AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa0063870

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0063870 MtrunA17_Chr1g0154681 99.603 252 1 0 1 252 1 252 0.0 521
Msa0063870 MtrunA17_Chr1g0162711 70.000 240 67 2 1 240 1 235 9.85e-123 349
Msa0063870 MtrunA17_Chr1g0149791 57.216 194 75 1 39 232 14 199 4.63e-81 242
Msa0063870 MtrunA17_Chr3g0137121 64.024 164 59 0 39 202 14 177 2.89e-80 240
Msa0063870 MtrunA17_Chr4g0055821 64.000 150 54 0 41 190 21 170 7.02e-74 224
Msa0063870 MtrunA17_Chr5g0399541 62.162 148 56 0 41 188 22 169 7.84e-72 219
Msa0063870 MtrunA17_Chr4g0002531 54.595 185 80 2 25 206 10 193 3.18e-69 213
Msa0063870 MtrunA17_Chr4g0063261 58.385 161 66 1 44 203 31 191 8.63e-68 209
Msa0063870 MtrunA17_Chr2g0306871 48.352 182 76 1 49 212 33 214 8.13e-65 201
Msa0063870 MtrunA17_Chr1g0202701 34.965 143 74 5 44 170 4 143 2.46e-18 81.3
Msa0063870 MtrunA17_Chr6g0468771 33.103 145 81 4 42 171 2 145 1.41e-17 79.7
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0063870 AT1G47740.1 71.875 256 63 4 1 252 29 279 7.08e-127 361
Msa0063870 AT1G47740.2 71.875 256 63 4 1 252 29 279 7.08e-127 361
Msa0063870 AT1G47740.3 71.875 256 63 4 1 252 29 279 7.08e-127 361
Msa0063870 AT5G25170.1 63.855 166 58 1 39 202 15 180 5.13e-77 232
Msa0063870 AT1G80690.1 56.044 182 79 1 28 209 2 182 4.97e-74 225
Msa0063870 AT2G25190.1 60.736 163 64 0 40 202 15 177 2.42e-73 224
Msa0063870 AT5G47310.1 64.828 145 51 0 42 186 28 172 9.30e-73 223
Msa0063870 AT1G80690.2 54.839 186 79 2 28 209 2 186 4.81e-72 220
Msa0063870 AT4G17486.1 58.974 156 64 0 33 188 17 172 1.35e-71 219
Msa0063870 AT4G31980.1 59.756 164 58 2 27 187 7 165 2.25e-63 210
Msa0063870 AT4G17486.2 60.145 138 55 0 33 170 17 154 2.17e-62 194
Msa0063870 AT5G25170.2 58.519 135 54 1 70 202 2 136 2.69e-53 170
Msa0063870 AT4G25680.1 32.639 144 87 4 28 169 8 143 4.06e-16 75.9
Msa0063870 AT4G25660.1 32.639 144 87 4 28 169 8 143 9.63e-16 75.1

Find 76 sgRNAs with CRISPR-Local

Find 186 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
ATGAAACATGCCAGGATTTA+AGG 0.146859 1_2:-24626976 None:intergenic
GATGATAAACAGCCTCTTAA+AGG 0.165224 1_2:-24629735 None:intergenic
GAAGAAATTCTTAGTGCTTC+TGG 0.179953 1_2:-24629582 None:intergenic
GTTTGTTATTGCGTAGTTTA+TGG 0.182070 1_2:+24626894 Msa0063870:five_prime_UTR
TTAGGGACATCCGATAAAAT+TGG 0.248199 1_2:-24629992 None:intergenic
ATTGTGCCTCTTCATTTAAA+AGG 0.274662 1_2:+24628194 Msa0063870:CDS
ACAATAACAATAGTCCCTTT+AGG 0.301623 1_2:-24630010 None:intergenic
GACATATGCTACAAGCTTAC+AGG 0.302536 1_2:+24628908 Msa0063870:CDS
CTTTGAATCCTGTTCAGGTT+AGG 0.313137 1_2:+24628804 Msa0063870:CDS
CTGTCTTTGTCTCTCTCTAA+TGG 0.319836 1_2:+24627032 Msa0063870:five_prime_UTR
TACATCTTTGAATCCTGTTC+AGG 0.336718 1_2:+24628799 Msa0063870:CDS
CTATCATGTGCCTCATGATT+AGG 0.345697 1_2:-24629618 None:intergenic
ATCTATTAACCGTTCCTTCA+AGG 0.354021 1_2:+24629785 Msa0063870:CDS
CAATTCCAACTTGGGTAAAT+CGG 0.354741 1_2:+24628936 Msa0063870:CDS
CAATAACAATAGTCCCTTTA+GGG 0.367363 1_2:-24630009 None:intergenic
