AlfalfaGEDB | MsG0780039677.01

M. sativa cultivar ZhongmuNo.1 / MsG0780039677.01

KEGG: -
GO: GO:0004867 GO:0005576
PFAM: PF00228

Nr:

Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MsG0780039677.01.T01	AES80148.2	89.524	105	9	2	1	105	5	107	1.97E-48	160

Swissprot:

Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MsG0780039677.01.T01	Q8W4Y8	50.476	105	46	2	1	103	10	110	1.04E-29	105

Trembl:

Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MsG0780039677.01.T01	G7L3M8	89.524	105	9	2	1	105	5	107	9.42e-49	160

TFs/TRs:

Gene ID	Type	Classification

Protein Kinases:

Gene ID	Type	Classification

Network:

Co-expression Network

Gene1	Gene2	correlation coefficient	p_value	FDR
MsG0180001800.01	MsG0780039677.01	0.805687	1.183459e-49	6.301677e-47
MsG0180003237.01	MsG0780039677.01	0.813499	2.481139e-51	1.623061e-48
MsG0280011267.01	MsG0780039677.01	0.818610	1.791692e-52	1.347461e-49
MsG0580025550.01	MsG0780039677.01	0.800465	1.423380e-48	6.631889e-46
MsG0580025961.01	MsG0780039677.01	0.845231	4.619001e-59	7.646362e-56
MsG0380015659.01	MsG0780039677.01	0.800001	1.768870e-48	8.145276e-46
MsG0380018070.01	MsG0780039677.01	0.811085	8.352077e-51	5.122175e-48
MsG0580030135.01	MsG0780039677.01	0.811701	6.137436e-51	3.825389e-48
MsG0780036426.01	MsG0780039677.01	0.800702	1.273143e-48	5.967694e-46
MsG0780036472.01	MsG0780039677.01	0.804758	1.852151e-49	9.629498e-47
MsG0780036649.01	MsG0780039677.01	0.814255	1.691412e-51	1.129300e-48
MsG0780039677.01	MsG0780040615.01	0.805633	1.215065e-49	6.460658e-47

PPI

Gene1	Gene2	Type

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MsG0780039677.01.T01	MTR_7g077160	89.524	105	9	2	1	105	5	107	2.39e-52	160
MsG0780039677.01.T01	MTR_7g077340	55.455	110	43	2	1	105	12	120	4.40e-37	122
MsG0780039677.01.T01	MTR_7g077215	58.163	98	37	2	3	97	14	110	1.99e-32	109
MsG0780039677.01.T01	MTR_7g077310	55.769	104	38	2	1	97	1	103	8.87e-32	107
MsG0780039677.01.T01	MTR_7g077210	55.140	107	35	4	1	97	13	116	1.49e-30	105
MsG0780039677.01.T01	MTR_7g077190	76.271	59	14	0	43	101	25	83	1.07e-27	97.1
MsG0780039677.01.T01	MTR_7g077300	57.143	98	38	3	1	97	8	102	1.21e-27	97.8
MsG0780039677.01.T01	MTR_7g077180	49.485	97	30	1	1	97	9	86	1.11e-24	89.4
MsG0780039677.01.T01	MTR_7g077260	45.455	99	47	2	6	97	15	113	1.75e-20	79.7
MsG0780039677.01.T01	MTR_7g077220	46.875	96	33	3	1	91	2	84	2.96e-19	77.4
MsG0780039677.01.T01	MTR_7g077313	40.625	96	52	3	1	94	10	102	2.06e-13	61.2

