AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa0076630

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0076630 MtrunA17_Chr1g0185641 100.000 114 0 0 1 114 1 114 2.50e-77 223
Msa0076630 MtrunA17_Chr7g0259691 99.130 115 0 1 1 114 1 115 1.95e-75 218
Msa0076630 MtrunA17_Chr8g0383391 28.319 113 76 2 4 113 9 119 4.93e-12 58.5
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0076630 AT4G02840.1 94.792 96 5 0 1 96 1 96 1.37e-61 184
Msa0076630 AT3G07590.2 89.796 98 10 0 1 98 1 98 1.77e-60 181
Msa0076630 AT3G07590.1 89.796 98 10 0 1 98 1 98 1.77e-60 181
Msa0076630 AT4G02840.2 85.849 106 5 1 1 96 1 106 2.83e-58 176
Msa0076630 AT3G07590.3 88.060 67 8 0 30 96 4 70 9.80e-39 125
Msa0076630 AT1G20580.1 29.524 105 72 1 4 108 9 111 2.10e-12 59.7

Find 67 sgRNAs with CRISPR-Local

Find 291 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
ACTACTTTGTTGACTTATAA+AGG 0.256946 1_2:-52810578 Msa0076630:three_prime_UTR
GTACCAGCAGTTGGCTTCTT+AGG 0.288686 1_2:+52810930 None:intergenic
AAGCACTTGTTCAAATATAA+TGG 0.302741 1_2:+52810644 None:intergenic
ATTGTTCATGGCACCATTAC+AGG 0.313852 1_2:-52814157 Msa0076630:intron
AATGGATCTTATAATCAATT+AGG 0.323511 1_2:-52810489 Msa0076630:three_prime_UTR
TTGGGGCGTGGGCGCGGTCG+AGG 0.328304 1_2:-52810814 Msa0076630:CDS
GGCGGCGATGGGGCGGAAGA+TGG 0.332220 1_2:+52814349 None:intergenic
ATCATCTTAACTAAGGGAAA+TGG 0.333754 1_2:-52810507 Msa0076630:three_prime_UTR
CGTGGGCGCGGTCGAGGGCG+TGG 0.335495 1_2:-52810808 Msa0076630:CDS
CTTGAATCTTGAGACTTTGC+TGG 0.344909 1_2:-52810976 Msa0076630:CDS
TCAGGGAAGCCTTTGGGGCG+TGG 0.359383 1_2:-52810826 Msa0076630:intron
CAGGGAAGCCTTTGGGGCGT+GGG 0.360605 1_2:-52810825 Msa0076630:CDS
GTTGAGACTTGAGTGGTGTT+AGG 0.377675 1_2:+52810552 None:intergenic
AAGCCTTTGGGGCGTGGGCG+CGG 0.383124 1_2:-52810820 Msa0076630:CDS
CGCGGTCGAGGGCGTGGACG+TGG 0.416042 1_2:-52810802 Msa0076630:CDS
ACGAGCTTCATGGCGGCGAT+GGG 0.419657 1_2:+52814338 None:intergenic
GACGAGCTTCATGGCGGCGA+TGG 0.420396 1_2:+52814337 None:intergenic
GCGGCGATGGGGCGGAAGAT+GGG 0.427590 1_2:+52814350 None:intergenic
GTCTCAAGATTCAAGCTGTC+AGG 0.435893 1_2:+52810984 None:intergenic
TTGTGCCATATGGTTGTGTT+GGG 0.447153 1_2:-52810742 Msa0076630:three_prime_UTR
CTGAGATGATCCAGAGTCAC+TGG 0.455517 1_2:+52811041 None:intergenic
TACTAAAACGGTGTCGTACT+CGG 0.455730 1_2:-52814461 Msa0076630:five_prime_UTR
TCGCCGCCATGAAGCTCGTC+AGG 0.455923 1_2:-52814334 Msa0076630:intron
CTGACCCGCTCCTCCCTTCG+TGG 0.458741 1_2:-52814395 Msa0076630:five_prime_UTR
GAGAGACCTGACGAGCTTCA+TGG 0.459293 1_2:+52814328 None:intergenic
CGTGGACGTGGTCGTGGCCG+CGG 0.466756 1_2:-52810790 Msa0076630:CDS
TGGGGCGTGGGCGCGGTCGA+GGG 0.476857 1_2:-52810813 Msa0076630:CDS
CGAGGGCGTGGACGTGGTCG+TGG 0.479147 1_2:-52810796 Msa0076630:CDS
TGGTTGTTGAAGTCGAAAAC+CGG 0.484878 1_2:-52814375 Msa0076630:five_prime_UTR
AAGAACGGTACAATTGTTCA+TGG 0.494710 1_2:-52814169 Msa0076630:CDS
TTTGTGCCATATGGTTGTGT+TGG 0.496936 1_2:-52810743 Msa0076630:three_prime_UTR
TTCTTGCTGAATAGTTATGT+TGG 0.502183 1_2:-52810681 Msa0076630:three_prime_UTR
GGAATTTATGAGTGGTCTTG+TGG 0.505964 1_2:-52810718 Msa0076630:three_prime_UTR
CTCTGGATCATCTCAGTGTG+AGG 0.510476 1_2:-52811034 Msa0076630:CDS
CGACCGCGCCCACGCCCCAA+AGG 0.511469 1_2:+52810817 None:intergenic
GTTCTTGAGCTCGATCGATA+CGG 0.513270 1_2:+52814186 None:intergenic
GGGTTAGTACGAACCTGTAA+TGG 0.514920 1_2:+52814144 None:intergenic
TGAGACTTTGCTGGTTGAAG+AGG 0.514939 1_2:-52810967 Msa0076630:CDS
GGCTTCTTAGGCTTGACCCT+AGG 0.515024 1_2:+52810942 None:intergenic
GCTGGTTGAAGAGGCACCTA+GGG 0.521169 1_2:-52810958 Msa0076630:CDS
TGCCATATGGTTGTGTTGGG+AGG 0.521995 1_2:-52810739 Msa0076630:three_prime_UTR
TTCCTCCCAACACAACCATA+TGG 0.528839 1_2:+52810737 None:intergenic
