AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar ZhongmuNo.1 / MsG0180003237.01


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsG0180003237.01.T01 MTR_1g056370 92.398 171 13 0 1 171 16 186 7.60e-118 331
MsG0180003237.01.T01 MTR_4g078885 49.375 160 76 3 11 170 30 184 4.54e-49 157
MsG0180003237.01.T01 MTR_4g013330 48.026 152 75 2 23 171 49 199 1.62e-48 156
MsG0180003237.01.T01 MTR_3g034030 48.125 160 78 3 11 170 30 184 1.67e-47 153
MsG0180003237.01.T01 MTR_4g013320 47.368 152 74 3 23 171 51 199 1.79e-47 153
MsG0180003237.01.T01 MTR_4g013350 47.333 150 77 2 23 171 49 197 2.40e-46 150
MsG0180003237.01.T01 MTR_4g013325 45.890 146 77 2 27 171 55 199 7.23e-46 149
MsG0180003237.01.T01 MTR_4g013345 46.000 150 79 2 23 171 48 196 3.17e-45 147
MsG0180003237.01.T01 MTR_7g093820 47.682 151 76 3 23 171 45 194 6.33e-45 147
MsG0180003237.01.T01 MTR_7g093850 48.344 151 75 3 23 171 45 194 7.21e-45 146
MsG0180003237.01.T01 MTR_7g093830 48.344 151 75 3 23 171 44 193 9.19e-45 146
MsG0180003237.01.T01 MTR_7g093790 45.033 151 80 3 23 171 42 191 2.66e-44 145
MsG0180003237.01.T01 MTR_1g054525 46.667 150 76 3 23 171 46 192 3.26e-43 142
MsG0180003237.01.T01 MTR_4g013335 43.624 149 82 2 23 171 50 196 6.72e-43 141
MsG0180003237.01.T01 MTR_4g013385 44.295 149 82 1 23 171 43 190 3.42e-42 139
MsG0180003237.01.T01 MTR_0433s0040 44.295 149 82 1 23 171 43 190 3.42e-42 139
MsG0180003237.01.T01 MTR_7g070390 43.976 166 89 2 6 171 31 192 6.97e-42 139
MsG0180003237.01.T01 MTR_4g013770 45.578 147 77 3 26 170 45 190 4.32e-41 137
MsG0180003237.01.T01 MTR_4g073950 41.892 148 83 2 26 171 46 192 1.20e-40 135
MsG0180003237.01.T01 MTR_4g013310 42.763 152 83 3 21 171 49 197 2.08e-40 135
MsG0180003237.01.T01 MTR_3g105630 43.421 152 83 1 23 171 38 189 5.71e-39 131
MsG0180003237.01.T01 MTR_3g105640 41.558 154 82 2 23 171 38 188 6.32e-39 131
MsG0180003237.01.T01 MTR_7g093870 42.208 154 84 2 23 171 40 193 1.91e-37 128
MsG0180003237.01.T01 MTR_5g096120 36.250 160 96 2 17 171 31 189 6.99e-35 121
MsG0180003237.01.T01 MTR_4g013355 38.667 150 80 3 23 171 41 179 2.10e-29 106
MsG0180003237.01.T01 MTR_4g122110 37.415 147 90 2 26 171 35 180 2.43e-25 96.3
MsG0180003237.01.T01 MTR_4g122130 35.811 148 91 3 26 171 35 180 4.99e-22 87.8
MsG0180003237.01.T01 MTR_4g049550 30.612 147 100 2 23 168 30 175 7.93e-20 82.0
MsG0180003237.01.T01 MTR_4g049570 32.394 142 94 2 28 168 35 175 1.76e-18 78.6
