AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa0138830

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0138830 MtrunA17_Chr4g0067951 97.468 158 4 0 1 158 1 158 2.68e-108 305
Msa0138830 MtrunA17_Chr6g0462311 96.203 158 6 0 1 158 1 158 4.08e-107 302
Msa0138830 MtrunA17_Chr6g0462291 84.049 163 21 1 1 158 1 163 1.48e-92 266
Msa0138830 MtrunA17_Chr2g0295064 74.051 158 41 0 1 158 68 225 2.60e-81 239
Msa0138830 MtrunA17_Chr2g0295021 72.611 157 43 0 1 157 1 157 5.38e-80 233
Msa0138830 MtrunA17_Chr2g0295001 72.611 157 43 0 1 157 1 157 5.38e-80 233
Msa0138830 MtrunA17_Chr2g0295051 70.064 157 47 0 1 157 1 157 7.62e-78 228
Msa0138830 MtrunA17_Chr2g0295041 68.354 158 50 0 1 158 1 158 1.47e-75 222
Msa0138830 MtrunA17_Chr2g0295141 63.694 157 57 0 1 157 1 157 1.35e-68 204
Msa0138830 MtrunA17_Chr2g0295104 64.331 157 56 0 1 157 1 157 2.52e-68 204
Msa0138830 MtrunA17_Chr2g0295091 64.331 157 56 0 1 157 1 157 8.50e-68 202
Msa0138830 MtrunA17_Chr2g0295124 64.968 157 55 0 1 157 1 157 8.66e-67 200
Msa0138830 MtrunA17_Chr2g0295081 54.140 157 72 0 1 157 1 157 1.81e-54 169
Msa0138830 MtrunA17_Chr4g0067921 45.963 161 82 4 1 158 1 159 2.51e-37 125
Msa0138830 MtrunA17_Chr2g0295211 37.179 156 97 1 1 155 1 156 2.68e-33 115
Msa0138830 MtrunA17_Chr4g0067841 36.943 157 98 1 1 156 1 157 1.02e-32 114
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score

Find 68 sgRNAs with CRISPR-Local

Find 166 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
ACAACAACATAAATATTATT+TGG 0.112159 1_3:-72057950 None:intergenic
CCATGTTTGGTTTGATAATT+TGG 0.145886 1_3:-72057078 None:intergenic
AAAACAACATAAATCTTATT+TGG 0.160723 1_3:-72057920 None:intergenic
CTCCATCAATTGCATCAATT+TGG 0.202998 1_3:-72057447 None:intergenic
TTGATGGCATCAACAGCTTT+TGG 0.227157 1_3:-72057190 None:intergenic
GATATCATTTGAAGCGAAAT+TGG 0.228714 1_3:+72057531 Msa0138830:CDS
TAGATGGATTAGTTGTAATT+AGG 0.259719 1_3:-72057703 None:intergenic
GGCCATTGAGGGTTATGTTT+TGG 0.293726 1_3:+72057675 Msa0138830:CDS
ATTTGGTGATGAAGTTCTAA+TGG 0.299038 1_3:-72057061 None:intergenic
AACGGTTTCAACACTCTTGA+TGG 0.328088 1_3:-72057206 None:intergenic
GGTCCAAATGGAGGGTCTAT+TGG 0.333504 1_3:+72057559 Msa0138830:CDS
TCCTTTGGTTTGATATTTAA+CGG 0.347214 1_3:-72057590 None:intergenic
TGTTGTTAAGTTTATTTACT+TGG 0.354046 1_3:+72058005 Msa0138830:three_prime_UTR
CACCAAATTGATGCAATTGA+TGG 0.359913 1_3:+72057445 Msa0138830:CDS
TAGTAGGTAGACTTGGTAGA+TGG 0.361526 1_3:-72057719 None:intergenic
CTTTGAACAACAACCGGTAA+TGG 0.364496 1_3:-72058059 None:intergenic