ACATAAGGGCAATACTAAAA+CGG 0.529212 1_2:-52814473 None:intergenic
AACAGTTAAACTAACACTGA+AGG 0.539350 1_2:-52811072 Msa0076630:CDS
AACAACCACGAAGGGAGGAG+CGG 0.542438 1_2:+52814390 None:intergenic
ACAACCACGAAGGGAGGAGC+GGG 0.547610 1_2:+52814391 None:intergenic
ATGGGGCGGAAGATGGGCGC+CGG 0.548004 1_2:+52814356 None:intergenic
AAGCCTAAGAAGCCAACTGC+TGG 0.548844 1_2:-52810933 Msa0076630:intron
CTCAGTTAAAGAACTCAGAA+AGG 0.555067 1_2:+52810602 None:intergenic
CGAGCTTCATGGCGGCGATG+GGG 0.570180 1_2:+52814339 None:intergenic
GCTTCATGGCGGCGATGGGG+CGG 0.570668 1_2:+52814342 None:intergenic
ACAGTTAAACTAACACTGAA+GGG 0.577307 1_2:-52811071 Msa0076630:CDS
TCGACTTCAACAACCACGAA+GGG 0.578273 1_2:+52814382 None:intergenic
TCTGGATCATCTCAGTGTGA+GGG 0.591054 1_2:-52811033 Msa0076630:CDS
TGCTGGTTGAAGAGGCACCT+AGG 0.593545 1_2:-52810959 Msa0076630:CDS
TAATGTAAAGTACCAGCAGT+TGG 0.601300 1_2:+52810921 None:intergenic
TGTTGGGAGGAATTTATGAG+TGG 0.610163 1_2:-52810726 Msa0076630:three_prime_UTR
GTATCGATCGAGCTCAAGAA+CGG 0.620082 1_2:-52814184 Msa0076630:CDS
CAAGATTCAAGCTGTCAGGA+AGG 0.623754 1_2:+52810988 None:intergenic
CTGGATCATCTCAGTGTGAG+GGG 0.636050 1_2:-52811032 Msa0076630:CDS
CAGTTAAACTAACACTGAAG+GGG 0.636399 1_2:-52811070 Msa0076630:CDS
ACTTCAACAACCACGAAGGG+AGG 0.645006 1_2:+52814385 None:intergenic
TTCGACTTCAACAACCACGA+AGG 0.651469 1_2:+52814381 None:intergenic
TGAGATGATCCAGAGTCACT+GGG 0.651786 1_2:+52811042 None:intergenic
GACTTTAGTTGAGACTTGAG+TGG 0.672289 1_2:+52810545 None:intergenic
AATATGCAATTCAACGACCG+CGG 0.741788 1_2:+52810773 None:intergenic
AGACCTGACGAGCTTCATGG+CGG 0.747768 1_2:+52814331 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!!! AATATTTTAAAATTCAAGAA+GGG + chr1_2:52810979-52810998 None:intergenic 10.0%
!!! TAATATTTTAAAATTCAAGA+AGG + chr1_2:52810980-52810999 None:intergenic 10.0%
!! ATAAATTACAAAGTATAAAA+TGG + chr1_2:52811097-52811116 None:intergenic 10.0%
!!! TATTTTTTAAAACCATTTAT+CGG - chr1_2:52812179-52812198 Msa0076630:intron 10.0%
!!! TTTATTTATTTTAAAGTTCA+AGG - chr1_2:52812528-52812547 Msa0076630:intron 10.0%
!!! ATGAAAAAAATTAATTTGAA+TGG - chr1_2:52812628-52812647 Msa0076630:intron 10.0%
!! ATGAATACAATCTTATTATA+TGG + chr1_2:52811886-52811905 None:intergenic 15.0%
!!! GATAAATGGTTTTAAAAAAT+AGG + chr1_2:52812180-52812199 None:intergenic 15.0%
!! AAAGACTTAGATATATTAAA+TGG + chr1_2:52813696-52813715 None:intergenic 15.0%
!!! ATATCTAAGTCTTTTAATTT+TGG - chr1_2:52813702-52813721 Msa0076630:intron 15.0%
!!! TATCTAAGTCTTTTAATTTT+GGG - chr1_2:52813703-52813722 Msa0076630:intron 15.0%
!!! GAAAGTTTTTCTTTGAAAAT+CGG - chr1_2:52810658-52810677 Msa0076630:three_prime_UTR 20.0%
!!! TTTTTTGTTGTTGTTGAAAT+AGG - chr1_2:52811005-52811024 Msa0076630:CDS 20.0%
!! TCAAAAATTGTATTGAAAGA+GGG + chr1_2:52811639-52811658 None:intergenic 20.0%
!!! TCTTTCAATACAATTTTTGA+GGG - chr1_2:52811639-52811658 Msa0076630:intron 20.0%
!!! TATGTGTTTTCTTAATTTGT+TGG - chr1_2:52812450-52812469 Msa0076630:intron 20.0%
!! GAATATATAATACTTCTCTT+AGG + chr1_2:52813069-52813088 None:intergenic 20.0%
!!! AACGTTTTAATTTCATTTTC+AGG - chr1_2:52814054-52814073 Msa0076630:intron 20.0%
!!! ACGTTTTAATTTCATTTTCA+GGG - chr1_2:52814055-52814074 Msa0076630:intron 20.0%
!!! TGTTTTTATCATCTTAACTA+AGG - chr1_2:52814384-52814403 Msa0076630:five_prime_UTR 20.0%
!!! GTTTTTATCATCTTAACTAA+GGG - chr1_2:52814385-52814404 Msa0076630:five_prime_UTR 20.0%
!! AATGGATCTTATAATCAATT+AGG - chr1_2:52814409-52814428 Msa0076630:five_prime_UTR 20.0%
!!! TGCACAAGTGTTTTATTTTT+AGG + chr1_2:52810467-52810486 None:intergenic 25.0%
!!! AAACAAGAAGTTGATAGAAA+CGG + chr1_2:52810612-52810631 None:intergenic 25.0%
! GATGTATAATAACAGTAATG+AGG + chr1_2:52810789-52810808 None:intergenic 25.0%
!! GTTATTGTTATTATTCCTCA+TGG - chr1_2:52810886-52810905 Msa0076630:intron 25.0%
