Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
| Gene ID | Type | Classification |
|---|
| Gene ID | Type | Classification |
|---|
Co-expression Network
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| Msa0063040 | Msa0213670 | 0.834609 | 2.714874e-56 | -8.615850e-47 |
| Msa0063040 | Msa0319860 | 0.839046 | 2.002436e-57 | -8.615850e-47 |
| Msa0063040 | Msa0719080 | 0.810397 | 1.176590e-50 | -8.615850e-47 |
| Msa0063040 | Msa0719090 | 0.809192 | 2.136489e-50 | -8.615850e-47 |
| Msa0063040 | Msa0719390 | 0.802339 | 5.880193e-49 | -8.615850e-47 |
| Msa0063040 | Msa0789640 | 0.805511 | 1.288706e-49 | -8.615850e-47 |
| Msa0063040 | Msa0872150 | 0.825437 | 4.690547e-54 | -8.615850e-47 |
| Msa0063040 | Msa1305900 | 0.804467 | 2.130403e-49 | -8.615850e-47 |
| Msa0063040 | Msa1338620 | 0.882455 | 1.027251e-70 | -8.615850e-47 |
PPI
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
Find 20 sgRNAs with CRISPR-Local
Find 114 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| AAGAAAGTTATGAGCCTTTC+CGG | 0.210967 | 1_2:+23184497 | None:intergenic |
| TCTAACAATGTATTATTTAT+AGG | 0.218742 | 1_2:-23184526 | Msa0063040:three_prime_UTR |
| CAATGGGATATGGTTGAGTT+TGG | 0.233721 | 1_2:-23185708 | Msa0063040:CDS |
| TCTGCTGAGTTCTAAATTCT+TGG | 0.236360 | 1_2:-23184368 | Msa0063040:three_prime_UTR |
| TATGGTTGAGTTTGGGGATC+TGG | 0.276601 | 1_2:-23185700 | Msa0063040:CDS |
| TTAGAACTCAGCAGATTTGA+TGG | 0.337391 | 1_2:+23184376 | None:intergenic |
| TGTTTGTTAGCAATGGGATA+TGG | 0.369203 | 1_2:-23185718 | Msa0063040:intron |
| AATGGGATATGGTTGAGTTT+GGG | 0.371906 | 1_2:-23185707 | Msa0063040:CDS |
| TTGCTTGAGTGATATAAGAT+TGG | 0.398556 | 1_2:+23184608 | None:intergenic |
| AGAAAGTTATGAGCCTTTCC+GGG | 0.437598 | 1_2:+23184498 | None:intergenic |
| TTTATAGGAGTTACCCGGAA+AGG | 0.527417 | 1_2:-23184511 | Msa0063040:three_prime_UTR |
| TATTACAATCTGCACTGTGC+AGG | 0.530753 | 1_2:+23184406 | None:intergenic |
| TAGCACCACTTACAAAATCA+AGG | 0.542360 | 1_2:-23186876 | Msa0063040:intron |
| TGCAGGCTATCTCTTGATAG+AGG | 0.543045 | 1_2:+23184423 | None:intergenic |
| ATGGGATATGGTTGAGTTTG+GGG | 0.560484 | 1_2:-23185706 | Msa0063040:CDS |
| TATTATTTATAGGAGTTACC+CGG | 0.601233 | 1_2:-23184516 | Msa0063040:three_prime_UTR |
| ATAAGATTGGAAAACATGCG+TGG | 0.659962 | 1_2:+23184621 | None:intergenic |
| ACTAAAGTAGATAAGAACAA+TGG | 0.664356 | 1_2:-23184560 | Msa0063040:three_prime_UTR |
| GAAGAAGCAATAGTTCAGCA+AGG | 0.666882 | 1_2:-23185678 | Msa0063040:CDS |
| AGATTGGAAAACATGCGTGG+AGG | 0.724170 | 1_2:+23184624 | None:intergenic |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !! | ATATAATTTACTTTATAAGA+AGG | - | chr1_2:23185916-23185935 | Msa0063040:intron | 10.0% |
| !! | TATAATTTACTTTATAAGAA+GGG | - | chr1_2:23185917-23185936 | Msa0063040:intron | 10.0% |