GTCCAAATGGAGGGTCTATT+GGG 0.390136 1_3:+72057560 Msa0138830:CDS
AATTGGCTTAGCATCTCCTT+TGG 0.398977 1_3:-72057605 None:intergenic
TATAGTATAGTTGGAGGAGT+AGG 0.400784 1_3:+72057487 Msa0138830:CDS
TGCAATTGATGGAGCAAACT+TGG 0.407405 1_3:+72057456 Msa0138830:CDS
TTTGGTGATGAAGTTCTAAT+GGG 0.409031 1_3:-72057060 None:intergenic
AATCAATAAATTAATACAAA+TGG 0.412812 1_3:+72057888 Msa0138830:three_prime_UTR
TTGAGAGCAAAATCCATTAC+CGG 0.415935 1_3:+72058046 Msa0138830:three_prime_UTR
TTCCCAATAGACCCTCCATT+TGG 0.416216 1_3:-72057562 None:intergenic
AATGTGAATACACCCATGTT+TGG 0.426195 1_3:-72057091 None:intergenic
AGAGCTTTGTAAAGCTTAGC+AGG 0.435032 1_3:-72057142 None:intergenic
AACGAAAGTGAGCTTCTTGA+CGG 0.435771 1_3:-72057251 None:intergenic
ACAGATGATAGTAGATTAGT+AGG 0.436784 1_3:-72057735 None:intergenic
AAGAAGCTCACTTTCGTTGA+GGG 0.454166 1_3:+72057256 Msa0138830:CDS
GGATTAGTTGTAATTAGGAT+TGG 0.464473 1_3:-72057698 None:intergenic
GTGAGCTTCTTGACGGTGCC+AGG 0.467117 1_3:-72057244 None:intergenic
CCAAATTATCAAACCAAACA+TGG 0.473191 1_3:+72057078 Msa0138830:exon
GGTTGAGGAAGGCAAGGCAA+AGG 0.481278 1_3:+72057636 Msa0138830:CDS
GTTGAAACCGTTGAGGGAAA+TGG 0.483004 1_3:+72057217 Msa0138830:CDS
TTTGAAGCGAAATTGGTTGA+AGG 0.487809 1_3:+72057538 Msa0138830:CDS
TTGTTCAAAGCAAAATATCA+AGG 0.488358 1_3:+72058072 Msa0138830:three_prime_UTR
GGGTATAACTATAGTATAGT+TGG 0.489142 1_3:+72057478 Msa0138830:CDS
ATAGTATAGTTGGAGGAGTA+GGG 0.491450 1_3:+72057488 Msa0138830:CDS
AGTAGATTAGTAGGTAGACT+TGG 0.500749 1_3:-72057726 None:intergenic
AGATGCTAAGCCAATTGAAA+AGG 0.515464 1_3:+72057612 Msa0138830:CDS
GTTGAGGGAAATGGTGGTCC+TGG 0.519714 1_3:+72057226 Msa0138830:CDS
GCCAATTGAAAAGGAGGTTG+AGG 0.520188 1_3:+72057621 Msa0138830:CDS
GGCCAAAACATAACCCTCAA+TGG 0.521409 1_3:-72057677 None:intergenic
CAAGAAGCTCACTTTCGTTG+AGG 0.534631 1_3:+72057255 Msa0138830:CDS
GCAATTGATGGAGCAAACTT+GGG 0.541306 1_3:+72057457 Msa0138830:CDS
GTTGAGGAAGGCAAGGCAAA+GGG 0.544771 1_3:+72057637 Msa0138830:CDS
AAGAGTGTTGAAACCGTTGA+GGG 0.546792 1_3:+72057211 Msa0138830:CDS
AATTTGGTGCAACACGTACA+AGG 0.554848 1_3:-72057431 None:intergenic
CAACAAAACACCAAATCTCC+TGG 0.558696 1_3:-72057851 None:intergenic
GGATGATATCATCAGAGTCA+TGG 0.567722 1_3:-72057168 None:intergenic
AAATTGGTTGAAGGTCCAAA+TGG 0.568255 1_3:+72057547 Msa0138830:CDS
AGGACCACCATTTCCCTCAA+CGG 0.572788 1_3:-72057224 None:intergenic
TATAACTATAGTATAGTTGG+AGG 0.584231 1_3:+72057481 Msa0138830:CDS
AGCAGGAGCAACTGTAGAGG+TGG 0.585421 1_3:-72057125 None:intergenic
CTTAGCAGGAGCAACTGTAG+AGG 0.591469 1_3:-72057128 None:intergenic