! CAGAACAACATCATATTATA+TGG + chr1_2:52811337-52811356 None:intergenic 25.0%
! GTATTATAACAGCATAGATT+AGG - chr1_2:52811594-52811613 Msa0076630:intron 25.0%
! CTCAAAAATTGTATTGAAAG+AGG + chr1_2:52811640-52811659 None:intergenic 25.0%
!! CTCTTTCAATACAATTTTTG+AGG - chr1_2:52811638-52811657 Msa0076630:intron 25.0%
!!! CTTTCAATACAATTTTTGAG+GGG - chr1_2:52811640-52811659 Msa0076630:intron 25.0%
! TGATATACATCCATTGAATT+AGG + chr1_2:52811840-52811859 None:intergenic 25.0%
! TATCTTGCCAAATTGTAATA+GGG + chr1_2:52812078-52812097 None:intergenic 25.0%
! TAGAATGTTAGAAGAAGAAA+GGG + chr1_2:52812260-52812279 None:intergenic 25.0%
! ATAGAATGTTAGAAGAAGAA+AGG + chr1_2:52812261-52812280 None:intergenic 25.0%
!!! TGTTTTCTTAATTTGTTGGT+TGG - chr1_2:52812454-52812473 Msa0076630:intron 25.0%
! AAAACTAAAATCACGAAAAC+GGG + chr1_2:52813221-52813240 None:intergenic 25.0%
! GAAAACTAAAATCACGAAAA+CGG + chr1_2:52813222-52813241 None:intergenic 25.0%
! CTAAAAAAATGGCTTAGATT+AGG + chr1_2:52813269-52813288 None:intergenic 25.0%
!! TTGGTGTGTTTCTAAAAAAA+TGG + chr1_2:52813280-52813299 None:intergenic 25.0%
! AACAAAGTAAAACTAAATCC+AGG + chr1_2:52813367-52813386 None:intergenic 25.0%
!!! GGATTTAGTTTTACTTTGTT+AGG - chr1_2:52813367-52813386 Msa0076630:intron 25.0%
!! AACGCAATTACTTTCTTTTA+TGG - chr1_2:52813507-52813526 Msa0076630:intron 25.0%
! TGCTTGACTAGTATTATATT+TGG + chr1_2:52813595-52813614 None:intergenic 25.0%
! AGTTAATAGTAAAACCATAC+TGG - chr1_2:52813633-52813652 Msa0076630:intron 25.0%
! AAGCACTTGTTCAAATATAA+TGG + chr1_2:52814257-52814276 None:intergenic 25.0%
!!! ACTACTTTGTTGACTTATAA+AGG - chr1_2:52814320-52814339 Msa0076630:intron 25.0%
!! TTTTCTTTGAAAATCGGAAC+AGG - chr1_2:52810664-52810683 Msa0076630:three_prime_UTR 30.0%
CAGTAATGAGGAAGAAAAAA+GGG + chr1_2:52810777-52810796 None:intergenic 30.0%
ACAGTAATGAGGAAGAAAAA+AGG + chr1_2:52810778-52810797 None:intergenic 30.0%
!!! AAAGGGTTTTTAGGGTTTAA+CGG + chr1_2:52810825-52810844 None:intergenic 30.0%
! AATGTAGTGATTTTCTTCTC+CGG - chr1_2:52810942-52810961 Msa0076630:CDS 30.0%
AAGAAGGGTTTATTAGATTC+CGG + chr1_2:52810964-52810983 None:intergenic 30.0%
! TAGGTTGCTTAGTTTGAATA+TGG - chr1_2:52811024-52811043 Msa0076630:CDS 30.0%
!!! ATTACACTGTCATTTTTCGA+AGG - chr1_2:52811218-52811237 Msa0076630:intron 30.0%
!!! TTACACTGTCATTTTTCGAA+GGG - chr1_2:52811219-52811238 Msa0076630:intron 30.0%
!!! TGTATTTGTGAGTTGGTTTT+TGG + chr1_2:52811416-52811435 None:intergenic 30.0%
ATAAACACGCTCAAAAAACA+TGG + chr1_2:52811523-52811542 None:intergenic 30.0%
CATAATTTCCATTTCTCTCT+TGG + chr1_2:52811796-52811815 None:intergenic 30.0%
GTTTGTTCCCTATTACAATT+TGG - chr1_2:52812068-52812087 Msa0076630:intron 30.0%
GTATCTTGCCAAATTGTAAT+AGG + chr1_2:52812079-52812098 None:intergenic 30.0%
!!! GTTTTAAAAAATAGGCGATG+TGG + chr1_2:52812172-52812191 None:intergenic 30.0%
! ATCAAAATTTTAGACACCTG+TGG + chr1_2:52812207-52812226 None:intergenic 30.0%
! CAACTGTATCTCTTTTACAT+TGG + chr1_2:52812416-52812435 None:intergenic 30.0%
CAATGTAAAAGAGATACAGT+TGG - chr1_2:52812414-52812433 Msa0076630:intron 30.0%
TAAGAAAACACATATCGTCA+TGG + chr1_2:52812444-52812463 None:intergenic 30.0%
ACTTTCTTAGACCATGTAAT+TGG + chr1_2:52812793-52812812 None:intergenic 30.0%
AATTACATGGTCTAAGAAAG+TGG - chr1_2:52812792-52812811 Msa0076630:intron 30.0%
TCACTCTACATATTAAACTC+TGG - chr1_2:52812878-52812897 Msa0076630:intron 30.0%
TAAAATGCATAGGCTGATAT+GGG + chr1_2:52813046-52813065 None:intergenic 30.0%
TTCTCTTAGGTAAAATGCAT+AGG + chr1_2:52813056-52813075 None:intergenic 30.0%
AAATGTATGAATGATTCGAC+TGG + chr1_2:52813198-52813217 None:intergenic 30.0%
AATGGCTTAGATTAGGTAAA+AGG + chr1_2:52813262-52813281 None:intergenic 30.0%
! AATTTTCTATTCAACACACC+TGG - chr1_2:52813346-52813365 Msa0076630:intron 30.0%
