AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar ZhongmuNo.1 / MsG0880044550.01

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsG0880044550.01.T01 MTR_8g464460 75.758 198 29 4 5 200 26 206 4.39e-99 288
MsG0880044550.01.T01 MTR_2g060830 70.707 198 39 4 5 200 26 206 9.78e-92 269
MsG0880044550.01.T01 MTR_7g070070 34.536 194 109 4 5 198 22 197 3.36e-23 94.0
MsG0880044550.01.T01 MTR_5g016590 34.021 194 110 4 5 198 22 197 4.97e-22 90.9
MsG0880044550.01.T01 MTR_0167s0060 33.149 181 103 4 1 181 19 181 1.40e-21 89.4
MsG0880044550.01.T01 MTR_8g075320 30.114 176 105 4 5 179 21 179 8.86e-16 73.9
MsG0880044550.01.T01 MTR_1g022410 32.386 176 99 4 5 180 23 178 2.19e-15 73.2
MsG0880044550.01.T01 MTR_2g016050 29.944 177 106 4 5 180 21 180 3.12e-15 72.4
MsG0880044550.01.T01 MTR_1g112250 28.571 189 114 6 5 190 25 195 6.34e-15 71.2
MsG0880044550.01.T01 MTR_6g024135 30.058 173 101 4 5 176 25 178 8.11e-13 65.9
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsG0880044550.01.T01 AT2G05840 66.667 198 46 3 5 200 26 205 2.68e-88 259
MsG0880044550.01.T01 AT2G05840 65.657 198 49 4 5 200 26 206 1.86e-86 256
MsG0880044550.01.T01 AT2G05840 67.196 189 44 3 5 192 26 197 3.63e-86 254
MsG0880044550.01.T01 AT5G35590 67.196 189 42 3 5 191 26 196 6.68e-86 254
MsG0880044550.01.T01 AT1G16470 33.149 181 103 4 1 181 19 181 6.26e-21 87.4
MsG0880044550.01.T01 AT1G16470 33.149 181 103 4 1 181 19 181 6.26e-21 87.4
MsG0880044550.01.T01 AT1G79210 33.149 181 103 4 1 181 19 181 1.15e-20 86.7
MsG0880044550.01.T01 AT1G79210 33.149 181 103 4 1 181 19 181 1.15e-20 86.7
MsG0880044550.01.T01 AT1G79210 33.149 181 103 4 1 181 19 181 1.15e-20 86.7
MsG0880044550.01.T01 AT3G22110 32.474 194 113 4 5 198 22 197 1.42e-20 87.0
MsG0880044550.01.T01 AT1G53850 29.843 191 113 6 5 192 25 197 4.03e-16 74.7
MsG0880044550.01.T01 AT1G53850 29.843 191 113 6 5 192 25 197 4.03e-16 74.7
MsG0880044550.01.T01 AT5G66140 29.944 177 104 5 5 179 21 179 6.07e-16 74.3
MsG0880044550.01.T01 AT3G51260 30.114 176 105 4 5 179 21 179 6.09e-16 74.3
MsG0880044550.01.T01 AT3G14290 29.319 191 114 6 5 192 25 197 9.11e-16 73.6
MsG0880044550.01.T01 AT3G51260 29.500 200 119 6 5 202 21 200 9.69e-16 73.9
MsG0880044550.01.T01 AT1G47250 31.250 176 101 4 5 180 23 178 3.90e-14 69.7
MsG0880044550.01.T01 AT5G42790 30.682 176 102 4 5 180 23 178 8.56e-14 68.9
MsG0880044550.01.T01 AT2G27020 28.643 199 112 7 5 196 25 200 1.22e-11 62.4
MsG0880044550.01.T01 AT2G27020 29.775 178 99 6 5 175 127 285 2.12e-11 62.4

Find 56 sgRNAs with CRISPR-Local

Find 238 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
TACCAAGTACCTTGGTTTAC+TGG 0.220686 8:-44225291 MsG0880044550.01.T01:CDS
ACAAAATCTGACTTGCAAAA+TGG 0.225252 8:+44221614 None:intergenic
AGACGGTTCAGGTAATATTT+TGG 0.261296 8:-44221661 MsG0880044550.01.T01:CDS
GAGTGAAGTTGCTATGGTTC+TGG 0.269202 8:-44223420 MsG0880044550.01.T01:intron
GGCCACAAGTGCTGGATTAA+AGG 0.275884 8:-44221747 MsG0880044550.01.T01:CDS
TTGTGTGGTTACTCAGAAAA+AGG 0.276202 8:-44225452 MsG0880044550.01.T01:CDS
CCAAGAGGCAATCAATTTCT+TGG 0.284591 8:-44221723 MsG0880044550.01.T01:CDS
AAACCAAGGTACTTGGTAAT+AGG 0.326413 8:+44225296 None:intergenic
CTGTGGATGTGCTGTCTAAA+TGG 0.340867 8:-44224080 MsG0880044550.01.T01:intron
GGATAACCTCGATTGGTGTT+AGG 0.343827 8:-44225489 MsG0880044550.01.T01:CDS
CTGGGTATTGACGATGAACT+TGG 0.365337 8:-44223401 MsG0880044550.01.T01:CDS
CAAAATCTGACTTGCAAAAT+GGG 0.377090 8:+44221615 None:intergenic
TTCAAGGCTGTTAAGTCTGC+AGG 0.383896 8:-44225511 MsG0880044550.01.T01:CDS
