Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| Msa0203020 | XP_013463539.1 | 93.810 | 210 | 13 | 0 | 1 | 210 | 1 | 210 | 3.60e-140 | 400 |
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| Msa0203020 | sp|Q9ZRA4|AB19A_PRUPE | 72.596 | 208 | 56 | 1 | 3 | 209 | 1 | 208 | 3.13e-105 | 304 |
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| Msa0203020 | A0A072V680 | 93.810 | 210 | 13 | 0 | 1 | 210 | 1 | 210 | 1.72e-140 | 400 |
| Gene ID | Type | Classification |
|---|
| Gene ID | Type | Classification |
|---|
Co-expression Network
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| Msa0036430 | Msa0203020 | 0.800521 | 1.385981e-48 | -8.615850e-47 |
| Msa0084500 | Msa0203020 | 0.801196 | 1.009075e-48 | -8.615850e-47 |
| Msa0203010 | Msa0203020 | 0.913462 | 5.983969e-84 | -8.615850e-47 |
| Msa0203020 | Msa0203030 | 0.861973 | 7.041265e-64 | -8.615850e-47 |
| Msa0203020 | Msa0322830 | 0.809014 | 2.332869e-50 | -8.615850e-47 |
| Msa0203020 | Msa0322840 | 0.850873 | 1.281423e-60 | -8.615850e-47 |
| Msa0203020 | Msa0535040 | 0.807598 | 4.675196e-50 | -8.615850e-47 |
| Msa0203020 | Msa0680150 | 0.824552 | 7.587067e-54 | -8.615850e-47 |
| Msa0203020 | Msa0707220 | 0.803459 | 3.451075e-49 | -8.615850e-47 |
| Msa0203020 | Msa0876690 | 0.807127 | 5.882390e-50 | -8.615850e-47 |
| Msa0203020 | Msa0941960 | 0.807316 | 5.364144e-50 | -8.615850e-47 |
| Msa0203020 | Msa1228190 | 0.802979 | 4.338260e-49 | -8.615850e-47 |
| Msa0203020 | Msa1348400 | 0.800733 | 1.254917e-48 | -8.615850e-47 |
PPI
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| Msa0203020 | MtrunA17_Chr2g0300581 | 93.810 | 210 | 13 | 0 | 1 | 210 | 1 | 210 | 3.31e-144 | 400 |
| Msa0203020 | MtrunA17_Chr2g0300591 | 93.810 | 210 | 13 | 0 | 1 | 210 | 1 | 210 | 3.11e-143 | 397 |
| Msa0203020 | MtrunA17_Chr2g0300561 | 84.286 | 210 | 33 | 0 | 1 | 210 | 1 | 210 | 1.01e-127 | 358 |
| Msa0203020 | MtrunA17_Chr8g0343911 | 63.942 | 208 | 75 | 0 | 3 | 210 | 1 | 208 | 1.54e-91 | 267 |
| Msa0203020 | MtrunA17_Chr8g0343934 | 54.545 | 209 | 91 | 3 | 4 | 210 | 3 | 209 | 3.31e-78 | 233 |
| Msa0203020 | MtrunA17_Chr8g0343954 | 55.440 | 193 | 84 | 2 | 18 | 210 | 19 | 209 | 4.72e-76 | 228 |
| Msa0203020 | MtrunA17_Chr8g0343961 | 53.589 | 209 | 93 | 3 | 4 | 210 | 3 | 209 | 1.55e-75 | 226 |
| Msa0203020 | MtrunA17_Chr8g0343921 | 52.404 | 208 | 93 | 2 | 7 | 210 | 2 | 207 | 1.65e-72 | 218 |
| Msa0203020 | MtrunA17_Chr2g0301611 | 49.057 | 212 | 103 | 4 | 1 | 210 | 1 | 209 | 4.51e-69 | 210 |
