AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar ZhongmuNo.1 / MsG0680030515.01


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsG0680030515.01.T01 MTR_8g432340 93.487 261 17 0 1 261 1 261 8.92e-163 466
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsG0680030515.01.T01 AT2G31890 53.107 177 82 1 86 261 102 278 1.77e-31 122

Find 62 sgRNAs with CRISPR-Local

Find 67 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
CGCCAAGTTCTCGTGGTAAC+TGG 0.239282 6:+4461703 None:intergenic
ATAGAAGATTTACTTGATTA+TGG 0.247712 6:-4461274 MsG0680030515.01.T01:CDS
GGAAGGGTTATTGAATTCTT+TGG 0.291031 6:-4461782 MsG0680030515.01.T01:CDS
CTTCTCAAATCACTTTCCTT+TGG 0.312687 6:+4461223 None:intergenic
GGCTTATGCGATGGAAGATT+TGG 0.313416 6:-4461380 MsG0680030515.01.T01:CDS
ATCAGAGTTTGTCGGGGAAT+TGG 0.338938 6:-4461536 MsG0680030515.01.T01:CDS
TATGGTTCAGAGGAAGAGTT+TGG 0.340010 6:-4461256 MsG0680030515.01.T01:CDS
AATAGACTGTCTCAATTCTC+TGG 0.368929 6:-4461133 MsG0680030515.01.T01:CDS
TAACGGAGAGGAGTCTGATA+TGG 0.415258 6:-4461566 MsG0680030515.01.T01:CDS
CAAAGGGTTTAAAACAACTT+TGG 0.423201 6:+4461755 None:intergenic
AGGAGATAGATTGGCTGCAT+TGG 0.425081 6:-4461599 MsG0680030515.01.T01:CDS
GTTACCGAGCTCCGAAGAAA+AGG 0.425877 6:-4461504 MsG0680030515.01.T01:CDS
ATTGGGAATCAGAGTTTGTC+GGG 0.442092 6:-4461543 MsG0680030515.01.T01:CDS
GTCGGGGAATTGGATCCGTT+TGG 0.444529 6:-4461526 MsG0680030515.01.T01:CDS
GAGAGGAGTCTGATATGGAT+TGG 0.456624 6:-4461561 MsG0680030515.01.T01:CDS
TCCCAGTTACCACGAGAACT+TGG 0.459982 6:-4461705 MsG0680030515.01.T01:CDS
TGAAATGTTTGCAGAAAATA+CGG 0.460460 6:-4461179 MsG0680030515.01.T01:CDS
GGGAAATTATAACTAACTCT+TGG 0.464356 6:+4461724 None:intergenic
AAATCACTTTCCTTTGGCTG+TGG 0.472714 6:+4461229 None:intergenic
AACAATTGTCGCGGTTGCAA+AGG 0.476557 6:-4461023 MsG0680030515.01.T01:CDS
AGAGGAGTCTGATATGGATT+GGG 0.487748 6:-4461560 MsG0680030515.01.T01:CDS
AAAGAAGGTTGAGGAGAAGG+TGG 0.499984 6:-4461332 MsG0680030515.01.T01:CDS
TTGCAGAAAATACGGAGAAA+AGG 0.502813 6:-4461171 MsG0680030515.01.T01:CDS
GCCAAGTTCTCGTGGTAACT+GGG 0.523815 6:+4461704 None:intergenic
TTGGTGTGTGAGGGCGAGAG+AGG 0.528486 6:-4461434 MsG0680030515.01.T01:CDS
TTCGGAGCTCGGTAACCAAA+CGG 0.529938 6:+4461511 None:intergenic
GATAGTAAAGCCGGTGCTAG+AGG 0.534515 6:-4461619 MsG0680030515.01.T01:CDS