MsG0180003237.01.T01 MTR_8g099115 34.091 132 76 4 28 150 39 168 1.25e-14 68.6
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsG0180003237.01.T01 AT1G65870 48.701 154 77 2 18 170 37 189 3.72e-49 157
MsG0180003237.01.T01 AT5G42500 46.746 169 81 4 8 170 20 185 3.70e-46 149
MsG0180003237.01.T01 AT3G13650 43.558 163 86 4 9 171 30 186 6.01e-46 149
MsG0180003237.01.T01 AT5G42510 48.322 149 73 3 23 170 37 182 1.81e-45 147
MsG0180003237.01.T01 AT1G22900 50.340 147 69 3 25 170 50 193 8.27e-45 146
MsG0180003237.01.T01 AT1G58170 42.515 167 85 3 15 171 20 185 6.08e-44 144
MsG0180003237.01.T01 AT1G55210 46.309 149 77 2 23 171 42 187 1.85e-43 142
MsG0180003237.01.T01 AT1G55210 46.309 149 77 2 23 171 42 187 1.85e-43 142
MsG0180003237.01.T01 AT2G21100 43.151 146 81 2 26 170 43 187 3.52e-41 137
MsG0180003237.01.T01 AT2G21100 43.151 146 81 2 26 170 43 187 3.52e-41 137
MsG0180003237.01.T01 AT5G49040 39.634 164 95 3 9 171 31 191 1.78e-40 135
MsG0180003237.01.T01 AT3G13662 43.382 136 74 2 23 158 40 172 7.53e-36 123
MsG0180003237.01.T01 AT3G58090 47.101 138 63 3 38 170 139 271 9.57e-34 120
MsG0180003237.01.T01 AT2G21110 40.397 151 84 2 26 171 37 186 2.08e-33 117
MsG0180003237.01.T01 AT5G49030 41.259 143 80 3 9 150 31 170 4.94e-32 121
MsG0180003237.01.T01 AT3G13660 39.683 126 75 1 46 171 1 125 1.06e-31 110
MsG0180003237.01.T01 AT4G38700 35.669 157 95 3 21 171 34 190 7.23e-28 103
MsG0180003237.01.T01 AT4G23690 37.903 124 70 5 27 146 42 162 1.83e-17 76.3
MsG0180003237.01.T01 AT1G64160 33.898 118 71 4 31 144 41 155 3.89e-15 69.7
MsG0180003237.01.T01 AT4G11190 33.884 121 73 5 30 146 41 158 6.42e-14 66.6
MsG0180003237.01.T01 AT4G11210 31.081 148 99 3 3 150 18 162 1.19e-13 65.9

Find 43 sgRNAs with CRISPR-Local

Find 47 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
ATAAGCTCTGAACTTAAATT+TGG 0.164549 1:+58821462 None:intergenic
AAATTTGGTTCTTTAGTTAA+AGG 0.202826 1:+58821477 None:intergenic
ATGCTTTGGCTAAGACTATT+TGG 0.206020 1:-58821242 MsG0180003237.01.T01:CDS
TTTCTGAGGGTAAACTTATA+TGG 0.219973 1:+58821637 None:intergenic
GAATCATCTCATGGTCCTTT+TGG 0.240305 1:-58821522 MsG0180003237.01.T01:CDS
GGTGAGGCTGCTCCCTTTGT+AGG 0.246807 1:+58821350 None:intergenic
GCTTGGGAGGAATGTTATTA+TGG 0.306263 1:-58821325 MsG0180003237.01.T01:CDS
AATTTAAGTTCAGAGCTTAT+TGG 0.325685 1:-58821459 MsG0180003237.01.T01:CDS
AGCCCAAGGAACCTATTCTT+TGG 0.331068 1:-58821433 MsG0180003237.01.T01:CDS