CAGGGTCACATTTGTAAAGC+TGG 0.386432 8:+44223375 None:intergenic
TACCTATGCCGAGACGGTTC+AGG 0.388567 8:-44221672 MsG0880044550.01.T01:CDS
TTCAACAGAGTATGCTTTCA+AGG 0.399747 8:-44225527 MsG0880044550.01.T01:CDS
GATAACCTCGATTGGTGTTA+GGG 0.418833 8:-44225488 MsG0880044550.01.T01:CDS
CCAAGAAATTGATTGCCTCT+TGG 0.430454 8:+44221723 None:intergenic
TGTACTCAGGCCACAAGTGC+TGG 0.430811 8:-44221755 MsG0880044550.01.T01:intron
AGTGAAGTTGCTATGGTTCT+GGG 0.439091 8:-44223419 MsG0880044550.01.T01:intron
GTCCTTTAATCCAGCACTTG+TGG 0.454543 8:+44221745 None:intergenic
TGCTTGTTGAACTAGTGTCC+TGG 0.461152 8:+44224149 None:intergenic
GCTGAGTATCGCTTTAGATC+TGG 0.465040 8:-44224114 MsG0880044550.01.T01:CDS
GGCCAGTAAACCAAGGTACT+TGG 0.487125 8:+44225289 None:intergenic
GACCCTGCTGGTCATTACTT+TGG 0.498366 8:-44223359 MsG0880044550.01.T01:CDS
TACCTTGGTTTACTGGCCAC+TGG 0.499825 8:-44225284 MsG0880044550.01.T01:CDS
TAGGTGGGATTGTTTAATGC+AGG 0.501679 8:-44225357 MsG0880044550.01.T01:CDS
AGGGTCACATTTGTAAAGCT+GGG 0.502939 8:+44223376 None:intergenic
GTCTCGGCATAGGTAAATGA+AGG 0.506661 8:+44221680 None:intergenic
AGAGATGTGTAACACTTGTA+TGG 0.512075 8:+44225321 None:intergenic
TTACTGGCCACTGGCATCAC+AGG 0.515432 8:-44225275 MsG0880044550.01.T01:CDS
TTTCCTATTACCAAGTACCT+TGG 0.521924 8:-44225299 MsG0880044550.01.T01:CDS
TGACCAAAGTAATGACCAGC+AGG 0.522657 8:+44223356 None:intergenic
CATGCTTATATGAGACCACT+TGG 0.542442 8:-44223602 MsG0880044550.01.T01:CDS
TTCATTTACCTATGCCGAGA+CGG 0.554212 8:-44221678 MsG0880044550.01.T01:CDS
CTTTACAAATGTGACCCTGC+TGG 0.558651 8:-44223371 MsG0880044550.01.T01:CDS
GGGAAAAGATTCAGTTTGTG+TGG 0.559768 8:-44225467 MsG0880044550.01.T01:CDS
AAATATTACCTGAACCGTCT+CGG 0.561908 8:+44221664 None:intergenic
TGGGTATTGACGATGAACTT+GGG 0.570691 8:-44223400 MsG0880044550.01.T01:CDS
ATTGTGGAGTGAAGTTGCTA+TGG 0.574803 8:-44223426 MsG0880044550.01.T01:intron
TACCTGAACCGTCTCGGCAT+AGG 0.580613 8:+44221670 None:intergenic
CATTTAGACAGCACATCCAC+AGG 0.587968 8:+44224081 None:intergenic
TCTGCAGGGATAACCTCGAT+TGG 0.594530 8:-44225496 MsG0880044550.01.T01:CDS
TCAAGGCTGTTAAGTCTGCA+GGG 0.604847 8:-44225510 MsG0880044550.01.T01:CDS
TATATGAGACCACTTGGAGT+AGG 0.609958 8:-44223596 MsG0880044550.01.T01:intron
TGGTCATTACTTTGGTCACA+AGG 0.621200 8:-44223351 MsG0880044550.01.T01:intron
TGCTGGATTAAAGGACCAAG+AGG 0.623889 8:-44221738 MsG0880044550.01.T01:CDS
TGACTTGCAAAATGGGAGTG+AGG 0.631512 8:+44221622 None:intergenic
AAACACATACCTACTCCAAG+TGG 0.652321 8:+44223587 None:intergenic
GGGTCACATTTGTAAAGCTG+GGG 0.657277 8:+44223377 None:intergenic
TGCCAGTGGCCAGTAAACCA+AGG 0.682988 8:+44225282 None:intergenic
TCTCGGCATAGGTAAATGAA+GGG 0.686969 8:+44221681 None:intergenic
ATCTGGATATGAGATGCCTG+TGG 0.692452 8:-44224097 MsG0880044550.01.T01:CDS
ATAACCTCGATTGGTGTTAG+GGG 0.701420 8:-44225487 MsG0880044550.01.T01:CDS
GACCAAAGTAATGACCAGCA+GGG 0.719039 8:+44223357 None:intergenic
GAACATACCTGTGATGCCAG+TGG 0.749474 8:+44225268 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! TAAATTTAAATATATATATT+TGG + Chr8:44222759-44222778 None:intergenic 0.0%
!! AAATAATAAGAAGATAATTA+TGG - Chr8:44223209-44223228 MsG0880044550.01.T01:intron 10.0%
!! TTAAGATTCTTTATTAAATT+TGG - Chr8:44223914-44223933 MsG0880044550.01.T01:intron 10.0%
!!! TCAAAATATATTCATTTTTT+GGG + Chr8:44222839-44222858 None:intergenic 10.0%
!!! TCTAAAATAATGAATTTTAA+AGG + Chr8:44223170-44223189 None:intergenic 10.0%
!!! TTCAAAATATATTCATTTTT+TGG + Chr8:44222840-44222859 None:intergenic 10.0%
!! AAAACTAATTATAAATGAGA+AGG + Chr8:44225178-44225197 None:intergenic 15.0%