TGCTAAGCCAATTGAAAAGG+AGG 0.591628 1_3:+72057615 Msa0138830:CDS
ACCGTTAAATATCAAACCAA+AGG 0.596206 1_3:+72057589 Msa0138830:CDS
AGGAGTAGGGTTGCCAGACA+CGG 0.598102 1_3:+72057501 Msa0138830:CDS
TGGTTGAAGGTCCAAATGGA+GGG 0.601542 1_3:+72057551 Msa0138830:CDS
TTGGTTGAAGGTCCAAATGG+AGG 0.609517 1_3:+72057550 Msa0138830:CDS
ATTGAAAAGGAGGTTGAGGA+AGG 0.616285 1_3:+72057625 Msa0138830:CDS
CAAGAGTGTTGAAACCGTTG+AGG 0.622991 1_3:+72057210 Msa0138830:CDS
AAAGGAGGTTGAGGAAGGCA+AGG 0.629508 1_3:+72057630 Msa0138830:CDS
CAATTGATGGAGCAAACTTG+GGG 0.644212 1_3:+72057458 Msa0138830:CDS
CAAATTATCAAACCAAACAT+GGG 0.651382 1_3:+72057079 Msa0138830:exon
CCATATGCTCATAAGCCACA+CGG 0.666552 1_3:+72056985 None:intergenic
GAAACCGTTGAGGGAAATGG+TGG 0.710996 1_3:+72057220 Msa0138830:CDS
TGTATGTTAGTGTGTCCGTG+TGG 0.751770 1_3:-72057000 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! AATCAATAAATTAATACAAA+TGG + chr1_3:72057888-72057907 Msa0138830:three_prime_UTR 10.0%
!! AATCAATAAATTAATACAAA+TGG + chr1_3:72057888-72057907 Msa0138830:three_prime_UTR 10.0%
!! AAGAAAAAACTTTATTACAT+TGG - chr1_3:72057806-72057825 None:intergenic 15.0%
!!! AATAAAGTTTTTTCTTTGTA+AGG + chr1_3:72057810-72057829 Msa0138830:three_prime_UTR 15.0%
!! AAAACAACATAAATCTTATT+TGG - chr1_3:72057923-72057942 None:intergenic 15.0%
!! ACAACAACATAAATATTATT+TGG - chr1_3:72057953-72057972 None:intergenic 15.0%
!! AAGAAAAAACTTTATTACAT+TGG - chr1_3:72057806-72057825 None:intergenic 15.0%
!!! AATAAAGTTTTTTCTTTGTA+AGG + chr1_3:72057810-72057829 Msa0138830:three_prime_UTR 15.0%
!! AAAACAACATAAATCTTATT+TGG - chr1_3:72057923-72057942 None:intergenic 15.0%
!! ACAACAACATAAATATTATT+TGG - chr1_3:72057953-72057972 None:intergenic 15.0%
!! TGTTGTTAAGTTTATTTACT+TGG + chr1_3:72058005-72058024 Msa0138830:three_prime_UTR 20.0%
!! TGTTGTTAAGTTTATTTACT+TGG + chr1_3:72058005-72058024 Msa0138830:three_prime_UTR 20.0%
! CAAATTATCAAACCAAACAT+GGG + chr1_3:72057079-72057098 Msa0138830:exon 25.0%
! TATAACTATAGTATAGTTGG+AGG + chr1_3:72057481-72057500 Msa0138830:CDS 25.0%
!!! TCCTTTGGTTTGATATTTAA+CGG - chr1_3:72057593-72057612 None:intergenic 25.0%
!!! TAGATGGATTAGTTGTAATT+AGG - chr1_3:72057706-72057725 None:intergenic 25.0%
! TTGTTCAAAGCAAAATATCA+AGG + chr1_3:72058072-72058091 Msa0138830:three_prime_UTR 25.0%
! CAAATTATCAAACCAAACAT+GGG + chr1_3:72057079-72057098 Msa0138830:exon 25.0%
! TATAACTATAGTATAGTTGG+AGG + chr1_3:72057481-72057500 Msa0138830:CDS 25.0%
!!! TCCTTTGGTTTGATATTTAA+CGG - chr1_3:72057593-72057612 None:intergenic 25.0%
