AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa0079440

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0079440 MtrunA17_Chr1g0190651 95.139 144 7 0 8 151 1 144 1.56e-99 283
Msa0079440 MtrunA17_Chr1g0190671 90.972 144 13 0 8 151 1 144 1.63e-94 270
Msa0079440 MtrunA17_Chr1g0190661 84.028 144 23 0 8 151 1 144 4.80e-89 256
Msa0079440 MtrunA17_Chr7g0274181 70.000 120 36 0 36 155 69 188 2.23e-59 183
Msa0079440 MtrunA17_Chr1g0190641 59.124 137 55 1 15 151 4 139 6.22e-57 175
Msa0079440 MtrunA17_Chr6g0484021 52.229 157 54 5 17 156 31 183 1.14e-45 157
Msa0079440 MtrunA17_Chr6g0483971 52.174 138 59 3 17 152 11 143 5.81e-44 147
Msa0079440 MtrunA17_Chr6g0484031 47.518 141 62 3 18 151 10 145 6.18e-37 132
Msa0079440 MtrunA17_Chr6g0484041 48.667 150 57 5 17 151 23 167 9.47e-36 130
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0079440 AT3G04720.1 65.468 139 48 0 31 169 71 209 4.21e-62 191

Find 150 sgRNAs with CRISPR-Local

Find 191 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
TTCCTTGGGTGTTTGGATTT+TGG 0.080535 1_2:-57321350 Msa0079440:three_prime_UTR
AATAAATTAATTGTGGTATT+TGG 0.108349 1_2:+57321286 None:intergenic
TTGCTTGGATTCTAAATAAA+AGG 0.123700 1_2:-57321486 Msa0079440:three_prime_UTR
TATACAAACTTGGTGAAATT+TGG 0.126305 1_2:-57321976 Msa0079440:three_prime_UTR
ACATTACATTTAATATAATT+TGG 0.136403 1_2:+57321688 None:intergenic
GTAAACATAAATATATATTT+TGG 0.152642 1_2:+57322980 None:intergenic
CTATGAATCTATGGAGTTTA+TGG 0.152716 1_2:-57321863 Msa0079440:three_prime_UTR
AAAACATGCTCTGGTGTTTC+TGG 0.158281 1_2:+57322187 None:intergenic
AGATAAAATCTTTCAAATTT+AGG 0.161029 1_2:-57321919 Msa0079440:three_prime_UTR
ACAAGTGCTATATGATTTAT+TGG 0.171995 1_2:-57322580 Msa0079440:three_prime_UTR
TATATTTATGTTTACATAAT+TGG 0.179994 1_2:-57322972 Msa0079440:three_prime_UTR
GGAGTAGCAACTCATAAATT+TGG 0.190200 1_2:-57321839 Msa0079440:three_prime_UTR
GTTAGACCTGCTAGCAATTT+AGG 0.190315 1_2:-57321538 Msa0079440:three_prime_UTR
ACTACAGTATCATGAATAAT+AGG 0.203421 1_2:+57322679 None:intergenic
GGTATATGTGCCTTTGTTTC+GGG 0.209498 1_2:+57323166 None:intergenic
CTACTTCTTCCTTGGGTGTT+TGG 0.210353 1_2:-57321357 Msa0079440:three_prime_UTR
CGGTATATGTGCCTTTGTTT+CGG 0.230641 1_2:+57323165 None:intergenic
CATGCGTTGGTAAACAATTT+CGG 0.256876 1_2:+57322095 None:intergenic
GGTGATGTCATTGTTGAAAT+TGG 0.269592 1_2:-57321407 Msa0079440:three_prime_UTR
GAAACACACAAAAGAGAAAA+TGG 0.283121 1_2:-57324032 Msa0079440:CDS
TTGGTTTCCTACTTCTTCCT+TGG 0.297372 1_2:-57321365 Msa0079440:three_prime_UTR
ATGCGTTGGTAAACAATTTC+GGG 0.297876 1_2:+57322096 None:intergenic
CCTGCAAGCTGCTCTCTCTA+TGG 0.303512 1_2:+57321797 None:intergenic
CAATTGTCCATGGTGTAATA+TGG 0.306310 1_2:-57322212 Msa0079440:three_prime_UTR
TTGTCATGGCGTAGCAAATA+TGG 0.308099 1_2:-57323830 Msa0079440:CDS
ACGCATTAGTAAACAATTTC+CGG 0.325047 1_2:+57322270 None:intergenic
CCCCTAAGAGATAAAATATT+AGG 0.332046 1_2:-57322795 Msa0079440:three_prime_UTR
ACAAAATTTATCACCTTTGA+CGG 0.335398 1_2:+57322129 None:intergenic
TCTCTGCCTGTTGGCCCAGC+TGG 0.347389 1_2:+57323788 None:intergenic
ATAACCCTCAGAATATTAAC+TGG 0.349154 1_2:-57323904 Msa0079440:CDS
GATGAGTTTGGTATGTTTGT+TGG 0.361845 1_2:-57321612 Msa0079440:three_prime_UTR
ACAGAGAACTTTGAGCTTGT+TGG 0.365729 1_2:-57324002 Msa0079440:CDS
CCATAGAGAGAGCAGCTTGC+AGG 0.367426 1_2:-57321797 Msa0079440:three_prime_UTR
TGGTTTCCTACTTCTTCCTT+GGG 0.370222 1_2:-57321364 Msa0079440:three_prime_UTR
TTAATTGTGGTATTTGGAAA+TGG 0.374881 1_2:+57321292 None:intergenic
CGTATCTAAGACCCTAGGTT+AGG 0.376640 1_2:-57323060 Msa0079440:three_prime_UTR