TCATACACATTAAGATAAAC+TGG 0.373779 1_2:-24628287 None:intergenic
CCAATACACATAACCATTCA+TGG 0.376837 1_2:-24628318 None:intergenic
TGTTCAGGTTAGGGAGTTCA+TGG 0.381216 1_2:+24628814 Msa0063870:CDS
AAACAGTTACCTTTCCTTGA+AGG 0.382205 1_2:-24629799 None:intergenic
TTTGAATCCTGTTCAGGTTA+GGG 0.385241 1_2:+24628805 Msa0063870:CDS
TTTCTACAATCACCTTTAAG+AGG 0.386624 1_2:+24629723 Msa0063870:CDS
TGTTTCTTTGTACCTTGAAA+AGG 0.387538 1_2:+24629817 Msa0063870:three_prime_UTR
ATTATGTGAGTGTTCGCCTT+AGG 0.389160 1_2:+24628133 Msa0063870:five_prime_UTR
TTGTAGAAACAATGAAGATT+TGG 0.406339 1_2:-24629709 None:intergenic
CATGGTGTAGAATATGCATT+TGG 0.412888 1_2:+24628686 Msa0063870:CDS
CAGGGAAATCAATTCCAACT+TGG 0.414012 1_2:+24628927 Msa0063870:CDS
CATGAATGGTTATGTGTATT+GGG 0.415496 1_2:+24628319 Msa0063870:CDS
TATGTGTATTGGGCCGGTCT+TGG 0.423862 1_2:+24628329 Msa0063870:CDS
CCATGAATGGTTATGTGTAT+TGG 0.424620 1_2:+24628318 Msa0063870:CDS
ATGAACTCCCTAACCTGAAC+AGG 0.425276 1_2:-24628812 None:intergenic
GCAAGCCGATTTACCCAAGT+TGG 0.426198 1_2:-24628941 None:intergenic
AATGCCCAGGCTTCAAGTTC+AGG 0.427519 1_2:+24628756 Msa0063870:CDS
GTTTCTTTGTACCTTGAAAA+GGG 0.454647 1_2:+24629818 Msa0063870:three_prime_UTR
TTCAATTTCATCTTTCCCTA+AGG 0.460939 1_2:-24628149 None:intergenic
TTATGTGAGTGTTCGCCTTA+GGG 0.463750 1_2:+24628134 Msa0063870:five_prime_UTR
GACTTCCTGAACTTGAAGCC+TGG 0.467872 1_2:-24628761 None:intergenic
TATCATCAAAGTGGCCATCA+TGG 0.473681 1_2:+24629751 Msa0063870:CDS
GCACTAAGAATTTCTTCTGT+GGG 0.486160 1_2:+24629588 Msa0063870:CDS
TTATTGAGGTGTAACATTTG+CGG 0.489640 1_2:+24630066 Msa0063870:three_prime_UTR
CTAAACTAAACCTTAAATCC+TGG 0.494004 1_2:+24626966 Msa0063870:five_prime_UTR
AATTGCAAAATACTCCTCTC+TGG 0.498588 1_2:-24626851 None:intergenic
ATGAAATTGAAGTCTAAGAA+AGG 0.507796 1_2:+24628161 Msa0063870:CDS
ACTTCCTGAACTTGAAGCCT+GGG 0.521778 1_2:-24628760 None:intergenic
GATTTGCTCAATTCCCATGA+TGG 0.523695 1_2:-24629765 None:intergenic
AATTGAAGTCTAAGAAAGGA+TGG 0.524868 1_2:+24628165 Msa0063870:CDS
GTTGAACCTCGGCAATGCCC+AGG 0.525057 1_2:+24628743 Msa0063870:CDS
ATCTATCACTCTGGTGTCGA+AGG 0.531982 1_2:+24628353 Msa0063870:CDS
CGTAGTTTATGGTCTATGAC+AGG 0.531999 1_2:+24626905 Msa0063870:five_prime_UTR
ATCATCAAAGTGGCCATCAT+GGG 0.535412 1_2:+24629752 Msa0063870:CDS
TTAACCGTTCCTTCAAGGAA+AGG 0.547458 1_2:+24629790 Msa0063870:CDS
AGAGAGAGTGTTGCTCAAAA+CGG 0.548157 1_2:-24627011 None:intergenic
AGGGAAATCAATTCCAACTT+GGG 0.551036 1_2:+24628928 Msa0063870:CDS
GGTCTTGGTATCTATCACTC+TGG 0.557082 1_2:+24628344 Msa0063870:CDS
GTAAATCGGCTTGCTAAGCT+AGG 0.560765 1_2:+24628950 Msa0063870:CDS
TTCAGGAAGTCGATATTCAT+CGG 0.566691 1_2:+24628773 Msa0063870:CDS
CAATACACATAACCATTCAT+GGG 0.567668 1_2:-24628317 None:intergenic
GTTACCTTTCCTTGAAGGAA+CGG 0.569265 1_2:-24629794 None:intergenic
TCATTTAAAAGGAAAATCAC+CGG 0.579187 1_2:+24628205 Msa0063870:CDS
TTGAAGCCTGGGCATTGCCG+AGG 0.584621 1_2:-24628749 None:intergenic
AATTCAGCTAGCCATGGACC+TGG 0.589659 1_2:+24628257 Msa0063870:CDS
