AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar ZhongmuNo.1 / MsG0180001547.01

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsG0180001547.01.T01 MTR_4g012740 73.025 519 50 6 10 443 37 550 0.0 689
MsG0180001547.01.T01 MTR_4g012740 62.679 209 32 5 51 246 1 176 1.61e-71 235
MsG0180001547.01.T01 MTR_1g022410 75.988 329 27 5 122 443 1 284 1.07e-150 429
MsG0180001547.01.T01 MTR_1g022410 81.159 69 7 1 51 119 1 63 1.38e-26 108
MsG0180001547.01.T01 MTR_1g022410 63.492 63 8 1 1 48 1 63 2.49e-13 70.5
MsG0180001547.01.T01 MTR_4g057090 60.584 411 84 9 38 443 4 341 1.03e-144 416
MsG0180001547.01.T01 MTR_4g057090 62.385 109 36 3 11 119 43 146 7.07e-29 116
MsG0180001547.01.T01 MTR_3g113730 85.366 164 21 2 281 443 4 165 6.49e-95 283
MsG0180001547.01.T01 MTR_1g112250 38.418 177 89 5 172 339 19 184 2.10e-24 101
MsG0180001547.01.T01 MTR_7g070070 34.597 211 112 8 172 369 16 213 4.00e-23 98.2
MsG0180001547.01.T01 MTR_5g016590 33.790 219 119 8 172 377 16 221 9.69e-23 97.1
MsG0180001547.01.T01 MTR_0167s0060 30.000 230 141 7 162 385 7 222 1.68e-22 96.3
MsG0180001547.01.T01 MTR_8g464460 29.694 229 142 8 172 392 20 237 3.78e-20 89.7
MsG0180001547.01.T01 MTR_8g075320 31.754 211 120 8 172 372 15 211 1.03e-19 88.6
MsG0180001547.01.T01 MTR_2g060830 28.821 229 144 8 172 392 20 237 6.21e-19 86.3
MsG0180001547.01.T01 MTR_2g016050 32.367 207 124 7 172 372 15 211 7.36e-19 86.3
MsG0180001547.01.T01 MTR_6g024135 30.366 191 113 7 172 355 19 196 2.60e-16 79.0
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsG0180001547.01.T01 AT1G47250 61.043 326 74 6 122 443 1 277 4.88e-125 364
MsG0180001547.01.T01 AT1G47250 73.913 69 12 1 51 119 1 63 5.28e-24 101
MsG0180001547.01.T01 AT1G47250 57.143 63 12 1 1 48 1 63 2.09e-11 64.7
MsG0180001547.01.T01 AT5G42790 65.018 283 50 4 122 400 1 238 2.30e-124 362
MsG0180001547.01.T01 AT5G42790 73.913 69 12 1 51 119 1 63 6.05e-24 101
MsG0180001547.01.T01 AT5G42790 57.143 63 12 1 1 48 1 63 2.21e-11 64.7
MsG0180001547.01.T01 AT1G53850 37.853 177 90 5 172 339 19 184 5.99e-24 100
MsG0180001547.01.T01 AT1G53850 37.853 177 90 5 172 339 19 184 5.99e-24 100
MsG0180001547.01.T01 AT3G14290 37.853 177 90 5 172 339 19 184 6.49e-24 100
MsG0180001547.01.T01 AT3G22110 33.173 208 119 7 172 369 16 213 2.93e-23 98.6
MsG0180001547.01.T01 AT1G16470 30.000 230 141 8 162 385 7 222 1.97e-21 93.2
MsG0180001547.01.T01 AT1G16470 30.000 230 141 8 162 385 7 222 1.97e-21 93.2
MsG0180001547.01.T01 AT1G79210 29.565 230 142 8 162 385 7 222 5.84e-21 91.7
MsG0180001547.01.T01 AT1G79210 29.565 230 142 8 162 385 7 222 5.84e-21 91.7
MsG0180001547.01.T01 AT1G79210 29.565 230 142 8 162 385 7 222 5.84e-21 91.7
MsG0180001547.01.T01 AT5G66140 31.754 211 120 7 172 372 15 211 1.16e-19 88.6
MsG0180001547.01.T01 AT3G51260 30.806 211 122 7 172 372 15 211 5.44e-19 86.7
MsG0180001547.01.T01 AT3G51260 30.806 211 122 7 172 372 15 211 8.15e-19 85.9
MsG0180001547.01.T01 AT5G35590 29.258 229 143 8 172 392 20 237 1.34e-18 85.5
MsG0180001547.01.T01 AT2G05840 28.384 229 145 8 172 392 20 237 3.46e-17 81.3
MsG0180001547.01.T01 AT2G05840 29.293 198 123 6 172 362 20 207 5.90e-16 77.0
