AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar ZhongmuNo.1 / MsG0880042915.01

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsG0880042915.01.T01 MTR_8g024690 87.732 269 14 5 1 266 1 253 4.08e-164 455
MsG0880042915.01.T01 MTR_8g024690 87.500 264 14 5 1 261 1 248 1.01e-157 442
MsG0880042915.01.T01 MTR_3g100180 68.321 262 64 6 1 261 1 244 1.55e-109 331
MsG0880042915.01.T01 MTR_3g100180 68.321 262 64 6 1 261 1 244 4.82e-108 334
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsG0880042915.01.T01 AT1G09710 42.366 262 119 8 4 261 9 242 8.04e-55 186
MsG0880042915.01.T01 AT1G09710 42.366 262 119 8 4 261 9 242 8.04e-55 186
MsG0880042915.01.T01 AT1G09710 42.366 262 119 8 4 261 9 242 1.65e-54 186
MsG0880042915.01.T01 AT1G58220 42.692 260 119 9 4 261 6 237 3.34e-50 176
MsG0880042915.01.T01 AT1G58220 42.692 260 119 9 4 261 6 237 4.36e-50 176
MsG0880042915.01.T01 AT1G09710 42.917 240 105 8 26 261 2 213 5.00e-49 170
MsG0880042915.01.T01 AT1G09710 42.917 240 105 8 26 261 2 213 5.00e-49 170

Find 70 sgRNAs with CRISPR-Local

Find 206 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
CGTCCCCTTGTTTGAAATCT+TGG 0.147671 8:+15845741 None:intergenic
TTCCAATCAAATTTGGTATC+AGG 0.181230 8:+15845854 None:intergenic
CAATTCATTCCAATCAAATT+TGG 0.191519 8:+15845847 None:intergenic
GACCTTATTGACGTTGTTTC+AGG 0.199531 8:-15845891 MsG0880042915.01.T01:CDS
CCTCAATTGTTGAGCTATTA+GGG 0.224586 8:+15843094 None:intergenic
GCCTCAATTGTTGAGCTATT+AGG 0.241673 8:+15843093 None:intergenic
TCTGTTGCACTTCTCTTAAT+AGG 0.263867 8:+15842769 None:intergenic
ACTGGAAAATTTACAGTCAT+TGG 0.296643 8:+15843069 None:intergenic
TTCTGTCTCTGAACAGTAAC+TGG 0.300327 8:+15842973 None:intergenic
GTCCCCTTGTTTGAAATCTT+GGG 0.302398 8:+15845742 None:intergenic
TTCGGAGAAGGTAACCTTCT+TGG 0.304445 8:+15846052 None:intergenic
AGAGATGCGGTGAAGGGAAT+TGG 0.330563 8:-15842819 MsG0880042915.01.T01:CDS
CTCGGCGTTGTTTAATCATC+AGG 0.350292 8:-15843151 MsG0880042915.01.T01:CDS
GTTCTAGATGAACGGTAAAC+TGG 0.360722 8:+15843051 None:intergenic
CAAATTTGATTGGAATGAAT+TGG 0.371586 8:-15845846 MsG0880042915.01.T01:CDS
TCGGAGAAGGTAACCTTCTT+GGG 0.378162 8:+15846053 None:intergenic
ATGATTCCAGCTCGCAATCT+AGG 0.387454 8:+15843689 None:intergenic
TTCACAGCCCTCTAATTTGA+CGG 0.404084 8:-15843016 MsG0880042915.01.T01:CDS
TTCCTGAAACAACGTCAATA+AGG 0.413081 8:+15845889 None:intergenic
ACCCTGATACCAAATTTGAT+TGG 0.413869 8:-15845856 MsG0880042915.01.T01:CDS
TGTCTGTTCCGTCAAATTAG+AGG 0.415678 8:+15843008 None:intergenic
AAAAGAATGGTCAGAGGAAG+AGG 0.418034 8:-15842866 MsG0880042915.01.T01:CDS
TTAGAAACCAAGAGAATAGT+TGG 0.438935 8:-15842922 MsG0880042915.01.T01:CDS
GGTACCACCAACTATTCTCT+TGG 0.446261 8:+15842915 None:intergenic
CTATGGCGCCGCTTGGCGTA+TGG 0.469893 8:-15845776 MsG0880042915.01.T01:CDS
GAGATGCGGTGAAGGGAATT+GGG 0.473351 8:-15842818 MsG0880042915.01.T01:CDS
TCCAATCAAATTTGGTATCA+GGG 0.474839 8:+15845855 None:intergenic
CCGTTCATCTAGAACTTCAA+AGG 0.500114 8:-15843043 MsG0880042915.01.T01:CDS
CCATAGCATCTGATACTCTC+TGG 0.501959 8:+15845793 None:intergenic
