AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar ZhongmuNo.1 / MsG0880047357.01


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsG0880047357.01.T01 MTR_4g125490 99.010 101 1 0 1 101 1 101 2.67e-71 222
MsG0880047357.01.T01 MTR_4g125490 97.727 88 2 0 98 185 217 304 8.90e-54 177
MsG0880047357.01.T01 MTR_2g041430 83.333 102 13 2 4 101 2 103 7.82e-57 182
MsG0880047357.01.T01 MTR_2g041430 58.140 86 27 4 98 176 219 302 6.03e-16 74.7
MsG0880047357.01.T01 MTR_2g041430 83.333 102 13 2 4 101 2 103 8.58e-57 182
MsG0880047357.01.T01 MTR_2g041430 58.140 86 27 4 98 176 219 302 6.43e-16 74.7
MsG0880047357.01.T01 MTR_2g041430 83.333 102 13 2 4 101 2 103 1.01e-56 182
MsG0880047357.01.T01 MTR_2g041430 58.140 86 27 4 98 176 219 302 7.09e-16 74.7
MsG0880047357.01.T01 MTR_8g020560 66.981 106 27 4 1 101 1 103 1.72e-41 143
MsG0880047357.01.T01 MTR_0001s0490 59.574 94 33 2 9 100 25 115 7.62e-32 117
MsG0880047357.01.T01 MTR_5g027030 58.696 92 26 2 9 100 16 95 2.86e-31 115
MsG0880047357.01.T01 MTR_1g017490 52.525 99 41 2 7 100 142 239 1.62e-29 114
MsG0880047357.01.T01 MTR_1g017490 52.525 99 41 2 7 100 9 106 2.02e-29 113
MsG0880047357.01.T01 MTR_7g104050 48.182 110 36 4 6 101 68 170 1.07e-23 96.7
MsG0880047357.01.T01 MTR_7g104050 48.624 109 35 4 6 100 68 169 1.10e-23 96.3
MsG0880047357.01.T01 MTR_3g092330 43.478 92 34 3 9 100 113 186 2.69e-16 76.3
MsG0880047357.01.T01 MTR_8g035910 37.895 95 45 3 6 100 46 126 3.67e-11 61.2
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsG0880047357.01.T01 AT3G13960 71.429 98 22 3 7 101 14 108 8.36e-43 147
MsG0880047357.01.T01 AT2G06200 42.929 198 86 4 7 184 4 194 7.34e-42 141
MsG0880047357.01.T01 AT2G06200 42.714 199 87 4 6 184 42 233 2.22e-41 141
MsG0880047357.01.T01 AT4G37740 55.914 93 37 2 9 100 164 253 1.09e-29 114
MsG0880047357.01.T01 AT2G22840 53.684 95 40 2 7 100 131 222 6.62e-25 100
MsG0880047357.01.T01 AT2G36400 53.608 97 36 3 10 100 77 170 6.90e-24 97.4
MsG0880047357.01.T01 AT3G52910 52.083 96 40 3 10 100 83 177 7.19e-22 91.7
MsG0880047357.01.T01 AT2G06200 40.310 129 59 3 67 184 1 122 1.76e-19 81.6
MsG0880047357.01.T01 AT5G53660 46.237 93 33 2 8 100 58 133 2.18e-18 81.6
MsG0880047357.01.T01 AT4G24150 38.462 104 58 2 2 100 104 206 3.17e-16 75.9
MsG0880047357.01.T01 AT4G24150 38.462 104 58 2 2 100 142 244 3.20e-16 76.3

Find 40 sgRNAs with CRISPR-Local

Find 88 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
TGAAGGAACAGGTATGTTTA+TGG 0.231611 8:+85221621 MsG0880047357.01.T01:intron
TGCCACTGAGTTGGAGTAAA+AGG 0.276188 8:-85220548 None:intergenic
CTTCTGCACCTCCCTGGCTC+TGG 0.278943 8:-85220836 None:intergenic
GCTTTCTTCACTGAACCTTC+TGG 0.302685 8:+85221669 MsG0880047357.01.T01:CDS
CTTTCACAGTACTTTGAATC+TGG 0.319625 8:-85220899 None:intergenic
