AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa0281450

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score

Find 60 sgRNAs with CRISPR-Local

Find 192 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
CATTCCAACTTTGTACTTTA+AGG 0.047552 2_3:+16906282 None:intergenic
GATTCTGAGTTACAACTAAT+TGG 0.169825 2_3:-16905733 Msa0281450:three_prime_UTR
GTCTCCTCTGTGAGCACTTA+TGG 0.248211 2_3:-16908065 Msa0281450:CDS
ATTCAATCGGTCAATTAATA+AGG 0.287292 2_3:+16905646 None:intergenic
TAAAATAAATGCTTAAATAA+TGG 0.309201 2_3:+16906036 None:intergenic
ATTCCAACTTTGTACTTTAA+GGG 0.318235 2_3:+16906283 None:intergenic
TTGGCCTACTGATATGGATA+TGG 0.323907 2_3:-16908508 Msa0281450:CDS
ATTCTGAGTTACAACTAATT+GGG 0.330830 2_3:-16905732 Msa0281450:three_prime_UTR
TAGGGACTCGAATTCTTAAT+AGG 0.375298 2_3:-16906134 Msa0281450:three_prime_UTR
TATGAAAAGTTGAATATTGT+TGG 0.378727 2_3:-16906188 Msa0281450:three_prime_UTR
CCTTGTTCTTTGTGTGAGCT+TGG 0.386697 2_3:+16906370 None:intergenic
CACACCATCCCTTGTATAGT+TGG 0.388307 2_3:+16906104 None:intergenic
GTGTAAAAGAGGTCCATATT+AGG 0.394434 2_3:+16905705 None:intergenic
ACTGCTTCTAATTACATGAT+AGG 0.394642 2_3:+16905806 None:intergenic
AATGCTAAATTAAACTATAT+AGG 0.416900 2_3:-16906242 Msa0281450:three_prime_UTR
AGGACCGTGGGTGGAGGAAA+TGG 0.432827 2_3:+16905666 None:intergenic
GTTGAATATTGTTGGAACAA+TGG 0.446379 2_3:-16906180 Msa0281450:three_prime_UTR
TGAGCACTTATGGTCGTAAC+TGG 0.451215 2_3:-16908055 Msa0281450:CDS
GATGTCGTACCAAGCAGAAA+TGG 0.452468 2_3:-16905905 Msa0281450:three_prime_UTR
TGCTATGTAAGGAAAGACTA+TGG 0.456105 2_3:+16908563 None:intergenic
TTAAAGTACAAAGTTGGAAT+GGG 0.460423 2_3:-16906280 Msa0281450:three_prime_UTR
TGTACTTTAAGGGCTTCAAT+TGG 0.463577 2_3:+16906293 None:intergenic
CTGGTTGGACACAACATACT+TGG 0.464582 2_3:+16906332 None:intergenic
CTTAAAGTACAAAGTTGGAA+TGG 0.466864 2_3:-16906281 Msa0281450:three_prime_UTR
AAGCCCTTAAAGTACAAAGT+TGG 0.470446 2_3:-16906286 Msa0281450:three_prime_UTR
ACACCATCCCTTGTATAGTT+GGG 0.474764 2_3:+16906105 None:intergenic
ATCCATATCAGTAGGCCAAA+AGG 0.488360 2_3:+16908512 None:intergenic
GATCAAGATAGCATAGGGAT+AGG 0.491298 2_3:-16906153 Msa0281450:three_prime_UTR
TGGAATGTAAACCCTTCCTC+TGG 0.495186 2_3:+16906313 None:intergenic
CTTGTCACTGTGTTAGACTC+AGG 0.497067 2_3:-16908001 Msa0281450:intron
GACTCGAATTCTTAATAGGC+AGG 0.502520 2_3:-16906130 Msa0281450:three_prime_UTR
TGATCTGAACAGTGTAAAAG+AGG 0.509203 2_3:+16905694 None:intergenic
TTTCCACGACTGAGTGTGAC+TGG 0.512586 2_3:+16905930 None:intergenic
GGCAGGAACCCAACTATACA+AGG 0.534735 2_3:-16906113 Msa0281450:three_prime_UTR
TTAATAAGGACCGTGGGTGG+AGG 0.546474 2_3:+16905660 None:intergenic