TTGAATCCCTCGACTATTTG+AGG 0.386429 1_2:+57322643 None:intergenic
GAGTAGCAACTCATAAATTT+GGG 0.400397 1_2:-57321838 Msa0079440:three_prime_UTR
ATCTGTCTTCGAGATGAGTT+TGG 0.407014 1_2:-57321624 Msa0079440:three_prime_UTR
TAGGGAAACAATGGGAAAAC+AGG 0.409710 1_2:+57322698 None:intergenic
GTATGTTTGCTATGAATCTA+TGG 0.413185 1_2:-57321872 Msa0079440:three_prime_UTR
AACATACACATATCCTCTTC+AGG 0.420105 1_2:+57321888 None:intergenic
TGTGGACCAGCTGGGCCAAC+AGG 0.421055 1_2:-57323794 Msa0079440:CDS
CACATTACACCAAGGACAAT+TGG 0.421991 1_2:+57322043 None:intergenic
CATATTACACCATGGACAAT+TGG 0.426552 1_2:+57322213 None:intergenic
TATCAAAACAACGCGGGGAT+TGG 0.432261 1_2:-57321648 Msa0079440:three_prime_UTR
GTCAGAGTGCAAACAACGTT+AGG 0.433268 1_2:-57323943 Msa0079440:CDS
ATAAACGTCATGAATTGAAT+AGG 0.433700 1_2:-57321428 Msa0079440:three_prime_UTR
CTACAGTATCATGAATAATA+GGG 0.436676 1_2:+57322680 None:intergenic
CTTGGTGAAATTTGGATTTG+TGG 0.437981 1_2:-57321968 Msa0079440:three_prime_UTR
GTTAGTGTACATAGGTATAC+TGG 0.444259 1_2:+57322433 None:intergenic
TGCAGCAGGCCAGGTTGTAT+TGG 0.447724 1_2:-57321720 Msa0079440:three_prime_UTR
TGCTTGGATTCTAAATAAAA+GGG 0.449179 1_2:-57321485 Msa0079440:three_prime_UTR
CTTAATCCACTTGCAAAATG+AGG 0.449961 1_2:-57322519 Msa0079440:three_prime_UTR
CACTGCAGTAAAACATGCTC+TGG 0.450407 1_2:+57322178 None:intergenic
CCAGTGTATACTGTGCTACT+TGG 0.451011 1_2:-57323868 Msa0079440:CDS
TTAACTGAGAAGTGCATGTT+GGG 0.451185 1_2:-57322067 Msa0079440:three_prime_UTR
GTATCTAAGACCCTAGGTTA+GGG 0.452442 1_2:-57323059 Msa0079440:three_prime_UTR
TTAACCAACGTGGCTGCTCT+TGG 0.455627 1_2:-57321384 Msa0079440:three_prime_UTR
GATTCTAAATAAAAGGGTTG+TGG 0.462410 1_2:-57321479 Msa0079440:three_prime_UTR
TTAGGAGTGGATGGGAGAGC+TGG 0.462735 1_2:-57321520 Msa0079440:three_prime_UTR
TAACTGTAAGGTGACCCTTC+TGG 0.462881 1_2:+57323364 None:intergenic
TCAATTCACGTTGACTTATG+TGG 0.467528 1_2:+57322459 None:intergenic
TCATTCTAATCTTTAGCACT+TGG 0.467960 1_2:-57322852 Msa0079440:three_prime_UTR
TCAAATTGATACCAATGGAC+AGG 0.479120 1_2:-57323391 Msa0079440:CDS
ATCAGAACAGCATGTGCAGC+AGG 0.485363 1_2:-57321734 Msa0079440:three_prime_UTR
CAAAGCTATAGTTAACTGTA+AGG 0.486461 1_2:+57323352 None:intergenic
AACAGCATGTGCAGCAGGCC+AGG 0.486850 1_2:-57321729 Msa0079440:three_prime_UTR
CCGATATCAACTGTCAGAAC+CGG 0.487353 1_2:+57321567 None:intergenic
ATGAATAATAGGGAAACAAT+GGG 0.488502 1_2:+57322690 None:intergenic
ATCTAAGTCCAGTCCACCAT+TGG 0.492671 1_2:+57323428 None:intergenic
TAAAACTGTGCAGTTGCATC+CGG 0.493067 1_2:-57321586 Msa0079440:three_prime_UTR
GAATACTGCAACTGGTGCTC+AGG 0.493434 1_2:-57323478 Msa0079440:CDS
AGAGCAGCTTGCAGGCAGAA+TGG 0.495968 1_2:-57321789 Msa0079440:three_prime_UTR
TGCTACGCCATGACAAGGGC+TGG 0.498360 1_2:+57323837 None:intergenic
CATGAATAATAGGGAAACAA+TGG 0.499447 1_2:+57322689 None:intergenic
GCTAGCAATTTAGGAGTGGA+TGG 0.502710 1_2:-57321529 Msa0079440:three_prime_UTR
CCAGTGCTCCAATGGTGGAC+TGG 0.503700 1_2:-57323436 Msa0079440:CDS
CCAGTCCACCATTGGAGCAC+TGG 0.503905 1_2:+57323436 None:intergenic
CCGGTTCTGACAGTTGATAT+CGG 0.506024 1_2:-57321567 Msa0079440:three_prime_UTR
ATTAACTAATAAAGAAGTGC+AGG 0.507677 1_2:-57322242 Msa0079440:three_prime_UTR
AATGTAGCAATCAGCTCAAA+CGG 0.513795 1_2:-57323233 Msa0079440:three_prime_UTR
TTAAGTAAGTTAGTGTACAT+AGG 0.519776 1_2:+57322425 None:intergenic
CTAATAAAGAAGTGCAGGAT+GGG 0.523391 1_2:-57322237 Msa0079440:three_prime_UTR
CAGGTGACGAATACTGCAAC+TGG 0.524837 1_2:-57323486 Msa0079440:CDS
CCGCAAGAATCTCTGCCTGT+TGG 0.525418 1_2:+57323779 None:intergenic
TGGCCCAGCTGGTCCACAAA+AGG 0.525504 1_2:+57323799 None:intergenic
