AlfalfaGEDB | Msa0024610

M. sativa cultivar zhongmu-4 / Msa0024610

KEGG: K15200
GO: -
PFAM: -

Nr:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa0024610	XP_013468026.1	97.068	648	19	0	1	648	1	648	0.0	1308

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Swissprot:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
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Trembl:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
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TFs/TRs:

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Gene ID	Type	Classification
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Protein Kinases:

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Network:

Co-expression Network

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Gene1	Gene2	correlation coefficient	p_value	FDR
Msa0024610	Msa0163720	0.805740	1.153588e-49	-8.615850e-47
Msa0024610	Msa0355170	0.848439	6.123370e-60	-8.615850e-47
Msa0024610	Msa0355180	0.845970	2.913278e-59	-8.615850e-47
Msa0024610	Msa0355190	0.868495	6.258247e-66	-8.615850e-47
Msa0024610	Msa0402240	0.848615	5.476683e-60	-8.615850e-47
Msa0024610	Msa0402250	0.855693	5.316949e-62	-8.615850e-47
Msa0024610	Msa0708900	0.853822	1.853691e-61	-8.615850e-47
Msa0024610	Msa0708910	0.838363	3.007221e-57	-8.615850e-47

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PPI

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Gene1	Gene2	Type
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Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa0024610	MtrunA17_Chr1g0178981	97.068	648	19	0	1	648	1	648	0.0	1308

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Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa0024610	AT1G19485.1	40.433	601	242	19	57	647	3	497	1.84e-119	375
Msa0024610	AT1G19485.2	40.433	601	242	19	57	647	3	497	1.84e-119	375

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Find 164 sgRNAs with CRISPR-Local

Find 45 sgRNAs with CRISPR-GE

CRISPR-Local

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CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
AAAAGCATTAGGTTTACTTA+CGG	0.463447	1_1:-47252797	Msa0024610:CDS
AAAATGGGTGCCTCACTTAC+TGG	0.325896	1_1:-47250832	Msa0024610:CDS
AAACAAGAGAGTGATGGAAG+AGG	0.590776	1_1:-47253701	Msa0024610:exon
AAACCATACTTGGATTCTTG+AGG	0.544459	1_1:+47252500	None:intergenic
AAACTCTTGTTGCAAGAAGT+AGG	0.524383	1_1:-47250168	Msa0024610:CDS
AAAGCTGCTCCGAATACAAA+GGG	0.553544	1_1:-47250229	Msa0024610:CDS
AAAGCTGCTGCGAATACAAA+GGG	0.552131	1_1:-47250265	Msa0024610:CDS
AAATAAAGAGGCCTAGAGGA+AGG	0.580422	1_1:-47250684	Msa0024610:CDS
AAATGCCCTTTACTTTACCT+AGG	0.608444	1_1:+47253832	None:intergenic
AAATGCTCAATGTTGAAACG+TGG	0.713740	1_1:-47248514	Msa0024610:CDS

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CRISPR-GE

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sgRNA_sequence	Strand	Position	Region	GC_content
GGAAGTTCAAAACAAACTAT+AGG	-	chr1_1:47253202-47253221	Msa0024610:intron	30.0%
TGTTCTTACGTTTATCTAGT+TGG	-	chr1_1:47253665-47253684	Msa0024610:CDS	30.0%
GTTCTTACGTTTATCTAGTT+GGG	-	chr1_1:47253666-47253685	Msa0024610:CDS	30.0%
TTCTTACGTTTATCTAGTTG+GGG	-	chr1_1:47253667-47253686	Msa0024610:CDS	30.0%
ATTATCACTAACCCTTCAAA+AGG	+	chr1_1:47253843-47253862	None:intergenic	30.0%
ATAGGCCAATCATTTAAGAG+AGG	-	chr1_1:47253220-47253239	Msa0024610:intron	35.0%
CCAAACCTCTCTTAAATGAT+TGG	+	chr1_1:47253228-47253247	None:intergenic	35.0%
CCAATCATTTAAGAGAGGTT+TGG	-	chr1_1:47253225-47253244	Msa0024610:intron	35.0%
CAATACCTGACAGCATATTA+TGG	-	chr1_1:47253272-47253291	Msa0024610:intron	35.0%
TTTATATGTAGGTTGCTCTG+TGG	-	chr1_1:47253586-47253605	Msa0024610:CDS	35.0%