ATCAAGATAGCATAGGGATA+GGG 0.551154 2_3:-16906152 Msa0281450:three_prime_UTR
TTCTGAGTTACAACTAATTG+GGG 0.555243 2_3:-16905731 Msa0281450:three_prime_UTR
CGGAGGCTAACGCAGTGACA+AGG 0.558881 2_3:+16908090 None:intergenic
TGTTGTGTCCAACCAGAGGA+AGG 0.568465 2_3:-16906325 Msa0281450:CDS
ATGTAAACCCTTCCTCTGGT+TGG 0.582925 2_3:+16906317 None:intergenic
TGACAAGGTACAGAGATCTC+AGG 0.588071 2_3:+16908017 None:intergenic
AGCAGCAAATCTACATACAT+AGG 0.596540 2_3:+16905964 None:intergenic
CCAAGCTCACACAAAGAACA+AGG 0.605053 2_3:-16906370 Msa0281450:CDS
CAATTAATAAGGACCGTGGG+TGG 0.605970 2_3:+16905657 None:intergenic
GTTGTGTCCAACCAGAGGAA+GGG 0.610598 2_3:-16906324 Msa0281450:CDS
GAACCCAACTATACAAGGGA+TGG 0.613369 2_3:-16906108 Msa0281450:three_prime_UTR
CGGTCAATTAATAAGGACCG+TGG 0.615141 2_3:+16905653 None:intergenic
AGAAGTTGTATCGATGACAT+AGG 0.615984 2_3:-16905995 Msa0281450:three_prime_UTR
GATATGGATATGGTAGACTC+AGG 0.623990 2_3:-16908498 Msa0281450:intron
TCTACCATATCCATATCAGT+AGG 0.628700 2_3:+16908504 None:intergenic
ATACCAGTCACACTCAGTCG+TGG 0.635423 2_3:-16905933 Msa0281450:three_prime_UTR
GGAGGCTAACGCAGTGACAA+GGG 0.635819 2_3:+16908091 None:intergenic
CTGAGTCTAACACAGTGACA+AGG 0.661348 2_3:+16908002 None:intergenic
GGTCAATTAATAAGGACCGT+GGG 0.678476 2_3:+16905654 None:intergenic
AGTATGTTGTGTCCAACCAG+AGG 0.686559 2_3:-16906329 Msa0281450:CDS
AGTGCTCACAGAGGAGACGA+CGG 0.708102 2_3:+16908070 None:intergenic
GCAGGAACCCAACTATACAA+GGG 0.709629 2_3:-16906112 Msa0281450:three_prime_UTR
GCTCACAGAGGAGACGACGG+AGG 0.740555 2_3:+16908073 None:intergenic
TCTGAGTTACAACTAATTGG+GGG 0.747189 2_3:-16905730 Msa0281450:three_prime_UTR
ACGACCATAAGTGCTCACAG+AGG 0.812409 2_3:+16908061 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!!! ATTTTTTATTTTTTTATTTT+TGG + chr2_3:16906874-16906893 None:intergenic 0.0%
!! AAACAAAAAAAAAAAACTTA+TGG + chr2_3:16906093-16906112 None:intergenic 10.0%
!!! CTTAAAATTTTAAAACATAA+AGG + chr2_3:16906666-16906685 None:intergenic 10.0%
!!! TATGTTTTAAAATTTTAAGT+TGG - chr2_3:16906667-16906686 Msa0281450:intron 10.0%
!! AAAACTTAAAATAAACAAAA+GGG - chr2_3:16907016-16907035 Msa0281450:intron 10.0%
!! AAAAAAAAACTCAAATAATT+AGG + chr2_3:16907356-16907375 None:intergenic 10.0%
!! ATGATAATTTAAAAGAAATA+TGG + chr2_3:16907799-16907818 None:intergenic 10.0%
!!! TATTTCTTTTAAATTATCAT+AGG - chr2_3:16907799-16907818 Msa0281450:intron 10.0%
!! TAAAATAAATGCTTAAATAA+TGG + chr2_3:16908226-16908245 None:intergenic 10.0%
!! TTTAAATATCATTAGTTCAT+AGG - chr2_3:16905784-16905803 Msa0281450:three_prime_UTR 15.0%
!!! CTTTTATTTTATTTCTTTAC+CGG - chr2_3:16906044-16906063 Msa0281450:three_prime_UTR 15.0%
