AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa0320720

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score

Find 60 sgRNAs with CRISPR-Local

Find 192 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
CATTCCAACTTTGTACTTTA+AGG 0.047552 2_4:+21425567 None:intergenic
GATTCTGAGTTACAACTAAT+TGG 0.169825 2_4:-21425018 Msa0320720:three_prime_UTR
GTCTCCTCTGTGAGCACTTA+TGG 0.248211 2_4:-21427350 Msa0320720:CDS
ATTCAATCGGTCAATTAATA+AGG 0.287292 2_4:+21424931 None:intergenic
TAAAATAAATGCTTAAATAA+TGG 0.309201 2_4:+21425321 None:intergenic
ATTCCAACTTTGTACTTTAA+GGG 0.318235 2_4:+21425568 None:intergenic
TTGGCCTACTGATATGGATA+TGG 0.323907 2_4:-21427793 Msa0320720:CDS
ATTCTGAGTTACAACTAATT+GGG 0.330830 2_4:-21425017 Msa0320720:three_prime_UTR
TAGGGACTCGAATTCTTAAT+AGG 0.375298 2_4:-21425419 Msa0320720:three_prime_UTR
TATGAAAAGTTGAATATTGT+TGG 0.378727 2_4:-21425473 Msa0320720:three_prime_UTR
CCTTGTTCTTTGTGTGAGCT+TGG 0.386697 2_4:+21425655 None:intergenic
CACACCATCCCTTGTATAGT+TGG 0.388307 2_4:+21425389 None:intergenic
GTGTAAAAGAGGTCCATATT+AGG 0.394434 2_4:+21424990 None:intergenic
ACTGCTTCTAATTACATGAT+AGG 0.394642 2_4:+21425091 None:intergenic
AATGCTAAATTAAACTATAT+AGG 0.416900 2_4:-21425527 Msa0320720:three_prime_UTR
AGGACCGTGGGTGGAGGAAA+TGG 0.432827 2_4:+21424951 None:intergenic
GTTGAATATTGTTGGAACAA+TGG 0.446379 2_4:-21425465 Msa0320720:three_prime_UTR
TGAGCACTTATGGTCGTAAC+TGG 0.451215 2_4:-21427340 Msa0320720:CDS
GATGTCGTACCAAGCAGAAA+TGG 0.452468 2_4:-21425190 Msa0320720:three_prime_UTR
TGCTATGTAAGGAAAGACTA+TGG 0.456105 2_4:+21427848 None:intergenic
TTAAAGTACAAAGTTGGAAT+GGG 0.460423 2_4:-21425565 Msa0320720:three_prime_UTR
TGTACTTTAAGGGCTTCAAT+TGG 0.463577 2_4:+21425578 None:intergenic
CTGGTTGGACACAACATACT+TGG 0.464582 2_4:+21425617 None:intergenic
CTTAAAGTACAAAGTTGGAA+TGG 0.466864 2_4:-21425566 Msa0320720:three_prime_UTR
AAGCCCTTAAAGTACAAAGT+TGG 0.470446 2_4:-21425571 Msa0320720:three_prime_UTR
ACACCATCCCTTGTATAGTT+GGG 0.474764 2_4:+21425390 None:intergenic
ATCCATATCAGTAGGCCAAA+AGG 0.488360 2_4:+21427797 None:intergenic
GATCAAGATAGCATAGGGAT+AGG 0.491298 2_4:-21425438 Msa0320720:three_prime_UTR
TGGAATGTAAACCCTTCCTC+TGG 0.495186 2_4:+21425598 None:intergenic
CTTGTCACTGTGTTAGACTC+AGG 0.497067 2_4:-21427286 Msa0320720:intron
GACTCGAATTCTTAATAGGC+AGG 0.502520 2_4:-21425415 Msa0320720:three_prime_UTR
TGATCTGAACAGTGTAAAAG+AGG 0.509203 2_4:+21424979 None:intergenic
TTTCCACGACTGAGTGTGAC+TGG 0.512586 2_4:+21425215 None:intergenic
GGCAGGAACCCAACTATACA+AGG 0.534735 2_4:-21425398 Msa0320720:three_prime_UTR
TTAATAAGGACCGTGGGTGG+AGG 0.546474 2_4:+21424945 None:intergenic