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Chromosome	Type	Strat	End	Strand	Name
chr1_1	gene	47246112	47253889	47246112	ID=Msa0024610;Name=Msa0024610
chr1_1	mRNA	47246112	47253889	47246112	ID=Msa0024610-mRNA-1;Parent=Msa0024610;Name=Msa0024610-mRNA-1;_AED=0.15;_eAED=0.15;_QI=179\|0.87\|0.88\|1\|1\|1\|9\|23\|652
chr1_1	exon	47246112	47246223	47246112	ID=Msa0024610-mRNA-1:exon:13458;Parent=Msa0024610-mRNA-1
chr1_1	exon	47246359	47246404	47246359	ID=Msa0024610-mRNA-1:exon:13457;Parent=Msa0024610-mRNA-1
chr1_1	exon	47247448	47247520	47247448	ID=Msa0024610-mRNA-1:exon:13456;Parent=Msa0024610-mRNA-1
chr1_1	exon	47248502	47248627	47248502	ID=Msa0024610-mRNA-1:exon:13455;Parent=Msa0024610-mRNA-1
chr1_1	exon	47249875	47250896	47249875	ID=Msa0024610-mRNA-1:exon:13454;Parent=Msa0024610-mRNA-1
chr1_1	exon	47252246	47252336	47252246	ID=Msa0024610-mRNA-1:exon:13453;Parent=Msa0024610-mRNA-1
chr1_1	exon	47252509	47252561	47252509	ID=Msa0024610-mRNA-1:exon:13452;Parent=Msa0024610-mRNA-1
chr1_1	exon	47252712	47252847	47252712	ID=Msa0024610-mRNA-1:exon:13451;Parent=Msa0024610-mRNA-1
chr1_1	exon	47253388	47253889	47253388	ID=Msa0024610-mRNA-1:exon:13450;Parent=Msa0024610-mRNA-1
chr1_1	five_prime_UTR	47253711	47253889	47253711	ID=Msa0024610-mRNA-1:five_prime_utr;Parent=Msa0024610-mRNA-1
chr1_1	CDS	47253388	47253710	47253388	ID=Msa0024610-mRNA-1:cds;Parent=Msa0024610-mRNA-1
chr1_1	CDS	47252712	47252847	47252712	ID=Msa0024610-mRNA-1:cds;Parent=Msa0024610-mRNA-1
chr1_1	CDS	47252509	47252561	47252509	ID=Msa0024610-mRNA-1:cds;Parent=Msa0024610-mRNA-1
chr1_1	CDS	47252246	47252336	47252246	ID=Msa0024610-mRNA-1:cds;Parent=Msa0024610-mRNA-1
chr1_1	CDS	47249875	47250896	47249875	ID=Msa0024610-mRNA-1:cds;Parent=Msa0024610-mRNA-1
chr1_1	CDS	47248502	47248627	47248502	ID=Msa0024610-mRNA-1:cds;Parent=Msa0024610-mRNA-1
chr1_1	CDS	47247448	47247520	47247448	ID=Msa0024610-mRNA-1:cds;Parent=Msa0024610-mRNA-1
chr1_1	CDS	47246359	47246404	47246359	ID=Msa0024610-mRNA-1:cds;Parent=Msa0024610-mRNA-1
chr1_1	CDS	47246135	47246223	47246135	ID=Msa0024610-mRNA-1:cds;Parent=Msa0024610-mRNA-1
chr1_1	three_prime_UTR	47246112	47246134	47246112	ID=Msa0024610-mRNA-1:three_prime_utr;Parent=Msa0024610-mRNA-1

