AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa0580730

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0580730 MtrunA17_Chr4g0067951 97.468 158 4 0 1 158 1 158 2.68e-108 305
Msa0580730 MtrunA17_Chr6g0462311 96.203 158 6 0 1 158 1 158 4.08e-107 302
Msa0580730 MtrunA17_Chr6g0462291 84.049 163 21 1 1 158 1 163 1.48e-92 266
Msa0580730 MtrunA17_Chr2g0295064 74.051 158 41 0 1 158 68 225 2.60e-81 239
Msa0580730 MtrunA17_Chr2g0295021 72.611 157 43 0 1 157 1 157 5.38e-80 233
Msa0580730 MtrunA17_Chr2g0295001 72.611 157 43 0 1 157 1 157 5.38e-80 233
Msa0580730 MtrunA17_Chr2g0295051 70.064 157 47 0 1 157 1 157 7.62e-78 228
Msa0580730 MtrunA17_Chr2g0295041 68.354 158 50 0 1 158 1 158 1.47e-75 222
Msa0580730 MtrunA17_Chr2g0295141 63.694 157 57 0 1 157 1 157 1.35e-68 204
Msa0580730 MtrunA17_Chr2g0295104 64.331 157 56 0 1 157 1 157 2.52e-68 204
Msa0580730 MtrunA17_Chr2g0295091 64.331 157 56 0 1 157 1 157 8.50e-68 202
Msa0580730 MtrunA17_Chr2g0295124 64.968 157 55 0 1 157 1 157 8.66e-67 200
Msa0580730 MtrunA17_Chr2g0295081 54.140 157 72 0 1 157 1 157 1.81e-54 169
Msa0580730 MtrunA17_Chr4g0067921 45.963 161 82 4 1 158 1 159 2.51e-37 125
Msa0580730 MtrunA17_Chr2g0295211 37.179 156 97 1 1 155 1 156 2.68e-33 115
Msa0580730 MtrunA17_Chr4g0067841 36.943 157 98 1 1 156 1 157 1.02e-32 114
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score

Find 68 sgRNAs with CRISPR-Local

Find 170 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
ACAACAACATAAATATTATT+TGG 0.112159 4_1:+85937061 None:intergenic
CCATGTTTGGTTTGATAATT+TGG 0.145886 4_1:+85937933 None:intergenic
AAAACAACATAAATCTTATT+TGG 0.160723 4_1:+85937091 None:intergenic
TTTGGTGATGAAGTTGTAAA+AGG 0.190525 4_1:+85937951 None:intergenic
CTCCATCAATTGCATCAATT+TGG 0.202998 4_1:+85937564 None:intergenic
TTGATGGCATCAACAGCTTT+TGG 0.227157 4_1:+85937821 None:intergenic
GATATCATTTGAAGCGAAAT+TGG 0.228714 4_1:-85937480 Msa0580730:CDS
TAGATGGATTAGTTGTAATT+AGG 0.259719 4_1:+85937308 None:intergenic
GGCCATTGAGGGTTATGTTT+TGG 0.293726 4_1:-85937336 Msa0580730:CDS
AACGGTTTCAACACTCTTGA+TGG 0.328088 4_1:+85937805 None:intergenic
GGTCCAAATGGAGGGTCTAT+TGG 0.333504 4_1:-85937452 Msa0580730:CDS
TCCTTTGGTTTGATATTTAA+CGG 0.347214 4_1:+85937421 None:intergenic
TGTTGTTAAGTTTATTTACT+TGG 0.354046 4_1:-85937007 Msa0580730:three_prime_UTR
CACCAAATTGATGCAATTGA+TGG 0.359913 4_1:-85937566 Msa0580730:CDS
TAGTAGGTAGACTTGGTAGA+TGG 0.361526 4_1:+85937292 None:intergenic
CTTTGAACAACAACCGGTAA+TGG 0.364496 4_1:+85936953 None:intergenic
GTCCAAATGGAGGGTCTATT+GGG 0.390136 4_1:-85937451 Msa0580730:CDS
