AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa0063950

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0063950 MtrunA17_Chr1g0154681 99.603 252 1 0 1 252 1 252 0.0 521
Msa0063950 MtrunA17_Chr1g0162711 70.000 240 67 2 1 240 1 235 9.85e-123 349
Msa0063950 MtrunA17_Chr1g0149791 57.216 194 75 1 39 232 14 199 4.63e-81 242
Msa0063950 MtrunA17_Chr3g0137121 64.024 164 59 0 39 202 14 177 2.89e-80 240
Msa0063950 MtrunA17_Chr4g0055821 64.000 150 54 0 41 190 21 170 7.02e-74 224
Msa0063950 MtrunA17_Chr5g0399541 62.162 148 56 0 41 188 22 169 7.84e-72 219
Msa0063950 MtrunA17_Chr4g0002531 54.595 185 80 2 25 206 10 193 3.18e-69 213
Msa0063950 MtrunA17_Chr4g0063261 58.385 161 66 1 44 203 31 191 8.63e-68 209
Msa0063950 MtrunA17_Chr2g0306871 48.352 182 76 1 49 212 33 214 8.13e-65 201
Msa0063950 MtrunA17_Chr1g0202701 34.965 143 74 5 44 170 4 143 2.46e-18 81.3
Msa0063950 MtrunA17_Chr6g0468771 33.103 145 81 4 42 171 2 145 1.41e-17 79.7
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0063950 AT1G47740.1 71.875 256 63 4 1 252 29 279 7.08e-127 361
Msa0063950 AT1G47740.2 71.875 256 63 4 1 252 29 279 7.08e-127 361
Msa0063950 AT1G47740.3 71.875 256 63 4 1 252 29 279 7.08e-127 361
Msa0063950 AT5G25170.1 63.855 166 58 1 39 202 15 180 5.13e-77 232
Msa0063950 AT1G80690.1 56.044 182 79 1 28 209 2 182 4.97e-74 225
Msa0063950 AT2G25190.1 60.736 163 64 0 40 202 15 177 2.42e-73 224
Msa0063950 AT5G47310.1 64.828 145 51 0 42 186 28 172 9.30e-73 223
Msa0063950 AT1G80690.2 54.839 186 79 2 28 209 2 186 4.81e-72 220
Msa0063950 AT4G17486.1 58.974 156 64 0 33 188 17 172 1.35e-71 219
Msa0063950 AT4G31980.1 59.756 164 58 2 27 187 7 165 2.25e-63 210
Msa0063950 AT4G17486.2 60.145 138 55 0 33 170 17 154 2.17e-62 194
Msa0063950 AT5G25170.2 58.519 135 54 1 70 202 2 136 2.69e-53 170
Msa0063950 AT4G25680.1 32.639 144 87 4 28 169 8 143 4.06e-16 75.9
Msa0063950 AT4G25660.1 32.639 144 87 4 28 169 8 143 9.63e-16 75.1

Find 75 sgRNAs with CRISPR-Local

Find 186 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
ATGAAACATGCCAAGATTTA+AGG 0.115934 1_2:-24717352 None:intergenic
GATGATAAACAGCCTCTTAA+AGG 0.165224 1_2:-24720114 None:intergenic
GAAGAAATTCTTAGTGCTTC+TGG 0.179953 1_2:-24719961 None:intergenic
GTTTGTTATTGCGTAGTTTA+TGG 0.182070 1_2:+24717270 Msa0063950:five_prime_UTR
TTAGGGACATCCGATAAAAT+TGG 0.248199 1_2:-24720372 None:intergenic
ATTGTGCCTCTTCATTTAAA+AGG 0.274662 1_2:+24718567 Msa0063950:CDS
ACAATAACAATAGTCCCTTT+AGG 0.301623 1_2:-24720390 None:intergenic
GACATATGCTACAAGCTTAC+AGG 0.302536 1_2:+24719281 Msa0063950:CDS
CTTTGAATCCTGTTCAGGTT+AGG 0.313137 1_2:+24719177 Msa0063950:CDS
CTGTCTTTGTCTCTCTCTAA+TGG 0.319836 1_2:+24717408 Msa0063950:five_prime_UTR
TACATCTTTGAATCCTGTTC+AGG 0.336718 1_2:+24719172 Msa0063950:CDS
CTATCATGTGCCTCATGATT+AGG 0.345697 1_2:-24719997 None:intergenic
ATCTATTAACCGTTCCTTCA+AGG 0.354021 1_2:+24720164 Msa0063950:CDS
CAATTCCAACTTGGGTAAAT+CGG 0.354741 1_2:+24719309 Msa0063950:CDS
CAATAACAATAGTCCCTTTA+GGG 0.367363 1_2:-24720389 None:intergenic
