AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa0246910

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0246910 MtrunA17_Chr4g0031901 97.445 587 13 1 1 587 1 585 0.0 1170
Msa0246910 MtrunA17_Chr7g0238351 73.540 582 142 6 14 587 4 581 0.0 890
Msa0246910 MtrunA17_Chr7g0237491 72.355 586 147 6 11 587 1 580 0.0 874
Msa0246910 MtrunA17_Chr7g0237441 70.578 588 158 5 7 587 1 580 0.0 868
Msa0246910 MtrunA17_Chr7g0238941 70.486 576 157 5 16 587 12 578 0.0 863
Msa0246910 MtrunA17_Chr7g0238981 66.966 557 175 4 37 587 40 593 0.0 785
Msa0246910 MtrunA17_Chr5g0429511 54.827 549 232 7 41 587 24 558 0.0 625
Msa0246910 MtrunA17_Chr3g0104061 54.562 548 234 5 41 587 23 556 0.0 624
Msa0246910 MtrunA17_Chr4g0005921 54.862 545 234 5 44 587 24 557 0.0 622
Msa0246910 MtrunA17_Chr5g0404341 53.370 549 241 6 41 587 31 566 0.0 619
Msa0246910 MtrunA17_Chr5g0404361 51.736 576 260 8 14 587 52 611 0.0 613
Msa0246910 MtrunA17_Chr5g0436461 51.404 570 256 6 19 587 5 554 0.0 599
Msa0246910 MtrunA17_Chr5g0437681 51.127 577 261 10 20 587 3 567 0.0 592
Msa0246910 MtrunA17_Chr3g0114211 50.862 580 251 11 20 587 13 570 0.0 582
Msa0246910 MtrunA17_Chr5g0431581 49.656 582 263 12 13 587 2 560 0.0 546
Msa0246910 MtrunA17_Chr8g0346581 46.998 583 278 11 21 587 4 571 3.92e-180 521
Msa0246910 MtrunA17_Chr2g0278811 43.403 576 288 6 44 587 38 607 7.42e-174 506
Msa0246910 MtrunA17_Chr7g0240991 44.621 567 297 8 31 587 12 571 1.29e-173 504
Msa0246910 MtrunA17_Chr7g0240971 47.049 576 281 11 25 587 6 570 9.20e-170 494
Msa0246910 MtrunA17_Chr4g0008521 45.144 556 282 7 45 587 27 572 6.21e-168 489
Msa0246910 MtrunA17_Chr3g0133861 41.888 339 182 9 245 577 1 330 1.39e-75 244
Msa0246910 MtrunA17_Chr3g0133871 47.706 218 113 1 13 230 2 218 1.30e-66 216
Msa0246910 MtrunA17_Chr8g0382691 30.526 570 322 20 44 577 34 565 6.35e-65 223
Msa0246910 MtrunA17_Chr3g0118321 31.218 583 314 21 45 587 27 562 1.16e-62 216
Msa0246910 MtrunA17_Chr3g0118331 31.250 576 313 24 44 577 25 559 6.71e-61 211
Msa0246910 MtrunA17_Chr5g0436431 46.341 205 101 3 342 545 32 228 5.87e-58 194
Msa0246910 MtrunA17_Chr4g0056511 27.381 588 363 15 19 587 7 549 4.76e-48 177
Msa0246910 MtrunA17_Chr3g0079321 26.401 553 348 14 19 552 4 516 1.01e-45 170
Msa0246910 MtrunA17_Chr1g0157341 24.908 546 366 14 44 587 29 532 1.77e-35 140
Msa0246910 MtrunA17_Chr7g0234061 26.128 532 322 18 45 552 30 514 4.70e-35 139
Msa0246910 MtrunA17_Chr3g0143671 23.498 566 349 14 44 587 30 533 9.49e-33 132
Msa0246910 MtrunA17_Chr4g0024861 24.855 519 320 14 44 547 27 490 1.22e-31 129
Msa0246910 MtrunA17_Chr4g0038671 23.878 557 357 15 44 587 30 532 1.34e-30 125
Msa0246910 MtrunA17_Chr2g0322441 24.478 527 312 14 44 547 30 493 6.27e-29 120
Msa0246910 MtrunA17_Chr3g0121171 24.955 561 359 17 45 582 27 548 1.71e-28 121
Msa0246910 MtrunA17_Chr3g0121171 24.468 564 358 20 45 582 1177 1698 2.87e-28 120
Msa0246910 MtrunA17_Chr3g0121171 24.339 567 355 18 45 582 602 1123 2.02e-27 118
Msa0246910 MtrunA17_Chr3g0121171 24.414 512 313 18 100 582 1798 2264 3.52e-22 102
Msa0246910 MtrunA17_Chr5g0402831 25.641 507 327 15 44 547 31 490 2.93e-28 119
Msa0246910 MtrunA17_Chr8g0374261 24.376 521 346 16 33 547 13 491 3.29e-28 119
Msa0246910 MtrunA17_Chr3g0121151 24.144 555 364 16 45 587 67 576 4.40e-28 119
Msa0246910 MtrunA17_Chr3g0118351 31.534 352 187 14 255 577 5 331 4.64e-28 115
Msa0246910 MtrunA17_Chr3g0121161 24.605 569 351 19 45 582 67 588 5.48e-28 118
Msa0246910 MtrunA17_Chr4g0043261 24.717 530 340 16 45 563 26 507 3.07e-27 115
Msa0246910 MtrunA17_Chr8g0374271 24.085 519 323 16 44 547 31 493 8.32e-27 114
Msa0246910 MtrunA17_Chr4g0075071 24.706 510 332 16 44 547 31 494 2.75e-25 110
Msa0246910 MtrunA17_Chr4g0043251 25.000 532 356 17 19 547 6 497 2.24e-23 104
Msa0246910 MtrunA17_Chr3g0118341 32.961 179 109 7 40 212 22 195 3.46e-22 95.1
Msa0246910 MtrunA17_Chr1g0197301 27.446 368 212 14 233 587 4 329 6.88e-20 91.3
Msa0246910 MtrunA17_Chr4g0024641 23.830 470 283 18 96 547 1 413 7.62e-17 84.7
Msa0246910 MtrunA17_Chr5g0436411 44.444 81 39 1 19 99 5 79 1.01e-15 72.4
Msa0246910 MtrunA17_Chr3g0118931 39.080 87 46 2 497 578 10 94 3.81e-12 63.2
Msa0246910 MtrunA17_Chr1g0197311 27.619 210 115 6 58 234 38 243 5.17e-11 63.2
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0246910 AT2G29130.1 70.139 576 158 6 15 587 9 573 0.0 859
Msa0246910 AT5G60020.1 69.430 579 156 6 20 587 9 577 0.0 850
Msa0246910 AT5G60020.3 71.664 547 142 5 52 587 1 545 0.0 833
Msa0246910 AT5G60020.2 69.088 537 145 6 20 545 9 535 0.0 778
Msa0246910 AT2G38080.1 54.306 569 242 6 19 587 8 558 0.0 634
Msa0246910 AT5G58910.2 52.028 567 252 8 25 587 17 567 0.0 608
Msa0246910 AT5G03260.1 52.007 573 253 9 18 587 4 557 0.0 608
Msa0246910 AT5G58910.3 52.028 567 251 9 25 587 17 566 0.0 604
Msa0246910 AT2G40370.1 51.178 594 257 11 11 587 3 580 0.0 592
Msa0246910 AT5G05390.1 53.276 580 242 11 16 587 7 565 0.0 591
Msa0246910 AT5G01190.1 52.000 575 256 8 15 587 2 558 0.0 588
Msa0246910 AT1G18140.1 50.622 563 257 10 40 587 25 581 0.0 578
Msa0246910 AT2G30210.1 50.172 580 268 9 16 587 4 570 0.0 571
Msa0246910 AT5G58910.1 51.296 540 242 7 52 587 1 523 0.0 570
Msa0246910 AT5G01190.2 51.130 575 247 9 15 587 2 544 0.0 565
Msa0246910 AT5G07130.1 50.090 553 256 11 46 587 26 569 0.0 529
Msa0246910 AT2G46570.1 44.905 579 290 11 20 587 9 569 2.37e-180 521
