AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar ZhongmuNo.1 / MsG0280010489.01


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsG0280010489.01.T01 MTR_2g084985 99.099 111 1 0 1 111 1 111 6.25e-75 217
MsG0280010489.01.T01 MTR_4g124920 41.584 101 55 2 8 105 4 103 1.45e-21 82.4
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsG0280010489.01.T01 AT5G22580 65.657 99 34 0 7 105 6 104 5.51e-42 134
MsG0280010489.01.T01 AT3G17210 42.000 100 54 2 8 104 9 107 3.51e-22 84.0
MsG0280010489.01.T01 AT3G17210 42.000 100 54 2 8 104 9 107 3.51e-22 84.0

Find 34 sgRNAs with CRISPR-Local

Find 64 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
GATTATGCTGCTTCCTTAAC+TGG 0.195704 2:+70451209 None:intergenic
TACAAAAGGGATGGAGAAAT+TGG 0.322091 2:-70452047 MsG0280010489.01.T01:CDS
AATCATATCCATTGATTAGA+AGG 0.325835 2:+70451016 None:intergenic
AATAATGAATTATTTATTGT+TGG 0.345498 2:+70451171 None:intergenic
AAGCTTTACTTCACAAAGAA+TGG 0.408647 2:-70452137 None:intergenic
ATTTGTATGGTAAAAGATAA+GGG 0.415631 2:-70451043 MsG0280010489.01.T01:three_prime_UTR
AGGGTTTGCCTTCTAATCAA+TGG 0.422168 2:-70451024 MsG0280010489.01.T01:three_prime_UTR
ATCTTGTCAAATCCTTTGAA+TGG 0.422994 2:-70452012 MsG0280010489.01.T01:intron
AACTGGCTTGACAAGGGTTG+AGG 0.423667 2:+70451226 None:intergenic
AACCCTTGTCAAGCCAGTTA+AGG 0.431460 2:-70451222 MsG0280010489.01.T01:CDS
TTTGTACTGTGATATTTGTA+TGG 0.444698 2:-70451056 MsG0280010489.01.T01:three_prime_UTR
CTTCCTTAACTGGCTTGACA+AGG 0.454173 2:+70451219 None:intergenic
CTTGTTGAATGTCATCAAGA+AGG 0.457412 2:+70451346 None:intergenic
GTGATTGTTAAGTTCAAAGA+AGG 0.458164 2:-70452088 MsG0280010489.01.T01:CDS
TATTTGTATGGTAAAAGATA+AGG 0.471222 2:-70451044 MsG0280010489.01.T01:three_prime_UTR
GTAGTGGAAGAGCTTACAAA+AGG 0.480357 2:-70452061 MsG0280010489.01.T01:CDS
GTTCAAAGAAGGTGTAGTAG+TGG 0.481023 2:-70452077 MsG0280010489.01.T01:CDS
GGGACAGGATATTGAAAGCC+TGG 0.493701 2:-70451396 MsG0280010489.01.T01:CDS
TCAGAGAACTCAACATGACT+TGG 0.494285 2:+70451293 None:intergenic
AACCTTGTCTCAACACATCC+AGG 0.501843 2:+70451378 None:intergenic
ACTGGCTTGACAAGGGTTGA+GGG 0.505995 2:+70451227 None:intergenic
AGAACTCAACATGACTTGGA+TGG 0.554383 2:+70451297 None:intergenic
GTATGTCTTATACAGGGGAC+AGG 0.555403 2:-70451411 MsG0280010489.01.T01:intron
TTCACAAAGAATGGTAGTCA+TGG 0.559784 2:-70452128 MsG0280010489.01.T01:exon
TTCCTTAACTGGCTTGACAA+GGG 0.564730 2:+70451220 None:intergenic