!! AAGAAATAAAGTTTCTTTAA+TGG - Chr8:44225034-44225053 MsG0880044550.01.T01:intron 15.0%
!! AAGAGAGAAATAAAAAAAAA+AGG - Chr8:44224834-44224853 MsG0880044550.01.T01:intron 15.0%
!! AATAAAATTGGAAGAAAAAA+TGG + Chr8:44224630-44224649 None:intergenic 15.0%
!! AGAAATAAAGTTTCTTTAAT+GGG - Chr8:44225035-44225054 MsG0880044550.01.T01:intron 15.0%
!! AGAAATTGAAGTTATTAATT+AGG + Chr8:44224222-44224241 None:intergenic 15.0%
!! GAAATTGAAGTTATTAATTA+GGG + Chr8:44224221-44224240 None:intergenic 15.0%
!! GTAAAAATAGAGAATAAAAT+TGG + Chr8:44224642-44224661 None:intergenic 15.0%
!! GTTTGTATTATTATTGAAAA+TGG + Chr8:44222068-44222087 None:intergenic 15.0%
!! TAAAGAATCTTAAACATTTA+TGG + Chr8:44223907-44223926 None:intergenic 15.0%
!!! ATCTCTTTTTAATGTTAAAT+TGG + Chr8:44225279-44225298 None:intergenic 15.0%
!!! CAAAATATATTCATTTTTTG+GGG + Chr8:44222838-44222857 None:intergenic 15.0%
!!! CTCATTTATAATTAGTTTTT+TGG - Chr8:44225179-44225198 MsG0880044550.01.T01:intron 15.0%
!!! GATTATTTAAGTTGTTTAAT+AGG - Chr8:44223846-44223865 MsG0880044550.01.T01:intron 15.0%
!! AATAAAGAACATACATAAAC+GGG + Chr8:44221959-44221978 None:intergenic 20.0%
!! AATAATACAAACATTTGAGA+AGG - Chr8:44222076-44222095 MsG0880044550.01.T01:intron 20.0%
!! ATAATCATAGTAGTTAATAG+CGG + Chr8:44224505-44224524 None:intergenic 20.0%
!! ATGATCAATTTCTAATCTTA+AGG + Chr8:44223369-44223388 None:intergenic 20.0%
!! CAAAAAGTTATATGAAATAG+CGG - Chr8:44224418-44224437 MsG0880044550.01.T01:intron 20.0%
!! CAATAAAGAACATACATAAA+CGG + Chr8:44221960-44221979 None:intergenic 20.0%
!! GAAAATCAAGAAACAAATAA+TGG - Chr8:44223295-44223314 MsG0880044550.01.T01:intron 20.0%
!! GTTTGTATAAATATGTCTAT+TGG + Chr8:44222706-44222725 None:intergenic 20.0%
!! TAAAAATCTATTATGTACTC+AGG - Chr8:44225374-44225393 MsG0880044550.01.T01:intron 20.0%
!! TAAGATTAGAAATTGATCAT+AGG - Chr8:44223369-44223388 MsG0880044550.01.T01:CDS 20.0%
!! TATGGAATTGAAATTACTAT+GGG + Chr8:44223889-44223908 None:intergenic 20.0%
!! TTATGGAATTGAAATTACTA+TGG + Chr8:44223890-44223909 None:intergenic 20.0%
!! TTATTATTGAAAATGGAAAC+AGG + Chr8:44222061-44222080 None:intergenic 20.0%
!!! ACTTTATTTCTTCATCTTTT+TGG + Chr8:44225026-44225045 None:intergenic 20.0%
!!! CATTTATGTGTCATTTTTAT+CGG - Chr8:44222487-44222506 MsG0880044550.01.T01:intron 20.0%
!!! TCAATTTTTACTTTGTATGT+GGG + Chr8:44222200-44222219 None:intergenic 20.0%
!!! TGGTTTGAAATAAAAAACAA+GGG - Chr8:44224182-44224201 MsG0880044550.01.T01:intron 20.0%
!!! TTCAATTTTTACTTTGTATG+TGG + Chr8:44222201-44222220 None:intergenic 20.0%
!!! TTGATTTTCCTACTTATTTA+GGG + Chr8:44223284-44223303 None:intergenic 20.0%
!!! TTGGTTTGAAATAAAAAACA+AGG - Chr8:44224181-44224200 MsG0880044550.01.T01:intron 20.0%
!!! TTGTTTTTTATTTCAAACCA+AGG + Chr8:44224182-44224201 None:intergenic 20.0%
! ATGAAAACTGCAAATTAGTT+AGG + Chr8:44224880-44224899 None:intergenic 25.0%
! ATGCAAGAGACTAAAAATAA+TGG - Chr8:44222656-44222675 MsG0880044550.01.T01:intron 25.0%
! GAAAACTGGAAATTAGTTAT+GGG - Chr8:44224999-44225018 MsG0880044550.01.T01:intron 25.0%
! GATTATGAGTTGTTTACTTA+GGG - Chr8:44224519-44224538 MsG0880044550.01.T01:intron 25.0%
! TAATGGAATCTGACTTTATA+GGG - Chr8:44222673-44222692 MsG0880044550.01.T01:intron 25.0%
! TCAACTCACAGAAAAATTAT+AGG + Chr8:44222784-44222803 None:intergenic 25.0%
! TGAAAACTGCAAATTAGTTA+GGG + Chr8:44224879-44224898 None:intergenic 25.0%
! TGAAAACTGGAAATTAGTTA+TGG - Chr8:44224998-44225017 MsG0880044550.01.T01:intron 25.0%
! TGATTATGAGTTGTTTACTT+AGG - Chr8:44224518-44224537 MsG0880044550.01.T01:intron 25.0%
