AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar ZhongmuNo.1 / MsG0880043979.01


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsG0880043979.01.T01 MTR_8g045490 75.000 156 21 2 1 139 1 155 1.27e-79 232
MsG0880043979.01.T01 MTR_8g045400 70.513 156 28 2 1 139 1 155 6.93e-74 218
MsG0880043979.01.T01 MTR_8g045520 67.308 156 34 1 1 139 1 156 2.91e-72 213
MsG0880043979.01.T01 MTR_8g045570 63.816 152 38 1 1 135 1 152 2.68e-67 201
MsG0880043979.01.T01 MTR_8g045300 61.538 156 43 1 1 139 1 156 2.11e-65 196
MsG0880043979.01.T01 MTR_8g045695 60.256 156 44 2 1 139 1 155 6.10e-64 192
MsG0880043979.01.T01 MTR_8g045555 62.500 152 40 1 1 135 1 152 4.90e-63 190
MsG0880043979.01.T01 MTR_8g045735 59.355 155 45 2 1 138 1 154 3.66e-62 188
MsG0880043979.01.T01 MTR_8g045665 58.333 156 47 2 1 139 1 155 4.15e-60 182
MsG0880043979.01.T01 MTR_8g045640 58.974 156 46 2 1 139 1 155 6.79e-60 182
MsG0880043979.01.T01 MTR_8g045560 57.692 156 48 2 1 139 1 155 2.69e-58 178
MsG0880043979.01.T01 MTR_1g030810 38.158 152 76 2 1 134 1 152 3.45e-29 104
MsG0880043979.01.T01 MTR_1g030820 36.184 152 79 2 1 134 1 152 4.02e-29 103
MsG0880043979.01.T01 MTR_1g030840 34.839 155 78 5 1 132 1 155 5.75e-20 80.9
MsG0880043979.01.T01 MTR_8g012550 29.167 144 84 3 6 132 8 150 3.90e-17 73.6
MsG0880043979.01.T01 MTR_1g031640 31.973 147 76 5 7 135 7 147 6.63e-16 70.1
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsG0880043979.01.T01 AT1G70870 33.813 139 86 3 1 134 1 138 3.43e-17 73.2
MsG0880043979.01.T01 AT5G28010 28.859 149 88 3 3 133 15 163 8.27e-17 72.8
MsG0880043979.01.T01 AT5G28010 28.859 149 88 3 3 133 15 163 8.27e-17 72.8
MsG0880043979.01.T01 AT1G70890 30.201 149 86 3 3 133 7 155 1.20e-16 72.4
MsG0880043979.01.T01 AT1G23120 36.364 88 55 1 46 133 59 145 2.75e-14 65.9
MsG0880043979.01.T01 AT1G70840 27.517 149 90 3 3 133 20 168 3.29e-14 66.2
MsG0880043979.01.T01 AT1G70830 28.188 149 89 3 3 133 22 170 4.79e-14 65.9
MsG0880043979.01.T01 AT1G23120 36.364 88 55 1 46 133 109 195 4.94e-14 66.2
MsG0880043979.01.T01 AT1G70830 28.188 149 89 3 3 133 22 170 7.46e-14 65.9
MsG0880043979.01.T01 AT1G70830 28.188 149 89 3 3 133 22 170 2.01e-13 65.5
MsG0880043979.01.T01 AT1G70830 28.188 149 89 3 3 133 22 170 2.75e-13 65.9
MsG0880043979.01.T01 AT1G70830 26.846 149 91 3 3 133 184 332 2.02e-11 60.5
MsG0880043979.01.T01 AT1G70880 26.846 149 91 3 3 133 8 156 5.10e-13 62.8
MsG0880043979.01.T01 AT5G28000 26.846 149 91 3 3 133 13 161 8.62e-13 62.4
MsG0880043979.01.T01 AT1G24020 30.921 152 86 4 1 134 1 151 1.22e-12 61.6
MsG0880043979.01.T01 AT1G24020 30.921 152 86 4 1 134 1 151 1.22e-12 61.6
MsG0880043979.01.T01 AT1G70850 26.846 149 91 3 3 133 9 157 2.80e-12 62.0