| Msa0203020 | MtrunA17_Chr2g0302171 | 50.718 | 209 | 99 | 3 | 4 | 210 | 3 | 209 | 2.17e-68 | 208 |
| Msa0203020 | MtrunA17_Chr2g0313961 | 50.971 | 206 | 98 | 3 | 4 | 209 | 6 | 208 | 1.30e-67 | 206 |
| Msa0203020 | MtrunA17_Chr2g0297241 | 50.962 | 208 | 98 | 3 | 4 | 209 | 3 | 208 | 6.24e-67 | 204 |
| Msa0203020 | MtrunA17_Chr2g0297231 | 50.000 | 206 | 99 | 3 | 4 | 207 | 3 | 206 | 1.67e-66 | 203 |
| Msa0203020 | MtrunA17_Chr3g0084531 | 48.804 | 209 | 103 | 3 | 4 | 210 | 3 | 209 | 9.65e-66 | 201 |
| Msa0203020 | MtrunA17_Chr3g0084511 | 49.038 | 208 | 103 | 3 | 4 | 210 | 3 | 208 | 3.65e-65 | 200 |
| Msa0203020 | MtrunA17_Chr3g0084521 | 49.038 | 208 | 103 | 3 | 4 | 210 | 3 | 208 | 1.21e-64 | 198 |
| Msa0203020 | MtrunA17_Chr8g0344051 | 46.479 | 213 | 110 | 3 | 1 | 210 | 1 | 212 | 3.56e-62 | 192 |
| Msa0203020 | MtrunA17_Chr5g0411961 | 45.972 | 211 | 111 | 3 | 1 | 210 | 1 | 209 | 5.20e-60 | 187 |
| Msa0203020 | MtrunA17_Chr4g0003361 | 47.115 | 208 | 107 | 3 | 3 | 210 | 1 | 205 | 1.07e-58 | 183 |
| Msa0203020 | MtrunA17_Chr4g0012651 | 47.938 | 194 | 99 | 2 | 17 | 210 | 18 | 209 | 4.18e-58 | 182 |
| Msa0203020 | MtrunA17_Chr8g0344021 | 43.662 | 213 | 116 | 3 | 1 | 210 | 1 | 212 | 7.09e-58 | 181 |
| Msa0203020 | MtrunA17_Chr2g0302541 | 46.667 | 195 | 102 | 2 | 16 | 210 | 17 | 209 | 8.57e-58 | 181 |
| Msa0203020 | MtrunA17_Chr7g0217811 | 46.907 | 194 | 101 | 2 | 17 | 210 | 18 | 209 | 1.90e-57 | 180 |
| Msa0203020 | MtrunA17_Chr8g0344031 | 44.860 | 214 | 111 | 4 | 1 | 210 | 1 | 211 | 3.34e-56 | 177 |
| Msa0203020 | MtrunA17_Chr8g0344041 | 41.827 | 208 | 119 | 2 | 4 | 210 | 7 | 213 | 1.59e-55 | 176 |
| Msa0203020 | MtrunA17_Chr3g0115141 | 42.326 | 215 | 114 | 5 | 1 | 210 | 1 | 210 | 6.38e-55 | 174 |
| Msa0203020 | MtrunA17_Chr3g0084551 | 44.976 | 209 | 105 | 4 | 4 | 210 | 3 | 203 | 4.81e-52 | 167 |
| Msa0203020 | MtrunA17_Chr2g0286171 | 41.475 | 217 | 115 | 5 | 1 | 210 | 1 | 212 | 1.15e-51 | 166 |
| Msa0203020 | MtrunA17_Chr2g0292831 | 43.478 | 184 | 96 | 3 | 20 | 201 | 25 | 202 | 8.25e-44 | 145 |
| Msa0203020 | MtrunA17_Chr2g0292901 | 45.062 | 162 | 84 | 2 | 20 | 179 | 26 | 184 | 3.21e-42 | 141 |
| Msa0203020 | MtrunA17_Chr4g0007051 | 38.028 | 213 | 115 | 5 | 7 | 204 | 4 | 214 | 3.26e-39 | 134 |
| Msa0203020 | MtrunA17_Chr4g0007031 | 38.028 | 213 | 115 | 5 | 7 | 204 | 4 | 214 | 3.63e-39 | 134 |
| Msa0203020 | MtrunA17_Chr5g0419971 | 38.028 | 213 | 115 | 5 | 7 | 204 | 4 | 214 | 3.63e-39 | 134 |
| Msa0203020 | MtrunA17_Chr5g0419981 | 38.028 | 213 | 115 | 5 | 7 | 204 | 4 | 214 | 3.63e-39 | 134 |
