AlfalfaGEDB | Msa0032290

M. sativa cultivar zhongmu-4 / Msa0032290

KEGG: -
GO: GO:0006950 GO:0006952 GO:0008150 GO:0050896
PFAM: PF00967.20

Nr:

Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score

Swissprot:

Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa0032290	sp\|P32045\|PRP2_SOLLC	69.718	142	41	1	5	144	2	143	8.70e-70	210

Trembl:

Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa0032290	A0A072VN05	95.139	144	7	0	1	144	1	144	1.29e-95	282

TFs/TRs:

Gene ID	Type	Classification

Protein Kinases:

Gene ID	Type	Classification

Network:

Co-expression Network

Gene1	Gene2	correlation coefficient	p_value	FDR
Msa0014790	Msa0032290	0.810123	1.347927e-50	-8.615850e-47
Msa0032290	Msa0050240	0.817595	3.038852e-52	-8.615850e-47
Msa0032290	Msa0063100	0.814706	1.343792e-51	-8.615850e-47
Msa0032290	Msa0079440	0.880020	7.737615e-70	-8.615850e-47
Msa0032290	Msa0104360	0.801525	8.641309e-49	-8.615850e-47
Msa0032290	Msa0151860	0.825353	4.910321e-54	-8.615850e-47
Msa0032290	Msa0153440	0.835084	2.060955e-56	-8.615850e-47
Msa0032290	Msa0172440	0.943240	1.719585e-102	-8.615850e-47
Msa0032290	Msa0180290	0.800197	1.613295e-48	-8.615850e-47
Msa0032290	Msa0193760	0.805596	1.236536e-49	-8.615850e-47
Msa0032290	Msa0212210	0.805944	1.045133e-49	-8.615850e-47
Msa0032290	Msa0232860	0.849950	2.326611e-60	-8.615850e-47
Msa0032290	Msa0235550	0.804288	2.321378e-49	-8.615850e-47
Msa0032290	Msa0254190	0.857697	1.367867e-62	-8.615850e-47
Msa0032290	Msa0272420	0.806538	7.835792e-50	-8.615850e-47
Msa0032290	Msa0274520	0.804266	2.345846e-49	-8.615850e-47
Msa0032290	Msa0363440	0.844661	6.584671e-59	-8.615850e-47
Msa0032290	Msa0389050	0.843451	1.390164e-58	-8.615850e-47
Msa0032290	Msa0410960	0.811552	6.613820e-51	-8.615850e-47
Msa0032290	Msa0439700	0.820250	7.575575e-53	-8.615850e-47
Msa0032290	Msa0455260	0.834271	3.300318e-56	-8.615850e-47
Msa0032290	Msa0457370	0.814864	1.239906e-51	-8.615850e-47
Msa0032290	Msa0481320	0.811742	6.011964e-51	-8.615850e-47
Msa0032290	Msa0533460	0.832808	7.653136e-56	-8.615850e-47
Msa0032290	Msa0589610	0.830698	2.536245e-55	-8.615850e-47
Msa0032290	Msa0599250	0.828627	8.092710e-55	-8.615850e-47
Msa0032290	Msa0630060	0.812345	4.443172e-51	-8.615850e-47
Msa0032290	Msa0632540	0.804742	1.866371e-49	-8.615850e-47
Msa0032290	Msa0678240	0.820502	6.629670e-53	-8.615850e-47
Msa0032290	Msa0718920	0.800713	1.266738e-48	-8.615850e-47
Msa0032290	Msa0729920	0.815039	1.133786e-51	-8.615850e-47
Msa0032290	Msa0733400	0.818917	1.525738e-52	-8.615850e-47
Msa0032290	Msa0739390	0.802959	4.380632e-49	-8.615850e-47
Msa0032290	Msa0752580	0.861602	9.143809e-64	-8.615850e-47
Msa0032290	Msa0754500	0.856406	3.286457e-62	-8.615850e-47
Msa0032290	Msa0756410	0.809628	1.722663e-50	-8.615850e-47
Msa0032290	Msa0770890	0.823375	1.433170e-53	-8.615850e-47
Msa0032290	Msa0811940	0.802525	5.383482e-49	-8.615850e-47
Msa0032290	Msa0817860	0.820206	7.752867e-53	-8.615850e-47
Msa0032290	Msa0818000	0.808152	3.563074e-50	-8.615850e-47
Msa0032290	Msa0819740	0.829239	5.753929e-55	-8.615850e-47
Msa0032290	Msa0830900	0.846094	2.695238e-59	-8.615850e-47
Msa0032290	Msa0842050	0.801321	9.513633e-49	-8.615850e-47
Msa0032290	Msa0867810	0.823264	1.521049e-53	-8.615850e-47
Msa0032290	Msa0883350	0.820099	8.201511e-53	-8.615850e-47
Msa0032290	Msa0883390	0.803577	3.262063e-49	-8.615850e-47
Msa0032290	Msa0883410	0.801732	7.838710e-49	-8.615850e-47
Msa0032290	Msa0883660	0.838331	3.065106e-57	-8.615850e-47
Msa0032290	Msa0883670	0.817020	4.094703e-52	-8.615850e-47
Msa0032290	Msa0892540	0.809321	2.004897e-50	-8.615850e-47
Msa0032290	Msa0919490	0.804234	2.381988e-49	-8.615850e-47
Msa0032290	Msa0957800	0.835367	1.748344e-56	-8.615850e-47
Msa0032290	Msa0984840	0.801727	7.855970e-49	-8.615850e-47
Msa0032290	Msa0986870	0.827008	1.982959e-54	-8.615850e-47
Msa0032290	Msa1006290	0.811712	6.104218e-51	-8.615850e-47
Msa0032290	Msa1006460	0.808704	2.717658e-50	-8.615850e-47
Msa0032290	Msa1006680	0.815778	7.764742e-52	-8.615850e-47
Msa0032290	Msa1009150	0.853560	2.203897e-61	-8.615850e-47
Msa0032290	Msa1009170	0.878666	2.334910e-69	-8.615850e-47
Msa0032290	Msa1009180	0.809932	1.481914e-50	-8.615850e-47
Msa0032290	Msa1009220	0.858147	1.005648e-62	-8.615850e-47
Msa0032290	Msa1033670	0.804540	2.056953e-49	-8.615850e-47
Msa0032290	Msa1037580	0.801536	8.595628e-49	-8.615850e-47
Msa0032290	Msa1050480	0.802581	5.242667e-49	-8.615850e-47
Msa0032290	Msa1050490	0.849944	2.335086e-60	-8.615850e-47
Msa0032290	Msa1050500	0.822019	2.963708e-53	-8.615850e-47
Msa0032290	Msa1052080	0.804689	1.915050e-49	-8.615850e-47
Msa0032290	Msa1098350	0.840707	7.391794e-58	-8.615850e-47
Msa0032290	Msa1098380	0.814092	1.837406e-51	-8.615850e-47
Msa0032290	Msa1107830	0.818607	1.794475e-52	-8.615850e-47
Msa0032290	Msa1135660	0.835313	1.804882e-56	-8.615850e-47
Msa0032290	Msa1138450	0.817620	3.000759e-52	-8.615850e-47
Msa0032290	Msa1138480	0.883496	4.270166e-71	-8.615850e-47
Msa0032290	Msa1138490	0.871855	4.969797e-67	-8.615850e-47
Msa0032290	Msa1140460	0.816835	4.505311e-52	-8.615850e-47
Msa0032290	Msa1151550	0.822700	2.058517e-53	-8.615850e-47
Msa0032290	Msa1182780	0.819934	8.949112e-53	-8.615850e-47
Msa0032290	Msa1182830	0.819598	1.067723e-52	-8.615850e-47
Msa0032290	Msa1195740	0.812926	3.314713e-51	-8.615850e-47
Msa0032290	Msa1199750	0.804100	2.540587e-49	-8.615850e-47
Msa0032290	Msa1204430	0.831327	1.777348e-55	-8.615850e-47
Msa0032290	Msa1232330	0.810927	9.036258e-51	-8.615850e-47
Msa0032290	Msa1238130	0.808547	2.934888e-50	-8.615850e-47
Msa0032290	Msa1246560	0.810579	1.074662e-50	-8.615850e-47
Msa0032290	Msa1247720	0.809191	2.137519e-50	-8.615850e-47
Msa0032290	Msa1247860	0.803466	3.439390e-49	-8.615850e-47
Msa0032290	Msa1272960	0.802594	5.210123e-49	-8.615850e-47
Msa0032290	Msa1275340	0.804063	2.586159e-49	-8.615850e-47
Msa0032290	Msa1279310	0.803533	3.331974e-49	-8.615850e-47
Msa0032290	Msa1279330	0.801344	9.412173e-49	-8.615850e-47
Msa0032290	Msa1279850	0.801776	7.675370e-49	-8.615850e-47
Msa0032290	Msa1279860	0.817476	3.232498e-52	-8.615850e-47
Msa0032290	Msa1279870	0.807863	4.106744e-50	-8.615850e-47
Msa0032290	Msa1285830	0.819952	8.862104e-53	-8.615850e-47
Msa0032290	Msa1296650	0.819593	1.070534e-52	-8.615850e-47
Msa0032290	Msa1311150	0.813125	2.999176e-51	-8.615850e-47
Msa0032290	Msa1311550	0.813062	3.095617e-51	-8.615850e-47
Msa0032290	Msa1317710	0.813641	2.309989e-51	-8.615850e-47
Msa0032290	Msa1328600	0.815823	7.587076e-52	-8.615850e-47
Msa0032290	Msa1340880	0.822364	2.464427e-53	-8.615850e-47
Msa0032290	Msa1352060	0.830797	2.398725e-55	-8.615850e-47
Msa0032290	Msa1386020	0.809077	2.261460e-50	-8.615850e-47
Msa0032290	Msa1396820	0.817393	3.375984e-52	-8.615850e-47
Msa0032290	Msa1412280	0.812138	4.929634e-51	-8.615850e-47
Msa0032290	Msa1426750	0.805779	1.132336e-49	-8.615850e-47
Msa0032290	Msa1459170	0.838796	2.324193e-57	-8.615850e-47