Msa0246910 AT5G09360.1 44.406 572 292 12 22 587 18 569 4.06e-172 500
Msa0246910 AT3G09220.1 46.435 547 274 8 32 570 15 550 3.60e-171 498
Msa0246910 AT5G48100.1 45.505 545 277 11 42 577 20 553 3.09e-163 478
Msa0246910 AT5G07130.2 50.101 493 226 11 106 587 1 484 4.27e-158 461
Msa0246910 AT5G07130.3 50.101 493 226 11 106 587 1 484 4.27e-158 461
Msa0246910 AT5G01050.1 42.606 568 299 10 32 587 16 568 1.50e-154 456
Msa0246910 AT2G46570.2 44.711 501 249 10 98 587 1 484 5.67e-152 446
Msa0246910 AT5G01040.1 42.226 566 302 10 32 587 16 566 1.52e-151 448
Msa0246910 AT3G09220.2 47.619 462 224 7 117 570 2 453 1.85e-142 421
Msa0246910 AT5G21105.1 30.653 597 346 19 17 577 9 573 1.09e-68 233
Msa0246910 AT4G39830.1 30.619 565 317 18 44 570 36 563 1.14e-68 233
Msa0246910 AT5G21100.1 30.515 544 302 17 59 566 39 542 5.83e-67 228
Msa0246910 AT5G21105.3 30.851 564 321 21 17 545 9 538 6.48e-60 208
Msa0246910 AT4G39830.2 29.821 503 279 17 105 570 3 468 1.29e-49 179
Msa0246910 AT4G39830.3 29.920 498 275 17 110 570 6 466 3.47e-49 178
Msa0246910 AT4G12420.1 26.996 526 325 13 46 552 27 512 2.09e-46 172
Msa0246910 AT4G12420.2 26.996 526 325 13 46 552 27 512 2.09e-46 172
Msa0246910 AT4G39830.4 28.884 502 272 16 105 570 3 455 7.48e-45 166
Msa0246910 AT1G75790.1 27.509 578 369 19 20 587 4 541 4.32e-44 165
Msa0246910 AT5G51480.1 27.725 523 315 15 46 547 30 510 5.00e-44 166
Msa0246910 AT4G25240.2 26.644 593 359 17 15 580 1 544 1.48e-43 164
Msa0246910 AT4G25240.1 26.644 593 359 17 15 580 1 544 1.48e-43 164
Msa0246910 AT5G21105.2 32.033 359 208 10 228 577 51 382 1.38e-38 147
Msa0246910 AT1G21860.1 25.000 540 345 14 23 553 8 496 4.06e-37 145
Msa0246910 AT5G48450.2 26.731 520 340 17 46 561 31 513 1.47e-36 144
Msa0246910 AT1G21850.1 24.205 566 346 17 1 553 1 496 2.25e-36 143
Msa0246910 AT5G48450.1 26.731 520 340 17 46 561 31 513 2.33e-36 144
Msa0246910 AT1G76160.1 24.660 588 369 17 13 587 4 530 1.29e-32 132
Msa0246910 AT4G22010.1 25.312 561 346 20 44 587 26 530 4.36e-31 127
Msa0246910 AT1G41830.1 23.717 565 387 14 24 587 10 531 8.13e-31 127
Msa0246910 AT5G66920.1 23.883 515 336 14 44 551 37 502 1.33e-30 126
Msa0246910 AT3G13390.1 25.540 509 335 14 45 547 29 499 5.69e-27 115
Msa0246910 AT4G28090.1 22.587 549 380 13 43 587 27 534 8.52e-27 115
Msa0246910 AT4G37160.1 23.935 493 333 13 62 553 49 500 9.75e-27 114
Msa0246910 AT3G13400.2 25.000 504 343 14 45 547 28 497 1.95e-25 110
Msa0246910 AT3G13400.1 25.000 504 343 14 45 547 28 497 1.95e-25 110
Msa0246910 AT1G55570.1 24.852 507 341 15 45 547 30 500 2.11e-25 110
Msa0246910 AT4G38420.1 21.526 511 365 13 44 553 30 505 8.60e-25 108
Msa0246910 AT2G23630.1 25.462 487 320 14 62 547 48 492 7.26e-24 106
Msa0246910 AT2G23630.2 25.462 487 320 14 62 547 53 497 7.80e-24 105
Msa0246910 AT1G55560.1 29.455 275 183 7 62 335 44 308 1.75e-23 105
Msa0246910 AT4G38420.3 21.355 487 347 13 44 529 30 481 1.58e-20 95.5
Msa0246910 AT4G38420.2 26.087 276 193 6 44 318 30 295 1.70e-20 95.1
Msa0246910 AT4G37160.2 27.016 248 169 4 62 308 49 285 4.48e-20 93.6

Find 172 sgRNAs with CRISPR-Local

Find 248 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
TCCATTTCCATGTAAATTTA+AGG 0.077214 2_2:+28703680 Msa0246910:three_prime_UTR
GGCACATTTGCAGGGTATTT+TGG 0.173773 2_2:-28702474 None:intergenic
TCTGATGCCTACACCATTAA+TGG 0.176037 2_2:+28701425 Msa0246910:CDS
ATTGCAGGATACAAGCATTT+TGG 0.187461 2_2:+28702812 Msa0246910:CDS
TTTGAATTTGAACTATTATT+TGG 0.212461 2_2:-28702576 None:intergenic
TGCACCTTAGTTGGGGATTA+AGG 0.227331 2_2:+28703501 Msa0246910:CDS
CTTCTCCAACAACATTTCTT+TGG 0.228697 2_2:+28702648 Msa0246910:CDS
CATGTAAATTTAAGGATTTA+TGG 0.246154 2_2:+28703688 Msa0246910:three_prime_UTR
CATTTCTTTGGAACTAATAA+TGG 0.256030 2_2:+28702660 Msa0246910:CDS
AACCTCCCCTGGATTTGTTT+TGG 0.259173 2_2:+28700247 Msa0246910:CDS
TTTGTTGTTGGTCAAGGTTT+TGG 0.271917 2_2:+28702876 Msa0246910:CDS
AATATCATTGTACTTACTTA+AGG 0.293923 2_2:+28703867 Msa0246910:three_prime_UTR
GTTTCTTTAGTAAGGAAAAT+TGG 0.294923 2_2:-28703623 None:intergenic
TAACTCTGACCTGTTTGAAT+TGG 0.297459 2_2:-28700973 None:intergenic
CTTTGATGTGCACCTTAGTT+GGG 0.307238 2_2:+28703493 Msa0246910:CDS
AATACTGTTGCTGTGCCTTC+TGG 0.309543 2_2:+28702957 Msa0246910:CDS
AATGTGGCATTTGGGTAATT+TGG 0.312994 2_2:-28702258 None:intergenic
ACGAGAAGGGGACAGATTAT+TGG 0.313664 2_2:+28700548 Msa0246910:CDS
CAATGCAGGCTGATATTGTT+TGG 0.314456 2_2:-28700591 None:intergenic
TGCTGTGCCTTCTGGTGGTT+GGG 0.319801 2_2:+28702965 Msa0246910:CDS
TACACCATTAATGGGTTTCC+TGG 0.319870 2_2:+28701434 Msa0246910:CDS
TTGGTGTAGTAATCAACATT+TGG 0.322522 2_2:-28703591 None:intergenic
TGGTGTAGTAATCAACATTT+GGG 0.326399 2_2:-28703590 None:intergenic
AGTCATCCTTTGCATCTTCA+TGG 0.334535 2_2:+28702843 Msa0246910:CDS
AAGGAAGTCCCCATTCTCTT+TGG 0.338186 2_2:+28701135 Msa0246910:CDS
ACCTGAACTCTAGTGTTATA+TGG 0.345887 2_2:-28702786 None:intergenic
GCTTGTGCAATTACAGCTTC+AGG 0.346802 2_2:-28701374 None:intergenic
TCCTTAAATTTACATGGAAA+TGG 0.350319 2_2:-28703681 None:intergenic
TGGTTAAGAGCTACTGTCTA+TGG 0.350969 2_2:+28701057 Msa0246910:CDS
TTACTAACCAAAGTATTGTT+TGG 0.352391 2_2:-28702741 None:intergenic
ATATCATTGTACTTACTTAA+GGG 0.353683 2_2:+28703868 Msa0246910:three_prime_UTR
GGATTTAATTTCTTTGTTGT+TGG 0.356145 2_2:+28702864 Msa0246910:CDS
CATGGAAATGGAAATGGAAA+TGG 0.370370 2_2:-28703669 None:intergenic
CTGATGCCTACACCATTAAT+GGG 0.371595 2_2:+28701426 Msa0246910:CDS
