AlfalfaGEDB | MsG0580029944.01

M. sativa cultivar ZhongmuNo.1 / MsG0580029944.01

KEGG: -
GO: GO:0005515
PFAM: PF00560

Nr:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MsG0580029944.01.T01	AET00742.2	76.649	561	71	3	1	549	193	705	0	774

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Swissprot:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MsG0580029944.01.T01	Q9M9X0	36.394	599	306	12	20	549	121	713	1.03E-76	266

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Trembl:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MsG0580029944.01.T01	G7K4M6	76.649	561	71	3	1	549	193	705	0.0	774

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TFs/TRs:

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Gene ID	Type	Classification
No data available in table

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Protein Kinases:

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Gene ID	Type	Classification
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Network:

Co-expression Network

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Gene1	Gene2	correlation coefficient	p_value	FDR
No data available in table

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PPI

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Gene1	Gene2	Type
MsG0180004711.01	MsG0580029944.01	PPI
MsG0580028439.01	MsG0580029944.01	PPI
MsG0580029012.01	MsG0580029944.01	PPI
MsG0580029013.01	MsG0580029944.01	PPI
MsG0580029480.01	MsG0580029944.01	PPI
MsG0580029936.01	MsG0580029944.01	PPI
MsG0580029936.01	MsG0580029944.01	PPI
MsG0580029936.01	MsG0580029944.01	PPI
MsG0580029944.01	MsG0780039135.01	PPI
MsG0580029944.01	MsG0080048826.01	PPI

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Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MsG0580029944.01.T01	MTR_5g095420	76.649	561	71	3	1	549	193	705	0.0	774
MsG0580029944.01.T01	MTR_2g032560	60.461	607	172	6	1	548	227	824	0.0	627
MsG0580029944.01.T01	MTR_2g032560	33.515	367	205	8	60	411	556	898	1.61e-28	122
MsG0580029944.01.T01	MTR_2g032560	27.597	616	279	22	9	479	306	899	7.33e-25	110
MsG0580029944.01.T01	MTR_2g032560	30.195	563	316	22	37	548	86	622	3.21e-21	99.4
MsG0580029944.01.T01	MTR_5g079980	59.242	633	168	6	1	549	246	872	0.0	604
MsG0580029944.01.T01	MTR_5g079980	29.663	563	321	16	17	550	214	730	5.11e-29	124
MsG0580029944.01.T01	MTR_5g079980	29.966	594	311	22	15	556	77	617	8.50e-23	104
MsG0580029944.01.T01	MTR_5g079980	27.458	590	265	17	7	444	347	925	8.50e-23	104
MsG0580029944.01.T01	MTR_5g080000	62.957	575	164	4	18	549	210	778	0.0	592

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Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MsG0580029944.01.T01	AT3G05650	36.394	599	306	12	20	549	121	713	1.05e-77	266
MsG0580029944.01.T01	AT3G05650	28.824	510	286	19	9	452	252	750	7.95e-23	104
MsG0580029944.01.T01	AT3G05650	26.590	346	203	10	257	556	117	457	1.50e-13	74.7
MsG0580029944.01.T01	AT3G05650	31.013	158	88	3	19	155	608	765	8.82e-12	68.9
MsG0580029944.01.T01	AT1G71400	36.242	596	315	12	4	551	119	697	1.30e-76	263
MsG0580029944.01.T01	AT1G71400	30.812	542	280	10	7	454	170	710	8.49e-44	169
MsG0580029944.01.T01	AT1G71400	29.399	449	241	14	19	419	327	747	1.34e-30	129
MsG0580029944.01.T01	AT1G71400	28.306	431	228	12	15	395	348	747	1.57e-28	122
MsG0580029944.01.T01	AT1G71400	27.778	360	214	11	203	559	105	421	4.27e-13	73.2
MsG0580029944.01.T01	AT2G15080	35.390	616	317	12	9	549	222	831	7.23e-76	263

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Find 128 sgRNAs with CRISPR-Local

Find 270 sgRNAs with CRISPR-GE

CRISPR-Local

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CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
AAAACAAATAAGTTAGCTTT+TGG	0.250292	5:-104337879	None:intergenic
AACATTCAGGTCCATGCAGT+TGG	0.493351	5:-104338736	None:intergenic
AAGACAAAGGAATTGGTCCT+TGG	0.503386	5:-104337558	None:intergenic
AAGATGAGAATGCTTCCAAG+TGG	0.688132	5:+104341096	MsG0580029944.01.T01:CDS
AAGCAAGTCGAGTTGAAATC+GGG	0.446196	5:+104341161	MsG0580029944.01.T01:CDS
AAGGGAAACTATTGTAACTA+TGG	0.384073	5:+104341067	MsG0580029944.01.T01:CDS
AATCGGGGTTGCGGATAAAT+TGG	0.261467	5:+104341177	MsG0580029944.01.T01:CDS
AATCTCATCATATTCCTTAG+AGG	0.334284	5:+104338008	MsG0580029944.01.T01:CDS
AATTTGTGAGATGCTTTGTA+AGG	0.406521	5:-104341239	None:intergenic
ACAAATTTCTATGTTACCGC+CGG	0.561073	5:+104341255	MsG0580029944.01.T01:CDS