AATTTGGTTCTTTAGTTAAA+GGG 0.362691 1:+58821478 None:intergenic
ATTTGGTATAATTCTACATC+TGG 0.367460 1:-58821225 MsG0180003237.01.T01:CDS
TATTTCCATGACATCCTAGA+TGG 0.372994 1:-58821585 MsG0180003237.01.T01:CDS
TTCTGAGGGTAAACTTATAT+GGG 0.380771 1:+58821638 None:intergenic
ATTTGCAAGGGGTTATGCTT+TGG 0.411059 1:-58821256 MsG0180003237.01.T01:CDS
ATGCCTATTGTTGGAGGAAC+TGG 0.412156 1:-58821288 MsG0180003237.01.T01:CDS
AATTACTTCATGAGTGTTCA+AGG 0.435174 1:-58821684 None:intergenic
GGGAGCAGCCTCACCATGCT+TGG 0.438720 1:-58821342 MsG0180003237.01.T01:CDS
GCACCAGTTCCTCCAACAAT+AGG 0.444460 1:+58821285 None:intergenic
AAGCCAAAGAATAGGTTCCT+TGG 0.451718 1:+58821430 None:intergenic
AGGACCATGAGATGATTCAT+TGG 0.458423 1:+58821527 None:intergenic
CATGGAAATAAAAGTGTAAG+TGG 0.459702 1:+58821598 None:intergenic
AGCCAAAGAATAGGTTCCTT+GGG 0.475806 1:+58821431 None:intergenic
GTCAGAGAAATGCCTATTGT+TGG 0.479243 1:-58821297 MsG0180003237.01.T01:CDS
TTGTTGAGAAGCCAAAGAAT+AGG 0.495361 1:+58821422 None:intergenic
GGAGCAGCCTCACCATGCTT+GGG 0.516739 1:-58821341 MsG0180003237.01.T01:CDS
TTTACAGCTGGAACCTACAA+AGG 0.527260 1:-58821363 MsG0180003237.01.T01:CDS
TTTCACCATCTAGGATGTCA+TGG 0.529125 1:+58821580 None:intergenic
GGACCATGAGATGATTCATT+GGG 0.532395 1:+58821528 None:intergenic
TTACAGCTGGAACCTACAAA+GGG 0.548749 1:-58821362 MsG0180003237.01.T01:CDS
CTGGTGCTTTCAGATTTGCA+AGG 0.550900 1:-58821269 MsG0180003237.01.T01:CDS
AGTATGTAAGTGGCTCCAAA+AGG 0.553653 1:+58821507 None:intergenic
TGGTGCTTTCAGATTTGCAA+GGG 0.553765 1:-58821268 MsG0180003237.01.T01:CDS
CCATGAGATGATTCATTGGG+TGG 0.558415 1:+58821531 None:intergenic
AGGGTTGTCTAGTATGTAAG+TGG 0.563851 1:+58821497 None:intergenic
AGAGAAATGCCTATTGTTGG+AGG 0.564407 1:-58821294 MsG0180003237.01.T01:CDS
TATTCTTTGGCTTCTCAACA+AGG 0.573141 1:-58821420 MsG0180003237.01.T01:CDS
ATGGAAATAAAAGTGTAAGT+GGG 0.573723 1:+58821599 None:intergenic
GAGCTTATTGGAAGAGCCCA+AGG 0.578477 1:-58821447 MsG0180003237.01.T01:CDS
GCAGCCTCACCATGCTTGGG+AGG 0.595405 1:-58821338 MsG0180003237.01.T01:CDS
AATAACATTCCTCCCAAGCA+TGG 0.596205 1:+58821329 None:intergenic
CCACCCAATGAATCATCTCA+TGG 0.605763 1:-58821531 MsG0180003237.01.T01:CDS
CATTCCTCCCAAGCATGGTG+AGG 0.641930 1:+58821334 None:intergenic
GGTGCTTTCAGATTTGCAAG+GGG 0.678373 1:-58821267 MsG0180003237.01.T01:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!!! AAATTTGGTTCTTTAGTTAA+AGG + Chr1:58821383-58821402 None:intergenic 20.0%