AATTTACATGGAAATGGAAA+TGG 0.372179 2_2:-28703675 None:intergenic
TATTTGGTCCTTGACAAGTT+TGG 0.374143 2_2:-28702560 None:intergenic
AGAAAAGGGTTTCTTTAGTA+AGG 0.376132 2_2:-28703631 None:intergenic
TTGCTGTGCCTTCTGGTGGT+TGG 0.376214 2_2:+28702964 Msa0246910:CDS
AGGTCCAGGAAACCCATTAA+TGG 0.377126 2_2:-28701438 None:intergenic
AACTCTGACCTGTTTGAATT+GGG 0.379719 2_2:-28700972 None:intergenic
AACAAAATTAGTATCATTGA+TGG 0.379750 2_2:-28702415 None:intergenic
CAACATTTGGGGAGATCCTT+AGG 0.380323 2_2:-28703578 None:intergenic
AGCATGTAGAATGTGGCATT+TGG 0.380488 2_2:-28702267 None:intergenic
GACAAAGAGGAACTCTCTTC+TGG 0.381182 2_2:+28701019 Msa0246910:CDS
AAAAGCATGGTTACCGTGAA+TGG 0.383433 2_2:+28700498 Msa0246910:CDS
AGGGGACAGATTATTGGTTA+AGG 0.383575 2_2:+28700554 Msa0246910:CDS
TCAACAAGATTAAACCTTGC+AGG 0.384892 2_2:-28702921 None:intergenic
GTACCCTGCCCTGTGAAATA+TGG 0.385825 2_2:-28702297 None:intergenic
CATAAATCCTTAAATTTACA+TGG 0.392188 2_2:-28703687 None:intergenic
TAGAGTTCAGGTTATATTGC+AGG 0.393539 2_2:+28702797 Msa0246910:CDS
TACCGTGAATGGGAAGTTCC+CGG 0.400570 2_2:+28700509 Msa0246910:CDS
AACTATCTTGTACTCATTCA+AGG 0.405635 2_2:+28700139 Msa0246910:five_prime_UTR
GAGAATGGGGACTTCCTTGT+AGG 0.406530 2_2:-28701130 None:intergenic
GTGTAGGCATCAGAAACATT+TGG 0.413299 2_2:-28701416 None:intergenic
GCATGTAGAATGTGGCATTT+GGG 0.416680 2_2:-28702266 None:intergenic
ACTTTGATGTGCACCTTAGT+TGG 0.416806 2_2:+28703492 Msa0246910:CDS
ACCTTCCTCTTAACCTCCCC+TGG 0.418136 2_2:+28700236 Msa0246910:CDS
AAAATCAAAAGGTGTAGAAA+AGG 0.418687 2_2:-28703646 None:intergenic
ACCATATAACACTAGAGTTC+AGG 0.422189 2_2:+28702785 Msa0246910:CDS
GTATAGTTAAACGGCCTTAA+CGG 0.423945 2_2:-28702708 None:intergenic
TTCGACAATTCCACAGTAGC+TGG 0.425573 2_2:+28702321 Msa0246910:CDS
AATTTCTTTGTTGTTGGTCA+AGG 0.431250 2_2:+28702870 Msa0246910:CDS
TTAAGGCCGTTTAACTATAC+AGG 0.432215 2_2:+28702711 Msa0246910:CDS
AACAATACTTTGGTTAGTAA+TGG 0.432888 2_2:+28702744 Msa0246910:CDS
GATAAGATTCATTGTGCCTT+GGG 0.433565 2_2:-28701095 None:intergenic
CAAATCCAGGGGAGGTTAAG+AGG 0.435761 2_2:-28700241 None:intergenic
GTGTACAACTTCACCATTGT+TGG 0.440870 2_2:+28700997 Msa0246910:CDS
TTCATATGTACCAAAGAGAA+TGG 0.444243 2_2:-28701145 None:intergenic
AACGGCCTTAACGGTACAAC+CGG 0.445112 2_2:-28702699 None:intergenic
TGTAGGCATCAGAAACATTT+GGG 0.450843 2_2:-28701415 None:intergenic
GTTGGGGATTAAGGATGGCT+TGG 0.452720 2_2:+28703510 Msa0246910:CDS
AATGAGTACAAGATAGTTGA+AGG 0.454547 2_2:-28700134 None:intergenic
GCAACAGTATTCCTCTCAAC+AGG 0.458149 2_2:-28702945 None:intergenic
AACTCCTCCAAACAATACTT+TGG 0.459111 2_2:+28702734 Msa0246910:CDS
GGATAAGATTCATTGTGCCT+TGG 0.462220 2_2:-28701096 None:intergenic
GGAAAATTGGCAGAGTCAAT+TGG 0.466366 2_2:-28703610 None:intergenic
TCATTGATGGCTGGAAGCAC+AGG 0.471762 2_2:-28702402 None:intergenic
AAAATAGTTCTTACCAATGC+AGG 0.472186 2_2:-28700605 None:intergenic
GGAGTTCCTGTATAGTTAAA+CGG 0.473322 2_2:-28702717 None:intergenic
TCATATGTACCAAAGAGAAT+GGG 0.475158 2_2:-28701144 None:intergenic
TCTGGCATGCTCATTTCTCA+TGG 0.475847 2_2:+28701037 Msa0246910:CDS
AAATCAAAAGGTGTAGAAAA+GGG 0.479134 2_2:-28703645 None:intergenic
TAGTTCCAAAGAAATGTTGT+TGG 0.480695 2_2:-28702653 None:intergenic
ATTATTTCTGCAGATAAGGT+TGG 0.482733 2_2:+28700449 Msa0246910:intron
ATTGCACAAGCTCTGCAGAC+AGG 0.483287 2_2:+28701386 Msa0246910:CDS
ACTCAATGCCCAATTCAAAC+AGG 0.483829 2_2:+28700964 Msa0246910:CDS
AATGGAAATGGAAAATCAAA+AGG 0.487974 2_2:-28703657 None:intergenic
GAAGTAGCCAAAACAAATCC+AGG 0.492773 2_2:-28700254 None:intergenic
TTCACCATTGTTGGACAAAG+AGG 0.495747 2_2:+28701006 Msa0246910:CDS
GGGCATTGAGTTATGTATGA+TGG 0.498399 2_2:-28700952 None:intergenic
TGATACAATGAAAGAAAGAC+TGG 0.498850 2_2:-28703775 None:intergenic
CCTTCTCGTACAACAATTCG+AGG 0.499048 2_2:-28700534 None:intergenic
TGGTCCAAGTATGATGGTAT+TGG 0.499764 2_2:-28702202 None:intergenic
TGCAGAGAGTGAAGTGTGTC+CGG 0.503416 2_2:+28703734 Msa0246910:three_prime_UTR
GTATGGTTGAAACAGGTGTT+TGG 0.506981 2_2:+28703456 Msa0246910:intron
ACCCGGGAACTTCCCATTCA+CGG 0.507216 2_2:-28700511 None:intergenic
GAGCAATTGTAAAGAGGTCC+AGG 0.507787 2_2:-28701452 None:intergenic
CTTCGTAGTGGTTGGTCGGA+TGG 0.508027 2_2:+28700928 Msa0246910:CDS
CGATACCTTGCTGACAATCC+AGG 0.508335 2_2:+28702996 Msa0246910:CDS
CTCGAATTGTTGTACGAGAA+GGG 0.509000 2_2:+28700535 Msa0246910:CDS
TGTTGGAGAAGTGCTATAGA+AGG 0.510179 2_2:-28702636 None:intergenic
CCTCTTTACAATTGCTCCAA+AGG 0.511152 2_2:+28701458 Msa0246910:CDS
AAAGCATGGTTACCGTGAAT+GGG 0.513288 2_2:+28700499 Msa0246910:CDS
AAGGATGGCTTGGATTGTTG+AGG 0.513739 2_2:+28703520 Msa0246910:CDS
CCTTAGTTGGGGATTAAGGA+TGG 0.516631 2_2:+28703505 Msa0246910:CDS
ATTCATTGTGCCTTGGGAGA+AGG 0.517082 2_2:-28701089 None:intergenic
AGAATGGGGACTTCCTTGTA+GGG 0.517590 2_2:-28701129 None:intergenic
TTTGATGTGCACCTTAGTTG+GGG 0.517799 2_2:+28703494 Msa0246910:CDS
ATGGCTTGGATTGTTGAGGA+TGG 0.518174 2_2:+28703524 Msa0246910:CDS
TAGACCATATTTCACAGGGC+AGG 0.518387 2_2:+28702293 Msa0246910:CDS
AAAATTGCATCCTCCTCCTA+AGG 0.524928 2_2:+28703562 Msa0246910:CDS
GACAGATACATACAAATTGA+AGG 0.527305 2_2:+28702050 Msa0246910:intron
CTAGCTAGACCATATTTCAC+AGG 0.529517 2_2:+28702288 Msa0246910:CDS
GATAAAACGCTTGAATCATA+CGG 0.529877 2_2:-28703989 None:intergenic
TACAAATTGAAGGTGAAGCC+AGG 0.530121 2_2:+28702060 Msa0246910:CDS
AGAACATTTGTGGTTTGACC+TGG 0.536892 2_2:-28702222 None:intergenic