! TGGATAATAACTAAGCAATA+TGG - Chr8:44223315-44223334 MsG0880044550.01.T01:intron 25.0%
! TGTTAAATAACTGTATGTAC+TGG - Chr8:44222322-44222341 MsG0880044550.01.T01:intron 25.0%
! TTCATCAGTGAAAACTTAAA+AGG + Chr8:44223586-44223605 None:intergenic 25.0%
! TTTAATGTTAAATTGGCATG+AGG + Chr8:44225272-44225291 None:intergenic 25.0%
! TTTCACGTAAAATGAAATAG+CGG + Chr8:44224147-44224166 None:intergenic 25.0%
!! ATAATGGAATCTGACTTTAT+AGG - Chr8:44222672-44222691 MsG0880044550.01.T01:intron 25.0%
!! CTAATTTCCAGTTTTCATTT+TGG + Chr8:44224995-44225014 None:intergenic 25.0%
!! CTGTGATTTTTAATCTATTG+TGG - Chr8:44223700-44223719 MsG0880044550.01.T01:intron 25.0%
!! CTTGATTTTCCTACTTATTT+AGG + Chr8:44223285-44223304 None:intergenic 25.0%
!! TGATTGATTCTTTGTTCATT+GGG - Chr8:44225124-44225143 MsG0880044550.01.T01:intron 25.0%
!! TTGATTGATTCTTTGTTCAT+TGG - Chr8:44225123-44225142 MsG0880044550.01.T01:intron 25.0%
!!! AGTGTTGATTTTTTGAGTTA+TGG + Chr8:44224910-44224929 None:intergenic 25.0%
!!! ATATATTCATTTTTTGGGGT+CGG + Chr8:44222834-44222853 None:intergenic 25.0%
!!! CGCTATTTCATATAACTTTT+TGG + Chr8:44224420-44224439 None:intergenic 25.0%
!!! CTAATTTGCAGTTTTCATTT+TGG - Chr8:44224882-44224901 MsG0880044550.01.T01:intron 25.0%
!!! GTGTTGATTTTTTGAGTTAT+GGG + Chr8:44224909-44224928 None:intergenic 25.0%
!!! TTTAACTCCAAGTTTTATCA+AGG - Chr8:44225059-44225078 MsG0880044550.01.T01:intron 25.0%
!!! TTTAGAGAAGATGCTTTTTA+GGG + Chr8:44224349-44224368 None:intergenic 25.0%
!!! TTTTAGAGAAGATGCTTTTT+AGG + Chr8:44224350-44224369 None:intergenic 25.0%
AAAAACGATGATATGGCAAA+CGG + Chr8:44222617-44222636 None:intergenic 30.0%
AAAAGGCTTAATTAGTCCAA+TGG - Chr8:44224851-44224870 MsG0880044550.01.T01:intron 30.0%
AAATGACACATAAATGCAAG+TGG + Chr8:44222483-44222502 None:intergenic 30.0%
AACACTAACCCTAAATAAGT+AGG - Chr8:44223273-44223292 MsG0880044550.01.T01:intron 30.0%
AAGAAGAACATCAACAATGT+TGG - Chr8:44224764-44224783 MsG0880044550.01.T01:intron 30.0%
AAGTGTTCCTTGATAAAACT+TGG + Chr8:44225069-44225088 None:intergenic 30.0%
ACAAAGAATCAATCAATCTG+TGG + Chr8:44225119-44225138 None:intergenic 30.0%
ACGTTTGTAATCTATACCAA+TGG - Chr8:44224585-44224604 MsG0880044550.01.T01:intron 30.0%
AGAGAAGATTTAATGAAGAG+AGG + Chr8:44224307-44224326 None:intergenic 30.0%
ATCAGATAATATGACACATG+TGG - Chr8:44222587-44222606 MsG0880044550.01.T01:intron 30.0%
ATGACGTCAATAATGACAAT+AGG + Chr8:44223671-44223690 None:intergenic 30.0%
ATGAGCATAGTAGTTAATAG+CGG - Chr8:44223988-44224007 MsG0880044550.01.T01:intron 30.0%
ATGTGTCATATTATCTGATG+TGG + Chr8:44222586-44222605 None:intergenic 30.0%
CGTTATAATCAGTCAATTGA+TGG + Chr8:44222527-44222546 None:intergenic 30.0%
CTCTCTTCTTTCATTCTTTA+CGG - Chr8:44224240-44224259 MsG0880044550.01.T01:intron 30.0%
GAAAACTGCAAATTAGTTAG+GGG + Chr8:44224878-44224897 None:intergenic 30.0%
GCATTACAAAAACGATGATA+TGG + Chr8:44222624-44222643 None:intergenic 30.0%
TAATCAGTCAATTGATGGAA+TGG + Chr8:44222522-44222541 None:intergenic 30.0%
TACTTAGGGTTATCATAAGT+AGG - Chr8:44224533-44224552 MsG0880044550.01.T01:intron 30.0%
TGAAAATACAAGATTCTCCT+TGG - Chr8:44224162-44224181 MsG0880044550.01.T01:intron 30.0%
! AAACAATTTACCCTCTAAAG+TGG + Chr8:44223958-44223977 None:intergenic 30.0%
! ACAAAATCTGACTTGCAAAA+TGG + Chr8:44225531-44225550 None:intergenic 30.0%
! ATTCTTTGTTCATTGGGTTT+TGG - Chr8:44225130-44225149 MsG0880044550.01.T01:intron 30.0%
! ATTTTTACTTTGTATGTGGG+TGG + Chr8:44222197-44222216 None:intergenic 30.0%
! CAAAATCTGACTTGCAAAAT+GGG + Chr8:44225530-44225549 None:intergenic 30.0%