CTGGCTTGACAAGGGTTGAG+GGG 0.581401 2:+70451228 None:intergenic
GTATAAAACATACCATTCAA+AGG 0.584418 2:+70452000 None:intergenic
TAGTGGAAGAGCTTACAAAA+GGG 0.588758 2:-70452060 MsG0280010489.01.T01:CDS
GGAAGAGCTTACAAAAGGGA+TGG 0.589362 2:-70452056 MsG0280010489.01.T01:CDS
ACAACTAAACTACAAACAGA+AGG 0.613840 2:+70451102 None:intergenic
TGAATGTCATCAAGAAGGCA+TGG 0.625633 2:+70451351 None:intergenic
AGCCTGGATGTGTTGAGACA+AGG 0.635747 2:-70451380 MsG0280010489.01.T01:CDS
ACAAAGAATGGTAGTCATGG+AGG 0.636423 2:-70452125 MsG0280010489.01.T01:exon
GAATGTCATCAAGAAGGCAT+GGG 0.659643 2:+70451352 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! AATAATGAATTATTTATTGT+TGG + Chr2:70451968-70451987 None:intergenic 10.0%
!!! ACATCAAAAAATTTGATTTT+GGG - Chr2:70451456-70451475 MsG0280010489.01.T01:intron 15.0%
!! AACAAAGACAGTAATTTAAA+GGG - Chr2:70451336-70451355 MsG0280010489.01.T01:CDS 20.0%
!! ATTTGTATGGTAAAAGATAA+GGG - Chr2:70452093-70452112 MsG0280010489.01.T01:CDS 20.0%
!! TATTTGTATGGTAAAAGATA+AGG - Chr2:70452092-70452111 MsG0280010489.01.T01:CDS 20.0%
!!! GACATCAAAAAATTTGATTT+TGG - Chr2:70451455-70451474 MsG0280010489.01.T01:intron 20.0%
! AAAAACTATCAGTATCTATG+AGG + Chr2:70451254-70451273 None:intergenic 25.0%
! AACAGAAACAGAAATAACAA+GGG - Chr2:70451303-70451322 MsG0280010489.01.T01:CDS 25.0%
! AATCAAGTATGTCTTATACA+GGG - Chr2:70451719-70451738 MsG0280010489.01.T01:intron 25.0%
! ACTGAGATTTATAGCTAAAT+AGG - Chr2:70451402-70451421 MsG0280010489.01.T01:intron 25.0%
! ATGATATGATAGTACTAAGT+AGG + Chr2:70451690-70451709 None:intergenic 25.0%
! ATGTGACGATTATGATTATT+TGG - Chr2:70451638-70451657 MsG0280010489.01.T01:intron 25.0%
! GAACAAAGACAGTAATTTAA+AGG - Chr2:70451335-70451354 MsG0280010489.01.T01:CDS 25.0%
! GTATAAAACATACCATTCAA+AGG + Chr2:70451139-70451158 None:intergenic 25.0%
! TAACAGAAACAGAAATAACA+AGG - Chr2:70451302-70451321 MsG0280010489.01.T01:CDS 25.0%
! TAATCAAGTATGTCTTATAC+AGG - Chr2:70451718-70451737 MsG0280010489.01.T01:intron 25.0%
! TGATATGATAGTACTAAGTA+GGG + Chr2:70451689-70451708 None:intergenic 25.0%
! TTTGTACTGTGATATTTGTA+TGG - Chr2:70452080-70452099 MsG0280010489.01.T01:CDS 25.0%
!! AATCATATCCATTGATTAGA+AGG + Chr2:70452123-70452142 None:intergenic 25.0%
!! TACCTTTTATATTATGCAAG+AGG - Chr2:70451162-70451181 MsG0280010489.01.T01:three_prime_UTR 25.0%
ACAACTAAACTACAAACAGA+AGG + Chr2:70452037-70452056 None:intergenic 30.0%
ATCAAGTATGTCTTATACAG+GGG - Chr2:70451720-70451739 MsG0280010489.01.T01:intron 30.0%