TCATACACATTAAGATAAAC+TGG 0.373779 1_2:-24718660 None:intergenic
CCAATACACATAACCATTCA+TGG 0.376837 1_2:-24718691 None:intergenic
TGTTCAGGTTAGGGAGTTCA+TGG 0.381216 1_2:+24719187 Msa0063950:CDS
AAACAGTTACCTTTCCTTGA+AGG 0.382205 1_2:-24720178 None:intergenic
TTTGAATCCTGTTCAGGTTA+GGG 0.385241 1_2:+24719178 Msa0063950:CDS
TTTCTACAATCACCTTTAAG+AGG 0.386624 1_2:+24720102 Msa0063950:CDS
TGTTTCTTTGTACCTTGAAA+AGG 0.387538 1_2:+24720196 Msa0063950:three_prime_UTR
ATTATGTGAGTGTTCGCCTT+AGG 0.389160 1_2:+24718506 Msa0063950:five_prime_UTR
TTGTAGAAACAATGAAGATT+TGG 0.406339 1_2:-24720088 None:intergenic
CATGGTGTAGAATATGCATT+TGG 0.412888 1_2:+24719059 Msa0063950:CDS
CAGGGAAATCAATTCCAACT+TGG 0.414012 1_2:+24719300 Msa0063950:CDS
CATGAATGGTTATGTGTATT+GGG 0.415496 1_2:+24718692 Msa0063950:CDS
TATGTGTATTGGGCCGGTCT+TGG 0.423862 1_2:+24718702 Msa0063950:CDS
CCATGAATGGTTATGTGTAT+TGG 0.424620 1_2:+24718691 Msa0063950:CDS
ATGAACTCCCTAACCTGAAC+AGG 0.425276 1_2:-24719185 None:intergenic
CTAAACTAAACCTTAAATCT+TGG 0.426087 1_2:+24717342 Msa0063950:five_prime_UTR
GCAAGCCGATTTACCCAAGT+TGG 0.426198 1_2:-24719314 None:intergenic
AATGCCCAGGCTTCAAGTTC+AGG 0.427519 1_2:+24719129 Msa0063950:CDS
GTTTCTTTGTACCTTGAAAA+GGG 0.454647 1_2:+24720197 Msa0063950:three_prime_UTR
TTCAATTTCATCTTTCCCTA+AGG 0.460939 1_2:-24718522 None:intergenic
TTATGTGAGTGTTCGCCTTA+GGG 0.463750 1_2:+24718507 Msa0063950:five_prime_UTR
GACTTCCTGAACTTGAAGCC+TGG 0.467872 1_2:-24719134 None:intergenic
TATCATCAAAGTGGCCATCA+TGG 0.473681 1_2:+24720130 Msa0063950:CDS
GCACTAAGAATTTCTTCTGT+GGG 0.486160 1_2:+24719967 Msa0063950:CDS
TTATTGAGGTGTAACATTTG+CGG 0.489640 1_2:+24720446 Msa0063950:three_prime_UTR
AATTGCAAAATACTCCTCTC+TGG 0.498588 1_2:-24717227 None:intergenic
ATGAAATTGAAGTCTAAGAA+AGG 0.507796 1_2:+24718534 Msa0063950:CDS
ACTTCCTGAACTTGAAGCCT+GGG 0.521778 1_2:-24719133 None:intergenic
GATTTGCTCAATTCCCATGA+TGG 0.523695 1_2:-24720144 None:intergenic
AATTGAAGTCTAAGAAAGGA+TGG 0.524868 1_2:+24718538 Msa0063950:CDS
GTTGAACCTCGGCAATGCCC+AGG 0.525057 1_2:+24719116 Msa0063950:CDS
ATCTATCACTCTGGTGTCGA+AGG 0.531982 1_2:+24718726 Msa0063950:CDS
CGTAGTTTATGGTCTATGAC+AGG 0.531999 1_2:+24717281 Msa0063950:five_prime_UTR
ATCATCAAAGTGGCCATCAT+GGG 0.535412 1_2:+24720131 Msa0063950:CDS
TTAACCGTTCCTTCAAGGAA+AGG 0.547458 1_2:+24720169 Msa0063950:CDS
AGAGAGAGTGTTGCTCAAAA+CGG 0.548157 1_2:-24717387 None:intergenic
AGGGAAATCAATTCCAACTT+GGG 0.551036 1_2:+24719301 Msa0063950:CDS
GGTCTTGGTATCTATCACTC+TGG 0.557082 1_2:+24718717 Msa0063950:CDS
GTAAATCGGCTTGCTAAGCT+AGG 0.560765 1_2:+24719323 Msa0063950:CDS
TTCAGGAAGTCGATATTCAT+CGG 0.566691 1_2:+24719146 Msa0063950:CDS
CAATACACATAACCATTCAT+GGG 0.567668 1_2:-24718690 None:intergenic
GTTACCTTTCCTTGAAGGAA+CGG 0.569265 1_2:-24720173 None:intergenic
TCATTTAAAAGGAAAATCAC+CGG 0.579187 1_2:+24718578 Msa0063950:CDS
TTGAAGCCTGGGCATTGCCG+AGG 0.584621 1_2:-24719122 None:intergenic
AATTCAGCTAGCCATGGACC+TGG 0.589659 1_2:+24718630 Msa0063950:CDS