PPI

Gene1	Gene2	Type

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa0032290	MtrunA17_Chr1g0190651	95.139	144	7	0	1	144	1	144	2.48e-99	282
Msa0032290	MtrunA17_Chr1g0190671	89.583	144	15	0	1	144	1	144	3.11e-93	266
Msa0032290	MtrunA17_Chr1g0190661	84.028	144	23	0	1	144	1	144	4.78e-89	256
Msa0032290	MtrunA17_Chr7g0274181	69.167	120	37	0	29	148	69	188	6.77e-59	182
Msa0032290	MtrunA17_Chr1g0190641	58.394	137	56	1	8	144	4	139	1.53e-56	174
Msa0032290	MtrunA17_Chr6g0484021	52.866	157	53	5	10	149	31	183	8.14e-46	157
Msa0032290	MtrunA17_Chr6g0483971	52.899	138	58	3	10	145	11	143	2.10e-44	148
Msa0032290	MtrunA17_Chr6g0484031	46.479	142	62	3	10	144	11	145	5.14e-38	135
Msa0032290	MtrunA17_Chr6g0484041	49.333	150	56	5	10	144	23	167	2.06e-37	135

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa0032290	AT3G04720.1	64.748	139	49	0	24	162	71	209	1.50e-61	189

Find 115 sgRNAs with CRISPR-Local

Find 304 sgRNAs with CRISPR-GE

CRISPR-Local

CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
TATACAAACTTGGTGAAATT+TGG	0.126305	1_1:-59309890	Msa0032290:three_prime_UTR
ACATTACATTTAATATAATT+TGG	0.136403	1_1:+59309600	None:intergenic
GTAAACATAAATATATATTT+TGG	0.152642	1_1:+59310564	None:intergenic
CTATGAATCTATGGAGTTTA+TGG	0.152716	1_1:-59309775	Msa0032290:three_prime_UTR
AAAACATGCTCTGGTGTTTC+TGG	0.158281	1_1:+59310102	None:intergenic
AGATAAAATCTTTCAAATTT+AGG	0.161439	1_1:-59309831	Msa0032290:three_prime_UTR
TATATTTATGTTTACATAAT+TGG	0.179994	1_1:-59310556	Msa0032290:three_prime_UTR
GGAGTAGCAACTCATAAATT+TGG	0.190200	1_1:-59309751	Msa0032290:three_prime_UTR
GGTATATGTGCCTTTGTTTC+GGG	0.209498	1_1:+59310750	None:intergenic
CGGTATATGTGCCTTTGTTT+CGG	0.230641	1_1:+59310749	None:intergenic
CATGCGTCGGTAAACAATTT+CGG	0.273856	1_1:+59310009	None:intergenic
CAATTGTCCATGGTGTAATA+TGG	0.306310	1_1:-59310127	Msa0032290:three_prime_UTR
TTGTCATGGCGTAGCAAATA+TGG	0.308099	1_1:-59311414	Msa0032290:CDS
TGCTGGCAGAATGGCGGACT+GGG	0.313087	1_1:-59309692	Msa0032290:three_prime_UTR
ACGCATTAGTAAACAATTTC+CGG	0.325047	1_1:+59310185	None:intergenic
GAATTCACTGTATGGTTTGC+AGG	0.331345	1_1:-59310307	Msa0032290:three_prime_UTR
ATGCGTCGGTAAACAATTTC+GGG	0.331546	1_1:+59310010	None:intergenic
TCTCTGCCTGTTGGCCCAGC+TGG	0.347389	1_1:+59311372	None:intergenic
ACAAAATTTCTCACCTTTGA+CGG	0.358366	1_1:+59310044	None:intergenic
CCAGCAAGCTGCTCTCTCTA+TGG	0.358450	1_1:+59309709	None:intergenic
ACAGAGAACTTTGAGCTTGT+TGG	0.365729	1_1:-59311586	Msa0032290:CDS
CGTATCTAAGACCCTAGGTT+AGG	0.376640	1_1:-59310644	Msa0032290:three_prime_UTR
CCATAGAGAGAGCAGCTTGC+TGG	0.377636	1_1:-59309709	Msa0032290:three_prime_UTR
ACAACCCTCAGAATATTAAC+TGG	0.383186	1_1:-59311488	Msa0032290:CDS
GTTAGTGTACATAGGTATAC+TGG	0.398676	1_1:+59310350	None:intergenic
GAGTAGCAACTCATAAATTT+GGG	0.400397	1_1:-59309750	Msa0032290:three_prime_UTR
TATATTAAATGTAATGTTGA+AGG	0.404764	1_1:-59309594	Msa0032290:three_prime_UTR
TTGCTGGCAGAATGGCGGAC+TGG	0.405522	1_1:-59309693	Msa0032290:three_prime_UTR
GTATGTTTGCTATGAATCTA+TGG	0.413185	1_1:-59309784	Msa0032290:three_prime_UTR
AACATACACATATCCTCTTC+AGG	0.420105	1_1:+59309800	None:intergenic
TGTGGACCAGCTGGGCCAAC+AGG	0.421055	1_1:-59311378	Msa0032290:CDS
CACATTACACCAAGGACAAT+TGG	0.421991	1_1:+59309957	None:intergenic
CATATTACACCATGGACAAT+TGG	0.426552	1_1:+59310128	None:intergenic
GTCAGAGTGCAAACAACGTT+AGG	0.437077	1_1:-59311527	Msa0032290:CDS
CTTGGTGAAATTTGGATTTG+TGG	0.437981	1_1:-59309882	Msa0032290:three_prime_UTR
TGCAGCAGGCCAGGTTGTAT+TGG	0.447724	1_1:-59309632	Msa0032290:three_prime_UTR
CTTAATCCACTTGCAAAATG+AGG	0.449961	1_1:-59310436	Msa0032290:three_prime_UTR
CACTGCAGTAAAACATGCTC+TGG	0.450407	1_1:+59310093	None:intergenic
CCAGTGTATACTGTGCTACT+TGG	0.451011	1_1:-59311452	Msa0032290:CDS
TTAACTGAGAAGTGCATGTT+GGG	0.451185	1_1:-59309981	Msa0032290:three_prime_UTR
GTATCTAAGACCCTAGGTTA+GGG	0.452442	1_1:-59310643	Msa0032290:three_prime_UTR
TAACTGTAAGGTGACCCTTC+TGG	0.462881	1_1:+59310948	None:intergenic
TATGTATAGAATTCACTGTA+TGG	0.466417	1_1:-59310315	Msa0032290:three_prime_UTR
TCAAATTGATACCAATGGAC+AGG	0.479120	1_1:-59310975	Msa0032290:CDS
GGTGTACAAATTTATTGACT+TGG	0.480575	1_1:+59311654	None:intergenic
ATCAGAACAGCATGTGCAGC+AGG	0.485363	1_1:-59309646	Msa0032290:three_prime_UTR
CAAAGCTATAGTTAACTGTA+AGG	0.486461	1_1:+59310936	None:intergenic
AACAGCATGTGCAGCAGGCC+AGG	0.486850	1_1:-59309641	Msa0032290:three_prime_UTR
ATCTAAGTCCAGTCCACCAT+TGG	0.492671	1_1:+59311012	None:intergenic
GAATACTGCAACTGGTGCTC+AGG	0.493434	1_1:-59311062	Msa0032290:CDS
TGCTACGCCATGACAAGGGC+TGG	0.498360	1_1:+59311421	None:intergenic
AGAGCAGCTTGCTGGCAGAA+TGG	0.507412	1_1:-59309701	Msa0032290:three_prime_UTR
ATTAACTAATAAAGAAGTGC+AGG	0.507677	1_1:-59310157	Msa0032290:three_prime_UTR
CAAGTGCTCCAATGGTGGAC+TGG	0.511136	1_1:-59311020	Msa0032290:CDS
AATGTAGCAATCAGCTCAAA+CGG	0.513795	1_1:-59310817	Msa0032290:three_prime_UTR
CTAATAAAGAAGTGCAGGAT+GGG	0.523391	1_1:-59310152	Msa0032290:three_prime_UTR
CAGGTGACGAATACTGCAAC+TGG	0.524837	1_1:-59311070	Msa0032290:CDS
CCGCAAGAATCTCTGCCTGT+TGG	0.525418	1_1:+59311363	None:intergenic
TGGCCCAGCTGGTCCACAAA+AGG	0.525504	1_1:+59311383	None:intergenic
CAGTGTATACTGTGCTACTT+GGG	0.526139	1_1:-59311451	Msa0032290:CDS
ATGATTTCGCACTTGTACGC+AGG	0.527382	1_1:+59310708	None:intergenic
TTAGGATGTTCTTCCTGAAG+AGG	0.527805	1_1:-59309813	Msa0032290:three_prime_UTR
TCCTTGGTGTAATGTGGAGC+TGG	0.536230	1_1:-59309950	Msa0032290:three_prime_UTR
GTGATTTATTGACTCATCAC+TGG	0.538887	1_1:-59310901	Msa0032290:CDS
TAAGTAAAGTTAGTGTACAT+AGG	0.539496	1_1:+59310342	None:intergenic
TTCAATCAAATTGATACCAA+TGG	0.541175	1_1:-59310980	Msa0032290:CDS
CTTCTGGTAACCCTGTCCAT+TGG	0.545362	1_1:+59310964	None:intergenic
ATGTTGGGACCAATTGTCCT+TGG	0.553024	1_1:-59309966	Msa0032290:three_prime_UTR
TAATCCCAGTTAATATTCTG+AGG	0.556889	1_1:+59311483	None:intergenic
ATCTTCCACTAACATTGTAC+AGG	0.557897	1_1:+59310234	None:intergenic
ACTAATAAAGAAGTGCAGGA+TGG	0.559173	1_1:-59310153	Msa0032290:three_prime_UTR
ATTAACTGAGAAGTGCATGT+TGG	0.560742	1_1:-59309982	Msa0032290:three_prime_UTR
AATGGACAGGGTTACCAGAA+GGG	0.563174	1_1:-59310962	Msa0032290:CDS
AAGACTTGCAGTGCCGTCAA+AGG	0.564665	1_1:-59310057	Msa0032290:three_prime_UTR
TTACATTTAATATAATTTGG+AGG	0.566301	1_1:+59309603	None:intergenic
TTAATCCACTTGCAAAATGA+GGG	0.566597	1_1:-59310435	Msa0032290:three_prime_UTR
TGCAACTGGTGCTCAGGTAA+CGG	0.577038	1_1:-59311056	Msa0032290:CDS
TATAGCTTTGTTAATTGCGA+CGG	0.577389	1_1:-59310923	Msa0032290:CDS
TCAGAGTGCAAACAACGTTA+GGG	0.580216	1_1:-59311526	Msa0032290:CDS
CAACCCTCAGAATATTAACT+GGG	0.582070	1_1:-59311487	Msa0032290:CDS
GTTGAAGGTGTGGCGGAAGG+TGG	0.582680	1_1:-59309579	Msa0032290:three_prime_UTR
ATAGTGGACAAGTGCTCCAA+TGG	0.583102	1_1:-59311028	Msa0032290:CDS
ATGCAAACCAGCCCTTGTCA+TGG	0.583448	1_1:-59311428	Msa0032290:CDS
ATATTTGCTACGCCATGACA+AGG	0.584472	1_1:+59311416	None:intergenic
AGGAAGTTGCCAATACAACC+TGG	0.588413	1_1:+59309623	None:intergenic
CAATTGTCCTTGGTGTAATG+TGG	0.590297	1_1:-59309956	Msa0032290:three_prime_UTR
CAAATTGATACCAATGGACA+GGG	0.591064	1_1:-59310974	Msa0032290:CDS
GCAGCTTGCTGGCAGAATGG+CGG	0.591677	1_1:-59309698	Msa0032290:three_prime_UTR
GTAATAATAGTATACAAACT+TGG	0.596249	1_1:-59309900	Msa0032290:three_prime_UTR
TCAATTCACGTTGACTTACG+TGG	0.599511	1_1:+59310376	None:intergenic
CATGGCGTAGCAAATATGGT+TGG	0.604474	1_1:-59311410	Msa0032290:CDS
TCAGGTAACGGTGAGAATAG+TGG	0.610066	1_1:-59311044	Msa0032290:CDS
TGTGTTTCATAGTAGAAGCA+TGG	0.611414	1_1:+59311633	None:intergenic
CAATGGACAGGGTTACCAGA+AGG	0.617429	1_1:-59310963	Msa0032290:CDS
ATCTAAGACCCTAGGTTAGG+GGG	0.617950	1_1:-59310641	Msa0032290:three_prime_UTR
TTAAAAGAAACCCGAAACAA+AGG	0.621655	1_1:-59310760	Msa0032290:three_prime_UTR
TAGGGTCTTAGATACGCCGC+TGG	0.623707	1_1:+59310651	None:intergenic
GTGGACAAGTGCTCCAATGG+TGG	0.625188	1_1:-59311025	Msa0032290:CDS
AATCCCAGTTAATATTCTGA+GGG	0.629321	1_1:+59311484	None:intergenic
TAAATGTAATGTTGAAGGTG+TGG	0.637124	1_1:-59309589	Msa0032290:three_prime_UTR
TGAATCTATGGAGTTTATGG+AGG	0.638039	1_1:-59309772	Msa0032290:three_prime_UTR
AATGTTGAAGGTGTGGCGGA+AGG	0.641107	1_1:-59309582	Msa0032290:three_prime_UTR
TATCTAAGACCCTAGGTTAG+GGG	0.645324	1_1:-59310642	Msa0032290:three_prime_UTR
ATGTGAACATTCACGTCCAG+CGG	0.654233	1_1:-59310667	Msa0032290:three_prime_UTR
ATGTAATGTTGAAGGTGTGG+CGG	0.655720	1_1:-59309586	Msa0032290:three_prime_UTR
AGGATGGGACCAATTGTCCA+TGG	0.656111	1_1:-59310137	Msa0032290:three_prime_UTR
CCAAGTAGCACAGTATACAC+TGG	0.657659	1_1:+59311452	None:intergenic
TCACTAGCATCGTTCCCACA+AGG	0.659432	1_1:+59311553	None:intergenic
AGCGGCGTATCTAAGACCCT+AGG	0.659664	1_1:-59310649	Msa0032290:three_prime_UTR
TATTTGCTACGCCATGACAA+GGG	0.664421	1_1:+59311417	None:intergenic
CCAACAGGCAGAGATTCTTG+CGG	0.667208	1_1:-59311363	Msa0032290:CDS
TCCAGCTCCACATTACACCA+AGG	0.686873	1_1:+59309949	None:intergenic
TCTGGCTCCATATTACACCA+TGG	0.697603	1_1:+59310120	None:intergenic
GGTTCACTAGATAGTGCATG+CGG	0.724459	1_1:+59310729	None:intergenic
GTGGGAACGATGCTAGTGAG+TGG	0.745826	1_1:-59311549	Msa0032290:CDS