TCAGATGCTATGGCGCCGCT+TGG 0.503407 8:-15845783 MsG0880042915.01.T01:CDS
TCCCCTTGTTTGAAATCTTG+GGG 0.504008 8:+15845743 None:intergenic
CCCTAATAGCTCAACAATTG+AGG 0.513454 8:-15843094 MsG0880042915.01.T01:CDS
CCAGTACCACAGCTTCCAAA+AGG 0.521688 8:-15842894 MsG0880042915.01.T01:CDS
GCTGTTCAGAGATGCGGTGA+AGG 0.527721 8:-15842826 MsG0880042915.01.T01:CDS
ATTAAACAACGCCGAGAACC+AGG 0.528802 8:+15843158 None:intergenic
CCAGAGAGTATCAGATGCTA+TGG 0.533322 8:-15845793 MsG0880042915.01.T01:CDS
CCTTTGAAGTTCTAGATGAA+CGG 0.536271 8:+15843043 None:intergenic
GGTCCGTCTCATACTCCCTC+CGG 0.536470 8:-15845708 MsG0880042915.01.T01:intron
GTCTGTTCCGTCAAATTAGA+GGG 0.537189 8:+15843009 None:intergenic
AATTGGGTAGCCATGGCAAA+AGG 0.538707 8:-15842802 MsG0880042915.01.T01:CDS
TTGTTTGAAATCTTGGGGCG+AGG 0.543178 8:+15845748 None:intergenic
AAGTGCAACAGAATTGTCTC+AGG 0.543345 8:-15842758 MsG0880042915.01.T01:CDS
TTGGTGAACAAAACTTCCAC+TGG 0.548762 8:-15845827 MsG0880042915.01.T01:CDS
CCAAAAGGAAAAGAAAAGAA+TGG 0.553085 8:-15842879 MsG0880042915.01.T01:CDS
ACAAGGGGACGATGAACCTA+TGG 0.553695 8:-15845729 MsG0880042915.01.T01:CDS
TCGCCCCAAGATTTCAAACA+AGG 0.557645 8:-15845746 MsG0880042915.01.T01:CDS
GAAAAGAAAAGAATGGTCAG+AGG 0.561735 8:-15842872 MsG0880042915.01.T01:CDS
TGATGTAGCTTCAACATGTA+CGG 0.566855 8:+15843660 None:intergenic
GGAAGATTCACTTAGCGTGC+GGG 0.567154 8:+15843116 None:intergenic
GGAAGAGGACAATCAGCTAC+GGG 0.574165 8:-15842851 MsG0880042915.01.T01:CDS
CTGTTCAGAGATGCGGTGAA+GGG 0.582365 8:-15842825 MsG0880042915.01.T01:CDS
GCCAGAAAATAATCCCAAGA+AGG 0.583604 8:-15846066 MsG0880042915.01.T01:CDS
CGGGCTGCTGTTCAGAGATG+CGG 0.586482 8:-15842832 MsG0880042915.01.T01:CDS
AGGAAGAGGACAATCAGCTA+CGG 0.586801 8:-15842852 MsG0880042915.01.T01:CDS
TGAAGGGAATTGGGTAGCCA+TGG 0.588079 8:-15842809 MsG0880042915.01.T01:CDS
GGGAGTATGAGACGGACCAT+AGG 0.590555 8:+15845713 None:intergenic
TGCAACAGAATTGTCTCAGG+TGG 0.598702 8:-15842755 MsG0880042915.01.T01:CDS
CGCCCCAAGATTTCAAACAA+GGG 0.599788 8:-15845745 MsG0880042915.01.T01:CDS
GGGAAGATTCACTTAGCGTG+CGG 0.612099 8:+15843115 None:intergenic
CTCAATTGTTGAGCTATTAG+GGG 0.614673 8:+15843095 None:intergenic
GTAGCTTCAACATGTACGGA+AGG 0.615313 8:+15843664 None:intergenic
AGGAACAACCATACGCCAAG+CGG 0.617202 8:+15845768 None:intergenic
ATTCACCTTCGCACATGCTG+AGG 0.618037 8:+15843633 None:intergenic
GCCCCAAGATTTCAAACAAG+GGG 0.628345 8:-15845744 MsG0880042915.01.T01:CDS
TAGTGACCTAGATTGCGAGC+TGG 0.630707 8:-15843695 MsG0880042915.01.T01:CDS
AGACCGGAGGGAGTATGAGA+CGG 0.641157 8:+15845705 None:intergenic
ATACCGTTGTGTGATTGAAG+CGG 0.642344 8:+15846022 None:intergenic
GAAACCAAGAGAATAGTTGG+TGG 0.642501 8:-15842919 MsG0880042915.01.T01:CDS
TCTGGCATTAGAAATACCAG+TGG 0.709866 8:+15845811 None:intergenic
ACGCCGCTTCAATCACACAA+CGG 0.764373 8:-15846025 MsG0880042915.01.T01:intron

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! AAATATTATATTATTAATTA+AGG - Chr8:15843664-15843683 MsG0880042915.01.T01:CDS 0.0%
!! ATAAATATAGATAACAAAAT+AGG + Chr8:15843456-15843475 None:intergenic 10.0%
!! TAAATATAGATAACAAAATA+GGG + Chr8:15843455-15843474 None:intergenic 10.0%