MsG0880043979.01.T01 AT1G14940 29.286 140 97 2 1 139 1 139 3.10e-12 60.5
MsG0880043979.01.T01 AT1G23130 25.000 148 94 2 3 133 10 157 3.35e-12 60.8
MsG0880043979.01.T01 AT1G70850 26.846 149 91 3 3 133 9 157 4.60e-12 62.4
MsG0880043979.01.T01 AT1G70850 26.846 149 91 3 3 133 9 157 4.60e-12 62.4
MsG0880043979.01.T01 AT1G70830 26.846 149 91 3 3 133 137 285 1.21e-11 60.8
MsG0880043979.01.T01 AT4G23670 28.289 152 91 3 1 135 1 151 4.52e-11 57.4

Find 25 sgRNAs with CRISPR-Local

Find 87 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
TGAGCATTATAGGGTCTTTA+AGG 0.251134 8:-33725716 MsG0880043979.01.T01:CDS
GAGATATTGATGAGCATTAT+AGG 0.261538 8:-33725726 MsG0880043979.01.T01:CDS
AATGGCTACATGGAACATTT+TGG 0.264766 8:-33725601 MsG0880043979.01.T01:CDS
GGTAAGCTCAGTACTGAAAT+TGG 0.267652 8:-33727192 MsG0880043979.01.T01:CDS
ATCTTCACCTTCATGAAGTT+TGG 0.293563 8:+33727083 None:intergenic
ATGGCACTGCTGCTGCTAAA+TGG 0.310932 8:-33725666 MsG0880043979.01.T01:CDS
CACCCTTATCAATTACTTCA+AGG 0.382574 8:+33725689 None:intergenic
AAATGTTCCATGTAGCCATT+TGG 0.413070 8:+33725604 None:intergenic
CATCCTTGAAGTAATTGATA+AGG 0.441221 8:-33725692 MsG0880043979.01.T01:CDS
AACACTTATAAGCTCTTTGG+TGG 0.457999 8:-33725748 MsG0880043979.01.T01:CDS
TTCTAAGAAATGGTGCTCTC+TGG 0.461766 8:-33727213 None:intergenic
TATTTGCGCCTTTGACAAGA+TGG 0.487792 8:+33725554 None:intergenic
ATCCTTGAAGTAATTGATAA+GGG 0.489024 8:-33725691 MsG0880043979.01.T01:CDS
ATTTGCGCCTTTGACAAGAT+GGG 0.520852 8:+33725555 None:intergenic
TGACCCTCCAAATGGCTACA+TGG 0.521176 8:-33725611 MsG0880043979.01.T01:CDS
GAGCATATTGACCCTCCAAA+TGG 0.539495 8:-33725619 MsG0880043979.01.T01:CDS
TGTTCCATGTAGCCATTTGG+AGG 0.542543 8:+33725607 None:intergenic
GAAGTAATTGATAAGGGTGA+TGG 0.549119 8:-33725685 MsG0880043979.01.T01:CDS
AGATATTGATGAGCATTATA+GGG 0.551405 8:-33725725 MsG0880043979.01.T01:CDS
CATCATACCAAACTTCATGA+AGG 0.595182 8:-33727090 MsG0880043979.01.T01:intron
GTTGATGCCCATCTTGTCAA+AGG 0.599351 8:-33725562 MsG0880043979.01.T01:CDS
ATAATTCACAACAGATGGCA+AGG 0.621271 8:-33725821 MsG0880043979.01.T01:intron
GTTCCATGTAGCCATTTGGA+GGG 0.622662 8:+33725608 None:intergenic
TAGATTGTAGAACTTATCAG+AGG 0.623153 8:+33727158 None:intergenic
AGATTGTAGAACTTATCAGA+GGG 0.653447 8:+33727159 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! TATAAAAAATGTTAATTGTT+AGG + Chr8:33725708-33725727 None:intergenic 10.0%
!!! AGTTTATTTGTTAAAAAAAT+AGG - Chr8:33726833-33726852 MsG0880043979.01.T01:intron 10.0%
!!! TGCTTTTAATATTTTTTTTA+TGG + Chr8:33726502-33726521 None:intergenic 10.0%
!! AATCATATAAAGAATATATG+TGG - Chr8:33726703-33726722 MsG0880043979.01.T01:intron 15.0%