ATCAAGATAGCATAGGGATA+GGG 0.551154 2_4:-21425437 Msa0320720:three_prime_UTR
TTCTGAGTTACAACTAATTG+GGG 0.555243 2_4:-21425016 Msa0320720:three_prime_UTR
CGGAGGCTAACGCAGTGACA+AGG 0.558881 2_4:+21427375 None:intergenic
TGTTGTGTCCAACCAGAGGA+AGG 0.568465 2_4:-21425610 Msa0320720:CDS
ATGTAAACCCTTCCTCTGGT+TGG 0.582925 2_4:+21425602 None:intergenic
TGACAAGGTACAGAGATCTC+AGG 0.588071 2_4:+21427302 None:intergenic
AGCAGCAAATCTACATACAT+AGG 0.596540 2_4:+21425249 None:intergenic
CCAAGCTCACACAAAGAACA+AGG 0.605053 2_4:-21425655 Msa0320720:CDS
CAATTAATAAGGACCGTGGG+TGG 0.605970 2_4:+21424942 None:intergenic
GTTGTGTCCAACCAGAGGAA+GGG 0.610598 2_4:-21425609 Msa0320720:CDS
GAACCCAACTATACAAGGGA+TGG 0.613369 2_4:-21425393 Msa0320720:three_prime_UTR
CGGTCAATTAATAAGGACCG+TGG 0.615141 2_4:+21424938 None:intergenic
AGAAGTTGTATCGATGACAT+AGG 0.615984 2_4:-21425280 Msa0320720:three_prime_UTR
GATATGGATATGGTAGACTC+AGG 0.623990 2_4:-21427783 Msa0320720:intron
TCTACCATATCCATATCAGT+AGG 0.628700 2_4:+21427789 None:intergenic
ATACCAGTCACACTCAGTCG+TGG 0.635423 2_4:-21425218 Msa0320720:three_prime_UTR
GGAGGCTAACGCAGTGACAA+GGG 0.635819 2_4:+21427376 None:intergenic
CTGAGTCTAACACAGTGACA+AGG 0.661348 2_4:+21427287 None:intergenic
GGTCAATTAATAAGGACCGT+GGG 0.678476 2_4:+21424939 None:intergenic
AGTATGTTGTGTCCAACCAG+AGG 0.686559 2_4:-21425614 Msa0320720:CDS
AGTGCTCACAGAGGAGACGA+CGG 0.708102 2_4:+21427355 None:intergenic
GCAGGAACCCAACTATACAA+GGG 0.709629 2_4:-21425397 Msa0320720:three_prime_UTR
GCTCACAGAGGAGACGACGG+AGG 0.740555 2_4:+21427358 None:intergenic
TCTGAGTTACAACTAATTGG+GGG 0.747189 2_4:-21425015 Msa0320720:three_prime_UTR
ACGACCATAAGTGCTCACAG+AGG 0.812409 2_4:+21427346 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!!! ATTTTTTATTTTTTTATTTT+TGG + chr2_4:21426159-21426178 None:intergenic 0.0%
!! AAACAAAAAAAAAAAACTTA+TGG + chr2_4:21425378-21425397 None:intergenic 10.0%
!!! CTTAAAATTTTAAAACATAA+AGG + chr2_4:21425951-21425970 None:intergenic 10.0%
!!! TATGTTTTAAAATTTTAAGT+TGG - chr2_4:21425952-21425971 Msa0320720:intron 10.0%
!! AAAACTTAAAATAAACAAAA+GGG - chr2_4:21426301-21426320 Msa0320720:intron 10.0%
!! AAAAAAAAACTCAAATAATT+AGG + chr2_4:21426641-21426660 None:intergenic 10.0%
!! ATGATAATTTAAAAGAAATA+TGG + chr2_4:21427084-21427103 None:intergenic 10.0%
!!! TATTTCTTTTAAATTATCAT+AGG - chr2_4:21427084-21427103 Msa0320720:intron 10.0%
!! TAAAATAAATGCTTAAATAA+TGG + chr2_4:21427511-21427530 None:intergenic 10.0%
!! TTTAAATATCATTAGTTCAT+AGG - chr2_4:21425069-21425088 Msa0320720:three_prime_UTR 15.0%
!!! CTTTTATTTTATTTCTTTAC+CGG - chr2_4:21425329-21425348 Msa0320720:three_prime_UTR 15.0%