AATGGTTATGTGTATTGGGC+CGG 0.593181 1_2:+24718696 Msa0063950:CDS
ACTTACAAACAGACAAGAGA+TGG 0.594126 1_2:-24720292 None:intergenic
TATGACTTGACACCCATGAA+TGG 0.598526 1_2:+24718678 Msa0063950:CDS
ACATATGCTACAAGCTTACA+GGG 0.604015 1_2:+24719282 Msa0063950:CDS
AGCACTAAGAATTTCTTCTG+TGG 0.616876 1_2:+24719966 Msa0063950:CDS
GAGGCTGTTTATCATCAAAG+TGG 0.631803 1_2:+24720121 Msa0063950:CDS
CACTAAGAATTTCTTCTGTG+GGG 0.635774 1_2:+24719968 Msa0063950:CDS
AGAGTGATAGATACCAAGAC+CGG 0.635910 1_2:-24718715 None:intergenic
AAAGTAAATTCAGCTAGCCA+TGG 0.644369 1_2:+24718624 Msa0063950:CDS
AATAAGGGAGAGAGCCAGAG+AGG 0.645844 1_2:+24717213 None:intergenic
TATGGTCTATGACAGGACAA+TGG 0.650654 1_2:+24717288 Msa0063950:five_prime_UTR
TGTTGCTCAAAACGGTGAAG+TGG 0.655858 1_2:-24717379 None:intergenic
GGGGCATGATCCTAATCATG+AGG 0.671130 1_2:+24719987 Msa0063950:CDS
GCTCATGACTACCCAACAAG+CGG 0.680057 1_2:+24719083 Msa0063950:CDS
TGTGAGTGTGCAAACTACAA+CGG 0.683483 1_2:+24719212 Msa0063950:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!!! TTTTTATTTAATTATTTTAT+TGG + chr1_2:24720336-24720355 Msa0063950:three_prime_UTR 0.0%
!! TTAAAAATAAATAACTTACT+TGG - chr1_2:24718422-24718441 None:intergenic 10.0%
!! ATAATGTTAACATAATAAAT+AGG + chr1_2:24718448-24718467 Msa0063950:intron 10.0%
!!! TTCTACACAATTTTTTTATT+TGG + chr1_2:24717791-24717810 Msa0063950:intron 15.0%
!! AGTATAATAAGAATCTATTA+GGG + chr1_2:24717878-24717897 Msa0063950:intron 15.0%
!! AAACTAAAATTTCAACAATT+TGG - chr1_2:24718042-24718061 None:intergenic 15.0%
!! TTAATTAACAATATTAGCAT+TGG - chr1_2:24719762-24719781 None:intergenic 15.0%
!!! TTTTTGGATATTATTTATTG+AGG + chr1_2:24720432-24720451 Msa0063950:three_prime_UTR 15.0%
!! CATGAAAAAAAAATTGAGTT+AGG - chr1_2:24717715-24717734 None:intergenic 20.0%
!!! CTCAATTTTTTTTTCATGTT+TGG + chr1_2:24717717-24717736 Msa0063950:intron 20.0%
!! TGTTATAAACAAGAAATGAA+GGG - chr1_2:24717758-24717777 None:intergenic 20.0%
!! GTCAAAAAAAAAAAGAACAA+TGG - chr1_2:24717823-24717842 None:intergenic 20.0%
!! CAGTATAATAAGAATCTATT+AGG + chr1_2:24717877-24717896 Msa0063950:intron 20.0%
!!! TACAAGATCTTTTAAAATCT+AGG + chr1_2:24717923-24717942 Msa0063950:intron 20.0%
!! TAAAATTTCAACAATTTGGT+TGG - chr1_2:24718038-24718057 None:intergenic 20.0%
!!! TTGAGACTAAATTAATTTGT+AGG - chr1_2:24718143-24718162 None:intergenic 20.0%
!!! GACAAATGAACTATTTTTAA+GGG - chr1_2:24718325-24718344 None:intergenic 20.0%
!!! AGTCAAATGTTTTTAATGAT+CGG - chr1_2:24718914-24718933 None:intergenic 20.0%
!! ATTGTGAAATTGAATAAGAA+AGG + chr1_2:24719715-24719734 Msa0063950:intron 20.0%
!!! CTCTTGTATTATTATTTTTG+AGG + chr1_2:24720262-24720281 Msa0063950:three_prime_UTR 20.0%
!!! TTGTATCATTCCAATTTTAT+CGG + chr1_2:24720362-24720381 Msa0063950:three_prime_UTR 20.0%
!! ATCCAAAAATGACAATTTAA+CGG - chr1_2:24720421-24720440 None:intergenic 20.0%
! CTAAACTAAACCTTAAATCT+TGG + chr1_2:24717342-24717361 Msa0063950:five_prime_UTR 25.0%
! GTTATAAACAAGAAATGAAG+GGG - chr1_2:24717757-24717776 None:intergenic 25.0%