MsG0180001547.01.T01 AT2G05840 28.877 187 116 6 172 351 20 196 4.43e-14 71.2
MsG0180001547.01.T01 AT2G27020 28.723 188 120 5 172 355 19 196 1.08e-13 71.2
MsG0180001547.01.T01 AT2G27020 28.723 188 120 5 172 355 121 298 5.13e-13 70.5

Find 132 sgRNAs with CRISPR-Local

Find 130 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
ACCACCTTGGTCTTGGTTTC+GGG 0.158116 1:+23055297 None:intergenic
CGCGAGAACCACGTGAGTTT+TGG 0.220139 1:+23055972 None:intergenic
CAGCGGTCATGGAAGCTTTA+TGG 0.228420 1:-23055739 MsG0180001547.01.T01:CDS
AACCACCTTGGTCTTGGTTT+CGG 0.234364 1:+23055296 None:intergenic
TGGTGAGCCATTCCACATTT+TGG 0.235817 1:-23055455 MsG0180001547.01.T01:CDS
CGCGGGAACCACCTGAGTTT+TGG 0.257404 1:+23056178 None:intergenic
CGCGGGAACCACGTGAGTTT+TGG 0.262453 1:+23056391 None:intergenic
TATTGCTGCTGAGTGAGTTT+TGG 0.264260 1:+23056005 None:intergenic
CGCGGGGACCACGTGAGTTT+TGG 0.286625 1:+23056604 None:intergenic
AGTGCAAACCTGAGCCTTAT+TGG 0.298943 1:+23055762 None:intergenic
GCTGCCACGGATCAGGCTTC+TGG 0.299114 1:-23055337 MsG0180001547.01.T01:CDS
ACAAGCTGCAAAGACATATT+TGG 0.303194 1:-23055608 MsG0180001547.01.T01:CDS
ACCGGCTGGAGGACTCTTCC+AGG 0.311413 1:-23056486 MsG0180001547.01.T01:CDS
ACCGGTGGGAGGACTCTTCC+AGG 0.316656 1:-23056273 MsG0180001547.01.T01:CDS
TCCTGCTCAGCTGCAGCCTC+AGG 0.321152 1:+23055361 None:intergenic
TTGGAGAACCACCTTGGTCT+TGG 0.365263 1:+23055290 None:intergenic
GGCGTTGGTCTTCTAGTAGC+TGG 0.367779 1:-23055718 MsG0180001547.01.T01:CDS
TTTCGGGTGCCACCTTGATC+TGG 0.372774 1:+23055313 None:intergenic
ATGAGAATATAATTCGGAAT+TGG 0.380773 1:+23055936 None:intergenic
GCAGGATGCTGCCACGGATC+AGG 0.390421 1:-23055344 MsG0180001547.01.T01:CDS
CATTGTTCAGCTCGCCAATA+AGG 0.391041 1:-23055776 MsG0180001547.01.T01:CDS
ACGAGAAGATAATTCGGAAT+TGG 0.397771 1:+23056355 None:intergenic
ACGAGAAGATAATTCGGAAT+TGG 0.397771 1:+23056568 None:intergenic
CGCTTGGGCAGTTGTAATAG+AGG 0.407257 1:+23055674 None:intergenic
TCAAGATTGATGATCACATT+GGG 0.410067 1:-23055900 MsG0180001547.01.T01:CDS
GCTGCAGCCTCAGGTTCAGC+AGG 0.411570 1:+23055370 None:intergenic
AAGCAAGGTTCAGCAGCAAT+AGG 0.412149 1:-23056036 MsG0180001547.01.T01:CDS
CACATTGGGGTCGCCATCGC+TGG 0.422767 1:-23055886 MsG0180001547.01.T01:CDS
ACCTGAGGCTGCAGCTGAGC+AGG 0.426509 1:-23055362 MsG0180001547.01.T01:CDS
AAAACTCACTCAGCAGCAAT+AGG 0.441532 1:-23056003 MsG0180001547.01.T01:CDS
CTTCTGACGAGAAGATAATT+CGG 0.442067 1:+23056349 None:intergenic
CTTCTGACGAGAAGATAATT+CGG 0.442067 1:+23056562 None:intergenic
CATCCTTGATCAGATCTTCC+CGG 0.452984 1:+23055554 None:intergenic
ATAGGCGGGAGGACTCTTCC+AGG 0.453829 1:-23056082 MsG0180001547.01.T01:intron
CCTTGCAAGGTGAAAAGCTC+AGG 0.463589 1:-23055510 MsG0180001547.01.T01:CDS
TTAAATTATCAAATGTCCAT+TGG 0.471688 1:+23055271 None:intergenic
TCATGGAAGCTTTATGGCGT+TGG 0.472468 1:-23055733 MsG0180001547.01.T01:CDS
AGGTTTGCACTCAGCGGTCA+TGG 0.472469 1:-23055750 MsG0180001547.01.T01:CDS
GATCAGATCTTCCCGGGAGG+AGG 0.476998 1:+23055561 None:intergenic
CGTCTGATGAGAATATAATT+CGG 0.478624 1:+23055930 None:intergenic
TGAACAATGAGACGACCAAC+GGG 0.483551 1:+23055790 None:intergenic
ATTCAAAATAGTTTCCGCTT+GGG 0.489662 1:+23055659 None:intergenic
AGCTGAGCAGGATGCTGCCA+CGG 0.494167 1:-23055350 MsG0180001547.01.T01:CDS