!!! GTTTATTTTAGTTTTTAAGT+AGG - chr2_4:21425924-21425943 Msa0320720:intron 15.0%
!!! TTAAAGCTTTTAAAACATAA+GGG - chr2_4:21426270-21426289 Msa0320720:intron 15.0%
!!! TTTATTTTAAGTTTTGAGTT+GGG + chr2_4:21426296-21426315 None:intergenic 15.0%
!! CAAAACTTAAAATAAACAAA+AGG - chr2_4:21426300-21426319 Msa0320720:intron 15.0%
!!! TATATTTATGTTTTAAAGCT+AGG - chr2_4:21426958-21426977 Msa0320720:intron 15.0%
!! AATGCTAAATTAAACTATAT+AGG - chr2_4:21427302-21427321 Msa0320720:CDS 15.0%
!!! TTCTTGTTATTTTTACCTTT+TGG - chr2_4:21425017-21425036 Msa0320720:three_prime_UTR 20.0%
!!! GTGTTATTTTGATATAGATA+TGG - chr2_4:21425091-21425110 Msa0320720:three_prime_UTR 20.0%
!! CGGTAAAGAAATAAAATAAA+AGG + chr2_4:21425331-21425350 None:intergenic 20.0%
!!! TTCAAGACTATTTAACTTTA+AGG - chr2_4:21425691-21425710 Msa0320720:intron 20.0%
!!! GTAAAACATTGGTTTAATAA+AGG + chr2_4:21425843-21425862 None:intergenic 20.0%
!! AAAAACTAAAATAAACGTGA+GGG + chr2_4:21425920-21425939 None:intergenic 20.0%
!! TAAAAACTAAAATAAACGTG+AGG + chr2_4:21425921-21425940 None:intergenic 20.0%
!! TTACTAAACTCAATATTATG+TGG + chr2_4:21426048-21426067 None:intergenic 20.0%
!!! TTTATTTTTGGAATGAAAGA+GGG + chr2_4:21426147-21426166 None:intergenic 20.0%
!!! TTTTATTTTTGGAATGAAAG+AGG + chr2_4:21426148-21426167 None:intergenic 20.0%
!!! TTCCAATATGATTAACATTT+TGG - chr2_4:21426241-21426260 Msa0320720:intron 20.0%
!!! CTTAAAGCTTTTAAAACATA+AGG - chr2_4:21426269-21426288 Msa0320720:intron 20.0%
!!! GTTTATTTTAAGTTTTGAGT+TGG + chr2_4:21426297-21426316 None:intergenic 20.0%
!!! ATGTTATAAGCACTTTTAAT+TGG - chr2_4:21426446-21426465 Msa0320720:intron 20.0%
!!! CACTTTTAATTGGATAAATA+AGG - chr2_4:21426456-21426475 Msa0320720:intron 20.0%
!! ATAAGTTCTATAGTAGTATA+TGG - chr2_4:21426825-21426844 Msa0320720:intron 20.0%
!! TATGAAAAGTTGAATATTGT+TGG - chr2_4:21427356-21427375 Msa0320720:CDS 20.0%
!! GACTTCTATTAACAAAAAAA+AGG + chr2_4:21427701-21427720 None:intergenic 20.0%
! TATCATATATTCTTTGCTGA+AGG - chr2_4:21425130-21425149 Msa0320720:three_prime_UTR 25.0%
! TAAGTTCTATAGTAGTATCA+TGG - chr2_4:21425196-21425215 Msa0320720:three_prime_UTR 25.0%
! ATAAGTGTCTGCAAATAAAA+GGG - chr2_4:21425235-21425254 Msa0320720:three_prime_UTR 25.0%
!!! TGTTATAATGAGTGTTTTGA+GGG + chr2_4:21425282-21425301 None:intergenic 25.0%
! AATAAAATAAAAGGCTGATG+TGG + chr2_4:21425322-21425341 None:intergenic 25.0%
!!! ATTATTTTGATTAACTGCCA+TGG - chr2_4:21425589-21425608 Msa0320720:exon 25.0%
!!! TTATTTTGATTAACTGCCAT+GGG - chr2_4:21425590-21425609 Msa0320720:exon 25.0%
!!! TAAGCTTGTAGAGTATTTTA+TGG - chr2_4:21425769-21425788 Msa0320720:intron 25.0%