AATGGTTATGTGTATTGGGC+CGG 0.593181 1_2:+24628323 Msa0063870:CDS
ACTTACAAACAGACAAGAGA+TGG 0.594126 1_2:-24629913 None:intergenic
TATGACTTGACACCCATGAA+TGG 0.598526 1_2:+24628305 Msa0063870:CDS
ACATATGCTACAAGCTTACA+GGG 0.604015 1_2:+24628909 Msa0063870:CDS
AGCACTAAGAATTTCTTCTG+TGG 0.616876 1_2:+24629587 Msa0063870:CDS
GTGGTGAAATGAAACATGCC+AGG 0.629387 1_2:-24626984 None:intergenic
GAGGCTGTTTATCATCAAAG+TGG 0.631803 1_2:+24629742 Msa0063870:CDS
CACTAAGAATTTCTTCTGTG+GGG 0.635774 1_2:+24629589 Msa0063870:CDS
AGAGTGATAGATACCAAGAC+CGG 0.635910 1_2:-24628342 None:intergenic
AAAGTAAATTCAGCTAGCCA+TGG 0.644369 1_2:+24628251 Msa0063870:CDS
AATAAGGGAGAGAGCCAGAG+AGG 0.645844 1_2:+24626837 None:intergenic
TATGGTCTATGACAGGACAA+TGG 0.650654 1_2:+24626912 Msa0063870:five_prime_UTR
TGTTGCTCAAAACGGTGAAG+TGG 0.655858 1_2:-24627003 None:intergenic
GGGGCATGATCCTAATCATG+AGG 0.671130 1_2:+24629608 Msa0063870:CDS
GCTCATGACTACCCAACAAG+CGG 0.680057 1_2:+24628710 Msa0063870:CDS
TGTGAGTGTGCAAACTACAA+CGG 0.683483 1_2:+24628839 Msa0063870:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!!! ATTTTTATTTAATTATTTAT+TGG + chr1_2:24629956-24629975 Msa0063870:three_prime_UTR 0.0%
!! TTAAAAATAAATAACTTACT+TGG - chr1_2:24628049-24628068 None:intergenic 10.0%
!! ATAATGTTAACATAATAAAT+AGG + chr1_2:24628075-24628094 Msa0063870:intron 10.0%
!!! TTCTACACAATTTTTTTATT+TGG + chr1_2:24627418-24627437 Msa0063870:intron 15.0%
!! AGTATAATAAGAATCTATTA+GGG + chr1_2:24627505-24627524 Msa0063870:intron 15.0%
!! AAACTAAAATTTCAACAATT+TGG - chr1_2:24627669-24627688 None:intergenic 15.0%
!! TTAATTAACAATATTAGCAT+TGG - chr1_2:24629383-24629402 None:intergenic 15.0%
!!! TTTTTGGATATTATTTATTG+AGG + chr1_2:24630052-24630071 Msa0063870:three_prime_UTR 15.0%
!! TGTTATAAACAAGAAATGAA+GGG - chr1_2:24627385-24627404 None:intergenic 20.0%
!! GTCAAAAAAAAAAAGAACAA+TGG - chr1_2:24627450-24627469 None:intergenic 20.0%
!! CAGTATAATAAGAATCTATT+AGG + chr1_2:24627504-24627523 Msa0063870:intron 20.0%
!!! TACAAGATCTTTTAAAATCT+AGG + chr1_2:24627550-24627569 Msa0063870:intron 20.0%
!! TAAAATTTCAACAATTTGGT+TGG - chr1_2:24627665-24627684 None:intergenic 20.0%
!!! TTGAGACTAAATTAATTTGT+AGG - chr1_2:24627770-24627789 None:intergenic 20.0%
!!! GACAAATGAACTATTTTTAA+GGG - chr1_2:24627952-24627971 None:intergenic 20.0%
!!! AGTCAAATGTTTTTAATGAT+CGG - chr1_2:24628541-24628560 None:intergenic 20.0%
!! ATTGTGAAATTGAATAAGAA+AGG + chr1_2:24629336-24629355 Msa0063870:intron 20.0%
!!! CTCTTGTATTATTATTTTTG+AGG + chr1_2:24629883-24629902 Msa0063870:three_prime_UTR 20.0%
!!! TTGTATCATTCCAATTTTAT+CGG + chr1_2:24629982-24630001 Msa0063870:three_prime_UTR 20.0%
!! ATCCAAAAATGACAATTTAA+CGG - chr1_2:24630041-24630060 None:intergenic 20.0%
! GTTATAAACAAGAAATGAAG+GGG - chr1_2:24627384-24627403 None:intergenic 25.0%
! CTGTTATAAACAAGAAATGA+AGG - chr1_2:24627386-24627405 None:intergenic 25.0%
!! CTTAGAACACTGAAATATTT+TGG - chr1_2:24627472-24627491 None:intergenic 25.0%
! TCTTGTAAACAATTTCCATA+TGG - chr1_2:24627537-24627556 None:intergenic 25.0%