CTAACCAAAGTATTGTTTGG+AGG 0.538509 2_2:-28702738 None:intergenic
TTGCACAAGCTCTGCAGACA+GGG 0.538737 2_2:+28701387 Msa0246910:CDS
ACCGTGAATGGGAAGTTCCC+GGG 0.543276 2_2:+28700510 Msa0246910:CDS
GAGTCCAATACCATCATACT+TGG 0.543746 2_2:+28702198 Msa0246910:CDS
ATGCTAGTATTGACCCTGCA+AGG 0.544490 2_2:+28702907 Msa0246910:CDS
CCTCGAATTGTTGTACGAGA+AGG 0.546915 2_2:+28700534 Msa0246910:CDS
ACTGTTGCTGTGCCTTCTGG+TGG 0.553231 2_2:+28702960 Msa0246910:CDS
CCTTTGGAGCAATTGTAAAG+AGG 0.556173 2_2:-28701458 None:intergenic
TTGGGGAGATCCTTAGGAGG+AGG 0.561208 2_2:-28703572 None:intergenic
TCTGCAGATAAGGTTGGCAA+AGG 0.561585 2_2:+28700455 Msa0246910:intron
TGTCACTGTTCCATTATCAA+AGG 0.562747 2_2:+28700184 Msa0246910:CDS
CGTACAACAATTCGAGGACC+CGG 0.566305 2_2:-28700528 None:intergenic
CCATCCTTAATCCCCAACTA+AGG 0.569779 2_2:-28703505 None:intergenic
GAATCATCATATACTAGTGC+CGG 0.571850 2_2:-28703753 None:intergenic
TGAGACAACTTCGTAGTGGT+TGG 0.574177 2_2:+28700920 Msa0246910:CDS
TTAAATCCATGAAGATGCAA+AGG 0.575251 2_2:-28702849 None:intergenic
TTCTAAAATTCCAGCTACTG+TGG 0.577452 2_2:-28702331 None:intergenic
AGGAAACCCATTAATGGTGT+AGG 0.579472 2_2:-28701432 None:intergenic
AAATTAGTATCATTGATGGC+TGG 0.579956 2_2:-28702411 None:intergenic
CAAACAATATCAGCCTGCAT+TGG 0.581284 2_2:+28700592 Msa0246910:CDS
AATCACCTGGATTGTCAGCA+AGG 0.581837 2_2:-28703001 None:intergenic
TTGACCTGGTCCAAGTATGA+TGG 0.586103 2_2:-28702208 None:intergenic
GACCTCTCATCCTTCTCCCA+AGG 0.596454 2_2:+28701079 Msa0246910:CDS
TCCAGGGGAGGTTAAGAGGA+AGG 0.601150 2_2:-28700237 None:intergenic
ACAACTTCGTAGTGGTTGGT+CGG 0.604046 2_2:+28700924 Msa0246910:CDS
AAGTAGCCAAAACAAATCCA+GGG 0.606149 2_2:-28700253 None:intergenic
ATCTTGTTGATCCTGTTGAG+AGG 0.606779 2_2:+28702934 Msa0246910:CDS
AGTTCCTCTTTGTCCAACAA+TGG 0.608427 2_2:-28701010 None:intergenic
AGACCATATTTCACAGGGCA+GGG 0.613998 2_2:+28702294 Msa0246910:CDS
CAACAAGATTAAACCTTGCA+GGG 0.615814 2_2:-28702920 None:intergenic
TCGAATTGTTGTACGAGAAG+GGG 0.619585 2_2:+28700536 Msa0246910:CDS
CATTTGGGGAGATCCTTAGG+AGG 0.640212 2_2:-28703575 None:intergenic
TGCACAGTATATGCAAAATG+TGG 0.642341 2_2:-28703715 None:intergenic
ATCGCAACCCAACCACCAGA+AGG 0.645168 2_2:-28702972 None:intergenic
TGCCTTGGGAGAAGGATGAG+AGG 0.645507 2_2:-28701081 None:intergenic
ATGATGGTATTGGACTCGAA+TGG 0.647830 2_2:-28702192 None:intergenic
CATATGTACCAAAGAGAATG+GGG 0.650276 2_2:-28701143 None:intergenic
TGGACTAGTTCCTAGTCCAA+CGG 0.652330 2_2:-28702526 None:intergenic
GACAAATATGCATAGGTGAG+TGG 0.653174 2_2:+28701339 Msa0246910:intron
AGCCAAAACAAATCCAGGGG+AGG 0.654034 2_2:-28700249 None:intergenic
GTACAACAATTCGAGGACCC+GGG 0.658530 2_2:-28700527 None:intergenic
AAATATGCATAGGTGAGTGG+TGG 0.659012 2_2:+28701342 Msa0246910:intron
TCTAGCTAGCATGTAGAATG+TGG 0.659413 2_2:-28702274 None:intergenic
TGCACAAGCTCTGCAGACAG+GGG 0.660853 2_2:+28701388 Msa0246910:CDS
TAAGGTTGGCAAAGGTTACG+AGG 0.665174 2_2:+28700463 Msa0246910:CDS
AAGATGCATCTGCTTCGACA+AGG 0.668532 2_2:-28702161 None:intergenic
GCACAAGCTCTGCAGACAGG+GGG 0.670081 2_2:+28701389 Msa0246910:CDS
AACTAAGGTGCACATCAAAG+TGG 0.677837 2_2:-28703490 None:intergenic
TAGCTAGACCATATTTCACA+GGG 0.683532 2_2:+28702289 Msa0246910:CDS
AATACCATCATACTTGGACC+AGG 0.697209 2_2:+28702204 Msa0246910:CDS
TAGTAATGGAACAAAGACAG+TGG 0.719057 2_2:+28702758 Msa0246910:CDS
AGTAGCCAAAACAAATCCAG+GGG 0.722267 2_2:-28700252 None:intergenic
AGGGGAGGTTAAGAGGAAGG+TGG 0.734018 2_2:-28700234 None:intergenic
GGTGTAGTAATCAACATTTG+GGG 0.734375 2_2:-28703589 None:intergenic
GGTGTGAGACAACTTCGTAG+TGG 0.741558 2_2:+28700916 Msa0246910:CDS
TATCTTGTACTCATTCAAGG+TGG 0.745577 2_2:+28700142 Msa0246910:five_prime_UTR

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! TTTATTATAAATTTATACTG+AGG - chr2_2:28701179-28701198 None:intergenic 10.0%
!! ATAAATAATTTATTATACTC+TGG + chr2_2:28701194-28701213 Msa0246910:intron 10.0%
!!! CATATAAATAACTTTTTTTT+AGG - chr2_2:28703364-28703383 None:intergenic 10.0%
!!! AACTTTTTATTCAAAAAAAA+AGG + chr2_2:28703899-28703918 Msa0246910:three_prime_UTR 10.0%
!!! ACTTTTTATTCAAAAAAAAA+GGG + chr2_2:28703900-28703919 Msa0246910:three_prime_UTR 10.0%
!! TTAAGTGATAAATATTTGAA+AGG - chr2_2:28700348-28700367 None:intergenic 15.0%
!! AAAATAGTATATGATATGAA+AGG + chr2_2:28701292-28701311 Msa0246910:intron 15.0%
!!! TTTTTATTTGTTTGATCATT+TGG - chr2_2:28702366-28702385 None:intergenic 15.0%
!! TTTGAATTTGAACTATTATT+TGG - chr2_2:28702579-28702598 None:intergenic 15.0%
!!! ATTGACTTAATTATTTAATC+TGG - chr2_2:28703276-28703295 None:intergenic 15.0%
!!! ATGTATTTTTCTCATATTTT+TGG + chr2_2:28703947-28703966 Msa0246910:three_prime_UTR 15.0%
!! ACTACATATGTTATACAATT+TGG - chr2_2:28700390-28700409 None:intergenic 20.0%
!!! TTTTATTATTTCTGCAGATA+AGG + chr2_2:28700445-28700464 Msa0246910:intron 20.0%
!! AATAATTCATTTCATGCATA+TGG - chr2_2:28700844-28700863 None:intergenic 20.0%
!! TGTACTAATATTGACAATAA+TGG + chr2_2:28700863-28700882 Msa0246910:intron 20.0%
!!! AAATTAGTTTGTAAAATGAG+AGG - chr2_2:28701934-28701953 None:intergenic 20.0%
!!! TTTTTGAAAGATGAATTGAT+TGG + chr2_2:28702010-28702029 Msa0246910:intron 20.0%
!!! AGTTTTTAATAGAACATTTG+TGG - chr2_2:28702235-28702254 None:intergenic 20.0%
!! AACAAAATTAGTATCATTGA+TGG - chr2_2:28702418-28702437 None:intergenic 20.0%
!!! AAGTTTTTGTCAATAGTTTT+AGG - chr2_2:28702498-28702517 None:intergenic 20.0%
!!! AACTAATAATGGTTTTACAA+CGG + chr2_2:28702671-28702690 Msa0246910:CDS 20.0%