!! ATAAATAAAATTAACAACAC+TGG - Chr8:33726248-33726267 MsG0880043979.01.T01:intron 15.0%
!! ATGATTACAAACTATTAAAA+TGG + Chr8:33726014-33726033 None:intergenic 15.0%
!! GTAAGAATTATGAATTATTT+GGG - Chr8:33726666-33726685 MsG0880043979.01.T01:intron 15.0%
!! TCATAAAATATGTCAATAAT+TGG - Chr8:33726211-33726230 MsG0880043979.01.T01:intron 15.0%
!! TCATTAGAAAAAATATTCAT+TGG - Chr8:33726762-33726781 MsG0880043979.01.T01:intron 15.0%
!! TGTAAGAATTATGAATTATT+TGG - Chr8:33726665-33726684 MsG0880043979.01.T01:intron 15.0%
!!! AATATAGTAGTTTTCTTTTT+AGG - Chr8:33726797-33726816 MsG0880043979.01.T01:intron 15.0%
!!! AATTTTATTTATGTTGTACT+GGG + Chr8:33726240-33726259 None:intergenic 15.0%
!!! ATATAGTAGTTTTCTTTTTA+GGG - Chr8:33726798-33726817 MsG0880043979.01.T01:intron 15.0%
!!! TAATTTTATTTATGTTGTAC+TGG + Chr8:33726241-33726260 None:intergenic 15.0%
!!! TGTAGTTTTTTTTTTTTTTG+TGG + Chr8:33726433-33726452 None:intergenic 15.0%
!!! TTATATCGATTATTTATTAC+TGG + Chr8:33726297-33726316 None:intergenic 15.0%
!! AATAATCCTCTATAGATATA+AGG - Chr8:33726075-33726094 MsG0880043979.01.T01:intron 20.0%
!!! AGTTTTTTTTTTTTTTGTGG+TGG + Chr8:33726430-33726449 None:intergenic 20.0%
!!! CTTTTGATCTAAAAGATATA+AGG - Chr8:33725786-33725805 MsG0880043979.01.T01:CDS 20.0%
!!! TTAAAGAGATTTGATTTTGT+AGG - Chr8:33725953-33725972 MsG0880043979.01.T01:intron 20.0%
! AAAAACACTTATAAGCTCTT+TGG - Chr8:33726996-33727015 MsG0880043979.01.T01:intron 25.0%
! AAAATGTCGAAAATAATCCT+AGG - Chr8:33726142-33726161 MsG0880043979.01.T01:intron 25.0%
! AATATGTTACTGTAGTTGTT+AGG + Chr8:33725736-33725755 None:intergenic 25.0%
! AGATATTGATGAGCATTATA+GGG - Chr8:33727022-33727041 MsG0880043979.01.T01:intron 25.0%
! ATCCTTGAAGTAATTGATAA+GGG - Chr8:33727056-33727075 MsG0880043979.01.T01:intron 25.0%
! TGATCTAAAAGATATAAGGT+TGG - Chr8:33725790-33725809 MsG0880043979.01.T01:CDS 25.0%
!! TAGTGATATTTTACCATTGT+CGG + Chr8:33726459-33726478 None:intergenic 25.0%
AGATTGTAGAACTTATCAGA+GGG + Chr8:33725591-33725610 None:intergenic 30.0%
AGTCAACCTTATATCTATAG+AGG + Chr8:33726084-33726103 None:intergenic 30.0%
CATCCTTGAAGTAATTGATA+AGG - Chr8:33727055-33727074 MsG0880043979.01.T01:intron 30.0%
CGAAAATAATCCTAGGTATA+AGG - Chr8:33726149-33726168 MsG0880043979.01.T01:intron 30.0%
CGACATAATAACATTTGCAA+AGG - Chr8:33725980-33725999 MsG0880043979.01.T01:intron 30.0%
CTTCGTAAACTTATCTAAGT+TGG - Chr8:33726358-33726377 MsG0880043979.01.T01:intron 30.0%
GAGATATTGATGAGCATTAT+AGG - Chr8:33727021-33727040 MsG0880043979.01.T01:intron 30.0%
TAGATTGTAGAACTTATCAG+AGG + Chr8:33725592-33725611 None:intergenic 30.0%
TTCAAAAGAGTCATAAGTGA+AGG + Chr8:33725845-33725864 None:intergenic 30.0%
! GTTTGTTGAATGTTAGTTTG+CGG + Chr8:33725922-33725941 None:intergenic 30.0%