!!! AAGATCTTTTAAAATCTAGG+AGG + chr1_2:24627553-24627572 Msa0063870:intron 25.0%
!!! AGATCTTTTAAAATCTAGGA+GGG + chr1_2:24627554-24627573 Msa0063870:intron 25.0%
!!! TGTTGAAATTTTAGTTTGAC+AGG + chr1_2:24627672-24627691 Msa0063870:intron 25.0%
! AGATGTTAAATTGACATGTT+TGG + chr1_2:24627695-24627714 Msa0063870:intron 25.0%
!!! TCAGCTAGAATTTAAGTTTT+TGG + chr1_2:24627744-24627763 Msa0063870:intron 25.0%
! TTACGCTTTCATATAAAAGT+AGG - chr1_2:24627809-24627828 None:intergenic 25.0%
!!! AAATGAACTATTTTTAAGGG+AGG - chr1_2:24627949-24627968 None:intergenic 25.0%
!!! CGACAAATGAACTATTTTTA+AGG - chr1_2:24627953-24627972 None:intergenic 25.0%
! ATGAAATTGAAGTCTAAGAA+AGG + chr1_2:24628161-24628180 Msa0063870:CDS 25.0%
!! GATTTTCCTTTTAAATGAAG+AGG - chr1_2:24628203-24628222 None:intergenic 25.0%
! TCATTTAAAAGGAAAATCAC+CGG + chr1_2:24628205-24628224 Msa0063870:CDS 25.0%
! TCATACACATTAAGATAAAC+TGG - chr1_2:24628290-24628309 None:intergenic 25.0%
!!! GTCAAATGTTTTTAATGATC+GGG - chr1_2:24628540-24628559 None:intergenic 25.0%
! CAAATGAAGAAGAAAAACAT+TGG - chr1_2:24629503-24629522 None:intergenic 25.0%
!! CAATGTTTTTCTTCTTCATT+TGG + chr1_2:24629501-24629520 Msa0063870:intron 25.0%
! TTGTAGAAACAATGAAGATT+TGG - chr1_2:24629712-24629731 None:intergenic 25.0%
!!! TTCCGTTAAATTGTCATTTT+TGG + chr1_2:24630036-24630055 Msa0063870:three_prime_UTR 25.0%
!! GTTTGTTATTGCGTAGTTTA+TGG + chr1_2:24626894-24626913 Msa0063870:five_prime_UTR 30.0%
CTAAACTAAACCTTAAATCC+TGG + chr1_2:24626966-24626985 Msa0063870:five_prime_UTR 30.0%
GTCATGAATAGTTTCAAAAG+TGG - chr1_2:24627194-24627213 None:intergenic 30.0%
!!! GATCTTTTAAAATCTAGGAG+GGG + chr1_2:24627555-24627574 Msa0063870:intron 30.0%
GCTTTCATATAAAAGTAGGT+TGG - chr1_2:24627805-24627824 None:intergenic 30.0%
!! TTGTTTAGTATCGACATGTA+AGG + chr1_2:24627914-24627933 Msa0063870:intron 30.0%
TCCTGAATTATCTCATAAGA+AGG + chr1_2:24628000-24628019 Msa0063870:intron 30.0%
TCCTTCTTATGAGATAATTC+AGG - chr1_2:24628004-24628023 None:intergenic 30.0%
TTCAATTTCATCTTTCCCTA+AGG - chr1_2:24628152-24628171 None:intergenic 30.0%
AATTGAAGTCTAAGAAAGGA+TGG + chr1_2:24628165-24628184 Msa0063870:CDS 30.0%
ATTGTGCCTCTTCATTTAAA+AGG + chr1_2:24628194-24628213 Msa0063870:CDS 30.0%
CAATACACATAACCATTCAT+GGG - chr1_2:24628320-24628339 None:intergenic 30.0%
CATGAATGGTTATGTGTATT+GGG + chr1_2:24628319-24628338 Msa0063870:CDS 30.0%
GCATATGACTTAAATTAACC+TGG + chr1_2:24628432-24628451 Msa0063870:intron 30.0%
TGAACTGTAAAATACAGGTA+TGG - chr1_2:24628668-24628687 None:intergenic 30.0%
! TACCTGTATTTTACAGTTCA+TGG + chr1_2:24628668-24628687 Msa0063870:intron 30.0%
AAGAATATCCTAAAGACTAG+AGG - chr1_2:24629030-24629049 None:intergenic 30.0%
!!! GTCTTTAGGATATTCTTTTG+CGG + chr1_2:24629033-24629052 Msa0063870:intron 30.0%
!!! TCTTTAGGATATTCTTTTGC+GGG + chr1_2:24629034-24629053 Msa0063870:intron 30.0%
! GCTTTGAATCAGATATTGAT+TGG + chr1_2:24629135-24629154 Msa0063870:intron 30.0%
CTTATTCAATTTCACAATCC+AGG - chr1_2:24629334-24629353 None:intergenic 30.0%
