Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0780039116.01.T01 | KEH23111.1 | 76.29 | 717 | 160 | 4 | 7 | 719 | 40 | 750 | 0 | 1018 |
| MsG0780039116.01.T01 | KEH23111.1 | 27.183 | 504 | 227 | 14 | 41 | 490 | 348 | 765 | 9.73E-19 | 104 |
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0780039116.01.T01 | A0A072U0Y9 | 76.290 | 717 | 160 | 4 | 7 | 719 | 40 | 750 | 0.0 | 1018 |
Co-expression Network
PPI
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0780039116.01.T01 | MTR_7g066620 | 76.290 | 717 | 160 | 4 | 7 | 719 | 40 | 750 | 0.0 | 1018 |
| MsG0780039116.01.T01 | MTR_7g066620 | 27.183 | 504 | 227 | 14 | 41 | 490 | 348 | 765 | 1.18e-22 | 104 |
| MsG0780039116.01.T01 | MTR_7g066590 | 72.126 | 696 | 159 | 5 | 50 | 719 | 1 | 687 | 0.0 | 949 |
| MsG0780039116.01.T01 | MTR_7g066590 | 26.457 | 635 | 305 | 17 | 5 | 490 | 81 | 702 | 2.13e-22 | 103 |
| MsG0780039116.01.T01 | MTR_7g066590 | 25.105 | 478 | 275 | 14 | 37 | 457 | 266 | 717 | 1.52e-14 | 78.2 |
| MsG0780039116.01.T01 | MTR_8g077105 | 36.585 | 656 | 329 | 15 | 138 | 717 | 1 | 645 | 5.60e-96 | 315 |
| MsG0780039116.01.T01 | MTR_8g077105 | 27.019 | 718 | 410 | 25 | 13 | 679 | 1 | 655 | 4.84e-30 | 127 |
| MsG0780039116.01.T01 | MTR_8g077105 | 27.404 | 416 | 241 | 12 | 363 | 734 | 1 | 399 | 3.50e-22 | 102 |
| MsG0780039116.01.T01 | MTR_8g077105 | 26.767 | 467 | 273 | 14 | 10 | 451 | 273 | 695 | 2.19e-20 | 96.7 |
| MsG0780039116.01.T01 | MTR_8g077105 | 26.898 | 461 | 280 | 14 | 8 | 437 | 223 | 657 | 2.65e-19 | 93.2 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0780039116.01.T01 | AT5G49290 | 37.250 | 749 | 407 | 20 | 5 | 719 | 55 | 774 | 1.90e-103 | 338 |
| MsG0780039116.01.T01 | AT5G49290 | 27.393 | 679 | 344 | 24 | 1 | 581 | 129 | 756 | 8.23e-24 | 108 |
| MsG0780039116.01.T01 | AT5G49290 | 26.877 | 506 | 265 | 18 | 16 | 457 | 316 | 780 | 4.81e-14 | 76.6 |
| MsG0780039116.01.T01 | AT5G49290 | 37.216 | 747 | 406 | 20 | 5 | 717 | 83 | 800 | 2.70e-103 | 338 |
| MsG0780039116.01.T01 | AT5G49290 | 27.393 | 679 | 344 | 24 | 1 | 581 | 157 | 784 | 6.22e-24 | 108 |
| MsG0780039116.01.T01 | AT5G49290 | 26.877 | 506 | 265 | 18 | 16 | 457 | 344 | 808 | 3.98e-14 | 77.0 |
| MsG0780039116.01.T01 | AT5G49290 | 36.671 | 769 | 405 | 20 | 5 | 717 | 83 | 825 | 2.39e-101 | 333 |