!!! GTTTATTTTAGTTTTTAAGT+AGG - chr2_3:16906639-16906658 Msa0281450:intron 15.0%
!!! TTAAAGCTTTTAAAACATAA+GGG - chr2_3:16906985-16907004 Msa0281450:intron 15.0%
!!! TTTATTTTAAGTTTTGAGTT+GGG + chr2_3:16907011-16907030 None:intergenic 15.0%
!! CAAAACTTAAAATAAACAAA+AGG - chr2_3:16907015-16907034 Msa0281450:intron 15.0%
!!! TATATTTATGTTTTAAAGCT+AGG - chr2_3:16907673-16907692 Msa0281450:intron 15.0%
!! AATGCTAAATTAAACTATAT+AGG - chr2_3:16908017-16908036 Msa0281450:CDS 15.0%
!!! TTCTTGTTATTTTTACCTTT+TGG - chr2_3:16905732-16905751 Msa0281450:three_prime_UTR 20.0%
!!! GTGTTATTTTGATATAGATA+TGG - chr2_3:16905806-16905825 Msa0281450:three_prime_UTR 20.0%
!! CGGTAAAGAAATAAAATAAA+AGG + chr2_3:16906046-16906065 None:intergenic 20.0%
!!! TTCAAGACTATTTAACTTTA+AGG - chr2_3:16906406-16906425 Msa0281450:intron 20.0%
!!! GTAAAACATTGGTTTAATAA+AGG + chr2_3:16906558-16906577 None:intergenic 20.0%
!! AAAAACTAAAATAAACGTGA+GGG + chr2_3:16906635-16906654 None:intergenic 20.0%
!! TAAAAACTAAAATAAACGTG+AGG + chr2_3:16906636-16906655 None:intergenic 20.0%
!! TTACTAAACTCAATATTATG+TGG + chr2_3:16906763-16906782 None:intergenic 20.0%
!!! TTTATTTTTGGAATGAAAGA+GGG + chr2_3:16906862-16906881 None:intergenic 20.0%
!!! TTTTATTTTTGGAATGAAAG+AGG + chr2_3:16906863-16906882 None:intergenic 20.0%
!!! TTCCAATATGATTAACATTT+TGG - chr2_3:16906956-16906975 Msa0281450:intron 20.0%
!!! CTTAAAGCTTTTAAAACATA+AGG - chr2_3:16906984-16907003 Msa0281450:intron 20.0%
!!! GTTTATTTTAAGTTTTGAGT+TGG + chr2_3:16907012-16907031 None:intergenic 20.0%
!!! ATGTTATAAGCACTTTTAAT+TGG - chr2_3:16907161-16907180 Msa0281450:intron 20.0%
!!! CACTTTTAATTGGATAAATA+AGG - chr2_3:16907171-16907190 Msa0281450:intron 20.0%
!! ATAAGTTCTATAGTAGTATA+TGG - chr2_3:16907540-16907559 Msa0281450:intron 20.0%
!! TATGAAAAGTTGAATATTGT+TGG - chr2_3:16908071-16908090 Msa0281450:CDS 20.0%
!! GACTTCTATTAACAAAAAAA+AGG + chr2_3:16908416-16908435 None:intergenic 20.0%
! TATCATATATTCTTTGCTGA+AGG - chr2_3:16905845-16905864 Msa0281450:three_prime_UTR 25.0%
! TAAGTTCTATAGTAGTATCA+TGG - chr2_3:16905911-16905930 Msa0281450:three_prime_UTR 25.0%
! ATAAGTGTCTGCAAATAAAA+GGG - chr2_3:16905950-16905969 Msa0281450:three_prime_UTR 25.0%
!!! TGTTATAATGAGTGTTTTGA+GGG + chr2_3:16905997-16906016 None:intergenic 25.0%
! AATAAAATAAAAGGCTGATG+TGG + chr2_3:16906037-16906056 None:intergenic 25.0%
!!! ATTATTTTGATTAACTGCCA+TGG - chr2_3:16906304-16906323 Msa0281450:exon 25.0%
!!! TTATTTTGATTAACTGCCAT+GGG - chr2_3:16906305-16906324 Msa0281450:exon 25.0%
!!! TAAGCTTGTAGAGTATTTTA+TGG - chr2_3:16906484-16906503 Msa0281450:intron 25.0%