CAGTGTATACTGTGCTACTT+GGG 0.526139 1_2:-57323867 Msa0079440:CDS
ATGATTTCGCACTTGTACGC+AGG 0.527382 1_2:+57323124 None:intergenic
TTAGGATGTTCTTCCTGAAG+AGG 0.527805 1_2:-57321901 Msa0079440:three_prime_UTR
TCCTTGGTGTAATGTGGAGC+TGG 0.536230 1_2:-57322036 Msa0079440:three_prime_UTR
GTGATTTATTGACTCATCAC+TGG 0.538887 1_2:-57323317 Msa0079440:CDS
CAAACACCCAAGGAAGAAGT+AGG 0.540651 1_2:+57321358 None:intergenic
TTCAATCAAATTGATACCAA+TGG 0.541175 1_2:-57323396 Msa0079440:CDS
ATATGGAAATAAATTAATTG+TGG 0.543231 1_2:+57321279 None:intergenic
CTTCTGGTAACCCTGTCCAT+TGG 0.545362 1_2:+57323380 None:intergenic
ATGTTGGGACCAATTGTCCT+TGG 0.553024 1_2:-57322052 Msa0079440:three_prime_UTR
TAATCCCAGTTAATATTCTG+AGG 0.556889 1_2:+57323899 None:intergenic
ATCTTCCACTAACATTGTAC+AGG 0.557897 1_2:+57322319 None:intergenic
ACTAATAAAGAAGTGCAGGA+TGG 0.559173 1_2:-57322238 Msa0079440:three_prime_UTR
ATTAACTGAGAAGTGCATGT+TGG 0.560742 1_2:-57322068 Msa0079440:three_prime_UTR
AATGGACAGGGTTACCAGAA+GGG 0.563174 1_2:-57323378 Msa0079440:CDS
TCCACTCCTAAATTGCTAGC+AGG 0.564055 1_2:+57321532 None:intergenic
AAGACTTGCAGTGCCGTCAA+AGG 0.564665 1_2:-57322142 Msa0079440:three_prime_UTR
TTACATTTAATATAATTTGG+AGG 0.566301 1_2:+57321691 None:intergenic
TTAATCCACTTGCAAAATGA+GGG 0.566597 1_2:-57322518 Msa0079440:three_prime_UTR
TCAGAGTGCAAACAACGTTA+GGG 0.570249 1_2:-57323942 Msa0079440:CDS
ATAGTGGACCAGTGCTCCAA+TGG 0.570594 1_2:-57323444 Msa0079440:CDS
TGCAACTGGTGCTCAGGTAA+CGG 0.577038 1_2:-57323472 Msa0079440:CDS
TATAGCTTTGTTAATTGCGA+CGG 0.577389 1_2:-57323339 Msa0079440:CDS
ATGCAAACCAGCCCTTGTCA+TGG 0.583448 1_2:-57323844 Msa0079440:CDS
ATATTTGCTACGCCATGACA+AGG 0.584472 1_2:+57323832 None:intergenic
AGGAAGTTGCCAATACAACC+TGG 0.588413 1_2:+57321711 None:intergenic
ACCTGCTAGCAATTTAGGAG+TGG 0.589796 1_2:-57321533 Msa0079440:three_prime_UTR
CAATTGTCCTTGGTGTAATG+TGG 0.590297 1_2:-57322042 Msa0079440:three_prime_UTR
CAAATTGATACCAATGGACA+GGG 0.591064 1_2:-57323390 Msa0079440:CDS
GTAATAATAGTATACAAACT+TGG 0.596249 1_2:-57321986 Msa0079440:three_prime_UTR
CTAGCAATTTAGGAGTGGAT+GGG 0.603978 1_2:-57321528 Msa0079440:three_prime_UTR
CATGGCGTAGCAAATATGGT+TGG 0.604474 1_2:-57323826 Msa0079440:CDS
ATTTATCCTCAAATAGTCGA+GGG 0.606876 1_2:-57322649 Msa0079440:three_prime_UTR
GCAGCTTGCAGGCAGAATGG+CGG 0.609274 1_2:-57321786 Msa0079440:three_prime_UTR
TCAGGTAACGGTGAGAATAG+TGG 0.610066 1_2:-57323460 Msa0079440:CDS
TGTGTTTCATAGTAGAAGCA+TGG 0.611414 1_2:+57324047 None:intergenic
CAATGGACAGGGTTACCAGA+AGG 0.617429 1_2:-57323379 Msa0079440:CDS
ATCTAAGACCCTAGGTTAGG+GGG 0.617950 1_2:-57323057 Msa0079440:three_prime_UTR
TTAAAAGAAACCCGAAACAA+AGG 0.621655 1_2:-57323176 Msa0079440:three_prime_UTR
TAGGGTCTTAGATACGCCGC+TGG 0.623707 1_2:+57323067 None:intergenic
GGTGCACAAATTTATTGACA+TGG 0.623953 1_2:+57324068 None:intergenic
GTGGACCAGTGCTCCAATGG+TGG 0.625188 1_2:-57323441 Msa0079440:CDS
AATCCCAGTTAATATTCTGA+GGG 0.631760 1_2:+57323900 None:intergenic
TATTTATCCTCAAATAGTCG+AGG 0.631801 1_2:-57322650 Msa0079440:three_prime_UTR
AGCCAAAATCCAAACACCCA+AGG 0.632407 1_2:+57321348 None:intergenic
TTTGAGTATCAAAACAACGC+GGG 0.633781 1_2:-57321654 Msa0079440:three_prime_UTR
TAACCCTCAGAATATTAACT+GGG 0.635981 1_2:-57323903 Msa0079440:CDS
TTGAGTATCAAAACAACGCG+GGG 0.637154 1_2:-57321653 Msa0079440:three_prime_UTR
TGAATCTATGGAGTTTATGG+AGG 0.638039 1_2:-57321860 Msa0079440:three_prime_UTR
TATCTAAGACCCTAGGTTAG+GGG 0.645324 1_2:-57323058 Msa0079440:three_prime_UTR
ATGTGAACATTCACGTCCAG+CGG 0.654233 1_2:-57323083 Msa0079440:three_prime_UTR