!! AAAGATAAAATAACGTTAAA+TGG - Chr8:15844269-15844288 MsG0880042915.01.T01:intron 15.0%
!! AAATATAGATAACAAAATAG+GGG + Chr8:15843454-15843473 None:intergenic 15.0%
!! ATTTCTGATAATTGATTATT+TGG - Chr8:15844149-15844168 MsG0880042915.01.T01:intron 15.0%
!! TTTCTGATAATTGATTATTT+GGG - Chr8:15844150-15844169 MsG0880042915.01.T01:intron 15.0%
!!! AATGTTTTTACTGAAAAATA+TGG - Chr8:15845501-15845520 MsG0880042915.01.T01:intron 15.0%
!!! TGCAAATAAATAAACTTTTA+TGG - Chr8:15843278-15843297 MsG0880042915.01.T01:intron 15.0%
!!! TTTTTTGTTTTCATATTTTC+AGG - Chr8:15845084-15845103 MsG0880042915.01.T01:intron 15.0%
!! AAAAAATAAGTCAACTCAAA+TGG + Chr8:15843251-15843270 None:intergenic 20.0%
!! AATACTGTTTCATTTAATAG+AGG + Chr8:15845294-15845313 None:intergenic 20.0%
!! ATCTATCAAACAACAAAAAT+AGG + Chr8:15845539-15845558 None:intergenic 20.0%
!! TTATTTATAAAGATCCTCAA+TGG - Chr8:15845339-15845358 MsG0880042915.01.T01:intron 20.0%
!! TTCTGATAATTGATTATTTG+GGG - Chr8:15844151-15844170 MsG0880042915.01.T01:intron 20.0%
!! TTGTAATTCTAGTAAAAATG+CGG - Chr8:15845415-15845434 MsG0880042915.01.T01:intron 20.0%
!!! AATTTAATTTCGGTTTATTC+TGG - Chr8:15844850-15844869 MsG0880042915.01.T01:intron 20.0%
!!! TCATTTTGACTGTAAAAAAA+AGG - Chr8:15843872-15843891 MsG0880042915.01.T01:intron 20.0%
! AAAAAACAAGTCAATCCAAA+CGG - Chr8:15843426-15843445 MsG0880042915.01.T01:intron 25.0%
! AATAAGCTATTTATCCAAAC+AGG + Chr8:15844177-15844196 None:intergenic 25.0%
! AATAATCATAGTTGTCAAAG+CGG - Chr8:15843593-15843612 MsG0880042915.01.T01:intron 25.0%
! ATTAAATGAAACAGTATTCG+TGG - Chr8:15845296-15845315 MsG0880042915.01.T01:intron 25.0%
! CAAATTTGATTGGAATGAAT+TGG - Chr8:15842963-15842982 MsG0880042915.01.T01:CDS 25.0%
! CAATTCATTCCAATCAAATT+TGG + Chr8:15842965-15842984 None:intergenic 25.0%
! TATCACAATGCATTAATTCA+AGG - Chr8:15844813-15844832 MsG0880042915.01.T01:intron 25.0%
! TCTAAAAAATACTATGAGAC+CGG + Chr8:15843123-15843142 None:intergenic 25.0%
! TTTGAATTACTGCAATGTAT+TGG - Chr8:15844557-15844576 MsG0880042915.01.T01:intron 25.0%
!! AACTGATTAGTCATAGAATA+TGG + Chr8:15844785-15844804 None:intergenic 25.0%
!! AAGATACAATTTTCGTTAGT+GGG - Chr8:15843347-15843366 MsG0880042915.01.T01:intron 25.0%
!! ATACTGGCACAATTTAATTT+CGG - Chr8:15844840-15844859 MsG0880042915.01.T01:intron 25.0%
!!! ATTTTTGTTTCGTAGATTCT+TGG - Chr8:15845029-15845048 MsG0880042915.01.T01:intron 25.0%
!!! TAAGTTTTCTGAGACTATTT+TGG + Chr8:15844388-15844407 None:intergenic 25.0%
AAAAACAAGTCAATCCAAAC+GGG - Chr8:15843427-15843446 MsG0880042915.01.T01:intron 30.0%
ACAGTCAAAATGAAGAAGAA+AGG + Chr8:15843865-15843884 None:intergenic 30.0%
ACTGGAAAATTTACAGTCAT+TGG + Chr8:15845743-15845762 None:intergenic 30.0%
ATGTTTGTGTCTGTTATTGT+TGG - Chr8:15843549-15843568 MsG0880042915.01.T01:intron 30.0%
ATTAATATCTGACAGTGATG+CGG - Chr8:15845448-15845467 MsG0880042915.01.T01:intron 30.0%
CAGTCAAAATGAAGAAGAAA+GGG + Chr8:15843864-15843883 None:intergenic 30.0%
CATTGAAACCATAAAGAACT+TGG + Chr8:15845584-15845603 None:intergenic 30.0%
CCAAAAGGAAAAGAAAAGAA+TGG - Chr8:15845930-15845949 MsG0880042915.01.T01:intron 30.0%
