Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0880047266.01.T01 | XP_003609890.1 | 71.658 | 187 | 20 | 3 | 1 | 155 | 1 | 186 | 9.17E-48 | 167 |
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0880047266.01.T01 | G7JR20 | 71.658 | 187 | 20 | 3 | 1 | 155 | 1 | 186 | 4.38e-48 | 167 |
| Gene ID | Type | Classification |
|---|
| Gene ID | Type | Classification |
|---|
Co-expression Network
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MsG0080048016.01 | MsG0880047266.01 | 0.812561 | 3.984387e-51 | 2.541611e-48 |
| MsG0180001064.01 | MsG0880047266.01 | 0.808175 | 3.524523e-50 | 2.001443e-47 |
| MsG0180001100.01 | MsG0880047266.01 | 0.823524 | 1.322299e-53 | 1.141338e-50 |
| MsG0880044145.01 | MsG0880047266.01 | 0.803301 | 3.722222e-49 | 1.863832e-46 |
| MsG0880047266.01 | MsG0880047267.01 | 0.885275 | 9.358044e-72 | 6.383367e-68 |
| MsG0880047266.01 | MsG0880047519.01 | 0.832400 | 9.658473e-56 | 1.078354e-52 |
| MsG0380014538.01 | MsG0880047266.01 | 0.823109 | 1.652921e-53 | 1.410145e-50 |
| MsG0380014727.01 | MsG0880047266.01 | 0.820845 | 5.531208e-53 | 4.426375e-50 |
| MsG0380014866.01 | MsG0880047266.01 | 0.812740 | 3.641129e-51 | 2.333610e-48 |
| MsG0680034235.01 | MsG0880047266.01 | 0.821517 | 3.870803e-53 | 3.155951e-50 |
| MsG0480020024.01 | MsG0880047266.01 | 0.800114 | 1.677437e-48 | 7.746490e-46 |
| MsG0480022624.01 | MsG0880047266.01 | 0.826432 | 2.722137e-54 | 2.552487e-51 |
| MsG0480023565.01 | MsG0880047266.01 | 0.800755 | 1.241814e-48 | 5.828741e-46 |
| MsG0580024775.01 | MsG0880047266.01 | 0.809517 | 1.819509e-50 | 1.070534e-47 |
| MsG0380016740.01 | MsG0880047266.01 | 0.803826 | 2.896562e-49 | 1.470171e-46 |
| MsG0380017201.01 | MsG0880047266.01 | 0.821932 | 3.104332e-53 | 2.561022e-50 |
| MsG0580028033.01 | MsG0880047266.01 | 0.830279 | 3.210963e-55 | 3.367412e-52 |
| MsG0680030515.01 | MsG0880047266.01 | 0.807757 | 4.324503e-50 | 2.428761e-47 |
| MsG0280008738.01 | MsG0880047266.01 | 0.801814 | 7.537721e-49 | 3.634216e-46 |
| MsG0280008932.01 | MsG0880047266.01 | 0.814071 | 1.856406e-51 | 1.233322e-48 |
| MsG0780037330.01 | MsG0880047266.01 | 0.805817 | 1.111592e-49 | 5.938588e-47 |
| MsG0780037701.01 | MsG0880047266.01 | 0.849227 | 3.702996e-60 | 6.973441e-57 |
PPI
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0880047266.01.T01 | MTR_4g124080 | 71.658 | 187 | 20 | 3 | 1 | 155 | 1 | 186 | 1.11e-51 | 167 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
Find 43 sgRNAs with CRISPR-Local
Find 71 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| ACAATAACAACCACCGAAAA+AGG | 0.244300 | 8:+83975247 | MsG0880047266.01.T01:five_prime_UTR |
| TCGGAGAGTGAGGGTGTCTA+TGG | 0.311445 | 8:+83975409 | MsG0880047266.01.T01:CDS |