AAAAGAGGAAGAGGAGCTTA+TGG 0.356828 8:-85221751 None:intergenic
CTTCTTCAATGGATGATTCA+TGG 0.368355 8:+85221712 MsG0880047357.01.T01:CDS
TTCTGCACCTCCCTGGCTCT+GGG 0.368474 8:-85220835 None:intergenic
ATCTACAAATACATGGCTTC+TGG 0.368517 8:+85220593 MsG0880047357.01.T01:CDS
GAACTATTTACCAATGGGAT+TGG 0.400450 8:+85220799 MsG0880047357.01.T01:CDS
TTTAAACAGAGAAATTGTTC+TGG 0.401538 8:+85221771 MsG0880047357.01.T01:CDS
AACTATTTACCAATGGGATT+GGG 0.420553 8:+85220800 MsG0880047357.01.T01:CDS
AAGTTTATCAGCTTCTTCAA+TGG 0.445043 8:+85221701 MsG0880047357.01.T01:CDS
TTGATAGTGAGTAGAAGATC+AGG 0.446923 8:-85220629 None:intergenic
TCTGTTCTTCTGCACCTCCC+TGG 0.454328 8:-85220842 None:intergenic
ACAATTTCTCTGTTTAAAAG+AGG 0.457057 8:-85221766 None:intergenic
GAGTAGAAGATCAGGAGGAA+TGG 0.465599 8:-85220621 None:intergenic
GTAAGAGTACTCATTGTCAT+TGG 0.465700 8:-85221799 None:intergenic
GGATGGAACTATTTACCAAT+GGG 0.479254 8:+85220794 MsG0880047357.01.T01:CDS
CAATGATGAATGGAAGAAAC+AGG 0.479723 8:+85220522 None:intergenic
GAAGAACAGATGGAAAGAAA+TGG 0.480263 8:+85220855 MsG0880047357.01.T01:CDS
GATCAAGGTTGTTACATCTC+AGG 0.490399 8:+85221858 MsG0880047357.01.T01:CDS
TCTCTGTTTAAAAGAGGAAG+AGG 0.498513 8:-85221760 None:intergenic
TAACAAACAACAATGATGAA+TGG 0.501615 8:+85220512 None:intergenic
GTGTGGTGCATGCAGTTGGA+TGG 0.510680 8:+85220777 MsG0880047357.01.T01:intron
TCAAGCTCTTGCCACTGAGT+TGG 0.513392 8:-85220557 None:intergenic
TTATGGACTGAAAGAGGAGG+TGG 0.522907 8:+85221638 MsG0880047357.01.T01:CDS
GAAGAGGAGCTTATGGTAAG+TGG 0.526833 8:-85221744 None:intergenic
AAACAACCACAGCAAGATCA+AGG 0.541674 8:+85221843 MsG0880047357.01.T01:CDS
GAACAGATGGAAAGAAATGG+AGG 0.559737 8:+85220858 MsG0880047357.01.T01:CDS
TGGATGGAACTATTTACCAA+TGG 0.564553 8:+85220793 MsG0880047357.01.T01:CDS
ATAGTGAGTAGAAGATCAGG+AGG 0.571348 8:-85220626 None:intergenic
GGGAGGTGCAGAAGAACAGA+TGG 0.577454 8:+85220845 MsG0880047357.01.T01:CDS
AAACTTCTTATAGTTCCAGA+AGG 0.579842 8:-85221684 None:intergenic
GTATGTTTATGGACTGAAAG+AGG 0.601366 8:+85221632 MsG0880047357.01.T01:CDS
AGCTCTTATCTACAAATACA+TGG 0.606523 8:+85220586 MsG0880047357.01.T01:CDS
TAACAACCTTGATCTTGCTG+TGG 0.626299 8:-85221849 None:intergenic
TGTTTATGGACTGAAAGAGG+AGG 0.650765 8:+85221635 MsG0880047357.01.T01:CDS
AAATAGACCCAGAGCCAGGG+AGG 0.659120 8:+85220828 MsG0880047357.01.T01:CDS
TTACAGTGTTGAGACTGATG+AGG 0.793602 8:-85220683 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! TATATATAGTCATTCATATA+TGG - Chr8:85220724-85220743 None:intergenic 15.0%
!! TAGAGAACTTATAAAATAAG+TGG + Chr8:85221430-85221449 MsG0880047357.01.T01:intron 20.0%
!!! CTTATCGATTATAAACTTAT+AGG - Chr8:85221373-85221392 None:intergenic 20.0%
!!! TTAAGTTGTTTATTCAAACA+TGG - Chr8:85221301-85221320 None:intergenic 20.0%
!!! TTTATGAAAGATTCTCTTTT+TGG + Chr8:85221583-85221602 MsG0880047357.01.T01:intron 20.0%