CCTTGAATAATTGATGTATC+CGG - Chr8:15843162-15843181 MsG0880042915.01.T01:intron 30.0%
GCAAATTTCTTTCTGATTGT+GGG - Chr8:15845381-15845400 MsG0880042915.01.T01:intron 30.0%
GTAGATAAGCTAATCCAAAA+AGG - Chr8:15844413-15844432 MsG0880042915.01.T01:intron 30.0%
TATCTGCTTCATAAACAAAC+CGG + Chr8:15843184-15843203 None:intergenic 30.0%
TCCAATCAAATTTGGTATCA+GGG + Chr8:15842957-15842976 None:intergenic 30.0%
TGCAAATTTCTTTCTGATTG+TGG - Chr8:15845380-15845399 MsG0880042915.01.T01:intron 30.0%
TGGAATAACCAAGTTCTTTA+TGG - Chr8:15845573-15845592 MsG0880042915.01.T01:intron 30.0%
TGTAGTAAGTAGTTCATACA+AGG - Chr8:15844702-15844721 MsG0880042915.01.T01:intron 30.0%
TTAAATGTGTGATTCCATTG+AGG + Chr8:15845356-15845375 None:intergenic 30.0%
TTAGAAACCAAGAGAATAGT+TGG - Chr8:15845887-15845906 MsG0880042915.01.T01:CDS 30.0%
TTCCAATCAAATTTGGTATC+AGG + Chr8:15842958-15842977 None:intergenic 30.0%
TTGGTTATTCCACACTAAAT+GGG + Chr8:15845565-15845584 None:intergenic 30.0%
! ATTAATTCAAGGCATCATAC+TGG - Chr8:15844824-15844843 MsG0880042915.01.T01:intron 30.0%
! CCATTCTTTTCTTTTCCTTT+TGG + Chr8:15845933-15845952 None:intergenic 30.0%
! GAAGATACAATTTTCGTTAG+TGG - Chr8:15843346-15843365 MsG0880042915.01.T01:intron 30.0%
!! ATTGGAAAGCACCTTAATTT+CGG - Chr8:15844575-15844594 MsG0880042915.01.T01:intron 30.0%
!!! AATCGTTTTTTGTGTTTTGC+AGG - Chr8:15842884-15842903 MsG0880042915.01.T01:CDS 30.0%
!!! TTTTGGATTAGCTTATCTAC+TGG + Chr8:15844413-15844432 None:intergenic 30.0%
AAAAAATACTATGAGACCGG+AGG + Chr8:15843120-15843139 None:intergenic 35.0%
AAAAATACTATGAGACCGGA+GGG + Chr8:15843119-15843138 None:intergenic 35.0%
AAAGCTATTCGAACAAAACG+AGG + Chr8:15843527-15843546 None:intergenic 35.0%
ACAAAGGCAGTACATGTAAA+AGG + Chr8:15844928-15844947 None:intergenic 35.0%
ACCCTGATACCAAATTTGAT+TGG - Chr8:15842953-15842972 MsG0880042915.01.T01:CDS 35.0%
AGTCCTATAGCTGTATGATA+AGG + Chr8:15845478-15845497 None:intergenic 35.0%
ATAGATAGTCCCATTTAGTG+TGG - Chr8:15845553-15845572 MsG0880042915.01.T01:intron 35.0%
CAAAGGCAGTACATGTAAAA+GGG + Chr8:15844927-15844946 None:intergenic 35.0%
CCGGATACATCAATTATTCA+AGG + Chr8:15843165-15843184 None:intergenic 35.0%
CCTCAATTGTTGAGCTATTA+GGG + Chr8:15845718-15845737 None:intergenic 35.0%
CTAGGTCTCAAATATAAGCA+CGG + Chr8:15843225-15843244 None:intergenic 35.0%
CTCAATTGTTGAGCTATTAG+GGG + Chr8:15845717-15845736 None:intergenic 35.0%
CTTGGTTATTCCACACTAAA+TGG + Chr8:15845566-15845585 None:intergenic 35.0%
GAAAAGAAAAGAATGGTCAG+AGG - Chr8:15845937-15845956 MsG0880042915.01.T01:intron 35.0%
GATTAGAGATCTAGAGATCT+AGG + Chr8:15843826-15843845 None:intergenic 35.0%
GCTAAAAGTTTCCAGAACTA+AGG + Chr8:15844614-15844633 None:intergenic 35.0%
TCTGTTGCACTTCTCTTAAT+AGG + Chr8:15846043-15846062 None:intergenic 35.0%
TGATGTAGCTTCAACATGTA+CGG + Chr8:15845152-15845171 None:intergenic 35.0%
TGTTTCGTAGATTCTTGGAT+AGG - Chr8:15845034-15845053 MsG0880042915.01.T01:intron 35.0%
TTCCTGAAACAACGTCAATA+AGG + Chr8:15842923-15842942 None:intergenic 35.0%
TTGCCTTATCATACAGCTAT+AGG - Chr8:15845472-15845491 MsG0880042915.01.T01:intron 35.0%