CRISPR-GE

badsite warning	sgRNA_sequence	Strand	Position	Region	GC_content
!!	GAAAAAATTAAAAGTTATAT+AGG	+	chr1_1:59310632-59310651	None:intergenic	10.0%
!!!	GTAAACATAAATATATATTT+TGG	+	chr1_1:59310671-59310690	None:intergenic	10.0%
!!	TATATTTATGTTTACATAAT+TGG	-	chr1_1:59310676-59310695	Msa0032290:three_prime_UTR	10.0%
!!	ACATTACATTTAATATAATT+TGG	+	chr1_1:59311635-59311654	None:intergenic	10.0%
!!	GAAAAAATTAAAAGTTATAT+AGG	+	chr1_1:59310632-59310651	None:intergenic	10.0%
!!!	GTAAACATAAATATATATTT+TGG	+	chr1_1:59310671-59310690	None:intergenic	10.0%
!!	TATATTTATGTTTACATAAT+TGG	-	chr1_1:59310676-59310695	Msa0032290:three_prime_UTR	10.0%
!!	ACATTACATTTAATATAATT+TGG	+	chr1_1:59311635-59311654	None:intergenic	10.0%
!!!	AACATAAATATATATTTTGG+AGG	+	chr1_1:59310668-59310687	None:intergenic	15.0%
!!!	TATTTATTTTGTCTAAAGAA+TGG	-	chr1_1:59311024-59311043	Msa0032290:CDS	15.0%
!!!	AATTTGAAAGATTTTATCTA+CGG	+	chr1_1:59311400-59311419	None:intergenic	15.0%
!!	AGATAAAATCTTTCAAATTT+AGG	-	chr1_1:59311401-59311420	Msa0032290:CDS	15.0%
!!	TTACATTTAATATAATTTGG+AGG	+	chr1_1:59311632-59311651	None:intergenic	15.0%
!!	TATATTAAATGTAATGTTGA+AGG	-	chr1_1:59311638-59311657	Msa0032290:five_prime_UTR	15.0%
!!!	AACATAAATATATATTTTGG+AGG	+	chr1_1:59310668-59310687	None:intergenic	15.0%
!!!	TATTTATTTTGTCTAAAGAA+TGG	-	chr1_1:59311024-59311043	Msa0032290:CDS	15.0%
!!!	AATTTGAAAGATTTTATCTA+CGG	+	chr1_1:59311400-59311419	None:intergenic	15.0%
!!	AGATAAAATCTTTCAAATTT+AGG	-	chr1_1:59311401-59311420	Msa0032290:CDS	15.0%
!!	TTACATTTAATATAATTTGG+AGG	+	chr1_1:59311632-59311651	None:intergenic	15.0%
!!	TATATTAAATGTAATGTTGA+AGG	-	chr1_1:59311638-59311657	Msa0032290:five_prime_UTR	15.0%
!!	TTCAATTAATGATAGTATTG+TGG	-	chr1_1:59310124-59310143	Msa0032290:three_prime_UTR	20.0%
!!!	TGCTACATTAGTTTTATTTA+CGG	+	chr1_1:59310404-59310423	None:intergenic	20.0%
!!!	TTTTTAATATCTCTGGTTAT+TGG	-	chr1_1:59310735-59310754	Msa0032290:three_prime_UTR	20.0%
!!	GTAATAATAGTATACAAACT+TGG	-	chr1_1:59311332-59311351	Msa0032290:intron	20.0%
!!	TTCAATTAATGATAGTATTG+TGG	-	chr1_1:59310124-59310143	Msa0032290:three_prime_UTR	20.0%
!!!	TGCTACATTAGTTTTATTTA+CGG	+	chr1_1:59310404-59310423	None:intergenic	20.0%
!!!	TTTTTAATATCTCTGGTTAT+TGG	-	chr1_1:59310735-59310754	Msa0032290:three_prime_UTR	20.0%
!!	GTAATAATAGTATACAAACT+TGG	-	chr1_1:59311332-59311351	Msa0032290:intron	20.0%
!	AACACACAAAAAAGAGAAAA+TGG	-	chr1_1:59309616-59309635	Msa0032290:three_prime_UTR	25.0%
!!!	TTTTCTAAAATGAGAGAAAG+TGG	+	chr1_1:59309914-59309933	None:intergenic	25.0%
!!	TCACTAGATTTCTTCATTTT+GGG	-	chr1_1:59310011-59310030	Msa0032290:three_prime_UTR	25.0%
!	TTCAATCAAATTGATACCAA+TGG	-	chr1_1:59310252-59310271	Msa0032290:three_prime_UTR	25.0%
!!!	CTACATTAGTTTTATTTACG+GGG	+	chr1_1:59310402-59310421	None:intergenic	25.0%
!!!	GCTACATTAGTTTTATTTAC+GGG	+	chr1_1:59310403-59310422	None:intergenic	25.0%
!!!	TGCTTCATTTTTAATATCTC+TGG	-	chr1_1:59310728-59310747	Msa0032290:three_prime_UTR	25.0%
!	TAAGTAAAGTTAGTGTACAT+AGG	+	chr1_1:59310893-59310912	None:intergenic	25.0%
!	TATGTATAGAATTCACTGTA+TGG	-	chr1_1:59310917-59310936	Msa0032290:CDS	25.0%
!!	ATTAACTAATAAAGAAGTGC+AGG	-	chr1_1:59311075-59311094	Msa0032290:intron	25.0%
!!	TATACAAACTTGGTGAAATT+TGG	-	chr1_1:59311342-59311361	Msa0032290:intron	25.0%
!	AACACACAAAAAAGAGAAAA+TGG	-	chr1_1:59309616-59309635	Msa0032290:three_prime_UTR	25.0%
!!!	TTTTCTAAAATGAGAGAAAG+TGG	+	chr1_1:59309914-59309933	None:intergenic	25.0%
!!	TCACTAGATTTCTTCATTTT+GGG	-	chr1_1:59310011-59310030	Msa0032290:three_prime_UTR	25.0%
!	TTCAATCAAATTGATACCAA+TGG	-	chr1_1:59310252-59310271	Msa0032290:three_prime_UTR	25.0%
!!!	CTACATTAGTTTTATTTACG+GGG	+	chr1_1:59310402-59310421	None:intergenic	25.0%
!!!	GCTACATTAGTTTTATTTAC+GGG	+	chr1_1:59310403-59310422	None:intergenic	25.0%
!!!	TGCTTCATTTTTAATATCTC+TGG	-	chr1_1:59310728-59310747	Msa0032290:three_prime_UTR	25.0%
!	TAAGTAAAGTTAGTGTACAT+AGG	+	chr1_1:59310893-59310912	None:intergenic	25.0%
!	TATGTATAGAATTCACTGTA+TGG	-	chr1_1:59310917-59310936	Msa0032290:CDS	25.0%
!!	ATTAACTAATAAAGAAGTGC+AGG	-	chr1_1:59311075-59311094	Msa0032290:intron	25.0%
!!	TATACAAACTTGGTGAAATT+TGG	-	chr1_1:59311342-59311361	Msa0032290:intron	25.0%
!	GGTGTACAAATTTATTGACT+TGG	+	chr1_1:59309581-59309600	None:intergenic	30.0%
	AATCCCAGTTAATATTCTGA+GGG	+	chr1_1:59309751-59309770	None:intergenic	30.0%
	TAATCCCAGTTAATATTCTG+AGG	+	chr1_1:59309752-59309771	None:intergenic	30.0%
!	CTCACTAGATTTCTTCATTT+TGG	-	chr1_1:59310010-59310029	Msa0032290:three_prime_UTR	30.0%
!	CACTAGATTTCTTCATTTTG+GGG	-	chr1_1:59310012-59310031	Msa0032290:three_prime_UTR	30.0%
!	ATTCTCTTTGACTAGCTAAT+TGG	+	chr1_1:59310085-59310104	None:intergenic	30.0%
	CAAAGCTATAGTTAACTGTA+AGG	+	chr1_1:59310299-59310318	None:intergenic	30.0%
!!	TATAGCTTTGTTAATTGCGA+CGG	-	chr1_1:59310309-59310328	Msa0032290:three_prime_UTR	30.0%
	GAGTACAACTACAACAAAAA+TGG	-	chr1_1:59310448-59310467	Msa0032290:three_prime_UTR	30.0%
	TTAAAAGAAACCCGAAACAA+AGG	-	chr1_1:59310472-59310491	Msa0032290:three_prime_UTR	30.0%
	TTAATCCACTTGCAAAATGA+GGG	-	chr1_1:59310797-59310816	Msa0032290:three_prime_UTR	30.0%
!	ATTTTCCTGTACAATGTTAG+TGG	-	chr1_1:59310993-59311012	Msa0032290:CDS	30.0%
!	TATTTTGTCTAAAGAATGGC+CGG	-	chr1_1:59311028-59311047	Msa0032290:CDS	30.0%
	ACGCATTAGTAAACAATTTC+CGG	+	chr1_1:59311050-59311069	None:intergenic	30.0%
	ACAAAATTTCTCACCTTTGA+CGG	+	chr1_1:59311191-59311210	None:intergenic	30.0%
!	CAAAGGTGAGAAATTTTGTA+TGG	-	chr1_1:59311192-59311211	Msa0032290:intron	30.0%
!	TCAGTTAATTTTACATGCGT+CGG	+	chr1_1:59311239-59311258	None:intergenic	30.0%
!!	GTATGTTTGCTATGAATCTA+TGG	-	chr1_1:59311448-59311467	Msa0032290:CDS	30.0%
	CTATGAATCTATGGAGTTTA+TGG	-	chr1_1:59311457-59311476	Msa0032290:CDS	30.0%
	GAGTAGCAACTCATAAATTT+GGG	-	chr1_1:59311482-59311501	Msa0032290:CDS	30.0%
	TAAATGTAATGTTGAAGGTG+TGG	-	chr1_1:59311643-59311662	Msa0032290:five_prime_UTR	30.0%
!	GGTGTACAAATTTATTGACT+TGG	+	chr1_1:59309581-59309600	None:intergenic	30.0%
	AATCCCAGTTAATATTCTGA+GGG	+	chr1_1:59309751-59309770	None:intergenic	30.0%
	TAATCCCAGTTAATATTCTG+AGG	+	chr1_1:59309752-59309771	None:intergenic	30.0%
!	CTCACTAGATTTCTTCATTT+TGG	-	chr1_1:59310010-59310029	Msa0032290:three_prime_UTR	30.0%