| Msa0203020 | MtrunA17_Chr1g0156131 | 40.476 | 168 | 96 | 2 | 40 | 203 | 39 | 206 | 8.03e-39 | 132 |
| Msa0203020 | MtrunA17_Chr1g0189841 | 38.679 | 212 | 114 | 5 | 8 | 204 | 5 | 215 | 5.05e-38 | 131 |
| Msa0203020 | MtrunA17_Chr1g0178711 | 40.120 | 167 | 99 | 1 | 40 | 206 | 56 | 221 | 9.45e-38 | 130 |
| Msa0203020 | MtrunA17_Chr6g0449731 | 37.674 | 215 | 119 | 5 | 3 | 204 | 1 | 213 | 1.19e-37 | 130 |
| Msa0203020 | MtrunA17_Chr1g0210761 | 35.149 | 202 | 126 | 2 | 3 | 204 | 9 | 205 | 1.53e-37 | 129 |
| Msa0203020 | MtrunA17_Chr2g0285871 | 36.620 | 213 | 118 | 5 | 7 | 204 | 4 | 214 | 2.24e-37 | 129 |
| Msa0203020 | MtrunA17_Chr4g0007041 | 37.037 | 216 | 122 | 5 | 1 | 204 | 1 | 214 | 2.29e-37 | 129 |
| Msa0203020 | MtrunA17_Chr6g0449761 | 37.674 | 215 | 119 | 5 | 3 | 204 | 1 | 213 | 2.96e-37 | 130 |
| Msa0203020 | MtrunA17_Chr6g0449771 | 37.209 | 215 | 120 | 5 | 3 | 204 | 1 | 213 | 7.93e-37 | 128 |
| Msa0203020 | MtrunA17_Chr2g0292911 | 51.282 | 117 | 55 | 1 | 40 | 154 | 7 | 123 | 1.32e-36 | 125 |
| Msa0203020 | MtrunA17_Chr1g0193421 | 38.710 | 186 | 112 | 1 | 23 | 206 | 30 | 215 | 1.75e-36 | 127 |
| Msa0203020 | MtrunA17_Chr2g0292861 | 39.645 | 169 | 97 | 3 | 40 | 203 | 7 | 175 | 2.42e-36 | 125 |
| Msa0203020 | MtrunA17_Chr7g0271211 | 39.785 | 186 | 110 | 1 | 23 | 206 | 26 | 211 | 2.54e-36 | 126 |
| Msa0203020 | MtrunA17_Chr2g0292841 | 39.645 | 169 | 97 | 3 | 40 | 203 | 7 | 175 | 5.44e-36 | 124 |
| Msa0203020 | MtrunA17_Chr6g0449751 | 39.894 | 188 | 105 | 4 | 23 | 204 | 28 | 213 | 9.30e-36 | 125 |
| Msa0203020 | MtrunA17_Chr1g0193671 | 35.519 | 183 | 116 | 1 | 23 | 203 | 29 | 211 | 1.15e-32 | 117 |
| Msa0203020 | MtrunA17_Chr5g0414971 | 34.921 | 189 | 120 | 2 | 23 | 208 | 33 | 221 | 4.81e-32 | 115 |
| Msa0203020 | MtrunA17_Chr3g0130241 | 36.313 | 179 | 103 | 3 | 1 | 169 | 65 | 242 | 2.59e-30 | 113 |
| Msa0203020 | MtrunA17_Chr3g0084501 | 51.456 | 103 | 48 | 2 | 4 | 106 | 6 | 106 | 1.08e-29 | 106 |
| Msa0203020 | MtrunA17_Chr8g0335851 | 36.723 | 177 | 99 | 5 | 4 | 169 | 5 | 179 | 1.88e-29 | 108 |
| Msa0203020 | MtrunA17_Chr6g0449791 | 38.509 | 161 | 92 | 3 | 14 | 169 | 19 | 177 | 1.88e-29 | 108 |
| Msa0203020 | MtrunA17_Chr6g0449781 | 35.829 | 187 | 113 | 4 | 23 | 204 | 28 | 212 | 2.44e-29 | 108 |
| Msa0203020 | MtrunA17_Chr2g0321591 | 32.367 | 207 | 124 | 5 | 2 | 204 | 8 | 202 | 2.03e-25 | 98.2 |
| Msa0203020 | MtrunA17_Chr2g0321581 | 32.367 | 207 | 124 | 5 | 2 | 204 | 8 | 202 | 2.40e-25 | 98.2 |