!!! AAGCTTGTAGAGTATTTTAT+GGG - chr2_3:16906485-16906504 Msa0281450:intron 25.0%
! CAAAATAGGATGTAAAACAT+TGG + chr2_3:16906569-16906588 None:intergenic 25.0%
!!! TATGCACACGTATAATTTTT+TGG - chr2_3:16906591-16906610 Msa0281450:intron 25.0%
! AAAACTAAAATAAACGTGAG+GGG + chr2_3:16906634-16906653 None:intergenic 25.0%
!!! TTGTTTTAAGAGTTTCAAGT+AGG - chr2_3:16906696-16906715 Msa0281450:intron 25.0%
!! TAAATTCCAACACTCATTTT+TGG - chr2_3:16906829-16906848 Msa0281450:intron 25.0%
!!! AAATTCCAACACTCATTTTT+GGG - chr2_3:16906830-16906849 Msa0281450:intron 25.0%
! GTCCAAAATGTTAATCATAT+TGG + chr2_3:16906961-16906980 None:intergenic 25.0%
!! GCTATGTTTCATTTGATTTA+AGG - chr2_3:16907216-16907235 Msa0281450:intron 25.0%
!! CATTTGATTTAAGGCATATA+TGG - chr2_3:16907225-16907244 Msa0281450:intron 25.0%
! GTACAACAATTCAAATAACT+TGG + chr2_3:16907276-16907295 None:intergenic 25.0%
!! ATCAGCATGTATTCTTTTTA+TGG - chr2_3:16907329-16907348 Msa0281450:intron 25.0%
! ATGATGATATACTTTCTTTG+AGG - chr2_3:16907506-16907525 Msa0281450:intron 25.0%
! ACAAGTGTCTTCAAATAAAA+GGG - chr2_3:16907579-16907598 Msa0281450:intron 25.0%
! GAAACAAATCTATTAGCAAT+TGG - chr2_3:16907649-16907668 Msa0281450:intron 25.0%
! ACAACCATTTACATTCAAAA+GGG + chr2_3:16907766-16907785 None:intergenic 25.0%
! ATTCCAACTTTGTACTTTAA+GGG + chr2_3:16907979-16907998 None:intergenic 25.0%
! TTAAAGTACAAAGTTGGAAT+GGG - chr2_3:16907979-16907998 Msa0281450:intron 25.0%
!!! TGTTGTTTTACTTACATGAA+TGG - chr2_3:16908046-16908065 Msa0281450:CDS 25.0%
! ATTCTGAGTTACAACTAATT+GGG - chr2_3:16908527-16908546 Msa0281450:CDS 25.0%
AAAAAGTCAAGTGCTATGTA+AGG + chr2_3:16905710-16905729 None:intergenic 30.0%
GATATAGATATGGTATACTC+AGG - chr2_3:16905816-16905835 Msa0281450:three_prime_UTR 30.0%
ATGATGATATACTTTCCTTG+AGG - chr2_3:16905880-16905899 Msa0281450:three_prime_UTR 30.0%
GATAAGTGTCTGCAAATAAA+AGG - chr2_3:16905949-16905968 Msa0281450:three_prime_UTR 30.0%
! GTGTTATAATGAGTGTTTTG+AGG + chr2_3:16905998-16906017 None:intergenic 30.0%
! TTTATTTCTTTACCGGCTAA+TGG - chr2_3:16906051-16906070 Msa0281450:three_prime_UTR 30.0%
GATGTAGTCCTATAAAATAC+AGG + chr2_3:16906352-16906371 None:intergenic 30.0%
ACATATTAGATAGAGGTTGA+AGG + chr2_3:16906533-16906552 None:intergenic 30.0%
AAAGGTCACATATTAGATAG+AGG + chr2_3:16906540-16906559 None:intergenic 30.0%
TACGTGTGCATAAACAAAAT+AGG + chr2_3:16906583-16906602 None:intergenic 30.0%
!!! TTTTGGCTTAATTGCATGTT+TGG - chr2_3:16906608-16906627 Msa0281450:intron 30.0%
! TTCCAAAATGAGTAACGTTT+TGG + chr2_3:16906723-16906742 None:intergenic 30.0%
CTAAACTCAATATTATGTGG+CGG + chr2_3:16906760-16906779 None:intergenic 30.0%