| AATGAAACAAGAGAAGAAAA+AGG | 0.361365 | 8:+83975912 | MsG0880047266.01.T01:CDS |
| CCATTCATCCACCGGCTTTG+TGG | 0.383430 | 8:-83976034 | None:intergenic |
| GTCATCGTCATGGAGGTTTC+CGG | 0.398566 | 8:+83975317 | MsG0880047266.01.T01:exon |
| GAAAGTAAGAGCAGGGGAGA+AGG | 0.401972 | 8:+83975876 | MsG0880047266.01.T01:CDS |
| GTGAGGGTGTCTATGGGGTC+TGG | 0.444353 | 8:+83975416 | MsG0880047266.01.T01:CDS |
| CTTCAATTCCATTCATCCAC+CGG | 0.448619 | 8:-83976042 | None:intergenic |
| CCACAAAGCCGGTGGATGAA+TGG | 0.449970 | 8:+83976034 | MsG0880047266.01.T01:CDS |
| AGGAACAACGCCATGAGAAC+GGG | 0.451237 | 8:-83975387 | None:intergenic |
| CGGAGAGTGAGGGTGTCTAT+GGG | 0.456153 | 8:+83975410 | MsG0880047266.01.T01:CDS |
| GCTTCTGAATACCTCACTTA+GGG | 0.462381 | 8:+83975550 | MsG0880047266.01.T01:CDS |
| GTTCTCATGGCGTTGTTCCT+CGG | 0.476868 | 8:+83975390 | MsG0880047266.01.T01:CDS |
| CAACCTACATCTCCGGCGTT+GGG | 0.481801 | 8:+83975357 | MsG0880047266.01.T01:CDS |
| GAGCTAGAGAAAGTAAGAGC+AGG | 0.491556 | 8:+83975868 | MsG0880047266.01.T01:CDS |
| CGCTTCTGAATACCTCACTT+AGG | 0.493514 | 8:+83975549 | MsG0880047266.01.T01:CDS |
| GGGGTCTGGTTTCCATGATG+AGG | 0.505395 | 8:+83975430 | MsG0880047266.01.T01:CDS |
| ACAACCTACATCTCCGGCGT+TGG | 0.506506 | 8:+83975356 | MsG0880047266.01.T01:CDS |
| GGGTCTGGTTTCCATGATGA+GGG | 0.513644 | 8:+83975431 | MsG0880047266.01.T01:CDS |
| GGGACTCACACCCGTTCTCA+TGG | 0.519048 | 8:+83975377 | MsG0880047266.01.T01:CDS |
| AGTCCCAACGCCGGAGATGT+AGG | 0.532391 | 8:-83975360 | None:intergenic |
| GACTATCACCGTCATCGTCA+TGG | 0.541050 | 8:+83975307 | MsG0880047266.01.T01:exon |
| AGCTAGAGAAAGTAAGAGCA+GGG | 0.546581 | 8:+83975869 | MsG0880047266.01.T01:CDS |
| GCGTTGTTCCTCGGAGAGTG+AGG | 0.551861 | 8:+83975399 | MsG0880047266.01.T01:CDS |
| ACTGCACGTGACCCTCATCA+TGG | 0.571166 | 8:-83975442 | None:intergenic |
| ATCACTTGACTCAGATTCAG+AGG | 0.579827 | 8:-83975995 | None:intergenic |
| GAGGAACAACGCCATGAGAA+CGG | 0.580124 | 8:-83975388 | None:intergenic |
| TGTACGACGGAGAACGGTGC+CGG | 0.582122 | 8:-83975336 | None:intergenic |
| CGTTGTTCCTCGGAGAGTGA+GGG | 0.586366 | 8:+83975400 | MsG0880047266.01.T01:CDS |
| CGTCGTACAACCTACATCTC+CGG | 0.602217 | 8:+83975350 | MsG0880047266.01.T01:CDS |
| GTAGGTTGTACGACGGAGAA+CGG | 0.604515 | 8:-83975342 | None:intergenic |
| TTACATGAATAGATGCAGGA+TGG | 0.624402 | 8:+83975826 | MsG0880047266.01.T01:intron |
| GGAAACCTCCATGACGATGA+CGG | 0.624471 | 8:-83975315 | None:intergenic |
| GCTAGAGAAAGTAAGAGCAG+GGG | 0.626526 | 8:+83975870 | MsG0880047266.01.T01:CDS |
| TTGTGATGTTGCCACAAAGC+CGG | 0.644781 | 8:+83976023 | MsG0880047266.01.T01:CDS |
| TGATGTTGCCACAAAGCCGG+TGG | 0.654844 | 8:+83976026 | MsG0880047266.01.T01:CDS |