ACTCTTGGAGTGAAACCAAA+GGG 0.537314 6:+4461739 None:intergenic
CGGTGCTAGAGGAGATAGAT+TGG 0.539783 6:-4461608 MsG0680030515.01.T01:CDS
GTTTGGACTGCCACAGCCAA+AGG 0.542148 6:-4461239 MsG0680030515.01.T01:CDS
GATTGGGAATCAGAGTTTGT+CGG 0.543250 6:-4461544 MsG0680030515.01.T01:CDS
AATCGATTAAAGCGAAGGGT+AGG 0.547508 6:-4461402 MsG0680030515.01.T01:CDS
ATAGACTGTCTCAATTCTCT+GGG 0.548337 6:-4461132 MsG0680030515.01.T01:CDS
CAATCTATCTCCTCTAGCAC+CGG 0.560589 6:+4461609 None:intergenic
TTACCGAGCTCCGAAGAAAA+GGG 0.560642 6:-4461503 MsG0680030515.01.T01:CDS
GCTTGAGGATACCGATGGAA+TGG 0.566585 6:-4461458 MsG0680030515.01.T01:CDS
TTTGATAGAGATAGTAAAGC+CGG 0.570770 6:-4461628 MsG0680030515.01.T01:CDS
ATTTAAACGCCAAGTTCTCG+TGG 0.571729 6:+4461696 None:intergenic
GGATAATTGAAAAGTATGGA+AGG 0.572370 6:-4461799 None:intergenic
ATCGATTAAAGCGAAGGGTA+GGG 0.575049 6:-4461401 MsG0680030515.01.T01:CDS
TTTACTTGATTATGGTTCAG+AGG 0.575949 6:-4461266 MsG0680030515.01.T01:CDS
GATAGATTGGCTGCATTGGA+TGG 0.577186 6:-4461595 MsG0680030515.01.T01:CDS
CAAAGGAAAGTGATTTGAGA+AGG 0.581889 6:-4461222 MsG0680030515.01.T01:CDS
ATTGAAATCGATTAAAGCGA+AGG 0.591251 6:-4461407 MsG0680030515.01.T01:CDS
GATAATTGAAAAGTATGGAA+GGG 0.604646 6:-4461798 None:intergenic
AGAAAATACGGAGAAAAGGA+TGG 0.607622 6:-4461167 MsG0680030515.01.T01:CDS
AACTCTTGGAGTGAAACCAA+AGG 0.610647 6:+4461738 None:intergenic
GAAGGGTAGGGCTTATGCGA+TGG 0.611487 6:-4461389 MsG0680030515.01.T01:CDS
GCAAAAGTCGAAGTTGCTTG+AGG 0.618329 6:-4461473 MsG0680030515.01.T01:CDS
GCATTGGATGGTGATGATAA+CGG 0.618404 6:-4461583 MsG0680030515.01.T01:CDS
GCAGACTGCAGATGATGTGT+TGG 0.621746 6:-4461059 MsG0680030515.01.T01:CDS
AGGATACCGATGGAATGGAT+TGG 0.626596 6:-4461453 MsG0680030515.01.T01:CDS
CACACACCAATCCATTCCAT+CGG 0.628347 6:+4461447 None:intergenic
TTGAAATCGATTAAAGCGAA+GGG 0.635470 6:-4461406 MsG0680030515.01.T01:CDS
ATGGAATGGATTGGTGTGTG+AGG 0.635612 6:-4461444 MsG0680030515.01.T01:CDS
TGGAATGGATTGGTGTGTGA+GGG 0.643530 6:-4461443 MsG0680030515.01.T01:CDS
GTGTGTGAGGGCGAGAGAGG+TGG 0.645809 6:-4461431 MsG0680030515.01.T01:CDS
TACATACGAAACAATTGTCG+CGG 0.653812 6:-4461032 MsG0680030515.01.T01:CDS
AAGTTGCTTGAGGATACCGA+TGG 0.678693 6:-4461463 MsG0680030515.01.T01:CDS
TTGGGAATCAGAGTTTGTCG+GGG 0.682626 6:-4461542 MsG0680030515.01.T01:CDS
AAGGCAAGTGAGTTTGATTG+CGG 0.684765 6:-4461203 MsG0680030515.01.T01:CDS