!	CACTAGATTTCTTCATTTTG+GGG	-	chr1_1:59310012-59310031	Msa0032290:three_prime_UTR	30.0%
!	ATTCTCTTTGACTAGCTAAT+TGG	+	chr1_1:59310085-59310104	None:intergenic	30.0%
	CAAAGCTATAGTTAACTGTA+AGG	+	chr1_1:59310299-59310318	None:intergenic	30.0%
!!	TATAGCTTTGTTAATTGCGA+CGG	-	chr1_1:59310309-59310328	Msa0032290:three_prime_UTR	30.0%
	GAGTACAACTACAACAAAAA+TGG	-	chr1_1:59310448-59310467	Msa0032290:three_prime_UTR	30.0%
	TTAAAAGAAACCCGAAACAA+AGG	-	chr1_1:59310472-59310491	Msa0032290:three_prime_UTR	30.0%
	TTAATCCACTTGCAAAATGA+GGG	-	chr1_1:59310797-59310816	Msa0032290:three_prime_UTR	30.0%
!	ATTTTCCTGTACAATGTTAG+TGG	-	chr1_1:59310993-59311012	Msa0032290:CDS	30.0%
!	TATTTTGTCTAAAGAATGGC+CGG	-	chr1_1:59311028-59311047	Msa0032290:CDS	30.0%
	ACGCATTAGTAAACAATTTC+CGG	+	chr1_1:59311050-59311069	None:intergenic	30.0%
	ACAAAATTTCTCACCTTTGA+CGG	+	chr1_1:59311191-59311210	None:intergenic	30.0%
!	CAAAGGTGAGAAATTTTGTA+TGG	-	chr1_1:59311192-59311211	Msa0032290:intron	30.0%
!	TCAGTTAATTTTACATGCGT+CGG	+	chr1_1:59311239-59311258	None:intergenic	30.0%
!!	GTATGTTTGCTATGAATCTA+TGG	-	chr1_1:59311448-59311467	Msa0032290:CDS	30.0%
	CTATGAATCTATGGAGTTTA+TGG	-	chr1_1:59311457-59311476	Msa0032290:CDS	30.0%
	GAGTAGCAACTCATAAATTT+GGG	-	chr1_1:59311482-59311501	Msa0032290:CDS	30.0%
	TAAATGTAATGTTGAAGGTG+TGG	-	chr1_1:59311643-59311662	Msa0032290:five_prime_UTR	30.0%
	TGTGTTTCATAGTAGAAGCA+TGG	+	chr1_1:59309602-59309621	None:intergenic	35.0%
!!!	TTGGTTTTGTGTTTCCTTGT+GGG	-	chr1_1:59309665-59309684	Msa0032290:three_prime_UTR	35.0%
	ACAACCCTCAGAATATTAAC+TGG	-	chr1_1:59309744-59309763	Msa0032290:three_prime_UTR	35.0%
	CAACCCTCAGAATATTAACT+GGG	-	chr1_1:59309745-59309764	Msa0032290:three_prime_UTR	35.0%
!!	TGTGTTGGTCAGATTTTAGA+GGG	+	chr1_1:59309988-59310007	None:intergenic	35.0%
!!	TTGTGTTGGTCAGATTTTAG+AGG	+	chr1_1:59309989-59310008	None:intergenic	35.0%
	GAAATCTAGTGAGTTTGTGT+TGG	+	chr1_1:59310003-59310022	None:intergenic	35.0%
	TAGCTAGTCAAAGAGAATTG+TGG	-	chr1_1:59310087-59310106	Msa0032290:three_prime_UTR	35.0%
	TCAAATTGATACCAATGGAC+AGG	-	chr1_1:59310257-59310276	Msa0032290:three_prime_UTR	35.0%
	CAAATTGATACCAATGGACA+GGG	-	chr1_1:59310258-59310277	Msa0032290:three_prime_UTR	35.0%
!!	GTGATTTATTGACTCATCAC+TGG	-	chr1_1:59310331-59310350	Msa0032290:three_prime_UTR	35.0%
	AATGTAGCAATCAGCTCAAA+CGG	-	chr1_1:59310415-59310434	Msa0032290:three_prime_UTR	35.0%
	CTTAATCCACTTGCAAAATG+AGG	-	chr1_1:59310796-59310815	Msa0032290:three_prime_UTR	35.0%
	GTTAGTGTACATAGGTATAC+TGG	+	chr1_1:59310885-59310904	None:intergenic	35.0%
	ATCTTCCACTAACATTGTAC+AGG	+	chr1_1:59311001-59311020	None:intergenic	35.0%
!	ACTAATAAAGAAGTGCAGGA+TGG	-	chr1_1:59311079-59311098	Msa0032290:intron	35.0%
!	CTAATAAAGAAGTGCAGGAT+GGG	-	chr1_1:59311080-59311099	Msa0032290:intron	35.0%
	CATATTACACCATGGACAAT+TGG	+	chr1_1:59311107-59311126	None:intergenic	35.0%
	CAATTGTCCATGGTGTAATA+TGG	-	chr1_1:59311105-59311124	Msa0032290:intron	35.0%
!	GAGAAATTTTGTATGGAGAG+TGG	-	chr1_1:59311199-59311218	Msa0032290:intron	35.0%
!	ATTAACTGAGAAGTGCATGT+TGG	-	chr1_1:59311250-59311269	Msa0032290:intron	35.0%
!	TTAACTGAGAAGTGCATGTT+GGG	-	chr1_1:59311251-59311270	Msa0032290:intron	35.0%
	CTTGGTGAAATTTGGATTTG+TGG	-	chr1_1:59311350-59311369	Msa0032290:CDS	35.0%
	AACATACACATATCCTCTTC+AGG	+	chr1_1:59311435-59311454	None:intergenic	35.0%
	TGAATCTATGGAGTTTATGG+AGG	-	chr1_1:59311460-59311479	Msa0032290:CDS	35.0%
	GGAGTAGCAACTCATAAATT+TGG	-	chr1_1:59311481-59311500	Msa0032290:CDS	35.0%
	TGTGTTTCATAGTAGAAGCA+TGG	+	chr1_1:59309602-59309621	None:intergenic	35.0%
!!!	TTGGTTTTGTGTTTCCTTGT+GGG	-	chr1_1:59309665-59309684	Msa0032290:three_prime_UTR	35.0%
	ACAACCCTCAGAATATTAAC+TGG	-	chr1_1:59309744-59309763	Msa0032290:three_prime_UTR	35.0%
	CAACCCTCAGAATATTAACT+GGG	-	chr1_1:59309745-59309764	Msa0032290:three_prime_UTR	35.0%
!!	TGTGTTGGTCAGATTTTAGA+GGG	+	chr1_1:59309988-59310007	None:intergenic	35.0%
!!	TTGTGTTGGTCAGATTTTAG+AGG	+	chr1_1:59309989-59310008	None:intergenic	35.0%
	GAAATCTAGTGAGTTTGTGT+TGG	+	chr1_1:59310003-59310022	None:intergenic	35.0%
	TAGCTAGTCAAAGAGAATTG+TGG	-	chr1_1:59310087-59310106	Msa0032290:three_prime_UTR	35.0%
	TCAAATTGATACCAATGGAC+AGG	-	chr1_1:59310257-59310276	Msa0032290:three_prime_UTR	35.0%
	CAAATTGATACCAATGGACA+GGG	-	chr1_1:59310258-59310277	Msa0032290:three_prime_UTR	35.0%
!!	GTGATTTATTGACTCATCAC+TGG	-	chr1_1:59310331-59310350	Msa0032290:three_prime_UTR	35.0%
	AATGTAGCAATCAGCTCAAA+CGG	-	chr1_1:59310415-59310434	Msa0032290:three_prime_UTR	35.0%
	CTTAATCCACTTGCAAAATG+AGG	-	chr1_1:59310796-59310815	Msa0032290:three_prime_UTR	35.0%
	GTTAGTGTACATAGGTATAC+TGG	+	chr1_1:59310885-59310904	None:intergenic	35.0%
	ATCTTCCACTAACATTGTAC+AGG	+	chr1_1:59311001-59311020	None:intergenic	35.0%
!	ACTAATAAAGAAGTGCAGGA+TGG	-	chr1_1:59311079-59311098	Msa0032290:intron	35.0%
!	CTAATAAAGAAGTGCAGGAT+GGG	-	chr1_1:59311080-59311099	Msa0032290:intron	35.0%
	CATATTACACCATGGACAAT+TGG	+	chr1_1:59311107-59311126	None:intergenic	35.0%
	CAATTGTCCATGGTGTAATA+TGG	-	chr1_1:59311105-59311124	Msa0032290:intron	35.0%
!	GAGAAATTTTGTATGGAGAG+TGG	-	chr1_1:59311199-59311218	Msa0032290:intron	35.0%
!	ATTAACTGAGAAGTGCATGT+TGG	-	chr1_1:59311250-59311269	Msa0032290:intron	35.0%
!	TTAACTGAGAAGTGCATGTT+GGG	-	chr1_1:59311251-59311270	Msa0032290:intron	35.0%
	CTTGGTGAAATTTGGATTTG+TGG	-	chr1_1:59311350-59311369	Msa0032290:CDS	35.0%
	AACATACACATATCCTCTTC+AGG	+	chr1_1:59311435-59311454	None:intergenic	35.0%
	TGAATCTATGGAGTTTATGG+AGG	-	chr1_1:59311460-59311479	Msa0032290:CDS	35.0%
	GGAGTAGCAACTCATAAATT+TGG	-	chr1_1:59311481-59311500	Msa0032290:CDS	35.0%
	ACAGAGAACTTTGAGCTTGT+TGG	-	chr1_1:59309646-59309665	Msa0032290:three_prime_UTR	40.0%
!!!	GTTGGTTTTGTGTTTCCTTG+TGG	-	chr1_1:59309664-59309683	Msa0032290:three_prime_UTR	40.0%
	TCAGAGTGCAAACAACGTTA+GGG	-	chr1_1:59309706-59309725	Msa0032290:three_prime_UTR	40.0%
!!	CAGTGTATACTGTGCTACTT+GGG	-	chr1_1:59309781-59309800	Msa0032290:three_prime_UTR	40.0%
!	TATTTGCTACGCCATGACAA+GGG	+	chr1_1:59309818-59309837	None:intergenic	40.0%
!	ATATTTGCTACGCCATGACA+AGG	+	chr1_1:59309819-59309838	None:intergenic	40.0%
	TTGTCATGGCGTAGCAAATA+TGG	-	chr1_1:59309818-59309837	Msa0032290:three_prime_UTR	40.0%
!	GGTATATGTGCCTTTGTTTC+GGG	+	chr1_1:59310485-59310504	None:intergenic	40.0%