| ACGGGTGTGAGTCCCAACGC+CGG | 0.672451 | 8:-83975369 | None:intergenic |
| GGAGAGTGAGGGTGTCTATG+GGG | 0.673193 | 8:+83975411 | MsG0880047266.01.T01:CDS |
| TGTTGAGCAAACCCTAAGTG+AGG | 0.684457 | 8:-83975561 | None:intergenic |
| ATAGACACCCTCACTCTCCG+AGG | 0.695788 | 8:-83975407 | None:intergenic |
| CATGAATAGATGCAGGATGG+TGG | 0.702277 | 8:+83975829 | MsG0880047266.01.T01:intron |
| TATCACCGTCATCGTCATGG+AGG | 0.734877 | 8:+83975310 | MsG0880047266.01.T01:exon |
| CGGAGATGTAGGTTGTACGA+CGG | 0.747412 | 8:-83975349 | None:intergenic |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !! | ATAATAAAATAAAAGAAAAG+GGG | - | Chr8:83975645-83975664 | None:intergenic | 10.0% |
| !! | GAATTTGTGGTATAAATAAA+GGG | - | Chr8:83975752-83975771 | None:intergenic | 20.0% |
| !! | TGAATTTGTGGTATAAATAA+AGG | - | Chr8:83975753-83975772 | None:intergenic | 20.0% |
| !!! | TTTATTACAAGTTTTCATCT+TGG | - | Chr8:83975953-83975972 | None:intergenic | 20.0% |
| ! | AATGAAACAAGAGAAGAAAA+AGG | + | Chr8:83975912-83975931 | MsG0880047266.01.T01:CDS | 25.0% |
| ! | CATCATCATATTTGAATTTG+TGG | - | Chr8:83975765-83975784 | None:intergenic | 25.0% |
| !!! | CAAAGTATTGTTTCCTTTTT+CGG | - | Chr8:83975263-83975282 | None:intergenic | 25.0% |
| !!! | TTTTTTACATGAATAGATGC+AGG | + | Chr8:83975822-83975841 | MsG0880047266.01.T01:intron | 25.0% |
| !!! | TTTTTTTCCTGTTTCTTGTT+GGG | + | Chr8:83975718-83975737 | MsG0880047266.01.T01:intron | 25.0% |
| GATCTTTATTCAGAATCTGA+AGG | - | Chr8:83975694-83975713 | None:intergenic | 30.0% | |
| ! | ATTACAAGTTTTCATCTTGG+AGG | - | Chr8:83975950-83975969 | None:intergenic | 30.0% |
| ! | TTACAAGTTTTCATCTTGGA+GGG | - | Chr8:83975949-83975968 | None:intergenic | 30.0% |
| !!! | GTTTTTTTCCTGTTTCTTGT+TGG | + | Chr8:83975717-83975736 | MsG0880047266.01.T01:intron | 30.0% |
| !!! | TTTTTTCCTGTTTCTTGTTG+GGG | + | Chr8:83975719-83975738 | MsG0880047266.01.T01:intron | 30.0% |
| ACAATAACAACCACCGAAAA+AGG | + | Chr8:83975247-83975266 | MsG0880047266.01.T01:five_prime_UTR | 35.0% | |
| CCTAATCTACCTAATCTCTT+GGG | + | Chr8:83975585-83975604 | MsG0880047266.01.T01:intron | 35.0% | |
| TCCTAATCTACCTAATCTCT+TGG | + | Chr8:83975584-83975603 | MsG0880047266.01.T01:intron | 35.0% | |
| TTACATGAATAGATGCAGGA+TGG | + | Chr8:83975826-83975845 | MsG0880047266.01.T01:intron | 35.0% | |
| !! | TTTTTCCTGTTTCTTGTTGG+GGG | + | Chr8:83975720-83975739 | MsG0880047266.01.T01:intron | 35.0% |
| !!! | AGGTATTCAGAAGCGTTTTT+AGG | - | Chr8:83975544-83975563 | None:intergenic | 35.0% |
| !!! | AGTATTGTTTCCTTTTTCGG+TGG | - | Chr8:83975260-83975279 | None:intergenic | 35.0% |
| !!! | CTGTTTTTTTCTTCACGTCT+TGG | - | Chr8:83975472-83975491 | None:intergenic | 35.0% |
| AGCTAGAGAAAGTAAGAGCA+GGG | + | Chr8:83975869-83975888 | MsG0880047266.01.T01:CDS | 40.0% | |