AATTGGCTTAGCATCTCCTT+TGG 0.398977 4_1:+85937406 None:intergenic
TATAGTATAGTTGGAGGAGT+AGG 0.400784 4_1:-85937524 Msa0580730:CDS
TGCAATTGATGGAGCAAACT+TGG 0.407405 4_1:-85937555 Msa0580730:CDS
AATCAATAAATTAATACAAA+TGG 0.412812 4_1:-85937123 Msa0580730:three_prime_UTR
TTGAGAGCAAAATCCATTAC+CGG 0.415935 4_1:-85936966 Msa0580730:three_prime_UTR
TTCCCAATAGACCCTCCATT+TGG 0.416216 4_1:+85937449 None:intergenic
AATGTGAATACACCCATGTT+TGG 0.426195 4_1:+85937920 None:intergenic
AGAGCTTTGTAAAGCTTAGC+AGG 0.435032 4_1:+85937869 None:intergenic
AACGAAAGTGAGCTTCTTGA+CGG 0.435771 4_1:+85937760 None:intergenic
ACAGATGATAGTAGATTAGT+AGG 0.436784 4_1:+85937276 None:intergenic
TTGGTGATGAAGTTGTAAAA+GGG 0.438115 4_1:+85937952 None:intergenic
AAGAAGCTCACTTTCGTTGA+GGG 0.454166 4_1:-85937755 Msa0580730:intron
GGATTAGTTGTAATTAGGAT+TGG 0.464473 4_1:+85937313 None:intergenic
GTGAGCTTCTTGACGGTGCC+AGG 0.467117 4_1:+85937767 None:intergenic
CCAAATTATCAAACCAAACA+TGG 0.473191 4_1:-85937933 Msa0580730:exon
GGTTGAGGAAGGCAAGGCAA+AGG 0.481278 4_1:-85937375 Msa0580730:CDS
GTTGAAACCGTTGAGGGAAA+TGG 0.483004 4_1:-85937794 Msa0580730:CDS
TATGTATGTTGGTGTGTGGC+TGG 0.486530 4_1:+85938013 None:intergenic
TTTGAAGCGAAATTGGTTGA+AGG 0.487809 4_1:-85937473 Msa0580730:CDS
GGGTATAACTATAGTATAGT+TGG 0.489142 4_1:-85937533 Msa0580730:CDS
ATAGTATAGTTGGAGGAGTA+GGG 0.491450 4_1:-85937523 Msa0580730:CDS
AGTAGATTAGTAGGTAGACT+TGG 0.500749 4_1:+85937285 None:intergenic
AGATGCTAAGCCAATTGAAA+AGG 0.515464 4_1:-85937399 Msa0580730:CDS
GTTGAGGGAAATGGTGGTCC+TGG 0.519714 4_1:-85937785 Msa0580730:CDS
GCCAATTGAAAAGGAGGTTG+AGG 0.520188 4_1:-85937390 Msa0580730:CDS
GGCCAAAACATAACCCTCAA+TGG 0.521409 4_1:+85937334 None:intergenic
CAAGAAGCTCACTTTCGTTG+AGG 0.534631 4_1:-85937756 Msa0580730:CDS
GCAATTGATGGAGCAAACTT+GGG 0.541306 4_1:-85937554 Msa0580730:CDS
GTTGAGGAAGGCAAGGCAAA+GGG 0.544771 4_1:-85937374 Msa0580730:CDS
AAGAGTGTTGAAACCGTTGA+GGG 0.546792 4_1:-85937800 Msa0580730:CDS
AATTTGGTGCAACACGTACA+AGG 0.554848 4_1:+85937580 None:intergenic
TGAGAACAAGATATGTATGT+TGG 0.557908 4_1:+85938002 None:intergenic
CAACAAAACACCAAATCTCC+TGG 0.558696 4_1:+85937160 None:intergenic
GGATGATATCATCAGAGTCA+TGG 0.567722 4_1:+85937843 None:intergenic
AAATTGGTTGAAGGTCCAAA+TGG 0.568255 4_1:-85937464 Msa0580730:CDS
AGGACCACCATTTCCCTCAA+CGG 0.572788 4_1:+85937787 None:intergenic
TATAACTATAGTATAGTTGG+AGG 0.584231 4_1:-85937530 Msa0580730:CDS
AGCAGGAGCAACTGTAGAGG+TGG 0.585421 4_1:+85937886 None:intergenic
CTTAGCAGGAGCAACTGTAG+AGG 0.591469 4_1:+85937883 None:intergenic
TGCTAAGCCAATTGAAAAGG+AGG 0.591628 4_1:-85937396 Msa0580730:CDS