!!! AATAACTTTTAGCCTTATGC+CGG + chr1_2:24629401-24629420 Msa0063870:intron 30.0%
AGAAGAAAAACATTGGCATA+AGG - chr1_2:24629496-24629515 None:intergenic 30.0%
! CACATGATAGTGAAAAAAGA+AGG + chr1_2:24629631-24629650 Msa0063870:CDS 30.0%
TTTCTACAATCACCTTTAAG+AGG + chr1_2:24629723-24629742 Msa0063870:CDS 30.0%
TGTTTCTTTGTACCTTGAAA+AGG + chr1_2:24629817-24629836 Msa0063870:three_prime_UTR 30.0%
GTTTCTTTGTACCTTGAAAA+GGG + chr1_2:24629818-24629837 Msa0063870:three_prime_UTR 30.0%
! ATTCATTCATACCCTTTTCA+AGG - chr1_2:24629832-24629851 None:intergenic 30.0%
CAATAACAATAGTCCCTTTA+GGG - chr1_2:24630012-24630031 None:intergenic 30.0%
ACAATAACAATAGTCCCTTT+AGG - chr1_2:24630013-24630032 None:intergenic 30.0%
TTATTGAGGTGTAACATTTG+CGG + chr1_2:24630066-24630085 Msa0063870:three_prime_UTR 30.0%
AATTGCAAAATACTCCTCTC+TGG - chr1_2:24626854-24626873 None:intergenic 35.0%
ATGAAACATGCCAGGATTTA+AGG - chr1_2:24626979-24626998 None:intergenic 35.0%
!! AATTTTCCAGATCTAGGACA+CGG - chr1_2:24627281-24627300 None:intergenic 35.0%
! GAGGTTAATTTTCCAGATCT+AGG - chr1_2:24627287-24627306 None:intergenic 35.0%
! CTATTAGGGTTTTCTCCATA+TGG + chr1_2:24627519-24627538 Msa0063870:intron 35.0%
!!! TGAACTATTTTTAAGGGAGG+AGG - chr1_2:24627946-24627965 None:intergenic 35.0%
! CTCAAACATTTGTTGAATGC+AGG + chr1_2:24628109-24628128 Msa0063870:intron 35.0%
AATAACGAAAAACGAGTCAC+CGG - chr1_2:24628227-24628246 None:intergenic 35.0%
AAAGTAAATTCAGCTAGCCA+TGG + chr1_2:24628251-24628270 Msa0063870:CDS 35.0%
! AGATAAACTGGAGTTTTTCC+AGG - chr1_2:24628278-24628297 None:intergenic 35.0%
CCAATACACATAACCATTCA+TGG - chr1_2:24628321-24628340 None:intergenic 35.0%
CCATGAATGGTTATGTGTAT+TGG + chr1_2:24628318-24628337 Msa0063870:CDS 35.0%
ACTACATTTGAGAAAAAGCC+AGG - chr1_2:24628453-24628472 None:intergenic 35.0%
CACCATGAACTGTAAAATAC+AGG - chr1_2:24628673-24628692 None:intergenic 35.0%
CATGGTGTAGAATATGCATT+TGG + chr1_2:24628686-24628705 Msa0063870:CDS 35.0%
TTCAGGAAGTCGATATTCAT+CGG + chr1_2:24628773-24628792 Msa0063870:CDS 35.0%
TACATCTTTGAATCCTGTTC+AGG + chr1_2:24628799-24628818 Msa0063870:CDS 35.0%
! TTTGAATCCTGTTCAGGTTA+GGG + chr1_2:24628805-24628824 Msa0063870:CDS 35.0%
ACATATGCTACAAGCTTACA+GGG + chr1_2:24628909-24628928 Msa0063870:CDS 35.0%
AGGGAAATCAATTCCAACTT+GGG + chr1_2:24628928-24628947 Msa0063870:CDS 35.0%
CAATTCCAACTTGGGTAAAT+CGG + chr1_2:24628936-24628955 Msa0063870:CDS 35.0%
!!! CTTTAGGATATTCTTTTGCG+GGG + chr1_2:24629035-24629054 Msa0063870:intron 35.0%
TCTGATTCAAAGCAATAGCA+AGG - chr1_2:24629128-24629147 None:intergenic 35.0%
ATGGTTAGAGGTTACTGAAT+AGG + chr1_2:24629169-24629188 Msa0063870:intron 35.0%
ATGCATGATCCATATCACAT+GGG - chr1_2:24629274-24629293 None:intergenic 35.0%
GGTCAATTGACTGATTGAAA+TGG + chr1_2:24629357-24629376 Msa0063870:intron 35.0%
CGTTAAGGAGTAGAGTAATT+AGG - chr1_2:24629450-24629469 None:intergenic 35.0%
GAAAAACATTGGCATAAGGT+TGG - chr1_2:24629492-24629511 None:intergenic 35.0%
TATTGACACTTTGTGATGAC+AGG + chr1_2:24629537-24629556 Msa0063870:intron 35.0%
GAAGAAATTCTTAGTGCTTC+TGG - chr1_2:24629585-24629604 None:intergenic 35.0%