! CTGTTATAAACAAGAAATGA+AGG - chr1_2:24717759-24717778 None:intergenic 25.0%
!! CTTAGAACACTGAAATATTT+TGG - chr1_2:24717845-24717864 None:intergenic 25.0%
! TCTTGTAAACAATTTCCATA+TGG - chr1_2:24717910-24717929 None:intergenic 25.0%
!!! AAGATCTTTTAAAATCTAGG+AGG + chr1_2:24717926-24717945 Msa0063950:intron 25.0%
!!! AGATCTTTTAAAATCTAGGA+GGG + chr1_2:24717927-24717946 Msa0063950:intron 25.0%
!!! TGTTGAAATTTTAGTTTGAC+AGG + chr1_2:24718045-24718064 Msa0063950:intron 25.0%
! AGATGTTAAATTGACATGTT+TGG + chr1_2:24718068-24718087 Msa0063950:intron 25.0%
!!! TCAGCTAGAATTTAAGTTTT+TGG + chr1_2:24718117-24718136 Msa0063950:intron 25.0%
! TTACGCTTTCATATAAAAGT+AGG - chr1_2:24718182-24718201 None:intergenic 25.0%
!!! AAATGAACTATTTTTAAGGG+AGG - chr1_2:24718322-24718341 None:intergenic 25.0%
!!! CGACAAATGAACTATTTTTA+AGG - chr1_2:24718326-24718345 None:intergenic 25.0%
! ATGAAATTGAAGTCTAAGAA+AGG + chr1_2:24718534-24718553 Msa0063950:CDS 25.0%
!! GATTTTCCTTTTAAATGAAG+AGG - chr1_2:24718576-24718595 None:intergenic 25.0%
! TCATTTAAAAGGAAAATCAC+CGG + chr1_2:24718578-24718597 Msa0063950:CDS 25.0%
! TCATACACATTAAGATAAAC+TGG - chr1_2:24718663-24718682 None:intergenic 25.0%
!!! GTCAAATGTTTTTAATGATC+GGG - chr1_2:24718913-24718932 None:intergenic 25.0%
! CAAATGAAGAAGAAAAACAT+TGG - chr1_2:24719882-24719901 None:intergenic 25.0%
!! CAATGTTTTTCTTCTTCATT+TGG + chr1_2:24719880-24719899 Msa0063950:intron 25.0%
! TTGTAGAAACAATGAAGATT+TGG - chr1_2:24720091-24720110 None:intergenic 25.0%
!!! TTCCGTTAAATTGTCATTTT+TGG + chr1_2:24720416-24720435 Msa0063950:three_prime_UTR 25.0%
!! GTTTGTTATTGCGTAGTTTA+TGG + chr1_2:24717270-24717289 Msa0063950:five_prime_UTR 30.0%
ATGAAACATGCCAAGATTTA+AGG - chr1_2:24717355-24717374 None:intergenic 30.0%
GTCATGAATAGTTTCAAAAG+TGG - chr1_2:24717570-24717589 None:intergenic 30.0%
!!! GATCTTTTAAAATCTAGGAG+GGG + chr1_2:24717928-24717947 Msa0063950:intron 30.0%
GCTTTCATATAAAAGTAGGT+TGG - chr1_2:24718178-24718197 None:intergenic 30.0%
!! TTGTTTAGTATCGACATGTA+AGG + chr1_2:24718287-24718306 Msa0063950:intron 30.0%
TCCTGAATTATCTCATAAGA+AGG + chr1_2:24718373-24718392 Msa0063950:intron 30.0%
TCCTTCTTATGAGATAATTC+AGG - chr1_2:24718377-24718396 None:intergenic 30.0%
TTCAATTTCATCTTTCCCTA+AGG - chr1_2:24718525-24718544 None:intergenic 30.0%
AATTGAAGTCTAAGAAAGGA+TGG + chr1_2:24718538-24718557 Msa0063950:CDS 30.0%
ATTGTGCCTCTTCATTTAAA+AGG + chr1_2:24718567-24718586 Msa0063950:CDS 30.0%
CAATACACATAACCATTCAT+GGG - chr1_2:24718693-24718712 None:intergenic 30.0%
CATGAATGGTTATGTGTATT+GGG + chr1_2:24718692-24718711 Msa0063950:CDS 30.0%
GCATATGACTTAAATTAACC+TGG + chr1_2:24718805-24718824 Msa0063950:intron 30.0%
TGAACTGTAAAATACAGGTA+TGG - chr1_2:24719041-24719060 None:intergenic 30.0%
! TACCTGTATTTTACAGTTCA+TGG + chr1_2:24719041-24719060 Msa0063950:intron 30.0%
AAGAATATCCTAAAGACTAG+AGG - chr1_2:24719409-24719428 None:intergenic 30.0%
!!! GTCTTTAGGATATTCTTTTG+CGG + chr1_2:24719412-24719431 Msa0063950:intron 30.0%
!!! TCTTTAGGATATTCTTTTGC+GGG + chr1_2:24719413-24719432 Msa0063950:intron 30.0%