AATAGGCTTAATTGCATGTT+TGG + chr2_3:16907043-16907062 None:intergenic 30.0%
CCTTTACAAGGTAAAAGAAT+AGG + chr2_3:16907060-16907079 None:intergenic 30.0%
! CCTATTCTTTTACCTTGTAA+AGG - chr2_3:16907057-16907076 Msa0281450:intron 30.0%
CAATTCAAATAACTTGGTTG+AGG + chr2_3:16907270-16907289 None:intergenic 30.0%
AATCAAACAAACAACAAGCT+TGG + chr2_3:16907446-16907465 None:intergenic 30.0%
!! TGATATACTTTCTTTGAGGT+TGG - chr2_3:16907510-16907529 Msa0281450:intron 30.0%
GACAAGTGTCTTCAAATAAA+AGG - chr2_3:16907578-16907597 Msa0281450:intron 30.0%
AAGGGATATATTTACCTCTA+AGG - chr2_3:16907597-16907616 Msa0281450:intron 30.0%
GACAACCATTTACATTCAAA+AGG + chr2_3:16907767-16907786 None:intergenic 30.0%
CATTCCAACTTTGTACTTTA+AGG + chr2_3:16907980-16907999 None:intergenic 30.0%
CTTAAAGTACAAAGTTGGAA+TGG - chr2_3:16907978-16907997 Msa0281450:intron 30.0%
GTTGAATATTGTTGGAACAA+TGG - chr2_3:16908079-16908098 Msa0281450:CDS 30.0%
!! GTTTTGATCAAGATAGCATA+GGG - chr2_3:16908101-16908120 Msa0281450:intron 30.0%
!!! TTTTGAGTTGCTTAAAGAAC+TGG - chr2_3:16908380-16908399 Msa0281450:intron 30.0%
ACTGCTTCTAATTACATGAT+AGG + chr2_3:16908456-16908475 None:intergenic 30.0%
GATTCTGAGTTACAACTAAT+TGG - chr2_3:16908526-16908545 Msa0281450:CDS 30.0%
TTCTGAGTTACAACTAATTG+GGG - chr2_3:16908528-16908547 Msa0281450:CDS 30.0%
TGCTATGTAAGGAAAGACTA+TGG + chr2_3:16905699-16905718 None:intergenic 35.0%
TCTACCATATCCATATCAGT+AGG + chr2_3:16905758-16905777 None:intergenic 35.0%
TATTCTTTGCTGAAGGAGTA+TGG - chr2_3:16905852-16905871 Msa0281450:three_prime_UTR 35.0%
GAACTTATCATTCAACCTCA+AGG + chr2_3:16905898-16905917 None:intergenic 35.0%
AAAACTTATGGCTCAGAACT+TGG + chr2_3:16906081-16906100 None:intergenic 35.0%
! GTCCATGTCCTGTATTTTAT+AGG - chr2_3:16906341-16906360 Msa0281450:CDS 35.0%
GTCCTATAAAATACAGGACA+TGG + chr2_3:16906346-16906365 None:intergenic 35.0%
! ATATTTTCTCGTGCACAAAC+TGG - chr2_3:16906454-16906473 Msa0281450:intron 35.0%
! GTCCAAAACGTTACTCATTT+TGG - chr2_3:16906718-16906737 Msa0281450:intron 35.0%
GGAAAAATCTCTCCTTTACA+AGG + chr2_3:16907072-16907091 None:intergenic 35.0%
TTCCAAGTATCTCACCTATA+AGG - chr2_3:16907088-16907107 Msa0281450:intron 35.0%
GACTCTTTACTGAAAGAGTA+TGG - chr2_3:16907466-16907485 Msa0281450:intron 35.0%
! GGAAGCACATGAACTATATT+GGG + chr2_3:16907726-16907745 None:intergenic 35.0%
! TGGTCCCTTTTGAATGTAAA+TGG - chr2_3:16907759-16907778 Msa0281450:intron 35.0%
! TGTACTTTAAGGGCTTCAAT+TGG + chr2_3:16907969-16907988 None:intergenic 35.0%
AAGCCCTTAAAGTACAAAGT+TGG - chr2_3:16907973-16907992 Msa0281450:intron 35.0%
!! GGTTTTGATCAAGATAGCAT+AGG - chr2_3:16908100-16908119 Msa0281450:CDS 35.0%