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CRISPR-GE

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sgRNA_sequence	Strand	Position	Region	GC_content
AAACACAAAGCAAGTGATTA+AGG	+	Chr5:104339897-104339916	MsG0580029944.01.T01:intron	30.0%
AAGGGAAACTATTGTAACTA+TGG	+	Chr5:104341067-104341086	MsG0580029944.01.T01:CDS	30.0%
AATCTCATCATATTCCTTAG+AGG	+	Chr5:104338008-104338027	MsG0580029944.01.T01:CDS	30.0%
AATTTGTGAGATGCTTTGTA+AGG	-	Chr5:104341242-104341261	None:intergenic	30.0%
AGAAATACGATGTATTTACG+AGG	+	Chr5:104340370-104340389	MsG0580029944.01.T01:intron	30.0%
ATGTACACGTGAGTATATAT+AGG	-	Chr5:104340645-104340664	None:intergenic	30.0%
ATTTGCAATATGAGTTCACT+AGG	+	Chr5:104338516-104338535	MsG0580029944.01.T01:CDS	30.0%
ATTTGTGAGATGCTTTGTAA+GGG	-	Chr5:104341241-104341260	None:intergenic	30.0%
CAAAGAGATTCAAGATGATT+TGG	+	Chr5:104340140-104340159	MsG0580029944.01.T01:intron	30.0%
CTAAAAATGATGAGATCATC+CGG	+	Chr5:104338375-104338394	MsG0580029944.01.T01:CDS	30.0%

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Chromosome	Type	Strat	End	Strand	Name
Chr5	gene	104337469	104341301	104337469	ID=MsG0580029944.01;Name=MsG0580029944.01
Chr5	mRNA	104337469	104341301	104337469	ID=MsG0580029944.01.T01;Parent=MsG0580029944.01;Name=MsG0580029944.01.T01;_AED=0.49;_eAED=0.59;_QI=0\|0\|0\|1\|1\|1\|3\|0\|681
Chr5	exon	104337469	104338829	104337469	ID=MsG0580029944.01.T01:exon:7078;Parent=MsG0580029944.01.T01
Chr5	exon	104338937	104339223	104338937	ID=MsG0580029944.01.T01:exon:7079;Parent=MsG0580029944.01.T01
Chr5	exon	104340904	104341301	104340904	ID=MsG0580029944.01.T01:exon:7080;Parent=MsG0580029944.01.T01
Chr5	CDS	104337469	104338829	104337469	ID=MsG0580029944.01.T01:cds;Parent=MsG0580029944.01.T01
Chr5	CDS	104338937	104339223	104338937	ID=MsG0580029944.01.T01:cds;Parent=MsG0580029944.01.T01
Chr5	CDS	104340904	104341301	104340904	ID=MsG0580029944.01.T01:cds;Parent=MsG0580029944.01.T01