!!! AAGCTTGTAGAGTATTTTAT+GGG - chr2_4:21425770-21425789 Msa0320720:intron 25.0%
! CAAAATAGGATGTAAAACAT+TGG + chr2_4:21425854-21425873 None:intergenic 25.0%
!!! TATGCACACGTATAATTTTT+TGG - chr2_4:21425876-21425895 Msa0320720:intron 25.0%
! AAAACTAAAATAAACGTGAG+GGG + chr2_4:21425919-21425938 None:intergenic 25.0%
!!! TTGTTTTAAGAGTTTCAAGT+AGG - chr2_4:21425981-21426000 Msa0320720:intron 25.0%
!! TAAATTCCAACACTCATTTT+TGG - chr2_4:21426114-21426133 Msa0320720:intron 25.0%
!!! AAATTCCAACACTCATTTTT+GGG - chr2_4:21426115-21426134 Msa0320720:intron 25.0%
! GTCCAAAATGTTAATCATAT+TGG + chr2_4:21426246-21426265 None:intergenic 25.0%
!! GCTATGTTTCATTTGATTTA+AGG - chr2_4:21426501-21426520 Msa0320720:intron 25.0%
!! CATTTGATTTAAGGCATATA+TGG - chr2_4:21426510-21426529 Msa0320720:intron 25.0%
! GTACAACAATTCAAATAACT+TGG + chr2_4:21426561-21426580 None:intergenic 25.0%
!! ATCAGCATGTATTCTTTTTA+TGG - chr2_4:21426614-21426633 Msa0320720:intron 25.0%
! ATGATGATATACTTTCTTTG+AGG - chr2_4:21426791-21426810 Msa0320720:intron 25.0%
! ACAAGTGTCTTCAAATAAAA+GGG - chr2_4:21426864-21426883 Msa0320720:intron 25.0%
! GAAACAAATCTATTAGCAAT+TGG - chr2_4:21426934-21426953 Msa0320720:intron 25.0%
! ACAACCATTTACATTCAAAA+GGG + chr2_4:21427051-21427070 None:intergenic 25.0%
! ATTCCAACTTTGTACTTTAA+GGG + chr2_4:21427264-21427283 None:intergenic 25.0%
! TTAAAGTACAAAGTTGGAAT+GGG - chr2_4:21427264-21427283 Msa0320720:intron 25.0%
!!! TGTTGTTTTACTTACATGAA+TGG - chr2_4:21427331-21427350 Msa0320720:CDS 25.0%
! ATTCTGAGTTACAACTAATT+GGG - chr2_4:21427812-21427831 Msa0320720:CDS 25.0%
AAAAAGTCAAGTGCTATGTA+AGG + chr2_4:21424995-21425014 None:intergenic 30.0%
GATATAGATATGGTATACTC+AGG - chr2_4:21425101-21425120 Msa0320720:three_prime_UTR 30.0%
ATGATGATATACTTTCCTTG+AGG - chr2_4:21425165-21425184 Msa0320720:three_prime_UTR 30.0%
GATAAGTGTCTGCAAATAAA+AGG - chr2_4:21425234-21425253 Msa0320720:three_prime_UTR 30.0%
! GTGTTATAATGAGTGTTTTG+AGG + chr2_4:21425283-21425302 None:intergenic 30.0%
! TTTATTTCTTTACCGGCTAA+CGG - chr2_4:21425336-21425355 Msa0320720:three_prime_UTR 30.0%
GATGTAGTCCTATAAAATAC+AGG + chr2_4:21425637-21425656 None:intergenic 30.0%
ACATATTAGATAGAGGTTGA+AGG + chr2_4:21425818-21425837 None:intergenic 30.0%
AAAGGTCACATATTAGATAG+AGG + chr2_4:21425825-21425844 None:intergenic 30.0%
TACGTGTGCATAAACAAAAT+AGG + chr2_4:21425868-21425887 None:intergenic 30.0%
!!! TTTTGGCTTAATTGCATGTT+TGG - chr2_4:21425893-21425912 Msa0320720:intron 30.0%
! TTCCAAAATGAGTAACGTTT+TGG + chr2_4:21426008-21426027 None:intergenic 30.0%
CTAAACTCAATATTATGTGG+CGG + chr2_4:21426045-21426064 None:intergenic 30.0%