| MsG0780039116.01.T01 | AT5G49290 | 26.877 | 506 | 265 | 18 | 16 | 457 | 369 | 833 | 3.67e-14 | 77.0 |
| MsG0780039116.01.T01 | AT5G49290 | 36.671 | 769 | 405 | 20 | 5 | 717 | 83 | 825 | 2.39e-101 | 333 |
| MsG0780039116.01.T01 | AT5G49290 | 26.877 | 506 | 265 | 18 | 16 | 457 | 369 | 833 | 3.67e-14 | 77.0 |
Find 129 sgRNAs with CRISPR-Local
Find 292 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| AAAATCGAGAATCTTTAAGC+TGG | 0.525042 | 7:+58198154 | None:intergenic |
| AAACCATTTGGTAATGTAGT+AGG | 0.429049 | 7:+58194288 | None:intergenic |
| AAACCTCTAAGGAGCTTAAG+TGG | 0.520413 | 7:+58195464 | None:intergenic |
| AAAGGGACAATTCCTGATAA+AGG | 0.365467 | 7:-58196700 | MsG0780039116.01.T01:intron |
| AAAGTAAGATTCCTAATAGA+TGG | 0.315184 | 7:+58196739 | None:intergenic |
| AAATCCAGATATTCAAGTGA+AGG | 0.584679 | 7:+58195988 | None:intergenic |
| AAATTTGGAATACTTAGCAT+TGG | 0.500722 | 7:-58196422 | MsG0780039116.01.T01:CDS |
| AACAATTACCTCCATTTGGA+AGG | 0.482877 | 7:+58196302 | None:intergenic |
| AACGGTATCCTTCCAAATGG+AGG | 0.602865 | 7:-58196310 | MsG0780039116.01.T01:intron |
| AAGAAAGTTTGGAAGGGGAA+TGG | 0.527732 | 7:+58195786 | None:intergenic |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| AAAACCTACACACAAAAAGA+GGG | + | Chr7:58196102-58196121 | None:intergenic | 30.0% | |
| AAAATCGAGAATCTTTAAGC+TGG | + | Chr7:58194404-58194423 | None:intergenic | 30.0% | |
| AAATCCAGATATTCAAGTGA+AGG | + | Chr7:58196570-58196589 | None:intergenic | 30.0% | |
| AAATCGCTCAAAAAAAAGAG+GGG | - | Chr7:58195516-58195535 | MsG0780039116.01.T01:CDS | 30.0% | |
| AAATCGCTCAAAAAAAGAAG+AGG | + | Chr7:58194951-58194970 | None:intergenic | 30.0% | |
| AACACAATTGGTAAAAGATG+GGG | - | Chr7:58195489-58195508 | MsG0780039116.01.T01:CDS | 30.0% | |
| AAGCACTTGCAATTGAAATT+TGG | + | Chr7:58196721-58196740 | None:intergenic | 30.0% | |
| AATATGGTATCGCAGAATAT+TGG | + | Chr7:58194792-58194811 | None:intergenic | 30.0% | |
| AATCGCTCAAAAAAAGAAGA+GGG | + | Chr7:58194950-58194969 | None:intergenic | 30.0% | |
| ACAATTAGCAAAAAATAGGG+GGG | + | Chr7:58194914-58194933 | None:intergenic | 30.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| Chr7 | gene | 58194286 | 58198291 | 58194286 | ID=MsG0780039116.01;Name=MsG0780039116.01 |
