AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa0188890


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0188890 MtrunA17_Chr2g0277971 97.959 147 3 0 1 147 1 147 3.84e-101 286
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0188890 AT1G60870.1 59.184 147 60 0 1 147 1 147 1.64e-33 115

Find 26 sgRNAs with CRISPR-Local

Find 25 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
AGCTGAGAAAGCAAGAAATA+TGG 0.282094 2_1:+4099669 Msa0188890:CDS
GCTTGGAAAGGAATTTCTTC+TGG 0.317736 2_1:+4099778 Msa0188890:CDS
AGCAGCTGCCCATGCTTTGT+AGG 0.349595 2_1:-4099485 None:intergenic
AAGAAATATGGGAAACTTGA+TGG 0.389367 2_1:+4099681 Msa0188890:CDS
GTGCCATGGATGAATTTAGA+AGG 0.394252 2_1:+4099590 Msa0188890:CDS
GGAATATCTCGACTCGGCTA+TGG 0.422650 2_1:+4099564 Msa0188890:CDS
GAGGATTTCAAGAGATGAAG+TGG 0.462068 2_1:+4099630 Msa0188890:CDS
GCTGAGAAAGCAAGAAATAT+GGG 0.463308 2_1:+4099670 Msa0188890:CDS
ATAAGATCTTCGATCGTCGA+TGG 0.506443 2_1:-4099442 None:intergenic
GAAAGGAATTTCTTCTGGCA+AGG 0.513277 2_1:+4099783 Msa0188890:CDS
AGATAGAATCCTACAAAGCA+TGG 0.513381 2_1:+4099476 Msa0188890:CDS
AAAGGAAGGTGAACTGAGAA+AGG 0.516993 2_1:-4099387 None:intergenic
CTGCTTCTATGAAATCTGCT+TGG 0.529588 2_1:+4099761 Msa0188890:CDS
GGTTTGAAGAAGAACTTGAG+AGG 0.547329 2_1:+4099611 Msa0188890:CDS
AAACCTTCTAAATTCATCCA+TGG 0.552800 2_1:-4099593 None:intergenic
TAAAGATTGATCTGAAAGGA+AGG 0.570141 2_1:-4099401 None:intergenic
AAAGTATATATGTATCAAGA+TGG 0.576287 2_1:-4099811 None:intergenic
CTCGGCTATGGAAAGTGCCA+TGG 0.577831 2_1:+4099576 Msa0188890:CDS
TCTATGAAATCTGCTTGGAA+AGG 0.578134 2_1:+4099766 Msa0188890:CDS
CATGTAAAGATTGATCTGAA+AGG 0.594535 2_1:-4099405 None:intergenic
AGCCGAGTCGAGATATTCCT+CGG 0.604035 2_1:-4099560 None:intergenic
TGAACTTGAGCAAGAAAGAG+AGG 0.624850 2_1:+4099507 Msa0188890:CDS
AGCCGAGGAATATCTCGACT+CGG 0.631008 2_1:+4099558 Msa0188890:CDS
GATAGAATCCTACAAAGCAT+GGG 0.638623 2_1:+4099477 Msa0188890:CDS
AGATGCAATGCAAGAAGCCG+AGG 0.640190 2_1:+4099543 Msa0188890:CDS
ACTTGAGCAAGAAAGAGAGG+TGG 0.641638 2_1:+4099510 Msa0188890:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
TAAAGATTGATCTGAAAGGA+AGG - chr2_1:4099404-4099423 None:intergenic 30.0%
CATGTAAAGATTGATCTGAA+AGG - chr2_1:4099408-4099427 None:intergenic 30.0%
AAACCTTCTAAATTCATCCA+TGG - chr2_1:4099596-4099615 None:intergenic 30.0%
AAGAAATATGGGAAACTTGA+TGG + chr2_1:4099681-4099700 Msa0188890:CDS 30.0%
AGATAGAATCCTACAAAGCA+TGG + chr2_1:4099476-4099495 Msa0188890:CDS 35.0%
GATAGAATCCTACAAAGCAT+GGG + chr2_1:4099477-4099496 Msa0188890:CDS 35.0%
AGCTGAGAAAGCAAGAAATA+TGG + chr2_1:4099669-4099688 Msa0188890:CDS 35.0%
GCTGAGAAAGCAAGAAATAT+GGG + chr2_1:4099670-4099689 Msa0188890:CDS 35.0%
TCTATGAAATCTGCTTGGAA+AGG + chr2_1:4099766-4099785 Msa0188890:CDS 35.0%
AAAGGAAGGTGAACTGAGAA+AGG - chr2_1:4099390-4099409 None:intergenic 40.0%
ATAAGATCTTCGATCGTCGA+TGG - chr2_1:4099445-4099464 None:intergenic 40.0%
TGAACTTGAGCAAGAAAGAG+AGG + chr2_1:4099507-4099526 Msa0188890:CDS 40.0%
GTGCCATGGATGAATTTAGA+AGG + chr2_1:4099590-4099609 Msa0188890:CDS 40.0%
GGTTTGAAGAAGAACTTGAG+AGG + chr2_1:4099611-4099630 Msa0188890:CDS 40.0%
GAGGATTTCAAGAGATGAAG+TGG + chr2_1:4099630-4099649 Msa0188890:CDS 40.0%
CTGCTTCTATGAAATCTGCT+TGG + chr2_1:4099761-4099780 Msa0188890:CDS 40.0%
GCTTGGAAAGGAATTTCTTC+TGG + chr2_1:4099778-4099797 Msa0188890:CDS 40.0%
GAAAGGAATTTCTTCTGGCA+AGG + chr2_1:4099783-4099802 Msa0188890:CDS 40.0%
ACTTGAGCAAGAAAGAGAGG+TGG + chr2_1:4099510-4099529 Msa0188890:CDS 45.0%
AGATGCAATGCAAGAAGCCG+AGG + chr2_1:4099543-4099562 Msa0188890:CDS 50.0%
AGCCGAGGAATATCTCGACT+CGG + chr2_1:4099558-4099577 Msa0188890:CDS 50.0%
AGCCGAGTCGAGATATTCCT+CGG - chr2_1:4099563-4099582 None:intergenic 50.0%
!! GGAATATCTCGACTCGGCTA+TGG + chr2_1:4099564-4099583 Msa0188890:CDS 50.0%
AGCAGCTGCCCATGCTTTGT+AGG - chr2_1:4099488-4099507 None:intergenic 55.0%
!! CTCGGCTATGGAAAGTGCCA+TGG + chr2_1:4099576-4099595 Msa0188890:CDS 55.0%
Chromosome Type Strat End Strand Name
chr2_1 gene 4099376 4099819 4099376 ID=Msa0188890;Name=Msa0188890
chr2_1 mRNA 4099376 4099819 4099376 ID=Msa0188890-mRNA-1;Parent=Msa0188890;Name=Msa0188890-mRNA-1;_AED=0.00;_eAED=0.00;_QI=0|-1|0|1|-1|1|1|0|147
chr2_1 exon 4099376 4099819 4099376 ID=Msa0188890-mRNA-1:exon:1825;Parent=Msa0188890-mRNA-1
chr2_1 CDS 4099376 4099819 4099376 ID=Msa0188890-mRNA-1:cds;Parent=Msa0188890-mRNA-1
Gene Sequence