ATCTTGTCAAATCCTTTGAA+TGG - Chr2:70451124-70451143 MsG0280010489.01.T01:three_prime_UTR 30.0%
CTATGAGGTTAATAAACTGA+AGG + Chr2:70451239-70451258 None:intergenic 30.0%
GTGATTGTTAAGTTCAAAGA+AGG - Chr2:70451048-70451067 MsG0280010489.01.T01:three_prime_UTR 30.0%
TGCCTCTTGCATAATATAAA+AGG + Chr2:70451167-70451186 None:intergenic 30.0%
CTTGTTGAATGTCATCAAGA+AGG + Chr2:70451793-70451812 None:intergenic 35.0%
TACAAAAGGGATGGAGAAAT+TGG - Chr2:70451089-70451108 MsG0280010489.01.T01:three_prime_UTR 35.0%
TAGTGGAAGAGCTTACAAAA+GGG - Chr2:70451076-70451095 MsG0280010489.01.T01:three_prime_UTR 35.0%
TTCACAAAGAATGGTAGTCA+TGG - Chr2:70451008-70451027 MsG0280010489.01.T01:three_prime_UTR 35.0%
! GTATACGATTAGTTGTGACT+TGG + Chr2:70451574-70451593 None:intergenic 35.0%
! TTGATTTTGGGCTTACATCA+CGG - Chr2:70451468-70451487 MsG0280010489.01.T01:intron 35.0%
!!! ATTTTTTGATGTCTTCGCGT+TGG + Chr2:70451448-70451467 None:intergenic 35.0%
ACAAAGAATGGTAGTCATGG+AGG - Chr2:70451011-70451030 MsG0280010489.01.T01:three_prime_UTR 40.0%
AGGGTTTGCCTTCTAATCAA+TGG - Chr2:70452112-70452131 MsG0280010489.01.T01:CDS 40.0%
TCAGAGAACTCAACATGACT+TGG + Chr2:70451846-70451865 None:intergenic 40.0%
TGAATGTCATCAAGAAGGCA+TGG + Chr2:70451788-70451807 None:intergenic 40.0%
! AGAACTCAACATGACTTGGA+TGG + Chr2:70451842-70451861 None:intergenic 40.0%
! GAATGTCATCAAGAAGGCAT+GGG + Chr2:70451787-70451806 None:intergenic 40.0%
! GATTATGCTGCTTCCTTAAC+TGG + Chr2:70451930-70451949 None:intergenic 40.0%
! GTAGTGGAAGAGCTTACAAA+AGG - Chr2:70451075-70451094 MsG0280010489.01.T01:three_prime_UTR 40.0%
! GTTCAAAGAAGGTGTAGTAG+TGG - Chr2:70451059-70451078 MsG0280010489.01.T01:three_prime_UTR 40.0%
!! GATTAGTTGTGACTTGGTGT+CGG + Chr2:70451568-70451587 None:intergenic 40.0%
!! TTCCTTAACTGGCTTGACAA+GGG + Chr2:70451919-70451938 None:intergenic 40.0%
AACCCTTGTCAAGCCAGTTA+AGG - Chr2:70451914-70451933 MsG0280010489.01.T01:intron 45.0%
AACCTTGTCTCAACACATCC+AGG + Chr2:70451761-70451780 None:intergenic 45.0%
GGAAGAGCTTACAAAAGGGA+TGG - Chr2:70451080-70451099 MsG0280010489.01.T01:three_prime_UTR 45.0%
GTATGTCTTATACAGGGGAC+AGG - Chr2:70451725-70451744 MsG0280010489.01.T01:intron 45.0%
!! AGCTAAATAGGCGTCGATTG+CGG - Chr2:70451414-70451433 MsG0280010489.01.T01:intron 45.0%
!! ATGTCTTCGCGTTGGTAATG+TGG + Chr2:70451440-70451459 None:intergenic 45.0%
!! CTTCCTTAACTGGCTTGACA+AGG + Chr2:70451920-70451939 None:intergenic 45.0%