! ACAATTTCTCTGTTTAAAAG+AGG - Chr8:85221769-85221788 None:intergenic 25.0%
! CTTTAAACCAACACATATTT+AGG + Chr8:85221275-85221294 MsG0880047357.01.T01:intron 25.0%
! TTTAAACAGAGAAATTGTTC+TGG + Chr8:85221771-85221790 MsG0880047357.01.T01:CDS 25.0%
AAACTTCTTATAGTTCCAGA+AGG - Chr8:85221687-85221706 None:intergenic 30.0%
AACTATTTACCAATGGGATT+GGG + Chr8:85220800-85220819 MsG0880047357.01.T01:CDS 30.0%
AAGTTTATCAGCTTCTTCAA+TGG + Chr8:85221701-85221720 MsG0880047357.01.T01:CDS 30.0%
AGCTCTTATCTACAAATACA+TGG + Chr8:85220586-85220605 MsG0880047357.01.T01:CDS 30.0%
TCAAATAGTCCAATCCAAAA+AGG + Chr8:85221522-85221541 MsG0880047357.01.T01:intron 30.0%
TTGTCTTGAAATGAGAAGTT+AGG - Chr8:85221177-85221196 None:intergenic 30.0%
! GGTATTGTTGTTATCTTTGA+AGG + Chr8:85221604-85221623 MsG0880047357.01.T01:intron 30.0%
! TATGTATGCACTTGTTGATT+TGG + Chr8:85220755-85220774 MsG0880047357.01.T01:intron 30.0%
AACATGGCCTAAATATGTGT+TGG - Chr8:85221285-85221304 None:intergenic 35.0%
ACCAGTATTGTCAAGAAACA+AGG - Chr8:85221208-85221227 None:intergenic 35.0%
ATCTACAAATACATGGCTTC+TGG + Chr8:85220593-85220612 MsG0880047357.01.T01:CDS 35.0%
CAATCCAAAAAGGTTCATGA+TGG + Chr8:85221532-85221551 MsG0880047357.01.T01:intron 35.0%
CCAGTATTGTCAAGAAACAA+GGG - Chr8:85221207-85221226 None:intergenic 35.0%
CTTCTTCAATGGATGATTCA+TGG + Chr8:85221712-85221731 MsG0880047357.01.T01:CDS 35.0%
CTTTCACAGTACTTTGAATC+TGG - Chr8:85220902-85220921 None:intergenic 35.0%
GAACTATTTACCAATGGGAT+TGG + Chr8:85220799-85220818 MsG0880047357.01.T01:CDS 35.0%
GAAGAACAGATGGAAAGAAA+TGG + Chr8:85220855-85220874 MsG0880047357.01.T01:CDS 35.0%
GGATGGAACTATTTACCAAT+GGG + Chr8:85220794-85220813 MsG0880047357.01.T01:CDS 35.0%
TCTCTGTTTAAAAGAGGAAG+AGG - Chr8:85221763-85221782 None:intergenic 35.0%
TGAAGGAACAGGTATGTTTA+TGG + Chr8:85221621-85221640 MsG0880047357.01.T01:intron 35.0%
TGGATGGAACTATTTACCAA+TGG + Chr8:85220793-85220812 MsG0880047357.01.T01:CDS 35.0%
! AATAACACAACACTGATTGC+AGG + Chr8:85221240-85221259 MsG0880047357.01.T01:intron 35.0%
! ATTTTTCTTCCCAATCCCAT+TGG - Chr8:85220812-85220831 None:intergenic 35.0%
! CATCATGAACCTTTTTGGAT+TGG - Chr8:85221534-85221553 None:intergenic 35.0%
! GTATGTTTATGGACTGAAAG+AGG + Chr8:85221632-85221651 MsG0880047357.01.T01:CDS 35.0%
! GTTGTTATCTTTGAAGGAAC+AGG + Chr8:85221610-85221629 MsG0880047357.01.T01:intron 35.0%
!! AAGCATAAGAAGAGTGTTGA+GGG - Chr8:85221104-85221123 None:intergenic 35.0%
!! CAGATTCAAAGTACTGTGAA+AGG + Chr8:85220900-85220919 MsG0880047357.01.T01:intron 35.0%
!! GTAAGAGTACTCATTGTCAT+TGG - Chr8:85221802-85221821 None:intergenic 35.0%
!! GTTGTTTTCAAAACTTCCAC+AGG - Chr8:85220959-85220978 None:intergenic 35.0%
!! TTGATAGTGAGTAGAAGATC+AGG - Chr8:85220632-85220651 None:intergenic 35.0%