! GCAATTACTTTCTTTTATGG+AGG - chr1_2:52813510-52813529 Msa0076630:intron 30.0%
! GGTTTTACTATTAACTGTCA+AGG + chr1_2:52813629-52813648 None:intergenic 30.0%
AACAGTTAAACTAACACTGA+AGG - chr1_2:52813826-52813845 Msa0076630:intron 30.0%
ACAGTTAAACTAACACTGAA+GGG - chr1_2:52813827-52813846 Msa0076630:intron 30.0%
!! ACTTGCTGCAATTTTTATTC+AGG - chr1_2:52814004-52814023 Msa0076630:intron 30.0%
! TTCAGAATTTTTGTGCCATA+TGG - chr1_2:52814146-52814165 Msa0076630:intron 30.0%
TTCTTGCTGAATAGTTATGT+TGG - chr1_2:52814217-52814236 Msa0076630:intron 30.0%
ATCATCTTAACTAAGGGAAA+TGG - chr1_2:52814391-52814410 Msa0076630:five_prime_UTR 30.0%
GCTGCATGATAAAAATTGTA+GGG - chr1_2:52814446-52814465 Msa0076630:five_prime_UTR 30.0%
AAGAACGGTACAATTGTTCA+TGG - chr1_2:52810729-52810748 Msa0076630:three_prime_UTR 35.0%
! AACTGATAGTTTAAGTGCTG+AGG - chr1_2:52811063-52811082 Msa0076630:CDS 35.0%
!! ACTGATAGTTTAAGTGCTGA+GGG - chr1_2:52811064-52811083 Msa0076630:CDS 35.0%
!!! TTTGTGAGTTGGTTTTTGGA+GGG + chr1_2:52811412-52811431 None:intergenic 35.0%
!!! ATTTGTGAGTTGGTTTTTGG+AGG + chr1_2:52811413-52811432 None:intergenic 35.0%
CTTAGAGTGTATTTGTGAGT+TGG + chr1_2:52811423-52811442 None:intergenic 35.0%
CAACTCACAAATACACTCTA+AGG - chr1_2:52811421-52811440 Msa0076630:intron 35.0%
AGGAAAGGAAAATGGAAGAA+AGG + chr1_2:52811729-52811748 None:intergenic 35.0%
AAGGAAAGAGGAAAGGAAAA+TGG + chr1_2:52811737-52811756 None:intergenic 35.0%
TGGATAAAAGGAAAGAGGAA+AGG + chr1_2:52811744-52811763 None:intergenic 35.0%
!! AGCTTTGGATAAAAGGAAAG+AGG + chr1_2:52811749-52811768 None:intergenic 35.0%
!! AGAGGAAAGCTTTGGATAAA+AGG + chr1_2:52811756-52811775 None:intergenic 35.0%
TTTCCCTCTTCCTAATTCAA+TGG - chr1_2:52811827-52811846 Msa0076630:intron 35.0%
CATCCATTGAATTAGGAAGA+GGG + chr1_2:52811833-52811852 None:intergenic 35.0%
ACATCCATTGAATTAGGAAG+AGG + chr1_2:52811834-52811853 None:intergenic 35.0%
TAATAGGGAACAAACACCAT+TGG + chr1_2:52812063-52812082 None:intergenic 35.0%
! TTTGTGGTAGTGAACGAAAA+TGG - chr1_2:52812310-52812329 Msa0076630:intron 35.0%
! TTGTGGTAGTGAACGAAAAT+GGG - chr1_2:52812311-52812330 Msa0076630:intron 35.0%
TTAACAAGAGAGAGCTTGAA+TGG - chr1_2:52812356-52812375 Msa0076630:intron 35.0%
TAACAAGAGAGAGCTTGAAT+GGG - chr1_2:52812357-52812376 Msa0076630:intron 35.0%
! ATGGATTGCTCTATGAAACA+TGG + chr1_2:52812660-52812679 None:intergenic 35.0%
ATAGAGCAATCCATTATGCA+GGG - chr1_2:52812666-52812685 Msa0076630:intron 35.0%
TTGCAGTTTCAGAGAGATAA+TGG - chr1_2:52812740-52812759 Msa0076630:intron 35.0%
TGCAGTTTCAGAGAGATAAT+GGG - chr1_2:52812741-52812760 Msa0076630:intron 35.0%
CCAATTGTCCAAAAACAACA+AGG + chr1_2:52812972-52812991 None:intergenic 35.0%
!!! CCTTGTTGTTTTTGGACAAT+TGG - chr1_2:52812969-52812988 Msa0076630:intron 35.0%
! TTTTTGGACAATTGGACTGA+AGG - chr1_2:52812977-52812996 Msa0076630:intron 35.0%
AAAATGCATAGGCTGATATG+GGG + chr1_2:52813045-52813064 None:intergenic 35.0%
GTAAAATGCATAGGCTGATA+TGG + chr1_2:52813047-52813066 None:intergenic 35.0%
TGCAATTCGTGAGATTGTTT+CGG + chr1_2:52813091-52813110 None:intergenic 35.0%
ATCTCACGAATTGCAAGAAA+AGG - chr1_2:52813096-52813115 Msa0076630:intron 35.0%
ACGAATTGCAAGAAAAGGAT+AGG - chr1_2:52813101-52813120 Msa0076630:intron 35.0%
TGAGGTTTAATAAGAAGCTG+GGG + chr1_2:52813309-52813328 None:intergenic 35.0%
GTGAGGTTTAATAAGAAGCT+GGG + chr1_2:52813310-52813329 None:intergenic 35.0%
TGTGAGGTTTAATAAGAAGC+TGG + chr1_2:52813311-52813330 None:intergenic 35.0%
TGCTAAAGTTTGTGATGTGT+TGG - chr1_2:52813442-52813461 Msa0076630:intron 35.0%
GCTAAAGTTTGTGATGTGTT+GGG - chr1_2:52813443-52813462 Msa0076630:intron 35.0%
TGACTAACAATTCATGAAGC+TGG + chr1_2:52813480-52813499 None:intergenic 35.0%
TATAATACTAGTCAAGCACC+AGG - chr1_2:52813597-52813616 Msa0076630:intron 35.0%