! GCTTTGAATCAGATATTGAT+TGG + chr1_2:24719514-24719533 Msa0063950:intron 30.0%
CTTATTCAATTTCACAATCC+AGG - chr1_2:24719713-24719732 None:intergenic 30.0%
!!! AATAACTTTTAGCCTTATGC+CGG + chr1_2:24719780-24719799 Msa0063950:intron 30.0%
AGAAGAAAAACATTGGCATA+AGG - chr1_2:24719875-24719894 None:intergenic 30.0%
! CACATGATAGTGAAAAAAGA+AGG + chr1_2:24720010-24720029 Msa0063950:CDS 30.0%
TTTCTACAATCACCTTTAAG+AGG + chr1_2:24720102-24720121 Msa0063950:CDS 30.0%
TGTTTCTTTGTACCTTGAAA+AGG + chr1_2:24720196-24720215 Msa0063950:three_prime_UTR 30.0%
GTTTCTTTGTACCTTGAAAA+GGG + chr1_2:24720197-24720216 Msa0063950:three_prime_UTR 30.0%
! ATTCATTCATACCCTTTTCA+AGG - chr1_2:24720211-24720230 None:intergenic 30.0%
CAATAACAATAGTCCCTTTA+GGG - chr1_2:24720392-24720411 None:intergenic 30.0%
ACAATAACAATAGTCCCTTT+AGG - chr1_2:24720393-24720412 None:intergenic 30.0%
TTATTGAGGTGTAACATTTG+CGG + chr1_2:24720446-24720465 Msa0063950:three_prime_UTR 30.0%
AATTGCAAAATACTCCTCTC+TGG - chr1_2:24717230-24717249 None:intergenic 35.0%
!! AATTTTCCAGATCTAGGACA+TGG - chr1_2:24717657-24717676 None:intergenic 35.0%
! GAGGTTAATTTTCCAGATCT+AGG - chr1_2:24717663-24717682 None:intergenic 35.0%
! CTATTAGGGTTTTCTCCATA+TGG + chr1_2:24717892-24717911 Msa0063950:intron 35.0%
!!! TGAACTATTTTTAAGGGAGG+AGG - chr1_2:24718319-24718338 None:intergenic 35.0%
! CTCAAACATTTGTTGAATGC+AGG + chr1_2:24718482-24718501 Msa0063950:intron 35.0%
AATAACGAAAAACGAGTCAC+CGG - chr1_2:24718600-24718619 None:intergenic 35.0%
AAAGTAAATTCAGCTAGCCA+TGG + chr1_2:24718624-24718643 Msa0063950:CDS 35.0%
! AGATAAACTGGAGTTTTTCC+AGG - chr1_2:24718651-24718670 None:intergenic 35.0%
CCAATACACATAACCATTCA+TGG - chr1_2:24718694-24718713 None:intergenic 35.0%
CCATGAATGGTTATGTGTAT+TGG + chr1_2:24718691-24718710 Msa0063950:CDS 35.0%
ACTACATTTGAGAAAAAGCC+AGG - chr1_2:24718826-24718845 None:intergenic 35.0%
CACCATGAACTGTAAAATAC+AGG - chr1_2:24719046-24719065 None:intergenic 35.0%
CATGGTGTAGAATATGCATT+TGG + chr1_2:24719059-24719078 Msa0063950:CDS 35.0%
TTCAGGAAGTCGATATTCAT+CGG + chr1_2:24719146-24719165 Msa0063950:CDS 35.0%
TACATCTTTGAATCCTGTTC+AGG + chr1_2:24719172-24719191 Msa0063950:CDS 35.0%
! TTTGAATCCTGTTCAGGTTA+GGG + chr1_2:24719178-24719197 Msa0063950:CDS 35.0%
ACATATGCTACAAGCTTACA+GGG + chr1_2:24719282-24719301 Msa0063950:CDS 35.0%
AGGGAAATCAATTCCAACTT+GGG + chr1_2:24719301-24719320 Msa0063950:CDS 35.0%
CAATTCCAACTTGGGTAAAT+CGG + chr1_2:24719309-24719328 Msa0063950:CDS 35.0%
!!! CTTTAGGATATTCTTTTGCG+GGG + chr1_2:24719414-24719433 Msa0063950:intron 35.0%
TCTGATTCAAAGCAATAGCA+AGG - chr1_2:24719507-24719526 None:intergenic 35.0%
ATGGTTAGAGGTTACTGAAT+AGG + chr1_2:24719548-24719567 Msa0063950:intron 35.0%
ATGCATGATCCATATCACAT+GGG - chr1_2:24719653-24719672 None:intergenic 35.0%
GGTCAATTGACTGATTGAAA+TGG + chr1_2:24719736-24719755 Msa0063950:intron 35.0%
CGTTAAGGAGTAGAGTAATT+AGG - chr1_2:24719829-24719848 None:intergenic 35.0%
GAAAAACATTGGCATAAGGT+TGG - chr1_2:24719871-24719890 None:intergenic 35.0%