! TCACTTATGACTCTTTTGAA+CGG - Chr8:33725845-33725864 MsG0880043979.01.T01:intron 30.0%
!! AAAATTAACAACACTGGCTT+TGG - Chr8:33726254-33726273 MsG0880043979.01.T01:intron 30.0%
!! AGAGATTTGATTTTGTAGGA+AGG - Chr8:33725957-33725976 MsG0880043979.01.T01:intron 30.0%
!! ATAGATATAAGGTTGACTTG+TGG - Chr8:33726086-33726105 MsG0880043979.01.T01:intron 30.0%
!! TTTGTTGAATGTTAGTTTGC+GGG + Chr8:33725921-33725940 None:intergenic 30.0%
!!! TTTTTTTTTTTTGTGGTGGC+CGG + Chr8:33726426-33726445 None:intergenic 30.0%
AAAAACTACACAACCGACAA+TGG - Chr8:33726443-33726462 MsG0880043979.01.T01:intron 35.0%
AAAAGAGTCATAAGTGAAGG+AGG + Chr8:33725842-33725861 None:intergenic 35.0%
AAATGTTCCATGTAGCCATT+TGG + Chr8:33727146-33727165 None:intergenic 35.0%
AACACTTATAAGCTCTTTGG+TGG - Chr8:33726999-33727018 MsG0880043979.01.T01:intron 35.0%
AACTTCATGAAGGTGAAGAT+TGG - Chr8:33725667-33725686 MsG0880043979.01.T01:CDS 35.0%
ACAAGTCAAACCTTATACCT+AGG + Chr8:33726162-33726181 None:intergenic 35.0%
ATAATTCACAACAGATGGCA+AGG - Chr8:33726926-33726945 MsG0880043979.01.T01:intron 35.0%
ATCTTCACCTTCATGAAGTT+TGG + Chr8:33725667-33725686 None:intergenic 35.0%
CACCCTTATCAATTACTTCA+AGG + Chr8:33727061-33727080 None:intergenic 35.0%
CATCATACCAAACTTCATGA+AGG - Chr8:33725657-33725676 MsG0880043979.01.T01:CDS 35.0%
CCATCATAATTCACAACAGA+TGG - Chr8:33726921-33726940 MsG0880043979.01.T01:intron 35.0%
CCATCTGTTGTGAATTATGA+TGG + Chr8:33726924-33726943 None:intergenic 35.0%
CTTGAAAATCTCATGCCAAA+TGG - Chr8:33725894-33725913 MsG0880043979.01.T01:intron 35.0%
GAAGTAATTGATAAGGGTGA+TGG - Chr8:33727062-33727081 MsG0880043979.01.T01:intron 35.0%
TGAGCATTATAGGGTCTTTA+AGG - Chr8:33727031-33727050 MsG0880043979.01.T01:intron 35.0%
TGTTGTGAATTATGATGGCA+CGG + Chr8:33726919-33726938 None:intergenic 35.0%
! AATGGCTACATGGAACATTT+TGG - Chr8:33727146-33727165 MsG0880043979.01.T01:CDS 35.0%
!! ATATAAGGTTGACTTGTGGT+TGG - Chr8:33726090-33726109 MsG0880043979.01.T01:intron 35.0%
!! TAGGTATAAGGTTTGACTTG+TGG - Chr8:33726161-33726180 MsG0880043979.01.T01:intron 35.0%
!! TATAAGGTTTGACTTGTGGT+TGG - Chr8:33726165-33726184 MsG0880043979.01.T01:intron 35.0%
!! TTTGGCATGAGATTTTCAAG+TGG + Chr8:33725894-33725913 None:intergenic 35.0%
!!! TTTTTTTTTTTGTGGTGGCC+GGG + Chr8:33726425-33726444 None:intergenic 35.0%
ATTTGCGCCTTTGACAAGAT+GGG + Chr8:33727195-33727214 None:intergenic 40.0%
CATTATACATGCAACGTCCA+GGG - Chr8:33726392-33726411 MsG0880043979.01.T01:intron 40.0%
GTTGTGAATTATGATGGCAC+GGG + Chr8:33726918-33726937 None:intergenic 40.0%
TAAGTGAAGGAGGTGATTGA+AGG + Chr8:33725832-33725851 None:intergenic 40.0%
TATTTGCGCCTTTGACAAGA+TGG + Chr8:33727196-33727215 None:intergenic 40.0%