| !!! | AACAAAAATTTTGATAGAAT+TGG | + | chr1_2:23184426-23184445 | None:intergenic | 15.0% |
| !! | TTATTTCATATTCAAAAGTT+TGG | - | chr1_2:23184724-23184743 | Msa0063040:intron | 15.0% |
| !! | AAGAATGAACTTAATTAAAA+TGG | + | chr1_2:23185113-23185132 | None:intergenic | 15.0% |
| !! | AAAACAATATTCACAAAATA+AGG | + | chr1_2:23185397-23185416 | None:intergenic | 15.0% |
| !! | TCTAACAATGTATTATTTAT+AGG | - | chr1_2:23186681-23186700 | Msa0063040:intron | 15.0% |
| !! | ATCTTTAATGGAAATATGAT+TGG | + | chr1_2:23184511-23184530 | None:intergenic | 20.0% |
| !! | TAGAACTTATAAAGAACTTA+AGG | + | chr1_2:23184792-23184811 | None:intergenic | 20.0% |
| !! | ATGAACTTAATTAAAATGGA+GGG | + | chr1_2:23185109-23185128 | None:intergenic | 20.0% |
| !! | AATGAACTTAATTAAAATGG+AGG | + | chr1_2:23185110-23185129 | None:intergenic | 20.0% |
| !! | AATATGTTGAAATAATGCAT+AGG | - | chr1_2:23185678-23185697 | Msa0063040:CDS | 20.0% |
| !! | ATATGTTGAAATAATGCATA+GGG | - | chr1_2:23185679-23185698 | Msa0063040:CDS | 20.0% |
| !! | TAAGTTATAACATCTCAATA+AGG | + | chr1_2:23185874-23185893 | None:intergenic | 20.0% |
| !! | AATGAATACCATAAAATGAT+GGG | - | chr1_2:23185974-23185993 | Msa0063040:intron | 20.0% |
| !!! | TTTTAGCTTTAGTTATCATT+TGG | - | chr1_2:23186022-23186041 | Msa0063040:intron | 20.0% |
| !!! | AGCTTTAAACTTAATTTTTG+TGG | - | chr1_2:23186141-23186160 | Msa0063040:intron | 20.0% |
| !! | CATTAAATCATACATAAAGT+TGG | + | chr1_2:23186219-23186238 | None:intergenic | 20.0% |
| !! | GAAAAATAAATGTAAGAAAC+AGG | + | chr1_2:23186353-23186372 | None:intergenic | 20.0% |
| !! | AATCCACTACAATTAAAAAA+TGG | - | chr1_2:23186479-23186498 | Msa0063040:intron | 20.0% |
| !!! | CAACAATGTATATATGTTTT+AGG | - | chr1_2:23186515-23186534 | Msa0063040:intron | 20.0% |
| !!! | CATTGTTTTTTTTTTTTTGC+AGG | - | chr1_2:23186553-23186572 | Msa0063040:intron | 20.0% |
| !! | TATTACCTTGATTTTGTAAG+TGG | + | chr1_2:23184339-23184358 | None:intergenic | 25.0% |
| ! | CATAAGATAATGCAAAAAAG+AGG | - | chr1_2:23184397-23184416 | Msa0063040:three_prime_UTR | 25.0% |
| !! | AAAGTTTGGTAATATCTGTT+AGG | - | chr1_2:23184738-23184757 | Msa0063040:intron | 25.0% |
| !! | AAGTTTGGTAATATCTGTTA+GGG | - | chr1_2:23184739-23184758 | Msa0063040:intron | 25.0% |
| ! | CATACTCATAGACTAATTTA+TGG | - | chr1_2:23184839-23184858 | Msa0063040:intron | 25.0% |
| ! | TTGAGAGTTTCTTCTAAATT+AGG | - | chr1_2:23184926-23184945 | Msa0063040:intron | 25.0% |
| !! | GGAACTTTTTATATTAGTGT+TGG | + | chr1_2:23185255-23185274 | None:intergenic | 25.0% |
| !!! | TTTTCTTTGTTTGTTAGCAA+TGG | - | chr1_2:23185482-23185501 | Msa0063040:intron | 25.0% |
| !! | TTTCTTTGTTTGTTAGCAAT+GGG | - | chr1_2:23185483-23185502 | Msa0063040:intron | 25.0% |
| !!! | AAAGAGGTATTAGATAGATA+AGG | + | chr1_2:23185597-23185616 | None:intergenic | 25.0% |
| ! | TTATCTAACAACAGAAAAAG+AGG | + | chr1_2:23185613-23185632 | None:intergenic | 25.0% |