!	CGGTATATGTGCCTTTGTTT+CGG	+	chr1_1:59310486-59310505	None:intergenic	40.0%
!	GTATCTAAGACCCTAGGTTA+GGG	-	chr1_1:59310589-59310608	Msa0032290:three_prime_UTR	40.0%
!	TATCTAAGACCCTAGGTTAG+GGG	-	chr1_1:59310590-59310609	Msa0032290:three_prime_UTR	40.0%
!!	TTTTGGAGGACTAAAGTGTG+AGG	+	chr1_1:59310654-59310673	None:intergenic	40.0%
!!	TCAATTCACGTTGACTTACG+TGG	+	chr1_1:59310859-59310878	None:intergenic	40.0%
!	GAATTCACTGTATGGTTTGC+AGG	-	chr1_1:59310925-59310944	Msa0032290:CDS	40.0%
	AAAACATGCTCTGGTGTTTC+TGG	+	chr1_1:59311133-59311152	None:intergenic	40.0%
!	ATGCGTCGGTAAACAATTTC+GGG	+	chr1_1:59311225-59311244	None:intergenic	40.0%
!	CATGCGTCGGTAAACAATTT+CGG	+	chr1_1:59311226-59311245	None:intergenic	40.0%
	CACATTACACCAAGGACAAT+TGG	+	chr1_1:59311278-59311297	None:intergenic	40.0%
!	CAATTGTCCTTGGTGTAATG+TGG	-	chr1_1:59311276-59311295	Msa0032290:intron	40.0%
	TTAGGATGTTCTTCCTGAAG+AGG	-	chr1_1:59311419-59311438	Msa0032290:CDS	40.0%
!	ATGTAATGTTGAAGGTGTGG+CGG	-	chr1_1:59311646-59311665	Msa0032290:five_prime_UTR	40.0%
	ACAGAGAACTTTGAGCTTGT+TGG	-	chr1_1:59309646-59309665	Msa0032290:three_prime_UTR	40.0%
!!!	GTTGGTTTTGTGTTTCCTTG+TGG	-	chr1_1:59309664-59309683	Msa0032290:three_prime_UTR	40.0%
	TCAGAGTGCAAACAACGTTA+GGG	-	chr1_1:59309706-59309725	Msa0032290:three_prime_UTR	40.0%
!!	CAGTGTATACTGTGCTACTT+GGG	-	chr1_1:59309781-59309800	Msa0032290:three_prime_UTR	40.0%
!	TATTTGCTACGCCATGACAA+GGG	+	chr1_1:59309818-59309837	None:intergenic	40.0%
!	ATATTTGCTACGCCATGACA+AGG	+	chr1_1:59309819-59309838	None:intergenic	40.0%
	TTGTCATGGCGTAGCAAATA+TGG	-	chr1_1:59309818-59309837	Msa0032290:three_prime_UTR	40.0%
!	GGTATATGTGCCTTTGTTTC+GGG	+	chr1_1:59310485-59310504	None:intergenic	40.0%
!	CGGTATATGTGCCTTTGTTT+CGG	+	chr1_1:59310486-59310505	None:intergenic	40.0%
!	GTATCTAAGACCCTAGGTTA+GGG	-	chr1_1:59310589-59310608	Msa0032290:three_prime_UTR	40.0%
!	TATCTAAGACCCTAGGTTAG+GGG	-	chr1_1:59310590-59310609	Msa0032290:three_prime_UTR	40.0%
!!	TTTTGGAGGACTAAAGTGTG+AGG	+	chr1_1:59310654-59310673	None:intergenic	40.0%
!!	TCAATTCACGTTGACTTACG+TGG	+	chr1_1:59310859-59310878	None:intergenic	40.0%
!	GAATTCACTGTATGGTTTGC+AGG	-	chr1_1:59310925-59310944	Msa0032290:CDS	40.0%
	AAAACATGCTCTGGTGTTTC+TGG	+	chr1_1:59311133-59311152	None:intergenic	40.0%
!	ATGCGTCGGTAAACAATTTC+GGG	+	chr1_1:59311225-59311244	None:intergenic	40.0%
!	CATGCGTCGGTAAACAATTT+CGG	+	chr1_1:59311226-59311245	None:intergenic	40.0%
	CACATTACACCAAGGACAAT+TGG	+	chr1_1:59311278-59311297	None:intergenic	40.0%
!	CAATTGTCCTTGGTGTAATG+TGG	-	chr1_1:59311276-59311295	Msa0032290:intron	40.0%
	TTAGGATGTTCTTCCTGAAG+AGG	-	chr1_1:59311419-59311438	Msa0032290:CDS	40.0%
!	ATGTAATGTTGAAGGTGTGG+CGG	-	chr1_1:59311646-59311665	Msa0032290:five_prime_UTR	40.0%
	GTCAGAGTGCAAACAACGTT+AGG	-	chr1_1:59309705-59309724	Msa0032290:three_prime_UTR	45.0%
	CCAAGTAGCACAGTATACAC+TGG	+	chr1_1:59309783-59309802	None:intergenic	45.0%
!	CCAGTGTATACTGTGCTACT+TGG	-	chr1_1:59309780-59309799	Msa0032290:three_prime_UTR	45.0%
	CATGGCGTAGCAAATATGGT+TGG	-	chr1_1:59309822-59309841	Msa0032290:three_prime_UTR	45.0%
!	TATGGTTGGACTGCCTTTTG+TGG	-	chr1_1:59309836-59309855	Msa0032290:three_prime_UTR	45.0%
	GTATTCGTCACCTGCATCAT+CGG	+	chr1_1:59310156-59310175	None:intergenic	45.0%
!	TCAGGTAACGGTGAGAATAG+TGG	-	chr1_1:59310188-59310207	Msa0032290:three_prime_UTR	45.0%
!!	ATAGTGGACAAGTGCTCCAA+TGG	-	chr1_1:59310204-59310223	Msa0032290:three_prime_UTR	45.0%
	ATCTAAGTCCAGTCCACCAT+TGG	+	chr1_1:59310223-59310242	None:intergenic	45.0%
	AATGGACAGGGTTACCAGAA+GGG	-	chr1_1:59310270-59310289	Msa0032290:three_prime_UTR	45.0%
	TAACTGTAAGGTGACCCTTC+TGG	+	chr1_1:59310287-59310306	None:intergenic	45.0%
	GGTTCACTAGATAGTGCATG+CGG	+	chr1_1:59310506-59310525	None:intergenic	45.0%
	ATGATTTCGCACTTGTACGC+AGG	+	chr1_1:59310527-59310546	None:intergenic	45.0%
	ATGTGAACATTCACGTCCAG+CGG	-	chr1_1:59310565-59310584	Msa0032290:three_prime_UTR	45.0%
!	CGTATCTAAGACCCTAGGTT+AGG	-	chr1_1:59310588-59310607	Msa0032290:three_prime_UTR	45.0%
!	ATCTAAGACCCTAGGTTAGG+GGG	-	chr1_1:59310591-59310610	Msa0032290:three_prime_UTR	45.0%
!	GTATGCCCTCATTTTGCAAG+TGG	+	chr1_1:59310805-59310824	None:intergenic	45.0%
!	TCTGGCTCCATATTACACCA+TGG	+	chr1_1:59311115-59311134	None:intergenic	45.0%
	CACTGCAGTAAAACATGCTC+TGG	+	chr1_1:59311142-59311161	None:intergenic	45.0%
	ATGTTGGGACCAATTGTCCT+TGG	-	chr1_1:59311266-59311285	Msa0032290:intron	45.0%
	AGGAAGTTGCCAATACAACC+TGG	+	chr1_1:59311612-59311631	None:intergenic	45.0%
	GTCAGAGTGCAAACAACGTT+AGG	-	chr1_1:59309705-59309724	Msa0032290:three_prime_UTR	45.0%
	CCAAGTAGCACAGTATACAC+TGG	+	chr1_1:59309783-59309802	None:intergenic	45.0%
!	CCAGTGTATACTGTGCTACT+TGG	-	chr1_1:59309780-59309799	Msa0032290:three_prime_UTR	45.0%
	CATGGCGTAGCAAATATGGT+TGG	-	chr1_1:59309822-59309841	Msa0032290:three_prime_UTR	45.0%
!	TATGGTTGGACTGCCTTTTG+TGG	-	chr1_1:59309836-59309855	Msa0032290:three_prime_UTR	45.0%
	GTATTCGTCACCTGCATCAT+CGG	+	chr1_1:59310156-59310175	None:intergenic	45.0%
!	TCAGGTAACGGTGAGAATAG+TGG	-	chr1_1:59310188-59310207	Msa0032290:three_prime_UTR	45.0%
!!	ATAGTGGACAAGTGCTCCAA+TGG	-	chr1_1:59310204-59310223	Msa0032290:three_prime_UTR	45.0%
	ATCTAAGTCCAGTCCACCAT+TGG	+	chr1_1:59310223-59310242	None:intergenic	45.0%
	AATGGACAGGGTTACCAGAA+GGG	-	chr1_1:59310270-59310289	Msa0032290:three_prime_UTR	45.0%
	TAACTGTAAGGTGACCCTTC+TGG	+	chr1_1:59310287-59310306	None:intergenic	45.0%
	GGTTCACTAGATAGTGCATG+CGG	+	chr1_1:59310506-59310525	None:intergenic	45.0%
	ATGATTTCGCACTTGTACGC+AGG	+	chr1_1:59310527-59310546	None:intergenic	45.0%
	ATGTGAACATTCACGTCCAG+CGG	-	chr1_1:59310565-59310584	Msa0032290:three_prime_UTR	45.0%
!	CGTATCTAAGACCCTAGGTT+AGG	-	chr1_1:59310588-59310607	Msa0032290:three_prime_UTR	45.0%
!	ATCTAAGACCCTAGGTTAGG+GGG	-	chr1_1:59310591-59310610	Msa0032290:three_prime_UTR	45.0%
!	GTATGCCCTCATTTTGCAAG+TGG	+	chr1_1:59310805-59310824	None:intergenic	45.0%
!	TCTGGCTCCATATTACACCA+TGG	+	chr1_1:59311115-59311134	None:intergenic	45.0%
	CACTGCAGTAAAACATGCTC+TGG	+	chr1_1:59311142-59311161	None:intergenic	45.0%
	ATGTTGGGACCAATTGTCCT+TGG	-	chr1_1:59311266-59311285	Msa0032290:intron	45.0%
	AGGAAGTTGCCAATACAACC+TGG	+	chr1_1:59311612-59311631	None:intergenic	45.0%