AAAAGAGGAAGAGGAGCTTA+TGG - Chr8:85221754-85221773 None:intergenic 40.0%
AAACAACCACAGCAAGATCA+AGG + Chr8:85221843-85221862 MsG0880047357.01.T01:CDS 40.0%
AAATGAGAAGTTAGGCCTTG+AGG - Chr8:85221169-85221188 None:intergenic 40.0%
AAGATTGTTGAAGTAGCAGC+AGG - Chr8:85220998-85221017 None:intergenic 40.0%
AAGCTATCGTTGAGACTGAA+TGG + Chr8:85221403-85221422 MsG0880047357.01.T01:intron 40.0%
ATAGTGAGTAGAAGATCAGG+AGG - Chr8:85220629-85220648 None:intergenic 40.0%
GAACAGATGGAAAGAAATGG+AGG + Chr8:85220858-85220877 MsG0880047357.01.T01:CDS 40.0%
GATCAAGGTTGTTACATCTC+AGG + Chr8:85221858-85221877 MsG0880047357.01.T01:CDS 40.0%
TAACAACCTTGATCTTGCTG+TGG - Chr8:85221852-85221871 None:intergenic 40.0%
! AGCACCATCATGAACCTTTT+TGG - Chr8:85221539-85221558 None:intergenic 40.0%
! ATCCTTTTACTCCAACTCAG+TGG + Chr8:85220546-85220565 MsG0880047357.01.T01:CDS 40.0%
! CAGTGTTGTGTTATTGTGAG+AGG - Chr8:85221235-85221254 None:intergenic 40.0%
! CCCTTGTTTCTTGACAATAC+TGG + Chr8:85221204-85221223 MsG0880047357.01.T01:intron 40.0%
! TGTTTATGGACTGAAAGAGG+AGG + Chr8:85221635-85221654 MsG0880047357.01.T01:CDS 40.0%
! TTACAGTGTTGAGACTGATG+AGG - Chr8:85220686-85220705 None:intergenic 40.0%
!! AAGAAGAGTGTTGAGGGTAA+TGG - Chr8:85221098-85221117 None:intergenic 40.0%
!! ATGCACTTGTTGATTTGGTG+TGG + Chr8:85220760-85220779 MsG0880047357.01.T01:intron 40.0%
!! GAAGCATAAGAAGAGTGTTG+AGG - Chr8:85221105-85221124 None:intergenic 40.0%
AAACCGTTCAAGAAAGCCTG+TGG + Chr8:85220940-85220959 MsG0880047357.01.T01:intron 45.0%
AGAAAAATAGACCCAGAGCC+AGG + Chr8:85220824-85220843 MsG0880047357.01.T01:CDS 45.0%
AGAGGAAAGGGTGGTTAAGA+TGG - Chr8:85221131-85221150 None:intergenic 45.0%
CTTCCACAGGCTTTCTTGAA+CGG - Chr8:85220946-85220965 None:intergenic 45.0%
GAAAAATAGACCCAGAGCCA+GGG + Chr8:85220825-85220844 MsG0880047357.01.T01:CDS 45.0%
GAAGAGGAGCTTATGGTAAG+TGG - Chr8:85221747-85221766 None:intergenic 45.0%
GAGGAAAGGGTGGTTAAGAT+GGG - Chr8:85221130-85221149 None:intergenic 45.0%
GAGGGTTATGGTAGAGGAAA+GGG - Chr8:85221143-85221162 None:intergenic 45.0%
GAGTAGAAGATCAGGAGGAA+TGG - Chr8:85220624-85220643 None:intergenic 45.0%
GCTTTCTTCACTGAACCTTC+TGG + Chr8:85221669-85221688 MsG0880047357.01.T01:CDS 45.0%
! GTGAAAGGCACATGCATAGA+GGG + Chr8:85220915-85220934 MsG0880047357.01.T01:intron 45.0%
! TGCCACTGAGTTGGAGTAAA+AGG - Chr8:85220551-85220570 None:intergenic 45.0%
! TGTGAAAGGCACATGCATAG+AGG + Chr8:85220914-85220933 MsG0880047357.01.T01:intron 45.0%
! TTATGGACTGAAAGAGGAGG+TGG + Chr8:85221638-85221657 MsG0880047357.01.T01:CDS 45.0%
!!! ATAAACATTTTAATAAAAAA+AGG - Chr8:85221568-85221587 None:intergenic 5.0%
AAGTTAGGCCTTGAGGATGG+AGG - Chr8:85221162-85221181 None:intergenic 50.0%
ACCATAACCCTCCATCCTCA+AGG + Chr8:85221151-85221170 MsG0880047357.01.T01:intron 50.0%