TTTGCAGCTTATAACACAAG+TGG - Chr8:15844195-15844214 MsG0880042915.01.T01:intron 35.0%
! ACCTTCTTGGGATTATTTTC+TGG + Chr8:15842747-15842766 None:intergenic 35.0%
! AGAACAGAGATGAGATTTTG+TGG - Chr8:15843906-15843925 MsG0880042915.01.T01:intron 35.0%
! GTGCTTATATTTGAGACCTA+GGG - Chr8:15843224-15843243 MsG0880042915.01.T01:intron 35.0%
!! CCTTTGAAGTTCTAGATGAA+CGG + Chr8:15845769-15845788 None:intergenic 35.0%
AAAAGAATGGTCAGAGGAAG+AGG - Chr8:15845943-15845962 MsG0880042915.01.T01:intron 40.0%
AAGTGCAACAGAATTGTCTC+AGG - Chr8:15846051-15846070 MsG0880042915.01.T01:CDS 40.0%
ACCTAACTGGAAATGGCAAT+TGG + Chr8:15844958-15844977 None:intergenic 40.0%
ATACCAACAGTAGTTAGCTC+AGG + Chr8:15844505-15844524 None:intergenic 40.0%
ATACCGTTGTGTGATTGAAG+CGG + Chr8:15842790-15842809 None:intergenic 40.0%
ATACCTGAGCTAACTACTGT+TGG - Chr8:15844499-15844518 MsG0880042915.01.T01:intron 40.0%
ATTGCCTCTGTTGTCAATAG+CGG + Chr8:15844118-15844137 None:intergenic 40.0%
CCCTAATAGCTCAACAATTG+AGG - Chr8:15845715-15845734 MsG0880042915.01.T01:CDS 40.0%
CTAATGCATCACCACATAAC+TGG + Chr8:15844752-15844771 None:intergenic 40.0%
GAAACCAAGAGAATAGTTGG+TGG - Chr8:15845890-15845909 MsG0880042915.01.T01:CDS 40.0%
GAAGCCGAATGCTCAAATAA+AGG + Chr8:15844639-15844658 None:intergenic 40.0%
GCCAGAAAATAATCCCAAGA+AGG - Chr8:15842743-15842762 MsG0880042915.01.T01:CDS 40.0%
GCCTCAATTGTTGAGCTATT+AGG + Chr8:15845719-15845738 None:intergenic 40.0%
GGCAATTGGAAGAGTAACAA+AGG + Chr8:15844944-15844963 None:intergenic 40.0%
GTCTGTTCCGTCAAATTAGA+GGG + Chr8:15845803-15845822 None:intergenic 40.0%
TCTGGCATTAGAAATACCAG+TGG + Chr8:15843001-15843020 None:intergenic 40.0%
TGAAGACGATCTAGAGATCT+AGG + Chr8:15843786-15843805 None:intergenic 40.0%
TGTCTGTTCCGTCAAATTAG+AGG + Chr8:15845804-15845823 None:intergenic 40.0%
TGTGCAAACCTAACTGGAAA+TGG + Chr8:15844965-15844984 None:intergenic 40.0%
TTCACAGCCCTCTAATTTGA+CGG - Chr8:15845793-15845812 MsG0880042915.01.T01:CDS 40.0%
TTCTGTCTCTGAACAGTAAC+TGG + Chr8:15845839-15845858 None:intergenic 40.0%
TTGGTGAACAAAACTTCCAC+TGG - Chr8:15842982-15843001 MsG0880042915.01.T01:CDS 40.0%
! AAATCTGTCTCCTTTTGCCA+TGG + Chr8:15846020-15846039 None:intergenic 40.0%
! CCGTTCATCTAGAACTTCAA+AGG - Chr8:15845766-15845785 MsG0880042915.01.T01:CDS 40.0%
! CGTGCTTATATTTGAGACCT+AGG - Chr8:15843223-15843242 MsG0880042915.01.T01:intron 40.0%
! CTTGTAACAAGAGGCCTTTT+TGG + Chr8:15844430-15844449 None:intergenic 40.0%
! GACCTTATTGACGTTGTTTC+AGG - Chr8:15842918-15842937 MsG0880042915.01.T01:CDS 40.0%
! GCTGCCTTTATTTGAGCATT+CGG - Chr8:15844632-15844651 MsG0880042915.01.T01:intron 40.0%
! GTCCCCTTGTTTGAAATCTT+GGG + Chr8:15843070-15843089 None:intergenic 40.0%
! GTTCTAGATGAACGGTAAAC+TGG + Chr8:15845761-15845780 None:intergenic 40.0%
! TCCAATTGCCATTTCCAGTT+AGG - Chr8:15844954-15844973 MsG0880042915.01.T01:intron 40.0%
! TCCCCTTGTTTGAAATCTTG+GGG + Chr8:15843069-15843088 None:intergenic 40.0%
! TTGATTATTTGGGGCCTGTT+TGG - Chr8:15844160-15844179 MsG0880042915.01.T01:intron 40.0%
! TTTCCAGAACTAAGGCACTA+GGG + Chr8:15844606-15844625 None:intergenic 40.0%