ACCGTTAAATATCAAACCAA+AGG 0.596206 4_1:-85937422 Msa0580730:CDS
AGGAGTAGGGTTGCCAGACA+CGG 0.598102 4_1:-85937510 Msa0580730:CDS
TGGTTGAAGGTCCAAATGGA+GGG 0.601542 4_1:-85937460 Msa0580730:CDS
TTGGTTGAAGGTCCAAATGG+AGG 0.609517 4_1:-85937461 Msa0580730:CDS
ATTGAAAAGGAGGTTGAGGA+AGG 0.616285 4_1:-85937386 Msa0580730:CDS
CAAGAGTGTTGAAACCGTTG+AGG 0.622991 4_1:-85937801 Msa0580730:CDS
AAAGGAGGTTGAGGAAGGCA+AGG 0.629508 4_1:-85937381 Msa0580730:CDS
CAATTGATGGAGCAAACTTG+GGG 0.644212 4_1:-85937553 Msa0580730:CDS
CAAATTATCAAACCAAACAT+GGG 0.651382 4_1:-85937932 Msa0580730:exon
AAGATATGTATGTTGGTGTG+TGG 0.694182 4_1:+85938009 None:intergenic
GAAACCGTTGAGGGAAATGG+TGG 0.710996 4_1:-85937791 Msa0580730:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! AATCAATAAATTAATACAAA+TGG - chr4_1:85937830-85937849 Msa0580730:CDS 10.0%
!! AATCAATAAATTAATACAAA+TGG - chr4_1:85937830-85937849 Msa0580730:CDS 10.0%
!! AAGAAAAAACTTTATTACAT+TGG + chr4_1:85937748-85937767 None:intergenic 15.0%
!!! AATAAAGTTTTTTCTTTGTA+AGG - chr4_1:85937752-85937771 Msa0580730:intron 15.0%
!! AAAACAACATAAATCTTATT+TGG + chr4_1:85937865-85937884 None:intergenic 15.0%
!! ACAACAACATAAATATTATT+TGG + chr4_1:85937895-85937914 None:intergenic 15.0%
!! AAGAAAAAACTTTATTACAT+TGG + chr4_1:85937748-85937767 None:intergenic 15.0%
!!! AATAAAGTTTTTTCTTTGTA+AGG - chr4_1:85937752-85937771 Msa0580730:intron 15.0%
!! AAAACAACATAAATCTTATT+TGG + chr4_1:85937865-85937884 None:intergenic 15.0%
!! ACAACAACATAAATATTATT+TGG + chr4_1:85937895-85937914 None:intergenic 15.0%
!! TGTTGTTAAGTTTATTTACT+TGG - chr4_1:85937946-85937965 Msa0580730:five_prime_UTR 20.0%
!! TGTTGTTAAGTTTATTTACT+TGG - chr4_1:85937946-85937965 Msa0580730:five_prime_UTR 20.0%
! CAAATTATCAAACCAAACAT+GGG - chr4_1:85937021-85937040 Msa0580730:three_prime_UTR 25.0%
! TATAACTATAGTATAGTTGG+AGG - chr4_1:85937423-85937442 Msa0580730:CDS 25.0%
!!! TCCTTTGGTTTGATATTTAA+CGG + chr4_1:85937535-85937554 None:intergenic 25.0%
!!! TAGATGGATTAGTTGTAATT+AGG + chr4_1:85937648-85937667 None:intergenic 25.0%
! CAAATTATCAAACCAAACAT+GGG - chr4_1:85937021-85937040 Msa0580730:three_prime_UTR 25.0%
! TATAACTATAGTATAGTTGG+AGG - chr4_1:85937423-85937442 Msa0580730:CDS 25.0%
!!! TCCTTTGGTTTGATATTTAA+CGG + chr4_1:85937535-85937554 None:intergenic 25.0%
!!! TAGATGGATTAGTTGTAATT+AGG + chr4_1:85937648-85937667 None:intergenic 25.0%
TGAGAACAAGATATGTATGT+TGG + chr4_1:85936954-85936973 None:intergenic 30.0%
!! TTGGTGATGAAGTTGTAAAA+GGG + chr4_1:85937004-85937023 None:intergenic 30.0%
!! TTTGGTGATGAAGTTGTAAA+AGG + chr4_1:85937005-85937024 None:intergenic 30.0%