| Msa0203020 | MtrunA17_Chr2g0321601 | 31.429 | 210 | 122 | 5 | 2 | 204 | 7 | 201 | 2.75e-24 | 95.1 |
| Msa0203020 | MtrunA17_Chr4g0026191 | 28.972 | 214 | 126 | 7 | 1 | 204 | 6 | 203 | 9.22e-19 | 80.9 |
| Msa0203020 | MtrunA17_Chr4g0026201 | 33.077 | 130 | 86 | 1 | 76 | 204 | 74 | 203 | 1.14e-18 | 80.5 |
| Msa0203020 | MtrunA17_Chr2g0327651 | 29.703 | 101 | 69 | 2 | 43 | 142 | 6 | 105 | 1.20e-11 | 59.7 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| Msa0203020 | AT5G20630.1 | 64.762 | 210 | 73 | 1 | 1 | 209 | 1 | 210 | 1.51e-95 | 277 |
| Msa0203020 | AT1G72610.1 | 62.019 | 208 | 79 | 0 | 3 | 210 | 1 | 208 | 2.30e-85 | 251 |
| Msa0203020 | AT3G04200.1 | 43.979 | 191 | 101 | 3 | 23 | 207 | 33 | 223 | 7.54e-43 | 144 |
| Msa0203020 | AT5G39160.3 | 42.105 | 190 | 102 | 3 | 23 | 207 | 30 | 216 | 4.51e-41 | 139 |
| Msa0203020 | AT5G39190.1 | 42.408 | 191 | 104 | 3 | 23 | 207 | 30 | 220 | 2.77e-40 | 137 |
| Msa0203020 | AT5G39160.1 | 42.408 | 191 | 104 | 3 | 23 | 207 | 30 | 220 | 3.40e-40 | 137 |
| Msa0203020 | AT5G39130.1 | 41.885 | 191 | 105 | 3 | 23 | 207 | 30 | 220 | 1.08e-39 | 135 |
| Msa0203020 | AT1G18980.1 | 39.901 | 203 | 117 | 3 | 3 | 203 | 16 | 215 | 1.32e-39 | 135 |
| Msa0203020 | AT5G39110.1 | 41.489 | 188 | 103 | 3 | 23 | 203 | 30 | 217 | 1.77e-39 | 135 |
| Msa0203020 | AT1G18970.1 | 39.130 | 207 | 120 | 3 | 1 | 203 | 11 | 215 | 2.75e-39 | 134 |
| Msa0203020 | AT5G39120.1 | 38.208 | 212 | 122 | 4 | 1 | 203 | 5 | 216 | 4.59e-39 | 134 |
| Msa0203020 | AT5G38960.1 | 41.327 | 196 | 100 | 6 | 23 | 207 | 32 | 223 | 7.78e-39 | 133 |
| Msa0203020 | AT3G05950.1 | 42.553 | 188 | 100 | 4 | 23 | 203 | 32 | 218 | 1.10e-38 | 133 |
| Msa0203020 | AT3G62020.1 | 36.098 | 205 | 121 | 3 | 9 | 203 | 8 | 212 | 5.21e-38 | 131 |
| Msa0203020 | AT5G61750.1 | 40.223 | 179 | 91 | 3 | 1 | 169 | 1 | 173 | 5.86e-38 | 131 |
| Msa0203020 | AT5G39150.1 | 37.383 | 214 | 123 | 4 | 1 | 203 | 3 | 216 | 1.39e-37 | 130 |
| Msa0203020 | AT5G39180.1 | 37.383 | 214 | 123 | 4 | 1 | 203 | 3 | 216 | 2.15e-37 | 130 |
| Msa0203020 | AT1G02335.1 | 34.146 | 205 | 133 | 1 | 5 | 207 | 12 | 216 | 3.84e-37 | 129 |
| Msa0203020 | AT3G04150.1 | 40.000 | 195 | 109 | 4 | 23 | 210 | 32 | 225 | 5.05e-37 | 129 |
| Msa0203020 | AT3G04150.2 | 40.223 | 179 | 102 | 2 | 37 | 210 | 60 | 238 | 2.58e-36 | 127 |
| Msa0203020 | AT5G38940.1 | 45.033 | 151 | 79 | 2 | 23 | 169 | 32 | 182 | 3.33e-36 | 127 |
| Msa0203020 | AT3G04190.1 | 43.293 | 164 | 87 | 3 | 23 | 181 | 32 | 194 | 4.82e-36 | 126 |