!! AATTATTTAATCTGGTCTAT+TGG - chr2_2:28703268-28703287 None:intergenic 20.0%
!! CATAAATCCTTAAATTTACA+TGG - chr2_2:28703690-28703709 None:intergenic 20.0%
!! CATGTAAATTTAAGGATTTA+TGG + chr2_2:28703688-28703707 Msa0246910:three_prime_UTR 20.0%
!! AATATCATTGTACTTACTTA+AGG + chr2_2:28703867-28703886 Msa0246910:three_prime_UTR 20.0%
!! ATATCATTGTACTTACTTAA+GGG + chr2_2:28703868-28703887 Msa0246910:three_prime_UTR 20.0%
! CATATGTTATACAATTTGGT+AGG - chr2_2:28700386-28700405 None:intergenic 25.0%
!!! AAACTAAGTGTTGAGAAAAT+AGG + chr2_2:28700691-28700710 Msa0246910:intron 25.0%
! ATATTGTATGTTGTACATCA+TGG + chr2_2:28700803-28700822 Msa0246910:intron 25.0%
!!! TGGTATTTTATATTTCTAGC+TGG + chr2_2:28701214-28701233 Msa0246910:intron 25.0%
!!! CTGGAGTTTAAACTTTTTTA+AGG + chr2_2:28701233-28701252 Msa0246910:intron 25.0%
! TATCTTTGACAAATATGCAT+AGG + chr2_2:28701332-28701351 Msa0246910:intron 25.0%
!! AACATTTTCTTTATCCTAAG+TGG - chr2_2:28701886-28701905 None:intergenic 25.0%
! GAATAATGACTAGAATTGAT+TGG + chr2_2:28701980-28701999 Msa0246910:intron 25.0%
!!! ATTGGTTTGTTATCATATCA+TGG + chr2_2:28702028-28702047 Msa0246910:intron 25.0%
!!! AAAAACTTTTTCTTTACCGT+TGG + chr2_2:28702510-28702529 Msa0246910:CDS 25.0%
!!! CATTTCTTTGGAACTAATAA+TGG + chr2_2:28702660-28702679 Msa0246910:CDS 25.0%
!!! TTACTAACCAAAGTATTGTT+TGG - chr2_2:28702744-28702763 None:intergenic 25.0%
! AACAATACTTTGGTTAGTAA+TGG + chr2_2:28702744-28702763 Msa0246910:CDS 25.0%
!! GGATTTAATTTCTTTGTTGT+TGG + chr2_2:28702864-28702883 Msa0246910:CDS 25.0%
! AGCTTATGAAATCAGTTTAT+AGG - chr2_2:28703137-28703156 None:intergenic 25.0%
!!! ATCTATTTGCTATGAATGTA+TGG + chr2_2:28703439-28703458 Msa0246910:intron 25.0%
! GTTTCTTTAGTAAGGAAAAT+TGG - chr2_2:28703626-28703645 None:intergenic 25.0%
!! AAATCAAAAGGTGTAGAAAA+GGG - chr2_2:28703648-28703667 None:intergenic 25.0%
!! AAAATCAAAAGGTGTAGAAA+AGG - chr2_2:28703649-28703668 None:intergenic 25.0%
! AATGGAAATGGAAAATCAAA+AGG - chr2_2:28703660-28703679 None:intergenic 25.0%
! AATTTACATGGAAATGGAAA+TGG - chr2_2:28703678-28703697 None:intergenic 25.0%
! TCCATTTCCATGTAAATTTA+AGG + chr2_2:28703680-28703699 Msa0246910:three_prime_UTR 25.0%
! TCCTTAAATTTACATGGAAA+TGG - chr2_2:28703684-28703703 None:intergenic 25.0%
!!! TTTTTGGTCACTTGAAAATT+AGG - chr2_2:28703814-28703833 None:intergenic 25.0%
! TTCAAGTGACCAAAAAATAA+AGG + chr2_2:28703818-28703837 Msa0246910:three_prime_UTR 25.0%
AATGAGTACAAGATAGTTGA+AGG - chr2_2:28700137-28700156 None:intergenic 30.0%
AACTATCTTGTACTCATTCA+AGG + chr2_2:28700139-28700158 Msa0246910:five_prime_UTR 30.0%
!! ATTATTTCTGCAGATAAGGT+TGG + chr2_2:28700449-28700468 Msa0246910:intron 30.0%
AAAATAGTTCTTACCAATGC+AGG - chr2_2:28700608-28700627 None:intergenic 30.0%
! TGTGAGTTTTGCAGTTAATT+AGG - chr2_2:28700670-28700689 None:intergenic 30.0%
TGAGAAAATAGGATTTGAAC+TGG + chr2_2:28700702-28700721 Msa0246910:intron 30.0%
TGCATGCATGATAAAAAAAC+AGG + chr2_2:28700890-28700909 Msa0246910:intron 30.0%
TCATATGTACCAAAGAGAAT+GGG - chr2_2:28701147-28701166 None:intergenic 30.0%
TTCATATGTACCAAAGAGAA+TGG - chr2_2:28701148-28701167 None:intergenic 30.0%
GACAGATACATACAAATTGA+AGG + chr2_2:28702050-28702069 Msa0246910:intron 30.0%
! CGTAATAGATATGTTTTTCC+TGG - chr2_2:28702081-28702100 None:intergenic 30.0%
! AAATTAGTATCATTGATGGC+TGG - chr2_2:28702414-28702433 None:intergenic 30.0%
!!! CTTGACAAGTTTGGTTTTTT+GGG - chr2_2:28702554-28702573 None:intergenic 30.0%
TAGTTCCAAAGAAATGTTGT+TGG - chr2_2:28702656-28702675 None:intergenic 30.0%
TTAAATCCATGAAGATGCAA+AGG - chr2_2:28702852-28702871 None:intergenic 30.0%
!! AATTTCTTTGTTGTTGGTCA+AGG + chr2_2:28702870-28702889 Msa0246910:CDS 30.0%
!! CTTATTTTCGTAAGTTCTCT+AGG - chr2_2:28703162-28703181 None:intergenic 30.0%
TATGAATGTATGGTTGAAAC+AGG + chr2_2:28703449-28703468 Msa0246910:intron 30.0%
TGGTGTAGTAATCAACATTT+GGG - chr2_2:28703593-28703612 None:intergenic 30.0%
TTGGTGTAGTAATCAACATT+TGG - chr2_2:28703594-28703613 None:intergenic 30.0%
AGAAAAGGGTTTCTTTAGTA+AGG - chr2_2:28703634-28703653 None:intergenic 30.0%
TGATACAATGAAAGAAAGAC+TGG - chr2_2:28703778-28703797 None:intergenic 30.0%
AAGTGACCAAAAAATAAAGG+TGG + chr2_2:28703821-28703840 Msa0246910:three_prime_UTR 30.0%
AGTGACCAAAAAATAAAGGT+GGG + chr2_2:28703822-28703841 Msa0246910:three_prime_UTR 30.0%
GATAAAACGCTTGAATCATA+CGG - chr2_2:28703992-28704011 None:intergenic 30.0%
TATCTTGTACTCATTCAAGG+TGG + chr2_2:28700142-28700161 Msa0246910:five_prime_UTR 35.0%
TGTCACTGTTCCATTATCAA+AGG + chr2_2:28700184-28700203 Msa0246910:CDS 35.0%
GCAAAAAGAGCCTTTGATAA+TGG - chr2_2:28700197-28700216 None:intergenic 35.0%
AAGTAGCCAAAACAAATCCA+GGG - chr2_2:28700256-28700275 None:intergenic 35.0%
CAACGACATTTGATATGCTT+GGG - chr2_2:28700730-28700749 None:intergenic 35.0%
ACAACGACATTTGATATGCT+TGG - chr2_2:28700731-28700750 None:intergenic 35.0%
TGTACATCATGGATCTAGTT+AGG + chr2_2:28700814-28700833 Msa0246910:intron 35.0%
GCATGATAAAAAAACAGGCA+TGG + chr2_2:28700895-28700914 Msa0246910:intron 35.0%
AACTCTGACCTGTTTGAATT+GGG - chr2_2:28700975-28700994 None:intergenic 35.0%
TAACTCTGACCTGTTTGAAT+TGG - chr2_2:28700976-28700995 None:intergenic 35.0%
GATAAGATTCATTGTGCCTT+GGG - chr2_2:28701098-28701117 None:intergenic 35.0%
! TCCTTTTCAAAAACCCTACA+AGG + chr2_2:28701116-28701135 Msa0246910:CDS 35.0%
!! TCCTTGTAGGGTTTTTGAAA+AGG - chr2_2:28701120-28701139 None:intergenic 35.0%