ATGAGACGACCAACGGGAAG+CGG 0.494920 1:+23055796 None:intergenic
ACAACTTCACAGCCTCCTCC+CGG 0.497935 1:-23055573 MsG0180001547.01.T01:CDS
GAATATCAGGCATTTGCAAT+CGG 0.498552 1:-23055640 MsG0180001547.01.T01:CDS
GTCATGGCCGTGTTCTTTCG+CGG 0.498669 1:-23055864 MsG0180001547.01.T01:CDS
GGGGTCGCCATCGCTGGTCA+TGG 0.501187 1:-23055880 MsG0180001547.01.T01:CDS
CTTCCAGGTGGAATATGCCT+TGG 0.501451 1:-23056258 MsG0180001547.01.T01:CDS
CTTCCAGGTGGAATATGCCT+TGG 0.501451 1:-23056471 MsG0180001547.01.T01:CDS
TCAGCTCGCCAATAAGGCTC+AGG 0.504921 1:-23055770 MsG0180001547.01.T01:CDS
TATTCAAAATAGTTTCCGCT+TGG 0.508568 1:+23055658 None:intergenic
TCAATACGCGGGAGACGCCA+TGG 0.512832 1:-23056666 MsG0180001547.01.T01:CDS
ACCATTGCTGTGGTTGGAGT+TGG 0.514251 1:-23055475 MsG0180001547.01.T01:CDS
CAGGCTTCTGGTCCAGATCA+AGG 0.516092 1:-23055325 MsG0180001547.01.T01:CDS
TTCAAGATTGATGATCACAT+TGG 0.525957 1:-23055901 MsG0180001547.01.T01:CDS
TATGAATCTCCGCTTCCCGT+TGG 0.529000 1:-23055805 MsG0180001547.01.T01:CDS
AGGATGCACTCATTGCAACT+AGG 0.531575 1:-23055537 MsG0180001547.01.T01:CDS
ACCATGTTGCTACGTCGGTA+TGG 0.535044 1:+23056297 None:intergenic
ACCATGTTGCTACGTCGGTA+TGG 0.535044 1:+23056510 None:intergenic
AACCACAGCAATGGTGCATA+CGG 0.536605 1:+23055483 None:intergenic
CTTCCAAGGCATATTCCACC+TGG 0.538816 1:+23056255 None:intergenic
CTTCCAAGGCATATTCCACC+TGG 0.538816 1:+23056468 None:intergenic
CTCTATTACAACTGCCCAAG+CGG 0.540260 1:-23055673 MsG0180001547.01.T01:CDS
CATGGCGTATTCCGCTTCCA+AGG 0.545988 1:+23056241 None:intergenic
CATGGCGTATTCCGCTTCCA+AGG 0.545988 1:+23056454 None:intergenic
GGATGCACTCATTGCAACTA+GGG 0.552459 1:-23055536 MsG0180001547.01.T01:CDS
GTAGCAACATGGTCACCGGC+TGG 0.553541 1:-23056500 MsG0180001547.01.T01:CDS
GTATGCACCATTGCTGTGGT+TGG 0.557714 1:-23055481 MsG0180001547.01.T01:CDS
ATCCTTGATCAGATCTTCCC+GGG 0.560166 1:+23055555 None:intergenic
ACCTTGCTTAACTGCTTCCA+TGG 0.560856 1:+23056436 None:intergenic
ACCTTGCTTAACTGCTTCCA+TGG 0.560856 1:+23056649 None:intergenic
CTTCCATGGAGTATTTCACC+TGG 0.566584 1:+23056064 None:intergenic
CTTCCAGGTGAAATACTCCA+TGG 0.566879 1:-23056067 MsG0180001547.01.T01:CDS
CTCCCGGGAAGATCTGATCA+AGG 0.567548 1:-23055557 MsG0180001547.01.T01:CDS
ACCTGGAAGAGTCCTCCAGC+CGG 0.568522 1:+23056485 None:intergenic
ACCTTGCTTAAACGCTTCCA+TGG 0.571915 1:+23056050 None:intergenic
TCATGTACCGCGAAAGAACA+CGG 0.573227 1:+23055857 None:intergenic
TTCTCGTCAGAAGAAGATCT+CGG 0.574567 1:-23056339 MsG0180001547.01.T01:CDS
TTCTCGTCAGAAGAAGATCT+CGG 0.574567 1:-23056552 MsG0180001547.01.T01:CDS
ATCTTGCGTAACTGCTTCCA+TGG 0.575974 1:+23056223 None:intergenic
AGACCAAGGTGGTTCTCCAA+TGG 0.576426 1:-23055287 MsG0180001547.01.T01:CDS
TCCTGATCCAAAATGTGGAA+TGG 0.578837 1:+23055448 None:intergenic
CTGAACAATGAGACGACCAA+CGG 0.580571 1:+23055789 None:intergenic
CAGTTTCCTGATCCAAAATG+TGG 0.582560 1:+23055443 None:intergenic
AACACGGCCATGACCAGCGA+TGG 0.584927 1:+23055873 None:intergenic
AGCTGCACCTGCTGAACCTG+AGG 0.586152 1:-23055377 MsG0180001547.01.T01:CDS
CAACTTCACAGCCTCCTCCC+GGG 0.586785 1:-23055572 MsG0180001547.01.T01:CDS
GGTGGAATATGCCTTGGAAG+CGG 0.587051 1:-23056252 MsG0180001547.01.T01:CDS