AGGATGGGACCAATTGTCCA+TGG 0.656111 1_2:-57322222 Msa0079440:three_prime_UTR
GTGGGAACGATGTTAGTGAA+TGG 0.656794 1_2:-57323965 Msa0079440:CDS
CCAAGTAGCACAGTATACAC+TGG 0.657659 1_2:+57323868 None:intergenic
AGCGGCGTATCTAAGACCCT+AGG 0.659664 1_2:-57323065 Msa0079440:three_prime_UTR
TATTTGCTACGCCATGACAA+GGG 0.664421 1_2:+57323833 None:intergenic
CCAACAGGCAGAGATTCTTG+CGG 0.667208 1_2:-57323779 Msa0079440:CDS
TCACTAACATCGTTCCCACA+AGG 0.670804 1_2:+57323969 None:intergenic
TCCAGCTCCACATTACACCA+AGG 0.686873 1_2:+57322035 None:intergenic
TCTGGCTCCATATTACACCA+TGG 0.697603 1_2:+57322205 None:intergenic
GAAACCAAGAGCAGCCACGT+TGG 0.705793 1_2:+57321380 None:intergenic
TTGAAATTGGTTAACCAACG+TGG 0.722520 1_2:-57321394 Msa0079440:three_prime_UTR
GGTTCACTAGATAGTGCATG+CGG 0.724459 1_2:+57323145 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!!! GAAAAAATTTAAAGTTATAT+AGG + chr1_2:57322339-57322358 None:intergenic 10.0%
!!! GTAAACATAAATATATATTT+TGG + chr1_2:57322378-57322397 None:intergenic 10.0%
!! TATATTTATGTTTACATAAT+TGG - chr1_2:57322383-57322402 Msa0079440:three_prime_UTR 10.0%
!! ACATTACATTTAATATAATT+TGG + chr1_2:57323670-57323689 None:intergenic 10.0%
!!! AACATAAATATATATTTTGG+AGG + chr1_2:57322375-57322394 None:intergenic 15.0%
!!! AATTTGAAAGATTTTATCTT+CGG + chr1_2:57323435-57323454 None:intergenic 15.0%
!! AGATAAAATCTTTCAAATTT+AGG - chr1_2:57323436-57323455 Msa0079440:CDS 15.0%
!! TTACATTTAATATAATTTGG+AGG + chr1_2:57323667-57323686 None:intergenic 15.0%
!! AATAAATTAATTGTGGTATT+TGG + chr1_2:57324072-57324091 None:intergenic 15.0%
!! TTCAATTAATGATAGTATTG+TGG - chr1_2:57321831-57321850 Msa0079440:three_prime_UTR 20.0%
!!! TGCTACATTAGTTTTATTTA+CGG + chr1_2:57322111-57322130 None:intergenic 20.0%
!!! TCCTAATATTTTATCTCTTA+GGG + chr1_2:57322564-57322583 None:intergenic 20.0%
!!! TTCCTAATATTTTATCTCTT+AGG + chr1_2:57322565-57322584 None:intergenic 20.0%
!!! TATTTATTTTGTCTAAAGAG+TGG - chr1_2:57323062-57323081 Msa0079440:three_prime_UTR 20.0%
!!! AAAGGTGATAAATTTTGTAT+GGG - chr1_2:57323231-57323250 Msa0079440:three_prime_UTR 20.0%
!! GTAATAATAGTATACAAACT+TGG - chr1_2:57323369-57323388 Msa0079440:CDS 20.0%
!!! TTTTTCTAAAATGAGAGAAG+TGG + chr1_2:57321621-57321640 None:intergenic 25.0%
!! TCACTAGATTTCTTCATTTT+GGG - chr1_2:57321717-57321736 Msa0079440:three_prime_UTR 25.0%
! TTCAATCAAATTGATACCAA+TGG - chr1_2:57321959-57321978 Msa0079440:three_prime_UTR 25.0%
!!! CTACATTAGTTTTATTTACG+GGG + chr1_2:57322109-57322128 None:intergenic 25.0%
!!! GCTACATTAGTTTTATTTAC+GGG + chr1_2:57322110-57322129 None:intergenic 25.0%
!!! TGCTTCATTTTTAATATCTC+TGG - chr1_2:57322435-57322454 Msa0079440:three_prime_UTR 25.0%
!! CCTAATATTTTATCTCTTAG+GGG + chr1_2:57322563-57322582 None:intergenic 25.0%
! ATGAATAATAGGGAAACAAT+GGG + chr1_2:57322668-57322687 None:intergenic 25.0%
! CTACAGTATCATGAATAATA+GGG + chr1_2:57322678-57322697 None:intergenic 25.0%
! ACTACAGTATCATGAATAAT+AGG + chr1_2:57322679-57322698 None:intergenic 25.0%
! ACAAGTGCTATATGATTTAT+TGG - chr1_2:57322775-57322794 Msa0079440:three_prime_UTR 25.0%
! TTAAGTAAGTTAGTGTACAT+AGG + chr1_2:57322933-57322952 None:intergenic 25.0%
!! ATTAACTAATAAAGAAGTGC+AGG - chr1_2:57323113-57323132 Msa0079440:three_prime_UTR 25.0%
! ACAAAATTTATCACCTTTGA+CGG + chr1_2:57323229-57323248 None:intergenic 25.0%
!! CAAAGGTGATAAATTTTGTA+TGG - chr1_2:57323230-57323249 Msa0079440:three_prime_UTR 25.0%
!! TATACAAACTTGGTGAAATT+TGG - chr1_2:57323379-57323398 Msa0079440:CDS 25.0%
! TTGCTTGGATTCTAAATAAA+AGG - chr1_2:57323869-57323888 Msa0079440:CDS 25.0%
! TGCTTGGATTCTAAATAAAA+GGG - chr1_2:57323870-57323889 Msa0079440:CDS 25.0%