!!! TAGATGGATTAGTTGTAATT+AGG - chr1_3:72057706-72057725 None:intergenic 25.0%
! TTGTTCAAAGCAAAATATCA+AGG + chr1_3:72058072-72058091 Msa0138830:three_prime_UTR 25.0%
!! TTTGGTGATGAAGTTCTAAT+GGG - chr1_3:72057063-72057082 None:intergenic 30.0%
!! ATTTGGTGATGAAGTTCTAA+TGG - chr1_3:72057064-72057083 None:intergenic 30.0%
!! CCATGTTTGGTTTGATAATT+TGG - chr1_3:72057081-72057100 None:intergenic 30.0%
CCAAATTATCAAACCAAACA+TGG + chr1_3:72057078-72057097 Msa0138830:exon 30.0%
!! TGTCGCACATGTTTTTAATA+TGG + chr1_3:72057338-72057357 Msa0138830:intron 30.0%
GGGTATAACTATAGTATAGT+TGG + chr1_3:72057478-72057497 Msa0138830:CDS 30.0%
! GATATCATTTGAAGCGAAAT+TGG + chr1_3:72057531-72057550 Msa0138830:CDS 30.0%
ACCGTTAAATATCAAACCAA+AGG + chr1_3:72057589-72057608 Msa0138830:CDS 30.0%
!! GGATTAGTTGTAATTAGGAT+TGG - chr1_3:72057701-72057720 None:intergenic 30.0%
ACAGATGATAGTAGATTAGT+AGG - chr1_3:72057738-72057757 None:intergenic 30.0%
!!! TTTTTTCTTGCTTTTGAACC+AGG + chr1_3:72057833-72057852 Msa0138830:three_prime_UTR 30.0%
!! ATTTTGCTTTGAACAACAAC+CGG - chr1_3:72058068-72058087 None:intergenic 30.0%
!! TTTGGTGATGAAGTTCTAAT+GGG - chr1_3:72057063-72057082 None:intergenic 30.0%
!! ATTTGGTGATGAAGTTCTAA+TGG - chr1_3:72057064-72057083 None:intergenic 30.0%
!! CCATGTTTGGTTTGATAATT+TGG - chr1_3:72057081-72057100 None:intergenic 30.0%
CCAAATTATCAAACCAAACA+TGG + chr1_3:72057078-72057097 Msa0138830:exon 30.0%
!! TGTCGCACATGTTTTTAATA+TGG + chr1_3:72057338-72057357 Msa0138830:intron 30.0%
GGGTATAACTATAGTATAGT+TGG + chr1_3:72057478-72057497 Msa0138830:CDS 30.0%
! GATATCATTTGAAGCGAAAT+TGG + chr1_3:72057531-72057550 Msa0138830:CDS 30.0%
ACCGTTAAATATCAAACCAA+AGG + chr1_3:72057589-72057608 Msa0138830:CDS 30.0%
!! GGATTAGTTGTAATTAGGAT+TGG - chr1_3:72057701-72057720 None:intergenic 30.0%
ACAGATGATAGTAGATTAGT+AGG - chr1_3:72057738-72057757 None:intergenic 30.0%
!!! TTTTTTCTTGCTTTTGAACC+AGG + chr1_3:72057833-72057852 Msa0138830:three_prime_UTR 30.0%
!! ATTTTGCTTTGAACAACAAC+CGG - chr1_3:72058068-72058087 None:intergenic 30.0%
AATGTGAATACACCCATGTT+TGG - chr1_3:72057094-72057113 None:intergenic 35.0%
ACCAACATAAATATGTACGC+AGG + chr1_3:72057400-72057419 Msa0138830:intron 35.0%
TCCTGCGTACATATTTATGT+TGG - chr1_3:72057404-72057423 None:intergenic 35.0%
AACATAAATATGTACGCAGG+AGG + chr1_3:72057403-72057422 Msa0138830:intron 35.0%
ACATAAATATGTACGCAGGA+GGG + chr1_3:72057404-72057423 Msa0138830:intron 35.0%
CACCAAATTGATGCAATTGA+TGG + chr1_3:72057445-72057464 Msa0138830:CDS 35.0%
CTCCATCAATTGCATCAATT+TGG - chr1_3:72057450-72057469 None:intergenic 35.0%