| ! | TATGTTGAAATAATGCATAG+GGG | - | chr1_2:23185680-23185699 | Msa0063040:CDS | 25.0% |
| ! | ATATGAAAAAAGACCTTCAT+AGG | + | chr1_2:23185717-23185736 | None:intergenic | 25.0% |
| ! | CAATGAATACCATAAAATGA+TGG | - | chr1_2:23185973-23185992 | Msa0063040:intron | 25.0% |
| ! | AATAAATTATCATGCAAAGC+TGG | + | chr1_2:23186058-23186077 | None:intergenic | 25.0% |
| ! | TACCATAAAATGATGTATAG+CGG | - | chr1_2:23186241-23186260 | Msa0063040:intron | 25.0% |
| !!! | CTTACTTTTCACATATACAA+AGG | + | chr1_2:23186281-23186300 | None:intergenic | 25.0% |
| !!! | CATCCATTTTTTAATTGTAG+TGG | + | chr1_2:23186485-23186504 | None:intergenic | 25.0% |
| ! | CACTACAATTAAAAAATGGA+TGG | - | chr1_2:23186483-23186502 | Msa0063040:intron | 25.0% |
| ! | ACTAAAGTAGATAAGAACAA+TGG | - | chr1_2:23186647-23186666 | Msa0063040:intron | 25.0% |
| ! | TATTATTTATAGGAGTTACC+CGG | - | chr1_2:23186691-23186710 | Msa0063040:intron | 25.0% |
| AACGAGTAAATATGGTATGT+TGG | + | chr1_2:23184370-23184389 | None:intergenic | 30.0% | |
| GCATTGAAAACGAGTAAATA+TGG | + | chr1_2:23184378-23184397 | None:intergenic | 30.0% | |
| ! | TTTGTTGTATATTCACATGC+TGG | - | chr1_2:23184440-23184459 | Msa0063040:three_prime_UTR | 30.0% |
| ! | ACTTGCGTTTTCATCTTTAA+TGG | + | chr1_2:23184523-23184542 | None:intergenic | 30.0% |
| !! | ATCTAAACTTGTTAGAGTGT+AGG | - | chr1_2:23184624-23184643 | Msa0063040:three_prime_UTR | 30.0% |
| !! | TCTAAACTTGTTAGAGTGTA+GGG | - | chr1_2:23184625-23184644 | Msa0063040:three_prime_UTR | 30.0% |
| ! | ATTAGTCTATGAGTATGACT+GGG | + | chr1_2:23184836-23184855 | None:intergenic | 30.0% |
| ! | AATTAGTCTATGAGTATGAC+TGG | + | chr1_2:23184837-23184856 | None:intergenic | 30.0% |
| ! | ATGTAAGCATTCCTTTTAAG+AGG | + | chr1_2:23184873-23184892 | None:intergenic | 30.0% |
| CACATCTATTAGAAATGGTT+AGG | + | chr1_2:23185168-23185187 | None:intergenic | 30.0% | |
| ACCATTTCTAATAGATGTGA+TGG | - | chr1_2:23185169-23185188 | Msa0063040:intron | 30.0% | |
| CCATTTCTAATAGATGTGAT+GGG | - | chr1_2:23185170-23185189 | Msa0063040:intron | 30.0% | |
| CATTTCTAATAGATGTGATG+GGG | - | chr1_2:23185171-23185190 | Msa0063040:intron | 30.0% | |
| !! | GCAAAAGTCTTATTAGCATA+GGG | - | chr1_2:23185193-23185212 | Msa0063040:intron | 30.0% |
| !! | CAAAAGTCTTATTAGCATAG+GGG | - | chr1_2:23185194-23185213 | Msa0063040:intron | 30.0% |
| ACTATACATCAAGCTTGATA+AGG | + | chr1_2:23185355-23185374 | None:intergenic | 30.0% | |
| GATAGATAAGGAAAACTTAC+AGG | + | chr1_2:23185585-23185604 | None:intergenic | 30.0% | |
| AACCTCAATGCATTTAGAAA+GGG | + | chr1_2:23185646-23185665 | None:intergenic | 30.0% | |
| AAAAAGACCTTCATAGGAAA+AGG | + | chr1_2:23185711-23185730 | None:intergenic | 30.0% | |
| AAATTTCTCAGTTGTATAGC+AGG | + | chr1_2:23185741-23185760 | None:intergenic | 30.0% | |
| ! | CTTTTTGACCCATCATTTTA+TGG | + | chr1_2:23185985-23186004 | None:intergenic | 30.0% |