!!! AATTTGGTTCTTTAGTTAAA+GGG + Chr1:58821382-58821401 None:intergenic 20.0%
!!! ATTAATTTTGTTTTTACAGC+TGG - Chr1:58821482-58821501 MsG0180003237.01.T01:CDS 20.0%
! ATTTGGTATAATTCTACATC+TGG - Chr1:58821632-58821651 MsG0180003237.01.T01:CDS 25.0%
!! AAAACTTTGTAGATTTTCTG+AGG + Chr1:58821237-58821256 None:intergenic 25.0%
!! AAACTTTGTAGATTTTCTGA+GGG + Chr1:58821236-58821255 None:intergenic 25.0%
!! AATTTAAGTTCAGAGCTTAT+TGG - Chr1:58821398-58821417 MsG0180003237.01.T01:CDS 25.0%
!! ATGGAAATAAAAGTGTAAGT+GGG + Chr1:58821261-58821280 None:intergenic 25.0%
!!! ATAAGCTCTGAACTTAAATT+TGG + Chr1:58821398-58821417 None:intergenic 25.0%
!!! TTGATAATTTTGAGTGTAGT+TGG + Chr1:58821305-58821324 None:intergenic 25.0%
! CATGGAAATAAAAGTGTAAG+TGG + Chr1:58821262-58821281 None:intergenic 30.0%
! TTCTGAGGGTAAACTTATAT+GGG + Chr1:58821222-58821241 None:intergenic 30.0%
! TTTCTGAGGGTAAACTTATA+TGG + Chr1:58821223-58821242 None:intergenic 30.0%
ATGCTTTGGCTAAGACTATT+TGG - Chr1:58821615-58821634 MsG0180003237.01.T01:CDS 35.0%
TATTTCCATGACATCCTAGA+TGG - Chr1:58821272-58821291 MsG0180003237.01.T01:CDS 35.0%
TTGTTGAGAAGCCAAAGAAT+AGG + Chr1:58821438-58821457 None:intergenic 35.0%
! TATTCTTTGGCTTCTCAACA+AGG - Chr1:58821437-58821456 MsG0180003237.01.T01:CDS 35.0%
!!! TAGTTGGTTTTTCACCATCT+AGG + Chr1:58821289-58821308 None:intergenic 35.0%
AAGCCAAAGAATAGGTTCCT+TGG + Chr1:58821430-58821449 None:intergenic 40.0%
AATAACATTCCTCCCAAGCA+TGG + Chr1:58821531-58821550 None:intergenic 40.0%
AGAGAAATGCCTATTGTTGG+AGG - Chr1:58821563-58821582 MsG0180003237.01.T01:CDS 40.0%
AGCCAAAGAATAGGTTCCTT+GGG + Chr1:58821429-58821448 None:intergenic 40.0%
AGGACCATGAGATGATTCAT+TGG + Chr1:58821333-58821352 None:intergenic 40.0%
AGGGTTGTCTAGTATGTAAG+TGG + Chr1:58821363-58821382 None:intergenic 40.0%
AGTATGTAAGTGGCTCCAAA+AGG + Chr1:58821353-58821372 None:intergenic 40.0%
ATTTGCAAGGGGTTATGCTT+TGG - Chr1:58821601-58821620 MsG0180003237.01.T01:CDS 40.0%
GCTTGGGAGGAATGTTATTA+TGG - Chr1:58821532-58821551 MsG0180003237.01.T01:CDS 40.0%
GGACCATGAGATGATTCATT+GGG + Chr1:58821332-58821351 None:intergenic 40.0%
GTCAGAGAAATGCCTATTGT+TGG - Chr1:58821560-58821579 MsG0180003237.01.T01:CDS 40.0%
TGGTGCTTTCAGATTTGCAA+GGG - Chr1:58821589-58821608 MsG0180003237.01.T01:CDS 40.0%
TTACAGCTGGAACCTACAAA+GGG - Chr1:58821495-58821514 MsG0180003237.01.T01:CDS 40.0%
TTTACAGCTGGAACCTACAA+AGG - Chr1:58821494-58821513 MsG0180003237.01.T01:CDS 40.0%