GGATGGTGATGATAACGGAG+AGG 0.710792 6:-4461578 MsG0680030515.01.T01:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! ATAGAAGATTTACTTGATTA+TGG - Chr6:4461528-4461547 MsG0680030515.01.T01:CDS 20.0%
! GAAGAAGAAAAAGAAAAAGA+AGG - Chr6:4461455-4461474 MsG0680030515.01.T01:CDS 25.0%
! TGAAATGTTTGCAGAAAATA+CGG - Chr6:4461623-4461642 MsG0680030515.01.T01:CDS 25.0%
!!! TCAAGTTGATTTCTTTTCTA+TGG + Chr6:4461704-4461723 None:intergenic 25.0%
ATTGAAATCGATTAAAGCGA+AGG - Chr6:4461395-4461414 MsG0680030515.01.T01:CDS 30.0%
GAAAAAGAAAAAGAAGGTTG+AGG - Chr6:4461461-4461480 MsG0680030515.01.T01:CDS 30.0%
GGGAAATTATAACTAACTCT+TGG + Chr6:4461081-4461100 None:intergenic 30.0%
TTGAAATCGATTAAAGCGAA+GGG - Chr6:4461396-4461415 MsG0680030515.01.T01:CDS 30.0%
TTTACTTGATTATGGTTCAG+AGG - Chr6:4461536-4461555 MsG0680030515.01.T01:CDS 30.0%
TTTGATAGAGATAGTAAAGC+CGG - Chr6:4461174-4461193 MsG0680030515.01.T01:CDS 30.0%
! CAAAGGGTTTAAAACAACTT+TGG + Chr6:4461050-4461069 None:intergenic 30.0%
!!! CAAAGTTGTTTTAAACCCTT+TGG - Chr6:4461048-4461067 MsG0680030515.01.T01:CDS 30.0%
AATAGACTGTCTCAATTCTC+TGG - Chr6:4461669-4461688 MsG0680030515.01.T01:CDS 35.0%
AGAAAATACGGAGAAAAGGA+TGG - Chr6:4461635-4461654 MsG0680030515.01.T01:CDS 35.0%
ATAGACTGTCTCAATTCTCT+GGG - Chr6:4461670-4461689 MsG0680030515.01.T01:CDS 35.0%
CAAAGGAAAGTGATTTGAGA+AGG - Chr6:4461580-4461599 MsG0680030515.01.T01:CDS 35.0%
CTTCTCAAATCACTTTCCTT+TGG + Chr6:4461582-4461601 None:intergenic 35.0%
GGAAGGGTTATTGAATTCTT+TGG - Chr6:4461020-4461039 MsG0680030515.01.T01:CDS 35.0%
TACATACGAAACAATTGTCG+CGG - Chr6:4461770-4461789 MsG0680030515.01.T01:CDS 35.0%
TTGCAGAAAATACGGAGAAA+AGG - Chr6:4461631-4461650 MsG0680030515.01.T01:CDS 35.0%
! TGCTCTTTTTCCCTTTTCTT+CGG + Chr6:4461312-4461331 None:intergenic 35.0%
AAATCACTTTCCTTTGGCTG+TGG + Chr6:4461576-4461595 None:intergenic 40.0%
AACTCTTGGAGTGAAACCAA+AGG + Chr6:4461067-4461086 None:intergenic 40.0%
AAGGCAAGTGAGTTTGATTG+CGG - Chr6:4461599-4461618 MsG0680030515.01.T01:CDS 40.0%
AATCGATTAAAGCGAAGGGT+AGG - Chr6:4461400-4461419 MsG0680030515.01.T01:CDS 40.0%
ACTCTTGGAGTGAAACCAAA+GGG + Chr6:4461066-4461085 None:intergenic 40.0%
AGAGGAGTCTGATATGGATT+GGG - Chr6:4461242-4461261 MsG0680030515.01.T01:CDS 40.0%
ATCGATTAAAGCGAAGGGTA+GGG - Chr6:4461401-4461420 MsG0680030515.01.T01:CDS 40.0%