GGTGGAATATGCCTTGGAAG+CGG 0.587051 1:-23056465 MsG0180001547.01.T01:CDS
CGACGTAGCAACATGGTCAC+CGG 0.588574 1:-23056504 MsG0180001547.01.T01:CDS
ACCTGGAAGAGTCCTCCCAC+CGG 0.593931 1:+23056272 None:intergenic
ACCAACTCCAACCACAGCAA+TGG 0.594452 1:+23055474 None:intergenic
TCCATGGAAGCGTTTAAGCA+AGG 0.596864 1:-23056051 MsG0180001547.01.T01:CDS
CGACGTAGCAACATGGTCAC+CGG 0.600442 1:-23056291 MsG0180001547.01.T01:CDS
CGTAGCAACATGGTCACCGG+TGG 0.609902 1:-23056288 MsG0180001547.01.T01:CDS
CTCCGTATGCACCATTGCTG+TGG 0.613046 1:-23055485 MsG0180001547.01.T01:CDS
ACCATACCGACGTAGCAACA+TGG 0.615289 1:-23056298 MsG0180001547.01.T01:CDS
TGTCCATTGGAGAACCACCT+TGG 0.616691 1:+23055284 None:intergenic
GCAACTAGGGAGTCCTTGCA+AGG 0.618439 1:-23055523 MsG0180001547.01.T01:CDS
AGCTGGATTAGATGAATCAG+TGG 0.626539 1:-23055701 MsG0180001547.01.T01:CDS
AGATCTCCGATCCAAAACTC+AGG 0.639155 1:-23056189 MsG0180001547.01.T01:intron
TTGGCTTTCTTAACACACGC+GGG 0.639669 1:+23056587 None:intergenic
ATGTATAGAAATCAATACGC+GGG 0.644086 1:-23056677 MsG0180001547.01.T01:CDS
CGGTGACCATGTTGCTACGT+CGG 0.646877 1:+23056292 None:intergenic
GCAACATGGTCACCGGCTGG+AGG 0.648676 1:-23056497 MsG0180001547.01.T01:CDS
ACCATACCGACGTAGCAACA+TGG 0.656046 1:-23056511 MsG0180001547.01.T01:CDS
GCCATGGAAGCAGTTAAGCA+AGG 0.656151 1:-23056437 MsG0180001547.01.T01:CDS
GCCATGGAAGCAGTTAAGCA+AGG 0.656151 1:-23056650 MsG0180001547.01.T01:CDS
CGGTGACCATGTTGCTACGT+CGG 0.658556 1:+23056505 None:intergenic
GGCACCCGAAACCAAGACCA+AGG 0.661446 1:-23055301 MsG0180001547.01.T01:CDS
TGGCTTTCTTAACACACGCG+GGG 0.664879 1:+23056588 None:intergenic
GCAACATGGTCACCGGTGGG+AGG 0.664926 1:-23056284 MsG0180001547.01.T01:CDS
CTTGGAAGCGGAATACGCCA+TGG 0.668490 1:-23056240 MsG0180001547.01.T01:CDS
CTTGGAAGCGGAATACGCCA+TGG 0.668490 1:-23056453 MsG0180001547.01.T01:CDS
CAAGATTGATGATCACATTG+GGG 0.671177 1:-23055899 MsG0180001547.01.T01:CDS
TTGGCTTTGTTAACACACGC+GGG 0.677360 1:+23056374 None:intergenic
GGCTGGAGGACTCTTCCAGG+TGG 0.679444 1:-23056483 MsG0180001547.01.T01:CDS
TGGACCAGAAGCCTGATCCG+TGG 0.681580 1:+23055333 None:intergenic
ATTGGCTTTGTTAACACACG+CGG 0.684897 1:+23056373 None:intergenic
GTAGCAACATGGTCACCGGT+GGG 0.689507 1:-23056287 MsG0180001547.01.T01:CDS
AGGCTCAGGTTTGCACTCAG+CGG 0.694438 1:-23055756 MsG0180001547.01.T01:CDS
ATTGGCTTTCTTAACACACG+CGG 0.703749 1:+23056586 None:intergenic
AAATCAATACCATAGGCGGG+AGG 0.721618 1:-23056093 MsG0180001547.01.T01:intron
TCTCCGATCCAAAACTCACG+TGG 0.728289 1:-23055980 MsG0180001547.01.T01:CDS
TCTCCGATCCAAAACTCACG+TGG 0.728289 1:-23056399 MsG0180001547.01.T01:CDS
CTTGATCAGATCTTCCCGGG+AGG 0.728540 1:+23055558 None:intergenic
GGTGGGAGGACTCTTCCAGG+TGG 0.732528 1:-23056270 MsG0180001547.01.T01:CDS
TCTACGATCCAAAACTCACG+TGG 0.758814 1:-23056612 MsG0180001547.01.T01:CDS
ACCCGAAACCAAGACCAAGG+TGG 0.764375 1:-23055298 MsG0180001547.01.T01:CDS
TATGTATAGAAATCAATACG+CGG 0.767399 1:-23056678 MsG0180001547.01.T01:CDS
GCTTCTGGTCCAGATCAAGG+TGG 0.783084 1:-23055322 MsG0180001547.01.T01:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!!! GTATTGATTTCTAAACATTT+AGG + Chr1:23055853-23055872 None:intergenic 20.0%
! ATGAGAATATAATTCGGAAT+TGG + Chr1:23056023-23056042 None:intergenic 25.0%