TATTGACACTTTGTGATGAC+AGG + chr1_2:24719916-24719935 Msa0063950:intron 35.0%
GAAGAAATTCTTAGTGCTTC+TGG - chr1_2:24719964-24719983 None:intergenic 35.0%
AGCACTAAGAATTTCTTCTG+TGG + chr1_2:24719966-24719985 Msa0063950:CDS 35.0%
GCACTAAGAATTTCTTCTGT+GGG + chr1_2:24719967-24719986 Msa0063950:CDS 35.0%
CACTAAGAATTTCTTCTGTG+GGG + chr1_2:24719968-24719987 Msa0063950:CDS 35.0%
GATGATAAACAGCCTCTTAA+AGG - chr1_2:24720117-24720136 None:intergenic 35.0%
ATCTATTAACCGTTCCTTCA+AGG + chr1_2:24720164-24720183 Msa0063950:CDS 35.0%
AAACAGTTACCTTTCCTTGA+AGG - chr1_2:24720181-24720200 None:intergenic 35.0%
ACTTACAAACAGACAAGAGA+TGG - chr1_2:24720295-24720314 None:intergenic 35.0%
TTAGGGACATCCGATAAAAT+TGG - chr1_2:24720375-24720394 None:intergenic 35.0%
! ATTTTATCGGATGTCCCTAA+AGG + chr1_2:24720375-24720394 Msa0063950:three_prime_UTR 35.0%
! TTTTATCGGATGTCCCTAAA+GGG + chr1_2:24720376-24720395 Msa0063950:three_prime_UTR 35.0%
CGTAGTTTATGGTCTATGAC+AGG + chr1_2:24717281-24717300 Msa0063950:five_prime_UTR 40.0%
TATGGTCTATGACAGGACAA+TGG + chr1_2:24717288-24717307 Msa0063950:five_prime_UTR 40.0%
!! AGAGAGAGTGTTGCTCAAAA+CGG - chr1_2:24717390-24717409 None:intergenic 40.0%
CTGTCTTTGTCTCTCTCTAA+TGG + chr1_2:24717408-24717427 Msa0063950:five_prime_UTR 40.0%
TTAATGCCATGTCCTAGATC+TGG + chr1_2:24717648-24717667 Msa0063950:intron 40.0%
AAATCTAGGAGGGGTAGAAT+TGG + chr1_2:24717937-24717956 Msa0063950:intron 40.0%
AAACTTCTGCAAAGCATGGT+TGG - chr1_2:24717986-24718005 None:intergenic 40.0%
AACCATGCTTTGCAGAAGTT+TGG + chr1_2:24717985-24718004 Msa0063950:intron 40.0%
TGACATGTTTGGATGTTCTC+TGG + chr1_2:24718079-24718098 Msa0063950:intron 40.0%
TGGATGTTCTCTGGTAGAAT+TGG + chr1_2:24718088-24718107 Msa0063950:intron 40.0%
ATTATGTGAGTGTTCGCCTT+AGG + chr1_2:24718506-24718525 Msa0063950:five_prime_UTR 40.0%
TTATGTGAGTGTTCGCCTTA+GGG + chr1_2:24718507-24718526 Msa0063950:five_prime_UTR 40.0%
TATGACTTGACACCCATGAA+TGG + chr1_2:24718678-24718697 Msa0063950:CDS 40.0%
AATGGTTATGTGTATTGGGC+CGG + chr1_2:24718696-24718715 Msa0063950:CDS 40.0%
AGAGTGATAGATACCAAGAC+CGG - chr1_2:24718718-24718737 None:intergenic 40.0%
CATGCTCTGTCTCTTAATCT+TGG + chr1_2:24718783-24718802 Msa0063950:intron 40.0%
!!! GTGTTTTTGAGGTTGAACCT+CGG + chr1_2:24719105-24719124 Msa0063950:CDS 40.0%
! CTTTGAATCCTGTTCAGGTT+AGG + chr1_2:24719177-24719196 Msa0063950:CDS 40.0%
TGTGAGTGTGCAAACTACAA+CGG + chr1_2:24719212-24719231 Msa0063950:CDS 40.0%
GACATATGCTACAAGCTTAC+AGG + chr1_2:24719281-24719300 Msa0063950:CDS 40.0%
CAGGGAAATCAATTCCAACT+TGG + chr1_2:24719300-24719319 Msa0063950:CDS 40.0%
! TGTTGCAACCTCTAGTCTTT+AGG + chr1_2:24719398-24719417 Msa0063950:intron 40.0%
! TTTTGCGGGGAAGGAATAAA+AGG + chr1_2:24719427-24719446 Msa0063950:intron 40.0%
! ACATCACACACACACAGTTT+TGG - chr1_2:24719469-24719488 None:intergenic 40.0%
CATGCATGATCCATATCACA+TGG - chr1_2:24719654-24719673 None:intergenic 40.0%
GATCGCATTCTGAGCAAAAT+CGG + chr1_2:24719675-24719694 Msa0063950:intron 40.0%
AATCGGAGTGAAATAGATCC+TGG + chr1_2:24719692-24719711 Msa0063950:intron 40.0%