TATAGTATAGTTGGAGGAGT+AGG + chr1_3:72057487-72057506 Msa0138830:CDS 35.0%
ATAGTATAGTTGGAGGAGTA+GGG + chr1_3:72057488-72057507 Msa0138830:CDS 35.0%
! TTTGAAGCGAAATTGGTTGA+AGG + chr1_3:72057538-72057557 Msa0138830:CDS 35.0%
AAATTGGTTGAAGGTCCAAA+TGG + chr1_3:72057547-72057566 Msa0138830:CDS 35.0%
AGATGCTAAGCCAATTGAAA+AGG + chr1_3:72057612-72057631 Msa0138830:CDS 35.0%
AGTAGATTAGTAGGTAGACT+TGG - chr1_3:72057729-72057748 None:intergenic 35.0%
TTGAGAGCAAAATCCATTAC+CGG + chr1_3:72058046-72058065 Msa0138830:three_prime_UTR 35.0%
AATGTGAATACACCCATGTT+TGG - chr1_3:72057094-72057113 None:intergenic 35.0%
ACCAACATAAATATGTACGC+AGG + chr1_3:72057400-72057419 Msa0138830:intron 35.0%
TCCTGCGTACATATTTATGT+TGG - chr1_3:72057404-72057423 None:intergenic 35.0%
AACATAAATATGTACGCAGG+AGG + chr1_3:72057403-72057422 Msa0138830:intron 35.0%
ACATAAATATGTACGCAGGA+GGG + chr1_3:72057404-72057423 Msa0138830:intron 35.0%
CACCAAATTGATGCAATTGA+TGG + chr1_3:72057445-72057464 Msa0138830:CDS 35.0%
CTCCATCAATTGCATCAATT+TGG - chr1_3:72057450-72057469 None:intergenic 35.0%
TATAGTATAGTTGGAGGAGT+AGG + chr1_3:72057487-72057506 Msa0138830:CDS 35.0%
ATAGTATAGTTGGAGGAGTA+GGG + chr1_3:72057488-72057507 Msa0138830:CDS 35.0%
! TTTGAAGCGAAATTGGTTGA+AGG + chr1_3:72057538-72057557 Msa0138830:CDS 35.0%
AAATTGGTTGAAGGTCCAAA+TGG + chr1_3:72057547-72057566 Msa0138830:CDS 35.0%
AGATGCTAAGCCAATTGAAA+AGG + chr1_3:72057612-72057631 Msa0138830:CDS 35.0%
AGTAGATTAGTAGGTAGACT+TGG - chr1_3:72057729-72057748 None:intergenic 35.0%
TTGAGAGCAAAATCCATTAC+CGG + chr1_3:72058046-72058065 Msa0138830:three_prime_UTR 35.0%
! AGAGCTTTGTAAAGCTTAGC+AGG - chr1_3:72057145-72057164 None:intergenic 40.0%
GGATGATATCATCAGAGTCA+TGG - chr1_3:72057171-72057190 None:intergenic 40.0%
! TGATGGCATCAACAGCTTTT+GGG - chr1_3:72057192-72057211 None:intergenic 40.0%
!! TTGATGGCATCAACAGCTTT+TGG - chr1_3:72057193-72057212 None:intergenic 40.0%
AACGGTTTCAACACTCTTGA+TGG - chr1_3:72057209-72057228 None:intergenic 40.0%
!! AAGAGTGTTGAAACCGTTGA+GGG + chr1_3:72057211-72057230 Msa0138830:CDS 40.0%
AACGAAAGTGAGCTTCTTGA+CGG - chr1_3:72057254-72057273 None:intergenic 40.0%
AAGAAGCTCACTTTCGTTGA+GGG + chr1_3:72057256-72057275 Msa0138830:CDS 40.0%
!! AATTTGGTGCAACACGTACA+AGG - chr1_3:72057434-72057453 None:intergenic 40.0%
TGCAATTGATGGAGCAAACT+TGG + chr1_3:72057456-72057475 Msa0138830:CDS 40.0%
GCAATTGATGGAGCAAACTT+GGG + chr1_3:72057457-72057476 Msa0138830:CDS 40.0%
CAATTGATGGAGCAAACTTG+GGG + chr1_3:72057458-72057477 Msa0138830:CDS 40.0%