! TGTTTAGAAATCAATACCAT+AGG - Chr1:23055856-23055875 MsG0180001547.01.T01:CDS 25.0%
ATGTATAGAAATCAATACGC+GGG - Chr1:23055279-23055298 MsG0180001547.01.T01:CDS 30.0%
ATTCAAAATAGTTTCCGCTT+GGG + Chr1:23056300-23056319 None:intergenic 30.0%
CGTCTGATGAGAATATAATT+CGG + Chr1:23056029-23056048 None:intergenic 30.0%
TATTCAAAATAGTTTCCGCT+TGG + Chr1:23056301-23056320 None:intergenic 30.0%
TTAGAAATCAATACCATAGG+CGG - Chr1:23055859-23055878 MsG0180001547.01.T01:CDS 30.0%
! TCAAGATTGATGATCACATT+GGG - Chr1:23056056-23056075 MsG0180001547.01.T01:CDS 30.0%
! TTCAAGATTGATGATCACAT+TGG - Chr1:23056055-23056074 MsG0180001547.01.T01:CDS 30.0%
!! CGGAAACTATTTTGAATATC+AGG - Chr1:23056303-23056322 MsG0180001547.01.T01:CDS 30.0%
!!! TGATACTTTTGAGATTGTGA+GGG - Chr1:23056549-23056568 MsG0180001547.01.T01:CDS 30.0%
!!! TTGATACTTTTGAGATTGTG+AGG - Chr1:23056548-23056567 MsG0180001547.01.T01:CDS 30.0%
ACAAGCTGCAAAGACATATT+TGG - Chr1:23056348-23056367 MsG0180001547.01.T01:CDS 35.0%
ACGAGAAGATAATTCGGAAT+TGG + Chr1:23055391-23055410 None:intergenic 35.0%
CTTCTGACGAGAAGATAATT+CGG + Chr1:23055397-23055416 None:intergenic 35.0%
GAATATCAGGCATTTGCAAT+CGG - Chr1:23056316-23056335 MsG0180001547.01.T01:CDS 35.0%
TAGAAATCAATACCATAGGC+GGG - Chr1:23055860-23055879 MsG0180001547.01.T01:CDS 35.0%
! CAAGATTGATGATCACATTG+GGG - Chr1:23056057-23056076 MsG0180001547.01.T01:CDS 35.0%
AAAACTCACTCAGCAGCAAT+AGG - Chr1:23055953-23055972 MsG0180001547.01.T01:CDS 40.0%
CAGTTTCCTGATCCAAAATG+TGG + Chr1:23056516-23056535 None:intergenic 40.0%
TCCTGATCCAAAATGTGGAA+TGG + Chr1:23056511-23056530 None:intergenic 40.0%
TTCTCGTCAGAAGAAGATCT+CGG - Chr1:23055404-23055423 MsG0180001547.01.T01:CDS 40.0%
! AGCTGGATTAGATGAATCAG+TGG - Chr1:23056255-23056274 MsG0180001547.01.T01:CDS 40.0%
! TATTGCTGCTGAGTGAGTTT+TGG + Chr1:23055954-23055973 None:intergenic 40.0%
!! ATTGGCTTTCTTAACACACG+CGG + Chr1:23055373-23055392 None:intergenic 40.0%
!! ATTGGCTTTGTTAACACACG+CGG + Chr1:23055586-23055605 None:intergenic 40.0%
!!! TACTTTTGAGATTGTGAGGG+AGG - Chr1:23056552-23056571 MsG0180001547.01.T01:CDS 40.0%
AAATCAATACCATAGGCGGG+AGG - Chr1:23055863-23055882 MsG0180001547.01.T01:CDS 45.0%
AACCACAGCAATGGTGCATA+CGG + Chr1:23056476-23056495 None:intergenic 45.0%
AAGCAAGGTTCAGCAGCAAT+AGG - Chr1:23055534-23055553 MsG0180001547.01.T01:CDS 45.0%
ACCTTGCTTAAACGCTTCCA+TGG + Chr1:23055909-23055928 None:intergenic 45.0%
ACCTTGCTTAACTGCTTCCA+TGG + Chr1:23055310-23055329 None:intergenic 45.0%
AGATCTCCGATCCAAAACTC+AGG - Chr1:23055554-23055573 MsG0180001547.01.T01:CDS 45.0%
AGGATGCACTCATTGCAACT+AGG - Chr1:23056419-23056438 MsG0180001547.01.T01:CDS 45.0%
ATCCTTGATCAGATCTTCCC+GGG + Chr1:23056404-23056423 None:intergenic 45.0%
ATCTTGCGTAACTGCTTCCA+TGG + Chr1:23055736-23055755 None:intergenic 45.0%
CATCCTTGATCAGATCTTCC+CGG + Chr1:23056405-23056424 None:intergenic 45.0%
CATTGTTCAGCTCGCCAATA+AGG - Chr1:23056180-23056199 MsG0180001547.01.T01:intron 45.0%
CTCTATTACAACTGCCCAAG+CGG - Chr1:23056283-23056302 MsG0180001547.01.T01:CDS 45.0%
CTGAACAATGAGACGACCAA+CGG + Chr1:23056170-23056189 None:intergenic 45.0%
CTTCCAGGTGAAATACTCCA+TGG - Chr1:23055889-23055908 MsG0180001547.01.T01:CDS 45.0%