AGGATGGAGGGTTATGGTAG+AGG - Chr8:85221149-85221168 None:intergenic 50.0%
AGTTAGGCCTTGAGGATGGA+GGG - Chr8:85221161-85221180 None:intergenic 50.0%
GAGAAGTTAGGCCTTGAGGA+TGG - Chr8:85221165-85221184 None:intergenic 50.0%
GGAGGGTTATGGTAGAGGAA+AGG - Chr8:85221144-85221163 None:intergenic 50.0%
GGTTATGGTAGAGGAAAGGG+TGG - Chr8:85221140-85221159 None:intergenic 50.0%
TCAAGCTCTTGCCACTGAGT+TGG - Chr8:85220560-85220579 None:intergenic 50.0%
! TTTGGTGTGGTGCATGCAGT+TGG + Chr8:85220773-85220792 MsG0880047357.01.T01:intron 50.0%
AAATAGACCCAGAGCCAGGG+AGG + Chr8:85220828-85220847 MsG0880047357.01.T01:CDS 55.0%
GCCTTGAGGATGGAGGGTTA+TGG - Chr8:85221155-85221174 None:intergenic 55.0%
GTGTGGTGCATGCAGTTGGA+TGG + Chr8:85220777-85220796 MsG0880047357.01.T01:intron 55.0%
TCTGTTCTTCTGCACCTCCC+TGG - Chr8:85220845-85220864 None:intergenic 55.0%
! GGGAGGTGCAGAAGAACAGA+TGG + Chr8:85220845-85220864 MsG0880047357.01.T01:CDS 55.0%
! TTCTGCACCTCCCTGGCTCT+GGG - Chr8:85220838-85220857 None:intergenic 60.0%
CTTCTGCACCTCCCTGGCTC+TGG - Chr8:85220839-85220858 None:intergenic 65.0%
Chromosome Type Strat End Strand Name
Chr8 gene 85220524 85221899 85220524 ID=MsG0880047357.01;Name=MsG0880047357.01
Chr8 mRNA 85220524 85221899 85220524 ID=MsG0880047357.01.T01;Parent=MsG0880047357.01;Name=MsG0880047357.01.T01;_AED=0.49;_eAED=0.49;_QI=0|0|0|1|1|1|3|0|187
Chr8 exon 85220524 85220701 85220524 ID=MsG0880047357.01.T01:exon:34500;Parent=MsG0880047357.01.T01
Chr8 exon 85220792 85220909 85220792 ID=MsG0880047357.01.T01:exon:34501;Parent=MsG0880047357.01.T01
Chr8 exon 85221632 85221899 85221632 ID=MsG0880047357.01.T01:exon:34502;Parent=MsG0880047357.01.T01
Chr8 CDS 85220524 85220701 85220524 ID=MsG0880047357.01.T01:cds;Parent=MsG0880047357.01.T01
Chr8 CDS 85220792 85220909 85220792 ID=MsG0880047357.01.T01:cds;Parent=MsG0880047357.01.T01
Chr8 CDS 85221632 85221899 85221632 ID=MsG0880047357.01.T01:cds;Parent=MsG0880047357.01.T01
Gene Sequence

>MsG0880047357.01.T01

ATGATGAATGGAAGAAACAGGTATCCTTTTACTCCAACTCAGTGGCAAGAGCTTGAACATCAAGCTCTTATCTACAAATACATGGCTTCTGGTATTTCCATTCCTCCTGATCTTCTACTCACTATCAAAAGAAGCTACTTAGACTCTTCAAGACTCTCACCTCATCAGTCTCAACACTTTGGATGGAACTATTTACCAATGGGATTGGGAAGAAAAATAGACCCAGAGCCAGGGAGGTGCAGAAGAACAGATGGAAAGAAATGGAGGTGTTCAAAAGAAGCATATCCAGATTCAAAGTATGTTTATGGACTGAAAGAGGAGGTGGATGAACATGCTTTCTTCACTGAACCTTCTGGAACTATAAGAAGTTTATCAGCTTCTTCAATGGATGATTCATGGCAACTCACACCACTTACCATAAGCTCCTCTTCCTCTTTTAAACAGAGAAATTGTTCTGGTTTATCCAATGACAATGAGTACTCTTACTTGCAACTTGACAACAACTCAAAACAACCACAGCAAGATCAAGGTTGTTACATCTCAGGTAGTGAAGAACATTCATGA

Protein sequence

>MsG0880047357.01.T01

MMNGRNRYPFTPTQWQELEHQALIYKYMASGISIPPDLLLTIKRSYLDSSRLSPHQSQHFGWNYLPMGLGRKIDPEPGRCRRTDGKKWRCSKEAYPDSKYVYGLKEEVDEHAFFTEPSGTIRSLSASSMDDSWQLTPLTISSSSSFKQRNCSGLSNDNEYSYLQLDNNSKQPQQDQGCYISGSEEHS*