ATCAAGATAGCATAGGGATA+GGG - chr2_3:16908107-16908126 Msa0281450:intron 35.0%
TAGGGACTCGAATTCTTAAT+AGG - chr2_3:16908125-16908144 Msa0281450:intron 35.0%
! AGAAGTTGTATCGATGACAT+AGG - chr2_3:16908264-16908283 Msa0281450:intron 35.0%
AGCAGCAAATCTACATACAT+AGG + chr2_3:16908298-16908317 None:intergenic 35.0%
TCAAAAAAGCCATTTCTGCT+TGG + chr2_3:16908366-16908385 None:intergenic 35.0%
TCTGAGTTACAACTAATTGG+GGG - chr2_3:16908529-16908548 Msa0281450:CDS 35.0%
GTGTAAAAGAGGTCCATATT+AGG + chr2_3:16908557-16908576 None:intergenic 35.0%
TGATCTGAACAGTGTAAAAG+AGG + chr2_3:16908568-16908587 None:intergenic 35.0%
! TACCTTTTGGCCTACTGATA+TGG - chr2_3:16905745-16905764 Msa0281450:three_prime_UTR 40.0%
ATCCATATCAGTAGGCCAAA+AGG + chr2_3:16905750-16905769 None:intergenic 40.0%
TTGGCCTACTGATATGGATA+TGG - chr2_3:16905751-16905770 Msa0281450:three_prime_UTR 40.0%
GATATGGATATGGTAGACTC+AGG - chr2_3:16905761-16905780 Msa0281450:three_prime_UTR 40.0%
GTCTGCAAATAAAAGGGATG+AGG - chr2_3:16905956-16905975 Msa0281450:three_prime_UTR 40.0%
TAAAAGGGATGAGGAATGCT+AGG - chr2_3:16905965-16905984 Msa0281450:three_prime_UTR 40.0%
AAAAGGGATGAGGAATGCTA+GGG - chr2_3:16905966-16905985 Msa0281450:three_prime_UTR 40.0%
GAACTTGGTTAACCATTAGC+CGG + chr2_3:16906066-16906085 None:intergenic 40.0%
CACGAGAAAATATGCGAAAG+TGG + chr2_3:16906447-16906466 None:intergenic 40.0%
! CTCAATATTATGTGGCGGAA+GGG + chr2_3:16906755-16906774 None:intergenic 40.0%
! ACTCAATATTATGTGGCGGA+AGG + chr2_3:16906756-16906775 None:intergenic 40.0%
TGAAGCCCAAAAATGAGTGT+TGG + chr2_3:16906838-16906857 None:intergenic 40.0%
TGCCTTATAGGTGAGATACT+TGG + chr2_3:16907093-16907112 None:intergenic 40.0%
! CTTTTGTATGTGCCCTACTA+TGG - chr2_3:16907300-16907319 Msa0281450:intron 40.0%
! GCTGATTTAGTGCCATAGTA+GGG + chr2_3:16907315-16907334 None:intergenic 40.0%
!! TGCTGATTTAGTGCCATAGT+AGG + chr2_3:16907316-16907335 None:intergenic 40.0%
! GTAATGTTGCTTCACCTTAG+AGG + chr2_3:16907614-16907633 None:intergenic 40.0%
AGGTGAAGCAACATTACGTA+TGG - chr2_3:16907617-16907636 Msa0281450:intron 40.0%
AGCACATGAACTATATTGGG+CGG + chr2_3:16907723-16907742 None:intergenic 40.0%
! CGGAAGCACATGAACTATAT+TGG + chr2_3:16907727-16907746 None:intergenic 40.0%
TGCTTCCGAAAATTGCTATG+TGG - chr2_3:16907739-16907758 Msa0281450:intron 40.0%
! AGGGACCACATAGCAATTTT+CGG + chr2_3:16907747-16907766 None:intergenic 40.0%
GATCAAGATAGCATAGGGAT+AGG - chr2_3:16908106-16908125 Msa0281450:intron 40.0%
GACTCGAATTCTTAATAGGC+AGG - chr2_3:16908129-16908148 Msa0281450:intron 40.0%
ACACCATCCCTTGTATAGTT+GGG + chr2_3:16908157-16908176 None:intergenic 40.0%