ATAATACTAGTCAAGCACCA+GGG - chr1_2:52813598-52813617 Msa0076630:intron 35.0%
AATAGTAAAACCATACTGGC+TGG - chr1_2:52813637-52813656 Msa0076630:intron 35.0%
CAGTTAAACTAACACTGAAG+GGG - chr1_2:52813828-52813847 Msa0076630:intron 35.0%
! TAATGTAAAGTACCAGCAGT+TGG + chr1_2:52813980-52813999 None:intergenic 35.0%
! GCTGCAATTTTTATTCAGGT+TGG - chr1_2:52814008-52814027 Msa0076630:intron 35.0%
GTTGAAAACCAAAAACCAGA+TGG + chr1_2:52814037-52814056 None:intergenic 35.0%
CTCAGTTAAAGAACTCAGAA+AGG + chr1_2:52814299-52814318 None:intergenic 35.0%
CGCTGCATGATAAAAATTGT+AGG - chr1_2:52814445-52814464 Msa0076630:five_prime_UTR 35.0%
TGGTTGTTGAAGTCGAAAAC+CGG - chr1_2:52810523-52810542 Msa0076630:three_prime_UTR 40.0%
ATTGTTCATGGCACCATTAC+AGG - chr1_2:52810741-52810760 Msa0076630:three_prime_UTR 40.0%
! TTGAGCGCAAAGGGTTTTTA+GGG + chr1_2:52810833-52810852 None:intergenic 40.0%
! TTTGAGCGCAAAGGGTTTTT+AGG + chr1_2:52810834-52810853 None:intergenic 40.0%
AAGCAATCTTTGAGCGCAAA+GGG + chr1_2:52810842-52810861 None:intergenic 40.0%
TAAGCAATCTTTGAGCGCAA+AGG + chr1_2:52810843-52810862 None:intergenic 40.0%
!! TGTGTTTGCGAGTTGGATTT+TGG - chr1_2:52811242-52811261 Msa0076630:intron 40.0%
!!! TTGTGAGTTGGTTTTTGGAG+GGG + chr1_2:52811411-52811430 None:intergenic 40.0%
AGTTTGGGGAGAGAAGATTA+GGG - chr1_2:52811479-52811498 Msa0076630:intron 40.0%
AACAAACCCCCTATTCAAGT+TGG - chr1_2:52811682-52811701 Msa0076630:intron 40.0%
! ACAAACCCCCTATTCAAGTT+GGG - chr1_2:52811683-52811702 Msa0076630:intron 40.0%
TCTTGGACACTGTTTGTAAG+GGG + chr1_2:52811779-52811798 None:intergenic 40.0%
CTCTTGGACACTGTTTGTAA+GGG + chr1_2:52811780-52811799 None:intergenic 40.0%
TCTCTTGGACACTGTTTGTA+AGG + chr1_2:52811781-52811800 None:intergenic 40.0%
AACAGTGTCCAAGAGAGAAA+TGG - chr1_2:52811785-52811804 Msa0076630:intron 40.0%
! TGTTGAGCGAGTGTTTTACT+AGG + chr1_2:52812143-52812162 None:intergenic 40.0%
!! AAACCATTTATCGGTTCCAC+AGG - chr1_2:52812188-52812207 Msa0076630:intron 40.0%
AAGAAAGGGCAGACAACAAA+AGG + chr1_2:52812246-52812265 None:intergenic 40.0%
ATTCTATGAAGCAAAGCTCG+AGG - chr1_2:52812274-52812293 Msa0076630:intron 40.0%
AGCTTGAATGGGAAAAGAAG+AGG - chr1_2:52812368-52812387 Msa0076630:intron 40.0%
TGAATGGGAAAAGAAGAGGA+TGG - chr1_2:52812372-52812391 Msa0076630:intron 40.0%
CATAGAGCAATCCATTATGC+AGG - chr1_2:52812665-52812684 Msa0076630:intron 40.0%
TAGAGCAATCCATTATGCAG+GGG - chr1_2:52812667-52812686 Msa0076630:intron 40.0%
AATCCATTATGCAGGGGAAA+GGG - chr1_2:52812673-52812692 Msa0076630:intron 40.0%
!! TTCAGAGAGATAATGGGATG+AGG - chr1_2:52812747-52812766 Msa0076630:intron 40.0%
!! TCAGAGAGATAATGGGATGA+GGG - chr1_2:52812748-52812767 Msa0076630:intron 40.0%
TGAACCAGTTGCCAATTACA+TGG - chr1_2:52812779-52812798 Msa0076630:intron 40.0%
TAGACCATGTAATTGGCAAC+TGG + chr1_2:52812786-52812805 None:intergenic 40.0%
ACATGGTCTAAGAAAGTGGT+TGG - chr1_2:52812796-52812815 Msa0076630:intron 40.0%
! TCTGGAGTTTAAGCGTTTCT+TGG - chr1_2:52812896-52812915 Msa0076630:intron 40.0%
CTGGAGTTTAAGCGTTTCTT+GGG - chr1_2:52812897-52812916 Msa0076630:intron 40.0%
!!! GAATCCAGCCTTGTTGTTTT+TGG - chr1_2:52812961-52812980 Msa0076630:intron 40.0%
TTGTCCAAAAACAACAAGGC+TGG + chr1_2:52812968-52812987 None:intergenic 40.0%
AGCGTGTAGTTATGCTTTGA+TGG - chr1_2:52813141-52813160 Msa0076630:intron 40.0%
TCAAAAGCTTAAGCTGTGTG+AGG + chr1_2:52813327-52813346 None:intergenic 40.0%
TAACTGTCAAGGAAAATCCC+TGG + chr1_2:52813618-52813637 None:intergenic 40.0%
ATAGCAACAAGTCAAGACTC+AGG + chr1_2:52813768-52813787 None:intergenic 40.0%
!! TTGACTTGTTGCTATGCTAC+AGG - chr1_2:52813773-52813792 Msa0076630:intron 40.0%
CTTGAATCTTGAGACTTTGC+TGG - chr1_2:52813922-52813941 Msa0076630:intron 40.0%
!! TTCATTTTCAGGGAAGCCTT+TGG - chr1_2:52814065-52814084 Msa0076630:intron 40.0%