| ACCATAAAATGATGTATAGC+GGG | - | chr1_2:23186242-23186261 | Msa0063040:intron | 30.0% | |
| TGAAAAGTAAGAGAATGAAG+AGG | - | chr1_2:23186290-23186309 | Msa0063040:intron | 30.0% | |
| TAGTAGAGTTATTACAAGAG+AGG | + | chr1_2:23186389-23186408 | None:intergenic | 30.0% | |
| TTGCTTGAGTGATATAAGAT+TGG | + | chr1_2:23186602-23186621 | None:intergenic | 30.0% | |
| TAGCACCACTTACAAAATCA+AGG | - | chr1_2:23184331-23184350 | Msa0063040:three_prime_UTR | 35.0% | |
| TGGTAGTTTAGCCTCTTAAA+AGG | - | chr1_2:23184859-23184878 | Msa0063040:intron | 35.0% | |
| !!! | CTGATTGCTCTTGTTTTTTG+TGG | - | chr1_2:23184993-23185012 | Msa0063040:intron | 35.0% |
| CCCATCACATCTATTAGAAA+TGG | + | chr1_2:23185173-23185192 | None:intergenic | 35.0% | |
| !! | GGCAAAAGTCTTATTAGCAT+AGG | - | chr1_2:23185192-23185211 | Msa0063040:intron | 35.0% |
| TCCTGTGACAATTTGCATAA+GGG | + | chr1_2:23185323-23185342 | None:intergenic | 35.0% | |
| ATCCTGTGACAATTTGCATA+AGG | + | chr1_2:23185324-23185343 | None:intergenic | 35.0% | |
| ATGCAAATTGTCACAGGATA+AGG | - | chr1_2:23185325-23185344 | Msa0063040:intron | 35.0% | |
| ! | TGTTTGTTAGCAATGGGATA+TGG | - | chr1_2:23185489-23185508 | Msa0063040:intron | 35.0% |
| AATGGGATATGGTTGAGTTT+GGG | - | chr1_2:23185500-23185519 | Msa0063040:intron | 35.0% | |
| TCAATGCATTTAGAAAGGGT+TGG | + | chr1_2:23185642-23185661 | None:intergenic | 35.0% | |
| AACCCTTTCTAAATGCATTG+AGG | - | chr1_2:23185641-23185660 | Msa0063040:intron | 35.0% | |
| CAACCTCAATGCATTTAGAA+AGG | + | chr1_2:23185647-23185666 | None:intergenic | 35.0% | |
| ! | TCCCGCTATACATCATTTTA+TGG | + | chr1_2:23186246-23186265 | None:intergenic | 35.0% |
| GAGGTTAAACTGTGTATAGT+TGG | - | chr1_2:23186309-23186328 | Msa0063040:intron | 35.0% | |
| TAGAGTTATTACAAGAGAGG+TGG | + | chr1_2:23186386-23186405 | None:intergenic | 35.0% | |
| ! | GAGTATTGTGAAGAAAGAGT+GGG | + | chr1_2:23186418-23186437 | None:intergenic | 35.0% |
| !! | AGAGTATTGTGAAGAAAGAG+TGG | + | chr1_2:23186419-23186438 | None:intergenic | 35.0% |
| ATAAGATTGGAAAACATGCG+TGG | + | chr1_2:23186589-23186608 | None:intergenic | 35.0% | |
| AAGAAAGTTATGAGCCTTTC+CGG | + | chr1_2:23186713-23186732 | None:intergenic | 35.0% | |
| TTAGAACTCAGCAGATTTGA+TGG | + | chr1_2:23186834-23186853 | None:intergenic | 35.0% | |
| !! | TCTGCTGAGTTCTAAATTCT+TGG | - | chr1_2:23186839-23186858 | Msa0063040:intron | 35.0% |
| GTCTCTCATCATAACTCAGT+AGG | + | chr1_2:23185046-23185065 | None:intergenic | 40.0% | |
| TACAGTAGGTGAATCTAGGT+GGG | + | chr1_2:23185079-23185098 | None:intergenic | 40.0% | |
| AACGTACAGTAGGTGAATCT+AGG | + | chr1_2:23185083-23185102 | None:intergenic | 40.0% | |
| ACCCTTATGCAAATTGTCAC+AGG | - | chr1_2:23185319-23185338 | Msa0063040:intron | 40.0% | |
| CAATGGGATATGGTTGAGTT+TGG | - | chr1_2:23185499-23185518 | Msa0063040:intron | 40.0% | |