GGTCAATTAATAAGGACCGT+GGG + chr2_3:16908608-16908627 None:intergenic 40.0%
TGATGTGGCTTCACATGAGA+GGG + chr2_3:16906022-16906041 None:intergenic 45.0%
AGCCATAGAGGTTGTGTTGA+GGG + chr2_3:16906136-16906155 None:intergenic 45.0%
AAGCCATAGAGGTTGTGTTG+AGG + chr2_3:16906137-16906156 None:intergenic 45.0%
ACAGATCTGCAAAGCCATAG+AGG + chr2_3:16906148-16906167 None:intergenic 45.0%
TGAGCACTTATGGTCGTAAC+TGG - chr2_3:16906204-16906223 Msa0281450:three_prime_UTR 45.0%
TGACAAGGTACAGAGATCTC+AGG + chr2_3:16906245-16906264 None:intergenic 45.0%
CTGAGTCTAACACAGTGACA+AGG + chr2_3:16906260-16906279 None:intergenic 45.0%
CTTGTCACTGTGTTAGACTC+AGG - chr2_3:16906258-16906277 Msa0281450:three_prime_UTR 45.0%
GACAACATAGTACACACCCA+TGG + chr2_3:16906324-16906343 None:intergenic 45.0%
TGGGCTGAGAATATGAGTGT+TGG + chr2_3:16906898-16906917 None:intergenic 45.0%
TCTCAGCCCAAACCTCTAAA+CGG - chr2_3:16906908-16906927 Msa0281450:intron 45.0%
ACTTGGCCGTTTAGAGGTTT+GGG + chr2_3:16906917-16906936 None:intergenic 45.0%
! TAAACGGCCAAGTGATGGAA+GGG - chr2_3:16906924-16906943 Msa0281450:intron 45.0%
AGTTAGTCCCTTCCATCACT+TGG + chr2_3:16906934-16906953 None:intergenic 45.0%
GAAGCTTCTTGCTGCCTTAT+AGG + chr2_3:16907105-16907124 None:intergenic 45.0%
CCTTGTTCTTTGTGTGAGCT+TGG + chr2_3:16907892-16907911 None:intergenic 45.0%
CCAAGCTCACACAAAGAACA+AGG - chr2_3:16907889-16907908 Msa0281450:intron 45.0%
CTGGTTGGACACAACATACT+TGG + chr2_3:16907930-16907949 None:intergenic 45.0%
AGTATGTTGTGTCCAACCAG+AGG - chr2_3:16907930-16907949 Msa0281450:intron 45.0%
ATGTAAACCCTTCCTCTGGT+TGG + chr2_3:16907945-16907964 None:intergenic 45.0%
TGGAATGTAAACCCTTCCTC+TGG + chr2_3:16907949-16907968 None:intergenic 45.0%
GCAGGAACCCAACTATACAA+GGG - chr2_3:16908147-16908166 Msa0281450:intron 45.0%
GAACCCAACTATACAAGGGA+TGG - chr2_3:16908151-16908170 Msa0281450:intron 45.0%
CACACCATCCCTTGTATAGT+TGG + chr2_3:16908158-16908177 None:intergenic 45.0%
GATGTCGTACCAAGCAGAAA+TGG - chr2_3:16908354-16908373 Msa0281450:intron 45.0%
!! CTAATTGGGGGCACCTAATA+TGG - chr2_3:16908541-16908560 Msa0281450:CDS 45.0%
! TTTTCCATTTCCTCCACCCA+CGG - chr2_3:16908589-16908608 Msa0281450:CDS 45.0%
CAATTAATAAGGACCGTGGG+TGG + chr2_3:16908605-16908624 None:intergenic 45.0%
CGGTCAATTAATAAGGACCG+TGG + chr2_3:16908609-16908628 None:intergenic 45.0%
CTGATGTGGCTTCACATGAG+AGG + chr2_3:16906023-16906042 None:intergenic 50.0%
CACCCTCAACACAACCTCTA+TGG - chr2_3:16906131-16906150 Msa0281450:three_prime_UTR 50.0%
GTCTCCTCTGTGAGCACTTA+TGG - chr2_3:16906194-16906213 Msa0281450:three_prime_UTR 50.0%
ACGACCATAAGTGCTCACAG+AGG + chr2_3:16906201-16906220 None:intergenic 50.0%