| ATCACTTGACTCAGATTCAG+AGG | - | Chr8:83975998-83976017 | None:intergenic | 40.0% | |
| CCCAAGAGATTAGGTAGATT+AGG | - | Chr8:83975588-83975607 | None:intergenic | 40.0% | |
| GCTTCTGAATACCTCACTTA+GGG | + | Chr8:83975550-83975569 | MsG0880047266.01.T01:CDS | 40.0% | |
| TGAAGTTGACCCAAGAGATT+AGG | - | Chr8:83975597-83975616 | None:intergenic | 40.0% | |
| !! | AAAAAAGTACCGTGAGGCTT+TGG | - | Chr8:83975620-83975639 | None:intergenic | 40.0% |
| !! | TTTTCCTGTTTCTTGTTGGG+GGG | + | Chr8:83975721-83975740 | MsG0880047266.01.T01:intron | 40.0% |
| CATGAATAGATGCAGGATGG+TGG | + | Chr8:83975829-83975848 | MsG0880047266.01.T01:intron | 45.0% | |
| CGCTTCTGAATACCTCACTT+AGG | + | Chr8:83975549-83975568 | MsG0880047266.01.T01:CDS | 45.0% | |
| GAGCTAGAGAAAGTAAGAGC+AGG | + | Chr8:83975868-83975887 | MsG0880047266.01.T01:CDS | 45.0% | |
| GCTAGAGAAAGTAAGAGCAG+GGG | + | Chr8:83975870-83975889 | MsG0880047266.01.T01:CDS | 45.0% | |
| TCAACTTCACCAAAGCCTCA+CGG | + | Chr8:83975608-83975627 | MsG0880047266.01.T01:intron | 45.0% | |
| TGTTGAGCAAACCCTAAGTG+AGG | - | Chr8:83975564-83975583 | None:intergenic | 45.0% | |
| TTGTGATGTTGCCACAAAGC+CGG | + | Chr8:83976023-83976042 | MsG0880047266.01.T01:CDS | 45.0% | |
| ! | TTTCCTGTTTCTTGTTGGGG+GGG | + | Chr8:83975722-83975741 | MsG0880047266.01.T01:intron | 45.0% |
| !! | AAATAATAAAATAAAAGAAA+AGG | - | Chr8:83975647-83975666 | None:intergenic | 5.0% |
| !! | AATAATAAAATAAAAGAAAA+GGG | - | Chr8:83975646-83975665 | None:intergenic | 5.0% |
| AAACCCCCCCAACAAGAAAC+AGG | - | Chr8:83975728-83975747 | None:intergenic | 50.0% | |
| AGGAACAACGCCATGAGAAC+GGG | - | Chr8:83975390-83975409 | None:intergenic | 50.0% | |
| CGGAGATGTAGGTTGTACGA+CGG | - | Chr8:83975352-83975371 | None:intergenic | 50.0% | |
| CGTCGTACAACCTACATCTC+CGG | + | Chr8:83975350-83975369 | MsG0880047266.01.T01:CDS | 50.0% | |
| GAAAGTAAGAGCAGGGGAGA+AGG | + | Chr8:83975876-83975895 | MsG0880047266.01.T01:CDS | 50.0% | |
| GACTATCACCGTCATCGTCA+TGG | + | Chr8:83975307-83975326 | MsG0880047266.01.T01:exon | 50.0% | |
| GAGGAACAACGCCATGAGAA+CGG | - | Chr8:83975391-83975410 | None:intergenic | 50.0% | |
| GGAAACCTCCATGACGATGA+CGG | - | Chr8:83975318-83975337 | None:intergenic | 50.0% | |
| GTAGGTTGTACGACGGAGAA+CGG | - | Chr8:83975345-83975364 | None:intergenic | 50.0% | |
| GTCATCGTCATGGAGGTTTC+CGG | + | Chr8:83975317-83975336 | MsG0880047266.01.T01:exon | 50.0% | |
| GTTCTCATGGCGTTGTTCCT+CGG | + | Chr8:83975390-83975409 | MsG0880047266.01.T01:CDS | 50.0% | |
| TATCACCGTCATCGTCATGG+AGG | + | Chr8:83975310-83975329 | MsG0880047266.01.T01:exon | 50.0% | |
| ! | GGGTCTGGTTTCCATGATGA+GGG | + | Chr8:83975431-83975450 | MsG0880047266.01.T01:CDS | 50.0% |