Gene Sequence

>MsG0580029944.01.T01

ATGTCACAAAATTTCAACCTTCAAGGTAAGCTTCCTGAACTGAGTTGTAGTGCTTCTCTCAGTAACTTGCATCTCTCAAACTGTCAATTCCAAGGACCAATTCCTTTGTCTTTTTTGAACCTCACACATCTTACTTCCTTGATTCTATCCTATAACAATCTTAACAATTCAATCCCATCCTCACTTTTCAAGCTTCGACGTCTCATTCACTTGGATCTTTCATTCAATTCATTAAGCGGTCAAATCCCATATGTGTTTGGTGGAATTACCAAATTGCAAGAACTTAATCTAGCTTCTAACAAATTAGAAGGACAAATTCCATCATCATTATTTAACTTGACTCAACTTGTTACATTTGATTGTTCTTATAATAAATTAGAGGGTCCTCTACCCAACAAAATTACAGGGTTCCAAAAGCTAACTTATTTGTTTTTAAATGGCAACTTGTTAAATGGAACAATTCCTCCATCCTTGTTTTCCTTTCCTTCCTTGGTAGAGTTAGATCTATCAAATAACCGACTTACAGTGCATATCATTGCAATCTCATCATATTCCTTAGAGGAGCTATATCTATCTAGCAACAAACTAGAAGGCAATATTCTAGAATCAATTTTCAAACTTATAAACCTAACTAGACTAGACCTATCATCAAACAACTTCAGTGGTGTTGTTGATTTTCAACACTTCTCCAAACTTCAAAATCTTGTATCTCTGTCCCTTTCACATTATAGTCAATTGTCACTAAACTTTGAATCCAATGTCAATTACAATTTTTCTCAACTAACAGAATTGGATCTGTCTTCTTTGAGTTTGACTGGATTTCAAAAATTATCAAGAAAACTTTCAAATTTGATGTATTTTGATCTGTCCAATAACAAAATTAATGGAAGAGTGCCAAATTGGTCACTAAAAATGATGAGATCATCCGGATTTTTGAATCTCTCTCAAAATTTGTTCACGTCAATAGAAGAAATCTCAAGGACTAATTATCAACTCGGTGGACTTGATCTAAGTTATAACTTACTACATGGTGAAATCTTTGTGTCAATTTGCAATATGAGTTCACTAGGATTTCTCAAATTGGCAAACAACAAATTGACAGGTATCATTCCACAATGTCTTGCTAATTTATCATACCTTAAAGTTTTGGATCTACAAATGAACAAATTTTATGGCACTTTGCCAAGTAACTTTTCAAAGGACAGTGAGCTTCATACTCTAAATCTCTATGGCAACCAATTAGAAGGCCATTTGCCTAATTCTTTGTCCAACTGCATGGACCTGAATGTTCTAAATCTTGGCAACAATAAAATAGAAGGCAATTTTCCTGAGTGGCTTCCAACTCTGTCTCATTTGAAAGTTGGTCCGCTACCAAAAGCCTATTTAAAAAATTATCAAACCATGAAGAATGTTACTGAAGCAGAAGAGGATGGATTTTATCAATATATGGAAATAGATATAGTGGGTCGACAGTACTATGATTATGTAACTGTGGCGACAAAAGGAAACAAAACCCCACTTGTGAAAATTCCAAAAATCTTTGCAAGTATTGATTTTTCAAGAAATAAATTTGATGGGGAGATTCCAAATGTTATTGGAGAGCCTCATGATCTCAAAGGGCTTAACCTTTCATATAACAAACTCACTGAAATAGAGAAAACTGCTAGGTTACTTAAGAAGCAAGCAAAGGAAGCTAAGAGATTAGCCAGGCTGGCTCAGCCGGGATCATCTAGTTCTATTCACTCCTTCGGGTTCGGTTCAGACACTACTCCGGGTGGTTCTCTACACACAACTCCACCATCATCACCGGAAAGGGAAACTATTGTAACTATGGCTAATGAAGATGAGAATGCTTCCAAGTGGGTTAGTCCAAGAAGGCTTGCTAACCTTGGTAATCAAAATGCCAAGCAAGTCGAGTTGAAATCGGGGTTGCGGATAAATTGGATCACTCAGAAATCATTCACTGGAATGGATCATGAAGACCCTTACAAAGCATCTCACAAATTTCTATGTTACCGCCGGTACTCTTGGTATGAAGGAAGATGA

Protein sequence

>MsG0580029944.01.T01

MSQNFNLQGKLPELSCSASLSNLHLSNCQFQGPIPLSFLNLTHLTSLILSYNNLNNSIPSSLFKLRRLIHLDLSFNSLSGQIPYVFGGITKLQELNLASNKLEGQIPSSLFNLTQLVTFDCSYNKLEGPLPNKITGFQKLTYLFLNGNLLNGTIPPSLFSFPSLVELDLSNNRLTVHIIAISSYSLEELYLSSNKLEGNILESIFKLINLTRLDLSSNNFSGVVDFQHFSKLQNLVSLSLSHYSQLSLNFESNVNYNFSQLTELDLSSLSLTGFQKLSRKLSNLMYFDLSNNKINGRVPNWSLKMMRSSGFLNLSQNLFTSIEEISRTNYQLGGLDLSYNLLHGEIFVSICNMSSLGFLKLANNKLTGIIPQCLANLSYLKVLDLQMNKFYGTLPSNFSKDSELHTLNLYGNQLEGHLPNSLSNCMDLNVLNLGNNKIEGNFPEWLPTLSHLKVGPLPKAYLKNYQTMKNVTEAEEDGFYQYMEIDIVGRQYYDYVTVATKGNKTPLVKIPKIFASIDFSRNKFDGEIPNVIGEPHDLKGLNLSYNKLTEIEKTARLLKKQAKEAKRLARLAQPGSSSSIHSFGFGSDTTPGGSLHTTPPSSPERETIVTMANEDENASKWVSPRRLANLGNQNAKQVELKSGLRINWITQKSFTGMDHEDPYKASHKFLCYRRYSWYEGR*