CATATGTACCAAAGAGAATG+GGG - chr2_2:28701146-28701165 None:intergenic 35.0%
TGTAGGCATCAGAAACATTT+GGG - chr2_2:28701418-28701437 None:intergenic 35.0%
GAAAAAACCAAGTTCCACTT+AGG + chr2_2:28701869-28701888 Msa0246910:intron 35.0%
TCTTTATCCTAAGTGGAACT+TGG - chr2_2:28701879-28701898 None:intergenic 35.0%
AATGTGGCATTTGGGTAATT+TGG - chr2_2:28702261-28702280 None:intergenic 35.0%
TAGCTAGACCATATTTCACA+GGG + chr2_2:28702289-28702308 Msa0246910:CDS 35.0%
!! TTCTAAAATTCCAGCTACTG+TGG - chr2_2:28702334-28702353 None:intergenic 35.0%
!!! GAAGCACAGGTTTTAGAATA+TGG - chr2_2:28702392-28702411 None:intergenic 35.0%
!!! TAGTTTTAGGCACATTTGCA+GGG - chr2_2:28702485-28702504 None:intergenic 35.0%
!!! ATAGTTTTAGGCACATTTGC+AGG - chr2_2:28702486-28702505 None:intergenic 35.0%
! TTTTTCTTTACCGTTGGACT+AGG + chr2_2:28702516-28702535 Msa0246910:CDS 35.0%
!!! CCTTGACAAGTTTGGTTTTT+TGG - chr2_2:28702555-28702574 None:intergenic 35.0%
CCAAAAAACCAAACTTGTCA+AGG + chr2_2:28702552-28702571 Msa0246910:CDS 35.0%
TATTTGGTCCTTGACAAGTT+TGG - chr2_2:28702563-28702582 None:intergenic 35.0%
CTTCTCCAACAACATTTCTT+TGG + chr2_2:28702648-28702667 Msa0246910:CDS 35.0%
!! TGGTTTTACAACGGATTTTC+CGG + chr2_2:28702680-28702699 Msa0246910:CDS 35.0%
GTATAGTTAAACGGCCTTAA+CGG - chr2_2:28702711-28702730 None:intergenic 35.0%
TTAAGGCCGTTTAACTATAC+AGG + chr2_2:28702711-28702730 Msa0246910:CDS 35.0%
GGAGTTCCTGTATAGTTAAA+CGG - chr2_2:28702720-28702739 None:intergenic 35.0%
AACTCCTCCAAACAATACTT+TGG + chr2_2:28702734-28702753 Msa0246910:CDS 35.0%
!! CTAACCAAAGTATTGTTTGG+AGG - chr2_2:28702741-28702760 None:intergenic 35.0%
! TAGTAATGGAACAAAGACAG+TGG + chr2_2:28702758-28702777 Msa0246910:CDS 35.0%
ACCATATAACACTAGAGTTC+AGG + chr2_2:28702785-28702804 Msa0246910:CDS 35.0%
ACCTGAACTCTAGTGTTATA+TGG - chr2_2:28702789-28702808 None:intergenic 35.0%
! TAGAGTTCAGGTTATATTGC+AGG + chr2_2:28702797-28702816 Msa0246910:CDS 35.0%
! ATTGCAGGATACAAGCATTT+TGG + chr2_2:28702812-28702831 Msa0246910:CDS 35.0%
! TTGCAGGATACAAGCATTTT+GGG + chr2_2:28702813-28702832 Msa0246910:CDS 35.0%
!!! TTTGTTGTTGGTCAAGGTTT+TGG + chr2_2:28702876-28702895 Msa0246910:CDS 35.0%
CAACAAGATTAAACCTTGCA+GGG - chr2_2:28702923-28702942 None:intergenic 35.0%
TCAACAAGATTAAACCTTGC+AGG - chr2_2:28702924-28702943 None:intergenic 35.0%
! TGATTGACTATGGTTCTGTT+TGG + chr2_2:28703019-28703038 Msa0246910:intron 35.0%
!! GGAGGATGCAATTTTTGATT+AGG - chr2_2:28703557-28703576 None:intergenic 35.0%
GGTGTAGTAATCAACATTTG+GGG - chr2_2:28703592-28703611 None:intergenic 35.0%
CATGGAAATGGAAATGGAAA+TGG - chr2_2:28703672-28703691 None:intergenic 35.0%
TGCACAGTATATGCAAAATG+TGG - chr2_2:28703718-28703737 None:intergenic 35.0%
GAATCATCATATACTAGTGC+CGG - chr2_2:28703756-28703775 None:intergenic 35.0%
!!! AACCACCCACCTTTATTTTT+TGG - chr2_2:28703830-28703849 None:intergenic 35.0%
AGTAGCCAAAACAAATCCAG+GGG - chr2_2:28700255-28700274 None:intergenic 40.0%
GAAGTAGCCAAAACAAATCC+AGG - chr2_2:28700257-28700276 None:intergenic 40.0%
!!! GTTTTGGCTACTTCACAACA+TGG + chr2_2:28700263-28700282 Msa0246910:CDS 40.0%
! AAAAGCATGGTTACCGTGAA+TGG + chr2_2:28700498-28700517 Msa0246910:CDS 40.0%
AAAGCATGGTTACCGTGAAT+GGG + chr2_2:28700499-28700518 Msa0246910:CDS 40.0%
CTCGAATTGTTGTACGAGAA+GGG + chr2_2:28700535-28700554 Msa0246910:CDS 40.0%
TCGAATTGTTGTACGAGAAG+GGG + chr2_2:28700536-28700555 Msa0246910:CDS 40.0%
! AGGGGACAGATTATTGGTTA+AGG + chr2_2:28700554-28700573 Msa0246910:CDS 40.0%
CAATGCAGGCTGATATTGTT+TGG - chr2_2:28700594-28700613 None:intergenic 40.0%
CAAACAATATCAGCCTGCAT+TGG + chr2_2:28700592-28700611 Msa0246910:CDS 40.0%
GTGTGTGAGAGTAAAACTCA+TGG - chr2_2:28700762-28700781 None:intergenic 40.0%
!! GGGCATTGAGTTATGTATGA+TGG - chr2_2:28700955-28700974 None:intergenic 40.0%
ACTCAATGCCCAATTCAAAC+AGG + chr2_2:28700964-28700983 Msa0246910:CDS 40.0%
GTGTACAACTTCACCATTGT+TGG + chr2_2:28700997-28701016 Msa0246910:CDS 40.0%
TTCACCATTGTTGGACAAAG+AGG + chr2_2:28701006-28701025 Msa0246910:CDS 40.0%
AGTTCCTCTTTGTCCAACAA+TGG - chr2_2:28701013-28701032 None:intergenic 40.0%
TGGTTAAGAGCTACTGTCTA+TGG + chr2_2:28701057-28701076 Msa0246910:CDS 40.0%
GGATAAGATTCATTGTGCCT+TGG - chr2_2:28701099-28701118 None:intergenic 40.0%
GACAAATATGCATAGGTGAG+TGG + chr2_2:28701339-28701358 Msa0246910:intron 40.0%
AAATATGCATAGGTGAGTGG+TGG + chr2_2:28701342-28701361 Msa0246910:intron 40.0%
GTGTAGGCATCAGAAACATT+TGG - chr2_2:28701419-28701438 None:intergenic 40.0%
TCTGATGCCTACACCATTAA+TGG + chr2_2:28701425-28701444 Msa0246910:CDS 40.0%
CTGATGCCTACACCATTAAT+GGG + chr2_2:28701426-28701445 Msa0246910:CDS 40.0%
AGGAAACCCATTAATGGTGT+AGG - chr2_2:28701435-28701454 None:intergenic 40.0%
!! TACACCATTAATGGGTTTCC+TGG + chr2_2:28701434-28701453 Msa0246910:CDS 40.0%
!! CCTTTGGAGCAATTGTAAAG+AGG - chr2_2:28701461-28701480 None:intergenic 40.0%
CCTCTTTACAATTGCTCCAA+AGG + chr2_2:28701458-28701477 Msa0246910:CDS 40.0%
ACGTGCAGCTTGTATATAAC+TGG + chr2_2:28701484-28701503 Msa0246910:intron 40.0%
TACAAATTGAAGGTGAAGCC+AGG + chr2_2:28702060-28702079 Msa0246910:CDS 40.0%
! ATGATGGTATTGGACTCGAA+TGG - chr2_2:28702195-28702214 None:intergenic 40.0%
GAGTCCAATACCATCATACT+TGG + chr2_2:28702198-28702217 Msa0246910:CDS 40.0%
TGGTCCAAGTATGATGGTAT+TGG - chr2_2:28702205-28702224 None:intergenic 40.0%
AATACCATCATACTTGGACC+AGG + chr2_2:28702204-28702223 Msa0246910:CDS 40.0%
AGAACATTTGTGGTTTGACC+TGG - chr2_2:28702225-28702244 None:intergenic 40.0%