! GAATCATCTCATGGTCCTTT+TGG - Chr1:58821335-58821354 MsG0180003237.01.T01:CDS 40.0%
! TTTCACCATCTAGGATGTCA+TGG + Chr1:58821280-58821299 None:intergenic 40.0%
AGCCCAAGGAACCTATTCTT+TGG - Chr1:58821424-58821443 MsG0180003237.01.T01:CDS 45.0%
ATGCCTATTGTTGGAGGAAC+TGG - Chr1:58821569-58821588 MsG0180003237.01.T01:CDS 45.0%
CCACCCAATGAATCATCTCA+TGG - Chr1:58821326-58821345 MsG0180003237.01.T01:CDS 45.0%
CCATGAGATGATTCATTGGG+TGG + Chr1:58821329-58821348 None:intergenic 45.0%
CTGGTGCTTTCAGATTTGCA+AGG - Chr1:58821588-58821607 MsG0180003237.01.T01:CDS 45.0%
GGTGCTTTCAGATTTGCAAG+GGG - Chr1:58821590-58821609 MsG0180003237.01.T01:CDS 45.0%
GAGCTTATTGGAAGAGCCCA+AGG - Chr1:58821410-58821429 MsG0180003237.01.T01:CDS 50.0%
GCACCAGTTCCTCCAACAAT+AGG + Chr1:58821575-58821594 None:intergenic 50.0%
CATTCCTCCCAAGCATGGTG+AGG + Chr1:58821526-58821545 None:intergenic 55.0%
GGAGCAGCCTCACCATGCTT+GGG - Chr1:58821516-58821535 MsG0180003237.01.T01:CDS 60.0%
GGTGAGGCTGCTCCCTTTGT+AGG + Chr1:58821510-58821529 None:intergenic 60.0%
GCAGCCTCACCATGCTTGGG+AGG - Chr1:58821519-58821538 MsG0180003237.01.T01:CDS 65.0%
GGGAGCAGCCTCACCATGCT+TGG - Chr1:58821515-58821534 MsG0180003237.01.T01:CDS 65.0%
Chromosome Type Strat End Strand Name
Chr1 gene 58821182 58821697 58821182 ID=MsG0180003237.01;Name=MsG0180003237.01
Chr1 mRNA 58821182 58821697 58821182 ID=MsG0180003237.01.T01;Parent=MsG0180003237.01;Name=MsG0180003237.01.T01;_AED=0.40;_eAED=0.40;_QI=0|-1|0|1|-1|1|1|0|171
Chr1 exon 58821182 58821697 58821182 ID=MsG0180003237.01.T01:exon:3782;Parent=MsG0180003237.01.T01
Chr1 CDS 58821182 58821697 58821182 ID=MsG0180003237.01.T01:cds;Parent=MsG0180003237.01.T01
Gene Sequence

>MsG0180003237.01.T01

ATGAGTGTTCAAGGAAGAGTAGTGTCTTTAGAAGAATCCCATATAAGTTTACCCTCAGAAAATCTACAAAGTTTTACCCACTTACACTTTTATTTCCATGACATCCTAGATGGTGAAAAACCAACTACACTCAAAATTATCAATCCACCCAATGAATCATCTCATGGTCCTTTTGGAGCCACTTACATACTAGACAACCCTTTAACTAAAGAACCAAATTTAAGTTCAGAGCTTATTGGAAGAGCCCAAGGAACCTATTCTTTGGCTTCTCAACAAGGTGATTTTGCATTTAAGATCGATATTAATTTTGTTTTTACAGCTGGAACCTACAAAGGGAGCAGCCTCACCATGCTTGGGAGGAATGTTATTATGGATGAAGTCAGAGAAATGCCTATTGTTGGAGGAACTGGTGCTTTCAGATTTGCAAGGGGTTATGCTTTGGCTAAGACTATTTGGTATAATTCTACATCTGGAAATGCTATTGAAGAGTTTGATATTATAATTTTACATTTCTAA

Protein sequence

>MsG0180003237.01.T01

MSVQGRVVSLEESHISLPSENLQSFTHLHFYFHDILDGEKPTTLKIINPPNESSHGPFGATYILDNPLTKEPNLSSELIGRAQGTYSLASQQGDFAFKIDINFVFTAGTYKGSSLTMLGRNVIMDEVREMPIVGGTGAFRFARGYALAKTIWYNSTSGNAIEEFDIIILHF*