!! TCATTTTCAGGGAAGCCTTT+GGG - chr1_2:52814066-52814085 Msa0076630:intron 40.0%
AATATGCAATTCAACGACCG+CGG + chr1_2:52814128-52814147 None:intergenic 40.0%
TTTGTGCCATATGGTTGTGT+TGG - chr1_2:52814155-52814174 Msa0076630:intron 40.0%
TTGTGCCATATGGTTGTGTT+GGG - chr1_2:52814156-52814175 Msa0076630:intron 40.0%
TGTTGGGAGGAATTTATGAG+TGG - chr1_2:52814172-52814191 Msa0076630:CDS 40.0%
! GGAATTTATGAGTGGTCTTG+TGG - chr1_2:52814180-52814199 Msa0076630:CDS 40.0%
GACTTTAGTTGAGACTTGAG+TGG + chr1_2:52814356-52814375 None:intergenic 40.0%
TCGACTTCAACAACCACGAA+GGG + chr1_2:52810519-52810538 None:intergenic 45.0%
TTCGACTTCAACAACCACGA+AGG + chr1_2:52810520-52810539 None:intergenic 45.0%
GTTCTTGAGCTCGATCGATA+CGG + chr1_2:52810715-52810734 None:intergenic 45.0%
! GTATCGATCGAGCTCAAGAA+CGG - chr1_2:52810714-52810733 Msa0076630:three_prime_UTR 45.0%
! GGGTTAGTACGAACCTGTAA+TGG + chr1_2:52810757-52810776 None:intergenic 45.0%
ACATTCACTACTCTGCCATG+AGG + chr1_2:52810904-52810923 None:intergenic 45.0%
GCTATTCGTTGTTGCACAAC+AGG + chr1_2:52811196-52811215 None:intergenic 45.0%
!! GTTTTTGGAGGGGATGACTT+TGG + chr1_2:52811401-52811420 None:intergenic 45.0%
GAGTTTGGGGAGAGAAGATT+AGG - chr1_2:52811478-52811497 Msa0076630:intron 45.0%
GTTTGGGGAGAGAAGATTAG+GGG - chr1_2:52811480-52811499 Msa0076630:intron 45.0%
!! CAAACCCCCTATTCAAGTTG+GGG - chr1_2:52811684-52811703 Msa0076630:intron 45.0%
TTGCCACTCACTCTAAGTTC+AGG - chr1_2:52812026-52812045 Msa0076630:intron 45.0%
AAGCCTGAACTTAGAGTGAG+TGG + chr1_2:52812032-52812051 None:intergenic 45.0%
ACTCACTCTAAGTTCAGGCT+TGG - chr1_2:52812031-52812050 Msa0076630:intron 45.0%
TCAGGCTTGGAATAGTCCAA+TGG - chr1_2:52812044-52812063 Msa0076630:intron 45.0%
ACACCTGTGGAACCGATAAA+TGG + chr1_2:52812194-52812213 None:intergenic 45.0%
! AGAAGAGGATGGTAGTGAGA+TGG - chr1_2:52812383-52812402 Msa0076630:intron 45.0%
CAATCCATTATGCAGGGGAA+AGG - chr1_2:52812672-52812691 Msa0076630:intron 45.0%
!! CAGAGAGATAATGGGATGAG+GGG - chr1_2:52812749-52812768 Msa0076630:intron 45.0%
!! GATAATGGGATGAGGGGAAA+AGG - chr1_2:52812755-52812774 Msa0076630:intron 45.0%
CTGATATGGGGATCAACAAC+TGG + chr1_2:52813033-52813052 None:intergenic 45.0%
TAAGAAGCTGGGGATGAGAT+TGG + chr1_2:52813299-52813318 None:intergenic 45.0%
TGAGATGATCCAGAGTCACT+GGG + chr1_2:52813859-52813878 None:intergenic 45.0%
TCTGGATCATCTCAGTGTGA+GGG - chr1_2:52813865-52813884 Msa0076630:intron 45.0%
CAAGATTCAAGCTGTCAGGA+AGG + chr1_2:52813913-52813932 None:intergenic 45.0%
GTCTCAAGATTCAAGCTGTC+AGG + chr1_2:52813917-52813936 None:intergenic 45.0%
! TGAGACTTTGCTGGTTGAAG+AGG - chr1_2:52813931-52813950 Msa0076630:intron 45.0%
!! CATTTTCAGGGAAGCCTTTG+GGG - chr1_2:52814067-52814086 Msa0076630:intron 45.0%
TTCCTCCCAACACAACCATA+TGG + chr1_2:52814164-52814183 None:intergenic 45.0%
GTTGAGACTTGAGTGGTGTT+AGG + chr1_2:52814349-52814368 None:intergenic 45.0%
ACTTCAACAACCACGAAGGG+AGG + chr1_2:52810516-52810535 None:intergenic 50.0%
GCTCTGTTTGCGAGTTCAGA+GGG - chr1_2:52811443-52811462 Msa0076630:intron 50.0%
CTCTGTTTGCGAGTTCAGAG+GGG - chr1_2:52811444-52811463 Msa0076630:intron 50.0%
CCAACCCCAACTTGAATAGG+GGG + chr1_2:52811691-52811710 None:intergenic 50.0%
CCCAACCCCAACTTGAATAG+GGG + chr1_2:52811692-52811711 None:intergenic 50.0%
!! CCCCTATTCAAGTTGGGGTT+GGG - chr1_2:52811689-52811708 Msa0076630:intron 50.0%
CCCCAACCCCAACTTGAATA+GGG + chr1_2:52811693-52811712 None:intergenic 50.0%
!! CCCTATTCAAGTTGGGGTTG+GGG - chr1_2:52811690-52811709 Msa0076630:intron 50.0%
!! CCTATTCAAGTTGGGGTTGG+GGG - chr1_2:52811691-52811710 Msa0076630:intron 50.0%
!! CTATTCAAGTTGGGGTTGGG+GGG - chr1_2:52811692-52811711 Msa0076630:intron 50.0%
!! TATTCAAGTTGGGGTTGGGG+GGG - chr1_2:52811693-52811712 Msa0076630:intron 50.0%