GGATGCACTCATTGCAACTA+GGG - Chr1:23056420-23056439 MsG0180001547.01.T01:CDS 45.0%
TCATGTACCGCGAAAGAACA+CGG + Chr1:23056102-23056121 None:intergenic 45.0%
TCTACGATCCAAAACTCACG+TGG - Chr1:23055344-23055363 MsG0180001547.01.T01:CDS 45.0%
TGAACAATGAGACGACCAAC+GGG + Chr1:23056169-23056188 None:intergenic 45.0%
! AGTGCAAACCTGAGCCTTAT+TGG + Chr1:23056197-23056216 None:intergenic 45.0%
! CTTCCATGGAGTATTTCACC+TGG + Chr1:23055895-23055914 None:intergenic 45.0%
! GCCATTCCACATTTTGGATC+AGG - Chr1:23056507-23056526 MsG0180001547.01.T01:CDS 45.0%
! TCATGGAAGCTTTATGGCGT+TGG - Chr1:23056223-23056242 MsG0180001547.01.T01:CDS 45.0%
! TGGTGAGCCATTCCACATTT+TGG - Chr1:23056501-23056520 MsG0180001547.01.T01:CDS 45.0%
! TTGGCTTTCTTAACACACGC+GGG + Chr1:23055372-23055391 None:intergenic 45.0%
!! AACCACCTTGGTCTTGGTTT+CGG + Chr1:23056663-23056682 None:intergenic 45.0%
!! TCCATGGAAGCGTTTAAGCA+AGG - Chr1:23055905-23055924 MsG0180001547.01.T01:CDS 45.0%
!! TTGGCTTTGTTAACACACGC+GGG + Chr1:23055585-23055604 None:intergenic 45.0%
!!! CTGCTGAGTGAGTTTTGGAT+CGG + Chr1:23055949-23055968 None:intergenic 45.0%
!!! GAACCACCTGAGTTTTGGAT+CGG + Chr1:23055776-23055795 None:intergenic 45.0%
!!! GAACCACGTGAGTTTTGGAT+CGG + Chr1:23055563-23055582 None:intergenic 45.0%
ACCAACTCCAACCACAGCAA+TGG + Chr1:23056485-23056504 None:intergenic 50.0%
ACCATACCGACGTAGCAACA+TGG - Chr1:23055445-23055464 MsG0180001547.01.T01:CDS 50.0%
ACCATGTTGCTACGTCGGTA+TGG + Chr1:23055449-23055468 None:intergenic 50.0%
ACCATTGCTGTGGTTGGAGT+TGG - Chr1:23056481-23056500 MsG0180001547.01.T01:CDS 50.0%
AGACCAAGGTGGTTCTCCAA+TGG - Chr1:23056669-23056688 MsG0180001547.01.T01:CDS 50.0%
CAGCGGTCATGGAAGCTTTA+TGG - Chr1:23056217-23056236 MsG0180001547.01.T01:CDS 50.0%
CCTTGCAAGGTGAAAAGCTC+AGG - Chr1:23056446-23056465 MsG0180001547.01.T01:CDS 50.0%
CGCTTGGGCAGTTGTAATAG+AGG + Chr1:23056285-23056304 None:intergenic 50.0%
CTTCCAAGGCATATTCCACC+TGG + Chr1:23055491-23055510 None:intergenic 50.0%
CTTCCAGGTGGAATATGCCT+TGG - Chr1:23055485-23055504 MsG0180001547.01.T01:CDS 50.0%
GCCATGGAAGCAGTTAAGCA+AGG - Chr1:23055306-23055325 MsG0180001547.01.T01:CDS 50.0%
GGTGGAATATGCCTTGGAAG+CGG - Chr1:23055491-23055510 MsG0180001547.01.T01:CDS 50.0%
GTATGCACCATTGCTGTGGT+TGG - Chr1:23056475-23056494 MsG0180001547.01.T01:CDS 50.0%
TATGAATCTCCGCTTCCCGT+TGG - Chr1:23056151-23056170 MsG0180001547.01.T01:intron 50.0%
TCTCCGATCCAAAACTCACG+TGG - Chr1:23055557-23055576 MsG0180001547.01.T01:CDS 50.0%
TCTCCGATCCAAAACTCAGG+TGG - Chr1:23055770-23055789 MsG0180001547.01.T01:CDS 50.0%
TGGCTTTCTTAACACACGCG+GGG + Chr1:23055371-23055390 None:intergenic 50.0%
TGTCCATTGGAGAACCACCT+TGG + Chr1:23056675-23056694 None:intergenic 50.0%
TTGGAGAACCACCTTGGTCT+TGG + Chr1:23056669-23056688 None:intergenic 50.0%
! CCTGAGCTTTTCACCTTGCA+AGG + Chr1:23056449-23056468 None:intergenic 50.0%
!! ACCACCTTGGTCTTGGTTTC+GGG + Chr1:23056662-23056681 None:intergenic 50.0%
ACAACTTCACAGCCTCCTCC+CGG - Chr1:23056383-23056402 MsG0180001547.01.T01:CDS 55.0%
ACCCGAAACCAAGACCAAGG+TGG - Chr1:23056658-23056677 MsG0180001547.01.T01:CDS 55.0%
AGGCTCAGGTTTGCACTCAG+CGG - Chr1:23056200-23056219 MsG0180001547.01.T01:CDS 55.0%
