AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa0156490

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0156490 MtrunA17_Chr1g0168031 98.658 447 6 0 1 447 707 1153 0.0 924
Msa0156490 MtrunA17_Chr2g0320751 56.376 447 169 3 1 447 596 1016 0.0 538
Msa0156490 MtrunA17_Chr7g0255821 35.172 435 222 9 3 426 412 797 1.70e-75 252
Msa0156490 MtrunA17_Chr1g0212331 31.907 514 238 11 1 446 569 1038 5.12e-73 248
Msa0156490 MtrunA17_Chr2g0281601 33.540 483 278 11 1 446 825 1301 3.51e-66 230
Msa0156490 MtrunA17_Chr4g0024921 33.111 450 215 14 21 446 546 933 4.66e-66 228
Msa0156490 MtrunA17_Chr5g0393461 35.795 176 106 3 279 447 537 712 8.37e-24 104
Msa0156490 MtrunA17_Chr1g0163011 37.748 151 93 1 279 429 569 718 1.93e-23 103
Msa0156490 MtrunA17_Chr4g0017211 32.461 191 110 4 243 418 1766 1952 8.64e-23 102
Msa0156490 MtrunA17_Chr1g0183681 37.415 147 91 1 301 446 755 901 3.48e-22 100
Msa0156490 MtrunA17_Chr4g0040301 37.594 133 83 0 298 430 397 529 5.78e-22 99.0
Msa0156490 MtrunA17_Chr5g0403921 38.655 119 72 1 297 414 522 640 2.50e-21 97.1
Msa0156490 MtrunA17_Chr3g0137091 32.877 146 94 2 301 442 631 776 3.45e-21 97.1
Msa0156490 MtrunA17_Chr2g0286291 42.063 126 72 1 297 421 508 633 3.81e-21 96.7
Msa0156490 MtrunA17_Chr1g0175191 35.200 125 79 1 299 423 1080 1202 4.26e-21 97.1
Msa0156490 MtrunA17_Chr4g0066741 41.270 126 73 1 297 421 505 630 8.57e-21 95.9
Msa0156490 MtrunA17_Chr8g0384531 33.784 148 97 1 301 447 760 907 9.58e-21 95.5
Msa0156490 MtrunA17_Chr7g0257101 32.716 162 103 2 276 432 1188 1348 1.16e-20 95.5
Msa0156490 MtrunA17_Chr1g0190381 32.796 186 109 4 276 446 184 368 1.82e-19 90.9
Msa0156490 MtrunA17_Chr4g0066651 36.765 136 85 1 296 430 1323 1458 3.59e-19 90.9
Msa0156490 MtrunA17_Chr5g0428401 32.727 165 94 4 297 447 1493 1654 4.44e-19 90.5
Msa0156490 MtrunA17_Chr5g0394201 40.000 125 72 2 301 424 692 814 9.71e-19 89.4
Msa0156490 MtrunA17_Chr1g0205891 40.000 125 72 2 301 424 706 828 1.22e-18 89.0
Msa0156490 MtrunA17_Chr4g0052341 36.585 123 76 1 301 421 1080 1202 1.70e-18 89.0
Msa0156490 MtrunA17_Chr2g0320781 36.538 156 92 3 275 424 88 242 4.32e-18 83.6
Msa0156490 MtrunA17_Chr7g0225791 37.179 156 91 3 275 424 1081 1235 5.05e-18 87.4
Msa0156490 MtrunA17_Chr5g0442141 35.714 126 78 2 301 425 953 1076 9.88e-18 86.3
Msa0156490 MtrunA17_Chr3g0095091 34.921 126 79 2 301 425 907 1030 1.20e-17 86.3
Msa0156490 MtrunA17_Chr7g0233001 36.478 159 94 3 272 424 313 470 1.89e-17 84.7
Msa0156490 MtrunA17_Chr4g0047081 37.179 156 91 3 275 424 1031 1185 2.35e-17 85.1
Msa0156490 MtrunA17_Chr7g0248031 37.500 136 80 3 301 434 1341 1473 5.44e-17 84.3
Msa0156490 MtrunA17_Chr4g0069451 30.114 176 118 3 269 441 456 629 6.81e-17 83.2
Msa0156490 MtrunA17_Chr7g0231521 29.605 152 94 2 295 434 300 450 2.04e-16 82.0
Msa0156490 MtrunA17_Chr8g0348721 42.157 102 58 1 301 401 1333 1434 3.52e-16 81.6
Msa0156490 MtrunA17_Chr7g0232121 28.947 152 95 2 295 434 379 529 9.35e-16 80.1
Msa0156490 MtrunA17_Chr7g0232991 32.168 143 92 2 297 434 477 619 1.92e-14 75.5
Msa0156490 MtrunA17_Chr7g0232971 34.328 134 81 2 298 424 1114 1247 8.80e-14 73.9
Msa0156490 MtrunA17_Chr2g0296421 34.615 130 80 2 298 422 865 994 3.42e-13 72.0
Msa0156490 MtrunA17_Chr7g0233021 34.247 146 87 5 285 424 1039 1181 5.35e-13 71.6
Msa0156490 MtrunA17_Chr7g0233031 36.029 136 76 5 297 424 1062 1194 1.63e-12 70.1
Msa0156490 MtrunA17_Chr8g0352361 33.846 130 81 1 298 422 716 845 1.67e-12 69.7
Msa0156490 MtrunA17_Chr7g0224051 30.612 147 94 4 272 412 911 1055 2.89e-12 68.9
Msa0156490 MtrunA17_Chr3g0083491 31.933 119 75 3 298 414 1170 1284 6.65e-12 68.2
Msa0156490 MtrunA17_Chr3g0086111 29.851 134 90 2 280 409 529 662 6.97e-12 67.8
Msa0156490 MtrunA17_Chr3g0114161 30.556 144 92 4 275 412 1045 1186 3.21e-11 65.9
Msa0156490 MtrunA17_Chr1g0191141 30.769 143 91 4 276 412 901 1041 5.88e-11 65.1
Msa0156490 MtrunA17_Chr5g0437621 30.952 126 81 2 293 412 1117 1242 8.31e-11 64.7
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0156490 AT5G43530.1 65.772 447 124 2 1 446 858 1276 0.0 605
Msa0156490 AT5G22750.1 58.705 448 157 4 1 447 609 1029 0.0 546
Msa0156490 AT5G05130.1 34.061 458 237 10 3 447 475 880 1.39e-72 245
Msa0156490 AT1G61140.3 31.076 502 236 12 1 446 671 1118 4.66e-68 235
Msa0156490 AT1G50410.2 31.836 512 238 12 1 446 513 979 7.90e-68 233
Msa0156490 AT1G50410.1 31.836 512 238 12 1 446 513 979 7.90e-68 233
Msa0156490 AT1G50410.3 31.836 512 238 12 1 446 513 979 7.90e-68 233
Msa0156490 AT1G61140.1 31.076 502 236 12 1 446 829 1276 1.21e-67 234
Msa0156490 AT3G20010.2 31.827 509 237 13 1 446 293 754 6.53e-66 225
Msa0156490 AT3G20010.3 31.827 509 237 13 1 446 318 779 1.01e-65 225
Msa0156490 AT3G20010.1 31.827 509 237 13 1 446 584 1045 2.13e-64 224