>Msa0188890

ATGGAAGCTCTCCTTTCTCAGTTCACCTTCCTTTCAGATCAATCTTTACATGACAAGAGCTTCGATCCATCGACGATCGAAGATCTTATGAAACTATTTGAGATAGAATCCTACAAAGCATGGGCAGCTGCTGAACTTGAGCAAGAAAGAGAGGTGGAAGAAGCAGAAGATGCAATGCAAGAAGCCGAGGAATATCTCGACTCGGCTATGGAAAGTGCCATGGATGAATTTAGAAGGTTTGAAGAAGAACTTGAGAGGATTTCAAGAGATGAAGTGGAGAGTTTAGTTCAAACAGCTGAGAAAGCAAGAAATATGGGAAACTTGATGGAAAAAACAGCTTCTATTGCTTCTAAGAAATATATTGAAGCTGCACTTAATTCAGCTACTGCTTCTATGAAATCTGCTTGGAAAGGAATTTCTTCTGGCAAGGTTCATCCATCTTGA

Protein sequence

>Msa0188890

MEALLSQFTFLSDQSLHDKSFDPSTIEDLMKLFEIESYKAWAAAELEQEREVEEAEDAMQEAEEYLDSAMESAMDEFRRFEEELERISRDEVESLVQTAEKARNMGNLMEKTASIASKKYIEAALNSATASMKSAWKGISSGKVHPS*