! ATAAACGTCATGAATTGAAT+AGG - chr1_2:57323927-57323946 Msa0079440:CDS 25.0%
! TTAATTGTGGTATTTGGAAA+TGG + chr1_2:57324066-57324085 None:intergenic 25.0%
GAAACACACAAAAGAGAAAA+TGG - chr1_2:57321323-57321342 Msa0079440:three_prime_UTR 30.0%
ATAACCCTCAGAATATTAAC+TGG - chr1_2:57321451-57321470 Msa0079440:three_prime_UTR 30.0%
TAACCCTCAGAATATTAACT+GGG - chr1_2:57321452-57321471 Msa0079440:three_prime_UTR 30.0%
AATCCCAGTTAATATTCTGA+GGG + chr1_2:57321458-57321477 None:intergenic 30.0%
TAATCCCAGTTAATATTCTG+AGG + chr1_2:57321459-57321478 None:intergenic 30.0%
! CTCACTAGATTTCTTCATTT+TGG - chr1_2:57321716-57321735 Msa0079440:three_prime_UTR 30.0%
! CACTAGATTTCTTCATTTTG+GGG - chr1_2:57321718-57321737 Msa0079440:three_prime_UTR 30.0%
! ATTCTCTTTGACTAGCTAAT+TGG + chr1_2:57321792-57321811 None:intergenic 30.0%
CAAAGCTATAGTTAACTGTA+AGG + chr1_2:57322006-57322025 None:intergenic 30.0%
!! TATAGCTTTGTTAATTGCGA+CGG - chr1_2:57322016-57322035 Msa0079440:three_prime_UTR 30.0%
GAGTACAACTACAACAAAAA+TGG - chr1_2:57322155-57322174 Msa0079440:three_prime_UTR 30.0%
TTAAAAGAAACCCGAAACAA+AGG - chr1_2:57322179-57322198 Msa0079440:three_prime_UTR 30.0%
TCATTCTAATCTTTAGCACT+TGG - chr1_2:57322503-57322522 Msa0079440:three_prime_UTR 30.0%
CCCCTAAGAGATAAAATATT+AGG - chr1_2:57322560-57322579 Msa0079440:three_prime_UTR 30.0%
CATGAATAATAGGGAAACAA+TGG + chr1_2:57322669-57322688 None:intergenic 30.0%
TATTTATCCTCAAATAGTCG+AGG - chr1_2:57322705-57322724 Msa0079440:three_prime_UTR 30.0%
ATTTATCCTCAAATAGTCGA+GGG - chr1_2:57322706-57322725 Msa0079440:three_prime_UTR 30.0%
TTAATCCACTTGCAAAATGA+GGG - chr1_2:57322837-57322856 Msa0079440:three_prime_UTR 30.0%
! ATTTTCCTGTACAATGTTAG+TGG - chr1_2:57323031-57323050 Msa0079440:three_prime_UTR 30.0%
ACGCATTAGTAAACAATTTC+CGG + chr1_2:57323088-57323107 None:intergenic 30.0%
! TGATAAATTTTGTATGGGAG+TGG - chr1_2:57323236-57323255 Msa0079440:three_prime_UTR 30.0%
! TCAGTTAATTTTACATGCGT+TGG + chr1_2:57323276-57323295 None:intergenic 30.0%
!! GTATGTTTGCTATGAATCTA+TGG - chr1_2:57323483-57323502 Msa0079440:CDS 30.0%
CTATGAATCTATGGAGTTTA+TGG - chr1_2:57323492-57323511 Msa0079440:intron 30.0%
GAGTAGCAACTCATAAATTT+GGG - chr1_2:57323517-57323536 Msa0079440:intron 30.0%
!! TTTTGAGTATCAAAACAACG+CGG - chr1_2:57323700-57323719 Msa0079440:intron 30.0%
GATTCTAAATAAAAGGGTTG+TGG - chr1_2:57323876-57323895 Msa0079440:CDS 30.0%
GGTGCACAAATTTATTGACA+TGG + chr1_2:57321290-57321309 None:intergenic 35.0%
TGTGTTTCATAGTAGAAGCA+TGG + chr1_2:57321311-57321330 None:intergenic 35.0%
!!! TTGGTTTTGTGTTTCCTTGT+GGG - chr1_2:57321372-57321391 Msa0079440:three_prime_UTR 35.0%
!! TGTGTTGGTCAGATTTTAGA+GGG + chr1_2:57321694-57321713 None:intergenic 35.0%
!! TTGTGTTGGTCAGATTTTAG+AGG + chr1_2:57321695-57321714 None:intergenic 35.0%
GAAATCTAGTGAGTTTGTGT+TGG + chr1_2:57321709-57321728 None:intergenic 35.0%
TAGCTAGTCAAAGAGAATTG+TGG - chr1_2:57321794-57321813 Msa0079440:three_prime_UTR 35.0%
TCAAATTGATACCAATGGAC+AGG - chr1_2:57321964-57321983 Msa0079440:three_prime_UTR 35.0%
CAAATTGATACCAATGGACA+GGG - chr1_2:57321965-57321984 Msa0079440:three_prime_UTR 35.0%
!! GTGATTTATTGACTCATCAC+TGG - chr1_2:57322038-57322057 Msa0079440:three_prime_UTR 35.0%
AATGTAGCAATCAGCTCAAA+CGG - chr1_2:57322122-57322141 Msa0079440:three_prime_UTR 35.0%
CTTAATCCACTTGCAAAATG+AGG - chr1_2:57322836-57322855 Msa0079440:three_prime_UTR 35.0%
!! TCAATTCACGTTGACTTATG+TGG + chr1_2:57322899-57322918 None:intergenic 35.0%
GTTAGTGTACATAGGTATAC+TGG + chr1_2:57322925-57322944 None:intergenic 35.0%