! CACTTTTACCAAAAGAAAGT+AGG - Chr8:44225084-44225103 MsG0880044550.01.T01:intron 30.0%
! CTTGTTATGAGTATTTGTGT+TGG - Chr8:44221757-44221776 MsG0880044550.01.T01:intron 30.0%
! TGTATTCTTTTATGCTGACA+GGG - Chr8:44223488-44223507 MsG0880044550.01.T01:intron 30.0%
! TTGTATTCTTTTATGCTGAC+AGG - Chr8:44223487-44223506 MsG0880044550.01.T01:intron 30.0%
! TTTTGCTCCGTTATTCAATT+TGG - Chr8:44224951-44224970 MsG0880044550.01.T01:intron 30.0%
!! AACAATTTACCCTCTAAAGT+GGG + Chr8:44223957-44223976 None:intergenic 30.0%
!! AAGTTCTAAGTGGTAAATAC+TGG + Chr8:44224742-44224761 None:intergenic 30.0%
!! TTCTTCTTTCAAGTTCTAAG+TGG + Chr8:44224752-44224771 None:intergenic 30.0%
AAATATTACCTGAACCGTCT+CGG + Chr8:44225481-44225500 None:intergenic 35.0%
AAATGAGAAGGATGCGAAAT+TGG + Chr8:44225166-44225185 None:intergenic 35.0%
AACAAAGTAACTGCAGTAAC+AGG + Chr8:44222386-44222405 None:intergenic 35.0%
ACTTTCACTTTCTCAAACAG+AGG - Chr8:44222456-44222475 MsG0880044550.01.T01:intron 35.0%
AGAACATCAACAATGTTGGA+TGG - Chr8:44224768-44224787 MsG0880044550.01.T01:intron 35.0%
AGAGATGTGTAACACTTGTA+TGG + Chr8:44221824-44221843 None:intergenic 35.0%
GAATCTCAGATGCTAAGTTA+TGG - Chr8:44222298-44222317 MsG0880044550.01.T01:intron 35.0%
GGCATTAGTGTTTCTTGAAA+TGG - Chr8:44222097-44222116 MsG0880044550.01.T01:intron 35.0%
TCTCTCTATCTCAGTTGAAA+AGG - Chr8:44222144-44222163 MsG0880044550.01.T01:intron 35.0%
TTCAACAGAGTATGCTTTCA+AGG - Chr8:44221615-44221634 MsG0880044550.01.T01:CDS 35.0%
TTGTGTGGTTACTCAGAAAA+AGG - Chr8:44221690-44221709 MsG0880044550.01.T01:CDS 35.0%
TTTCCTATTACCAAGTACCT+TGG - Chr8:44221843-44221862 MsG0880044550.01.T01:intron 35.0%
! AAACCAAGGTACTTGGTAAT+AGG + Chr8:44221849-44221868 None:intergenic 35.0%
! AACGAAGACCTACTTTCTTT+TGG + Chr8:44225095-44225114 None:intergenic 35.0%
! AAGTATGCAACTTTTGCACA+AGG + Chr8:44223618-44223637 None:intergenic 35.0%
! AATAACGGAGCAAAAGTTGA+GGG + Chr8:44224946-44224965 None:intergenic 35.0%
! ACTTCACAGATCTAGAACAA+AGG + Chr8:44221916-44221935 None:intergenic 35.0%
! AGACGGTTCAGGTAATATTT+TGG - Chr8:44225481-44225500 MsG0880044550.01.T01:CDS 35.0%
! TACCAATGGATACTGAGTTT+TGG - Chr8:44224599-44224618 MsG0880044550.01.T01:intron 35.0%
! TATCCCGCTATAGCATTTTT+GGG - Chr8:44224457-44224476 MsG0880044550.01.T01:intron 35.0%
!! AGTATTTGTGTTGGCTGATT+AGG - Chr8:44221766-44221785 MsG0880044550.01.T01:intron 35.0%
!! TAGTAGTTAATAGCGGATAG+CGG + Chr8:44224498-44224517 None:intergenic 35.0%
!!! TGTGTCATTTTTATCGGATG+TGG - Chr8:44222493-44222512 MsG0880044550.01.T01:intron 35.0%
!!! TTAGTTTTTTGGAGTGTTGC+AGG - Chr8:44225190-44225209 MsG0880044550.01.T01:intron 35.0%
AAAATGAGCTCCCACTTTAG+AGG - Chr8:44223944-44223963 MsG0880044550.01.T01:intron 40.0%
AAACACATACCTACTCCAAG+TGG + Chr8:44223558-44223577 None:intergenic 40.0%
AAATGAGCTCCCACTTTAGA+GGG - Chr8:44223945-44223964 MsG0880044550.01.T01:intron 40.0%
AATTTGGTCCCCTCAAGTTA+CGG - Chr8:44224967-44224986 MsG0880044550.01.T01:intron 40.0%
AGCAAAAGTTGAGGGATCAT+TGG + Chr8:44224938-44224957 None:intergenic 40.0%
AGGGTCACATTTGTAAAGCT+GGG + Chr8:44223769-44223788 None:intergenic 40.0%
AGTACAGGAAGTTGACTGTT+AGG - Chr8:44221721-44221740 MsG0880044550.01.T01:CDS 40.0%
AGTGAAGTTGCTATGGTTCT+GGG - Chr8:44223723-44223742 MsG0880044550.01.T01:intron 40.0%
ATTGTGGAGTGAAGTTGCTA+TGG - Chr8:44223716-44223735 MsG0880044550.01.T01:intron 40.0%
CAAATTAGTTAGGGGACCAT+TGG + Chr8:44224870-44224889 None:intergenic 40.0%
CACCAAAACTCAGTATCCAT+TGG + Chr8:44224604-44224623 None:intergenic 40.0%