| ATGGGATATGGTTGAGTTTG+GGG | - | chr1_2:23185501-23185520 | Msa0063040:intron | 40.0% | |
| GAAGAAGCAATAGTTCAGCA+AGG | - | chr1_2:23185529-23185548 | Msa0063040:intron | 40.0% | |
| ! | GGTTATGCCTTTTCCTATGA+AGG | - | chr1_2:23185701-23185720 | Msa0063040:CDS | 40.0% |
| CTTTCTTCACAATACTCTGC+AGG | - | chr1_2:23186421-23186440 | Msa0063040:intron | 40.0% | |
| TTTATAGGAGTTACCCGGAA+AGG | - | chr1_2:23186696-23186715 | Msa0063040:intron | 40.0% | |
| AGAAAGTTATGAGCCTTTCC+GGG | + | chr1_2:23186712-23186731 | None:intergenic | 40.0% | |
| TATTACAATCTGCACTGTGC+AGG | + | chr1_2:23186804-23186823 | None:intergenic | 40.0% | |
| ACAGTAGGTGAATCTAGGTG+GGG | + | chr1_2:23185078-23185097 | None:intergenic | 45.0% | |
| GTACAGTAGGTGAATCTAGG+TGG | + | chr1_2:23185080-23185099 | None:intergenic | 45.0% | |
| TGGAGGGAAAAACGTACAGT+AGG | + | chr1_2:23185093-23185112 | None:intergenic | 45.0% | |
| TATGGTTGAGTTTGGGGATC+TGG | - | chr1_2:23185507-23185526 | Msa0063040:intron | 45.0% | |
| AGATTGGAAAACATGCGTGG+AGG | + | chr1_2:23186586-23186605 | None:intergenic | 45.0% | |
| TGCAGGCTATCTCTTGATAG+AGG | + | chr1_2:23186787-23186806 | None:intergenic | 45.0% | |
| !!! | TAGAAATATTATTTAAAATT+AGG | + | chr1_2:23185276-23185295 | None:intergenic | 5.0% |
| !!! | TTTAAATAATATTTCTAATT+AGG | - | chr1_2:23185279-23185298 | Msa0063040:intron | 5.0% |
| TGAATCTAGGTGGGGGAAGT+GGG | + | chr1_2:23185070-23185089 | None:intergenic | 50.0% | |
| CAGTAGGTGAATCTAGGTGG+GGG | + | chr1_2:23185077-23185096 | None:intergenic | 50.0% | |
| AACTCAGTAGGTCGCTGCTC+TGG | + | chr1_2:23185034-23185053 | None:intergenic | 55.0% | |
| GTGAATCTAGGTGGGGGAAG+TGG | + | chr1_2:23185071-23185090 | None:intergenic | 55.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr1_2 | gene | 23184323 | 23186906 | 23184323 | ID=Msa0063040;Name=Msa0063040 |
| chr1_2 | mRNA | 23184323 | 23186906 | 23184323 | ID=Msa0063040-mRNA-1;Parent=Msa0063040;Name=Msa0063040-mRNA-1;_AED=0.30;_eAED=0.30;_QI=0|0.5|0.66|0.66|0.5|0.66|3|325|40 |
| chr1_2 | exon | 23184323 | 23184654 | 23184323 | ID=Msa0063040-mRNA-1:exon:8482;Parent=Msa0063040-mRNA-1 |
| chr1_2 | exon | 23185645 | 23185730 | 23185645 | ID=Msa0063040-mRNA-1:exon:8481;Parent=Msa0063040-mRNA-1 |
| chr1_2 | exon | 23186877 | 23186906 | 23186877 | ID=Msa0063040-mRNA-1:exon:8480;Parent=Msa0063040-mRNA-1 |
| chr1_2 | CDS | 23186877 | 23186906 | 23186877 | ID=Msa0063040-mRNA-1:cds;Parent=Msa0063040-mRNA-1 |
| chr1_2 | CDS | 23185645 | 23185730 | 23185645 | ID=Msa0063040-mRNA-1:cds;Parent=Msa0063040-mRNA-1 |
| chr1_2 | CDS | 23184648 | 23184654 | 23184648 | ID=Msa0063040-mRNA-1:cds;Parent=Msa0063040-mRNA-1 |
| chr1_2 | three_prime_UTR | 23184323 | 23184647 | 23184323 | ID=Msa0063040-mRNA-1:three_prime_utr;Parent=Msa0063040-mRNA-1 |
| Gene Sequence |
| Protein sequence |
