AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar ZhongmuNo.1 / MsG0880045951.01


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsG0880045951.01.T01 MTR_4g095390 97.436 39 1 0 1 39 1 39 3.08e-19 73.9
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsG0880045951.01.T01 AT2G23090 79.487 39 8 0 1 39 1 39 3.66e-14 61.2

Find 25 sgRNAs with CRISPR-Local

Find 67 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
CTTCTTCCGATAAAGAAATT+AGG 0.147345 8:+66843017 MsG0880045951.01.T01:five_prime_UTR
CAAAACCCTAATTTCTTTAT+CGG 0.268738 8:-66843023 None:intergenic
TTCTTCCGATAAAGAAATTA+GGG 0.339605 8:+66843018 MsG0880045951.01.T01:five_prime_UTR
ATTCGTTATGTTAGGGAGTT+GGG 0.362100 8:+66843754 MsG0880045951.01.T01:intron
ATAAAGGGAAAGGAATCTAA+TGG 0.394592 8:+66843789 MsG0880045951.01.T01:CDS
TTATGTTAGGGAGTTGGGTT+GGG 0.396722 8:+66843759 MsG0880045951.01.T01:intron
CGAACAAGTTAAAGAATTTG+AGG 0.402928 8:+66843830 MsG0880045951.01.T01:CDS
TTGAGGGTCTCTCTCAGCCA+TGG 0.415376 8:+66843043 MsG0880045951.01.T01:exon
AATTCGTTATGTTAGGGAGT+TGG 0.455871 8:+66843753 MsG0880045951.01.T01:intron
TAAAGGGAAAGGAATCTAAT+GGG 0.476026 8:+66843790 MsG0880045951.01.T01:CDS
TGAGGGTCTCTCTCAGCCAT+GGG 0.480356 8:+66843044 MsG0880045951.01.T01:exon
TGTGCGCAAAACGAAAATAA+TGG 0.480751 8:-66842985 None:intergenic
GTTATGTTAGGGAGTTGGGT+TGG 0.480955 8:+66843758 MsG0880045951.01.T01:intron
GAACAAGTTAAAGAATTTGA+GGG 0.485386 8:+66843831 MsG0880045951.01.T01:CDS
TGGGTTGGGAGAAAATATAA+AGG 0.489209 8:+66843773 MsG0880045951.01.T01:CDS
CTCGAGAAGCAGAAGAATGC+TGG 0.496068 8:+66843110 MsG0880045951.01.T01:CDS
CAGCATTCTTCTGCTTCTCG+AGG 0.532542 8:-66843109 None:intergenic
TCTCTCTCAGCCATGGGTGG+AGG 0.540024 8:+66843050 MsG0880045951.01.T01:exon
TTGACCGTTACCTCCACCCA+TGG 0.542711 8:-66843060 None:intergenic
TCAGCCATGGGTGGAGGTAA+CGG 0.548723 8:+66843056 MsG0880045951.01.T01:exon
GGGTTGGGAGAAAATATAAA+GGG 0.552173 8:+66843774 MsG0880045951.01.T01:CDS
GGGTCTCTCTCAGCCATGGG+TGG 0.552601 8:+66843047 MsG0880045951.01.T01:exon
GGGAGAAAATATAAAGGGAA+AGG 0.566626 8:+66843779 MsG0880045951.01.T01:CDS
AAGCAGAAGAATGCTGGCAA+AGG 0.595797 8:+66843116 MsG0880045951.01.T01:CDS
AAGCTAAGATGGCTCGCGAG+AGG 0.616608 8:+66843084 MsG0880045951.01.T01:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! AAAATGAAAATTAGAAATTA+GGG + Chr8:66843164-66843183 MsG0880045951.01.T01:intron 10.0%
!!! TTTTTTTTTTTTGTTTCAAA+GGG + Chr8:66843432-66843451 MsG0880045951.01.T01:intron 10.0%
!!! TTTTTTTTTTTTTGTTTCAA+AGG + Chr8:66843431-66843450 MsG0880045951.01.T01:intron 10.0%
!! AAACAAATTGATGAAATTAT+TGG - Chr8:66843410-66843429 None:intergenic 15.0%
!! CAAAATGAAAATTAGAAATT+AGG + Chr8:66843163-66843182 MsG0880045951.01.T01:intron 15.0%
!!! AATGAAAATTTACTTTTCAA+GGG - Chr8:66843619-66843638 None:intergenic 15.0%
!!! CTAATTTCTAATTTTCATTT+TGG - Chr8:66843165-66843184 None:intergenic 15.0%
!!! GATTTTTTTGTTGAAAAATT+GGG + Chr8:66843190-66843209 MsG0880045951.01.T01:intron 15.0%
!!! TGATTTTTTTGTTGAAAAAT+TGG + Chr8:66843189-66843208 MsG0880045951.01.T01:intron 15.0%