| Chr7 | mRNA | 58194286 | 58198291 | 58194286 | ID=MsG0780039116.01.T01;Parent=MsG0780039116.01;Name=MsG0780039116.01.T01;_AED=0.48;_eAED=0.49;_QI=0|0.5|0.2|0.8|0.75|0.6|5|0|760 |
| Chr7 | exon | 58198288 | 58198291 | 58198288 | ID=MsG0780039116.01.T01:exon:6652;Parent=MsG0780039116.01.T01 |
| Chr7 | exon | 58198117 | 58198212 | 58198117 | ID=MsG0780039116.01.T01:exon:6651;Parent=MsG0780039116.01.T01 |
| Chr7 | exon | 58196701 | 58196850 | 58196701 | ID=MsG0780039116.01.T01:exon:6650;Parent=MsG0780039116.01.T01 |
| Chr7 | exon | 58196311 | 58196460 | 58196311 | ID=MsG0780039116.01.T01:exon:6649;Parent=MsG0780039116.01.T01 |
| Chr7 | exon | 58194286 | 58196168 | 58194286 | ID=MsG0780039116.01.T01:exon:6648;Parent=MsG0780039116.01.T01 |
| Chr7 | CDS | 58198288 | 58198291 | 58198288 | ID=MsG0780039116.01.T01:cds;Parent=MsG0780039116.01.T01 |
| Chr7 | CDS | 58198117 | 58198212 | 58198117 | ID=MsG0780039116.01.T01:cds;Parent=MsG0780039116.01.T01 |
| Chr7 | CDS | 58196701 | 58196850 | 58196701 | ID=MsG0780039116.01.T01:cds;Parent=MsG0780039116.01.T01 |
| Chr7 | CDS | 58196311 | 58196460 | 58196311 | ID=MsG0780039116.01.T01:cds;Parent=MsG0780039116.01.T01 |
| Chr7 | CDS | 58194286 | 58196168 | 58194286 | ID=MsG0780039116.01.T01:cds;Parent=MsG0780039116.01.T01 |
| Gene Sequence |
>MsG0780039116.01.T01 ATGTTCCATTATTTACGTCTTGCAGGATTGTCAAATTTAACCAGCTTAAAGATTCTCGATTTTAGTAATAATCAATTAGTCGATTTAATTGTTCGTGAAGGTTCAAAAAATTTAAGCAGATTAGATATTCTTGATTTAGATTCCAATATGATCAATGGAAGCAATCTACAAGAGTGGTTATGGGCTTTCCCATCTATTAGGAATCTTACTTTGAGATATAACCAGTTTAAAGGGACAATTCCTGATAAAGGTTTTCGTGGTTTAAGAAATTTGGAATACTTAGCATTGGATGACAGTTCTAACTTACAAAATGAGTTTTTCAAAAGTATTGGGGATTTGCCTTCTTTAGAAGTTTTGTCTGTGCTAGGATGTGGGATAAACGGTATCCTTCCAAATGGAGATTGGTCTAAGCTTAAGAAGTTGGAAGAGTTAGATCTATCAGGCAATGAATTTGTGGGGAAACTCCCCTCATCTTTTTTCAACATGACAGCACTTCTTACCTTGAAACTTGCTGATAATCATTTTATTGGTAATATTGGTCCTAACCTTGCAAGTTTTCCTTCACTTGAATATCTGGATTTTGAAGGAAACCAATTCGAATTCCCTATTTCATTCACACATTTTTCCGATCATTCAAACCTTAAGTTCATCTACGGTAATGGAAACAAAGTCATCCTTGATTCACCCTCAAATTTGAAAACATTGGTTCCCAAATTTCAATTGCAAGTGCTTCAGTTGTCATCAATAACCGAGTTCAATTCCATTCCCCTTCCAAACTTTCTTCTTTACCAACACAATTTAACTTATGTGGATTTCACTGGTTGTAAACTAAGAGGAAAGTTCCCAAATTGGTTGTTAGAAAACAACACAAAAATGGAAAATCTTATTTTTCAGAATTGTTCCTTTGTAGGAAATTTCCAGCTTCCATCTCGTCCTCTTCTCAACTTGGCAACAATTGACGTATCTTACAATGCAATAACAGGTCAAATGTTGAGTAATAATATCAGTTCAATTTTTCCTAATTTGGTTAATTTAACCATGTCTAGAAATGCAATTCACGGTTCAATTCCTCATGACCTAAGCCACTTAAGCTCCTTAGAGGTTTTGGACCTGTCTGGTAATGAATTGTCTGGAGAAATACCAAATAACTTATCTGGATATGGGAGTCAACTCGTGCATTTGAGATATTTTTTGTTGGGTGGAAATAGGCTTTCTGGTAGCATCCCAAGTAACCTTTTTAACTTACATAGTATCGAAGGTTTGGACATAAGTAATAATAATTTCACCGGAAAAATTCCAAACCAAATAAAAAATAGTTCAAGCTTGATTGAACTTTCCATGTCCAATAATCATTTTGAAGGGTCAATTCCACCAGAGGTTGCAGAAATTAAAAGTCTAGCATTTTTAGATCTTTCACAAAATAATTTGTCAGGTTGTGTTCCTTCTTTTGTAAACAACTCTGCAACCTTTATCCATTTGAGCAACAACAAATTAAGTTGTTTATCCAAAAACATGTCCGGTGGGAACATAGATTTGTCTTCTTACTTAGTGACTCTAGACCTTAGCAACAATGAAATATCAAATGGAATTCATGATCTAATACATGACCTCCGTTATACCAGCTTGAAATTTCTCCTTATGAAAGGTAATAACTTCACAGGGAATATACCAAAGCAATTATGCCACCTTACTGATTTAGATATACTAGACCTTTCTTACAATAATTTTATTGGGGAAATACCTAGTTGTTTAGGTAAAATGATTTTTGAGAATGAGAATCGAGATGCATCAATATTCAATGGAGTCGTTTCTGGTAGTGTAGACTATATATATAGCAGATTTGGGAAAGAGAGAGCAAACTTTACATCAAAGAAAAGATTAGAAACTTATACAACAAATATTCTTATTTATATGTCTGGAATTGATTTATCTCATAATAAGTTAAATGGTAGCATCCCATCTGAGCTAGGAAACTTGACAAAAATTCGAGCTTTGAACTTGTCAAACAATTTTTTGACTGGGAAAGTTCCAACTACATTCTCCAATTTGATACAAGTTGAGAGTTTAGATCTTTCTTTCAACATGTTGAGTGGCCAAATTCCTCCTCAGCTAAGTGGATTGCACTACCTTGAAGTATTCATTGTTCAACACCTGAATGGAAAGGACAATTTAGTACATTTGATGAAAGCAGCTATGAAGGTAATCAATTTCTTTGTGGACTTCCATTGCCAAAAAGCTGCAATCCGAGTGGAGAAGCACCTACTACATTACCAAATGGTTTGA |
| Protein sequence |
>MsG0780039116.01.T01 MFHYLRLAGLSNLTSLKILDFSNNQLVDLIVREGSKNLSRLDILDLDSNMINGSNLQEWLWAFPSIRNLTLRYNQFKGTIPDKGFRGLRNLEYLALDDSSNLQNEFFKSIGDLPSLEVLSVLGCGINGILPNGDWSKLKKLEELDLSGNEFVGKLPSSFFNMTALLTLKLADNHFIGNIGPNLASFPSLEYLDFEGNQFEFPISFTHFSDHSNLKFIYGNGNKVILDSPSNLKTLVPKFQLQVLQLSSITEFNSIPLPNFLLYQHNLTYVDFTGCKLRGKFPNWLLENNTKMENLIFQNCSFVGNFQLPSRPLLNLATIDVSYNAITGQMLSNNISSIFPNLVNLTMSRNAIHGSIPHDLSHLSSLEVLDLSGNELSGEIPNNLSGYGSQLVHLRYFLLGGNRLSGSIPSNLFNLHSIEGLDISNNNFTGKIPNQIKNSSSLIELSMSNNHFEGSIPPEVAEIKSLAFLDLSQNNLSGCVPSFVNNSATFIHLSNNKLSCLSKNMSGGNIDLSSYLVTLDLSNNEISNGIHDLIHDLRYTSLKFLLMKGNNFTGNIPKQLCHLTDLDILDLSYNNFIGEIPSCLGKMIFENENRDASIFNGVVSGSVDYIYSRFGKERANFTSKKRLETYTTNILIYMSGIDLSHNKLNGSIPSELGNLTKIRALNLSNNFLTGKVPTTFSNLIQVESLDLSFNMLSGQIPPQLSGLHYLEVFIVQHLNGKDNLVHLMKAAMKVINFFVDFHCQKAAIRVEKHLLHYQMV* |