! GAATTCACTGTATGTTTTGC+AGG - chr1_2:57322964-57322983 Msa0079440:three_prime_UTR 35.0%
ATCTTCCACTAACATTGTAC+AGG + chr1_2:57323039-57323058 None:intergenic 35.0%
! TATTTTGTCTAAAGAGTGGC+CGG - chr1_2:57323066-57323085 Msa0079440:three_prime_UTR 35.0%
! ACTAATAAAGAAGTGCAGGA+TGG - chr1_2:57323117-57323136 Msa0079440:three_prime_UTR 35.0%
! CTAATAAAGAAGTGCAGGAT+GGG - chr1_2:57323118-57323137 Msa0079440:three_prime_UTR 35.0%
CATATTACACCATGGACAAT+TGG + chr1_2:57323145-57323164 None:intergenic 35.0%
CAATTGTCCATGGTGTAATA+TGG - chr1_2:57323143-57323162 Msa0079440:three_prime_UTR 35.0%
! ATGCGTTGGTAAACAATTTC+GGG + chr1_2:57323262-57323281 None:intergenic 35.0%
! CATGCGTTGGTAAACAATTT+CGG + chr1_2:57323263-57323282 None:intergenic 35.0%
! ATTAACTGAGAAGTGCATGT+TGG - chr1_2:57323287-57323306 Msa0079440:CDS 35.0%
! TTAACTGAGAAGTGCATGTT+GGG - chr1_2:57323288-57323307 Msa0079440:CDS 35.0%
CTTGGTGAAATTTGGATTTG+TGG - chr1_2:57323387-57323406 Msa0079440:CDS 35.0%
AACATACACATATCCTCTTC+AGG + chr1_2:57323470-57323489 None:intergenic 35.0%
TGAATCTATGGAGTTTATGG+AGG - chr1_2:57323495-57323514 Msa0079440:intron 35.0%
GGAGTAGCAACTCATAAATT+TGG - chr1_2:57323516-57323535 Msa0079440:intron 35.0%
TTTGAGTATCAAAACAACGC+GGG - chr1_2:57323701-57323720 Msa0079440:intron 35.0%
GATGAGTTTGGTATGTTTGT+TGG - chr1_2:57323743-57323762 Msa0079440:intron 35.0%
GGTGATGTCATTGTTGAAAT+TGG - chr1_2:57323948-57323967 Msa0079440:CDS 35.0%
TTGAAATTGGTTAACCAACG+TGG - chr1_2:57323961-57323980 Msa0079440:CDS 35.0%
ACAGAGAACTTTGAGCTTGT+TGG - chr1_2:57321353-57321372 Msa0079440:three_prime_UTR 40.0%
!!! GTTGGTTTTGTGTTTCCTTG+TGG - chr1_2:57321371-57321390 Msa0079440:three_prime_UTR 40.0%
TCAGAGTGCAAACAACGTTA+GGG - chr1_2:57321413-57321432 Msa0079440:three_prime_UTR 40.0%
!! CAGTGTATACTGTGCTACTT+GGG - chr1_2:57321488-57321507 Msa0079440:three_prime_UTR 40.0%
! TATTTGCTACGCCATGACAA+GGG + chr1_2:57321525-57321544 None:intergenic 40.0%
! ATATTTGCTACGCCATGACA+AGG + chr1_2:57321526-57321545 None:intergenic 40.0%
TTGTCATGGCGTAGCAAATA+TGG - chr1_2:57321525-57321544 Msa0079440:three_prime_UTR 40.0%
! GGTATATGTGCCTTTGTTTC+GGG + chr1_2:57322192-57322211 None:intergenic 40.0%
! CGGTATATGTGCCTTTGTTT+CGG + chr1_2:57322193-57322212 None:intergenic 40.0%
! GTATCTAAGACCCTAGGTTA+GGG - chr1_2:57322296-57322315 Msa0079440:three_prime_UTR 40.0%
! TATCTAAGACCCTAGGTTAG+GGG - chr1_2:57322297-57322316 Msa0079440:three_prime_UTR 40.0%
!! TTTTGGAGGACTAAAGTGTG+AGG + chr1_2:57322361-57322380 None:intergenic 40.0%
TAGGGAAACAATGGGAAAAC+AGG + chr1_2:57322660-57322679 None:intergenic 40.0%
TTGAATCCCTCGACTATTTG+AGG + chr1_2:57322715-57322734 None:intergenic 40.0%
AAAACATGCTCTGGTGTTTC+TGG + chr1_2:57323171-57323190 None:intergenic 40.0%
CACATTACACCAAGGACAAT+TGG + chr1_2:57323315-57323334 None:intergenic 40.0%
! CAATTGTCCTTGGTGTAATG+TGG - chr1_2:57323313-57323332 Msa0079440:CDS 40.0%
TTAGGATGTTCTTCCTGAAG+AGG - chr1_2:57323454-57323473 Msa0079440:CDS 40.0%
TTGAGTATCAAAACAACGCG+GGG - chr1_2:57323702-57323721 Msa0079440:intron 40.0%
ATCTGTCTTCGAGATGAGTT+TGG - chr1_2:57323731-57323750 Msa0079440:intron 40.0%
TAAAACTGTGCAGTTGCATC+CGG - chr1_2:57323769-57323788 Msa0079440:CDS 40.0%
GTTAGACCTGCTAGCAATTT+AGG - chr1_2:57323817-57323836 Msa0079440:CDS 40.0%
CTAGCAATTTAGGAGTGGAT+GGG - chr1_2:57323827-57323846 Msa0079440:CDS 40.0%
! CTGGACTGTTCTATTTTGCT+TGG - chr1_2:57323854-57323873 Msa0079440:CDS 40.0%
TTGGTTTCCTACTTCTTCCT+TGG - chr1_2:57323990-57324009 Msa0079440:CDS 40.0%
TGGTTTCCTACTTCTTCCTT+GGG - chr1_2:57323991-57324010 Msa0079440:CDS 40.0%