GTAAGGGGAGAGGAAAGCTT+TGG + chr1_2:52811764-52811783 None:intergenic 50.0%
GACACTGTTTGTAAGGGGAG+AGG + chr1_2:52811774-52811793 None:intergenic 50.0%
CTACCACAAATCGTGAGTCG+CGG + chr1_2:52812300-52812319 None:intergenic 50.0%
GTCTAAGAAAGTGGTTGGCG+TGG - chr1_2:52812801-52812820 Msa0076630:intron 50.0%
GTTTGTGATGTGTTGGGCTC+AGG - chr1_2:52813449-52813468 Msa0076630:intron 50.0%
CTGAGATGATCCAGAGTCAC+TGG + chr1_2:52813860-52813879 None:intergenic 50.0%
CTCTGGATCATCTCAGTGTG+AGG - chr1_2:52813864-52813883 Msa0076630:intron 50.0%
CTGGATCATCTCAGTGTGAG+GGG - chr1_2:52813866-52813885 Msa0076630:intron 50.0%
AAGCCTAAGAAGCCAACTGC+TGG - chr1_2:52813965-52813984 Msa0076630:intron 50.0%
! GTACCAGCAGTTGGCTTCTT+AGG + chr1_2:52813971-52813990 None:intergenic 50.0%
!! TATTCAGGTTGGCTGCCATC+TGG - chr1_2:52814019-52814038 Msa0076630:intron 50.0%
!!! GTTGGCTGCCATCTGGTTTT+TGG - chr1_2:52814026-52814045 Msa0076630:intron 50.0%
TGCCATATGGTTGTGTTGGG+AGG - chr1_2:52814159-52814178 Msa0076630:CDS 50.0%
AACAACCACGAAGGGAGGAG+CGG + chr1_2:52810511-52810530 None:intergenic 55.0%
AGACCTGACGAGCTTCATGG+CGG + chr1_2:52810570-52810589 None:intergenic 55.0%
GAGAGACCTGACGAGCTTCA+TGG + chr1_2:52810573-52810592 None:intergenic 55.0%
! CGAAGGGTGTGTTTGCGAGT+TGG - chr1_2:52811235-52811254 Msa0076630:intron 55.0%
GGCTCTGTTTGCGAGTTCAG+AGG - chr1_2:52811442-52811461 Msa0076630:intron 55.0%
TGTTTGCGAGTTCAGAGGGG+AGG - chr1_2:52811447-52811466 Msa0076630:intron 55.0%
GTTTGCGAGTTCAGAGGGGA+GGG - chr1_2:52811448-52811467 Msa0076630:intron 55.0%
TTTGCGAGTTCAGAGGGGAG+GGG - chr1_2:52811449-52811468 Msa0076630:intron 55.0%
!! CCCCCTATTCAAGTTGGGGT+TGG - chr1_2:52811688-52811707 Msa0076630:intron 55.0%
CCCCCAACCCCAACTTGAAT+AGG + chr1_2:52811694-52811713 None:intergenic 55.0%
!! ATTCAAGTTGGGGTTGGGGG+GGG - chr1_2:52811694-52811713 Msa0076630:intron 55.0%
CCACCCTTTCCCCTGCATAA+TGG + chr1_2:52812679-52812698 None:intergenic 55.0%
CCATTATGCAGGGGAAAGGG+TGG - chr1_2:52812676-52812695 Msa0076630:intron 55.0%
TTATGCAGGGGAAAGGGTGG+TGG - chr1_2:52812679-52812698 Msa0076630:intron 55.0%
TATGCAGGGGAAAGGGTGGT+GGG - chr1_2:52812680-52812699 Msa0076630:intron 55.0%
TGGGTGAGGTTGAGGGATAG+TGG - chr1_2:52812699-52812718 Msa0076630:intron 55.0%
! GTGAGGTTGAGGGATAGTGG+TGG - chr1_2:52812702-52812721 Msa0076630:intron 55.0%
!! GTTGAGGGATAGTGGTGGAC+TGG - chr1_2:52812707-52812726 Msa0076630:intron 55.0%
!! GGATAGTGGTGGACTGGTGA+CGG - chr1_2:52812713-52812732 Msa0076630:intron 55.0%
GCAACATCTGCCAGCCAGTA+TGG + chr1_2:52813650-52813669 None:intergenic 55.0%
GGGGAAAAACCCAGTGACTC+TGG - chr1_2:52813847-52813866 Msa0076630:intron 55.0%
!! TGCTGGTTGAAGAGGCACCT+AGG - chr1_2:52813939-52813958 Msa0076630:intron 55.0%
!! GCTGGTTGAAGAGGCACCTA+GGG - chr1_2:52813940-52813959 Msa0076630:intron 55.0%
GGCTTCTTAGGCTTGACCCT+AGG + chr1_2:52813959-52813978 None:intergenic 55.0%
ACAACCACGAAGGGAGGAGC+GGG + chr1_2:52810510-52810529 None:intergenic 60.0%
ACGAGCTTCATGGCGGCGAT+GGG + chr1_2:52810563-52810582 None:intergenic 60.0%
GAGTTCAGAGGGGAGGGGAA+GGG - chr1_2:52811454-52811473 Msa0076630:intron 60.0%
GGGAGGGGAAGGGAGAGTTT+GGG - chr1_2:52811464-52811483 Msa0076630:intron 60.0%
GGAGGGGAAGGGAGAGTTTG+GGG - chr1_2:52811465-52811484 Msa0076630:intron 60.0%
!! TTCAAGTTGGGGTTGGGGGG+GGG - chr1_2:52811695-52811714 Msa0076630:intron 60.0%
AGGCCGCGACTCACGATTTG+TGG - chr1_2:52812294-52812313 Msa0076630:intron 60.0%
!! AAGGGTGGTGGGTGAGGTTG+AGG - chr1_2:52812691-52812710 Msa0076630:intron 60.0%
!! AGGGTGGTGGGTGAGGTTGA+GGG - chr1_2:52812692-52812711 Msa0076630:intron 60.0%
!! TAGTGGTGGACTGGTGACGG+TGG - chr1_2:52812716-52812735 Msa0076630:intron 60.0%
CGAGCTTCATGGCGGCGATG+GGG + chr1_2:52810562-52810581 None:intergenic 65.0%