	TCACTAGCATCGTTCCCACA+AGG	+	chr1_1:59309682-59309701	None:intergenic	50.0%
	ATGCAAACCAGCCCTTGTCA+TGG	-	chr1_1:59309804-59309823	Msa0032290:three_prime_UTR	50.0%
	CCAACAGGCAGAGATTCTTG+CGG	-	chr1_1:59309869-59309888	Msa0032290:three_prime_UTR	50.0%
	CAGGTGACGAATACTGCAAC+TGG	-	chr1_1:59310162-59310181	Msa0032290:three_prime_UTR	50.0%
	GAATACTGCAACTGGTGCTC+AGG	-	chr1_1:59310170-59310189	Msa0032290:three_prime_UTR	50.0%
	TGCAACTGGTGCTCAGGTAA+CGG	-	chr1_1:59310176-59310195	Msa0032290:three_prime_UTR	50.0%
	CTTCTGGTAACCCTGTCCAT+TGG	+	chr1_1:59310271-59310290	None:intergenic	50.0%
	CAATGGACAGGGTTACCAGA+AGG	-	chr1_1:59310269-59310288	Msa0032290:three_prime_UTR	50.0%
	AGGATGGGACCAATTGTCCA+TGG	-	chr1_1:59311095-59311114	Msa0032290:intron	50.0%
	AAGACTTGCAGTGCCGTCAA+AGG	-	chr1_1:59311175-59311194	Msa0032290:intron	50.0%
!	TCCTTGGTGTAATGTGGAGC+TGG	-	chr1_1:59311282-59311301	Msa0032290:intron	50.0%
	TCCAGCTCCACATTACACCA+AGG	+	chr1_1:59311286-59311305	None:intergenic	50.0%
	ATCAGAACAGCATGTGCAGC+AGG	-	chr1_1:59311586-59311605	Msa0032290:CDS	50.0%
!	AATGTTGAAGGTGTGGCGGA+AGG	-	chr1_1:59311650-59311669	Msa0032290:five_prime_UTR	50.0%
	TCACTAGCATCGTTCCCACA+AGG	+	chr1_1:59309682-59309701	None:intergenic	50.0%
	ATGCAAACCAGCCCTTGTCA+TGG	-	chr1_1:59309804-59309823	Msa0032290:three_prime_UTR	50.0%
	CCAACAGGCAGAGATTCTTG+CGG	-	chr1_1:59309869-59309888	Msa0032290:three_prime_UTR	50.0%
	CAGGTGACGAATACTGCAAC+TGG	-	chr1_1:59310162-59310181	Msa0032290:three_prime_UTR	50.0%
	GAATACTGCAACTGGTGCTC+AGG	-	chr1_1:59310170-59310189	Msa0032290:three_prime_UTR	50.0%
	TGCAACTGGTGCTCAGGTAA+CGG	-	chr1_1:59310176-59310195	Msa0032290:three_prime_UTR	50.0%
	CTTCTGGTAACCCTGTCCAT+TGG	+	chr1_1:59310271-59310290	None:intergenic	50.0%
	CAATGGACAGGGTTACCAGA+AGG	-	chr1_1:59310269-59310288	Msa0032290:three_prime_UTR	50.0%
	AGGATGGGACCAATTGTCCA+TGG	-	chr1_1:59311095-59311114	Msa0032290:intron	50.0%
	AAGACTTGCAGTGCCGTCAA+AGG	-	chr1_1:59311175-59311194	Msa0032290:intron	50.0%
!	TCCTTGGTGTAATGTGGAGC+TGG	-	chr1_1:59311282-59311301	Msa0032290:intron	50.0%
	TCCAGCTCCACATTACACCA+AGG	+	chr1_1:59311286-59311305	None:intergenic	50.0%
	ATCAGAACAGCATGTGCAGC+AGG	-	chr1_1:59311586-59311605	Msa0032290:CDS	50.0%
!	AATGTTGAAGGTGTGGCGGA+AGG	-	chr1_1:59311650-59311669	Msa0032290:five_prime_UTR	50.0%
	GTGGGAACGATGCTAGTGAG+TGG	-	chr1_1:59309683-59309702	Msa0032290:three_prime_UTR	55.0%
!	ACTGCCTTTTGTGGACCAGC+TGG	-	chr1_1:59309845-59309864	Msa0032290:three_prime_UTR	55.0%
!	CTGCCTTTTGTGGACCAGCT+GGG	-	chr1_1:59309846-59309865	Msa0032290:three_prime_UTR	55.0%
	CCGCAAGAATCTCTGCCTGT+TGG	+	chr1_1:59309872-59309891	None:intergenic	55.0%
!	GTGGTGATGACCGATGATGC+AGG	-	chr1_1:59310143-59310162	Msa0032290:three_prime_UTR	55.0%
	GTGGACAAGTGCTCCAATGG+TGG	-	chr1_1:59310207-59310226	Msa0032290:three_prime_UTR	55.0%
!!	CAAGTGCTCCAATGGTGGAC+TGG	-	chr1_1:59310212-59310231	Msa0032290:three_prime_UTR	55.0%
	TAGGGTCTTAGATACGCCGC+TGG	+	chr1_1:59310584-59310603	None:intergenic	55.0%
!	AGCGGCGTATCTAAGACCCT+AGG	-	chr1_1:59310583-59310602	Msa0032290:three_prime_UTR	55.0%
	AGTTCGCACCCCCTAACCTA+GGG	+	chr1_1:59310602-59310621	None:intergenic	55.0%
	AAGTTCGCACCCCCTAACCT+AGG	+	chr1_1:59310603-59310622	None:intergenic	55.0%
	CCAGCAAGCTGCTCTCTCTA+TGG	+	chr1_1:59311526-59311545	None:intergenic	55.0%
	CCATAGAGAGAGCAGCTTGC+TGG	-	chr1_1:59311523-59311542	Msa0032290:CDS	55.0%
!!	AGAGCAGCTTGCTGGCAGAA+TGG	-	chr1_1:59311531-59311550	Msa0032290:CDS	55.0%
	TGCAGCAGGCCAGGTTGTAT+TGG	-	chr1_1:59311600-59311619	Msa0032290:CDS	55.0%
	GTGGGAACGATGCTAGTGAG+TGG	-	chr1_1:59309683-59309702	Msa0032290:three_prime_UTR	55.0%
!	ACTGCCTTTTGTGGACCAGC+TGG	-	chr1_1:59309845-59309864	Msa0032290:three_prime_UTR	55.0%
!	CTGCCTTTTGTGGACCAGCT+GGG	-	chr1_1:59309846-59309865	Msa0032290:three_prime_UTR	55.0%
	CCGCAAGAATCTCTGCCTGT+TGG	+	chr1_1:59309872-59309891	None:intergenic	55.0%
!	GTGGTGATGACCGATGATGC+AGG	-	chr1_1:59310143-59310162	Msa0032290:three_prime_UTR	55.0%
	GTGGACAAGTGCTCCAATGG+TGG	-	chr1_1:59310207-59310226	Msa0032290:three_prime_UTR	55.0%
!!	CAAGTGCTCCAATGGTGGAC+TGG	-	chr1_1:59310212-59310231	Msa0032290:three_prime_UTR	55.0%
	TAGGGTCTTAGATACGCCGC+TGG	+	chr1_1:59310584-59310603	None:intergenic	55.0%
!	AGCGGCGTATCTAAGACCCT+AGG	-	chr1_1:59310583-59310602	Msa0032290:three_prime_UTR	55.0%
	AGTTCGCACCCCCTAACCTA+GGG	+	chr1_1:59310602-59310621	None:intergenic	55.0%
	AAGTTCGCACCCCCTAACCT+AGG	+	chr1_1:59310603-59310622	None:intergenic	55.0%
	CCAGCAAGCTGCTCTCTCTA+TGG	+	chr1_1:59311526-59311545	None:intergenic	55.0%
	CCATAGAGAGAGCAGCTTGC+TGG	-	chr1_1:59311523-59311542	Msa0032290:CDS	55.0%
!!	AGAGCAGCTTGCTGGCAGAA+TGG	-	chr1_1:59311531-59311550	Msa0032290:CDS	55.0%
	TGCAGCAGGCCAGGTTGTAT+TGG	-	chr1_1:59311600-59311619	Msa0032290:CDS	55.0%
	TGCTACGCCATGACAAGGGC+TGG	+	chr1_1:59309814-59309833	None:intergenic	60.0%
	TGGCCCAGCTGGTCCACAAA+AGG	+	chr1_1:59309852-59309871	None:intergenic	60.0%
!!	GCAGCTTGCTGGCAGAATGG+CGG	-	chr1_1:59311534-59311553	Msa0032290:CDS	60.0%
!!	TTGCTGGCAGAATGGCGGAC+TGG	-	chr1_1:59311539-59311558	Msa0032290:CDS	60.0%
!!	TGCTGGCAGAATGGCGGACT+GGG	-	chr1_1:59311540-59311559	Msa0032290:CDS	60.0%
	AACAGCATGTGCAGCAGGCC+AGG	-	chr1_1:59311591-59311610	Msa0032290:CDS	60.0%
!	GTTGAAGGTGTGGCGGAAGG+TGG	-	chr1_1:59311653-59311672	Msa0032290:five_prime_UTR	60.0%
	TGCTACGCCATGACAAGGGC+TGG	+	chr1_1:59309814-59309833	None:intergenic	60.0%
	TGGCCCAGCTGGTCCACAAA+AGG	+	chr1_1:59309852-59309871	None:intergenic	60.0%
!!	GCAGCTTGCTGGCAGAATGG+CGG	-	chr1_1:59311534-59311553	Msa0032290:CDS	60.0%
!!	TTGCTGGCAGAATGGCGGAC+TGG	-	chr1_1:59311539-59311558	Msa0032290:CDS	60.0%
!!	TGCTGGCAGAATGGCGGACT+GGG	-	chr1_1:59311540-59311559	Msa0032290:CDS	60.0%
	AACAGCATGTGCAGCAGGCC+AGG	-	chr1_1:59311591-59311610	Msa0032290:CDS	60.0%
!	GTTGAAGGTGTGGCGGAAGG+TGG	-	chr1_1:59311653-59311672	Msa0032290:five_prime_UTR	60.0%
	TGTGGACCAGCTGGGCCAAC+AGG	-	chr1_1:59309854-59309873	Msa0032290:three_prime_UTR	65.0%
	TCTCTGCCTGTTGGCCCAGC+TGG	+	chr1_1:59309863-59309882	None:intergenic	65.0%
	TGTGGACCAGCTGGGCCAAC+AGG	-	chr1_1:59309854-59309873	Msa0032290:three_prime_UTR	65.0%
	TCTCTGCCTGTTGGCCCAGC+TGG	+	chr1_1:59309863-59309882	None:intergenic	65.0%