! GATATCTTTTCAACCGTGTC+TGG - chr1_3:72057517-72057536 None:intergenic 40.0%
! AATTGGCTTAGCATCTCCTT+TGG - chr1_3:72057608-72057627 None:intergenic 40.0%
TGCTAAGCCAATTGAAAAGG+AGG + chr1_3:72057615-72057634 Msa0138830:CDS 40.0%
! TCCTCAACCTCCTTTTCAAT+TGG - chr1_3:72057625-72057644 None:intergenic 40.0%
ATTGAAAAGGAGGTTGAGGA+AGG + chr1_3:72057625-72057644 Msa0138830:CDS 40.0%
! AAAGGGTGATGCTCTTTTCA+AGG + chr1_3:72057654-72057673 Msa0138830:CDS 40.0%
! TAGTAGGTAGACTTGGTAGA+TGG - chr1_3:72057722-72057741 None:intergenic 40.0%
! TGCTTTTGAACCAGGAGATT+TGG + chr1_3:72057841-72057860 Msa0138830:three_prime_UTR 40.0%
CAACAAAACACCAAATCTCC+TGG - chr1_3:72057854-72057873 None:intergenic 40.0%
CTTTGAACAACAACCGGTAA+TGG - chr1_3:72058062-72058081 None:intergenic 40.0%
! AGAGCTTTGTAAAGCTTAGC+AGG - chr1_3:72057145-72057164 None:intergenic 40.0%
GGATGATATCATCAGAGTCA+TGG - chr1_3:72057171-72057190 None:intergenic 40.0%
! TGATGGCATCAACAGCTTTT+GGG - chr1_3:72057192-72057211 None:intergenic 40.0%
!! TTGATGGCATCAACAGCTTT+TGG - chr1_3:72057193-72057212 None:intergenic 40.0%
AACGGTTTCAACACTCTTGA+TGG - chr1_3:72057209-72057228 None:intergenic 40.0%
!! AAGAGTGTTGAAACCGTTGA+GGG + chr1_3:72057211-72057230 Msa0138830:CDS 40.0%
AACGAAAGTGAGCTTCTTGA+CGG - chr1_3:72057254-72057273 None:intergenic 40.0%
AAGAAGCTCACTTTCGTTGA+GGG + chr1_3:72057256-72057275 Msa0138830:CDS 40.0%
!! AATTTGGTGCAACACGTACA+AGG - chr1_3:72057434-72057453 None:intergenic 40.0%
TGCAATTGATGGAGCAAACT+TGG + chr1_3:72057456-72057475 Msa0138830:CDS 40.0%
GCAATTGATGGAGCAAACTT+GGG + chr1_3:72057457-72057476 Msa0138830:CDS 40.0%
CAATTGATGGAGCAAACTTG+GGG + chr1_3:72057458-72057477 Msa0138830:CDS 40.0%
! GATATCTTTTCAACCGTGTC+TGG - chr1_3:72057517-72057536 None:intergenic 40.0%
! AATTGGCTTAGCATCTCCTT+TGG - chr1_3:72057608-72057627 None:intergenic 40.0%
TGCTAAGCCAATTGAAAAGG+AGG + chr1_3:72057615-72057634 Msa0138830:CDS 40.0%
! TCCTCAACCTCCTTTTCAAT+TGG - chr1_3:72057625-72057644 None:intergenic 40.0%
ATTGAAAAGGAGGTTGAGGA+AGG + chr1_3:72057625-72057644 Msa0138830:CDS 40.0%
! AAAGGGTGATGCTCTTTTCA+AGG + chr1_3:72057654-72057673 Msa0138830:CDS 40.0%
! TAGTAGGTAGACTTGGTAGA+TGG - chr1_3:72057722-72057741 None:intergenic 40.0%
! TGCTTTTGAACCAGGAGATT+TGG + chr1_3:72057841-72057860 Msa0138830:three_prime_UTR 40.0%
CAACAAAACACCAAATCTCC+TGG - chr1_3:72057854-72057873 None:intergenic 40.0%
CTTTGAACAACAACCGGTAA+TGG - chr1_3:72058062-72058081 None:intergenic 40.0%
TGTATGTTAGTGTGTCCGTG+TGG - chr1_3:72057003-72057022 None:intergenic 45.0%