!! CCATGTTTGGTTTGATAATT+TGG + chr4_1:85937023-85937042 None:intergenic 30.0%
CCAAATTATCAAACCAAACA+TGG - chr4_1:85937020-85937039 Msa0580730:three_prime_UTR 30.0%
!! TGTCGCACATGTTTTTAATA+TGG - chr4_1:85937280-85937299 Msa0580730:three_prime_UTR 30.0%
GGGTATAACTATAGTATAGT+TGG - chr4_1:85937420-85937439 Msa0580730:CDS 30.0%
! GATATCATTTGAAGCGAAAT+TGG - chr4_1:85937473-85937492 Msa0580730:CDS 30.0%
ACCGTTAAATATCAAACCAA+AGG - chr4_1:85937531-85937550 Msa0580730:CDS 30.0%
!! GGATTAGTTGTAATTAGGAT+TGG + chr4_1:85937643-85937662 None:intergenic 30.0%
ACAGATGATAGTAGATTAGT+AGG + chr4_1:85937680-85937699 None:intergenic 30.0%
!!! TTTTTTCTTGCTTTTGAACC+AGG - chr4_1:85937775-85937794 Msa0580730:CDS 30.0%
!! TTTTTGCTTTGAACAACAAC+CGG + chr4_1:85938009-85938028 None:intergenic 30.0%
GTTGTTCAAAGCAAAAATCA+AGG - chr4_1:85938012-85938031 Msa0580730:five_prime_UTR 30.0%
TGAGAACAAGATATGTATGT+TGG + chr4_1:85936954-85936973 None:intergenic 30.0%
!! TTGGTGATGAAGTTGTAAAA+GGG + chr4_1:85937004-85937023 None:intergenic 30.0%
!! TTTGGTGATGAAGTTGTAAA+AGG + chr4_1:85937005-85937024 None:intergenic 30.0%
!! CCATGTTTGGTTTGATAATT+TGG + chr4_1:85937023-85937042 None:intergenic 30.0%
CCAAATTATCAAACCAAACA+TGG - chr4_1:85937020-85937039 Msa0580730:three_prime_UTR 30.0%
!! TGTCGCACATGTTTTTAATA+TGG - chr4_1:85937280-85937299 Msa0580730:three_prime_UTR 30.0%
GGGTATAACTATAGTATAGT+TGG - chr4_1:85937420-85937439 Msa0580730:CDS 30.0%
! GATATCATTTGAAGCGAAAT+TGG - chr4_1:85937473-85937492 Msa0580730:CDS 30.0%
ACCGTTAAATATCAAACCAA+AGG - chr4_1:85937531-85937550 Msa0580730:CDS 30.0%
!! GGATTAGTTGTAATTAGGAT+TGG + chr4_1:85937643-85937662 None:intergenic 30.0%
ACAGATGATAGTAGATTAGT+AGG + chr4_1:85937680-85937699 None:intergenic 30.0%
!!! TTTTTTCTTGCTTTTGAACC+AGG - chr4_1:85937775-85937794 Msa0580730:CDS 30.0%
!! TTTTTGCTTTGAACAACAAC+CGG + chr4_1:85938009-85938028 None:intergenic 30.0%
GTTGTTCAAAGCAAAAATCA+AGG - chr4_1:85938012-85938031 Msa0580730:five_prime_UTR 30.0%
AAGATATGTATGTTGGTGTG+TGG + chr4_1:85936947-85936966 None:intergenic 35.0%
AATGTGAATACACCCATGTT+TGG + chr4_1:85937036-85937055 None:intergenic 35.0%
ACCAACATAAATATGTACGC+AGG - chr4_1:85937342-85937361 Msa0580730:CDS 35.0%
TCCTGCGTACATATTTATGT+TGG + chr4_1:85937346-85937365 None:intergenic 35.0%
AACATAAATATGTACGCAGG+AGG - chr4_1:85937345-85937364 Msa0580730:CDS 35.0%
ACATAAATATGTACGCAGGA+GGG - chr4_1:85937346-85937365 Msa0580730:CDS 35.0%
CACCAAATTGATGCAATTGA+TGG - chr4_1:85937387-85937406 Msa0580730:CDS 35.0%
CTCCATCAATTGCATCAATT+TGG + chr4_1:85937392-85937411 None:intergenic 35.0%