AATAGGCTTAATTGCATGTT+TGG + chr2_4:21426328-21426347 None:intergenic 30.0%
CCTTTACAAGGTAAAAGAAT+AGG + chr2_4:21426345-21426364 None:intergenic 30.0%
! CCTATTCTTTTACCTTGTAA+AGG - chr2_4:21426342-21426361 Msa0320720:intron 30.0%
CAATTCAAATAACTTGGTTG+AGG + chr2_4:21426555-21426574 None:intergenic 30.0%
AATCAAACAAACAACAAGCT+TGG + chr2_4:21426731-21426750 None:intergenic 30.0%
!! TGATATACTTTCTTTGAGGT+TGG - chr2_4:21426795-21426814 Msa0320720:intron 30.0%
GACAAGTGTCTTCAAATAAA+AGG - chr2_4:21426863-21426882 Msa0320720:intron 30.0%
AAGGGATATATTTACCTCTA+AGG - chr2_4:21426882-21426901 Msa0320720:intron 30.0%
GACAACCATTTACATTCAAA+AGG + chr2_4:21427052-21427071 None:intergenic 30.0%
CATTCCAACTTTGTACTTTA+AGG + chr2_4:21427265-21427284 None:intergenic 30.0%
CTTAAAGTACAAAGTTGGAA+TGG - chr2_4:21427263-21427282 Msa0320720:intron 30.0%
GTTGAATATTGTTGGAACAA+TGG - chr2_4:21427364-21427383 Msa0320720:CDS 30.0%
!! GTTTTGATCAAGATAGCATA+GGG - chr2_4:21427386-21427405 Msa0320720:intron 30.0%
!!! TTTTGAGTTGCTTAAAGAAC+TGG - chr2_4:21427665-21427684 Msa0320720:intron 30.0%
ACTGCTTCTAATTACATGAT+AGG + chr2_4:21427741-21427760 None:intergenic 30.0%
GATTCTGAGTTACAACTAAT+TGG - chr2_4:21427811-21427830 Msa0320720:CDS 30.0%
TTCTGAGTTACAACTAATTG+GGG - chr2_4:21427813-21427832 Msa0320720:CDS 30.0%
TGCTATGTAAGGAAAGACTA+TGG + chr2_4:21424984-21425003 None:intergenic 35.0%
TCTACCATATCCATATCAGT+AGG + chr2_4:21425043-21425062 None:intergenic 35.0%
TATTCTTTGCTGAAGGAGTA+TGG - chr2_4:21425137-21425156 Msa0320720:three_prime_UTR 35.0%
GAACTTATCATTCAACCTCA+AGG + chr2_4:21425183-21425202 None:intergenic 35.0%
AAAACTTATGGCTCAGAACT+TGG + chr2_4:21425366-21425385 None:intergenic 35.0%
! GTCCATGTCCTGTATTTTAT+AGG - chr2_4:21425626-21425645 Msa0320720:CDS 35.0%
GTCCTATAAAATACAGGACA+TGG + chr2_4:21425631-21425650 None:intergenic 35.0%
! ATATTTTCTCGTGCACAAAC+TGG - chr2_4:21425739-21425758 Msa0320720:intron 35.0%
! GTCCAAAACGTTACTCATTT+TGG - chr2_4:21426003-21426022 Msa0320720:intron 35.0%
GGAAAAATCTCTCCTTTACA+AGG + chr2_4:21426357-21426376 None:intergenic 35.0%
TTCCAAGTATCTCACCTATA+AGG - chr2_4:21426373-21426392 Msa0320720:intron 35.0%
GACTCTTTACTGAAAGAGTA+TGG - chr2_4:21426751-21426770 Msa0320720:intron 35.0%
! GGAAGCACATGAACTATATT+GGG + chr2_4:21427011-21427030 None:intergenic 35.0%
! TGGTCCCTTTTGAATGTAAA+TGG - chr2_4:21427044-21427063 Msa0320720:intron 35.0%
! TGTACTTTAAGGGCTTCAAT+TGG + chr2_4:21427254-21427273 None:intergenic 35.0%
AAGCCCTTAAAGTACAAAGT+TGG - chr2_4:21427258-21427277 Msa0320720:intron 35.0%
!! GGTTTTGATCAAGATAGCAT+AGG - chr2_4:21427385-21427404 Msa0320720:CDS 35.0%