!! CAAGAGTGTTGAAACCGTTG+AGG + chr1_3:72057210-72057229 Msa0138830:CDS 45.0%
GTTGAAACCGTTGAGGGAAA+TGG + chr1_3:72057217-72057236 Msa0138830:CDS 45.0%
CAAGAAGCTCACTTTCGTTG+AGG + chr1_3:72057255-72057274 Msa0138830:CDS 45.0%
TTGGTTGAAGGTCCAAATGG+AGG + chr1_3:72057550-72057569 Msa0138830:CDS 45.0%
TGGTTGAAGGTCCAAATGGA+GGG + chr1_3:72057551-72057570 Msa0138830:CDS 45.0%
GTCCAAATGGAGGGTCTATT+GGG + chr1_3:72057560-72057579 Msa0138830:CDS 45.0%
TTCCCAATAGACCCTCCATT+TGG - chr1_3:72057565-72057584 None:intergenic 45.0%
GCCAATTGAAAAGGAGGTTG+AGG + chr1_3:72057621-72057640 Msa0138830:CDS 45.0%
!! TGCTCTTTTCAAGGCCATTG+AGG + chr1_3:72057663-72057682 Msa0138830:CDS 45.0%
!! GCTCTTTTCAAGGCCATTGA+GGG + chr1_3:72057664-72057683 Msa0138830:CDS 45.0%
! GGCCATTGAGGGTTATGTTT+TGG + chr1_3:72057675-72057694 Msa0138830:CDS 45.0%
GGCCAAAACATAACCCTCAA+TGG - chr1_3:72057680-72057699 None:intergenic 45.0%
TGTATGTTAGTGTGTCCGTG+TGG - chr1_3:72057003-72057022 None:intergenic 45.0%
!! CAAGAGTGTTGAAACCGTTG+AGG + chr1_3:72057210-72057229 Msa0138830:CDS 45.0%
GTTGAAACCGTTGAGGGAAA+TGG + chr1_3:72057217-72057236 Msa0138830:CDS 45.0%
CAAGAAGCTCACTTTCGTTG+AGG + chr1_3:72057255-72057274 Msa0138830:CDS 45.0%
TTGGTTGAAGGTCCAAATGG+AGG + chr1_3:72057550-72057569 Msa0138830:CDS 45.0%
TGGTTGAAGGTCCAAATGGA+GGG + chr1_3:72057551-72057570 Msa0138830:CDS 45.0%
GTCCAAATGGAGGGTCTATT+GGG + chr1_3:72057560-72057579 Msa0138830:CDS 45.0%
TTCCCAATAGACCCTCCATT+TGG - chr1_3:72057565-72057584 None:intergenic 45.0%
GCCAATTGAAAAGGAGGTTG+AGG + chr1_3:72057621-72057640 Msa0138830:CDS 45.0%
!! TGCTCTTTTCAAGGCCATTG+AGG + chr1_3:72057663-72057682 Msa0138830:CDS 45.0%
!! GCTCTTTTCAAGGCCATTGA+GGG + chr1_3:72057664-72057683 Msa0138830:CDS 45.0%
! GGCCATTGAGGGTTATGTTT+TGG + chr1_3:72057675-72057694 Msa0138830:CDS 45.0%
GGCCAAAACATAACCCTCAA+TGG - chr1_3:72057680-72057699 None:intergenic 45.0%
CTTAGCAGGAGCAACTGTAG+AGG - chr1_3:72057131-72057150 None:intergenic 50.0%
GAAACCGTTGAGGGAAATGG+TGG + chr1_3:72057220-72057239 Msa0138830:CDS 50.0%
AGGACCACCATTTCCCTCAA+CGG - chr1_3:72057227-72057246 None:intergenic 50.0%
GGTCCAAATGGAGGGTCTAT+TGG + chr1_3:72057559-72057578 Msa0138830:CDS 50.0%
AAAGGAGGTTGAGGAAGGCA+AGG + chr1_3:72057630-72057649 Msa0138830:CDS 50.0%
GTTGAGGAAGGCAAGGCAAA+GGG + chr1_3:72057637-72057656 Msa0138830:CDS 50.0%
CTTAGCAGGAGCAACTGTAG+AGG - chr1_3:72057131-72057150 None:intergenic 50.0%
GAAACCGTTGAGGGAAATGG+TGG + chr1_3:72057220-72057239 Msa0138830:CDS 50.0%
AGGACCACCATTTCCCTCAA+CGG - chr1_3:72057227-72057246 None:intergenic 50.0%