Gene Sequence

>Msa0024610

ATGGAAGAGGAAAATGAGAACCTAACTATTGCCACACTATTTAATATCAAAGAGAAAAAGAAGAAGAAAAAGAAGAAAAAGACGAAGGCAAATAAAGAGAGCGAAAGAGTTGCTGATGATTCTGCTACAGCGGCAAATGATGAAGGTGGTGGTGGTAGTACCAGCAGTGGTGCTAACCCTAAGGTTTCTTTGTTTGATATCTCTGTCGAGAATTTCTTTGAAGACATGGACACCATTGCCAAACTCTGTGGGGAAGAAGAACGCAACACTGCTGTTGAGCAAAGCGAGATCAAGAGAATGTTTTCTTCCGTCACTTTTTTAAGAGAATGGAAGGATTTTAAATATCCATCTAAAAGCATTAGGTTTACTTACGGAATTGGGAGCTCTGAGTGTTATGAGAGAAATGATATCAAAGATGACATAAACTTACCTCAGTTTTCATCTGCCACTGTTCCTAAGCATAATATGCAAAAGGAGGAGCAACTTGGGGATGCCAAACCTCAAGAATCCAAAGACTTTGTGATGAATGTTGGAGGTTCTGTATGGGCATTAGATTGGTGTCCTAGGATGCATGGGGAGCCTGATTGTTCAATTAAATGTGAGTTCATTGCTGTTGCTGCTCATCCGCCTGGTTCATCTTATCACAAAATGGGTGCCTCACTTACTGGCAGAGGTGCTGTGCAAATATGGTGTCTTCTAAATATCAGGGAACACAGCGAAGAAGTATCATATCTCCCCGGGAAGAAGGAAAAAAAACCTAAAAAAGACACGTGCACAAATGACAAATCGATAGAAATAAAGAGGCCTAGAGGAAGGCCTAGAAAGAATCCTACTGAAAACAACGAAGCAATATCTCCTATTACAAACAAGAGAAAAAGAGGAAGACCAAAAAAGAATCCAACTGTAATAGCAGTGGATAGTACAAATTGTGGGACCAAGTACATAGCATCTGGTAGTGTCTCAAATGGGAATAATGAAAACAATGAAGAAATACTTCGTATTACATACAAGAGTAAAAAAAGGCCTAAAGGAAGACCGAAAAAGAATTCAAAAGATGGAACAGTGAGTGATCCAAATTGTGAGAACCAATTTGTTCCTCTTACTGTTCAACTTCCAGATTCAGCTGAATTTATTTCTCCAGATGTAGTTCCTGGCAGTTCTGATGAACATCATTCACAACAATTTTCTAATACAAAGGGAAAGAATGCCAAGAAAGCTGCTGCGAATACAAAGGGAAAGAAGGCTAAGAAAGCTGCTCCGAATACAAAGGGAAAGAATGCCAAGAAAGCTGCTTCTGCATACGATTCAGAAACTCTTGTTGCAAGAAGTAGGTTGGATATTAATCATAGGGAAAGAAGTTGTAGCCCAGATACAAGTAGGCCATTATTGATTCAGTGTGAGAATGAGGCAAATCATCAACCACATGGTAGCCCTGTATTGGAACCTCAAGCATCTACTTGTCCCATTCCACAAAATGTAGCATTGCCTAGAGTTGTGTCATGCCTAGCTCATAATGGAAAGGTGGCATGGGATGTCAAATGGAGGCCTCTTAGCAATCTTGATTCCTCATGCAAGCATCGAATGGGCTATCTTGCTGTCTTGTTGGGCAATGGATCTCTGGAAGTGTGGGAGGTTCCTCTTCCTCATGCACTGAGAGCAGTTTATACGCAGAAGGAGGGCACAGATCCTCGTTTTATAAAATTGGAGCCTGTATTCAAATGCTCAATGTTGAAACGTGGCAGCTTACAGAGCATTCCACTGACAGTGGAGTGGTCAGTTACTCCCCCTCATGATTATTTACTTGCTGGCTGCCATGATGGAACGGTTGCTCTGTGGAAATTCTCTACAAATTCTTCATCTAAATGTGATGATACAAAGCCTATACTTTGTTTCGGTGGGGATACTGTTCCTATTAGAACAGTTGCATGGGCTCCTTTTGAAGGGTTAGTGATAATTTAA

Protein sequence

>Msa0024610

MEEENENLTIATLFNIKEKKKKKKKKKTKANKESERVADDSATAANDEGGGGSTSSGANPKVSLFDISVENFFEDMDTIAKLCGEEERNTAVEQSEIKRMFSSVTFLREWKDFKYPSKSIRFTYGIGSSECYERNDIKDDINLPQFSSATVPKHNMQKEEQLGDAKPQESKDFVMNVGGSVWALDWCPRMHGEPDCSIKCEFIAVAAHPPGSSYHKMGASLTGRGAVQIWCLLNIREHSEEVSYLPGKKEKKPKKDTCTNDKSIEIKRPRGRPRKNPTENNEAISPITNKRKRGRPKKNPTVIAVDSTNCGTKYIASGSVSNGNNENNEEILRITYKSKKRPKGRPKKNSKDGTVSDPNCENQFVPLTVQLPDSAEFISPDVVPGSSDEHHSQQFSNTKGKNAKKAAANTKGKKAKKAAPNTKGKNAKKAASAYDSETLVARSRLDINHRERSCSPDTSRPLLIQCENEANHQPHGSPVLEPQASTCPIPQNVALPRVVSCLAHNGKVAWDVKWRPLSNLDSSCKHRMGYLAVLLGNGSLEVWEVPLPHALRAVYTQKEGTDPRFIKLEPVFKCSMLKRGSLQSIPLTVEWSVTPPHDYLLAGCHDGTVALWKFSTNSSSKCDDTKPILCFGGDTVPIRTVAWAPFEGLVII*