| Msa0203020 | AT5G38940.2 | 45.033 | 151 | 79 | 2 | 23 | 169 | 56 | 206 | 5.57e-36 | 127 |
| Msa0203020 | AT3G10080.1 | 37.566 | 189 | 112 | 2 | 21 | 204 | 30 | 217 | 1.68e-35 | 125 |
| Msa0203020 | AT5G38930.1 | 45.033 | 151 | 79 | 2 | 23 | 169 | 32 | 182 | 2.04e-35 | 125 |
| Msa0203020 | AT3G04180.1 | 44.242 | 165 | 84 | 5 | 23 | 181 | 32 | 194 | 3.09e-35 | 124 |
| Msa0203020 | AT5G26700.1 | 35.149 | 202 | 125 | 3 | 3 | 203 | 10 | 206 | 3.64e-35 | 124 |
| Msa0203020 | AT1G09560.1 | 42.373 | 177 | 100 | 1 | 8 | 184 | 17 | 191 | 6.20e-35 | 123 |
| Msa0203020 | AT3G10080.2 | 37.566 | 189 | 112 | 2 | 21 | 204 | 101 | 288 | 6.98e-35 | 125 |
| Msa0203020 | AT3G05930.1 | 37.981 | 208 | 121 | 3 | 4 | 203 | 5 | 212 | 1.42e-34 | 122 |
| Msa0203020 | AT3G62020.2 | 37.059 | 170 | 104 | 2 | 37 | 203 | 14 | 183 | 1.89e-34 | 121 |
| Msa0203020 | AT5G38910.1 | 45.122 | 164 | 82 | 3 | 14 | 169 | 17 | 180 | 8.33e-34 | 120 |
| Msa0203020 | AT5G39190.2 | 41.718 | 163 | 90 | 2 | 50 | 207 | 36 | 198 | 1.18e-33 | 119 |
| Msa0203020 | AT5G39160.2 | 41.718 | 163 | 90 | 2 | 50 | 207 | 36 | 198 | 1.28e-33 | 119 |
| Msa0203020 | AT3G04170.1 | 39.791 | 191 | 104 | 6 | 23 | 206 | 32 | 218 | 1.30e-33 | 120 |
| Msa0203020 | AT4G14630.1 | 37.766 | 188 | 110 | 3 | 23 | 203 | 32 | 219 | 1.19e-31 | 115 |
| Msa0203020 | AT1G10460.1 | 37.079 | 178 | 104 | 4 | 37 | 208 | 42 | 217 | 6.66e-31 | 113 |
| Msa0203020 | AT1G74820.1 | 32.850 | 207 | 133 | 3 | 1 | 203 | 18 | 222 | 3.09e-30 | 111 |
| Msa0203020 | AT5G38910.2 | 34.568 | 162 | 65 | 3 | 14 | 169 | 17 | 143 | 1.98e-15 | 71.6 |
Find 46 sgRNAs with CRISPR-Local
Find 49 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| TAGAACGACCAACATTAATT+TGG | 0.178448 | 2_1:-26215169 | None:intergenic |
| TAATCCTCGTTTCCAGATAT+TGG | 0.294879 | 2_1:+26215223 | Msa0203020:CDS |
| GAAGTGTTCGTTGTTATTAA+AGG | 0.300904 | 2_1:+26215047 | Msa0203020:CDS |
| ACAAAGCGTAATCCAATATC+TGG | 0.309210 | 2_1:-26215235 | None:intergenic |
| TCATTCGCGGTTACCTTTGA+TGG | 0.323535 | 2_1:-26214849 | None:intergenic |
| AAGAAGCTTAAGCGTATTCT+TGG | 0.334205 | 2_1:+26215320 | Msa0203020:CDS |
| GAATATCCAGCTGGGCCATT+TGG | 0.360878 | 2_1:-26214816 | None:intergenic |
| GCATTTGACGCACAATTTCC+CGG | 0.369797 | 2_1:+26214939 | Msa0203020:CDS |
| GCACAATTTCCCGGTGTAAA+CGG | 0.386040 | 2_1:+26214948 | Msa0203020:CDS |
| TTTCCCGGTGTAAACGGTCT+TGG | 0.446321 | 2_1:+26214954 | Msa0203020:CDS |
| AATGCACCAAATGGCCCAGC+TGG | 0.454211 | 2_1:+26214810 | Msa0203020:CDS |