AGGTTTGCACTCAGCGGTCA+TGG - Chr1:23056206-23056225 MsG0180001547.01.T01:CDS 55.0%
ATGAGACGACCAACGGGAAG+CGG + Chr1:23056163-23056182 None:intergenic 55.0%
CAGGCTTCTGGTCCAGATCA+AGG - Chr1:23056631-23056650 MsG0180001547.01.T01:CDS 55.0%
CATGGCGTATTCCGCTTCCA+AGG + Chr1:23055505-23055524 None:intergenic 55.0%
CGACGTAGCAACATGGTCAC+CGG - Chr1:23055452-23055471 MsG0180001547.01.T01:CDS 55.0%
CGGTGACCATGTTGCTACGT+CGG + Chr1:23055454-23055473 None:intergenic 55.0%
CTCCCGGGAAGATCTGATCA+AGG - Chr1:23056399-23056418 MsG0180001547.01.T01:CDS 55.0%
CTCCGTATGCACCATTGCTG+TGG - Chr1:23056471-23056490 MsG0180001547.01.T01:CDS 55.0%
CTTGATCAGATCTTCCCGGG+AGG + Chr1:23056401-23056420 None:intergenic 55.0%
CTTGGAAGCGGAATACGCCA+TGG - Chr1:23055503-23055522 MsG0180001547.01.T01:CDS 55.0%
GCAACTAGGGAGTCCTTGCA+AGG - Chr1:23056433-23056452 MsG0180001547.01.T01:CDS 55.0%
GCTTCTGGTCCAGATCAAGG+TGG - Chr1:23056634-23056653 MsG0180001547.01.T01:CDS 55.0%
GTAGCAACATGGTCACCGGT+GGG - Chr1:23055669-23055688 MsG0180001547.01.T01:CDS 55.0%
GTCATGGCCGTGTTCTTTCG+CGG - Chr1:23056092-23056111 MsG0180001547.01.T01:intron 55.0%
TCAGCTCGCCAATAAGGCTC+AGG - Chr1:23056186-23056205 MsG0180001547.01.T01:intron 55.0%
! CGCGAGAACCACGTGAGTTT+TGG + Chr1:23055987-23056006 None:intergenic 55.0%
! TTTCGGGTGCCACCTTGATC+TGG + Chr1:23056646-23056665 None:intergenic 55.0%
!! GGCGTTGGTCTTCTAGTAGC+TGG - Chr1:23056238-23056257 MsG0180001547.01.T01:CDS 55.0%
AACACGGCCATGACCAGCGA+TGG + Chr1:23056086-23056105 None:intergenic 60.0%
ACCTGGAAGAGTCCTCCAGC+CGG + Chr1:23055474-23055493 None:intergenic 60.0%
ACCTGGAAGAGTCCTCCCAC+CGG + Chr1:23055687-23055706 None:intergenic 60.0%
AGCTGAGCAGGATGCTGCCA+CGG - Chr1:23056606-23056625 MsG0180001547.01.T01:CDS 60.0%
AGCTGCACCTGCTGAACCTG+AGG - Chr1:23056579-23056598 MsG0180001547.01.T01:CDS 60.0%
ATAGGCGGGAGGACTCTTCC+AGG - Chr1:23055874-23055893 MsG0180001547.01.T01:CDS 60.0%
CAACTTCACAGCCTCCTCCC+GGG - Chr1:23056384-23056403 MsG0180001547.01.T01:CDS 60.0%
CGTAGCAACATGGTCACCGG+TGG - Chr1:23055455-23055474 MsG0180001547.01.T01:CDS 60.0%
GATCAGATCTTCCCGGGAGG+AGG + Chr1:23056398-23056417 None:intergenic 60.0%
GGCACCCGAAACCAAGACCA+AGG - Chr1:23056655-23056674 MsG0180001547.01.T01:CDS 60.0%
GTAGCAACATGGTCACCGGC+TGG - Chr1:23055456-23055475 MsG0180001547.01.T01:CDS 60.0%
TCAATACGCGGGAGACGCCA+TGG - Chr1:23055290-23055309 MsG0180001547.01.T01:CDS 60.0%
TGGAAGAGTCCTCCCGCCTA+TGG + Chr1:23055875-23055894 None:intergenic 60.0%
TGGACCAGAAGCCTGATCCG+TGG + Chr1:23056626-23056645 None:intergenic 60.0%
! CGCGGGAACCACCTGAGTTT+TGG + Chr1:23055781-23055800 None:intergenic 60.0%
! CGCGGGAACCACGTGAGTTT+TGG + Chr1:23055568-23055587 None:intergenic 60.0%
ACCGGCTGGAGGACTCTTCC+AGG - Chr1:23055470-23055489 MsG0180001547.01.T01:CDS 65.0%
ACCGGTGGGAGGACTCTTCC+AGG - Chr1:23055683-23055702 MsG0180001547.01.T01:CDS 65.0%
ACCTGAGGCTGCAGCTGAGC+AGG - Chr1:23056594-23056613 MsG0180001547.01.T01:CDS 65.0%
CACATTGGGGTCGCCATCGC+TGG - Chr1:23056070-23056089 MsG0180001547.01.T01:CDS 65.0%
GCAACATGGTCACCGGTGGG+AGG - Chr1:23055672-23055691 MsG0180001547.01.T01:CDS 65.0%
GCAGGATGCTGCCACGGATC+AGG - Chr1:23056612-23056631 MsG0180001547.01.T01:CDS 65.0%