AGCACTAAGAATTTCTTCTG+TGG + chr1_2:24629587-24629606 Msa0063870:CDS 35.0%
GCACTAAGAATTTCTTCTGT+GGG + chr1_2:24629588-24629607 Msa0063870:CDS 35.0%
CACTAAGAATTTCTTCTGTG+GGG + chr1_2:24629589-24629608 Msa0063870:CDS 35.0%
GATGATAAACAGCCTCTTAA+AGG - chr1_2:24629738-24629757 None:intergenic 35.0%
ATCTATTAACCGTTCCTTCA+AGG + chr1_2:24629785-24629804 Msa0063870:CDS 35.0%
AAACAGTTACCTTTCCTTGA+AGG - chr1_2:24629802-24629821 None:intergenic 35.0%
ACTTACAAACAGACAAGAGA+TGG - chr1_2:24629916-24629935 None:intergenic 35.0%
TTAGGGACATCCGATAAAAT+TGG - chr1_2:24629995-24630014 None:intergenic 35.0%
! ATTTTATCGGATGTCCCTAA+AGG + chr1_2:24629995-24630014 Msa0063870:three_prime_UTR 35.0%
! TTTTATCGGATGTCCCTAAA+GGG + chr1_2:24629996-24630015 Msa0063870:three_prime_UTR 35.0%
CGTAGTTTATGGTCTATGAC+AGG + chr1_2:24626905-24626924 Msa0063870:five_prime_UTR 40.0%
TATGGTCTATGACAGGACAA+TGG + chr1_2:24626912-24626931 Msa0063870:five_prime_UTR 40.0%
!! AGAGAGAGTGTTGCTCAAAA+CGG - chr1_2:24627014-24627033 None:intergenic 40.0%
CTGTCTTTGTCTCTCTCTAA+TGG + chr1_2:24627032-24627051 Msa0063870:five_prime_UTR 40.0%
AAATCTAGGAGGGGTAGAAT+TGG + chr1_2:24627564-24627583 Msa0063870:intron 40.0%
AAACTTCTGCAAAGCATGGT+TGG - chr1_2:24627613-24627632 None:intergenic 40.0%
AACCATGCTTTGCAGAAGTT+TGG + chr1_2:24627612-24627631 Msa0063870:intron 40.0%
TGACATGTTTGGATGTTCTC+TGG + chr1_2:24627706-24627725 Msa0063870:intron 40.0%
TGGATGTTCTCTGGTAGAAT+TGG + chr1_2:24627715-24627734 Msa0063870:intron 40.0%
ATTATGTGAGTGTTCGCCTT+AGG + chr1_2:24628133-24628152 Msa0063870:five_prime_UTR 40.0%
TTATGTGAGTGTTCGCCTTA+GGG + chr1_2:24628134-24628153 Msa0063870:five_prime_UTR 40.0%
TATGACTTGACACCCATGAA+TGG + chr1_2:24628305-24628324 Msa0063870:CDS 40.0%
AATGGTTATGTGTATTGGGC+CGG + chr1_2:24628323-24628342 Msa0063870:CDS 40.0%
AGAGTGATAGATACCAAGAC+CGG - chr1_2:24628345-24628364 None:intergenic 40.0%
!! GGTGTCGAAGGTATGATTTT+AGG + chr1_2:24628365-24628384 Msa0063870:intron 40.0%
CATGCTCTGTCTCTTAATCT+TGG + chr1_2:24628410-24628429 Msa0063870:intron 40.0%
!!! GTGTTTTTGAGGTTGAACCT+CGG + chr1_2:24628732-24628751 Msa0063870:CDS 40.0%
! CTTTGAATCCTGTTCAGGTT+AGG + chr1_2:24628804-24628823 Msa0063870:CDS 40.0%
TGTGAGTGTGCAAACTACAA+CGG + chr1_2:24628839-24628858 Msa0063870:CDS 40.0%
GACATATGCTACAAGCTTAC+AGG + chr1_2:24628908-24628927 Msa0063870:CDS 40.0%
CAGGGAAATCAATTCCAACT+TGG + chr1_2:24628927-24628946 Msa0063870:CDS 40.0%
! TGTTGCAACCTCTAGTCTTT+AGG + chr1_2:24629019-24629038 Msa0063870:intron 40.0%
! TTTTGCGGGGAAGGAATAAA+AGG + chr1_2:24629048-24629067 Msa0063870:intron 40.0%
! ACATCACACACACACAGTTT+TGG - chr1_2:24629090-24629109 None:intergenic 40.0%
CATGCATGATCCATATCACA+TGG - chr1_2:24629275-24629294 None:intergenic 40.0%
GATCGCATTCTGAGCAAAAT+CGG + chr1_2:24629296-24629315 Msa0063870:intron 40.0%
AATCGGAGTGAAATAGATCC+TGG + chr1_2:24629313-24629332 Msa0063870:intron 40.0%
AAAAAGTTCATCGCCACTAC+CGG - chr1_2:24629423-24629442 None:intergenic 40.0%