ATCAAGATAGCATAGGGATA+GGG - chr2_4:21427392-21427411 Msa0320720:intron 35.0%
TAGGGACTCGAATTCTTAAT+AGG - chr2_4:21427410-21427429 Msa0320720:intron 35.0%
! AGAAGTTGTATCGATGACAT+AGG - chr2_4:21427549-21427568 Msa0320720:intron 35.0%
AGCAGCAAATCTACATACAT+AGG + chr2_4:21427583-21427602 None:intergenic 35.0%
TCAAAAAAGCCATTTCTGCT+TGG + chr2_4:21427651-21427670 None:intergenic 35.0%
TCTGAGTTACAACTAATTGG+GGG - chr2_4:21427814-21427833 Msa0320720:CDS 35.0%
GTGTAAAAGAGGTCCATATT+AGG + chr2_4:21427842-21427861 None:intergenic 35.0%
TGATCTGAACAGTGTAAAAG+AGG + chr2_4:21427853-21427872 None:intergenic 35.0%
! TACCTTTTGGCCTACTGATA+TGG - chr2_4:21425030-21425049 Msa0320720:three_prime_UTR 40.0%
ATCCATATCAGTAGGCCAAA+AGG + chr2_4:21425035-21425054 None:intergenic 40.0%
TTGGCCTACTGATATGGATA+TGG - chr2_4:21425036-21425055 Msa0320720:three_prime_UTR 40.0%
GATATGGATATGGTAGACTC+AGG - chr2_4:21425046-21425065 Msa0320720:three_prime_UTR 40.0%
GTCTGCAAATAAAAGGGATG+AGG - chr2_4:21425241-21425260 Msa0320720:three_prime_UTR 40.0%
TAAAAGGGATGAGGAATGCT+AGG - chr2_4:21425250-21425269 Msa0320720:three_prime_UTR 40.0%
AAAAGGGATGAGGAATGCTA+GGG - chr2_4:21425251-21425270 Msa0320720:three_prime_UTR 40.0%
CACGAGAAAATATGCGAAAG+TGG + chr2_4:21425732-21425751 None:intergenic 40.0%
! CTCAATATTATGTGGCGGAA+GGG + chr2_4:21426040-21426059 None:intergenic 40.0%
! ACTCAATATTATGTGGCGGA+AGG + chr2_4:21426041-21426060 None:intergenic 40.0%
TGAAGCCCAAAAATGAGTGT+TGG + chr2_4:21426123-21426142 None:intergenic 40.0%
TGCCTTATAGGTGAGATACT+TGG + chr2_4:21426378-21426397 None:intergenic 40.0%
! CTTTTGTATGTGCCCTACTA+TGG - chr2_4:21426585-21426604 Msa0320720:intron 40.0%
! GCTGATTTAGTGCCATAGTA+GGG + chr2_4:21426600-21426619 None:intergenic 40.0%
!! TGCTGATTTAGTGCCATAGT+AGG + chr2_4:21426601-21426620 None:intergenic 40.0%
! GTAATGTTGCTTCACCTTAG+AGG + chr2_4:21426899-21426918 None:intergenic 40.0%
AGGTGAAGCAACATTACGTA+TGG - chr2_4:21426902-21426921 Msa0320720:intron 40.0%
AGCACATGAACTATATTGGG+CGG + chr2_4:21427008-21427027 None:intergenic 40.0%
! CGGAAGCACATGAACTATAT+TGG + chr2_4:21427012-21427031 None:intergenic 40.0%
TGCTTCCGAAAATTGCTATG+TGG - chr2_4:21427024-21427043 Msa0320720:intron 40.0%
! AGGGACCACATAGCAATTTT+CGG + chr2_4:21427032-21427051 None:intergenic 40.0%
GATCAAGATAGCATAGGGAT+AGG - chr2_4:21427391-21427410 Msa0320720:intron 40.0%
GACTCGAATTCTTAATAGGC+AGG - chr2_4:21427414-21427433 Msa0320720:intron 40.0%
ACACCATCCCTTGTATAGTT+GGG + chr2_4:21427442-21427461 None:intergenic 40.0%
GGTCAATTAATAAGGACCGT+GGG + chr2_4:21427893-21427912 None:intergenic 40.0%