Msa0156490 AT3G20010.5 31.827 509 237 13 1 446 584 1045 2.13e-64 224
Msa0156490 AT3G20010.7 31.827 509 237 13 1 446 584 1045 2.13e-64 224
Msa0156490 AT1G05120.1 37.891 256 140 5 210 447 578 832 1.53e-46 172
Msa0156490 AT1G05120.1 27.273 176 89 3 3 143 398 569 8.99e-16 80.5
Msa0156490 AT1G05120.2 37.891 256 140 5 210 447 621 875 1.98e-46 172
Msa0156490 AT1G05120.2 27.273 176 89 3 3 143 441 612 8.07e-16 80.5
Msa0156490 AT3G16600.1 29.792 433 200 13 1 429 255 587 5.75e-44 163
Msa0156490 AT1G11100.5 49.673 153 73 2 298 446 929 1081 4.25e-40 154
Msa0156490 AT1G11100.5 36.719 128 80 1 1 128 613 739 1.49e-22 101
Msa0156490 AT1G11100.1 49.673 153 73 2 298 446 1072 1224 5.48e-40 154
Msa0156490 AT1G11100.1 36.719 128 80 1 1 128 756 882 1.27e-22 101
Msa0156490 AT1G11100.3 49.673 153 73 2 298 446 973 1125 5.48e-40 154
Msa0156490 AT1G11100.3 36.719 128 80 1 1 128 657 783 1.29e-22 101
Msa0156490 AT1G11100.4 49.673 153 73 2 298 446 1116 1268 6.96e-40 153
Msa0156490 AT1G11100.4 36.719 128 80 1 1 128 800 926 1.12e-22 102
Msa0156490 AT1G11100.2 49.673 153 73 2 298 446 1115 1267 7.42e-40 153
Msa0156490 AT1G11100.2 36.719 128 80 1 1 128 799 925 1.18e-22 102
Msa0156490 AT1G02670.7 50.000 152 73 1 299 447 516 667 2.22e-38 147
Msa0156490 AT1G02670.3 50.000 152 73 1 299 447 539 690 2.50e-38 147
Msa0156490 AT1G02670.3 28.488 172 87 3 1 143 300 464 6.13e-15 77.8
Msa0156490 AT1G02670.6 50.000 152 73 1 299 447 512 663 2.86e-38 147
Msa0156490 AT1G02670.6 28.488 172 87 3 1 143 273 437 7.25e-15 77.4
Msa0156490 AT1G02670.1 50.000 152 73 1 299 447 527 678 3.08e-38 147
Msa0156490 AT1G50410.4 27.340 406 188 11 1 344 513 873 3.20e-32 130
Msa0156490 AT1G02670.2 57.426 101 43 0 299 399 539 639 7.64e-30 122
Msa0156490 AT1G02670.2 28.488 172 87 3 1 143 300 464 6.15e-15 77.4
Msa0156490 AT1G02670.5 58.763 97 40 0 299 395 512 608 8.61e-30 122
Msa0156490 AT1G02670.5 28.488 172 87 3 1 143 273 437 7.11e-15 77.4
Msa0156490 AT3G20010.4 27.007 411 180 11 1 345 584 940 9.47e-30 123
Msa0156490 AT3G20010.6 27.007 411 180 11 1 345 584 940 9.47e-30 123
Msa0156490 AT3G20010.8 27.007 411 180 11 1 345 584 940 9.47e-30 123
Msa0156490 AT3G19210.3 39.205 176 100 3 279 447 533 708 3.67e-28 118
Msa0156490 AT3G19210.2 39.205 176 100 3 279 447 531 706 4.39e-28 118
Msa0156490 AT3G19210.1 39.205 176 100 3 279 447 533 708 5.04e-28 118
Msa0156490 AT1G61140.2 34.641 153 98 2 1 153 836 986 9.40e-24 105
Msa0156490 AT5G63950.1 34.483 174 112 2 275 447 727 899 1.91e-23 104
Msa0156490 AT5G63950.2 34.483 174 112 2 275 447 710 882 1.93e-23 104
Msa0156490 AT2G02090.1 37.179 156 96 2 279 434 587 740 7.80e-23 102
Msa0156490 AT1G03750.1 38.095 147 91 0 284 430 531 677 8.01e-23 102
Msa0156490 AT3G54280.1 33.750 160 91 2 274 418 1795 1954 9.42e-23 102
Msa0156490 AT3G54280.2 33.750 160 91 2 274 418 1826 1985 1.00e-22 102
Msa0156490 AT1G05490.1 37.173 191 96 5 277 444 1196 1385 2.95e-22 100
Msa0156490 AT2G18760.3 37.415 147 91 1 301 446 806 952 5.85e-22 99.8
Msa0156490 AT2G18760.4 37.415 147 91 1 301 446 747 893 8.25e-22 99.4
Msa0156490 AT2G18760.1 37.415 147 91 1 301 446 747 893 8.25e-22 99.4
Msa0156490 AT2G18760.2 37.415 147 91 1 301 446 747 893 8.25e-22 99.4
Msa0156490 AT2G18760.7 37.415 147 91 1 301 446 747 893 8.25e-22 99.4
Msa0156490 AT2G18760.5 37.415 147 91 1 301 446 747 893 8.25e-22 99.4
Msa0156490 AT2G18760.6 37.415 147 91 1 301 446 747 893 8.25e-22 99.4
Msa0156490 AT3G57300.2 33.750 160 105 1 276 435 1235 1393 2.03e-21 98.2
Msa0156490 AT3G57300.1 33.750 160 105 1 276 435 1202 1360 2.36e-21 98.2
Msa0156490 AT3G57300.4 33.750 160 105 1 276 435 1202 1360 2.36e-21 98.2
Msa0156490 AT3G12810.2 30.986 142 98 0 295 436 1085 1226 7.37e-21 96.7
Msa0156490 AT3G06400.1 40.476 126 74 1 297 421 506 631 7.38e-21 96.3
Msa0156490 AT3G12810.1 30.986 142 98 0 295 436 1089 1230 7.51e-21 96.7
Msa0156490 AT2G40770.2 29.434 265 153 7 211 445 1344 1604 7.59e-21 96.7
Msa0156490 AT2G40770.1 29.434 265 153 7 211 445 1344 1604 7.59e-21 96.7
Msa0156490 AT2G40770.3 29.434 265 153 7 211 445 1344 1604 7.59e-21 96.7
Msa0156490 AT3G06400.2 40.476 126 74 1 297 421 506 631 7.73e-21 96.3
Msa0156490 AT3G06400.3 40.476 126 74 1 297 421 506 631 8.01e-21 96.3
Msa0156490 AT2G28290.2 36.765 136 85 1 296 430 1088 1223 8.53e-21 96.7
Msa0156490 AT2G28290.6 36.765 136 85 1 296 430 1089 1224 8.53e-21 96.7
Msa0156490 AT2G28290.1 36.765 136 85 1 296 430 1088 1223 8.54e-21 96.7
Msa0156490 AT2G28290.5 36.765 136 85 1 296 430 1089 1224 8.54e-21 96.7
Msa0156490 AT2G28290.3 36.765 136 85 1 296 430 1088 1223 8.76e-21 96.3
Msa0156490 AT2G28290.4 36.765 136 85 1 296 430 1088 1223 8.76e-21 96.3
Msa0156490 AT5G18620.2 40.476 126 74 1 297 421 511 636 1.01e-20 95.9
Msa0156490 AT5G18620.1 40.476 126 74 1 297 421 511 636 1.08e-20 95.9
