AlfalfaGEDB | Msa0317160

M. sativa cultivar zhongmu-4 / Msa0317160

KEGG: -
GO: GO:0003674 GO:0005488 GO:0048037 GO:0051536 GO:0051540
PFAM: PF04535.15

Nr:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa0317160	XP_003594276.1	99.005	201	2	0	1	201	1	201	1.09e-140	400

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Swissprot:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa0317160	sp\|A7R385\|CSPLC_VITVI	78.462	195	42	0	1	195	1	195	2.07e-105	304

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Trembl:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
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TFs/TRs:

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Gene ID	Type	Classification
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Protein Kinases:

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Gene ID	Type	Classification
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Network:

Co-expression Network

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Gene1	Gene2	correlation coefficient	p_value	FDR
Msa0032920	Msa0317160	-0.805810	1.115100e-49	-8.615850e-47
Msa0063890	Msa0317160	0.802634	5.110968e-49	-8.615850e-47
Msa0081520	Msa0317160	0.815563	8.668965e-52	-8.615850e-47
Msa0093850	Msa0317160	0.821876	3.197415e-53	-8.615850e-47
Msa0094580	Msa0317160	0.803960	2.716018e-49	-8.615850e-47
Msa0101440	Msa0317160	0.800273	1.557270e-48	-8.615850e-47
Msa0130240	Msa0317160	0.805564	1.256235e-49	-8.615850e-47
Msa0154440	Msa0317160	0.807874	4.084777e-50	-8.615850e-47
Msa0173890	Msa0317160	0.814592	1.424465e-51	-8.615850e-47
Msa0188890	Msa0317160	0.800596	1.337912e-48	-8.615850e-47

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PPI

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Gene1	Gene2	Type
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Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa0317160	MtrunA17_Chr2g0290281	98.895	181	2	0	21	201	1	181	6.79e-128	357
Msa0317160	MtrunA17_Chr4g0072591	29.379	177	120	2	24	195	1	177	4.42e-24	94.0
Msa0317160	MtrunA17_Chr3g0109681	32.317	164	101	3	28	191	42	195	3.02e-21	87.4
Msa0317160	MtrunA17_Chr5g0433491	30.909	165	104	4	28	191	34	189	1.01e-18	80.5
Msa0317160	MtrunA17_Chr4g0021111	30.822	146	85	3	29	171	6	138	3.23e-15	70.1
Msa0317160	MtrunA17_Chr7g0231191	26.316	171	116	4	28	198	19	179	6.91e-14	67.0
Msa0317160	MtrunA17_Chr8g0340351	29.814	161	104	3	26	177	33	193	1.96e-12	63.5
Msa0317160	MtrunA17_Chr7g0217691	26.744	172	118	3	20	189	20	185	4.78e-12	62.0
Msa0317160	MtrunA17_Chr7g0268681	27.895	190	122	6	9	191	5	186	1.08e-11	61.2
Msa0317160	MtrunA17_Chr4g0066711	31.410	156	104	3	32	187	29	181	1.80e-11	60.5

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Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa0317160	AT2G35760.1	68.718	195	60	1	1	195	1	194	1.91e-87	256
Msa0317160	AT4G16442.1	61.798	178	68	0	21	198	1	178	1.70e-79	235
Msa0317160	AT4G25830.1	35.294	170	104	3	27	195	3	167	3.03e-23	91.7
Msa0317160	AT1G17200.1	30.814	172	111	3	28	199	30	193	2.75e-22	90.1
Msa0317160	AT3G14380.1	29.240	171	103	3	23	188	15	172	5.60e-15	70.1
Msa0317160	AT4G15610.1	31.098	164	97	3	9	167	16	168	2.83e-14	68.6
Msa0317160	AT5G15290.1	28.000	150	103	2	23	170	20	166	9.64e-14	67.0
Msa0317160	AT4G15630.1	27.011	174	120	2	17	187	20	189	1.45e-11	61.2
Msa0317160	AT1G03700.1	29.710	138	87	2	29	166	7	134	2.13e-11	60.1
Msa0317160	AT4G03540.1	29.839	124	77	2	48	171	26	139	6.35e-11	58.9

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Find 60 sgRNAs with CRISPR-Local

Find 133 sgRNAs with CRISPR-GE

CRISPR-Local

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CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
AAAATGAGTTACTTGGGTGT+AGG	0.573937	2_4:+14720160	Msa0317160:exon
AAATTGCAAAACAACCAAAT+TGG	0.332368	2_4:-14722119	None:intergenic
AAGAAAACAGTGGATTTGAG+TGG	0.671545	2_4:-14722094	None:intergenic
AATAGGTCTGTTCTGACATC+TGG	0.349404	2_4:-14719905	None:intergenic
AATTACATGAATCATTAAAT+AGG	0.356990	2_4:-14719922	None:intergenic
ACATCTCAAACCTTGAATTA+AGG	0.337420	2_4:-14720635	None:intergenic
ACATGAAAGTGTTGGATAGG+AGG	0.523051	2_4:+14720221	Msa0317160:CDS
AGAAAATATAGCCCAAGCTA+AGG	0.484898	2_4:-14720698	None:intergenic
AGCTAAATTCACTGACATGA+AGG	0.513279	2_4:+14720357	Msa0317160:CDS
AGCTCCTTTATTGTATGTCT+AGG	0.530684	2_4:-14719829	None:intergenic

