AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar ZhongmuNo.1 / MsG0480022239.01


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsG0480022239.01.T01 MTR_4g082900 71.605 81 22 1 1 80 1 81 1.11e-25 90.9
MsG0480022239.01.T01 MTR_4g082883 54.667 75 31 2 8 80 26 99 3.21e-16 67.4
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score

Find 15 sgRNAs with CRISPR-Local

Find 22 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
GGCTGGACCTAACGATCTTT+TGG 0.317659 4:-70036714 MsG0480022239.01.T01:CDS
TTTGCCAATGGATCTGGTTT+GGG 0.342149 4:+70036549 None:intergenic
GTACTTGCCAAAAGATCGTT+AGG 0.439536 4:+70036707 None:intergenic
TAAACCCAAACCAGATCCAT+TGG 0.516853 4:-70036553 MsG0480022239.01.T01:CDS
GATGATGCTACAAAGTCTGC+TGG 0.547087 4:-70036512 MsG0480022239.01.T01:CDS
ACTGTAGGAAATCTTGCAGC+TGG 0.550456 4:-70036656 MsG0480022239.01.T01:CDS
GCTGGTGTTCATAAGACTGT+AGG 0.573068 4:-70036605 MsG0480022239.01.T01:CDS
GCATCAGCACAGAAAACTGT+AGG 0.588939 4:-70036671 MsG0480022239.01.T01:CDS
GTAGGAAATCTTGCAGCTGG+TGG 0.593110 4:-70036653 MsG0480022239.01.T01:CDS
GTTTACAAAATCAGCAGCGT+TGG 0.603118 4:+70036571 None:intergenic
TAGCATCATCAACCGCACCT+TGG 0.613058 4:+70036525 None:intergenic
AATGTGGGAAATGCTGCAGC+TGG 0.619200 4:-70036623 MsG0480022239.01.T01:CDS
CAGATCCATTGGCAAAACCA+AGG 0.650829 4:-70036542 MsG0480022239.01.T01:CDS
CCATTGGCAAAACCAAGGTG+CGG 0.698184 4:-70036537 MsG0480022239.01.T01:CDS
GATGCTACAAAGTCTGCTGG+AGG 0.702868 4:-70036509 MsG0480022239.01.T01:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
GTACTTGCCAAAAGATCGTT+AGG + Chr4:70036508-70036527 None:intergenic 40.0%
GTTTACAAAATCAGCAGCGT+TGG + Chr4:70036644-70036663 None:intergenic 40.0%
TAAACCCAAACCAGATCCAT+TGG - Chr4:70036659-70036678 MsG0480022239.01.T01:CDS 40.0%
! CAGCATTTCCCACATTTTTC+TGG + Chr4:70036585-70036604 None:intergenic 40.0%
! CGATCTTTTGGCAAGTACAA+AGG - Chr4:70036510-70036529 MsG0480022239.01.T01:CDS 40.0%
! TTTGCCAATGGATCTGGTTT+GGG + Chr4:70036666-70036685 None:intergenic 40.0%
! TTTTGCCAATGGATCTGGTT+TGG + Chr4:70036667-70036686 None:intergenic 40.0%
ACTGTAGGAAATCTTGCAGC+TGG - Chr4:70036556-70036575 MsG0480022239.01.T01:CDS 45.0%
CAGATCCATTGGCAAAACCA+AGG - Chr4:70036670-70036689 MsG0480022239.01.T01:CDS 45.0%
GATGATGCTACAAAGTCTGC+TGG - Chr4:70036700-70036719 MsG0480022239.01.T01:CDS 45.0%
GCATCAGCACAGAAAACTGT+AGG - Chr4:70036541-70036560 MsG0480022239.01.T01:CDS 45.0%
GCTGGTGTTCATAAGACTGT+AGG - Chr4:70036607-70036626 MsG0480022239.01.T01:CDS 45.0%
!!! CTTGGTTTTGCCAATGGATC+TGG + Chr4:70036672-70036691 None:intergenic 45.0%
AATGTGGGAAATGCTGCAGC+TGG - Chr4:70036589-70036608 MsG0480022239.01.T01:CDS 50.0%
AGCTGGTGGCCAGAAAAATG+TGG - Chr4:70036573-70036592 MsG0480022239.01.T01:CDS 50.0%
CCATTGGCAAAACCAAGGTG+CGG - Chr4:70036675-70036694 MsG0480022239.01.T01:CDS 50.0%
GATGCTACAAAGTCTGCTGG+AGG - Chr4:70036703-70036722 MsG0480022239.01.T01:CDS 50.0%
GCTGGTGGCCAGAAAAATGT+GGG - Chr4:70036574-70036593 MsG0480022239.01.T01:CDS 50.0%
GTAGGAAATCTTGCAGCTGG+TGG - Chr4:70036559-70036578 MsG0480022239.01.T01:CDS 50.0%
TAGCATCATCAACCGCACCT+TGG + Chr4:70036690-70036709 None:intergenic 50.0%
! GGCTGGACCTAACGATCTTT+TGG - Chr4:70036498-70036517 MsG0480022239.01.T01:CDS 50.0%
!!! CCGCACCTTGGTTTTGCCAA+TGG + Chr4:70036678-70036697 None:intergenic 55.0%
Chromosome Type Strat End Strand Name
Chr4 gene 70036496 70036738 70036496 ID=MsG0480022239.01;Name=MsG0480022239.01
Chr4 mRNA 70036496 70036738 70036496 ID=MsG0480022239.01.T01;Parent=MsG0480022239.01;Name=MsG0480022239.01.T01;_AED=0.50;_eAED=0.50;_QI=0|-1|0|1|-1|1|1|0|80
Chr4 exon 70036496 70036738 70036496 ID=MsG0480022239.01.T01:exon:11097;Parent=MsG0480022239.01.T01
Chr4 CDS 70036496 70036738 70036496 ID=MsG0480022239.01.T01:cds;Parent=MsG0480022239.01.T01
Gene Sequence

>MsG0480022239.01.T01

ATGGCTGGACCTAACGATCTTTTGGCAAGTACAAAGGATGCAGCAGCATCAGCACAGAAAACTGTAGGAAATCTTGCAGCTGGTGGCCAGAAAAATGTGGGAAATGCTGCAGCTGGTGTTCATAAGACTGTAGGAGATTTTGCTACCAACGCTGCTGATTTTGTAAACCCAAACCAGATCCATTGGCAAAACCAAGGTGCGGTTGATGATGCTACAAAGTCTGCTGGAGGCTTATTTAAATGA

Protein sequence

>MsG0480022239.01.T01

MAGPNDLLASTKDAAASAQKTVGNLAAGGQKNVGNAAAGVHKTVGDFATNAADFVNPNQIHWQNQGAVDDATKSAGGLFK*