GCTGCAGCCTCAGGTTCAGC+AGG + Chr1:23056589-23056608 None:intergenic 65.0%
GCTGCCACGGATCAGGCTTC+TGG - Chr1:23056619-23056638 MsG0180001547.01.T01:CDS 65.0%
GGCTGGAGGACTCTTCCAGG+TGG - Chr1:23055473-23055492 MsG0180001547.01.T01:CDS 65.0%
GGTGGGAGGACTCTTCCAGG+TGG - Chr1:23055686-23055705 MsG0180001547.01.T01:CDS 65.0%
TCCTGCTCAGCTGCAGCCTC+AGG + Chr1:23056598-23056617 None:intergenic 65.0%
! CGCGGGGACCACGTGAGTTT+TGG + Chr1:23055355-23055374 None:intergenic 65.0%
! GCAACATGGTCACCGGCTGG+AGG - Chr1:23055459-23055478 MsG0180001547.01.T01:CDS 65.0%
GGGGTCGCCATCGCTGGTCA+TGG - Chr1:23056076-23056095 MsG0180001547.01.T01:CDS 70.0%
Chromosome Type Strat End Strand Name
Chr1 gene 23055279 23056699 23055279 ID=MsG0180001547.01;Name=MsG0180001547.01
Chr1 mRNA 23055279 23056699 23055279 ID=MsG0180001547.01.T01;Parent=MsG0180001547.01;Name=MsG0180001547.01.T01;_AED=0.21;_eAED=0.21;_QI=0|0|0|1|0|0|2|0|443
Chr1 exon 23056190 23056699 23056190 ID=MsG0180001547.01.T01:exon:40401;Parent=MsG0180001547.01.T01
Chr1 exon 23055279 23056100 23055279 ID=MsG0180001547.01.T01:exon:40400;Parent=MsG0180001547.01.T01
Chr1 CDS 23056190 23056699 23056190 ID=MsG0180001547.01.T01:cds;Parent=MsG0180001547.01.T01
Chr1 CDS 23055279 23056100 23055279 ID=MsG0180001547.01.T01:cds;Parent=MsG0180001547.01.T01
Gene Sequence

>MsG0180001547.01.T01

ATGTATAGAAATCAATACGCGGGAGACGCCATGGAAGCAGTTAAGCAAGGTTCAGCAACAATAGATCTACGATCCAAAACTCACGTGGTCCCCGCGTGTGTTAAGAAAGCCAATTCCGAATTATCTTCTCGTCAGAAGAAGATCTCGGAGATGCTTAGAAATCAATACCATACCGACGTAGCAACATGGTCACCGGCTGGAGGACTCTTCCAGGTGGAATATGCCTTGGAAGCGGAATACGCCATGGAAGCAGTTAAGCAAGGTTCAGCAGCAATAGATCTCCGATCCAAAACTCACGTGGTTCCCGCGTGTGTTAACAAAGCCAATTCCGAATTATCTTCTCGTCAGAAGAAGATCTCGGAGATGCTTAGAAATCGATACCATACCGACGTAGCAACATGGTCACCGGTGGGAGGACTCTTCCAGGTGGAATATGCCTTGGAAGCGGAATACGCCATGGAAGCAGTTACGCAAGATTCAGCAGCAATAGATCTCCGATCCAAAACTCAGGCGGGAGGACTCTTCCAGGTGAAATACTCCATGGAAGCGTTTAAGCAAGGTTCAGCAGCAATAGGTCTCCGATCCAAAACTCACTCAGCAGCAATAGGTCTCCGATCCAAAACTCACGTGGTTCTCGCGTGTGTTAACAAAGCCAATTCCGAATTATATTCTCATCAGACGAAGATCTTCAAGATTGATGATCACATTGGGGTCGCCATCGCTGGTCATGGCCGTGTTCTTTCGCGGTACATGAGATCTGAATGCATAAACTATAATTACACTTATGAATCTCCGCTTCCCGTTGGTCGTCTCATTGTTCAGCTCGCCAATAAGGCTCAGGTTTGCACTCAGCGGTCATGGAAGCTTTATGGCGTTGGTCTTCTAGTAGCTGGATTAGATGAATCAGTGGCTCACCTCTATTACAACTGCCCAAGCGGAAACTATTTTGAATATCAGGCATTTGCAATCGGTTCATGCTCACAAGCTGCAAAGACATATTTGGAACGCAAATTTAACAACTTCACAGCCTCCTCCCGGGAAGATCTGATCAAGGATGCACTCATTGCAACTAGGGAGTCCTTGCAAGGTGAAAAGCTCAGGATCTCCGTATGCACCATTGCTGTGGTTGGAGTTGGTGAGCCATTCCACATTTTGGATCAGGAAACTGTTCAACAATTGATTGATACTTTTGAGATTGTGAGGGAGGAAGAAGCTGCACCTGCTGAACCTGAGGCTGCAGCTGAGCAGGATGCTGCCACGGATCAGGCTTCTGGTCCAGATCAAGGTGGCACCCGAAACCAAGACCAAGGTGGTTCTCCAATGGACATTTGA
Protein sequence

>MsG0180001547.01.T01

MYRNQYAGDAMEAVKQGSATIDLRSKTHVVPACVKKANSELSSRQKKISEMLRNQYHTDVATWSPAGGLFQVEYALEAEYAMEAVKQGSAAIDLRSKTHVVPACVNKANSELSSRQKKISEMLRNRYHTDVATWSPVGGLFQVEYALEAEYAMEAVTQDSAAIDLRSKTQAGGLFQVKYSMEAFKQGSAAIGLRSKTHSAAIGLRSKTHVVLACVNKANSELYSHQTKIFKIDDHIGVAIAGHGRVLSRYMRSECINYNYTYESPLPVGRLIVQLANKAQVCTQRSWKLYGVGLLVAGLDESVAHLYYNCPSGNYFEYQAFAIGSCSQAAKTYLERKFNNFTASSREDLIKDALIATRESLQGEKLRISVCTIAVVGVGEPFHILDQETVQQLIDTFEIVREEEAAPAEPEAAAEQDAATDQASGPDQGGTRNQDQGGSPMDI*