Msa0156490 AT1G08600.1 34.426 183 104 4 279 446 1093 1274 1.28e-20 95.9
Msa0156490 AT1G08600.4 34.426 183 104 4 279 446 1114 1295 1.36e-20 95.5
Msa0156490 AT1G08600.3 34.426 183 104 4 279 446 1114 1295 1.36e-20 95.5
Msa0156490 AT1G08600.2 34.426 183 104 4 279 446 1114 1295 1.36e-20 95.5
Msa0156490 AT2G25170.3 32.192 146 95 2 301 442 641 786 1.79e-20 95.1
Msa0156490 AT2G25170.2 32.192 146 95 2 301 442 632 777 2.01e-20 95.1
Msa0156490 AT2G25170.1 32.192 146 95 2 301 442 615 760 2.04e-20 95.1
Msa0156490 AT2G25170.4 32.192 146 95 2 301 442 615 760 2.04e-20 95.1
Msa0156490 AT4G31900.2 30.909 165 110 2 261 421 460 624 5.45e-20 93.6
Msa0156490 AT4G31900.1 30.909 165 110 2 261 421 501 665 6.09e-20 93.6
Msa0156490 AT4G31900.3 35.246 122 78 1 301 421 544 665 6.77e-20 93.6
Msa0156490 AT5G66750.1 35.385 130 83 1 296 424 539 668 7.31e-20 93.2
Msa0156490 AT5G44800.1 38.211 123 74 1 301 421 1024 1146 2.96e-19 91.7
Msa0156490 AT5G19310.2 40.800 125 71 2 301 424 451 573 6.99e-19 90.1
Msa0156490 AT5G19310.1 40.800 125 71 2 301 424 715 837 8.05e-19 90.1
Msa0156490 AT2G13370.1 28.358 201 117 4 226 425 908 1082 8.88e-19 90.1
Msa0156490 AT2G13370.2 28.358 201 117 4 226 425 908 1082 8.88e-19 90.1
Msa0156490 AT2G13370.3 28.358 201 117 4 226 425 908 1082 8.88e-19 90.1
Msa0156490 AT3G06010.1 41.600 125 70 2 301 424 733 855 1.05e-18 89.7
Msa0156490 AT2G44980.1 32.609 138 92 1 296 432 383 520 3.18e-17 85.1
Msa0156490 AT3G54460.2 30.667 150 100 2 292 438 891 1039 7.53e-17 84.0
Msa0156490 AT3G54460.1 30.667 150 100 2 292 438 1188 1336 9.05e-17 84.0
Msa0156490 AT2G44980.2 31.081 148 91 1 296 432 392 539 9.65e-17 83.6
Msa0156490 AT2G44980.3 31.081 148 91 1 296 432 391 538 9.91e-17 83.6
Msa0156490 AT3G24340.1 34.320 169 104 3 249 411 896 1063 2.88e-16 82.0
Msa0156490 AT1G48310.2 31.847 157 99 3 272 421 458 613 3.72e-16 81.3
Msa0156490 AT1G48310.1 32.432 148 92 3 272 412 453 599 3.90e-16 81.3
Msa0156490 AT1G48310.3 31.847 157 99 3 272 421 453 608 3.98e-16 81.3
Msa0156490 AT2G46020.2 37.879 132 77 3 301 431 1328 1455 1.45e-15 80.1
Msa0156490 AT2G46020.3 37.879 132 77 3 301 431 1328 1455 1.45e-15 80.1
Msa0156490 AT2G46020.4 37.879 132 77 3 301 431 1328 1455 1.45e-15 80.1
Msa0156490 AT2G46020.5 37.879 132 77 3 301 431 1327 1454 1.48e-15 80.1
Msa0156490 AT2G46020.1 37.879 132 77 3 301 431 1327 1454 1.48e-15 80.1
Msa0156490 AT2G46020.6 37.879 132 77 3 301 431 1327 1454 1.48e-15 80.1
Msa0156490 AT1G02670.4 28.488 172 87 3 1 143 300 464 5.04e-15 77.8
Msa0156490 AT3G42670.2 40.566 106 61 2 309 412 1088 1193 6.45e-14 74.7
Msa0156490 AT3G42670.3 40.566 106 61 2 309 412 1088 1193 6.45e-14 74.7
Msa0156490 AT3G42670.1 40.566 106 61 2 309 412 1088 1193 6.45e-14 74.7
Msa0156490 AT5G07810.1 32.824 131 88 0 296 426 553 683 6.88e-14 74.7
Msa0156490 AT3G42670.4 40.566 106 61 2 309 412 1090 1195 6.92e-14 74.7
Msa0156490 AT2G21450.3 32.090 134 86 1 296 424 544 677 2.25e-13 72.8
Msa0156490 AT2G21450.2 32.090 134 86 1 296 424 642 775 2.41e-13 72.8
Msa0156490 AT2G21450.1 32.090 134 86 1 296 424 635 768 2.51e-13 72.8
Msa0156490 AT5G20420.1 40.566 106 61 2 309 412 1094 1199 4.34e-13 72.0

Find 101 sgRNAs with CRISPR-Local

Find 191 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
AAATTCCCTTGACCAATTTC+AGG 0.167428 1_4:-22944582 None:intergenic
TGGATACATCGATGAGGTTT+TGG 0.202894 1_4:+22945383 Msa0156490:CDS
AGATATCATCTTCTGTTTCT+TGG 0.213521 1_4:-22946734 None:intergenic
TTCTAAGCTCTTCGAATTCT+TGG 0.217802 1_4:+22945650 Msa0156490:CDS
TTCTTTATCCTTTGTTTCCT+TGG 0.222309 1_4:-22944629 None:intergenic
GGTGGTAGTGTTCAACTTAA+TGG 0.255724 1_4:+22946918 Msa0156490:three_prime_UTR
ATGATGAAGTTCGGACTTCC+AGG 0.322572 1_4:+22946770 Msa0156490:CDS
GTGCAACATACTGCTCAAAT+TGG 0.336046 1_4:-22945012 None:intergenic
TTCTCAATATCAACCTTGAA+TGG 0.340545 1_4:-22945603 None:intergenic
TCTTTGATCACCCTGCTCAT+AGG 0.342744 1_4:-22944557 None:intergenic
AATACGATGAATCCTCATTA+TGG 0.355631 1_4:-22946144 None:intergenic
ATAGGATTCTTAAGATTTGA+TGG 0.357413 1_4:+22945768 Msa0156490:CDS
TGGTGTGTGATCCAAACTTT+AGG 0.359808 1_4:+22946974 Msa0156490:three_prime_UTR
TGATCAAAGAGCCCTGAAAT+TGG 0.372770 1_4:+22944570 Msa0156490:CDS
GAGAGTGCCTATTCAATTGC+TGG 0.396973 1_4:+22945499 Msa0156490:CDS
TAATGAGGATTCATCGTATT+GGG 0.405384 1_4:+22946147 Msa0156490:CDS
AAGAAACAGAAGATGATATC+TGG 0.411298 1_4:+22946736 Msa0156490:CDS
CTTTCTTGCGTGTGCAACCT+TGG 0.413656 1_4:+22944385 Msa0156490:CDS
CTTTGATCACCCTGCTCATA+GGG 0.416292 1_4:-22944556 None:intergenic
TCTGCTCAAGTCTGCATACT+TGG 0.418328 1_4:-22945217 None:intergenic
CATCGATGTATCCACGAGAT+TGG 0.440850 1_4:-22945374 None:intergenic
TTGAGTATTTGACGACAGAT+TGG 0.441293 1_4:-22945546 None:intergenic