!! TCTTTTCCTTTTGGAAGCTG+TGG + Chr8:15845924-15845943 None:intergenic 40.0%
!! TTCTTCAATTTTGACCAGCC+TGG - Chr8:15845633-15845652 MsG0880042915.01.T01:intron 40.0%
AACAAAATAGGGGACCCGTT+TGG + Chr8:15843444-15843463 None:intergenic 45.0%
AATTGGGTAGCCATGGCAAA+AGG - Chr8:15846007-15846026 MsG0880042915.01.T01:intron 45.0%
ACAAGTGACGGTGCTTATGT+GGG - Chr8:15844445-15844464 MsG0880042915.01.T01:intron 45.0%
AGCACGGATTCTTTCTTCAG+CGG + Chr8:15843209-15843228 None:intergenic 45.0%
AGGAAGAGGACAATCAGCTA+CGG - Chr8:15845957-15845976 MsG0880042915.01.T01:intron 45.0%
AGGCAGTACATGTAAAAGGG+TGG + Chr8:15844924-15844943 None:intergenic 45.0%
AGGCCTCTTGTTACAAGTGA+CGG - Chr8:15844433-15844452 MsG0880042915.01.T01:intron 45.0%
AGTCAACTCAAATGGACCCT+AGG + Chr8:15843243-15843262 None:intergenic 45.0%
ATGATTCCAGCTCGCAATCT+AGG + Chr8:15845123-15845142 None:intergenic 45.0%
ATTAAACAACGCCGAGAACC+AGG + Chr8:15845654-15845673 None:intergenic 45.0%
CACCACATAACTGGTGCTTA+AGG + Chr8:15844743-15844762 None:intergenic 45.0%
CCATAGCATCTGATACTCTC+TGG + Chr8:15843019-15843038 None:intergenic 45.0%
CGCCCCAAGATTTCAAACAA+GGG - Chr8:15843064-15843083 MsG0880042915.01.T01:CDS 45.0%
CTAGGGGAAGACCGAAATTA+AGG + Chr8:15844589-15844608 None:intergenic 45.0%
CTCGGCGTTGTTTAATCATC+AGG - Chr8:15845658-15845677 MsG0880042915.01.T01:intron 45.0%
GCCCCAAGATTTCAAACAAG+GGG - Chr8:15843065-15843084 MsG0880042915.01.T01:CDS 45.0%
GCTGATTGTGCAAACCTAAC+TGG + Chr8:15844971-15844990 None:intergenic 45.0%
GGTACCACCAACTATTCTCT+TGG + Chr8:15845897-15845916 None:intergenic 45.0%
GTAGCTTCAACATGTACGGA+AGG + Chr8:15845148-15845167 None:intergenic 45.0%
TACAAGTGACGGTGCTTATG+TGG - Chr8:15844444-15844463 MsG0880042915.01.T01:intron 45.0%
TAGACCGCTATTGACAACAG+AGG - Chr8:15844111-15844130 MsG0880042915.01.T01:intron 45.0%
TAGTTGTCAAAGCGGAAACG+CGG - Chr8:15843601-15843620 MsG0880042915.01.T01:intron 45.0%
TCGCCCCAAGATTTCAAACA+AGG - Chr8:15843063-15843082 MsG0880042915.01.T01:CDS 45.0%
TCGGAGAAGGTAACCTTCTT+GGG + Chr8:15842759-15842778 None:intergenic 45.0%
TGCAACAGAATTGTCTCAGG+TGG - Chr8:15846054-15846073 MsG0880042915.01.T01:CDS 45.0%
TTCGGAGAAGGTAACCTTCT+TGG + Chr8:15842760-15842779 None:intergenic 45.0%
TTGTCAATAGCGGTCTAACG+CGG + Chr8:15844108-15844127 None:intergenic 45.0%
! CGTCCCCTTGTTTGAAATCT+TGG + Chr8:15843071-15843090 None:intergenic 45.0%
! GTTTCCAGAACTAAGGCACT+AGG + Chr8:15844607-15844626 None:intergenic 45.0%
! TTCCAGAACTAAGGCACTAG+GGG + Chr8:15844605-15844624 None:intergenic 45.0%
! TTGTTTGAAATCTTGGGGCG+AGG + Chr8:15843064-15843083 None:intergenic 45.0%
!! ATTTTGACCAGCCTGGTTCT+CGG - Chr8:15845640-15845659 MsG0880042915.01.T01:intron 45.0%
!! CCAGAGAGTATCAGATGCTA+TGG - Chr8:15843016-15843035 MsG0880042915.01.T01:CDS 45.0%
ACAAGGGGACGATGAACCTA+TGG - Chr8:15843080-15843099 MsG0880042915.01.T01:CDS 50.0%
ACGCCGCTTCAATCACACAA+CGG - Chr8:15842784-15842803 MsG0880042915.01.T01:CDS 50.0%
AGAGATGCGGTGAAGGGAAT+TGG - Chr8:15845990-15846009 MsG0880042915.01.T01:intron 50.0%
AGGAACAACCATACGCCAAG+CGG + Chr8:15843044-15843063 None:intergenic 50.0%
