG0480023771.01.T01:three_prime_UTR | 50.0% |
| !! | GCTTGGCTTTGGCATACTGT+TGG | - | Chr4:89605937-89605956 | MsG0480023771.01.T01:three_prime_UTR | 50.0% |
| ATGATCCCCATAGAGTGGGG+AGG | - | Chr4:89606737-89606756 | MsG0480023771.01.T01:intron | 55.0% | |
| CCGACAAGACCTCAAGGCAA+AGG | - | Chr4:89606810-89606829 | MsG0480023771.01.T01:intron | 55.0% | |
| CTGTGATCCTCCCCACTCTA+TGG | + | Chr4:89606747-89606766 | None:intergenic | 55.0% | |
| GTGATCCTCCCCACTCTATG+GGG | + | Chr4:89606745-89606764 | None:intergenic | 55.0% | |
| AGAGTGATTGCAGCGTCGCG+TGG | - | Chr4:89605844-89605863 | MsG0480023771.01.T01:three_prime_UTR | 60.0% | |
| ATTGCAGCGTCGCGTGGTAG+CGG | - | Chr4:89605850-89605869 | MsG0480023771.01.T01:three_prime_UTR | 60.0% | |
| !! | GCAGCGTCGCGTGGTAGCGG+CGG | - | Chr4:89605853-89605872 | MsG0480023771.01.T01:three_prime_UTR | 75.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| Chr4 | gene | 89605819 | 89607553 | 89605819 | ID=MsG0480023771.01;Name=MsG0480023771.01 |
| Chr4 | mRNA | 89605819 | 89607553 | 89605819 | ID=MsG0480023771.01.T01;Parent=MsG0480023771.01;Name=MsG0480023771.01.T01;_AED=0.26;_eAED=0.26;_QI=185|1|1|1|1|1|3|407|129 |
| Chr4 | exon | 89605819 | 89606324 | 89605819 | ID=MsG0480023771.01.T01:exon:25360;Parent=MsG0480023771.01.T01 |
| Chr4 | exon | 89606499 | 89606659 | 89606499 | ID=MsG0480023771.01.T01:exon:25359;Parent=MsG0480023771.01.T01 |
| Chr4 | exon | 89607239 | 89607553 | 89607239 | ID=MsG0480023771.01.T01:exon:25358;Parent=MsG0480023771.01.T01 |
| Chr4 | five_prime_UTR | 89607369 | 89607553 | 89607369 | ID=MsG0480023771.01.T01:five_prime_utr;Parent=MsG0480023771.01.T01 |
| Chr4 | CDS | 89607239 | 89607368 | 89607239 | ID=MsG0480023771.01.T01:cds;Parent=MsG0480023771.01.T01 |
| Chr4 | CDS | 89606499 | 89606659 | 89606499 | ID=MsG0480023771.01.T01:cds;Parent=MsG0480023771.01.T01 |
| Chr4 | CDS | 89606226 | 89606324 | 89606226 | ID=MsG0480023771.01.T01:cds;Parent=MsG0480023771.01.T01 |
| Chr4 | three_prime_UTR | 89605819 | 89606225 | 89605819 | ID=MsG0480023771.01.T01:three_prime_utr;Parent=MsG0480023771.01.T01 |
| Gene Sequence |
| Protein sequence |