CAGATGCGTACAAATCTGAA+AGG + Chr8:44224268-44224287 None:intergenic 40.0%
CAGGTGATAGGTAATGTTTG+GGG - Chr8:44225209-44225228 MsG0880044550.01.T01:intron 40.0%
CATGCTTATATGAGACCACT+TGG - Chr8:44223540-44223559 MsG0880044550.01.T01:intron 40.0%
CCAAGAAATTGATTGCCTCT+TGG + Chr8:44225422-44225441 None:intergenic 40.0%
CCAAGAGGCAATCAATTTCT+TGG - Chr8:44225419-44225438 MsG0880044550.01.T01:intron 40.0%
GAGGGGACCAAATTGAATAA+CGG + Chr8:44224961-44224980 None:intergenic 40.0%
GCAGGTGATAGGTAATGTTT+GGG - Chr8:44225208-44225227 MsG0880044550.01.T01:intron 40.0%
GGGAAAAGATTCAGTTTGTG+TGG - Chr8:44221675-44221694 MsG0880044550.01.T01:CDS 40.0%
GGTTACTCAGAAAAAGGTTC+CGG - Chr8:44221696-44221715 MsG0880044550.01.T01:CDS 40.0%
TACCAAGTACCTTGGTTTAC+TGG - Chr8:44221851-44221870 MsG0880044550.01.T01:intron 40.0%
TACGGGACCAAAATGAAAAC+TGG - Chr8:44224985-44225004 MsG0880044550.01.T01:intron 40.0%
TAGGTGGGATTGTTTAATGC+AGG - Chr8:44221785-44221804 MsG0880044550.01.T01:intron 40.0%
TATATGAGACCACTTGGAGT+AGG - Chr8:44223546-44223565 MsG0880044550.01.T01:intron 40.0%
TCTCGGCATAGGTAAATGAA+GGG + Chr8:44225464-44225483 None:intergenic 40.0%
TGCAGGTGATAGGTAATGTT+TGG - Chr8:44225207-44225226 MsG0880044550.01.T01:intron 40.0%
TGGAATCTGACTTTATAGGG+AGG - Chr8:44222676-44222695 MsG0880044550.01.T01:intron 40.0%
TGGTCATTACTTTGGTCACA+AGG - Chr8:44223791-44223810 MsG0880044550.01.T01:intron 40.0%
TGTACTGGAATATGCTGAGT+CGG - Chr8:44222337-44222356 MsG0880044550.01.T01:intron 40.0%
TTACTTTGTATGTGGGTGGT+TGG + Chr8:44222193-44222212 None:intergenic 40.0%
TTCATTTACCTATGCCGAGA+CGG - Chr8:44225464-44225483 MsG0880044550.01.T01:CDS 40.0%
! ATCCCGCTATAGCATTTTTG+GGG - Chr8:44224458-44224477 MsG0880044550.01.T01:intron 40.0%
! CTATCCCGCTATAGCATTTT+TGG - Chr8:44224456-44224475 MsG0880044550.01.T01:intron 40.0%
! GAATAACGGAGCAAAAGTTG+AGG + Chr8:44224947-44224966 None:intergenic 40.0%
! TGAGGCATCATTGTAATGCA+AGG + Chr8:44225254-44225273 None:intergenic 40.0%
!! ATAACCTCGATTGGTGTTAG+GGG - Chr8:44221655-44221674 MsG0880044550.01.T01:CDS 40.0%
!! ATTTGTGTTGGCTGATTAGG+TGG - Chr8:44221769-44221788 MsG0880044550.01.T01:intron 40.0%
!! ATTTTGGTCCCGTAACTTGA+GGG + Chr8:44224979-44224998 None:intergenic 40.0%
!! GATAACCTCGATTGGTGTTA+GGG - Chr8:44221654-44221673 MsG0880044550.01.T01:CDS 40.0%
!! TGGGTATTGACGATGAACTT+GGG - Chr8:44223742-44223761 MsG0880044550.01.T01:intron 40.0%
!! TTTGTGTTGGCTGATTAGGT+GGG - Chr8:44221770-44221789 MsG0880044550.01.T01:intron 40.0%
AAAAAGGTTCCGGTGAGTAC+AGG - Chr8:44221706-44221725 MsG0880044550.01.T01:CDS 45.0%
AAAACCGCTATGCAGTGTTG+CGG + Chr8:44224113-44224132 None:intergenic 45.0%
AACGATGATATGGCAAACGG+AGG + Chr8:44222614-44222633 None:intergenic 45.0%
AGTCAACTTCCTGTACTCAC+CGG + Chr8:44221718-44221737 None:intergenic 45.0%
AGTTAATAGCGGCGCTATAG+CGG - Chr8:44223999-44224018 MsG0880044550.01.T01:intron 45.0%
ATCTGGATATGAGATGCCTG+TGG - Chr8:44223045-44223064 MsG0880044550.01.T01:intron 45.0%
ATTTGGTCCCCTCAAGTTAC+GGG - Chr8:44224968-44224987 MsG0880044550.01.T01:intron 45.0%
CAGGGTCACATTTGTAAAGC+TGG + Chr8:44223770-44223789 None:intergenic 45.0%
CATTTAGACAGCACATCCAC+AGG + Chr8:44223064-44223083 None:intergenic 45.0%
CGACCCCAAAAATGCTATAG+CGG + Chr8:44224464-44224483 None:intergenic 45.0%
CTGTGGATGTGCTGTCTAAA+TGG - Chr8:44223062-44223081 MsG0880044550.01.T01:intron 45.0%
CTTTACAAATGTGACCCTGC+TGG - Chr8:44223771-44223790 MsG0880044550.01.T01:intron 45.0%
GACCAAAGTAATGACCAGCA+GGG + Chr8:44223788-44223807 None:intergenic 45.0%