AGGCCAATGCGGAACAATAG+CGG + Chr8:15844020-15844039 None:intergenic 50.0%
ATTCACCTTCGCACATGCTG+AGG + Chr8:15845179-15845198 None:intergenic 50.0%
CCAGTACCACAGCTTCCAAA+AGG - Chr8:15845915-15845934 MsG0880042915.01.T01:intron 50.0%
CGCCTTAAGCACCAGTTATG+TGG - Chr8:15844738-15844757 MsG0880042915.01.T01:intron 50.0%
CTGTTCAGAGATGCGGTGAA+GGG - Chr8:15845984-15846003 MsG0880042915.01.T01:intron 50.0%
GAGATGCGGTGAAGGGAATT+GGG - Chr8:15845991-15846010 MsG0880042915.01.T01:intron 50.0%
GCACCGTCACTTGTAACAAG+AGG + Chr8:15844439-15844458 None:intergenic 50.0%
GGAAGAGGACAATCAGCTAC+GGG - Chr8:15845958-15845977 MsG0880042915.01.T01:intron 50.0%
GGAAGATTCACTTAGCGTGC+GGG + Chr8:15845696-15845715 None:intergenic 50.0%
GGGAAGATTCACTTAGCGTG+CGG + Chr8:15845697-15845716 None:intergenic 50.0%
GGTTCAACGCGGGCAAAAAA+CGG + Chr8:15843957-15843976 None:intergenic 50.0%
GTTCAACGCGGGCAAAAAAC+GGG + Chr8:15843956-15843975 None:intergenic 50.0%
TAGTGACCTAGATTGCGAGC+TGG - Chr8:15845114-15845133 MsG0880042915.01.T01:intron 50.0%
TGAAGGGAATTGGGTAGCCA+TGG - Chr8:15846000-15846019 MsG0880042915.01.T01:intron 50.0%
TTCCCCTAGTGCCTTAGTTC+TGG - Chr8:15844600-15844619 MsG0880042915.01.T01:intron 50.0%
!! CCTTTTGGAAGCTGTGGTAC+TGG + Chr8:15845918-15845937 None:intergenic 50.0%
!! GATTGAAGCGGCGTCGTTTT+CGG + Chr8:15842778-15842797 None:intergenic 50.0%
AGAGTCCTCAGCATGTGCGA+AGG - Chr8:15845171-15845190 MsG0880042915.01.T01:intron 55.0%
ATTCGGCTTCGTCACTGTGC+TGG - Chr8:15844649-15844668 MsG0880042915.01.T01:intron 55.0%
GACCCGCTATTGTTCCGCAT+TGG - Chr8:15844014-15844033 MsG0880042915.01.T01:intron 55.0%
GCTGTTCAGAGATGCGGTGA+AGG - Chr8:15845983-15846002 MsG0880042915.01.T01:intron 55.0%
GGCAAAAAGCGGTTCAACGC+GGG + Chr8:15843967-15843986 None:intergenic 55.0%
GGCCAATGCGGAACAATAGC+GGG + Chr8:15844019-15844038 None:intergenic 55.0%
GGGCAAAAAGCGGTTCAACG+CGG + Chr8:15843968-15843987 None:intergenic 55.0%
TTCGGCTTCGTCACTGTGCT+GGG - Chr8:15844650-15844669 MsG0880042915.01.T01:intron 55.0%
! AGACCGGAGGGAGTATGAGA+CGG + Chr8:15843107-15843126 None:intergenic 55.0%
! GGGAGTATGAGACGGACCAT+AGG + Chr8:15843099-15843118 None:intergenic 55.0%
!! GAACAATAGCGGGTCAACGC+GGG + Chr8:15844009-15844028 None:intergenic 55.0%
!! GGAACAATAGCGGGTCAACG+CGG + Chr8:15844010-15844029 None:intergenic 55.0%
AACAACGCCGAGAACCAGGC+TGG + Chr8:15845650-15845669 None:intergenic 60.0%
AGTCGCGCGTTAGGCCAATG+CGG + Chr8:15844031-15844050 None:intergenic 60.0%
CGGGCTGCTGTTCAGAGATG+CGG - Chr8:15845977-15845996 MsG0880042915.01.T01:intron 60.0%
GCGGGCGCTATTACATGACG+CGG - Chr8:15843630-15843649 MsG0880042915.01.T01:intron 60.0%
GGCAAAAAGCGGGTCAACGC+GGG + Chr8:15843988-15844007 None:intergenic 60.0%
GGGCAAAAAGCGGGTCAACG+CGG + Chr8:15843989-15844008 None:intergenic 60.0%
GGGTCAACGCGGGCAAAAAG+CGG + Chr8:15843978-15843997 None:intergenic 60.0%
GGTCAACGCGGGCAAAAAGC+GGG + Chr8:15843977-15843996 None:intergenic 60.0%
GGTCCGTCTCATACTCCCTC+CGG - Chr8:15843101-15843120 MsG0880042915.01.T01:CDS 60.0%
TCAGATGCTATGGCGCCGCT+TGG - Chr8:15843026-15843045 MsG0880042915.01.T01:CDS 60.0%