TATAGTATAGTTGGAGGAGT+AGG - chr4_1:85937429-85937448 Msa0580730:CDS 35.0%
ATAGTATAGTTGGAGGAGTA+GGG - chr4_1:85937430-85937449 Msa0580730:CDS 35.0%
! TTTGAAGCGAAATTGGTTGA+AGG - chr4_1:85937480-85937499 Msa0580730:CDS 35.0%
AAATTGGTTGAAGGTCCAAA+TGG - chr4_1:85937489-85937508 Msa0580730:CDS 35.0%
AGATGCTAAGCCAATTGAAA+AGG - chr4_1:85937554-85937573 Msa0580730:CDS 35.0%
AGTAGATTAGTAGGTAGACT+TGG + chr4_1:85937671-85937690 None:intergenic 35.0%
TTGAGAGCAAAATCCATTAC+CGG - chr4_1:85937987-85938006 Msa0580730:five_prime_UTR 35.0%
AAGATATGTATGTTGGTGTG+TGG + chr4_1:85936947-85936966 None:intergenic 35.0%
AATGTGAATACACCCATGTT+TGG + chr4_1:85937036-85937055 None:intergenic 35.0%
ACCAACATAAATATGTACGC+AGG - chr4_1:85937342-85937361 Msa0580730:CDS 35.0%
TCCTGCGTACATATTTATGT+TGG + chr4_1:85937346-85937365 None:intergenic 35.0%
AACATAAATATGTACGCAGG+AGG - chr4_1:85937345-85937364 Msa0580730:CDS 35.0%
ACATAAATATGTACGCAGGA+GGG - chr4_1:85937346-85937365 Msa0580730:CDS 35.0%
CACCAAATTGATGCAATTGA+TGG - chr4_1:85937387-85937406 Msa0580730:CDS 35.0%
CTCCATCAATTGCATCAATT+TGG + chr4_1:85937392-85937411 None:intergenic 35.0%
TATAGTATAGTTGGAGGAGT+AGG - chr4_1:85937429-85937448 Msa0580730:CDS 35.0%
ATAGTATAGTTGGAGGAGTA+GGG - chr4_1:85937430-85937449 Msa0580730:CDS 35.0%
! TTTGAAGCGAAATTGGTTGA+AGG - chr4_1:85937480-85937499 Msa0580730:CDS 35.0%
AAATTGGTTGAAGGTCCAAA+TGG - chr4_1:85937489-85937508 Msa0580730:CDS 35.0%
AGATGCTAAGCCAATTGAAA+AGG - chr4_1:85937554-85937573 Msa0580730:CDS 35.0%
AGTAGATTAGTAGGTAGACT+TGG + chr4_1:85937671-85937690 None:intergenic 35.0%
TTGAGAGCAAAATCCATTAC+CGG - chr4_1:85937987-85938006 Msa0580730:five_prime_UTR 35.0%
! AGAGCTTTGTAAAGCTTAGC+AGG + chr4_1:85937087-85937106 None:intergenic 40.0%
GGATGATATCATCAGAGTCA+TGG + chr4_1:85937113-85937132 None:intergenic 40.0%
! TGATGGCATCAACAGCTTTT+GGG + chr4_1:85937134-85937153 None:intergenic 40.0%
!! TTGATGGCATCAACAGCTTT+TGG + chr4_1:85937135-85937154 None:intergenic 40.0%
AACGGTTTCAACACTCTTGA+TGG + chr4_1:85937151-85937170 None:intergenic 40.0%
!! AAGAGTGTTGAAACCGTTGA+GGG - chr4_1:85937153-85937172 Msa0580730:three_prime_UTR 40.0%
AACGAAAGTGAGCTTCTTGA+CGG + chr4_1:85937196-85937215 None:intergenic 40.0%
AAGAAGCTCACTTTCGTTGA+GGG - chr4_1:85937198-85937217 Msa0580730:three_prime_UTR 40.0%
!! AATTTGGTGCAACACGTACA+AGG + chr4_1:85937376-85937395 None:intergenic 40.0%
TGCAATTGATGGAGCAAACT+TGG - chr4_1:85937398-85937417 Msa0580730:CDS 40.0%
GCAATTGATGGAGCAAACTT+GGG - chr4_1:85937399-85937418 Msa0580730:CDS 40.0%
CAATTGATGGAGCAAACTTG+GGG - chr4_1:85937400-85937419 Msa0580730:CDS 40.0%