AAAAAGTTCATCGCCACTAC+CGG - chr1_2:24719802-24719821 None:intergenic 40.0%
! ACATTGGCATAAGGTTGGTT+TGG - chr1_2:24719866-24719885 None:intergenic 40.0%
CTATCATGTGCCTCATGATT+AGG - chr1_2:24720000-24720019 None:intergenic 40.0%
GAGGCTGTTTATCATCAAAG+TGG + chr1_2:24720121-24720140 Msa0063950:CDS 40.0%
TATCATCAAAGTGGCCATCA+TGG + chr1_2:24720130-24720149 Msa0063950:CDS 40.0%
ATCATCAAAGTGGCCATCAT+GGG + chr1_2:24720131-24720150 Msa0063950:CDS 40.0%
! GATTTGCTCAATTCCCATGA+TGG - chr1_2:24720147-24720166 None:intergenic 40.0%
TTAACCGTTCCTTCAAGGAA+AGG + chr1_2:24720169-24720188 Msa0063950:CDS 40.0%
GTTACCTTTCCTTGAAGGAA+CGG - chr1_2:24720176-24720195 None:intergenic 40.0%
TGTTGCTCAAAACGGTGAAG+TGG - chr1_2:24717382-24717401 None:intergenic 45.0%
TCACAGCACAGATACTAGAG+AGG - chr1_2:24717682-24717701 None:intergenic 45.0%
GACCAAACTTCTGCAAAGCA+TGG - chr1_2:24717990-24718009 None:intergenic 45.0%
GGTCTTGGTATCTATCACTC+TGG + chr1_2:24718717-24718736 Msa0063950:CDS 45.0%
! ATCTATCACTCTGGTGTCGA+AGG + chr1_2:24718726-24718745 Msa0063950:CDS 45.0%
CCTCAAAAACACCGCTTGTT+GGG - chr1_2:24719097-24719116 None:intergenic 45.0%
ACCTCAAAAACACCGCTTGT+TGG - chr1_2:24719098-24719117 None:intergenic 45.0%
ACTTCCTGAACTTGAAGCCT+GGG - chr1_2:24719136-24719155 None:intergenic 45.0%
ATGAACTCCCTAACCTGAAC+AGG - chr1_2:24719188-24719207 None:intergenic 45.0%
! TGTTCAGGTTAGGGAGTTCA+TGG + chr1_2:24719187-24719206 Msa0063950:CDS 45.0%
!! GTAAATCGGCTTGCTAAGCT+AGG + chr1_2:24719323-24719342 Msa0063950:CDS 45.0%
! AGGATATTCTTTTGCGGGGA+AGG + chr1_2:24719418-24719437 Msa0063950:intron 45.0%
!!! CACACACACACAGTTTTGGA+TGG - chr1_2:24719465-24719484 None:intergenic 45.0%
!! TTGATTGGCCTGAAGCTAGA+TGG + chr1_2:24719529-24719548 Msa0063950:intron 45.0%
! ACCTCTAACCATCTAGCTTC+AGG - chr1_2:24719540-24719559 None:intergenic 45.0%
CTTACCAGTCCCATGTGATA+TGG + chr1_2:24719641-24719660 Msa0063950:intron 45.0%
TGATCCATATCACATGGGAC+TGG - chr1_2:24719648-24719667 None:intergenic 45.0%
!!! TTTTAGCCTTATGCCGGTAG+TGG + chr1_2:24719786-24719805 Msa0063950:intron 45.0%
!! ATAAGGTTGGTTTGGTGGCT+AGG - chr1_2:24719858-24719877 None:intergenic 45.0%
!! TTGGCATAAGGTTGGTTTGG+TGG - chr1_2:24719863-24719882 None:intergenic 45.0%
AATTCAGCTAGCCATGGACC+TGG + chr1_2:24718630-24718649 Msa0063950:CDS 50.0%
! CTGGAGTTTTTCCAGGTCCA+TGG - chr1_2:24718644-24718663 None:intergenic 50.0%
TATGTGTATTGGGCCGGTCT+TGG + chr1_2:24718702-24718721 Msa0063950:CDS 50.0%
GCTCATGACTACCCAACAAG+CGG + chr1_2:24719083-24719102 Msa0063950:CDS 50.0%
!! CCCAACAAGCGGTGTTTTTG+AGG + chr1_2:24719094-24719113 Msa0063950:CDS 50.0%
! AATGCCCAGGCTTCAAGTTC+AGG + chr1_2:24719129-24719148 Msa0063950:CDS 50.0%
GACTTCCTGAACTTGAAGCC+TGG - chr1_2:24719137-24719156 None:intergenic 50.0%
! GCAAGCCGATTTACCCAAGT+TGG - chr1_2:24719317-24719336 None:intergenic 50.0%
GCCTGAAGCTAGATGGTTAG+AGG + chr1_2:24719536-24719555 Msa0063950:intron 50.0%
GGGGCATGATCCTAATCATG+AGG + chr1_2:24719987-24720006 Msa0063950:CDS 50.0%