CACTTGGCCGTTTAGAGGTT+TGG + chr2_3:16906918-16906937 None:intergenic 50.0%
ACCTCTAAACGGCCAAGTGA+TGG - chr2_3:16906919-16906938 Msa0281450:intron 50.0%
TCCATCACTTGGCCGTTTAG+AGG + chr2_3:16906923-16906942 None:intergenic 50.0%
! CTAAACGGCCAAGTGATGGA+AGG - chr2_3:16906923-16906942 Msa0281450:intron 50.0%
TGTTGTGTCCAACCAGAGGA+AGG - chr2_3:16907934-16907953 Msa0281450:intron 50.0%
GTTGTGTCCAACCAGAGGAA+GGG - chr2_3:16907935-16907954 Msa0281450:intron 50.0%
GGCAGGAACCCAACTATACA+AGG - chr2_3:16908146-16908165 Msa0281450:intron 50.0%
ATACCAGTCACACTCAGTCG+TGG - chr2_3:16908326-16908345 Msa0281450:intron 50.0%
TTTCCACGACTGAGTGTGAC+TGG + chr2_3:16908332-16908351 None:intergenic 50.0%
TTAATAAGGACCGTGGGTGG+AGG + chr2_3:16908602-16908621 None:intergenic 50.0%
GGAGGCTAACGCAGTGACAA+GGG + chr2_3:16906171-16906190 None:intergenic 55.0%
AGTGCTCACAGAGGAGACGA+CGG + chr2_3:16906192-16906211 None:intergenic 55.0%
CGGAGGCTAACGCAGTGACA+AGG + chr2_3:16906172-16906191 None:intergenic 60.0%
AGGACCGTGGGTGGAGGAAA+TGG + chr2_3:16908596-16908615 None:intergenic 60.0%
GCTCACAGAGGAGACGACGG+AGG + chr2_3:16906189-16906208 None:intergenic 65.0%
Chromosome Type Strat End Strand Name
chr2_3 gene 16905650 16908631 16905650 ID=Msa0281450;Name=Msa0281450
chr2_3 mRNA 16905650 16908631 16905650 ID=Msa0281450-mRNA-1;Parent=Msa0281450;Name=Msa0281450-mRNA-1;_AED=0.41;_eAED=0.41;_QI=0|0.5|0.33|0.66|0.5|1|3|658|120
chr2_3 exon 16905650 16906419 16905650 ID=Msa0281450-mRNA-1:exon:7007;Parent=Msa0281450-mRNA-1
chr2_3 exon 16908002 16908119 16908002 ID=Msa0281450-mRNA-1:exon:7006;Parent=Msa0281450-mRNA-1
chr2_3 exon 16908499 16908631 16908499 ID=Msa0281450-mRNA-1:exon:7005;Parent=Msa0281450-mRNA-1
chr2_3 CDS 16908499 16908631 16908499 ID=Msa0281450-mRNA-1:cds;Parent=Msa0281450-mRNA-1
chr2_3 CDS 16908002 16908119 16908002 ID=Msa0281450-mRNA-1:cds;Parent=Msa0281450-mRNA-1
chr2_3 CDS 16906308 16906419 16906308 ID=Msa0281450-mRNA-1:cds;Parent=Msa0281450-mRNA-1
chr2_3 three_prime_UTR 16905650 16906307 16905650 ID=Msa0281450-mRNA-1:three_prime_utr;Parent=Msa0281450-mRNA-1
Gene Sequence

>Msa0281450

ATGATGTCAATTGCAATTATTCAGAACACTATACACTTCGTTGAAGCCATAGTCTTTCCTTACATAGCACTTGACTTTTTGCTTCTTGTTATTTTTACCTTTTGGCCTACTGATATGGATATGGTAGACTCAGATCTGTCCCTTGTCACTGCGTTAGCCTCCGTCGTCTCCTCTGTGAGCACTTATGGTCGTAACTGGTTCAATCGTCTTTGTCCTGAGATCTCTGTACCTTGTCACTGTGTTAGACTCAGCAACAATGTAGATGTTTATTTCTTTCACCAAGCTCACACAAAGAACAAGGTTGAAGAAAGAGCTACCAAGTATGTTGTGTCCAACCAGAGGAAGGGTTTACATTCCAATTGA
Protein sequence

>Msa0281450

MMSIAIIQNTIHFVEAIVFPYIALDFLLLVIFTFWPTDMDMVDSDLSLVTALASVVSSVSTYGRNWFNRLCPEISVPCHCVRLSNNVDVYFFHQAHTKNKVEERATKYVVSNQRKGLHSN*