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score

Find 19 sgRNAs with CRISPR-Local

Find 19 sgRNAs with CRISPR-GE

CRISPR-Local

CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
TTCAAAACGAGCATCAATAA+TGG	0.364997	7:+67449690	None:intergenic
ACTCAGCTTGCAAGACTTGT+AGG	0.371610	7:-67449534	MsG0780039677.01.T01:CDS
ACTTGAGTGATGAGGGAGTT+TGG	0.371670	7:+67449667	None:intergenic
TGTTGTCGATTTGACACCAT+TGG	0.451422	7:+67449639	None:intergenic
AGCAAAAGTCAGTGATATCA+AGG	0.473824	7:+67449479	None:intergenic
TCATTAATCTTGATGAGCCT+TGG	0.513113	7:+67449429	None:intergenic
GTTATAGTAGTTGAGTTACA+TGG	0.521465	7:+67449451	None:intergenic
ACGAGCATCAATAATGGTTG+TGG	0.542447	7:+67449696	None:intergenic
GGATATCACTTGAGTGATGA+GGG	0.569030	7:+67449660	None:intergenic
TAACTCAACTACTATAACCA+AGG	0.583203	7:-67449446	MsG0780039677.01.T01:CDS
TCAAGGCAATCACACTTCAA+AGG	0.590156	7:+67449496	None:intergenic
ATCACTCAAGTGATATCCAA+TGG	0.590793	7:-67449655	MsG0780039677.01.T01:CDS
TTGCAAGCTGAGTGACATGT+TGG	0.596983	7:+67449544	None:intergenic
TGGATATCACTTGAGTGATG+AGG	0.599239	7:+67449659	None:intergenic
CAACCACAATTATCACAACA+TGG	0.626023	7:+67449610	None:intergenic
TCAGTACAACGACACTGAGG+AGG	0.627030	7:+67449574	None:intergenic
CAGTACAACGACACTGAGGA+GGG	0.637399	7:+67449575	None:intergenic
GCACCATGTTGTGATAATTG+TGG	0.665913	7:-67449613	MsG0780039677.01.T01:CDS
ACATCAGTACAACGACACTG+AGG	0.777519	7:+67449571	None:intergenic

CRISPR-GE

badsite warning	sgRNA_sequence	Strand	Position	Region	GC_content
	GTTATAGTAGTTGAGTTACA+TGG	+	Chr7:67449717-67449736	None:intergenic	30.0%
	TAACTCAACTACTATAACCA+AGG	-	Chr7:67449719-67449738	MsG0780039677.01.T01:CDS	30.0%
	TTCAAAACGAGCATCAATAA+TGG	+	Chr7:67449478-67449497	None:intergenic	30.0%
!!	GCTTTGTTACTTGTTTTCTT+AGG	-	Chr7:67449441-67449460	MsG0780039677.01.T01:CDS	30.0%
	AGCAAAAGTCAGTGATATCA+AGG	+	Chr7:67449689-67449708	None:intergenic	35.0%
	ATCACTCAAGTGATATCCAA+TGG	-	Chr7:67449510-67449529	MsG0780039677.01.T01:CDS	35.0%
	CAACCACAATTATCACAACA+TGG	+	Chr7:67449558-67449577	None:intergenic	35.0%
	ACGAGCATCAATAATGGTTG+TGG	+	Chr7:67449472-67449491	None:intergenic	40.0%
	GCACCATGTTGTGATAATTG+TGG	-	Chr7:67449552-67449571	MsG0780039677.01.T01:CDS	40.0%
	GGATATCACTTGAGTGATGA+GGG	+	Chr7:67449508-67449527	None:intergenic	40.0%
	TCAAGGCAATCACACTTCAA+AGG	+	Chr7:67449672-67449691	None:intergenic	40.0%
	TGGATATCACTTGAGTGATG+AGG	+	Chr7:67449509-67449528	None:intergenic	40.0%
!!	TGTTGTCGATTTGACACCAT+TGG	+	Chr7:67449529-67449548	None:intergenic	40.0%
	ACATCAGTACAACGACACTG+AGG	+	Chr7:67449597-67449616	None:intergenic	45.0%
	ACTCAGCTTGCAAGACTTGT+AGG	-	Chr7:67449631-67449650	MsG0780039677.01.T01:CDS	45.0%
	ACTTGAGTGATGAGGGAGTT+TGG	+	Chr7:67449501-67449520	None:intergenic	45.0%
	TTGCAAGCTGAGTGACATGT+TGG	+	Chr7:67449624-67449643	None:intergenic	45.0%
	CAGTACAACGACACTGAGGA+GGG	+	Chr7:67449593-67449612	None:intergenic	50.0%
	TCAGTACAACGACACTGAGG+AGG	+	Chr7:67449594-67449613	None:intergenic	50.0%