GACCCCAAAAATGCTATAGC+GGG + Chr8:44224463-44224482 None:intergenic 45.0%
GAGAAGGATGCGAAATTGGA+AGG + Chr8:44225162-44225181 None:intergenic 45.0%
GAGTGAAGTTGCTATGGTTC+TGG - Chr8:44223722-44223741 MsG0880044550.01.T01:intron 45.0%
GCAAGGAATCCCAAACATGT+TGG + Chr8:44225237-44225256 None:intergenic 45.0%
GGATGTGCTGTCTAAATGGT+AGG - Chr8:44223066-44223085 MsG0880044550.01.T01:intron 45.0%
GGGTCACATTTGTAAAGCTG+GGG + Chr8:44223768-44223787 None:intergenic 45.0%
GTCCTTTAATCCAGCACTTG+TGG + Chr8:44225400-44225419 None:intergenic 45.0%
GTCTCGGCATAGGTAAATGA+AGG + Chr8:44225465-44225484 None:intergenic 45.0%
TATCTCATAACGCTACGCCT+TGG + Chr8:44224075-44224094 None:intergenic 45.0%
TCAAGGCTGTTAAGTCTGCA+GGG - Chr8:44221632-44221651 MsG0880044550.01.T01:CDS 45.0%
TGACCAAAGTAATGACCAGC+AGG + Chr8:44223789-44223808 None:intergenic 45.0%
TGCTGGATTAAAGGACCAAG+AGG - Chr8:44225404-44225423 MsG0880044550.01.T01:intron 45.0%
TGGGTGGTTGGAATGTCATT+TGG + Chr8:44222181-44222200 None:intergenic 45.0%
TTCAAGGCTGTTAAGTCTGC+AGG - Chr8:44221631-44221650 MsG0880044550.01.T01:CDS 45.0%
TTTCTGACTGCAACCGATCA+AGG + Chr8:44225336-44225355 None:intergenic 45.0%
! ACTTCTTTTCAGCTGATGCC+AGG - Chr8:44222975-44222994 MsG0880044550.01.T01:intron 45.0%
! GCTGAGTATCGCTTTAGATC+TGG - Chr8:44223028-44223047 MsG0880044550.01.T01:intron 45.0%
! GGATAACCTCGATTGGTGTT+AGG - Chr8:44221653-44221672 MsG0880044550.01.T01:CDS 45.0%
! TGACTTGCAAAATGGGAGTG+AGG + Chr8:44225523-44225542 None:intergenic 45.0%
! TGCTTGTTGAACTAGTGTCC+TGG + Chr8:44222996-44223015 None:intergenic 45.0%
! TTTGGGGTGCCAACATGTTT+GGG - Chr8:44225225-44225244 MsG0880044550.01.T01:intron 45.0%
! TTTTCCCCTAACACCAATCG+AGG + Chr8:44221662-44221681 None:intergenic 45.0%
! TTTTGGTCCCGTAACTTGAG+GGG + Chr8:44224978-44224997 None:intergenic 45.0%
!! CATTTTGGTCCCGTAACTTG+AGG + Chr8:44224980-44224999 None:intergenic 45.0%
!! CTGGGTATTGACGATGAACT+TGG - Chr8:44223741-44223760 MsG0880044550.01.T01:intron 45.0%
!! TTTGGAGTGTTGCAGGTGAT+AGG - Chr8:44225197-44225216 MsG0880044550.01.T01:intron 45.0%
!!! CGCTATAGCATTTTTGGGGT+CGG - Chr8:44224462-44224481 MsG0880044550.01.T01:intron 45.0%
!! AATTAAATTTAATATGAAAA+TGG - Chr8:44222557-44222576 MsG0880044550.01.T01:intron 5.0%
!!! TTTTCAAAAAAAAAAAAAAA+TGG + Chr8:44224709-44224728 None:intergenic 5.0%
CGCCTTGGCTTATTGCATAG+CGG + Chr8:44224060-44224079 None:intergenic 50.0%
GAAATAGCGGTCATAGCGCT+AGG - Chr8:44224431-44224450 MsG0880044550.01.T01:intron 50.0%
GAACATACCTGTGATGCCAG+TGG + Chr8:44221877-44221896 None:intergenic 50.0%
GACCCTGCTGGTCATTACTT+TGG - Chr8:44223783-44223802 MsG0880044550.01.T01:intron 50.0%
GACCGCTATGCAATAAGCCA+AGG - Chr8:44224055-44224074 MsG0880044550.01.T01:intron 50.0%
GGCGTAGCGTTATGAGATAG+CGG - Chr8:44224076-44224095 MsG0880044550.01.T01:intron 50.0%
TACCTTGGTTTACTGGCCAC+TGG - Chr8:44221858-44221877 MsG0880044550.01.T01:intron 50.0%
TCTGCAGGGATAACCTCGAT+TGG - Chr8:44221646-44221665 MsG0880044550.01.T01:CDS 50.0%
! ATGAGATAGCGGCGCAATAG+CGG - Chr8:44224087-44224106 MsG0880044550.01.T01:CDS 50.0%
! GGCCAGTAAACCAAGGTACT+TGG + Chr8:44221856-44221875 None:intergenic 50.0%
! GTTTGGGGTGCCAACATGTT+TGG - Chr8:44225224-44225243 MsG0880044550.01.T01:intron 50.0%
!! GGCCACAAGTGCTGGATTAA+AGG - Chr8:44225395-44225414 MsG0880044550.01.T01:intron 50.0%
GGCTTATTGCATAGCGGTCC+TGG + Chr8:44224054-44224073 None:intergenic 55.0%
TACCTATGCCGAGACGGTTC+AGG - Chr8:44225470-44225489 MsG0880044550.01.T01:CDS 55.0%
TACCTGAACCGTCTCGGCAT+AGG + Chr8:44225475-44225494 None:intergenic 55.0%