GCATGTAGAATGTGGCATTT+GGG - chr2_2:28702269-28702288 None:intergenic 40.0%
AGCATGTAGAATGTGGCATT+TGG - chr2_2:28702270-28702289 None:intergenic 40.0%
TCTAGCTAGCATGTAGAATG+TGG - chr2_2:28702277-28702296 None:intergenic 40.0%
CTAGCTAGACCATATTTCAC+AGG + chr2_2:28702288-28702307 Msa0246910:CDS 40.0%
!!! CAAGTTTGGTTTTTTGGGCA+TGG - chr2_2:28702549-28702568 None:intergenic 40.0%
!! TGTTGGAGAAGTGCTATAGA+AGG - chr2_2:28702639-28702658 None:intergenic 40.0%
! ATTTTCCGGTTGTACCGTTA+AGG + chr2_2:28702694-28702713 Msa0246910:CDS 40.0%
AGTCATCCTTTGCATCTTCA+TGG + chr2_2:28702843-28702862 Msa0246910:CDS 40.0%
ATCTTGTTGATCCTGTTGAG+AGG + chr2_2:28702934-28702953 Msa0246910:CDS 40.0%
ACAATCCAGGTGATTGACTA+TGG + chr2_2:28703009-28703028 Msa0246910:intron 40.0%
GTATGGTTGAAACAGGTGTT+TGG + chr2_2:28703456-28703475 Msa0246910:intron 40.0%
AACTAAGGTGCACATCAAAG+TGG - chr2_2:28703493-28703512 None:intergenic 40.0%
ACTTTGATGTGCACCTTAGT+TGG + chr2_2:28703492-28703511 Msa0246910:CDS 40.0%
CTTTGATGTGCACCTTAGTT+GGG + chr2_2:28703493-28703512 Msa0246910:CDS 40.0%
TTTGATGTGCACCTTAGTTG+GGG + chr2_2:28703494-28703513 Msa0246910:CDS 40.0%
AAAATTGCATCCTCCTCCTA+AGG + chr2_2:28703562-28703581 Msa0246910:CDS 40.0%
GGAAAATTGGCAGAGTCAAT+TGG - chr2_2:28703613-28703632 None:intergenic 40.0%
GACCAAAAAATAAAGGTGGG+TGG + chr2_2:28703825-28703844 Msa0246910:three_prime_UTR 40.0%
!! AACCTCCCCTGGATTTGTTT+TGG + chr2_2:28700247-28700266 Msa0246910:CDS 45.0%
TCTGCAGATAAGGTTGGCAA+AGG + chr2_2:28700455-28700474 Msa0246910:intron 45.0%
TAAGGTTGGCAAAGGTTACG+AGG + chr2_2:28700463-28700482 Msa0246910:CDS 45.0%
!! CGGTAACCATGCTTTTTGTG+TGG - chr2_2:28700494-28700513 None:intergenic 45.0%
CCTTCTCGTACAACAATTCG+AGG - chr2_2:28700537-28700556 None:intergenic 45.0%
CCTCGAATTGTTGTACGAGA+AGG + chr2_2:28700534-28700553 Msa0246910:CDS 45.0%
ACGAGAAGGGGACAGATTAT+TGG + chr2_2:28700548-28700567 Msa0246910:CDS 45.0%
TGAGACAACTTCGTAGTGGT+TGG + chr2_2:28700920-28700939 Msa0246910:CDS 45.0%
ACAACTTCGTAGTGGTTGGT+CGG + chr2_2:28700924-28700943 Msa0246910:CDS 45.0%
GACAAAGAGGAACTCTCTTC+TGG + chr2_2:28701019-28701038 Msa0246910:CDS 45.0%
TCTGGCATGCTCATTTCTCA+TGG + chr2_2:28701037-28701056 Msa0246910:CDS 45.0%
ATTCATTGTGCCTTGGGAGA+AGG - chr2_2:28701092-28701111 None:intergenic 45.0%
AGAATGGGGACTTCCTTGTA+GGG - chr2_2:28701132-28701151 None:intergenic 45.0%
AAGGAAGTCCCCATTCTCTT+TGG + chr2_2:28701135-28701154 Msa0246910:CDS 45.0%
GCTTGTGCAATTACAGCTTC+AGG - chr2_2:28701377-28701396 None:intergenic 45.0%
AGGTCCAGGAAACCCATTAA+TGG - chr2_2:28701441-28701460 None:intergenic 45.0%
GAGCAATTGTAAAGAGGTCC+AGG - chr2_2:28701455-28701474 None:intergenic 45.0%
AAGATGCATCTGCTTCGACA+AGG - chr2_2:28702164-28702183 None:intergenic 45.0%
TTGACCTGGTCCAAGTATGA+TGG - chr2_2:28702211-28702230 None:intergenic 45.0%
TAGACCATATTTCACAGGGC+AGG + chr2_2:28702293-28702312 Msa0246910:CDS 45.0%
AGACCATATTTCACAGGGCA+GGG + chr2_2:28702294-28702313 Msa0246910:CDS 45.0%
TTCGACAATTCCACAGTAGC+TGG + chr2_2:28702321-28702340 Msa0246910:CDS 45.0%
!! GGCACATTTGCAGGGTATTT+TGG - chr2_2:28702477-28702496 None:intergenic 45.0%
!! TGGACTAGTTCCTAGTCCAA+CGG - chr2_2:28702529-28702548 None:intergenic 45.0%
!! ATGCTAGTATTGACCCTGCA+AGG + chr2_2:28702907-28702926 Msa0246910:CDS 45.0%
GCAACAGTATTCCTCTCAAC+AGG - chr2_2:28702948-28702967 None:intergenic 45.0%
AATACTGTTGCTGTGCCTTC+TGG + chr2_2:28702957-28702976 Msa0246910:CDS 45.0%
AATCACCTGGATTGTCAGCA+AGG - chr2_2:28703004-28703023 None:intergenic 45.0%
CAGAACCATAGTCAATCACC+TGG - chr2_2:28703017-28703036 None:intergenic 45.0%
TGCACCTTAGTTGGGGATTA+AGG + chr2_2:28703501-28703520 Msa0246910:CDS 45.0%
CCATCCTTAATCCCCAACTA+AGG - chr2_2:28703508-28703527 None:intergenic 45.0%
CCTTAGTTGGGGATTAAGGA+TGG + chr2_2:28703505-28703524 Msa0246910:CDS 45.0%
! AAGGATGGCTTGGATTGTTG+AGG + chr2_2:28703520-28703539 Msa0246910:CDS 45.0%
! ATGGCTTGGATTGTTGAGGA+TGG + chr2_2:28703524-28703543 Msa0246910:CDS 45.0%
CAACATTTGGGGAGATCCTT+AGG - chr2_2:28703581-28703600 None:intergenic 45.0%
!! TAAAATATTATTAATTAAAG+TGG - chr2_2:28701834-28701853 None:intergenic 5.0%
CAAATCCAGGGGAGGTTAAG+AGG - chr2_2:28700244-28700263 None:intergenic 50.0%
AGCCAAAACAAATCCAGGGG+AGG - chr2_2:28700252-28700271 None:intergenic 50.0%
GCTGTGCCACACAAAAAGCA+TGG + chr2_2:28700485-28700504 Msa0246910:CDS 50.0%
TACCGTGAATGGGAAGTTCC+CGG + chr2_2:28700509-28700528 Msa0246910:CDS 50.0%
GTACAACAATTCGAGGACCC+GGG - chr2_2:28700530-28700549 None:intergenic 50.0%
CGTACAACAATTCGAGGACC+CGG - chr2_2:28700531-28700550 None:intergenic 50.0%
GGTGTGAGACAACTTCGTAG+TGG + chr2_2:28700916-28700935 Msa0246910:CDS 50.0%
GAGAATGGGGACTTCCTTGT+AGG - chr2_2:28701133-28701152 None:intergenic 50.0%
ATTGCACAAGCTCTGCAGAC+AGG + chr2_2:28701386-28701405 Msa0246910:CDS 50.0%
TTGCACAAGCTCTGCAGACA+GGG + chr2_2:28701387-28701406 Msa0246910:CDS 50.0%
ACAAGCTGCACGTGTACCTT+TGG - chr2_2:28701477-28701496 None:intergenic 50.0%
GTACCCTGCCCTGTGAAATA+TGG - chr2_2:28702300-28702319 None:intergenic 50.0%
! TCATTGATGGCTGGAAGCAC+AGG - chr2_2:28702405-28702424 None:intergenic 50.0%
AACGGCCTTAACGGTACAAC+CGG - chr2_2:28702702-28702721 None:intergenic 50.0%
CGATACCTTGCTGACAATCC+AGG + chr2_2:28702996-28703015 Msa0246910:CDS 50.0%
GTTGGGGATTAAGGATGGCT+TGG + chr2_2:28703510-28703529 Msa0246910:CDS 50.0%
CATTTGGGGAGATCCTTAGG+AGG - chr2_2:28703578-28703597 None:intergenic 50.0%