Chromosome	Type	Strat	End	Strand	Name
chr1_1	gene	59309572	59311682	59309572	ID=Msa0032290;Name=Msa0032290
chr1_1	mRNA	59309572	59311682	59309572	ID=Msa0032290-mRNA-1;Parent=Msa0032290;Name=Msa0032290-mRNA-1;_AED=0.29;_eAED=0.29;_QI=63\|1\|1\|1\|1\|1\|2\|1293\|164
chr1_1	exon	59309572	59311089	59309572	ID=Msa0032290-mRNA-1:exon:18293;Parent=Msa0032290-mRNA-1
chr1_1	exon	59311350	59311682	59311350	ID=Msa0032290-mRNA-1:exon:18292;Parent=Msa0032290-mRNA-1
chr1_1	five_prime_UTR	59311620	59311682	59311620	ID=Msa0032290-mRNA-1:five_prime_utr;Parent=Msa0032290-mRNA-1
chr1_1	CDS	59311350	59311619	59311350	ID=Msa0032290-mRNA-1:cds;Parent=Msa0032290-mRNA-1
chr1_1	CDS	59310865	59311089	59310865	ID=Msa0032290-mRNA-1:cds;Parent=Msa0032290-mRNA-1
chr1_1	three_prime_UTR	59309572	59310864	59309572	ID=Msa0032290-mRNA-1:three_prime_utr;Parent=Msa0032290-mRNA-1