ATTGGGAATCAGAGTTTGTC+GGG - Chr6:4461259-4461278 MsG0680030515.01.T01:CDS 40.0%
ATTTAAACGCCAAGTTCTCG+TGG + Chr6:4461109-4461128 None:intergenic 40.0%
GAAAAAGAAGGTTGAGGAGA+AGG - Chr6:4461467-4461486 MsG0680030515.01.T01:CDS 40.0%
GATTGGGAATCAGAGTTTGT+CGG - Chr6:4461258-4461277 MsG0680030515.01.T01:CDS 40.0%
TATGGTTCAGAGGAAGAGTT+TGG - Chr6:4461546-4461565 MsG0680030515.01.T01:CDS 40.0%
! GCATTGGATGGTGATGATAA+CGG - Chr6:4461219-4461238 MsG0680030515.01.T01:CDS 40.0%
AAAGAAGGTTGAGGAGAAGG+TGG - Chr6:4461470-4461489 MsG0680030515.01.T01:CDS 45.0%
AACAATTGTCGCGGTTGCAA+AGG - Chr6:4461779-4461798 MsG0680030515.01.T01:CDS 45.0%
AAGTTGCTTGAGGATACCGA+TGG - Chr6:4461339-4461358 MsG0680030515.01.T01:CDS 45.0%
AGGATACCGATGGAATGGAT+TGG - Chr6:4461349-4461368 MsG0680030515.01.T01:CDS 45.0%
ATCAGAGTTTGTCGGGGAAT+TGG - Chr6:4461266-4461285 MsG0680030515.01.T01:CDS 45.0%
CAATCTATCTCCTCTAGCAC+CGG + Chr6:4461196-4461215 None:intergenic 45.0%
CACACACCAATCCATTCCAT+CGG + Chr6:4461358-4461377 None:intergenic 45.0%
GAGAGGAGTCTGATATGGAT+TGG - Chr6:4461241-4461260 MsG0680030515.01.T01:CDS 45.0%
GCAAAAGTCGAAGTTGCTTG+AGG - Chr6:4461329-4461348 MsG0680030515.01.T01:CDS 45.0%
GGCTTATGCGATGGAAGATT+TGG - Chr6:4461422-4461441 MsG0680030515.01.T01:CDS 45.0%
TAACGGAGAGGAGTCTGATA+TGG - Chr6:4461236-4461255 MsG0680030515.01.T01:CDS 45.0%
TTACCGAGCTCCGAAGAAAA+GGG - Chr6:4461299-4461318 MsG0680030515.01.T01:CDS 45.0%
TTGGGAATCAGAGTTTGTCG+GGG - Chr6:4461260-4461279 MsG0680030515.01.T01:CDS 45.0%
! AGGAGATAGATTGGCTGCAT+TGG - Chr6:4461203-4461222 MsG0680030515.01.T01:CDS 45.0%
! GATAGATTGGCTGCATTGGA+TGG - Chr6:4461207-4461226 MsG0680030515.01.T01:CDS 45.0%
! TTTCCCTTTTCTTCGGAGCT+CGG + Chr6:4461305-4461324 None:intergenic 45.0%
!! ATGGAATGGATTGGTGTGTG+AGG - Chr6:4461358-4461377 MsG0680030515.01.T01:CDS 45.0%
!! TGGAATGGATTGGTGTGTGA+GGG - Chr6:4461359-4461378 MsG0680030515.01.T01:CDS 45.0%
GCAGACTGCAGATGATGTGT+TGG - Chr6:4461743-4461762 MsG0680030515.01.T01:CDS 50.0%
GCCAAGTTCTCGTGGTAACT+GGG + Chr6:4461101-4461120 None:intergenic 50.0%
GCTTGAGGATACCGATGGAA+TGG - Chr6:4461344-4461363 MsG0680030515.01.T01:CDS 50.0%
GTTACCGAGCTCCGAAGAAA+AGG - Chr6:4461298-4461317 MsG0680030515.01.T01:CDS 50.0%
TCCCAGTTACCACGAGAACT+TGG - Chr6:4461097-4461116 MsG0680030515.01.T01:CDS 50.0%
TTCGGAGCTCGGTAACCAAA+CGG + Chr6:4461294-4461313 None:intergenic 50.0%