!!! AATTTTTGTTTTGTATATGC+AGG + Chr8:66843262-66843281 MsG0880045951.01.T01:intron 20.0%
!!! CAATGAAAATTTACTTTTCA+AGG - Chr8:66843620-66843639 None:intergenic 20.0%
!!! CTTGAAAAGTAAATTTTCAT+TGG + Chr8:66843618-66843637 MsG0880045951.01.T01:intron 20.0%
!!! GATTATTAACAGAAGTTTTA+AGG + Chr8:66843577-66843596 MsG0880045951.01.T01:intron 20.0%
!!! TTTCATTGGTTAAACATTTT+GGG + Chr8:66843632-66843651 MsG0880045951.01.T01:intron 20.0%
!!! TTTTCATTGGTTAAACATTT+TGG + Chr8:66843631-66843650 MsG0880045951.01.T01:intron 20.0%
! ACTTGAAATTCGTTATGTTA+GGG + Chr8:66843747-66843766 MsG0880045951.01.T01:intron 25.0%
! CAAAACCCTAATTTCTTTAT+CGG - Chr8:66843026-66843045 None:intergenic 25.0%
! CAGAACTATGATTGTAAAAT+TGG + Chr8:66843540-66843559 MsG0880045951.01.T01:intron 25.0%
! GAACAAGTTAAAGAATTTGA+GGG + Chr8:66843831-66843850 MsG0880045951.01.T01:CDS 25.0%
! TACTTGAAATTCGTTATGTT+AGG + Chr8:66843746-66843765 MsG0880045951.01.T01:intron 25.0%
! TTAATTATACGTGTTAGATC+TGG + Chr8:66843228-66843247 MsG0880045951.01.T01:intron 25.0%
! TTCTTCCGATAAAGAAATTA+GGG + Chr8:66843018-66843037 MsG0880045951.01.T01:five_prime_UTR 25.0%
!! ATAAAGAAATTAGGGTTTTG+AGG + Chr8:66843026-66843045 MsG0880045951.01.T01:five_prime_UTR 25.0%
!! TTATAGATCTGAAGTATATG+TGG + Chr8:66843342-66843361 MsG0880045951.01.T01:intron 25.0%
!!! ATTAACAGAAGTTTTAAGGA+TGG + Chr8:66843581-66843600 MsG0880045951.01.T01:intron 25.0%
!!! CTTTTTTGTTTTTTTCAAGC+TGG - Chr8:66843291-66843310 None:intergenic 25.0%
!!! TAAAGAAATTAGGGTTTTGA+GGG + Chr8:66843027-66843046 MsG0880045951.01.T01:five_prime_UTR 25.0%
!!! TATTTTGATTCCATGATGTT+TGG + Chr8:66843670-66843689 MsG0880045951.01.T01:intron 25.0%
AAAGCAATCTTGATTATACC+TGG - Chr8:66843324-66843343 None:intergenic 30.0%
ATAAAGGGAAAGGAATCTAA+TGG + Chr8:66843789-66843808 MsG0880045951.01.T01:CDS 30.0%
CGAACAAGTTAAAGAATTTG+AGG + Chr8:66843830-66843849 MsG0880045951.01.T01:CDS 30.0%
CTTCTTCCGATAAAGAAATT+AGG + Chr8:66843017-66843036 MsG0880045951.01.T01:five_prime_UTR 30.0%
TAAAGGGAAAGGAATCTAAT+GGG + Chr8:66843790-66843809 MsG0880045951.01.T01:CDS 30.0%
! TTGGCTTATGAGCATTTTAA+CGG + Chr8:66843689-66843708 MsG0880045951.01.T01:intron 30.0%
!!! TTTCATTTTGGAATTGTCGA+GGG - Chr8:66843153-66843172 None:intergenic 30.0%
!!! TTTTCATTTTGGAATTGTCG+AGG - Chr8:66843154-66843173 None:intergenic 30.0%
AATTCGTTATGTTAGGGAGT+TGG + Chr8:66843753-66843772 MsG0880045951.01.T01:intron 35.0%
ATTCGTTATGTTAGGGAGTT+GGG + Chr8:66843754-66843773 MsG0880045951.01.T01:intron 35.0%
CAAAAAAGCTATGTCAATCC+AGG + Chr8:66843303-66843322 MsG0880045951.01.T01:CDS 35.0%
GGGAGAAAATATAAAGGGAA+AGG + Chr8:66843779-66843798 MsG0880045951.01.T01:CDS 35.0%
GGGTTGGGAGAAAATATAAA+GGG + Chr8:66843774-66843793 MsG0880045951.01.T01:CDS 35.0%
TAACGGTCAAAAAGCTAAGA+TGG + Chr8:66843073-66843092 MsG0880045951.01.T01:CDS 35.0%
TGTGCGCAAAACGAAAATAA+TGG - Chr8:66842988-66843007 None:intergenic 35.0%
! TGGGTTGGGAGAAAATATAA+AGG + Chr8:66843773-66843792 MsG0880045951.01.T01:CDS 35.0%
!! ACAATCATAGTTCTGAACAG+AGG - Chr8:66843535-66843554 None:intergenic 35.0%
AAAACTGAGCCCCAAATCAA+GGG - Chr8:66843473-66843492 None:intergenic 40.0%
GTTTCAAAGGGTTCACTGTT+TGG + Chr8:66843444-66843463 MsG0880045951.01.T01:intron 40.0%
TAAAACTGAGCCCCAAATCA+AGG - Chr8:66843474-66843493 None:intergenic 40.0%
TGCTCATAAGCCAAACATCA+TGG - Chr8:66843683-66843702 None:intergenic 40.0%
TGTTTGGATCCCCTTGATTT+GGG + Chr8:66843460-66843479 MsG0880045951.01.T01:intron 40.0%
TTATGTTAGGGAGTTGGGTT+GGG + Chr8:66843759-66843778 MsG0880045951.01.T01:intron 40.0%
!! GCTTATGAGCATTTTAACGG+AGG + Chr8:66843692-66843711 MsG0880045951.01.T01:intron 40.0%
!!! TTTTGGAATTGTCGAGGGAA+TGG - Chr8:66843148-66843167 None:intergenic 40.0%
AAACTGAGCCCCAAATCAAG+GGG - Chr8:66843472-66843491 None:intergenic 45.0%
AAGCAGAAGAATGCTGGCAA+AGG + Chr8:66843116-66843135 MsG0880045951.01.T01:CDS 45.0%
CTGTTTGGATCCCCTTGATT+TGG + Chr8:66843459-66843478 MsG0880045951.01.T01:intron 45.0%
GTTATGTTAGGGAGTTGGGT+TGG + Chr8:66843758-66843777 MsG0880045951.01.T01:intron 45.0%
! GTTTGGATCCCCTTGATTTG+GGG + Chr8:66843461-66843480 MsG0880045951.01.T01:intron 45.0%
CAGCATTCTTCTGCTTCTCG+AGG - Chr8:66843112-66843131 None:intergenic 50.0%
CTCGAGAAGCAGAAGAATGC+TGG + Chr8:66843110-66843129 MsG0880045951.01.T01:CDS 50.0%
AAGCTAAGATGGCTCGCGAG+AGG + Chr8:66843084-66843103 MsG0880045951.01.T01:CDS 55.0%
TCAGCCATGGGTGGAGGTAA+CGG + Chr8:66843056-66843075 MsG0880045951.01.T01:exon 55.0%
TGAGGGTCTCTCTCAGCCAT+GGG + Chr8:66843044-66843063 MsG0880045951.01.T01:exon 55.0%
TTGACCGTTACCTCCACCCA+TGG - Chr8:66843063-66843082 None:intergenic 55.0%
TTGAGGGTCTCTCTCAGCCA+TGG + Chr8:66843043-66843062 MsG0880045951.01.T01:exon 55.0%
TCTCTCTCAGCCATGGGTGG+AGG + Chr8:66843050-66843069 MsG0880045951.01.T01:exon 60.0%
GGGTCTCTCTCAGCCATGGG+TGG + Chr8:66843047-66843066 MsG0880045951.01.T01:exon 65.0%
Chromosome Type Strat End Strand Name
Chr8 gene 66842983 66843857 66842983 ID=MsG0880045951.01;Name=MsG0880045951.01
Chr8 mRNA 66842983 66843857 66842983 ID=MsG0880045951.01.T01;Parent=MsG0880045951.01;Name=MsG0880045951.01.T01;_AED=0.48;_eAED=0.65;_QI=79|0.5|0.66|1|1|1|3|0|68
Chr8 exon 66842983 66843137 66842983 ID=MsG0880045951.01.T01:exon:13866;Parent=MsG0880045951.01.T01
Chr8 exon 66843284 66843324 66843284 ID=MsG0880045951.01.T01:exon:13867;Parent=MsG0880045951.01.T01
Chr8 exon 66843768 66843857 66843768 ID=MsG0880045951.01.T01:exon:13868;Parent=MsG0880045951.01.T01
Chr8 five_prime_UTR 66842983 66843061 66842983 ID=MsG0880045951.01.T01:five_prime_utr;Parent=MsG0880045951.01.T01
Chr8 CDS 66843062 66843137 66843062 ID=MsG0880045951.01.T01:cds;Parent=MsG0880045951.01.T01
Chr8 CDS 66843284 66843324 66843284 ID=MsG0880045951.01.T01:cds;Parent=MsG0880045951.01.T01
Chr8 CDS 66843768 66843857 66843768 ID=MsG0880045951.01.T01:cds;Parent=MsG0880045951.01.T01
Gene Sequence

>MsG0880045951.01.T01

ATGGGTGGAGGTAACGGTCAAAAAGCTAAGATGGCTCGCGAGAGGAACCTCGAGAAGCAGAAGAATGCTGGCAAAGGAAGCCAGCTTGAAAAAAACAAAAAAGCTATGTCAATCCAGGGAGTTGGGTTGGGAGAAAATATAAAGGGAAAGGAATCTAATGGGTTATGTTGTATTTTAAACGAACAAGTTAAAGAATTTGAGGGTTGA

Protein sequence

>MsG0880045951.01.T01

MGGGNGQKAKMARERNLEKQKNAGKGSQLEKNKKAMSIQGVGLGENIKGKESNGLCCILNEQVKEFEG*