CAATTGCTGGGGTACCTCAA+TGG 0.444218 1_4:+22945512 Msa0156490:CDS
AATTCCCTTGACCAATTTCA+GGG 0.447512 1_4:-22944581 None:intergenic
TGGAAATTTGGCGGTACTAC+CGG 0.448182 1_4:+22947019 Msa0156490:three_prime_UTR
TCTGTATAGGTGGACTAAGT+TGG 0.452785 1_4:+22944525 Msa0156490:intron
TTAACGAAACAAAAGAGAAA+AGG 0.453574 1_4:+22945832 Msa0156490:CDS
AATTGCTGGGGTACCTCAAT+GGG 0.465911 1_4:+22945513 Msa0156490:CDS
TCTCAATATCAACCTTGAAT+GGG 0.473363 1_4:-22945602 None:intergenic
AGGTACCCCAGCAATTGAAT+AGG 0.477077 1_4:-22945506 None:intergenic
GTGTGCAACCTTGGTGCAGC+TGG 0.480527 1_4:+22944394 Msa0156490:CDS
CACTCACTGATGATGAAGTT+CGG 0.480785 1_4:+22946761 Msa0156490:CDS
AACCTTGGTGCAGCTGGCAG+TGG 0.481522 1_4:+22944400 Msa0156490:CDS
TCACTAAAAGCTGGTGGTGT+TGG 0.484085 1_4:+22945964 Msa0156490:CDS
TCCATCGGAAAGCCCATTCA+AGG 0.485300 1_4:+22945590 Msa0156490:CDS
TAACGAAACAAAAGAGAAAA+GGG 0.493018 1_4:+22945833 Msa0156490:CDS
ATAATGAGGATTCATCGTAT+TGG 0.493417 1_4:+22946146 Msa0156490:CDS
TGGAGAATCCATTGAGGAGG+AGG 0.499507 1_4:+22945742 Msa0156490:CDS
GGTTCAAAAGCCCTATGAGC+AGG 0.499527 1_4:+22944546 Msa0156490:CDS
GAATGAAGCTCACAATGTAT+AGG 0.500039 1_4:+22946897 Msa0156490:three_prime_UTR
ATGGCTTGCTCCTCAACTGC+AGG 0.502455 1_4:-22946125 None:intergenic
ACCTTGAATGGGCTTTCCGA+TGG 0.503767 1_4:-22945591 None:intergenic
TACCATTAGGAAAACATTTG+AGG 0.504959 1_4:-22946004 None:intergenic
CGATCTTATTGTACTTCCAT+CGG 0.510305 1_4:+22945575 Msa0156490:CDS
ATTGAATATCAGTTGGTGGC+AGG 0.512813 1_4:-22944765 None:intergenic
ATCGTATTGGGCAAAAGCGA+AGG 0.513110 1_4:+22946159 Msa0156490:CDS
CAATTTGACGGAGTCTTCAA+AGG 0.513432 1_4:+22945626 Msa0156490:CDS
TGTATCCACGAGATTGGATA+GGG 0.521353 1_4:-22945368 None:intergenic
GACAACTTACTTTAGCTCTC+AGG 0.521549 1_4:-22944840 None:intergenic
TGAACAGTCAGAATCTGAAC+GGG 0.524328 1_4:+22944798 Msa0156490:CDS
AAGGAAACAAAGGATAAAGA+AGG 0.531212 1_4:+22944631 Msa0156490:CDS
AAACAAAGGATAAAGAAGGA+AGG 0.532039 1_4:+22944635 Msa0156490:CDS
TAAAAGCGGGTACTTTCGCT+TGG 0.533208 1_4:+22947048 Msa0156490:three_prime_UTR
GTTGGTGGCAGGACAAGTAT+AGG 0.534691 1_4:-22944754 None:intergenic
TGATATTGAGAACAATTTGA+CGG 0.537534 1_4:+22945614 Msa0156490:CDS
AGAGTGCCTATTCAATTGCT+GGG 0.537910 1_4:+22945500 Msa0156490:CDS
AACAGGGAACAGTGGAAGAT+CGG 0.539243 1_4:+22946695 Msa0156490:intron
GAGAATCCATTGAGGAGGAG+GGG 0.541246 1_4:+22945744 Msa0156490:CDS
ATGTATCCACGAGATTGGAT+AGG 0.549297 1_4:-22945369 None:intergenic
ATCAATTGAATATCAGTTGG+TGG 0.549580 1_4:-22944769 None:intergenic
GCAGTATGTTGCACAAGGAA+AGG 0.550817 1_4:+22945021 Msa0156490:CDS
GGAGAATCCATTGAGGAGGA+GGG 0.553559 1_4:+22945743 Msa0156490:CDS
AGTCAAGGATATTTGCATAG+TGG 0.556170 1_4:-22945051 None:intergenic
AAGCAGAAGCGGAATTCTGT+AGG 0.556774 1_4:-22945341 None:intergenic
TTGTTGATGTCACTAAAAGC+TGG 0.557014 1_4:+22945955 Msa0156490:CDS
TGGACACTTACCACCCATTG+AGG 0.557729 1_4:-22945526 None:intergenic
AGAATCCATTGAGGAGGAGG+GGG 0.559478 1_4:+22945745 Msa0156490:CDS
TGTAAGAAGATTCATTGTGA+AGG 0.568078 1_4:+22946187 Msa0156490:CDS
CTCATTCATTAACGATGGAT+AGG 0.572587 1_4:-22947085 None:intergenic
TCAATCAATTGAATATCAGT+TGG 0.579281 1_4:-22944772 None:intergenic
AAGAGCCCTGAAATTGGTCA+AGG 0.581201 1_4:+22944576 Msa0156490:CDS
TTGAGGAGCAAGCCATAATG+AGG 0.586141 1_4:+22946132 Msa0156490:CDS
ATCTGTTGGAGAATCCATTG+AGG 0.586420 1_4:+22945736 Msa0156490:CDS
TCGTATTGGGCAAAAGCGAA+GGG 0.594361 1_4:+22946160 Msa0156490:CDS
CTACAAAACTGATGTGCACA+TGG 0.602066 1_4:-22945477 None:intergenic
ATGATTCAGTATGAGACTCA+AGG 0.605306 1_4:-22945254 None:intergenic
GATAGGGTTTGAAGCAGAAG+CGG 0.611648 1_4:-22945352 None:intergenic
GAGGAGAACCAAGGAAACAA+AGG 0.619004 1_4:+22944621 Msa0156490:CDS
ACACACTCATTCATTAACGA+TGG 0.619956 1_4:-22947090 None:intergenic
TGTAGAATGTGCTATATGCA+TGG 0.621370 1_4:+22945428 Msa0156490:CDS
CTACCGGTTAAAGTAAAAGC+GGG 0.622355 1_4:+22947035 Msa0156490:three_prime_UTR
TGCTGGGGTACCTCAATGGG+TGG 0.624623 1_4:+22945516 Msa0156490:CDS
TGAAGCTCACAATGTATAGG+TGG 0.629240 1_4:+22946900 Msa0156490:three_prime_UTR
GTGAACAGTCAGAATCTGAA+CGG 0.632197 1_4:+22944797 Msa0156490:CDS
AGAGCCCTGAAATTGGTCAA+GGG 0.632813 1_4:+22944577 Msa0156490:CDS
TCAAACCCTATCCAATCTCG+TGG 0.633197 1_4:+22945363 Msa0156490:CDS
GTTCAAAAGCCCTATGAGCA+GGG 0.633983 1_4:+22944547 Msa0156490:CDS
TCAACTGCATCAGTAAGTCA+AGG 0.634146 1_4:-22945066 None:intergenic
GTGGTGGAATCCTGCAGTTG+AGG 0.634826 1_4:+22946115 Msa0156490:CDS
TTTGAGCAGTATGTTGCACA+AGG 0.648339 1_4:+22945016 Msa0156490:CDS
ACTTACTGATGCAGTTGAGA+CGG 0.651765 1_4:+22945071 Msa0156490:CDS
TGAAAATGTAGGATCCGTGG+TGG 0.652521 1_4:+22946099 Msa0156490:intron
TGTTGGAGAATCCATTGAGG+AGG 0.653975 1_4:+22945739 Msa0156490:CDS
ACTACCGGTTAAAGTAAAAG+CGG 0.681961 1_4:+22947034 Msa0156490:three_prime_UTR
GAGTGCCTATTCAATTGCTG+GGG 0.685646 1_4:+22945501 Msa0156490:CDS