! TTCCTTGGGTGTTTGGATTT+TGG - chr1_2:57324005-57324024 Msa0079440:CDS 40.0%
TCACTAACATCGTTCCCACA+AGG + chr1_2:57321389-57321408 None:intergenic 45.0%
GTGGGAACGATGTTAGTGAA+TGG - chr1_2:57321390-57321409 Msa0079440:three_prime_UTR 45.0%
GTCAGAGTGCAAACAACGTT+AGG - chr1_2:57321412-57321431 Msa0079440:three_prime_UTR 45.0%
CCAAGTAGCACAGTATACAC+TGG + chr1_2:57321490-57321509 None:intergenic 45.0%
! CCAGTGTATACTGTGCTACT+TGG - chr1_2:57321487-57321506 Msa0079440:three_prime_UTR 45.0%
CATGGCGTAGCAAATATGGT+TGG - chr1_2:57321529-57321548 Msa0079440:three_prime_UTR 45.0%
! TATGGTTGGACTGCCTTTTG+TGG - chr1_2:57321543-57321562 Msa0079440:three_prime_UTR 45.0%
GTATTCGTCACCTGCATCAT+CGG + chr1_2:57321863-57321882 None:intergenic 45.0%
! TCAGGTAACGGTGAGAATAG+TGG - chr1_2:57321895-57321914 Msa0079440:three_prime_UTR 45.0%
ATCTAAGTCCAGTCCACCAT+TGG + chr1_2:57321930-57321949 None:intergenic 45.0%
AATGGACAGGGTTACCAGAA+GGG - chr1_2:57321977-57321996 Msa0079440:three_prime_UTR 45.0%
TAACTGTAAGGTGACCCTTC+TGG + chr1_2:57321994-57322013 None:intergenic 45.0%
GGTTCACTAGATAGTGCATG+CGG + chr1_2:57322213-57322232 None:intergenic 45.0%
ATGATTTCGCACTTGTACGC+AGG + chr1_2:57322234-57322253 None:intergenic 45.0%
ATGTGAACATTCACGTCCAG+CGG - chr1_2:57322272-57322291 Msa0079440:three_prime_UTR 45.0%
! CGTATCTAAGACCCTAGGTT+AGG - chr1_2:57322295-57322314 Msa0079440:three_prime_UTR 45.0%
! ATCTAAGACCCTAGGTTAGG+GGG - chr1_2:57322298-57322317 Msa0079440:three_prime_UTR 45.0%
! GTATGCCCTCATTTTGCAAG+TGG + chr1_2:57322845-57322864 None:intergenic 45.0%
! TCTGGCTCCATATTACACCA+TGG + chr1_2:57323153-57323172 None:intergenic 45.0%
CACTGCAGTAAAACATGCTC+TGG + chr1_2:57323180-57323199 None:intergenic 45.0%
ATGTTGGGACCAATTGTCCT+TGG - chr1_2:57323303-57323322 Msa0079440:CDS 45.0%
AGGAAGTTGCCAATACAACC+TGG + chr1_2:57323647-57323666 None:intergenic 45.0%
TATCAAAACAACGCGGGGAT+TGG - chr1_2:57323707-57323726 Msa0079440:intron 45.0%
CCGATATCAACTGTCAGAAC+CGG + chr1_2:57323791-57323810 None:intergenic 45.0%
! CCGGTTCTGACAGTTGATAT+CGG - chr1_2:57323788-57323807 Msa0079440:CDS 45.0%
ACCTGCTAGCAATTTAGGAG+TGG - chr1_2:57323822-57323841 Msa0079440:CDS 45.0%
TCCACTCCTAAATTGCTAGC+AGG + chr1_2:57323826-57323845 None:intergenic 45.0%
GCTAGCAATTTAGGAGTGGA+TGG - chr1_2:57323826-57323845 Msa0079440:CDS 45.0%
CAAACACCCAAGGAAGAAGT+AGG + chr1_2:57324000-57324019 None:intergenic 45.0%
CTACTTCTTCCTTGGGTGTT+TGG - chr1_2:57323998-57324017 Msa0079440:CDS 45.0%
AGCCAAAATCCAAACACCCA+AGG + chr1_2:57324010-57324029 None:intergenic 45.0%
ATGCAAACCAGCCCTTGTCA+TGG - chr1_2:57321511-57321530 Msa0079440:three_prime_UTR 50.0%
CCAACAGGCAGAGATTCTTG+CGG - chr1_2:57321576-57321595 Msa0079440:three_prime_UTR 50.0%
CAGGTGACGAATACTGCAAC+TGG - chr1_2:57321869-57321888 Msa0079440:three_prime_UTR 50.0%
GAATACTGCAACTGGTGCTC+AGG - chr1_2:57321877-57321896 Msa0079440:three_prime_UTR 50.0%
TGCAACTGGTGCTCAGGTAA+CGG - chr1_2:57321883-57321902 Msa0079440:three_prime_UTR 50.0%
!! ATAGTGGACCAGTGCTCCAA+TGG - chr1_2:57321911-57321930 Msa0079440:three_prime_UTR 50.0%
CTTCTGGTAACCCTGTCCAT+TGG + chr1_2:57321978-57321997 None:intergenic 50.0%
CAATGGACAGGGTTACCAGA+AGG - chr1_2:57321976-57321995 Msa0079440:three_prime_UTR 50.0%
AGGATGGGACCAATTGTCCA+TGG - chr1_2:57323133-57323152 Msa0079440:three_prime_UTR 50.0%
AAGACTTGCAGTGCCGTCAA+AGG - chr1_2:57323213-57323232 Msa0079440:three_prime_UTR 50.0%
! TCCTTGGTGTAATGTGGAGC+TGG - chr1_2:57323319-57323338 Msa0079440:CDS 50.0%
TCCAGCTCCACATTACACCA+AGG + chr1_2:57323323-57323342 None:intergenic 50.0%