AAGGGAAATTCCCCAACCAG+AGG - Chr2:70451354-70451373 MsG0280010489.01.T01:CDS 50.0%
AATTCCCCAACCAGAGGTTG+CGG - Chr2:70451360-70451379 MsG0280010489.01.T01:CDS 50.0%
AGAGGTTGCGGTCATGTTCA+CGG - Chr2:70451372-70451391 MsG0280010489.01.T01:CDS 50.0%
AGCCTGGATGTGTTGAGACA+AGG - Chr2:70451756-70451775 MsG0280010489.01.T01:intron 50.0%
CGGTCGTAGACTCGTAGTTA+TGG - Chr2:70451488-70451507 MsG0280010489.01.T01:intron 50.0%
GGGACAGGATATTGAAAGCC+TGG - Chr2:70451740-70451759 MsG0280010489.01.T01:intron 50.0%
!! AACTGGCTTGACAAGGGTTG+AGG + Chr2:70451913-70451932 None:intergenic 50.0%
!! ACTGGCTTGACAAGGGTTGA+GGG + Chr2:70451912-70451931 None:intergenic 50.0%
ACATGACCGCAACCTCTGGT+TGG + Chr2:70451369-70451388 None:intergenic 55.0%
ATGACCGCAACCTCTGGTTG+GGG + Chr2:70451367-70451386 None:intergenic 55.0%
CATGACCGCAACCTCTGGTT+GGG + Chr2:70451368-70451387 None:intergenic 55.0%
GTGAACATGACCGCAACCTC+TGG + Chr2:70451373-70451392 None:intergenic 55.0%
! CTGGCTTGACAAGGGTTGAG+GGG + Chr2:70451911-70451930 None:intergenic 55.0%
Chromosome Type Strat End Strand Name
Chr2 gene 70451006 70452152 70451006 ID=MsG0280010489.01;Name=MsG0280010489.01
Chr2 mRNA 70451006 70452152 70451006 ID=MsG0280010489.01.T01;Parent=MsG0280010489.01;Name=MsG0280010489.01.T01;_AED=0.28;_eAED=0.28;_QI=12|1|1|1|1|1|2|205|111
Chr2 exon 70451006 70451418 70451006 ID=MsG0280010489.01.T01:exon:10006;Parent=MsG0280010489.01.T01
Chr2 exon 70452013 70452152 70452013 ID=MsG0280010489.01.T01:exon:10005;Parent=MsG0280010489.01.T01
Chr2 five_prime_UTR 70452141 70452152 70452141 ID=MsG0280010489.01.T01:five_prime_utr;Parent=MsG0280010489.01.T01
Chr2 CDS 70452013 70452140 70452013 ID=MsG0280010489.01.T01:cds;Parent=MsG0280010489.01.T01
Chr2 CDS 70451211 70451418 70451211 ID=MsG0280010489.01.T01:cds;Parent=MsG0280010489.01.T01
Chr2 three_prime_UTR 70451006 70451210 70451006 ID=MsG0280010489.01.T01:three_prime_utr;Parent=MsG0280010489.01.T01
Gene Sequence

>MsG0280010489.01.T01

ATGGTAGTCATGGAGGAATTCAAGCACTTTGTGATTGTTAAGTTCAAAGAAGGTGTAGTAGTGGAAGAGCTTACAAAAGGGATGGAGAAATTGGTCTCAGAGATTGATCTTGTCAAATCCTTTGAATGGGGACAGGATATTGAAAGCCTGGATGTGTTGAGACAAGGTTTCACCCATGCCTTCTTGATGACATTCAACAAGAAAGAAGATTTTGCTGCATTTCAGAGCCATCCAAGTCATGTTGAGTTCTCTGAAAAGTTTTCAACAGCTATTGAAAAGATTGTGCTGCTTGATTTCCCCTCAACCCTTGTCAAGCCAGTTAAGGAAGCAGCATAA

Protein sequence

>MsG0280010489.01.T01

MVVMEEFKHFVIVKFKEGVVVEELTKGMEKLVSEIDLVKSFEWGQDIESLDVLRQGFTHAFLMTFNKKEDFAAFQSHPSHVEFSEKFSTAIEKIVLLDFPSTLVKPVKEAA*