TTGATGTCACTAAAAGCTGG+TGG 0.692474 1_4:+22945958 Msa0156490:CDS
TTATGAAAATGTAGGATCCG+TGG 0.693075 1_4:+22946096 Msa0156490:intron
TACCACTGCCAGCTGCACCA+AGG 0.695747 1_4:-22944402 None:intergenic
ATTGATGTTGAGGAGAACCA+AGG 0.698202 1_4:+22944612 Msa0156490:CDS
TCAACTGCAGGATTCCACCA+CGG 0.703822 1_4:-22946113 None:intergenic
ATCTCGTGGATACATCGATG+AGG 0.750732 1_4:+22945377 Msa0156490:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!!! TTTTTAATTTGCTTCTGTAT+AGG + chr1_4:22944512-22944531 Msa0156490:intron 20.0%
!!! AGTTATTTCACTTTTGTAAT+AGG + chr1_4:22944895-22944914 Msa0156490:intron 20.0%
!! TTATTATTGGTAGAAGAATT+AGG - chr1_4:22945163-22945182 None:intergenic 20.0%
!! ATTGCAAAACTGATTATTAT+TGG - chr1_4:22945176-22945195 None:intergenic 20.0%
!!! TTATTCAATTCTTCATTTTG+CGG - chr1_4:22945306-22945325 None:intergenic 20.0%
!!! AAAGTATTGTTTTTAGTCAA+TGG + chr1_4:22945700-22945719 Msa0156490:CDS 20.0%
!!! AGTTTTTGTTATGAAAATGT+AGG + chr1_4:22946088-22946107 Msa0156490:intron 20.0%
!! AATTATTAGTCAAATGTTCT+TGG + chr1_4:22946314-22946333 Msa0156490:intron 20.0%
!!! TGAACATGTTAATTTTGAAA+AGG + chr1_4:22946339-22946358 Msa0156490:intron 20.0%
!! ATTGAGAAATGCATAAAATA+TGG - chr1_4:22946482-22946501 None:intergenic 20.0%
!! TCATTAATCAACCAAAAATA+GGG - chr1_4:22946655-22946674 None:intergenic 20.0%
!! TTCATTAATCAACCAAAAAT+AGG - chr1_4:22946656-22946675 None:intergenic 20.0%
!!! TTGAGTACTTTTTTATGTTA+TGG + chr1_4:22946954-22946973 Msa0156490:three_prime_UTR 20.0%
!!! TTTGTTTTTTTCCTAAAGTT+TGG - chr1_4:22946988-22947007 None:intergenic 20.0%
! TCAATCAATTGAATATCAGT+TGG - chr1_4:22944775-22944794 None:intergenic 25.0%
! TGATATTGAGAACAATTTGA+CGG + chr1_4:22945614-22945633 Msa0156490:CDS 25.0%
! ATAGGATTCTTAAGATTTGA+TGG + chr1_4:22945768-22945787 Msa0156490:CDS 25.0%
! TTAACGAAACAAAAGAGAAA+AGG + chr1_4:22945832-22945851 Msa0156490:CDS 25.0%
! TAACGAAACAAAAGAGAAAA+GGG + chr1_4:22945833-22945852 Msa0156490:CDS 25.0%
! ATTAATCAACCAAAAATAGG+GGG - chr1_4:22946653-22946672 None:intergenic 25.0%
! CATTAATCAACCAAAAATAG+GGG - chr1_4:22946654-22946673 None:intergenic 25.0%
! TGATTAATGAAAACAATCGT+TGG + chr1_4:22946665-22946684 Msa0156490:intron 25.0%
!! TTAATTTGCTTCTGTATAGG+TGG + chr1_4:22944515-22944534 Msa0156490:intron 30.0%
! TTCTTTATCCTTTGTTTCCT+TGG - chr1_4:22944632-22944651 None:intergenic 30.0%
AAGGAAACAAAGGATAAAGA+AGG + chr1_4:22944631-22944650 Msa0156490:CDS 30.0%
AAACAAAGGATAAAGAAGGA+AGG + chr1_4:22944635-22944654 Msa0156490:CDS 30.0%
ATCAATTGAATATCAGTTGG+TGG - chr1_4:22944772-22944791 None:intergenic 30.0%
CCTGTTTGAATTGAATGTAA+CGG - chr1_4:22944991-22945010 None:intergenic 30.0%
TTGGTAGAAGAATTAGGAAT+GGG - chr1_4:22945157-22945176 None:intergenic 30.0%
ATTGGTAGAAGAATTAGGAA+TGG - chr1_4:22945158-22945177 None:intergenic 30.0%
TCTCAATATCAACCTTGAAT+GGG - chr1_4:22945605-22945624 None:intergenic 30.0%
TTCTCAATATCAACCTTGAA+TGG - chr1_4:22945606-22945625 None:intergenic 30.0%
!!! GACTTCATTTTTTGATCTGT+TGG + chr1_4:22945722-22945741 Msa0156490:CDS 30.0%
CAATGAGGAAAAAGTGAAAA+AGG - chr1_4:22945921-22945940 None:intergenic 30.0%
! TTTCCTCATTGTTCCTTTTT+AGG + chr1_4:22945930-22945949 Msa0156490:intron 30.0%
AAACCTAAAAAGGAACAATG+AGG - chr1_4:22945936-22945955 None:intergenic 30.0%
GACATCAACAAAACCTAAAA+AGG - chr1_4:22945946-22945965 None:intergenic 30.0%
TACCATTAGGAAAACATTTG+AGG - chr1_4:22946007-22946026 None:intergenic 30.0%
AAACTGGTATACATACCATT+AGG - chr1_4:22946020-22946039 None:intergenic 30.0%
AATACGATGAATCCTCATTA+TGG - chr1_4:22946147-22946166 None:intergenic 30.0%
ATAATGAGGATTCATCGTAT+TGG + chr1_4:22946146-22946165 Msa0156490:CDS 30.0%
TAATGAGGATTCATCGTATT+GGG + chr1_4:22946147-22946166 Msa0156490:CDS 30.0%
TGTAAGAAGATTCATTGTGA+AGG + chr1_4:22946187-22946206 Msa0156490:CDS 30.0%
TTAGTGTTATCAACATGTGA+AGG - chr1_4:22946273-22946292 None:intergenic 30.0%
!! GATGTCTTTAGTTAGTTTGT+TGG + chr1_4:22946514-22946533 Msa0156490:intron 30.0%
! AATCTCCTGTATCCTTTTAA+GGG + chr1_4:22946581-22946600 Msa0156490:intron 30.0%
!!! GTATTTGAGGACCTTTTTTT+TGG - chr1_4:22946616-22946635 None:intergenic 30.0%
AGTCAACAAAACAGTATTTG+AGG - chr1_4:22946629-22946648 None:intergenic 30.0%
! AGATATCATCTTCTGTTTCT+TGG - chr1_4:22946737-22946756 None:intergenic 30.0%
AAGAAACAGAAGATGATATC+TGG + chr1_4:22946736-22946755 Msa0156490:CDS 30.0%
! AAAAACAAAGCACACAGTTT+TGG + chr1_4:22946999-22947018 Msa0156490:three_prime_UTR 30.0%
AATTCCCTTGACCAATTTCA+GGG - chr1_4:22944584-22944603 None:intergenic 35.0%
AAATTCCCTTGACCAATTTC+AGG - chr1_4:22944585-22944604 None:intergenic 35.0%
! AATTGGTCAAGGGAATTTTG+AGG + chr1_4:22944587-22944606 Msa0156490:CDS 35.0%
!! TTTTGAGGACATTGATGTTG+AGG + chr1_4:22944602-22944621 Msa0156490:CDS 35.0%