GGTCCAAATGGAGGGTCTAT+TGG + chr1_3:72057559-72057578 Msa0138830:CDS 50.0%
AAAGGAGGTTGAGGAAGGCA+AGG + chr1_3:72057630-72057649 Msa0138830:CDS 50.0%
GTTGAGGAAGGCAAGGCAAA+GGG + chr1_3:72057637-72057656 Msa0138830:CDS 50.0%
AGCAGGAGCAACTGTAGAGG+TGG - chr1_3:72057128-72057147 None:intergenic 55.0%
GTTGAGGGAAATGGTGGTCC+TGG + chr1_3:72057226-72057245 Msa0138830:CDS 55.0%
AGGAGTAGGGTTGCCAGACA+CGG + chr1_3:72057501-72057520 Msa0138830:CDS 55.0%
GGTTGAGGAAGGCAAGGCAA+AGG + chr1_3:72057636-72057655 Msa0138830:CDS 55.0%
AGCAGGAGCAACTGTAGAGG+TGG - chr1_3:72057128-72057147 None:intergenic 55.0%
GTTGAGGGAAATGGTGGTCC+TGG + chr1_3:72057226-72057245 Msa0138830:CDS 55.0%
AGGAGTAGGGTTGCCAGACA+CGG + chr1_3:72057501-72057520 Msa0138830:CDS 55.0%
GGTTGAGGAAGGCAAGGCAA+AGG + chr1_3:72057636-72057655 Msa0138830:CDS 55.0%
GTGAGCTTCTTGACGGTGCC+AGG - chr1_3:72057247-72057266 None:intergenic 60.0%
GTGAGCTTCTTGACGGTGCC+AGG - chr1_3:72057247-72057266 None:intergenic 60.0%
Chromosome Type Strat End Strand Name
chr1_3 gene 72056997 72058098 72056997 ID=Msa0138830;Name=Msa0138830
chr1_3 mRNA 72056997 72058098 72056997 ID=Msa0138830-mRNA-1;Parent=Msa0138830;Name=Msa0138830-mRNA-1;_AED=0.38;_eAED=0.38;_QI=100|1|1|1|1|1|2|381|158
chr1_3 exon 72056997 72057277 72056997 ID=Msa0138830-mRNA-1:exon:25111;Parent=Msa0138830-mRNA-1
chr1_3 exon 72057422 72058098 72057422 ID=Msa0138830-mRNA-1:exon:25112;Parent=Msa0138830-mRNA-1
chr1_3 five_prime_UTR 72056997 72057096 72056997 ID=Msa0138830-mRNA-1:five_prime_utr;Parent=Msa0138830-mRNA-1
chr1_3 CDS 72057097 72057277 72057097 ID=Msa0138830-mRNA-1:cds;Parent=Msa0138830-mRNA-1
chr1_3 CDS 72057422 72057717 72057422 ID=Msa0138830-mRNA-1:cds;Parent=Msa0138830-mRNA-1
chr1_3 three_prime_UTR 72057718 72058098 72057718 ID=Msa0138830-mRNA-1:three_prime_utr;Parent=Msa0138830-mRNA-1
Gene Sequence

>Msa0138830

ATGGGTGTATTCACATTTGAGCAAGAAACCACCTCTACAGTTGCTCCTGCTAAGCTTTACAAAGCTCTTGTCCATGACTCTGATGATATCATCCCAAAAGCTGTTGATGCCATCAAGAGTGTTGAAACCGTTGAGGGAAATGGTGGTCCTGGCACCGTCAAGAAGCTCACTTTCGTTGAGGGAGGGCAAACCTTGTACGTGTTGCACCAAATTGATGCAATTGATGGAGCAAACTTGGGGTATAACTATAGTATAGTTGGAGGAGTAGGGTTGCCAGACACGGTTGAAAAGATATCATTTGAAGCGAAATTGGTTGAAGGTCCAAATGGAGGGTCTATTGGGAAGACGACCGTTAAATATCAAACCAAAGGAGATGCTAAGCCAATTGAAAAGGAGGTTGAGGAAGGCAAGGCAAAGGGTGATGCTCTTTTCAAGGCCATTGAGGGTTATGTTTTGGCCAATCCTAATTACAACTAA
Protein sequence

>Msa0138830

MGVFTFEQETTSTVAPAKLYKALVHDSDDIIPKAVDAIKSVETVEGNGGPGTVKKLTFVEGGQTLYVLHQIDAIDGANLGYNYSIVGGVGLPDTVEKISFEAKLVEGPNGGSIGKTTVKYQTKGDAKPIEKEVEEGKAKGDALFKAIEGYVLANPNYN*