| !! | GGGGTGAAAAAAGTACCGTG+AGG | - | Chr8:83975626-83975645 | None:intergenic | 50.0% |
| ACAACCTACATCTCCGGCGT+TGG | + | Chr8:83975356-83975375 | MsG0880047266.01.T01:CDS | 55.0% | |
| ACTGCACGTGACCCTCATCA+TGG | - | Chr8:83975445-83975464 | None:intergenic | 55.0% | |
| ATAGACACCCTCACTCTCCG+AGG | - | Chr8:83975410-83975429 | None:intergenic | 55.0% | |
| CAACCTACATCTCCGGCGTT+GGG | + | Chr8:83975357-83975376 | MsG0880047266.01.T01:CDS | 55.0% | |
| CCACAAAGCCGGTGGATGAA+TGG | + | Chr8:83976034-83976053 | MsG0880047266.01.T01:CDS | 55.0% | |
| CGTTGTTCCTCGGAGAGTGA+GGG | + | Chr8:83975400-83975419 | MsG0880047266.01.T01:CDS | 55.0% | |
| TGATGTTGCCACAAAGCCGG+TGG | + | Chr8:83976026-83976045 | MsG0880047266.01.T01:CDS | 55.0% | |
| ! | GGGGTCTGGTTTCCATGATG+AGG | + | Chr8:83975430-83975449 | MsG0880047266.01.T01:CDS | 55.0% |
| !! | CCATTCATCCACCGGCTTTG+TGG | - | Chr8:83976037-83976056 | None:intergenic | 55.0% |
| !! | CGGAGAGTGAGGGTGTCTAT+GGG | + | Chr8:83975410-83975429 | MsG0880047266.01.T01:CDS | 55.0% |
| !! | GGAGAGTGAGGGTGTCTATG+GGG | + | Chr8:83975411-83975430 | MsG0880047266.01.T01:CDS | 55.0% |
| !! | TCGGAGAGTGAGGGTGTCTA+TGG | + | Chr8:83975409-83975428 | MsG0880047266.01.T01:CDS | 55.0% |
| AGTCCCAACGCCGGAGATGT+AGG | - | Chr8:83975363-83975382 | None:intergenic | 60.0% | |
| GCGTTGTTCCTCGGAGAGTG+AGG | + | Chr8:83975399-83975418 | MsG0880047266.01.T01:CDS | 60.0% | |
| GGGACTCACACCCGTTCTCA+TGG | + | Chr8:83975377-83975396 | MsG0880047266.01.T01:CDS | 60.0% | |
| TGTACGACGGAGAACGGTGC+CGG | - | Chr8:83975339-83975358 | None:intergenic | 60.0% | |
| !! | GTGAGGGTGTCTATGGGGTC+TGG | + | Chr8:83975416-83975435 | MsG0880047266.01.T01:CDS | 60.0% |
| ACGGGTGTGAGTCCCAACGC+CGG | - | Chr8:83975372-83975391 | None:intergenic | 65.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| Chr8 | gene | 83975235 | 83976062 | 83975235 | ID=MsG0880047266.01;Name=MsG0880047266.01 |
| Chr8 | mRNA | 83975235 | 83976062 | 83975235 | ID=MsG0880047266.01.T01;Parent=MsG0880047266.01;Name=MsG0880047266.01.T01;_AED=0.48;_eAED=0.48;_QI=91|1|1|1|1|1|2|0|156 |
| Chr8 | exon | 83975235 | 83975571 | 83975235 | ID=MsG0880047266.01.T01:exon:35843;Parent=MsG0880047266.01.T01 |
| Chr8 | exon | 83975838 | 83976062 | 83975838 | ID=MsG0880047266.01.T01:exon:35844;Parent=MsG0880047266.01.T01 |
| Chr8 | five_prime_UTR | 83975235 | 83975325 | 83975235 | ID=MsG0880047266.01.T01:five_prime_utr;Parent=MsG0880047266.01.T01 |
| Chr8 | CDS | 83975326 | 83975571 | 83975326 | ID=MsG0880047266.01.T01:cds;Parent=MsG0880047266.01.T01 |
| Chr8 | CDS | 83975838 | 83976062 | 83975838 | ID=MsG0880047266.01.T01:cds;Parent=MsG0880047266.01.T01 |
| Gene Sequence |
| Protein sequence |