! GATATCTTTTCAACCGTGTC+TGG + chr4_1:85937459-85937478 None:intergenic 40.0%
! AATTGGCTTAGCATCTCCTT+TGG + chr4_1:85937550-85937569 None:intergenic 40.0%
TGCTAAGCCAATTGAAAAGG+AGG - chr4_1:85937557-85937576 Msa0580730:CDS 40.0%
! TCCTCAACCTCCTTTTCAAT+TGG + chr4_1:85937567-85937586 None:intergenic 40.0%
ATTGAAAAGGAGGTTGAGGA+AGG - chr4_1:85937567-85937586 Msa0580730:CDS 40.0%
! AAAGGGTGATGCTCTTTTCA+AGG - chr4_1:85937596-85937615 Msa0580730:intron 40.0%
! TAGTAGGTAGACTTGGTAGA+TGG + chr4_1:85937664-85937683 None:intergenic 40.0%
! TGCTTTTGAACCAGGAGATT+TGG - chr4_1:85937783-85937802 Msa0580730:CDS 40.0%
CAACAAAACACCAAATCTCC+TGG + chr4_1:85937796-85937815 None:intergenic 40.0%
CTTTGAACAACAACCGGTAA+TGG + chr4_1:85938003-85938022 None:intergenic 40.0%
! AGAGCTTTGTAAAGCTTAGC+AGG + chr4_1:85937087-85937106 None:intergenic 40.0%
GGATGATATCATCAGAGTCA+TGG + chr4_1:85937113-85937132 None:intergenic 40.0%
! TGATGGCATCAACAGCTTTT+GGG + chr4_1:85937134-85937153 None:intergenic 40.0%
!! TTGATGGCATCAACAGCTTT+TGG + chr4_1:85937135-85937154 None:intergenic 40.0%
AACGGTTTCAACACTCTTGA+TGG + chr4_1:85937151-85937170 None:intergenic 40.0%
!! AAGAGTGTTGAAACCGTTGA+GGG - chr4_1:85937153-85937172 Msa0580730:three_prime_UTR 40.0%
AACGAAAGTGAGCTTCTTGA+CGG + chr4_1:85937196-85937215 None:intergenic 40.0%
AAGAAGCTCACTTTCGTTGA+GGG - chr4_1:85937198-85937217 Msa0580730:three_prime_UTR 40.0%
!! AATTTGGTGCAACACGTACA+AGG + chr4_1:85937376-85937395 None:intergenic 40.0%
TGCAATTGATGGAGCAAACT+TGG - chr4_1:85937398-85937417 Msa0580730:CDS 40.0%
GCAATTGATGGAGCAAACTT+GGG - chr4_1:85937399-85937418 Msa0580730:CDS 40.0%
CAATTGATGGAGCAAACTTG+GGG - chr4_1:85937400-85937419 Msa0580730:CDS 40.0%
! GATATCTTTTCAACCGTGTC+TGG + chr4_1:85937459-85937478 None:intergenic 40.0%
! AATTGGCTTAGCATCTCCTT+TGG + chr4_1:85937550-85937569 None:intergenic 40.0%
TGCTAAGCCAATTGAAAAGG+AGG - chr4_1:85937557-85937576 Msa0580730:CDS 40.0%
! TCCTCAACCTCCTTTTCAAT+TGG + chr4_1:85937567-85937586 None:intergenic 40.0%
ATTGAAAAGGAGGTTGAGGA+AGG - chr4_1:85937567-85937586 Msa0580730:CDS 40.0%
! AAAGGGTGATGCTCTTTTCA+AGG - chr4_1:85937596-85937615 Msa0580730:intron 40.0%
! TAGTAGGTAGACTTGGTAGA+TGG + chr4_1:85937664-85937683 None:intergenic 40.0%
! TGCTTTTGAACCAGGAGATT+TGG - chr4_1:85937783-85937802 Msa0580730:CDS 40.0%
CAACAAAACACCAAATCTCC+TGG + chr4_1:85937796-85937815 None:intergenic 40.0%
CTTTGAACAACAACCGGTAA+TGG + chr4_1:85938003-85938022 None:intergenic 40.0%
TATGTATGTTGGTGTGTGGC+TGG + chr4_1:85936943-85936962 None:intergenic 45.0%
!! CAAGAGTGTTGAAACCGTTG+AGG - chr4_1:85937152-85937171 Msa0580730:three_prime_UTR 45.0%