TGATGTGGCTTCACATGAGA+GGG + chr2_4:21425307-21425326 None:intergenic 45.0%
GAACTTGGTTAACCGTTAGC+CGG + chr2_4:21425351-21425370 None:intergenic 45.0%
AGCCATAGAGGTTGTGTTGA+GGG + chr2_4:21425421-21425440 None:intergenic 45.0%
AAGCCATAGAGGTTGTGTTG+AGG + chr2_4:21425422-21425441 None:intergenic 45.0%
ACAGATCTGCAAAGCCATAG+AGG + chr2_4:21425433-21425452 None:intergenic 45.0%
TGAGCACTTATGGTCGTAAC+TGG - chr2_4:21425489-21425508 Msa0320720:three_prime_UTR 45.0%
TGACAAGGTACAGAGATCTC+AGG + chr2_4:21425530-21425549 None:intergenic 45.0%
CTGAGTCTAACACAGTGACA+AGG + chr2_4:21425545-21425564 None:intergenic 45.0%
CTTGTCACTGTGTTAGACTC+AGG - chr2_4:21425543-21425562 Msa0320720:three_prime_UTR 45.0%
GACAACATAGTACACACCCA+TGG + chr2_4:21425609-21425628 None:intergenic 45.0%
TGGGCTGAGAATATGAGTGT+TGG + chr2_4:21426183-21426202 None:intergenic 45.0%
TCTCAGCCCAAACCTCTAAA+CGG - chr2_4:21426193-21426212 Msa0320720:intron 45.0%
ACTTGGCCGTTTAGAGGTTT+GGG + chr2_4:21426202-21426221 None:intergenic 45.0%
! TAAACGGCCAAGTGATGGAA+GGG - chr2_4:21426209-21426228 Msa0320720:intron 45.0%
AGTTAGTCCCTTCCATCACT+TGG + chr2_4:21426219-21426238 None:intergenic 45.0%
GAAGCTTCTTGCTGCCTTAT+AGG + chr2_4:21426390-21426409 None:intergenic 45.0%
CCTTGTTCTTTGTGTGAGCT+TGG + chr2_4:21427177-21427196 None:intergenic 45.0%
CCAAGCTCACACAAAGAACA+AGG - chr2_4:21427174-21427193 Msa0320720:intron 45.0%
CTGGTTGGACACAACATACT+TGG + chr2_4:21427215-21427234 None:intergenic 45.0%
AGTATGTTGTGTCCAACCAG+AGG - chr2_4:21427215-21427234 Msa0320720:intron 45.0%
ATGTAAACCCTTCCTCTGGT+TGG + chr2_4:21427230-21427249 None:intergenic 45.0%
TGGAATGTAAACCCTTCCTC+TGG + chr2_4:21427234-21427253 None:intergenic 45.0%
GCAGGAACCCAACTATACAA+GGG - chr2_4:21427432-21427451 Msa0320720:intron 45.0%
GAACCCAACTATACAAGGGA+TGG - chr2_4:21427436-21427455 Msa0320720:intron 45.0%
CACACCATCCCTTGTATAGT+TGG + chr2_4:21427443-21427462 None:intergenic 45.0%
GATGTCGTACCAAGCAGAAA+TGG - chr2_4:21427639-21427658 Msa0320720:intron 45.0%
!! CTAATTGGGGGCACCTAATA+TGG - chr2_4:21427826-21427845 Msa0320720:CDS 45.0%
! TTTTCCATTTCCTCCACCCA+CGG - chr2_4:21427874-21427893 Msa0320720:CDS 45.0%
CAATTAATAAGGACCGTGGG+TGG + chr2_4:21427890-21427909 None:intergenic 45.0%
CGGTCAATTAATAAGGACCG+TGG + chr2_4:21427894-21427913 None:intergenic 45.0%
CTGATGTGGCTTCACATGAG+AGG + chr2_4:21425308-21425327 None:intergenic 50.0%
CACCCTCAACACAACCTCTA+TGG - chr2_4:21425416-21425435 Msa0320720:three_prime_UTR 50.0%
GTCTCCTCTGTGAGCACTTA+TGG - chr2_4:21425479-21425498 Msa0320720:three_prime_UTR 50.0%
ACGACCATAAGTGCTCACAG+AGG + chr2_4:21425486-21425505 None:intergenic 50.0%