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CRISPR-GE

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sgRNA_sequence	Strand	Position	Region	GC_content
CCTAGAAAGAAAGTGATTAT+AGG	-	chr2_4:14719783-14719802	None:intergenic	30.0%
CCTATAATCACTTTCTTTCT+AGG	+	chr2_4:14719780-14719799	Msa0317160:five_prime_UTR	30.0%
TCTTCCTAGACATACAATAA+AGG	+	chr2_4:14719825-14719844	Msa0317160:five_prime_UTR	30.0%
AAAAAACAACACTCACAAAC+AGG	-	chr2_4:14720135-14720154	None:intergenic	30.0%
GACGTAAAAGTTAGTTATAC+TGG	+	chr2_4:14720437-14720456	Msa0317160:intron	30.0%
TAATAAACTTTACAGGTTCC+TGG	+	chr2_4:14720582-14720601	Msa0317160:intron	30.0%
ACATCTCAAACCTTGAATTA+AGG	-	chr2_4:14720638-14720657	None:intergenic	30.0%
TGTGTAGTGAGTATGATTAA+AGG	+	chr2_4:14720655-14720674	Msa0317160:CDS	30.0%
AGGTGAGTTTGAAAAAATCT+CGG	-	chr2_4:14720848-14720867	None:intergenic	30.0%
TGATATTGATGTTAGGACTA+AGG	+	chr2_4:14721148-14721167	Msa0317160:intron	30.0%

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Chromosome	Type	Strat	End	Strand	Name
chr2_4	gene	14719729	14722281	14719729	ID=Msa0317160;Name=Msa0317160
chr2_4	mRNA	14719729	14722281	14719729	ID=Msa0317160-mRNA-1;Parent=Msa0317160;Name=Msa0317160-mRNA-1;_AED=0.00;_eAED=0.00;_QI=434\|1\|1\|1\|1\|1\|3\|235\|201
chr2_4	exon	14719729	14720386	14719729	ID=Msa0317160-mRNA-1:exon:5524;Parent=Msa0317160-mRNA-1
chr2_4	exon	14720597	14720729	14720597	ID=Msa0317160-mRNA-1:exon:5525;Parent=Msa0317160-mRNA-1
chr2_4	exon	14721798	14722281	14721798	ID=Msa0317160-mRNA-1:exon:5526;Parent=Msa0317160-mRNA-1
chr2_4	five_prime_UTR	14719729	14720162	14719729	ID=Msa0317160-mRNA-1:five_prime_utr;Parent=Msa0317160-mRNA-1
chr2_4	CDS	14720163	14720386	14720163	ID=Msa0317160-mRNA-1:cds;Parent=Msa0317160-mRNA-1
chr2_4	CDS	14720597	14720729	14720597	ID=Msa0317160-mRNA-1:cds;Parent=Msa0317160-mRNA-1
chr2_4	CDS	14721798	14722046	14721798	ID=Msa0317160-mRNA-1:cds;Parent=Msa0317160-mRNA-1
chr2_4	three_prime_UTR	14722047	14722281	14722047	ID=Msa0317160-mRNA-1:three_prime_utr;Parent=Msa0317160-mRNA-1

Gene Sequence

>Msa0317160

ATGAGTTACTTGGGTGTAGGTGTTAGTCCTGGAACTGTTCCTGTTTATCATAGTACAAACATGAAAGTGTTGGATAGGAGGGTTAGAATAACTGAGTTGGTTTTGAGGTTTGTGAATCTTGGTTTAGGAGTTCTTGCTGTTGTTCTTATTGTGACTGATTCACAAGTTAGAGAGTTTTTCACCATTCAGAAGAAAGCTAAATTCACTGACATGAAGGCTTTAGTGTTCCTGGTTGTTGCTAATGCTATAACTGCTGGTTACTCCTTAATTCAAGGTTTGAGATGTGTAGTGAGTATGATTAAAGGAAGTGTGCTTTTCAACAAGCCCTTAGCTTGGGCTATATTTTCTGGTGATCAGGTAATGGCATATATAACAGTGGCAGCAGTGTCAGCTGCAGCACAATCTGCTGTGTTTGCAAAGATGGGCCAGGAACAATTGCAATGGATGAAGATATGCAATATGTATGGAAAATTCTGCAACCAAGTTGGAGAAGGGTTAGCTAGTGCATTTTTGGTTAGCCTTAGTATGGTGGTAGTATCATGTATTTCAGCTTTCAGTCTCTTCCGTTTGTATGGTGGTAGCAAAATTAAAAATGCGTATTGGTAA

Protein sequence

>Msa0317160

MSYLGVGVSPGTVPVYHSTNMKVLDRRVRITELVLRFVNLGLGVLAVVLIVTDSQVREFFTIQKKAKFTDMKALVFLVVANAITAGYSLIQGLRCVVSMIKGSVLFNKPLAWAIFSGDQVMAYITVAAVSAAAQSAVFAKMGQEQLQWMKICNMYGKFCNQVGEGLASAFLVSLSMVVVSCISAFSLFRLYGGSKIKNAYW*