! CATTGGCCTAACGCGCGACT+CGG - Chr8:15844031-15844050 MsG0880042915.01.T01:intron 60.0%
!! AGCGGCGTCGTTTTCGGAGA+AGG + Chr8:15842772-15842791 None:intergenic 60.0%
CTATGGCGCCGCTTGGCGTA+TGG - Chr8:15843033-15843052 MsG0880042915.01.T01:CDS 65.0%
GTCAAAGCGGAAACGCGGCG+CGG - Chr8:15843606-15843625 MsG0880042915.01.T01:intron 65.0%
ACGCGACCGAGTCGCGCGTT+AGG + Chr8:15844040-15844059 None:intergenic 70.0%
CGGTCGCGTTCTGACGCGCT+AGG - Chr8:15844051-15844070 MsG0880042915.01.T01:intron 70.0%
GGTCTAACGCGGCGCGATCG+CGG + Chr8:15844097-15844116 None:intergenic 70.0%
! AGCGGAAACGCGGCGCGGCG+CGG - Chr8:15843611-15843630 MsG0880042915.01.T01:intron 80.0%
! GGCGCGATCGCGGCGCGAAG+CGG + Chr8:15844087-15844106 None:intergenic 80.0%
! GCGGAAACGCGGCGCGGCGC+GGG - Chr8:15843612-15843631 MsG0880042915.01.T01:intron 85.0%
Chromosome Type Strat End Strand Name
Chr8 gene 15842741 15846090 15842741 ID=MsG0880042915.01;Name=MsG0880042915.01
Chr8 mRNA 15842741 15846090 15842741 ID=MsG0880042915.01.T01;Parent=MsG0880042915.01;Name=MsG0880042915.01.T01;_AED=0.44;_eAED=0.47;_QI=0|0|0|1|1|1|4|0|266
Chr8 exon 15846026 15846090 15846026 ID=MsG0880042915.01.T01:exon:4078;Parent=MsG0880042915.01.T01
Chr8 exon 15845709 15845925 15845709 ID=MsG0880042915.01.T01:exon:4077;Parent=MsG0880042915.01.T01
Chr8 exon 15843647 15843725 15843647 ID=MsG0880042915.01.T01:exon:4076;Parent=MsG0880042915.01.T01
Chr8 exon 15842741 15843180 15842741 ID=MsG0880042915.01.T01:exon:4075;Parent=MsG0880042915.01.T01
Chr8 CDS 15846026 15846090 15846026 ID=MsG0880042915.01.T01:cds;Parent=MsG0880042915.01.T01
Chr8 CDS 15845709 15845925 15845709 ID=MsG0880042915.01.T01:cds;Parent=MsG0880042915.01.T01
Chr8 CDS 15843647 15843725 15843647 ID=MsG0880042915.01.T01:cds;Parent=MsG0880042915.01.T01
Chr8 CDS 15842741 15843180 15842741 ID=MsG0880042915.01.T01:cds;Parent=MsG0880042915.01.T01
Gene Sequence

>MsG0880042915.01.T01

ATGCCAGAAAATAATCCCAAGAAGGTTACCTTCTCCGAAAACGACGCCGCTTCAATCACACAACGGTATGACACAAAGACCTTATTGACGTTGTTTCAGGAATTATCAAATTACCCTGATACCAAATTTGATTGGAATGAATTGGTGAACAAAACTTCCACTGGTATTTCTAATGCCAGAGAGTATCAGATGCTATGGCGCCGCTTGGCGTATGGTTGTTCCTCGCCCCAAGATTTCAAACAAGGGGACGATGAACCTATGGTCCGTCTCATACTCCCTCCGGATGATGATAGTGACCTAGATTGCGAGCTGGAATCATTTCCTTCCGTACATGTTGAAGCTACATCAGAGTCCTCAGCATCCTGGTTCTCGGCGTTGTTTAATCATCAGGTGATAATTGCTTCCCGCACGCTAAGTGAATCTTCCCCTAATAGCTCAACAATTGAGGCTCCAATGACTGTAAATTTTCCAGTTTACCGTTCATCTAGAACTTCAAAGGAAATTTCACAGCCCTCTAATTTGACGGAACAGACAAGCATTACGTTTCCAGTTACTGTTCAGAGACAGAAACTGCGAACTGTGTCAGCAACAGATGCTTTAGAAACCAAGAGAATAGTTGGTGGTACCAGTACCACAGCTTCCAAAAGGAAAAGAAAAGAATGGTCAGAGGAAGAGGACAATCAGCTACGGGCTGCTGTTCAGAGATGCGGTGAAGGGAATTGGGTAGCCATGGCAAAAGGAGACAGATTTCCTATTAAGAGAAGTGCAACAGAATTGTCTCAGGTGGTTTTTCAACCCTAA
Protein sequence

>MsG0880042915.01.T01

MPENNPKKVTFSENDAASITQRYDTKTLLTLFQELSNYPDTKFDWNELVNKTSTGISNAREYQMLWRRLAYGCSSPQDFKQGDDEPMVRLILPPDDDSDLDCELESFPSVHVEATSESSASWFSALFNHQVIIASRTLSESSPNSSTIEAPMTVNFPVYRSSRTSKEISQPSNLTEQTSITFPVTVQRQKLRTVSATDALETKRIVGGTSTTASKRKRKEWSEEEDNQLRAAVQRCGEGNWVAMAKGDRFPIKRSATELSQVVFQP*