GACGAGCTTCATGGCGGCGA+TGG + chr1_2:52810564-52810583 None:intergenic 65.0%
TCGCCGCCATGAAGCTCGTC+AGG - chr1_2:52810564-52810583 Msa0076630:three_prime_UTR 65.0%
CGAGTTCAGAGGGGAGGGGA+AGG - chr1_2:52811453-52811472 Msa0076630:intron 65.0%
GGGGAGGGGAAGGGAGAGTT+TGG - chr1_2:52811463-52811482 Msa0076630:intron 65.0%
!! TCAAGTTGGGGTTGGGGGGG+GGG - chr1_2:52811696-52811715 Msa0076630:intron 65.0%
!! AGGGGAAAGGGTGGTGGGTG+AGG - chr1_2:52812685-52812704 Msa0076630:intron 65.0%
!! TCAGGGAAGCCTTTGGGGCG+TGG - chr1_2:52814072-52814091 Msa0076630:intron 65.0%
!! CAGGGAAGCCTTTGGGGCGT+GGG - chr1_2:52814073-52814092 Msa0076630:intron 65.0%
CTGACCCGCTCCTCCCTTCG+TGG - chr1_2:52810503-52810522 Msa0076630:three_prime_UTR 70.0%
ATGGGGCGGAAGATGGGCGC+CGG + chr1_2:52810545-52810564 None:intergenic 70.0%
GCGGCGATGGGGCGGAAGAT+GGG + chr1_2:52810551-52810570 None:intergenic 70.0%
GCTTCATGGCGGCGATGGGG+CGG + chr1_2:52810559-52810578 None:intergenic 70.0%
! CAAGTTGGGGTTGGGGGGGG+GGG - chr1_2:52811697-52811716 Msa0076630:intron 70.0%
AAGTTGGGGTTGGGGGGGGG+GGG - chr1_2:52811698-52811717 Msa0076630:intron 70.0%
!! AAGCCTTTGGGGCGTGGGCG+CGG - chr1_2:52814078-52814097 Msa0076630:intron 70.0%
GGCGGCGATGGGGCGGAAGA+TGG + chr1_2:52810552-52810571 None:intergenic 75.0%
AGTTGGGGTTGGGGGGGGGG+GGG - chr1_2:52811699-52811718 Msa0076630:intron 75.0%
! CGAGGGCGTGGACGTGGTCG+TGG - chr1_2:52814102-52814121 Msa0076630:intron 75.0%
CGTGGACGTGGTCGTGGCCG+CGG - chr1_2:52814108-52814127 Msa0076630:intron 75.0%
! GTTGGGGTTGGGGGGGGGGG+GGG - chr1_2:52811700-52811719 Msa0076630:intron 80.0%
! CGACCGCGCCCACGCCCCAA+AGG + chr1_2:52814084-52814103 None:intergenic 80.0%
! TTGGGGCGTGGGCGCGGTCG+AGG - chr1_2:52814084-52814103 Msa0076630:intron 80.0%
! TGGGGCGTGGGCGCGGTCGA+GGG - chr1_2:52814085-52814104 Msa0076630:intron 80.0%
!! CGCGGTCGAGGGCGTGGACG+TGG - chr1_2:52814096-52814115 Msa0076630:intron 80.0%
! CGTGGGCGCGGTCGAGGGCG+TGG - chr1_2:52814090-52814109 Msa0076630:intron 85.0%
Chromosome Type Strat End Strand Name
chr1_2 gene 52810439 52814481 52810439 ID=Msa0076630;Name=Msa0076630
chr1_2 mRNA 52810439 52814481 52810439 ID=Msa0076630-mRNA-1;Parent=Msa0076630;Name=Msa0076630-mRNA-1;_AED=0.01;_eAED=0.01;_QI=133|1|1|1|1|1|4|344|114
chr1_2 exon 52810439 52810844 52810439 ID=Msa0076630-mRNA-1:exon:16311;Parent=Msa0076630-mRNA-1
chr1_2 exon 52810934 52811125 52810934 ID=Msa0076630-mRNA-1:exon:16310;Parent=Msa0076630-mRNA-1
chr1_2 exon 52814158 52814234 52814158 ID=Msa0076630-mRNA-1:exon:16309;Parent=Msa0076630-mRNA-1
chr1_2 exon 52814335 52814481 52814335 ID=Msa0076630-mRNA-1:exon:16308;Parent=Msa0076630-mRNA-1
chr1_2 five_prime_UTR 52814349 52814481 52814349 ID=Msa0076630-mRNA-1:five_prime_utr;Parent=Msa0076630-mRNA-1
chr1_2 CDS 52814335 52814348 52814335 ID=Msa0076630-mRNA-1:cds;Parent=Msa0076630-mRNA-1
chr1_2 CDS 52814158 52814234 52814158 ID=Msa0076630-mRNA-1:cds;Parent=Msa0076630-mRNA-1
chr1_2 CDS 52810934 52811125 52810934 ID=Msa0076630-mRNA-1:cds;Parent=Msa0076630-mRNA-1
chr1_2 CDS 52810783 52810844 52810783 ID=Msa0076630-mRNA-1:cds;Parent=Msa0076630-mRNA-1
chr1_2 three_prime_UTR 52810439 52810782 52810439 ID=Msa0076630-mRNA-1:three_prime_utr;Parent=Msa0076630-mRNA-1
Gene Sequence

>Msa0076630

ATGAAGCTCGTCAGGTTTCTGATGAAATTGAACAACGAAACCGTATCGATCGAGCTCAAGAACGGTACAATTGTTCATGGCACCATTACAGGTGTTGATATCAGCATGAACACACATTTGAAAACAGTTAAACTAACACTGAAGGGGAAAAACCCAGTGACTCTGGATCATCTCAGTGTGAGGGGTAACAACATTCGTTATTATATCCTTCCTGACAGCTTGAATCTTGAGACTTTGCTGGTTGAAGAGGCACCTAGGGTCAAGCCTAAGAAGCCAACTGCTGGGAAGCCTTTGGGGCGTGGGCGCGGTCGAGGGCGTGGACGTGGTCGTGGCCGCGGTCGTTGA
Protein sequence

>Msa0076630

MKLVRFLMKLNNETVSIELKNGTIVHGTITGVDISMNTHLKTVKLTLKGKNPVTLDHLSVRGNNIRYYILPDSLNLETLLVEEAPRVKPKKPTAGKPLGRGRGRGRGRGRGRGR*