| TCACACACACCCCGGTGCTT+CGG | 0.471434 | 2_1:+26215025 | Msa0203020:CDS |
| GGCTAAATCTAAACGCGCAA+TGG | 0.477631 | 2_1:-26214982 | None:intergenic |
| TACCACGGCCTAGCTGCAGC+TGG | 0.478895 | 2_1:+26214879 | Msa0203020:CDS |
| ATTAAAGGAACAATTAGTGC+TGG | 0.479341 | 2_1:+26215062 | Msa0203020:CDS |
| AACGAACACTTCCGAAGCAC+CGG | 0.491763 | 2_1:-26215036 | None:intergenic |
| AGCGGCTTTGATAATGTTTG+TGG | 0.500309 | 2_1:-26214907 | None:intergenic |
| AAGCGTATTCTTGGAGGCAG+TGG | 0.501763 | 2_1:+26215329 | Msa0203020:CDS |
| AAGCTTAAGCGTATTCTTGG+AGG | 0.506351 | 2_1:+26215323 | Msa0203020:CDS |
| TGGTGTTTCCAGCTGCAGCT+AGG | 0.511440 | 2_1:-26214887 | None:intergenic |
| TTGCATTTCCAAATTAATGT+TGG | 0.515849 | 2_1:+26215161 | Msa0203020:CDS |
| TTCCAGCTGCAGCTAGGCCG+TGG | 0.519147 | 2_1:-26214881 | None:intergenic |
| GGTTTGCAAGAATATCCAGC+TGG | 0.520856 | 2_1:-26214825 | None:intergenic |
| GTTGCAGTAATTAACTCCGT+TGG | 0.523155 | 2_1:-26215272 | None:intergenic |
| GCAGATTACAATGCACCAAA+TGG | 0.532463 | 2_1:+26214801 | Msa0203020:CDS |
| ACGAACACTTCCGAAGCACC+GGG | 0.547731 | 2_1:-26215035 | None:intergenic |
| TTCTTGCAAACCACCATCAA+AGG | 0.552457 | 2_1:+26214836 | Msa0203020:CDS |
| AAGGTAAACAACATTGTCAG+AGG | 0.569703 | 2_1:-26215093 | None:intergenic |
| TTCGCGGTTACCTTTGATGG+TGG | 0.572308 | 2_1:-26214846 | None:intergenic |
| CGTTTAGATTTAGCCGTCGG+TGG | 0.577924 | 2_1:+26214990 | Msa0203020:CDS |
| ATTCCAAGACCGTTTACACC+GGG | 0.600502 | 2_1:-26214957 | None:intergenic |
| TGGAAATGCAACAATCCTTG+TGG | 0.601071 | 2_1:-26215149 | None:intergenic |
| GGGAAATTGTGCGTCAAATG+CGG | 0.602752 | 2_1:-26214937 | None:intergenic |
| TACCTTAAAACACTTCACAA+AGG | 0.608858 | 2_1:+26215110 | Msa0203020:CDS |
| TGCTACGCAGAAATCTACTA+CGG | 0.612239 | 2_1:-26214781 | None:intergenic |
| GCGCGTTTAGATTTAGCCGT+CGG | 0.614388 | 2_1:+26214987 | Msa0203020:CDS |
| GATTCCAAGACCGTTTACAC+CGG | 0.616073 | 2_1:-26214958 | None:intergenic |
| GCACCGGGGTGTGTGTGAAG+TGG | 0.621649 | 2_1:-26215020 | None:intergenic |
| GTGGTAGACGAAGTCATTCG+CGG | 0.625632 | 2_1:-26214862 | None:intergenic |
| GTTTGCAAGAATATCCAGCT+GGG | 0.638814 | 2_1:-26214824 | None:intergenic |
| AAGTGGAACAACACCACCGA+CGG | 0.642171 | 2_1:-26215003 | None:intergenic |
| GTCAAATGCGGTGCTAACAG+CGG | 0.644054 | 2_1:-26214925 | None:intergenic |
| GTTCCACTTCACACACACCC+CGG | 0.667019 | 2_1:+26215017 | Msa0203020:CDS |
| TAATCCAATATCTGGAAACG+AGG | 0.691373 | 2_1:-26215227 | None:intergenic |
| GCGAATGACTTCGTCTACCA+CGG | 0.705558 | 2_1:+26214864 | Msa0203020:CDS |