Gene Sequence

>Msa0032290

ATGGAGAGCACACAGAGAACTTTGAGCTTGTTGGTTTTGTGTTTCCTTGTGGGAACGATGCTAGTGAGTGGTCAGAGTGCAAACAACGTTAGGGCAACATACAACAATTACAACCCTCAGAATATTAACTGGGATTACATTACTGCCAGTGTATACTGTGCTACTTGGGATGCAAACCAGCCCTTGTCATGGCGTAGCAAATATGGTTGGACTGCCTTTTGTGGACCAGCTGGGCCAACAGGCAGAGATTCTTGCGGCAAATGCTTGAGTGTGACGAATACTGCAACTGGTGCTCAGGTAACGGTGAGAATAGTGGACAAGTGCTCCAATGGTGGACTGGACTTAGATGTGAATGTCTTCAATCAAATTGATACCAATGGACAGGGTTACCAGAAGGGTCACCTTACAGTTAACTATAGCTTTGTTAATTGCGACGGTGATTTATTGACTCATCACTGGTGTAATAATTCACTCTTTGTACAAATAAGTAGCTAG

Protein sequence

>Msa0032290

MESTQRTLSLLVLCFLVGTMLVSGQSANNVRATYNNYNPQNINWDYITASVYCATWDANQPLSWRSKYGWTAFCGPAGPTGRDSCGKCLSVTNTATGAQVTVRIVDKCSNGGLDLDVNVFNQIDTNGQGYQKGHLTVNYSFVNCDGDLLTHHWCNNSLFVQISS*