! ACATTGGCATAAGGTTGGTT+TGG - chr1_2:24629487-24629506 None:intergenic 40.0%
CTATCATGTGCCTCATGATT+AGG - chr1_2:24629621-24629640 None:intergenic 40.0%
GAGGCTGTTTATCATCAAAG+TGG + chr1_2:24629742-24629761 Msa0063870:CDS 40.0%
TATCATCAAAGTGGCCATCA+TGG + chr1_2:24629751-24629770 Msa0063870:CDS 40.0%
ATCATCAAAGTGGCCATCAT+GGG + chr1_2:24629752-24629771 Msa0063870:CDS 40.0%
! GATTTGCTCAATTCCCATGA+TGG - chr1_2:24629768-24629787 None:intergenic 40.0%
TTAACCGTTCCTTCAAGGAA+AGG + chr1_2:24629790-24629809 Msa0063870:CDS 40.0%
GTTACCTTTCCTTGAAGGAA+CGG - chr1_2:24629797-24629816 None:intergenic 40.0%
GTGGTGAAATGAAACATGCC+AGG - chr1_2:24626987-24627006 None:intergenic 45.0%
TGTTGCTCAAAACGGTGAAG+TGG - chr1_2:24627006-24627025 None:intergenic 45.0%
TTAATGCCGTGTCCTAGATC+TGG + chr1_2:24627272-24627291 Msa0063870:intron 45.0%
TCACAGCACAGATACTAGAG+AGG - chr1_2:24627306-24627325 None:intergenic 45.0%
GACCAAACTTCTGCAAAGCA+TGG - chr1_2:24627617-24627636 None:intergenic 45.0%
GGTCTTGGTATCTATCACTC+TGG + chr1_2:24628344-24628363 Msa0063870:CDS 45.0%
! ATCTATCACTCTGGTGTCGA+AGG + chr1_2:24628353-24628372 Msa0063870:CDS 45.0%
CCTCAAAAACACCGCTTGTT+GGG - chr1_2:24628724-24628743 None:intergenic 45.0%
ACCTCAAAAACACCGCTTGT+TGG - chr1_2:24628725-24628744 None:intergenic 45.0%
ACTTCCTGAACTTGAAGCCT+GGG - chr1_2:24628763-24628782 None:intergenic 45.0%
ATGAACTCCCTAACCTGAAC+AGG - chr1_2:24628815-24628834 None:intergenic 45.0%
! TGTTCAGGTTAGGGAGTTCA+TGG + chr1_2:24628814-24628833 Msa0063870:CDS 45.0%
!! GTAAATCGGCTTGCTAAGCT+AGG + chr1_2:24628950-24628969 Msa0063870:CDS 45.0%
! AGGATATTCTTTTGCGGGGA+AGG + chr1_2:24629039-24629058 Msa0063870:intron 45.0%
!!! CACACACACACAGTTTTGGA+TGG - chr1_2:24629086-24629105 None:intergenic 45.0%
!! TTGATTGGCCTGAAGCTAGA+TGG + chr1_2:24629150-24629169 Msa0063870:intron 45.0%
! ACCTCTAACCATCTAGCTTC+AGG - chr1_2:24629161-24629180 None:intergenic 45.0%
CTTACCAGTCCCATGTGATA+TGG + chr1_2:24629262-24629281 Msa0063870:intron 45.0%
TGATCCATATCACATGGGAC+TGG - chr1_2:24629269-24629288 None:intergenic 45.0%
!!! TTTTAGCCTTATGCCGGTAG+TGG + chr1_2:24629407-24629426 Msa0063870:intron 45.0%
!! ATAAGGTTGGTTTGGTGGCT+AGG - chr1_2:24629479-24629498 None:intergenic 45.0%
!! TTGGCATAAGGTTGGTTTGG+TGG - chr1_2:24629484-24629503 None:intergenic 45.0%
AATTCAGCTAGCCATGGACC+TGG + chr1_2:24628257-24628276 Msa0063870:CDS 50.0%
! CTGGAGTTTTTCCAGGTCCA+TGG - chr1_2:24628271-24628290 None:intergenic 50.0%
TATGTGTATTGGGCCGGTCT+TGG + chr1_2:24628329-24628348 Msa0063870:CDS 50.0%
GCTCATGACTACCCAACAAG+CGG + chr1_2:24628710-24628729 Msa0063870:CDS 50.0%
!! CCCAACAAGCGGTGTTTTTG+AGG + chr1_2:24628721-24628740 Msa0063870:CDS 50.0%
! AATGCCCAGGCTTCAAGTTC+AGG + chr1_2:24628756-24628775 Msa0063870:CDS 50.0%
GACTTCCTGAACTTGAAGCC+TGG - chr1_2:24628764-24628783 None:intergenic 50.0%
! GCAAGCCGATTTACCCAAGT+TGG - chr1_2:24628944-24628963 None:intergenic 50.0%
GCCTGAAGCTAGATGGTTAG+AGG + chr1_2:24629157-24629176 Msa0063870:intron 50.0%
GGGGCATGATCCTAATCATG+AGG + chr1_2:24629608-24629627 Msa0063870:CDS 50.0%