TCATCGCCACTACCGGCATA+AGG - chr1_2:24719795-24719814 None:intergenic 55.0%
!! TTGGTTTGGTGGCTAGGTGG+AGG - chr1_2:24719852-24719871 None:intergenic 55.0%
!! AGGTTGGTTTGGTGGCTAGG+TGG - chr1_2:24719855-24719874 None:intergenic 55.0%
GTTGAACCTCGGCAATGCCC+AGG + chr1_2:24719116-24719135 Msa0063950:CDS 60.0%
! TTGAAGCCTGGGCATTGCCG+AGG - chr1_2:24719125-24719144 None:intergenic 60.0%
!! GTGGCTAGGTGGAGGCGTTA+AGG - chr1_2:24719844-24719863 None:intergenic 60.0%
Chromosome Type Strat End Strand Name
chr1_2 gene 24717218 24720480 24717218 ID=Msa0063950;Name=Msa0063950
chr1_2 mRNA 24717218 24720480 24717218 ID=Msa0063950-mRNA-1;Parent=Msa0063950;Name=Msa0063950-mRNA-1;_AED=0.20;_eAED=0.20;_QI=297|1|1|1|0.5|0.4|5|286|252
chr1_2 exon 24717218 24717453 24717218 ID=Msa0063950-mRNA-1:exon:8864;Parent=Msa0063950-mRNA-1
chr1_2 exon 24718392 24718422 24718392 ID=Msa0063950-mRNA-1:exon:8865;Parent=Msa0063950-mRNA-1
chr1_2 exon 24718504 24718747 24718504 ID=Msa0063950-mRNA-1:exon:8866;Parent=Msa0063950-mRNA-1
chr1_2 exon 24719057 24719344 24719057 ID=Msa0063950-mRNA-1:exon:8867;Parent=Msa0063950-mRNA-1
chr1_2 exon 24719938 24720480 24719938 ID=Msa0063950-mRNA-1:exon:8868;Parent=Msa0063950-mRNA-1
chr1_2 five_prime_UTR 24717218 24717453 24717218 ID=Msa0063950-mRNA-1:five_prime_utr;Parent=Msa0063950-mRNA-1
chr1_2 five_prime_UTR 24718392 24718422 24718392 ID=Msa0063950-mRNA-1:five_prime_utr;Parent=Msa0063950-mRNA-1
chr1_2 five_prime_UTR 24718504 24718533 24718504 ID=Msa0063950-mRNA-1:five_prime_utr;Parent=Msa0063950-mRNA-1
chr1_2 CDS 24718534 24718747 24718534 ID=Msa0063950-mRNA-1:cds;Parent=Msa0063950-mRNA-1
chr1_2 CDS 24719057 24719344 24719057 ID=Msa0063950-mRNA-1:cds;Parent=Msa0063950-mRNA-1
chr1_2 CDS 24719938 24720194 24719938 ID=Msa0063950-mRNA-1:cds;Parent=Msa0063950-mRNA-1
chr1_2 three_prime_UTR 24720195 24720480 24720195 ID=Msa0063950-mRNA-1:three_prime_utr;Parent=Msa0063950-mRNA-1
Gene Sequence

>Msa0063950

ATGAAATTGAAGTCTAAGAAAGGATGGAAATCTATTGTGCCTCTTCATTTAAAAGGAAAATCACCGGTGACTCGTTTTTCGTTATTTCGCAAAGTAAATTCAGCTAGCCATGGACCTGGAAAAACTCCAGTTTATCTTAATGTGTATGACTTGACACCCATGAATGGTTATGTGTATTGGGCCGGTCTTGGTATCTATCACTCTGGTGTCGAAGTTCATGGTGTAGAATATGCATTTGGAGCTCATGACTACCCAACAAGCGGTGTTTTTGAGGTTGAACCTCGGCAATGCCCAGGCTTCAAGTTCAGGAAGTCGATATTCATCGGAACTACATCTTTGAATCCTGTTCAGGTTAGGGAGTTCATGGAATGTGAGTGTGCAAACTACAACGGCGACACATATCACTTAATTGCTAAGAACTGCAATCACTTCTGCAAAGACATATGCTACAAGCTTACAGGGAAATCAATTCCAACTTGGGTAAATCGGCTTGCTAAGCTAGGTTCAATTTGCAACTGCATTCTTCCAGAAGCACTAAGAATTTCTTCTGTGGGGCATGATCCTAATCATGAGGCACATGATAGTGAAAAAAGAAGGCTAAGAAGTGCTTTCAATTCATTGTCTTCAATCTCAATGCGTCAAAAGCAGTTATCCAAATCTTCATTGTTTCTACAATCACCTTTAAGAGGCTGTTTATCATCAAAGTGGCCATCATGGGAATTGAGCAAATCTATTAACCGTTCCTTCAAGGAAAGGTAA
Protein sequence

>Msa0063950

MKLKSKKGWKSIVPLHLKGKSPVTRFSLFRKVNSASHGPGKTPVYLNVYDLTPMNGYVYWAGLGIYHSGVEVHGVEYAFGAHDYPTSGVFEVEPRQCPGFKFRKSIFIGTTSLNPVQVREFMECECANYNGDTYHLIAKNCNHFCKDICYKLTGKSIPTWVNRLAKLGSICNCILPEALRISSVGHDPNHEAHDSEKRRLRSAFNSLSSISMRQKQLSKSSLFLQSPLRGCLSSKWPSWELSKSINRSFKER*