! CGGTGCTAGAGGAGATAGAT+TGG - Chr6:4461194-4461213 MsG0680030515.01.T01:CDS 50.0%
! GATAGTAAAGCCGGTGCTAG+AGG - Chr6:4461183-4461202 MsG0680030515.01.T01:CDS 50.0%
!! GGATGGTGATGATAACGGAG+AGG - Chr6:4461224-4461243 MsG0680030515.01.T01:CDS 50.0%
CGCCAAGTTCTCGTGGTAAC+TGG + Chr6:4461102-4461121 None:intergenic 55.0%
GAAGGGTAGGGCTTATGCGA+TGG - Chr6:4461413-4461432 MsG0680030515.01.T01:CDS 55.0%
GTCGGGGAATTGGATCCGTT+TGG - Chr6:4461276-4461295 MsG0680030515.01.T01:CDS 55.0%
GTTTGGACTGCCACAGCCAA+AGG - Chr6:4461563-4461582 MsG0680030515.01.T01:CDS 55.0%
TTGGTGTGTGAGGGCGAGAG+AGG - Chr6:4461368-4461387 MsG0680030515.01.T01:CDS 60.0%
GTGTGTGAGGGCGAGAGAGG+TGG - Chr6:4461371-4461390 MsG0680030515.01.T01:CDS 65.0%
Chromosome Type Strat End Strand Name
Chr6 gene 4461018 4461806 4461018 ID=MsG0680030515.01;Name=MsG0680030515.01
Chr6 mRNA 4461018 4461806 4461018 ID=MsG0680030515.01.T01;Parent=MsG0680030515.01;Name=MsG0680030515.01.T01;_AED=0.49;_eAED=0.49;_QI=0|-1|0|1|-1|1|1|0|262
Chr6 exon 4461018 4461806 4461018 ID=MsG0680030515.01.T01:exon:12470;Parent=MsG0680030515.01.T01
Chr6 CDS 4461018 4461806 4461018 ID=MsG0680030515.01.T01:cds;Parent=MsG0680030515.01.T01
Gene Sequence

>MsG0680030515.01.T01

ATGGAAGGGTTATTGAATTCTTTGGTTAACCAAAGTTGTTTTAAACCCTTTGGTTTCACTCCAAGAGTTAGTTATAATTTCCCAGTTACCACGAGAACTTGGCGTTTAAATCGAAAACTTGATTCATATAATACATTGAGAATCGATTGCACGCGTTTTGATAGAGATAGTAAAGCCGGTGCTAGAGGAGATAGATTGGCTGCATTGGATGGTGATGATAACGGAGAGGAGTCTGATATGGATTGGGAATCAGAGTTTGTCGGGGAATTGGATCCGTTTGGTTACCGAGCTCCGAAGAAAAGGGAAAAAGAGCAAAAGTCGAAGTTGCTTGAGGATACCGATGGAATGGATTGGTGTGTGAGGGCGAGAGAGGTGGCATTGAAATCGATTAAAGCGAAGGGTAGGGCTTATGCGATGGAAGATTTGGTAACTGTTAAGAAGAAGAAAAAGAAAAAGAAGGTTGAGGAGAAGGTGGTAAGCAAGAAGAAAACTGAGAAGAAAATAGATGAAATAGAAGATTTACTTGATTATGGTTCAGAGGAAGAGTTTGGACTGCCACAGCCAAAGGAAAGTGATTTGAGAAGGCAAGTGAGTTTGATTGCGGATGAAATGTTTGCAGAAAATACGGAGAAAAGGATGGAAACGTTTTACAATAGACTGTCTCAATTCTCTGGGATATCTGACCATAGAAAAGAAATCAACTTGAACAAAGCGATTATTGAATCGCAGACTGCAGATGATGTGTTGGATGTTACATACGAAACAATTGTCGCGGTTGCAAAGGATTGA

Protein sequence

>MsG0680030515.01.T01

MEGLLNSLVNQSCFKPFGFTPRVSYNFPVTTRTWRLNRKLDSYNTLRIDCTRFDRDSKAGARGDRLAALDGDDNGEESDMDWESEFVGELDPFGYRAPKKREKEQKSKLLEDTDGMDWCVRAREVALKSIKAKGRAYAMEDLVTVKKKKKKKKVEEKVVSKKKTEKKIDEIEDLLDYGSEEEFGLPQPKESDLRRQVSLIADEMFAENTEKRMETFYNRLSQFSGISDHRKEINLNKAIIESQTADDVLDVTYETIVAVAKD*