GTTGAAACCGTTGAGGGAAA+TGG - chr4_1:85937159-85937178 Msa0580730:three_prime_UTR 45.0%
CAAGAAGCTCACTTTCGTTG+AGG - chr4_1:85937197-85937216 Msa0580730:three_prime_UTR 45.0%
TTGGTTGAAGGTCCAAATGG+AGG - chr4_1:85937492-85937511 Msa0580730:CDS 45.0%
TGGTTGAAGGTCCAAATGGA+GGG - chr4_1:85937493-85937512 Msa0580730:CDS 45.0%
GTCCAAATGGAGGGTCTATT+GGG - chr4_1:85937502-85937521 Msa0580730:CDS 45.0%
TTCCCAATAGACCCTCCATT+TGG + chr4_1:85937507-85937526 None:intergenic 45.0%
GCCAATTGAAAAGGAGGTTG+AGG - chr4_1:85937563-85937582 Msa0580730:CDS 45.0%
!! TGCTCTTTTCAAGGCCATTG+AGG - chr4_1:85937605-85937624 Msa0580730:intron 45.0%
!! GCTCTTTTCAAGGCCATTGA+GGG - chr4_1:85937606-85937625 Msa0580730:intron 45.0%
! GGCCATTGAGGGTTATGTTT+TGG - chr4_1:85937617-85937636 Msa0580730:intron 45.0%
GGCCAAAACATAACCCTCAA+TGG + chr4_1:85937622-85937641 None:intergenic 45.0%
TATGTATGTTGGTGTGTGGC+TGG + chr4_1:85936943-85936962 None:intergenic 45.0%
!! CAAGAGTGTTGAAACCGTTG+AGG - chr4_1:85937152-85937171 Msa0580730:three_prime_UTR 45.0%
GTTGAAACCGTTGAGGGAAA+TGG - chr4_1:85937159-85937178 Msa0580730:three_prime_UTR 45.0%
CAAGAAGCTCACTTTCGTTG+AGG - chr4_1:85937197-85937216 Msa0580730:three_prime_UTR 45.0%
TTGGTTGAAGGTCCAAATGG+AGG - chr4_1:85937492-85937511 Msa0580730:CDS 45.0%
TGGTTGAAGGTCCAAATGGA+GGG - chr4_1:85937493-85937512 Msa0580730:CDS 45.0%
GTCCAAATGGAGGGTCTATT+GGG - chr4_1:85937502-85937521 Msa0580730:CDS 45.0%
TTCCCAATAGACCCTCCATT+TGG + chr4_1:85937507-85937526 None:intergenic 45.0%
GCCAATTGAAAAGGAGGTTG+AGG - chr4_1:85937563-85937582 Msa0580730:CDS 45.0%
!! TGCTCTTTTCAAGGCCATTG+AGG - chr4_1:85937605-85937624 Msa0580730:intron 45.0%
!! GCTCTTTTCAAGGCCATTGA+GGG - chr4_1:85937606-85937625 Msa0580730:intron 45.0%
! GGCCATTGAGGGTTATGTTT+TGG - chr4_1:85937617-85937636 Msa0580730:intron 45.0%
GGCCAAAACATAACCCTCAA+TGG + chr4_1:85937622-85937641 None:intergenic 45.0%
CTTAGCAGGAGCAACTGTAG+AGG + chr4_1:85937073-85937092 None:intergenic 50.0%
GAAACCGTTGAGGGAAATGG+TGG - chr4_1:85937162-85937181 Msa0580730:three_prime_UTR 50.0%
AGGACCACCATTTCCCTCAA+CGG + chr4_1:85937169-85937188 None:intergenic 50.0%
GGTCCAAATGGAGGGTCTAT+TGG - chr4_1:85937501-85937520 Msa0580730:CDS 50.0%
AAAGGAGGTTGAGGAAGGCA+AGG - chr4_1:85937572-85937591 Msa0580730:CDS 50.0%
GTTGAGGAAGGCAAGGCAAA+GGG - chr4_1:85937579-85937598 Msa0580730:CDS 50.0%
CTTAGCAGGAGCAACTGTAG+AGG + chr4_1:85937073-85937092 None:intergenic 50.0%
GAAACCGTTGAGGGAAATGG+TGG - chr4_1:85937162-85937181 Msa0580730:three_prime_UTR 50.0%
AGGACCACCATTTCCCTCAA+CGG + chr4_1:85937169-85937188 None:intergenic 50.0%