CACTTGGCCGTTTAGAGGTT+TGG + chr2_4:21426203-21426222 None:intergenic 50.0%
ACCTCTAAACGGCCAAGTGA+TGG - chr2_4:21426204-21426223 Msa0320720:intron 50.0%
TCCATCACTTGGCCGTTTAG+AGG + chr2_4:21426208-21426227 None:intergenic 50.0%
! CTAAACGGCCAAGTGATGGA+AGG - chr2_4:21426208-21426227 Msa0320720:intron 50.0%
TGTTGTGTCCAACCAGAGGA+AGG - chr2_4:21427219-21427238 Msa0320720:intron 50.0%
GTTGTGTCCAACCAGAGGAA+GGG - chr2_4:21427220-21427239 Msa0320720:intron 50.0%
GGCAGGAACCCAACTATACA+AGG - chr2_4:21427431-21427450 Msa0320720:intron 50.0%
ATACCAGTCACACTCAGTCG+TGG - chr2_4:21427611-21427630 Msa0320720:intron 50.0%
TTTCCACGACTGAGTGTGAC+TGG + chr2_4:21427617-21427636 None:intergenic 50.0%
TTAATAAGGACCGTGGGTGG+AGG + chr2_4:21427887-21427906 None:intergenic 50.0%
GGAGGCTAACGCAGTGACAA+GGG + chr2_4:21425456-21425475 None:intergenic 55.0%
AGTGCTCACAGAGGAGACGA+CGG + chr2_4:21425477-21425496 None:intergenic 55.0%
CGGAGGCTAACGCAGTGACA+AGG + chr2_4:21425457-21425476 None:intergenic 60.0%
AGGACCGTGGGTGGAGGAAA+TGG + chr2_4:21427881-21427900 None:intergenic 60.0%
GCTCACAGAGGAGACGACGG+AGG + chr2_4:21425474-21425493 None:intergenic 65.0%
Chromosome Type Strat End Strand Name
chr2_4 gene 21424935 21427916 21424935 ID=Msa0320720;Name=Msa0320720
chr2_4 mRNA 21424935 21427916 21424935 ID=Msa0320720-mRNA-1;Parent=Msa0320720;Name=Msa0320720-mRNA-1;_AED=0.41;_eAED=0.41;_QI=0|0.5|0.33|0.66|0.5|0.66|3|658|120
chr2_4 exon 21424935 21425704 21424935 ID=Msa0320720-mRNA-1:exon:7935;Parent=Msa0320720-mRNA-1
chr2_4 exon 21427287 21427404 21427287 ID=Msa0320720-mRNA-1:exon:7934;Parent=Msa0320720-mRNA-1
chr2_4 exon 21427784 21427916 21427784 ID=Msa0320720-mRNA-1:exon:7933;Parent=Msa0320720-mRNA-1
chr2_4 CDS 21427784 21427916 21427784 ID=Msa0320720-mRNA-1:cds;Parent=Msa0320720-mRNA-1
chr2_4 CDS 21427287 21427404 21427287 ID=Msa0320720-mRNA-1:cds;Parent=Msa0320720-mRNA-1
chr2_4 CDS 21425593 21425704 21425593 ID=Msa0320720-mRNA-1:cds;Parent=Msa0320720-mRNA-1
chr2_4 three_prime_UTR 21424935 21425592 21424935 ID=Msa0320720-mRNA-1:three_prime_utr;Parent=Msa0320720-mRNA-1
Gene Sequence

>Msa0320720

ATGATGTCAATTGCAATTATTCAGAACACTATACACTTCGTTGAAGCCATAGTCTTTCCTTACATAGCACTTGACTTTTTGCTTCTTGTTATTTTTACCTTTTGGCCTACTGATATGGATATGGTAGACTCAGATCTGTCCCTTGTCACTGCGTTAGCCTCCGTCGTCTCCTCTGTGAGCACTTATGGTCGTAACTGGTTCAATCGTCTTTGTCCTGAGATCTCTGTACCTTGTCACTGTGTTAGACTCAGCAACAATGTAGATGTTTATTTCTTTCACCAAGCTCACACAAAGAACAAGGTTGAAGAAAGAGCTACCAAGTATGTTGTGTCCAACCAGAGGAAGGGTTTACATTCCAATTGA
Protein sequence

>Msa0320720

MMSIAIIQNTIHFVEAIVFPYIALDFLLLVIFTFWPTDMDMVDSDLSLVTALASVVSSVSTYGRNWFNRLCPEISVPCHCVRLSNNVDVYFFHQAHTKNKVEERATKYVVSNQRKGLHSN*