!! CAGTTATGCTATGTTTTTGC+AGG + chr1_4:22944731-22944750 Msa0156490:intron 35.0%
TAGAAACCCAGTAATTAAGG+GGG - chr1_4:22944940-22944959 None:intergenic 35.0%
CTAGAAACCCAGTAATTAAG+GGG - chr1_4:22944941-22944960 None:intergenic 35.0%
CCTAGAAACCCAGTAATTAA+GGG - chr1_4:22944942-22944961 None:intergenic 35.0%
CCTTAATTACTGGGTTTCTA+GGG + chr1_4:22944940-22944959 Msa0156490:intron 35.0%
CCGTTACATTCAATTCAAAC+AGG + chr1_4:22944988-22945007 Msa0156490:intron 35.0%
AGTCAAGGATATTTGCATAG+TGG - chr1_4:22945054-22945073 None:intergenic 35.0%
! TCAGTTTTGCAATTTTGCAG+TGG + chr1_4:22945183-22945202 Msa0156490:intron 35.0%
ATGATTCAGTATGAGACTCA+AGG - chr1_4:22945257-22945276 None:intergenic 35.0%
!!! GTTTTGGGGCATATTCAAAA+GGG + chr1_4:22945399-22945418 Msa0156490:CDS 35.0%
TGTAGAATGTGCTATATGCA+TGG + chr1_4:22945428-22945447 Msa0156490:CDS 35.0%
TTGAGTATTTGACGACAGAT+TGG - chr1_4:22945549-22945568 None:intergenic 35.0%
CGATCTTATTGTACTTCCAT+CGG + chr1_4:22945575-22945594 Msa0156490:CDS 35.0%
TTCTAAGCTCTTCGAATTCT+TGG + chr1_4:22945650-22945669 Msa0156490:CDS 35.0%
TTGTTGATGTCACTAAAAGC+TGG + chr1_4:22945955-22945974 Msa0156490:CDS 35.0%
! TGCCTCAAATGTTTTCCTAA+TGG + chr1_4:22946002-22946021 Msa0156490:CDS 35.0%
TTATGAAAATGTAGGATCCG+TGG + chr1_4:22946096-22946115 Msa0156490:intron 35.0%
TTGCATGAGCTAATAGTTCT+GGG - chr1_4:22946238-22946257 None:intergenic 35.0%
ATTGCATGAGCTAATAGTTC+TGG - chr1_4:22946239-22946258 None:intergenic 35.0%
ATCAACATGTGAAGGTGAAT+AGG - chr1_4:22946265-22946284 None:intergenic 35.0%
TTCTTATCAAACTACATGCG+TGG + chr1_4:22946397-22946416 Msa0156490:intron 35.0%
GCATACAATATCATAGAAGC+CGG - chr1_4:22946423-22946442 None:intergenic 35.0%
CGTGGTCAATGTTTGAAAAA+TGG - chr1_4:22946549-22946568 None:intergenic 35.0%
GAGATTGCTTAAGAAAAACG+TGG - chr1_4:22946567-22946586 None:intergenic 35.0%
! CAATCTCCTGTATCCTTTTA+AGG + chr1_4:22946580-22946599 Msa0156490:intron 35.0%
! ATCTCCTGTATCCTTTTAAG+GGG + chr1_4:22946582-22946601 Msa0156490:intron 35.0%
!!! AATCGTTGGTTTTGTGAACA+GGG + chr1_4:22946679-22946698 Msa0156490:intron 35.0%
!!! CCTATGAGAAAAGCATTTTG+AGG - chr1_4:22946804-22946823 None:intergenic 35.0%
! CCTCAAAATGCTTTTCTCAT+AGG + chr1_4:22946801-22946820 Msa0156490:CDS 35.0%
GAATGAAGCTCACAATGTAT+AGG + chr1_4:22946897-22946916 Msa0156490:three_prime_UTR 35.0%
!!! AGCACACAGTTTTGGAAATT+TGG + chr1_4:22947007-22947026 Msa0156490:three_prime_UTR 35.0%
!!! ACACAGTTTTGGAAATTTGG+CGG + chr1_4:22947010-22947029 Msa0156490:three_prime_UTR 35.0%
! ACTACCGGTTAAAGTAAAAG+CGG + chr1_4:22947034-22947053 Msa0156490:three_prime_UTR 35.0%
CTCATTCATTAACGATGGAT+AGG - chr1_4:22947088-22947107 None:intergenic 35.0%
CGAACAGAACTGTGAAGTAA+AGG - chr1_4:22944486-22944505 None:intergenic 40.0%
!! TCTGTATAGGTGGACTAAGT+TGG + chr1_4:22944525-22944544 Msa0156490:intron 40.0%
TGATCAAAGAGCCCTGAAAT+TGG + chr1_4:22944570-22944589 Msa0156490:CDS 40.0%
ATTGATGTTGAGGAGAACCA+AGG + chr1_4:22944612-22944631 Msa0156490:CDS 40.0%
!! ATTGAATATCAGTTGGTGGC+AGG - chr1_4:22944768-22944787 None:intergenic 40.0%
GTGAACAGTCAGAATCTGAA+CGG + chr1_4:22944797-22944816 Msa0156490:CDS 40.0%
TGAACAGTCAGAATCTGAAC+GGG + chr1_4:22944798-22944817 Msa0156490:CDS 40.0%
!! GACAACTTACTTTAGCTCTC+AGG - chr1_4:22944843-22944862 None:intergenic 40.0%
TTTGTTCCCCCCTTAATTAC+TGG + chr1_4:22944930-22944949 Msa0156490:intron 40.0%
TTGTTCCCCCCTTAATTACT+GGG + chr1_4:22944931-22944950 Msa0156490:intron 40.0%
AGAAACCCAGTAATTAAGGG+GGG - chr1_4:22944939-22944958 None:intergenic 40.0%
CCCTTAATTACTGGGTTTCT+AGG + chr1_4:22944939-22944958 Msa0156490:intron 40.0%
CCCTAGAAACCCAGTAATTA+AGG - chr1_4:22944943-22944962 None:intergenic 40.0%
GTGCAACATACTGCTCAAAT+TGG - chr1_4:22945015-22945034 None:intergenic 40.0%
TTTGAGCAGTATGTTGCACA+AGG + chr1_4:22945016-22945035 Msa0156490:CDS 40.0%
TCAACTGCATCAGTAAGTCA+AGG - chr1_4:22945069-22945088 None:intergenic 40.0%
ACTTACTGATGCAGTTGAGA+CGG + chr1_4:22945071-22945090 Msa0156490:CDS 40.0%
TGTATCCACGAGATTGGATA+GGG - chr1_4:22945371-22945390 None:intergenic 40.0%
ATGTATCCACGAGATTGGAT+AGG - chr1_4:22945372-22945391 None:intergenic 40.0%
! TGGATACATCGATGAGGTTT+TGG + chr1_4:22945383-22945402 Msa0156490:CDS 40.0%
! GGATACATCGATGAGGTTTT+GGG + chr1_4:22945384-22945403 Msa0156490:CDS 40.0%
! GATACATCGATGAGGTTTTG+GGG + chr1_4:22945385-22945404 Msa0156490:CDS 40.0%
!!! GGTTTTGGGGCATATTCAAA+AGG + chr1_4:22945398-22945417 Msa0156490:CDS 40.0%
GGTGTAAAAACAGGATCATC+TGG - chr1_4:22945459-22945478 None:intergenic 40.0%
TGTGCACATGGTGTAAAAAC+AGG - chr1_4:22945468-22945487 None:intergenic 40.0%
CTACAAAACTGATGTGCACA+TGG - chr1_4:22945480-22945499 None:intergenic 40.0%
! AGAGTGCCTATTCAATTGCT+GGG + chr1_4:22945500-22945519 Msa0156490:CDS 40.0%
CAATTTGACGGAGTCTTCAA+AGG + chr1_4:22945626-22945645 Msa0156490:CDS 40.0%