| CGAACACTTCCGAAGCACCG+GGG | 0.778153 | 2_1:-26215034 | None:intergenic |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| ! | TTGCATTTCCAAATTAATGT+TGG | + | chr2_1:26215161-26215180 | Msa0203020:CDS | 25.0% |
| ! | GAAGTGTTCGTTGTTATTAA+AGG | + | chr2_1:26215047-26215066 | Msa0203020:CDS | 30.0% |
| ATTAAAGGAACAATTAGTGC+TGG | + | chr2_1:26215062-26215081 | Msa0203020:CDS | 30.0% | |
| TACCTTAAAACACTTCACAA+AGG | + | chr2_1:26215110-26215129 | Msa0203020:CDS | 30.0% | |
| ! | GATGTTATGATTTTTCCACA+AGG | + | chr2_1:26215134-26215153 | Msa0203020:CDS | 30.0% |
| TAGAACGACCAACATTAATT+TGG | - | chr2_1:26215172-26215191 | None:intergenic | 30.0% | |
| AAGGTAAACAACATTGTCAG+AGG | - | chr2_1:26215096-26215115 | None:intergenic | 35.0% | |
| !! | CACCTTTGTGAAGTGTTTTA+AGG | - | chr2_1:26215115-26215134 | None:intergenic | 35.0% |
| TAATCCTCGTTTCCAGATAT+TGG | + | chr2_1:26215223-26215242 | Msa0203020:CDS | 35.0% | |
| TAATCCAATATCTGGAAACG+AGG | - | chr2_1:26215230-26215249 | None:intergenic | 35.0% | |
| ACAAAGCGTAATCCAATATC+TGG | - | chr2_1:26215238-26215257 | None:intergenic | 35.0% | |
| ! | GTTTGAAAGCGATTTTCCAA+CGG | + | chr2_1:26215256-26215275 | Msa0203020:CDS | 35.0% |
| AAGAAGCTTAAGCGTATTCT+TGG | + | chr2_1:26215320-26215339 | Msa0203020:CDS | 35.0% | |
| TGCTACGCAGAAATCTACTA+CGG | - | chr2_1:26214784-26214803 | None:intergenic | 40.0% | |
| GCAGATTACAATGCACCAAA+TGG | + | chr2_1:26214801-26214820 | Msa0203020:CDS | 40.0% | |
| GTTTGCAAGAATATCCAGCT+GGG | - | chr2_1:26214827-26214846 | None:intergenic | 40.0% | |
| TTCTTGCAAACCACCATCAA+AGG | + | chr2_1:26214836-26214855 | Msa0203020:CDS | 40.0% | |
| !! | AGCGGCTTTGATAATGTTTG+TGG | - | chr2_1:26214910-26214929 | None:intergenic | 40.0% |
| TGGAAATGCAACAATCCTTG+TGG | - | chr2_1:26215152-26215171 | None:intergenic | 40.0% | |
| GTTGCAGTAATTAACTCCGT+TGG | - | chr2_1:26215275-26215294 | None:intergenic | 40.0% | |
| AAGCTTAAGCGTATTCTTGG+AGG | + | chr2_1:26215323-26215342 | Msa0203020:CDS | 40.0% | |
| GGTTTGCAAGAATATCCAGC+TGG | - | chr2_1:26214828-26214847 | None:intergenic | 45.0% | |
| TCATTCGCGGTTACCTTTGA+TGG | - | chr2_1:26214852-26214871 | None:intergenic | 45.0% | |
| GGGAAATTGTGCGTCAAATG+CGG | - | chr2_1:26214940-26214959 | None:intergenic | 45.0% | |
| GCATTTGACGCACAATTTCC+CGG | + | chr2_1:26214939-26214958 | Msa0203020:CDS | 45.0% | |
| GCACAATTTCCCGGTGTAAA+CGG | + | chr2_1:26214948-26214967 | Msa0203020:CDS | 45.0% | |
| ATTCCAAGACCGTTTACACC+GGG | - | chr2_1:26214960-26214979 | None:intergenic | 45.0% | |
