Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Co-expression Network
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| Msa0029260 | Msa0327140 | -0.813661 | 2.286417e-51 | -8.615850e-47 |
| Msa0052350 | Msa0327140 | 0.826660 | 2.401885e-54 | -8.615850e-47 |
| Msa0097360 | Msa0327140 | 0.822769 | 1.983815e-53 | -8.615850e-47 |
| Msa0169300 | Msa0327140 | -0.808226 | 3.436357e-50 | -8.615850e-47 |
| Msa0231790 | Msa0327140 | -0.814274 | 1.674801e-51 | -8.615850e-47 |
| Msa0289310 | Msa0327140 | 0.814570 | 1.440321e-51 | -8.615850e-47 |
| Msa0327140 | Msa0419520 | -0.803867 | 2.839548e-49 | -8.615850e-47 |
| Msa0327140 | Msa0602370 | -0.807057 | 6.085655e-50 | -8.615850e-47 |
| Msa0327140 | Msa0609000 | -0.804449 | 2.148625e-49 | -8.615850e-47 |
| Msa0327140 | Msa0651930 | -0.815773 | 7.785605e-52 | -8.615850e-47 |
Find 455 sgRNAs with CRISPR-Local
Find 853 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| AAAAGATCCGTCTCACGTAC+TGG | 0.504134 | 2_4:+38631246 | Msa0327140:three_prime_UTR |
| AAAATCAAATTTCTTCAAAG+AGG | 0.489318 | 2_4:-38634660 | None:intergenic |
| AAACAACAGAGGAGATATCT+TGG | 0.449393 | 2_4:-38636438 | None:intergenic |
| AAAGATGTGGAGCTTGGAAG+CGG | 0.580820 | 2_4:-38634098 | None:intergenic |
| AAAGTTGACTAAACTTGTGA+TGG | 0.567038 | 2_4:+38635168 | Msa0327140:three_prime_UTR |
| AAATAAACATGGTGAGGTGG+TGG | 0.519829 | 2_4:-38628135 | None:intergenic |
| AAATAAACCCCGTCAGGAGA+CGG | 0.574336 | 2_4:-38628321 | None:intergenic |
| AAATGCTGAAAATGGGGCGG+CGG | 0.601582 | 2_4:-38628108 | None:intergenic |
| AAATGGGGCGGCGGCGGAGG+AGG | 0.371976 | 2_4:-38628099 | None:intergenic |
| AAATTCTCATCAGCAGCACT+TGG | 0.544261 | 2_4:+38633206 | Msa0327140:three_prime_UTR |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| TTGTCCTATAAATGTAGAAG+AGG | + | chr2_4:38626287-38626306 | Msa0327140:intron | 30.0% | |
| TATTCAATCTTCACAACAGA+TGG | - | chr2_4:38626487-38626506 | None:intergenic | 30.0% | |
| GACAATAGTTATGACAAGAA+AGG | + | chr2_4:38626595-38626614 | Msa0327140:CDS | 30.0% | |
| CTCTGTGAACTTATTTCAAT+TGG | + | chr2_4:38627171-38627190 | Msa0327140:intron | 30.0% | |
| AAATATACTCTGATAATCGG+AGG | + | chr2_4:38627302-38627321 | Msa0327140:intron | 30.0% | |
| ATTGAATTCACTTCACACAT+TGG | - | chr2_4:38627817-38627836 | None:intergenic | 30.0% | |
| CATGTCCATGTAAATAAACA+TGG | - | chr2_4:38628149-38628168 | None:intergenic | 30.0% | |
| CATGTTTATTTACATGGACA+TGG | + | chr2_4:38628147-38628166 | Msa0327140:three_prime_UTR | 30.0% | |
| ATTATGCAACATTTGTCCTT+GGG | - | chr2_4:38628262-38628281 | None:intergenic | 30.0% | |
| AATTATGATATACTTGGCTC+TGG | + | chr2_4:38628720-38628739 | Msa0327140:intron | 30.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr2_4 | gene | 38626221 | 38636897 | 38626221 | ID=Msa0327140;Name=Msa0327140 |