TGCCAGTGGCCAGTAAACCA+AGG + Chr8:44221863-44221882 None:intergenic 55.0%
TGTACTCAGGCCACAAGTGC+TGG - Chr8:44225387-44225406 MsG0880044550.01.T01:intron 55.0%
TTACTGGCCACTGGCATCAC+AGG - Chr8:44221867-44221886 MsG0880044550.01.T01:intron 55.0%
CAACCGATCAAGGACGCGTG+AGG + Chr8:44225326-44225345 None:intergenic 60.0%
GCACCTCACGCGTCCTTGAT+CGG - Chr8:44225320-44225339 MsG0880044550.01.T01:CDS 60.0%
GGCGCTATAGCGGCCAATAG+CGG - Chr8:44224009-44224028 MsG0880044550.01.T01:intron 60.0%
GCGGCCGCAACACTGCATAG+CGG - Chr8:44224106-44224125 MsG0880044550.01.T01:CDS 65.0%
! TAGCGGATAGCGGCGCGTAG+CGG + Chr8:44224488-44224507 None:intergenic 65.0%
AGCGGCCAATAGCGGCCGCG+CGG - Chr8:44224017-44224036 MsG0880044550.01.T01:intron 75.0%
CCAATAGCGGCCGCGCGGTG+CGG - Chr8:44224022-44224041 MsG0880044550.01.T01:intron 75.0%
! CCGCACCGCGCGGCCGCTAT+TGG + Chr8:44224025-44224044 None:intergenic 80.0%
! CTGGACCGCACCGCACCGCG+CGG + Chr8:44224035-44224054 None:intergenic 80.0%
! AGCGGCCGCGCGGTGCGGTG+CGG - Chr8:44224027-44224046 MsG0880044550.01.T01:intron 85.0%
! CGCGCGGTGCGGTGCGGTCC+AGG - Chr8:44224033-44224052 MsG0880044550.01.T01:intron 85.0%
Chromosome Type Strat End Strand Name
Chr8 gene 44221610 44225554 44221610 ID=MsG0880044550.01;Name=MsG0880044550.01
Chr8 mRNA 44221610 44225554 44221610 ID=MsG0880044550.01.T01;Parent=MsG0880044550.01;Name=MsG0880044550.01.T01;_AED=0.49;_eAED=0.59;_QI=0|0.6|0.5|1|0.8|0.66|6|0|211
Chr8 exon 44225447 44225554 44225447 ID=MsG0880044550.01.T01:exon:17811;Parent=MsG0880044550.01.T01
Chr8 exon 44225247 44225376 44225247 ID=MsG0880044550.01.T01:exon:17810;Parent=MsG0880044550.01.T01
Chr8 exon 44224081 44224177 44224081 ID=MsG0880044550.01.T01:exon:17809;Parent=MsG0880044550.01.T01
Chr8 exon 44223597 44223655 44223597 ID=MsG0880044550.01.T01:exon:17808;Parent=MsG0880044550.01.T01
Chr8 exon 44223352 44223434 44223352 ID=MsG0880044550.01.T01:exon:17807;Parent=MsG0880044550.01.T01
Chr8 exon 44221610 44221768 44221610 ID=MsG0880044550.01.T01:exon:17806;Parent=MsG0880044550.01.T01
Chr8 CDS 44225447 44225554 44225447 ID=MsG0880044550.01.T01:cds;Parent=MsG0880044550.01.T01
Chr8 CDS 44225247 44225376 44225247 ID=MsG0880044550.01.T01:cds;Parent=MsG0880044550.01.T01
Chr8 CDS 44224081 44224177 44224081 ID=MsG0880044550.01.T01:cds;Parent=MsG0880044550.01.T01
Chr8 CDS 44223597 44223655 44223597 ID=MsG0880044550.01.T01:cds;Parent=MsG0880044550.01.T01
Chr8 CDS 44223352 44223434 44223352 ID=MsG0880044550.01.T01:cds;Parent=MsG0880044550.01.T01
Chr8 CDS 44221610 44221768 44221610 ID=MsG0880044550.01.T01:cds;Parent=MsG0880044550.01.T01
Gene Sequence

>MsG0880044550.01.T01

ATGTGTTCAACAGAGTATGCTTTCAAGGCTGTTAAGTCTGCAGGGATAACCTCGATTGGTGTTAGGGGAAAAGATTCAGTTTGTGTGGTTACTCAGAAAAAGGTTCCGGTGGGATTGTTTAATGCAGGACAAGCTCTTAGACCATACAAGTGTTACACATCTCTTTCCTATTACCAAGTACCTTGGTTTACTGGCCACTGGCATCACAGGTATGTTCTTCATTACAACTATTTCCTTTCTGATGCCAGGACACTAGTTCAACAAGCAAGAAATGAAGCTGCTGAGTATCGCTTTAGATCTGGATATGAGATGCCTGTGGATGTGCTGTCTAAATGGGTTGCAGACAAATCACAAGTCTATACTCAACATGCTTATATGAGACCACTTGGAGTAGTTGCTATGGTTCTGGGTATTGACGATGAACTTGGGCCCCAGCTTTACAAATGTGACCCTGCTGGTCATTACTTTGGTCACAAGGCCACAAGTGCTGGATTAAAGGACCAAGAGGCAATCAATTTCTTGGAAAAGAAGATGAAGAATGACCCTTCATTTACCTATGCCGAGACGGTTCAGGTAATATTTTGGCTTTGTTCAATTTTTTCCTCACTCCCATTTTGCAAGTCAGATTTTGTGTGA
Protein sequence

>MsG0880044550.01.T01

MCSTEYAFKAVKSAGITSIGVRGKDSVCVVTQKKVPVGLFNAGQALRPYKCYTSLSYYQVPWFTGHWHHRYVLHYNYFLSDARTLVQQARNEAAEYRFRSGYEMPVDVLSKWVADKSQVYTQHAYMRPLGVVAMVLGIDDELGPQLYKCDPAGHYFGHKATSAGLKDQEAINFLEKKMKNDPSFTYAETVQVIFWLCSIFSSLPFCKSDFV*