| GATTCCAAGACCGTTTACAC+CGG | - | chr2_1:26214961-26214980 | None:intergenic | 45.0% | |
| GGCTAAATCTAAACGCGCAA+TGG | - | chr2_1:26214985-26215004 | None:intergenic | 45.0% | |
| GAATATCCAGCTGGGCCATT+TGG | - | chr2_1:26214819-26214838 | None:intergenic | 50.0% | |
| TTCGCGGTTACCTTTGATGG+TGG | - | chr2_1:26214849-26214868 | None:intergenic | 50.0% | |
| GTGGTAGACGAAGTCATTCG+CGG | - | chr2_1:26214865-26214884 | None:intergenic | 50.0% | |
| GCGAATGACTTCGTCTACCA+CGG | + | chr2_1:26214864-26214883 | Msa0203020:CDS | 50.0% | |
| ! | GTCAAATGCGGTGCTAACAG+CGG | - | chr2_1:26214928-26214947 | None:intergenic | 50.0% |
| TTTCCCGGTGTAAACGGTCT+TGG | + | chr2_1:26214954-26214973 | Msa0203020:CDS | 50.0% | |
| GCGCGTTTAGATTTAGCCGT+CGG | + | chr2_1:26214987-26215006 | Msa0203020:CDS | 50.0% | |
| CGTTTAGATTTAGCCGTCGG+TGG | + | chr2_1:26214990-26215009 | Msa0203020:CDS | 50.0% | |
| AAGTGGAACAACACCACCGA+CGG | - | chr2_1:26215006-26215025 | None:intergenic | 50.0% | |
| AACGAACACTTCCGAAGCAC+CGG | - | chr2_1:26215039-26215058 | None:intergenic | 50.0% | |
| AAGCGTATTCTTGGAGGCAG+TGG | + | chr2_1:26215329-26215348 | Msa0203020:CDS | 50.0% | |
| AATGCACCAAATGGCCCAGC+TGG | + | chr2_1:26214810-26214829 | Msa0203020:CDS | 55.0% | |
| TGGTGTTTCCAGCTGCAGCT+AGG | - | chr2_1:26214890-26214909 | None:intergenic | 55.0% | |
| GTTCCACTTCACACACACCC+CGG | + | chr2_1:26215017-26215036 | Msa0203020:CDS | 55.0% | |
| ! | ACGAACACTTCCGAAGCACC+GGG | - | chr2_1:26215038-26215057 | None:intergenic | 55.0% |
| TCACACACACCCCGGTGCTT+CGG | + | chr2_1:26215025-26215044 | Msa0203020:CDS | 60.0% | |
| !! | CGAACACTTCCGAAGCACCG+GGG | - | chr2_1:26215037-26215056 | None:intergenic | 60.0% |
| TACCACGGCCTAGCTGCAGC+TGG | + | chr2_1:26214879-26214898 | Msa0203020:CDS | 65.0% | |
| TTCCAGCTGCAGCTAGGCCG+TGG | - | chr2_1:26214884-26214903 | None:intergenic | 65.0% | |
| GCACCGGGGTGTGTGTGAAG+TGG | - | chr2_1:26215023-26215042 | None:intergenic | 65.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr2_1 | gene | 26214723 | 26215355 | 26214723 | ID=Msa0203020;Name=Msa0203020 |
| chr2_1 | mRNA | 26214723 | 26215355 | 26214723 | ID=Msa0203020-mRNA-1;Parent=Msa0203020;Name=Msa0203020-mRNA-1;_AED=0.36;_eAED=0.36;_QI=0|-1|0|1|-1|1|1|0|210 |
| chr2_1 | exon | 26214723 | 26215355 | 26214723 | ID=Msa0203020-mRNA-1:exon:9866;Parent=Msa0203020-mRNA-1 |
| chr2_1 | CDS | 26214723 | 26215355 | 26214723 | ID=Msa0203020-mRNA-1:cds;Parent=Msa0203020-mRNA-1 |
| Gene Sequence |
| Protein sequence |