| chr2_4 | mRNA | 38626221 | 38636897 | 38626221 | ID=Msa0327140-mRNA-1;Parent=Msa0327140;Name=Msa0327140-mRNA-1;_AED=0.13;_eAED=0.13;_QI=0|0.71|0.75|0.75|0.71|0.5|8|5035|106 |
| chr2_4 | exon | 38626221 | 38626225 | 38626221 | ID=Msa0327140-mRNA-1:exon:11578;Parent=Msa0327140-mRNA-1 |
| chr2_4 | exon | 38626522 | 38626770 | 38626522 | ID=Msa0327140-mRNA-1:exon:11579;Parent=Msa0327140-mRNA-1 |
| chr2_4 | exon | 38627918 | 38628675 | 38627918 | ID=Msa0327140-mRNA-1:exon:11580;Parent=Msa0327140-mRNA-1 |
| chr2_4 | exon | 38629537 | 38629743 | 38629537 | ID=Msa0327140-mRNA-1:exon:11581;Parent=Msa0327140-mRNA-1 |
| chr2_4 | exon | 38630746 | 38631652 | 38630746 | ID=Msa0327140-mRNA-1:exon:11582;Parent=Msa0327140-mRNA-1 |
| chr2_4 | exon | 38632672 | 38635189 | 38632672 | ID=Msa0327140-mRNA-1:exon:11583;Parent=Msa0327140-mRNA-1 |
| chr2_4 | exon | 38636141 | 38636701 | 38636141 | ID=Msa0327140-mRNA-1:exon:11584;Parent=Msa0327140-mRNA-1 |
| chr2_4 | exon | 38636747 | 38636897 | 38636747 | ID=Msa0327140-mRNA-1:exon:11585;Parent=Msa0327140-mRNA-1 |
| chr2_4 | CDS | 38626221 | 38626225 | 38626221 | ID=Msa0327140-mRNA-1:cds;Parent=Msa0327140-mRNA-1 |
| chr2_4 | CDS | 38626522 | 38626770 | 38626522 | ID=Msa0327140-mRNA-1:cds;Parent=Msa0327140-mRNA-1 |
| chr2_4 | CDS | 38627918 | 38627984 | 38627918 | ID=Msa0327140-mRNA-1:cds;Parent=Msa0327140-mRNA-1 |
| chr2_4 | three_prime_UTR | 38627985 | 38628675 | 38627985 | ID=Msa0327140-mRNA-1:three_prime_utr;Parent=Msa0327140-mRNA-1 |
| chr2_4 | three_prime_UTR | 38629537 | 38629743 | 38629537 | ID=Msa0327140-mRNA-1:three_prime_utr;Parent=Msa0327140-mRNA-1 |
| chr2_4 | three_prime_UTR | 38630746 | 38631652 | 38630746 | ID=Msa0327140-mRNA-1:three_prime_utr;Parent=Msa0327140-mRNA-1 |
| chr2_4 | three_prime_UTR | 38632672 | 38635189 | 38632672 | ID=Msa0327140-mRNA-1:three_prime_utr;Parent=Msa0327140-mRNA-1 |
| chr2_4 | three_prime_UTR | 38636141 | 38636701 | 38636141 | ID=Msa0327140-mRNA-1:three_prime_utr;Parent=Msa0327140-mRNA-1 |
| chr2_4 | three_prime_UTR | 38636747 | 38636897 | 38636747 | ID=Msa0327140-mRNA-1:three_prime_utr;Parent=Msa0327140-mRNA-1 |
| Gene Sequence |
>Msa0327140 ATGGGGTGGTTTAAGTTGACGTTTTTGGGGGAGTATGTTGTTCCTGCTGAAAGTCAAGATGTTAATGGAGAGGTTATCGACAATAGTTATGACAAGAAAGGTGAAGGGGCGAATCAAGTTAACAACGATCAATTTGATCCTTTTGTGGTTGATAATATTGAACAAGAATTTATAGCCGTTGATTCTGATGAGAATGTCGAAGATGTGGAAGTGGTCGATCATCATAATTTTGAGAAACCCGTTACAATATCCATGGGATTCTCTATTAACTCTCAAGATTCGCTTTTCTGTTGCTTAATTCTGAGTGTTACATTTATTTAA |
| Protein sequence |
>Msa0327140 MGWFKLTFLGEYVVPAESQDVNGEVIDNSYDKKGEGANQVNNDQFDPFVVDNIEQEFIAVDSDENVEDVEVVDHHNFEKPVTISMGFSINSQDSLFCCLILSVTFI* |