Chromosome	Type	Strat	End	Strand	Name
Chr7	gene	67449432	67449755	67449432	ID=MsG0780039677.01;Name=MsG0780039677.01
Chr7	mRNA	67449432	67449755	67449432	ID=MsG0780039677.01.T01;Parent=MsG0780039677.01;Name=MsG0780039677.01.T01;_AED=0.35;_eAED=0.36;_QI=0\|-1\|0\|1\|-1\|1\|1\|0\|107
Chr7	exon	67449432	67449755	67449432	ID=MsG0780039677.01.T01:exon:11344;Parent=MsG0780039677.01.T01
Chr7	CDS	67449432	67449755	67449432	ID=MsG0780039677.01.T01:cds;Parent=MsG0780039677.01.T01
Chr7	mRNA	67449512	67449658	67449512	ID=MsG0780039677.01.T02;Parent=MsG0780039677.01;Name=MsG0780039677.01.T02;_AED=0.43;_eAED=1.00;_QI=0\|-1\|0\|1\|-1\|0\|1\|0\|48
Chr7	exon	67449512	67449658	67449512	ID=MsG0780039677.01.T02:exon:11345;Parent=MsG0780039677.01.T02
Chr7	CDS	67449512	67449658	67449512	ID=MsG0780039677.01.T02:cds;Parent=MsG0780039677.01.T02
Chr7	mRNA	67449512	67449574	67449512	ID=MsG0780039677.01.T03;Parent=MsG0780039677.01;Name=MsG0780039677.01.T03;_AED=0.47;_eAED=1.00;_QI=0\|-1\|0\|1\|-1\|0\|1\|0\|20
Chr7	exon	67449512	67449574	67449512	ID=MsG0780039677.01.T03:exon:11346;Parent=MsG0780039677.01.T03
Chr7	CDS	67449512	67449574	67449512	ID=MsG0780039677.01.T03:cds;Parent=MsG0780039677.01.T03

Gene Sequence

>MsG0780039677.01.T01

ATGAAGATAGCTTTGTTACTTGTTTTCTTAGGTTTGACCACAACCATTATTGATGCTCGTTTTGAACCAAACTCCCTCATCACTCAAGTGATATCCAATGGTGTCAAATCGACAACATGTGCACCATGTTGTGATAATTGTGGTTGCACAAAATCAATCCCTCCTCAGTGTCGTTGTACTGATGTTAAACCAACATGTCACTCAGCTTGCAAGACTTGTAGGTGTTTTGAGACATTACCTTTGAAGTGTGATTGCCTTGATATCACTGACTTTTGCTATGAACCATGTAACTCAACTACTATAACCAAGGCTCATCAAGATTAA

>MsG0780039677.01.T02

ATGGTGTCAAATCGACAACATGTGCACCATGTTGTGATAATTGTGGTTGCACAAAATCAATCCCTCCTCAGTGTCGTTGTACTGATGTTAAACCAACATGTCACTCAGCTTGCAAGACTTGTAGGTGTTTTGAGACATTACCTTTGA

>MsG0780039677.01.T03

ATGTTAAACCAACATGTCACTCAGCTTGCAAGACTTGTAGGTGTTTTGAGACATTACCTTTGA

Protein sequence

>MsG0780039677.01.T01

MKIALLLVFLGLTTTIIDARFEPNSLITQVISNGVKSTTCAPCCDNCGCTKSIPPQCRCTDVKPTCHSACKTCRCFETLPLKCDCLDITDFCYEPCNSTTITKAHQD*

>MsG0780039677.01.T02

MVSNRQHVHHVVIIVVAQNQSLLSVVVLMLNQHVTQLARLVGVLRHYL*

>MsG0780039677.01.T03

MLNQHVTQLARLVGVLRHYL*