ATCTGTTGGAGAATCCATTG+AGG + chr1_4:22945736-22945755 Msa0156490:CDS 40.0%
TTGATGTCACTAAAAGCTGG+TGG + chr1_4:22945958-22945977 Msa0156490:CDS 40.0%
GCAGTGAGTGTTAGATAAAC+TGG - chr1_4:22946036-22946055 None:intergenic 40.0%
TATCTAACACTCACTGCTTG+TGG + chr1_4:22946039-22946058 Msa0156490:intron 40.0%
! TGCATGAGCTAATAGTTCTG+GGG - chr1_4:22946237-22946256 None:intergenic 40.0%
GGGTCACATGTCCAAAAAAA+AGG + chr1_4:22946602-22946621 Msa0156490:intron 40.0%
!!! TGTTGACTTCCCCCTATTTT+TGG + chr1_4:22946641-22946660 Msa0156490:intron 40.0%
!!! CAATCGTTGGTTTTGTGAAC+AGG + chr1_4:22946678-22946697 Msa0156490:intron 40.0%
! CACTCACTGATGATGAAGTT+CGG + chr1_4:22946761-22946780 Msa0156490:CDS 40.0%
!!! CATTTTGAGGTCTTGAATCC+TGG - chr1_4:22946791-22946810 None:intergenic 40.0%
TGAAGCTCACAATGTATAGG+TGG + chr1_4:22946900-22946919 Msa0156490:three_prime_UTR 40.0%
! GGTGGTAGTGTTCAACTTAA+TGG + chr1_4:22946918-22946937 Msa0156490:three_prime_UTR 40.0%
TGGTGTGTGATCCAAACTTT+AGG + chr1_4:22946974-22946993 Msa0156490:three_prime_UTR 40.0%
! CTACCGGTTAAAGTAAAAGC+GGG + chr1_4:22947035-22947054 Msa0156490:three_prime_UTR 40.0%
!! GTACCCGCTTTTACTTTAAC+CGG - chr1_4:22947041-22947060 None:intergenic 40.0%
GTTCAAAAGCCCTATGAGCA+GGG + chr1_4:22944547-22944566 Msa0156490:CDS 45.0%
CTTTGATCACCCTGCTCATA+GGG - chr1_4:22944559-22944578 None:intergenic 45.0%
TCTTTGATCACCCTGCTCAT+AGG - chr1_4:22944560-22944579 None:intergenic 45.0%
AAGAGCCCTGAAATTGGTCA+AGG + chr1_4:22944576-22944595 Msa0156490:CDS 45.0%
AGAGCCCTGAAATTGGTCAA+GGG + chr1_4:22944577-22944596 Msa0156490:CDS 45.0%
GAGGAGAACCAAGGAAACAA+AGG + chr1_4:22944621-22944640 Msa0156490:CDS 45.0%
GCAGTATGTTGCACAAGGAA+AGG + chr1_4:22945021-22945040 Msa0156490:CDS 45.0%
!!! ACCATCCGTTTTTGGTCATG+AGG + chr1_4:22945101-22945120 Msa0156490:CDS 45.0%
ACCTCATGACCAAAAACGGA+TGG - chr1_4:22945105-22945124 None:intergenic 45.0%
!!! TCCGTTTTTGGTCATGAGGT+CGG + chr1_4:22945105-22945124 Msa0156490:intron 45.0%
ACCGACCTCATGACCAAAAA+CGG - chr1_4:22945109-22945128 None:intergenic 45.0%
TCTGCTCAAGTCTGCATACT+TGG - chr1_4:22945220-22945239 None:intergenic 45.0%
AAGCAGAAGCGGAATTCTGT+AGG - chr1_4:22945344-22945363 None:intergenic 45.0%
! GATAGGGTTTGAAGCAGAAG+CGG - chr1_4:22945355-22945374 None:intergenic 45.0%
TCAAACCCTATCCAATCTCG+TGG + chr1_4:22945363-22945382 Msa0156490:CDS 45.0%
CATCGATGTATCCACGAGAT+TGG - chr1_4:22945377-22945396 None:intergenic 45.0%
ATCTCGTGGATACATCGATG+AGG + chr1_4:22945377-22945396 Msa0156490:CDS 45.0%
!! GAGAGTGCCTATTCAATTGC+TGG + chr1_4:22945499-22945518 Msa0156490:CDS 45.0%
GAGTGCCTATTCAATTGCTG+GGG + chr1_4:22945501-22945520 Msa0156490:CDS 45.0%
AGGTACCCCAGCAATTGAAT+AGG - chr1_4:22945509-22945528 None:intergenic 45.0%
! AATTGCTGGGGTACCTCAAT+GGG + chr1_4:22945513-22945532 Msa0156490:CDS 45.0%
TGTTGGAGAATCCATTGAGG+AGG + chr1_4:22945739-22945758 Msa0156490:CDS 45.0%
GTGAAAAAGGTCAGCTACTC+AGG - chr1_4:22945908-22945927 None:intergenic 45.0%
! TCACTAAAAGCTGGTGGTGT+TGG + chr1_4:22945964-22945983 Msa0156490:CDS 45.0%
TGAAAATGTAGGATCCGTGG+TGG + chr1_4:22946099-22946118 Msa0156490:intron 45.0%
TTGAGGAGCAAGCCATAATG+AGG + chr1_4:22946132-22946151 Msa0156490:CDS 45.0%
ATCGTATTGGGCAAAAGCGA+AGG + chr1_4:22946159-22946178 Msa0156490:CDS 45.0%
TCGTATTGGGCAAAAGCGAA+GGG + chr1_4:22946160-22946179 Msa0156490:CDS 45.0%
TATCAAACTACATGCGTGGC+CGG + chr1_4:22946401-22946420 Msa0156490:intron 45.0%
GTGACCCCTTAAAAGGATAC+AGG - chr1_4:22946589-22946608 None:intergenic 45.0%
TGGACATGTGACCCCTTAAA+AGG - chr1_4:22946596-22946615 None:intergenic 45.0%
! GTTTTGTGAACAGGGAACAG+TGG + chr1_4:22946687-22946706 Msa0156490:intron 45.0%
AACAGGGAACAGTGGAAGAT+CGG + chr1_4:22946695-22946714 Msa0156490:intron 45.0%
ATGATGAAGTTCGGACTTCC+AGG + chr1_4:22946770-22946789 Msa0156490:CDS 45.0%
TGGAAATTTGGCGGTACTAC+CGG + chr1_4:22947019-22947038 Msa0156490:three_prime_UTR 45.0%
TAAAAGCGGGTACTTTCGCT+TGG + chr1_4:22947048-22947067 Msa0156490:three_prime_UTR 45.0%
CTTTCTTGCGTGTGCAACCT+TGG + chr1_4:22944385-22944404 Msa0156490:CDS 50.0%
GGTTCAAAAGCCCTATGAGC+AGG + chr1_4:22944546-22944565 Msa0156490:CDS 50.0%
!! GTTGGTGGCAGGACAAGTAT+AGG - chr1_4:22944757-22944776 None:intergenic 50.0%
! GTGCTGCAACCATCCGTTTT+TGG + chr1_4:22945093-22945112 Msa0156490:CDS 50.0%
! TTTTGGTCATGAGGTCGGTG+TGG + chr1_4:22945110-22945129 Msa0156490:intron 50.0%
! CAATTGCTGGGGTACCTCAA+TGG + chr1_4:22945512-22945531 Msa0156490:CDS 50.0%
TGGACACTTACCACCCATTG+AGG - chr1_4:22945529-22945548 None:intergenic 50.0%
TCCATCGGAAAGCCCATTCA+AGG + chr1_4:22945590-22945609 Msa0156490:CDS 50.0%
! ACCTTGAATGGGCTTTCCGA+TGG - chr1_4:22945594-22945613 None:intergenic 50.0%
TGGAGAATCCATTGAGGAGG+AGG + chr1_4:22945742-22945761 Msa0156490:CDS 50.0%