! GGTAAGCTCAGTACTGAAAT+TGG - Chr8:33725555-33725574 MsG0880043979.01.T01:CDS 40.0%
!!! GCATGAGATTTTCAAGTGGA+AGG + Chr8:33725890-33725909 None:intergenic 40.0%
!!! TTTTTTTTTTGTGGTGGCCG+GGG + Chr8:33726424-33726443 None:intergenic 40.0%
GAGCATATTGACCCTCCAAA+TGG - Chr8:33727128-33727147 MsG0880043979.01.T01:CDS 45.0%
GCATTATACATGCAACGTCC+AGG - Chr8:33726391-33726410 MsG0880043979.01.T01:intron 45.0%
GTTCCATGTAGCCATTTGGA+GGG + Chr8:33727142-33727161 None:intergenic 45.0%
GTTGATGCCCATCTTGTCAA+AGG - Chr8:33727185-33727204 MsG0880043979.01.T01:CDS 45.0%
TGAAGGAGGTGATTGAAGGA+GGG + Chr8:33725828-33725847 None:intergenic 45.0%
TGTTCCATGTAGCCATTTGG+AGG + Chr8:33727143-33727162 None:intergenic 45.0%
!! TGTTAGTTTGCGGGACCATT+TGG + Chr8:33725912-33725931 None:intergenic 45.0%
!!! ACAATAAATATTTAATTTAT+AGG + Chr8:33726733-33726752 None:intergenic 5.0%
ATGGCACTGCTGCTGCTAAA+TGG - Chr8:33727081-33727100 MsG0880043979.01.T01:intron 50.0%
GAGGTGATTGAAGGAGGGAT+AGG + Chr8:33725823-33725842 None:intergenic 50.0%
GTGAAGGAGGTGATTGAAGG+AGG + Chr8:33725829-33725848 None:intergenic 50.0%
TGACCCTCCAAATGGCTACA+TGG - Chr8:33727136-33727155 MsG0880043979.01.T01:CDS 50.0%
AACGTCCAGGGTTCAAACCC+CGG - Chr8:33726404-33726423 MsG0880043979.01.T01:intron 55.0%
GGTGGCCGGGGTTTGAACCC+TGG + Chr8:33726412-33726431 None:intergenic 70.0%
Chromosome Type Strat End Strand Name
Chr8 gene 33725543 33727226 33725543 ID=MsG0880043979.01;Name=MsG0880043979.01
Chr8 mRNA 33725543 33727226 33725543 ID=MsG0880043979.01.T01;Parent=MsG0880043979.01;Name=MsG0880043979.01.T01;_AED=0.50;_eAED=0.51;_QI=0|0|0|1|1|1|2|0|140
Chr8 exon 33727091 33727226 33727091 ID=MsG0880043979.01.T01:exon:10244;Parent=MsG0880043979.01.T01
Chr8 exon 33725543 33725829 33725543 ID=MsG0880043979.01.T01:exon:10243;Parent=MsG0880043979.01.T01
Chr8 CDS 33727091 33727226 33727091 ID=MsG0880043979.01.T01:cds;Parent=MsG0880043979.01.T01
Chr8 CDS 33725543 33725829 33725543 ID=MsG0880043979.01.T01:cds;Parent=MsG0880043979.01.T01
Gene Sequence

>MsG0880043979.01.T01

ATGGTGCTCTCTGGTAAGCTCAGTACTGAAATTGGAATCAAAACACCCTCTGATAAGTTCTACAATCTATTTGTAACAGAACTTCATGAAGTGCAAAACATTAGTGAAAGAGTTCATCATACCAAACTTCATGAAGATGGCAAGGTACACACATGTCATGAGAGTGTTGAAGAAGTTGATGAACTGAATAAAAAAAACACTTATAAGCTCTTTGGTGGAGATATTGATGAGCATTATAGGGTCTTTAAGGTCATCCTTGAAGTAATTGATAAGGGTGATGGCACTGCTGCTGCTAAATGGACAGTTGAATATGAAAAGATCAATGAGCATATTGACCCTCCAAATGGCTACATGGAACATTTTGGCAAAGTCACTAGAGATGTTGATGCCCATCTTGTCAAAGGCGCAAATATAGCTCAATAA

Protein sequence

>MsG0880043979.01.T01

MVLSGKLSTEIGIKTPSDKFYNLFVTELHEVQNISERVHHTKLHEDGKVHTCHESVEEVDELNKKNTYKLFGGDIDEHYRVFKVILEVIDKGDGTAAAKWTVEYEKINEHIDPPNGYMEHFGKVTRDVDAHLVKGANIAQ*