GGTCCAAATGGAGGGTCTAT+TGG - chr4_1:85937501-85937520 Msa0580730:CDS 50.0%
AAAGGAGGTTGAGGAAGGCA+AGG - chr4_1:85937572-85937591 Msa0580730:CDS 50.0%
GTTGAGGAAGGCAAGGCAAA+GGG - chr4_1:85937579-85937598 Msa0580730:CDS 50.0%
AGCAGGAGCAACTGTAGAGG+TGG + chr4_1:85937070-85937089 None:intergenic 55.0%
GTTGAGGGAAATGGTGGTCC+TGG - chr4_1:85937168-85937187 Msa0580730:three_prime_UTR 55.0%
AGGAGTAGGGTTGCCAGACA+CGG - chr4_1:85937443-85937462 Msa0580730:CDS 55.0%
GGTTGAGGAAGGCAAGGCAA+AGG - chr4_1:85937578-85937597 Msa0580730:CDS 55.0%
AGCAGGAGCAACTGTAGAGG+TGG + chr4_1:85937070-85937089 None:intergenic 55.0%
GTTGAGGGAAATGGTGGTCC+TGG - chr4_1:85937168-85937187 Msa0580730:three_prime_UTR 55.0%
AGGAGTAGGGTTGCCAGACA+CGG - chr4_1:85937443-85937462 Msa0580730:CDS 55.0%
GGTTGAGGAAGGCAAGGCAA+AGG - chr4_1:85937578-85937597 Msa0580730:CDS 55.0%
GTGAGCTTCTTGACGGTGCC+AGG + chr4_1:85937189-85937208 None:intergenic 60.0%
GTGAGCTTCTTGACGGTGCC+AGG + chr4_1:85937189-85937208 None:intergenic 60.0%
Chromosome Type Strat End Strand Name
chr4_1 gene 85936937 85938038 85936937 ID=Msa0580730;Name=Msa0580730
chr4_1 mRNA 85936937 85938038 85936937 ID=Msa0580730-mRNA-1;Parent=Msa0580730;Name=Msa0580730-mRNA-1;_AED=0.07;_eAED=0.07;_QI=102|1|1|1|1|1|2|379|158
chr4_1 exon 85936937 85937611 85936937 ID=Msa0580730-mRNA-1:exon:31615;Parent=Msa0580730-mRNA-1
chr4_1 exon 85937756 85938038 85937756 ID=Msa0580730-mRNA-1:exon:31614;Parent=Msa0580730-mRNA-1
chr4_1 five_prime_UTR 85937937 85938038 85937937 ID=Msa0580730-mRNA-1:five_prime_utr;Parent=Msa0580730-mRNA-1
chr4_1 CDS 85937756 85937936 85937756 ID=Msa0580730-mRNA-1:cds;Parent=Msa0580730-mRNA-1
chr4_1 CDS 85937316 85937611 85937316 ID=Msa0580730-mRNA-1:cds;Parent=Msa0580730-mRNA-1
chr4_1 three_prime_UTR 85936937 85937315 85936937 ID=Msa0580730-mRNA-1:three_prime_utr;Parent=Msa0580730-mRNA-1
Gene Sequence

>Msa0580730

ATGGGTGTATTCACATTTGAGCAAGAAACCACCTCTACAGTTGCTCCTGCTAAGCTTTACAAAGCTCTTGTCCATGACTCTGATGATATCATCCCAAAAGCTGTTGATGCCATCAAGAGTGTTGAAACCGTTGAGGGAAATGGTGGTCCTGGCACCGTCAAGAAGCTCACTTTCGTTGAGGGAGGGCAAACCTTGTACGTGTTGCACCAAATTGATGCAATTGATGGAGCAAACTTGGGGTATAACTATAGTATAGTTGGAGGAGTAGGGTTGCCAGACACGGTTGAAAAGATATCATTTGAAGCGAAATTGGTTGAAGGTCCAAATGGAGGGTCTATTGGGAAGACGACCGTTAAATATCAAACCAAAGGAGATGCTAAGCCAATTGAAAAGGAGGTTGAGGAAGGCAAGGCAAAGGGTGATGCTCTTTTCAAGGCCATTGAGGGTTATGTTTTGGCCAATCCTAATTACAACTAA
Protein sequence

>Msa0580730

MGVFTFEQETTSTVAPAKLYKALVHDSDDIIPKAVDAIKSVETVEGNGGPGTVKKLTFVEGGQTLYVLHQIDAIDGANLGYNYSIVGGVGLPDTVEKISFEAKLVEGPNGGSIGKTTVKYQTKGDAKPIEKEVEEGKAKGDALFKAIEGYVLANPNYN*