ATCAGAACAGCATGTGCAGC+AGG - chr1_2:57323621-57323640 Msa0079440:intron 50.0%
TTAACCAACGTGGCTGCTCT+TGG - chr1_2:57323971-57323990 Msa0079440:CDS 50.0%
! ACTGCCTTTTGTGGACCAGC+TGG - chr1_2:57321552-57321571 Msa0079440:three_prime_UTR 55.0%
! CTGCCTTTTGTGGACCAGCT+GGG - chr1_2:57321553-57321572 Msa0079440:three_prime_UTR 55.0%
CCGCAAGAATCTCTGCCTGT+TGG + chr1_2:57321579-57321598 None:intergenic 55.0%
! GTGGTGATGACCGATGATGC+AGG - chr1_2:57321850-57321869 Msa0079440:three_prime_UTR 55.0%
TAGGGTCTTAGATACGCCGC+TGG + chr1_2:57322291-57322310 None:intergenic 55.0%
! AGCGGCGTATCTAAGACCCT+AGG - chr1_2:57322290-57322309 Msa0079440:three_prime_UTR 55.0%
AGTTCGCACCCCCTAACCTA+GGG + chr1_2:57322309-57322328 None:intergenic 55.0%
AAGTTCGCACCCCCTAACCT+AGG + chr1_2:57322310-57322329 None:intergenic 55.0%
CCTGCAAGCTGCTCTCTCTA+TGG + chr1_2:57323561-57323580 None:intergenic 55.0%
CCATAGAGAGAGCAGCTTGC+AGG - chr1_2:57323558-57323577 Msa0079440:intron 55.0%
AGAGCAGCTTGCAGGCAGAA+TGG - chr1_2:57323566-57323585 Msa0079440:intron 55.0%
TGCAGCAGGCCAGGTTGTAT+TGG - chr1_2:57323635-57323654 Msa0079440:intron 55.0%
TTAGGAGTGGATGGGAGAGC+TGG - chr1_2:57323835-57323854 Msa0079440:CDS 55.0%
GAAACCAAGAGCAGCCACGT+TGG + chr1_2:57323978-57323997 None:intergenic 55.0%
TGCTACGCCATGACAAGGGC+TGG + chr1_2:57321521-57321540 None:intergenic 60.0%
TGGCCCAGCTGGTCCACAAA+AGG + chr1_2:57321559-57321578 None:intergenic 60.0%
GTGGACCAGTGCTCCAATGG+TGG - chr1_2:57321914-57321933 Msa0079440:three_prime_UTR 60.0%
CCAGTCCACCATTGGAGCAC+TGG + chr1_2:57321922-57321941 None:intergenic 60.0%
!! CCAGTGCTCCAATGGTGGAC+TGG - chr1_2:57321919-57321938 Msa0079440:three_prime_UTR 60.0%
GCAGCTTGCAGGCAGAATGG+CGG - chr1_2:57323569-57323588 Msa0079440:intron 60.0%
AACAGCATGTGCAGCAGGCC+AGG - chr1_2:57323626-57323645 Msa0079440:intron 60.0%
TGTGGACCAGCTGGGCCAAC+AGG - chr1_2:57321561-57321580 Msa0079440:three_prime_UTR 65.0%
TCTCTGCCTGTTGGCCCAGC+TGG + chr1_2:57321570-57321589 None:intergenic 65.0%
Chromosome Type Strat End Strand Name
chr1_2 gene 57321281 57324096 57321281 ID=Msa0079440;Name=Msa0079440
chr1_2 mRNA 57321281 57324096 57321281 ID=Msa0079440-mRNA-1;Parent=Msa0079440;Name=Msa0079440-mRNA-1;_AED=0.03;_eAED=0.03;_QI=40|1|1|1|1|1|2|2000|171
chr1_2 exon 57321281 57323505 57321281 ID=Msa0079440-mRNA-1:exon:18141;Parent=Msa0079440-mRNA-1
chr1_2 exon 57323766 57324096 57323766 ID=Msa0079440-mRNA-1:exon:18140;Parent=Msa0079440-mRNA-1
chr1_2 five_prime_UTR 57324057 57324096 57324057 ID=Msa0079440-mRNA-1:five_prime_utr;Parent=Msa0079440-mRNA-1
chr1_2 CDS 57323766 57324056 57323766 ID=Msa0079440-mRNA-1:cds;Parent=Msa0079440-mRNA-1
chr1_2 CDS 57323281 57323505 57323281 ID=Msa0079440-mRNA-1:cds;Parent=Msa0079440-mRNA-1
chr1_2 three_prime_UTR 57321281 57323280 57321281 ID=Msa0079440-mRNA-1:three_prime_utr;Parent=Msa0079440-mRNA-1
Gene Sequence

>Msa0079440

ATGAAACACACAAAAGAGAAAATGGAGAGCACACAGAGAACTTTGAGCTTGTTGGTTTTGTGTTTCCTTGTGGGAACGATGTTAGTGAATGGTCAGAGTGCAAACAACGTTAGGGCAACATACAATAATTATAACCCTCAGAATATTAACTGGGATTACATTACTGCCAGTGTATACTGTGCTACTTGGGATGCAAACCAGCCCTTGTCATGGCGTAGCAAATATGGTTGGACTGCCTTTTGTGGACCAGCTGGGCCAACAGGCAGAGATTCTTGCGGCAAATGCTTGAGTGTGACGAATACTGCAACTGGTGCTCAGGTAACGGTGAGAATAGTGGACCAGTGCTCCAATGGTGGACTGGACTTAGATGTGAATGTCTTCAATCAAATTGATACCAATGGACAGGGTTACCAGAAGGGTCACCTTACAGTTAACTATAGCTTTGTTAATTGCGACGGTGATTTATTGACTCATCACTGGTGTAATAATTCACTCTTTGTACAAATAAGTAGCTAG
Protein sequence

>Msa0079440

MKHTKEKMESTQRTLSLLVLCFLVGTMLVNGQSANNVRATYNNYNPQNINWDYITASVYCATWDANQPLSWRSKYGWTAFCGPAGPTGRDSCGKCLSVTNTATGAQVTVRIVDQCSNGGLDLDVNVFNQIDTNGQGYQKGHLTVNYSFVNCDGDLLTHHWCNNSLFVQISS*