TCATCGCCACTACCGGCATA+AGG - chr1_2:24629416-24629435 None:intergenic 55.0%
!! TTGGTTTGGTGGCTAGGTGG+AGG - chr1_2:24629473-24629492 None:intergenic 55.0%
!! AGGTTGGTTTGGTGGCTAGG+TGG - chr1_2:24629476-24629495 None:intergenic 55.0%
GTTGAACCTCGGCAATGCCC+AGG + chr1_2:24628743-24628762 Msa0063870:CDS 60.0%
! TTGAAGCCTGGGCATTGCCG+AGG - chr1_2:24628752-24628771 None:intergenic 60.0%
!! GTGGCTAGGTGGAGGCGTTA+AGG - chr1_2:24629465-24629484 None:intergenic 60.0%
Chromosome Type Strat End Strand Name
chr1_2 gene 24626842 24630100 24626842 ID=Msa0063870;Name=Msa0063870
chr1_2 mRNA 24626842 24630100 24626842 ID=Msa0063870-mRNA-1;Parent=Msa0063870;Name=Msa0063870-mRNA-1;_AED=0.20;_eAED=0.20;_QI=297|1|1|1|0.5|0.4|5|285|252
chr1_2 exon 24626842 24627077 24626842 ID=Msa0063870-mRNA-1:exon:8820;Parent=Msa0063870-mRNA-1
chr1_2 exon 24628019 24628049 24628019 ID=Msa0063870-mRNA-1:exon:8821;Parent=Msa0063870-mRNA-1
chr1_2 exon 24628131 24628374 24628131 ID=Msa0063870-mRNA-1:exon:8822;Parent=Msa0063870-mRNA-1
chr1_2 exon 24628684 24628971 24628684 ID=Msa0063870-mRNA-1:exon:8823;Parent=Msa0063870-mRNA-1
chr1_2 exon 24629559 24630100 24629559 ID=Msa0063870-mRNA-1:exon:8824;Parent=Msa0063870-mRNA-1
chr1_2 five_prime_UTR 24626842 24627077 24626842 ID=Msa0063870-mRNA-1:five_prime_utr;Parent=Msa0063870-mRNA-1
chr1_2 five_prime_UTR 24628019 24628049 24628019 ID=Msa0063870-mRNA-1:five_prime_utr;Parent=Msa0063870-mRNA-1
chr1_2 five_prime_UTR 24628131 24628160 24628131 ID=Msa0063870-mRNA-1:five_prime_utr;Parent=Msa0063870-mRNA-1
chr1_2 CDS 24628161 24628374 24628161 ID=Msa0063870-mRNA-1:cds;Parent=Msa0063870-mRNA-1
chr1_2 CDS 24628684 24628971 24628684 ID=Msa0063870-mRNA-1:cds;Parent=Msa0063870-mRNA-1
chr1_2 CDS 24629559 24629815 24629559 ID=Msa0063870-mRNA-1:cds;Parent=Msa0063870-mRNA-1
chr1_2 three_prime_UTR 24629816 24630100 24629816 ID=Msa0063870-mRNA-1:three_prime_utr;Parent=Msa0063870-mRNA-1
Gene Sequence

>Msa0063870

ATGAAATTGAAGTCTAAGAAAGGATGGAAATCTATTGTGCCTCTTCATTTAAAAGGAAAATCACCGGTGACTCGTTTTTCGTTATTTCGCAAAGTAAATTCAGCTAGCCATGGACCTGGAAAAACTCCAGTTTATCTTAATGTGTATGACTTGACACCCATGAATGGTTATGTGTATTGGGCCGGTCTTGGTATCTATCACTCTGGTGTCGAAGTTCATGGTGTAGAATATGCATTTGGAGCTCATGACTACCCAACAAGCGGTGTTTTTGAGGTTGAACCTCGGCAATGCCCAGGCTTCAAGTTCAGGAAGTCGATATTCATCGGAACTACATCTTTGAATCCTGTTCAGGTTAGGGAGTTCATGGAATGTGAGTGTGCAAACTACAACGGCGACACATATCACTTAATTGCTAAGAACTGCAATCACTTCTGCAAAGACATATGCTACAAGCTTACAGGGAAATCAATTCCAACTTGGGTAAATCGGCTTGCTAAGCTAGGTTCAATTTGCAACTGCATTCTTCCAGAAGCACTAAGAATTTCTTCTGTGGGGCATGATCCTAATCATGAGGCACATGATAGTGAAAAAAGAAGGCTAAGAAGTGCTTTCAATTCATTGTCTTCAATCTCAATGCGTCAAAAGCAGTTATCCAAATCTTCATTGTTTCTACAATCACCTTTAAGAGGCTGTTTATCATCAAAGTGGCCATCATGGGAATTGAGCAAATCTATTAACCGTTCCTTCAAGGAAAGGTAA
Protein sequence

>Msa0063870

MKLKSKKGWKSIVPLHLKGKSPVTRFSLFRKVNSASHGPGKTPVYLNVYDLTPMNGYVYWAGLGIYHSGVEVHGVEYAFGAHDYPTSGVFEVEPRQCPGFKFRKSIFIGTTSLNPVQVREFMECECANYNGDTYHLIAKNCNHFCKDICYKLTGKSIPTWVNRLAKLGSICNCILPEALRISSVGHDPNHEAHDSEKRRLRSAFNSLSSISMRQKQLSKSSLFLQSPLRGCLSSKWPSWELSKSINRSFKER*