TGCAGAGAGTGAAGTGTGTC+CGG + chr2_2:28703734-28703753 Msa0246910:three_prime_UTR 50.0%
AGGGGAGGTTAAGAGGAAGG+TGG - chr2_2:28700237-28700256 None:intergenic 55.0%
ACCTTCCTCTTAACCTCCCC+TGG + chr2_2:28700236-28700255 Msa0246910:CDS 55.0%
TCCAGGGGAGGTTAAGAGGA+AGG - chr2_2:28700240-28700259 None:intergenic 55.0%
ACCGTGAATGGGAAGTTCCC+GGG + chr2_2:28700510-28700529 Msa0246910:CDS 55.0%
ACCCGGGAACTTCCCATTCA+CGG - chr2_2:28700514-28700533 None:intergenic 55.0%
! CTTCGTAGTGGTTGGTCGGA+TGG + chr2_2:28700928-28700947 Msa0246910:CDS 55.0%
GACCTCTCATCCTTCTCCCA+AGG + chr2_2:28701079-28701098 Msa0246910:CDS 55.0%
TGCCTTGGGAGAAGGATGAG+AGG - chr2_2:28701084-28701103 None:intergenic 55.0%
TGCACAAGCTCTGCAGACAG+GGG + chr2_2:28701388-28701407 Msa0246910:CDS 55.0%
ACTGTTGCTGTGCCTTCTGG+TGG + chr2_2:28702960-28702979 Msa0246910:CDS 55.0%
TTGCTGTGCCTTCTGGTGGT+TGG + chr2_2:28702964-28702983 Msa0246910:CDS 55.0%
TGCTGTGCCTTCTGGTGGTT+GGG + chr2_2:28702965-28702984 Msa0246910:CDS 55.0%
ATCGCAACCCAACCACCAGA+AGG - chr2_2:28702975-28702994 None:intergenic 55.0%
TTGGGGAGATCCTTAGGAGG+AGG - chr2_2:28703575-28703594 None:intergenic 55.0%
GCACAAGCTCTGCAGACAGG+GGG + chr2_2:28701389-28701408 Msa0246910:CDS 60.0%
CAAGCTCTGCAGACAGGGGG+TGG + chr2_2:28701392-28701411 Msa0246910:CDS 65.0%
Chromosome Type Strat End Strand Name
chr2_2 gene 28700109 28704025 28700109 ID=Msa0246910;Name=Msa0246910
chr2_2 mRNA 28700109 28704025 28700109 ID=Msa0246910-mRNA-1;Parent=Msa0246910;Name=Msa0246910-mRNA-1;_AED=0.03;_eAED=0.03;_QI=58|1|1|1|1|1|6|424|587
chr2_2 exon 28700109 28700313 28700109 ID=Msa0246910-mRNA-1:exon:10262;Parent=Msa0246910-mRNA-1
chr2_2 exon 28700462 28700613 28700462 ID=Msa0246910-mRNA-1:exon:10263;Parent=Msa0246910-mRNA-1
chr2_2 exon 28700912 28701156 28700912 ID=Msa0246910-mRNA-1:exon:10264;Parent=Msa0246910-mRNA-1
chr2_2 exon 28701354 28701479 28701354 ID=Msa0246910-mRNA-1:exon:10265;Parent=Msa0246910-mRNA-1
chr2_2 exon 28702055 28703017 28702055 ID=Msa0246910-mRNA-1:exon:10266;Parent=Msa0246910-mRNA-1
chr2_2 exon 28703471 28704025 28703471 ID=Msa0246910-mRNA-1:exon:10267;Parent=Msa0246910-mRNA-1
chr2_2 five_prime_UTR 28700109 28700166 28700109 ID=Msa0246910-mRNA-1:five_prime_utr;Parent=Msa0246910-mRNA-1
chr2_2 CDS 28700167 28700313 28700167 ID=Msa0246910-mRNA-1:cds;Parent=Msa0246910-mRNA-1
chr2_2 CDS 28700462 28700613 28700462 ID=Msa0246910-mRNA-1:cds;Parent=Msa0246910-mRNA-1
chr2_2 CDS 28700912 28701156 28700912 ID=Msa0246910-mRNA-1:cds;Parent=Msa0246910-mRNA-1
chr2_2 CDS 28701354 28701479 28701354 ID=Msa0246910-mRNA-1:cds;Parent=Msa0246910-mRNA-1
chr2_2 CDS 28702055 28703017 28702055 ID=Msa0246910-mRNA-1:cds;Parent=Msa0246910-mRNA-1
chr2_2 CDS 28703471 28703601 28703471 ID=Msa0246910-mRNA-1:cds;Parent=Msa0246910-mRNA-1
chr2_2 three_prime_UTR 28703602 28704025 28703602 ID=Msa0246910-mRNA-1:three_prime_utr;Parent=Msa0246910-mRNA-1
Gene Sequence

>Msa0246910

ATGCATATCAAAATGATTGTCACTGTTCCATTATCAAAGGCTCTTTTTGCAGCTTTTCTCTTTGTCACCACCTTCCTCTTAACCTCCCCTGGATTTGTTTTGGCTACTTCACAACATGGAAGTGTTACAAGACACTACAAATTTGATATAAGGTTGGCAAAGGTTACGAGGCTGTGCCACACAAAAAGCATGGTTACCGTGAATGGGAAGTTCCCGGGTCCTCGAATTGTTGTACGAGAAGGGGACAGATTATTGGTTAAGGTTGTTAATCATGTTCCAAACAATATCAGCCTGCATTGGCATGGTGTGAGACAACTTCGTAGTGGTTGGTCGGATGGTCCATCATACATAACTCAATGCCCAATTCAAACAGGTCAGAGTTATGTGTACAACTTCACCATTGTTGGACAAAGAGGAACTCTCTTCTGGCATGCTCATTTCTCATGGTTAAGAGCTACTGTCTATGGACCTCTCATCCTTCTCCCAAGGCACAATGAATCTTATCCTTTTCAAAAACCCTACAAGGAAGTCCCCATTCTCTTTGGTGAGTGGTGGAATGCAGATCCTGAAGCTGTAATTGCACAAGCTCTGCAGACAGGGGGTGGCCCAAATGTTTCTGATGCCTACACCATTAATGGGTTTCCTGGACCTCTTTACAATTGCTCCAAAGATACATACAAATTGAAGGTGAAGCCAGGAAAAACATATCTATTACGTTTGATCAACGCTGCACTCAACGACGAACTCTTTTTCAGCATTGCTAATCACACATTAATCCTTGTCGAAGCAGATGCATCTTACATTAAACCATTCGAGTCCAATACCATCATACTTGGACCAGGTCAAACCACAAATGTTCTATTAAAAACTAAACCAAATTACCCAAATGCCACATTCTACATGCTAGCTAGACCATATTTCACAGGGCAGGGTACTTTCGACAATTCCACAGTAGCTGGAATTTTAGAATACACTAAACCAAATGATCAAACAAATAAAAATCTCCATATTCTAAAACCTGTGCTTCCAGCCATCAATGATACTAATTTTGTTGCTAATTTTAGTAACAAATTTCTTAGCTTAAATAGTCCAAAATACCCTGCAAATGTGCCTAAAACTATTGACAAAAACTTTTTCTTTACCGTTGGACTAGGAACTAGTCCATGCCCAAAAAACCAAACTTGTCAAGGACCAAATAATAGTTCAAATTCAAAATTTGCAGCTTCAATGAACAATGTATCATTTGCTTTACCTTCTATAGCACTTCTCCAACAACATTTCTTTGGAACTAATAATGGTTTTACAACGGATTTTCCGGTTGTACCGTTAAGGCCGTTTAACTATACAGGAACTCCTCCAAACAATACTTTGGTTAGTAATGGAACAAAGACAGTGGTGATACCATATAACACTAGAGTTCAGGTTATATTGCAGGATACAAGCATTTTGGGAGCTGAGAGTCATCCTTTGCATCTTCATGGATTTAATTTCTTTGTTGTTGGTCAAGGTTTTGGAAATTTTAATGCTAGTATTGACCCTGCAAGGTTTAATCTTGTTGATCCTGTTGAGAGGAATACTGTTGCTGTGCCTTCTGGTGGTTGGGTTGCGATACGATACCTTGCTGACAATCCAGGTGTTTGGCTGATGCATTGCCACTTTGATGTGCACCTTAGTTGGGGATTAAGGATGGCTTGGATTGTTGAGGATGGAAAGTTACCTAATCAAAAATTGCATCCTCCTCCTAAGGATCTCCCCAAATGTTGA
Protein sequence

>Msa0246910

MHIKMIVTVPLSKALFAAFLFVTTFLLTSPGFVLATSQHGSVTRHYKFDIRLAKVTRLCHTKSMVTVNGKFPGPRIVVREGDRLLVKVVNHVPNNISLHWHGVRQLRSGWSDGPSYITQCPIQTGQSYVYNFTIVGQRGTLFWHAHFSWLRATVYGPLILLPRHNESYPFQKPYKEVPILFGEWWNADPEAVIAQALQTGGGPNVSDAYTINGFPGPLYNCSKDTYKLKVKPGKTYLLRLINAALNDELFFSIANHTLILVEADASYIKPFESNTIILGPGQTTNVLLKTKPNYPNATFYMLARPYFTGQGTFDNSTVAGILEYTKPNDQTNKNLHILKPVLPAINDTNFVANFSNKFLSLNSPKYPANVPKTIDKNFFFTVGLGTSPCPKNQTCQGPNNSSNSKFAASMNNVSFALPSIALLQQHFFGTNNGFTTDFPVVPLRPFNYTGTPPNNTLVSNGTKTVVIPYNTRVQVILQDTSILGAESHPLHLHGFNFFVVGQGFGNFNASIDPARFNLVDPVERNTVAVPSGGWVAIRYLADNPGVWLMHCHFDVHLSWGLRMAWIVEDGKLPNQKLHPPPKDLPKC*