GGAGAATCCATTGAGGAGGA+GGG + chr1_4:22945743-22945762 Msa0156490:CDS 50.0%
GAGAATCCATTGAGGAGGAG+GGG + chr1_4:22945744-22945763 Msa0156490:CDS 50.0%
AGAATCCATTGAGGAGGAGG+GGG + chr1_4:22945745-22945764 Msa0156490:CDS 50.0%
CACTGCTTGTGGTTGTGATG+TGG + chr1_4:22946050-22946069 Msa0156490:intron 50.0%
TCAACTGCAGGATTCCACCA+CGG - chr1_4:22946116-22946135 None:intergenic 50.0%
! GTGGTGGAATCCTGCAGTTG+AGG + chr1_4:22946115-22946134 Msa0156490:CDS 55.0%
!! ATGGCTTGCTCCTCAACTGC+AGG - chr1_4:22946128-22946147 None:intergenic 55.0%
! GTGTGCAACCTTGGTGCAGC+TGG + chr1_4:22944394-22944413 Msa0156490:CDS 60.0%
! AACCTTGGTGCAGCTGGCAG+TGG + chr1_4:22944400-22944419 Msa0156490:CDS 60.0%
TACCACTGCCAGCTGCACCA+AGG - chr1_4:22944405-22944424 None:intergenic 60.0%
! TGCTGGGGTACCTCAATGGG+TGG + chr1_4:22945516-22945535 Msa0156490:CDS 60.0%
CCTATCCCCCTCCTCCTCAA+TGG - chr1_4:22945753-22945772 None:intergenic 60.0%
CCATTGAGGAGGAGGGGGAT+AGG + chr1_4:22945750-22945769 Msa0156490:CDS 60.0%
Chromosome Type Strat End Strand Name
chr1_4 gene 22944360 22947107 22944360 ID=Msa0156490;Name=Msa0156490
chr1_4 mRNA 22944360 22947107 22944360 ID=Msa0156490-mRNA-1;Parent=Msa0156490;Name=Msa0156490-mRNA-1;_AED=0.01;_eAED=0.01;_QI=0|1|0.87|1|1|1|8|285|447
chr1_4 exon 22944360 22944421 22944360 ID=Msa0156490-mRNA-1:exon:8082;Parent=Msa0156490-mRNA-1
chr1_4 exon 22944534 22944656 22944534 ID=Msa0156490-mRNA-1:exon:8083;Parent=Msa0156490-mRNA-1
chr1_4 exon 22944753 22944852 22944753 ID=Msa0156490-mRNA-1:exon:8084;Parent=Msa0156490-mRNA-1
chr1_4 exon 22945010 22945122 22945010 ID=Msa0156490-mRNA-1:exon:8085;Parent=Msa0156490-mRNA-1
chr1_4 exon 22945203 22945854 22945203 ID=Msa0156490-mRNA-1:exon:8086;Parent=Msa0156490-mRNA-1
chr1_4 exon 22945952 22946023 22945952 ID=Msa0156490-mRNA-1:exon:8087;Parent=Msa0156490-mRNA-1
chr1_4 exon 22946110 22946208 22946110 ID=Msa0156490-mRNA-1:exon:8088;Parent=Msa0156490-mRNA-1
chr1_4 exon 22946700 22947107 22946700 ID=Msa0156490-mRNA-1:exon:8089;Parent=Msa0156490-mRNA-1
chr1_4 CDS 22944360 22944421 22944360 ID=Msa0156490-mRNA-1:cds;Parent=Msa0156490-mRNA-1
chr1_4 CDS 22944534 22944656 22944534 ID=Msa0156490-mRNA-1:cds;Parent=Msa0156490-mRNA-1
chr1_4 CDS 22944753 22944852 22944753 ID=Msa0156490-mRNA-1:cds;Parent=Msa0156490-mRNA-1
chr1_4 CDS 22945010 22945122 22945010 ID=Msa0156490-mRNA-1:cds;Parent=Msa0156490-mRNA-1
chr1_4 CDS 22945203 22945854 22945203 ID=Msa0156490-mRNA-1:cds;Parent=Msa0156490-mRNA-1
chr1_4 CDS 22945952 22946023 22945952 ID=Msa0156490-mRNA-1:cds;Parent=Msa0156490-mRNA-1
chr1_4 CDS 22946110 22946208 22946110 ID=Msa0156490-mRNA-1:cds;Parent=Msa0156490-mRNA-1
chr1_4 CDS 22946700 22946822 22946700 ID=Msa0156490-mRNA-1:cds;Parent=Msa0156490-mRNA-1
chr1_4 three_prime_UTR 22946823 22947107 22946823 ID=Msa0156490-mRNA-1:three_prime_utr;Parent=Msa0156490-mRNA-1
Gene Sequence

>Msa0156490

TTGGAAGATCTATTCAGTCTCTTGTCTTTCTTGCGTGTGCAACCTTGGTGCAGCTGGCAGTGGTGGACTAAGTTGGTTCAAAAGCCCTATGAGCAGGGTGATCAAAGAGCCCTGAAATTGGTCAAGGGAATTTTGAGGACATTGATGTTGAGGAGAACCAAGGAAACAAAGGATAAAGAAGGAAGGCCTATACTTGTCCTGCCACCAACTGATATTCAATTGATTGAGTGTGAACAGTCAGAATCTGAACGGGACTTCTATGATGCTCTCTTCCTGAGAGCTAAAGTCCAATTTGAGCAGTATGTTGCACAAGGAAAGGTTCTTAACCACTATGCAAATATCCTTGACTTACTGATGCAGTTGAGACGGTGCTGCAACCATCCGTTTTTGGTCATGAGTGGAAGTGATACTGCCAAGTATGCAGACTTGAGCAGACTTGCAAGAAAATTCCTTGAGTCTCATACTGAATCATCTGATATGTGCTGTGAAAGTGATGCACCGCAAAATGAAGAATTGAATAAACTTGCTAGTAATTTCCTACAGAATTCCGCTTCTGCTTCAAACCCTATCCAATCTCGTGGATACATCGATGAGGTTTTGGGGCATATTCAAAAGGGTGAAACTGTAGAATGTGCTATATGCATGGAGTCACCAGATGATCCTGTTTTTACACCATGTGCACATCAGTTTTGTAGAGAGTGCCTATTCAATTGCTGGGGTACCTCAATGGGTGGTAAGTGTCCAATCTGTCGTCAAATACTCAAGAAAAACGATCTTATTGTACTTCCATCGGAAAGCCCATTCAAGGTTGATATTGAGAACAATTTGACGGAGTCTTCAAAGGTTTCTAAGCTCTTCGAATTCTTGGAACATAGTCAGATATATTCTGATGAAAAAAGTATTGTTTTTAGTCAATGGACTTCATTTTTTGATCTGTTGGAGAATCCATTGAGGAGGAGGGGGATAGGATTCTTAAGATTTGATGGAAAGTTGACTCAGAAGCAGAGAGAAAAAGTTCTGAAAGAATTTAACGAAACAAAAGAGAAAAGGGTTTTGTTGATGTCACTAAAAGCTGGTGGTGTTGGCTTGAACTTGACTGCTGCCTCAAATGTTTTCCTAATGGATCCGTGGTGGAATCCTGCAGTTGAGGAGCAAGCCATAATGAGGATTCATCGTATTGGGCAAAAGCGAAGGGTTACTGTAAGAAGATTCATTGTGAAGGGAACAGTGGAAGATCGGTTGCAACAAGTGCAAGCCAAGAAACAGAAGATGATATCTGGTGCACTCACTGATGATGAAGTTCGGACTTCCAGGATTCAAGACCTCAAAATGCTTTTCTCATAG
Protein sequence

>Msa0156490

LEDLFSLLSFLRVQPWCSWQWWTKLVQKPYEQGDQRALKLVKGILRTLMLRRTKETKDKEGRPILVLPPTDIQLIECEQSESERDFYDALFLRAKVQFEQYVAQGKVLNHYANILDLLMQLRRCCNHPFLVMSGSDTAKYADLSRLARKFLESHTESSDMCCESDAPQNEELNKLASNFLQNSASASNPIQSRGYIDEVLGHIQKGETVECAICMESPDDPVFTPCAHQFCRECLFNCWGTSMGGKCPICRQILKKNDLIVLPSESPFKVDIENNLTESSKVSKLFEFLEHSQIYSDEKSIVFSQWTSFFDLLENPLRRRGIGFLRFDGKLTQKQREKVLKEFNETKEKRVLLMSLKAGGVGLNLTAASNVFLMDPWWNPAVEEQAIMRIHRIGQKRRVTVRRFIVKGTVEDRLQQVQAKKQKMISGALTDDEVRTSRIQDLKMLFS*