AlfalfaGEDB | Msa1418960

M. sativa cultivar zhongmu-4 / Msa1418960

KEGG: K18667
GO: -
PFAM: PF07744.16

Nr:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
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Swissprot:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
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Trembl:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa1418960	A0A0C3UJQ2	83.105	438	42	1	1	438	1	406	0.0	672
Msa1418960	A0A0C3UJQ2	94.600	926	12	1	482	1369	369	1294	0.0	1816

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TFs/TRs:

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Gene ID	Type	Classification
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Protein Kinases:

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Gene ID	Type	Classification
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Network:

Co-expression Network

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Gene1	Gene2	correlation coefficient	p_value	FDR
Msa0004060	Msa1418960	-0.824308	8.658924e-54	-8.615850e-47
Msa0006880	Msa1418960	-0.855308	6.885372e-62	-8.615850e-47
Msa0007850	Msa1418960	-0.817817	2.708521e-52	-8.615850e-47
Msa0008840	Msa1418960	-0.827170	1.813728e-54	-8.615850e-47
Msa0018040	Msa1418960	0.905664	3.379282e-80	-8.615850e-47
Msa0021090	Msa1418960	0.806230	9.099321e-50	-8.615850e-47
Msa0029260	Msa1418960	-0.802039	6.779252e-49	-8.615850e-47
Msa0031510	Msa1418960	-0.827532	1.485067e-54	-8.615850e-47
Msa0045730	Msa1418960	-0.819721	1.000740e-52	-8.615850e-47
Msa0048180	Msa1418960	-0.813097	3.041831e-51	-8.615850e-47

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PPI

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Gene1	Gene2	Type
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Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa1418960	MtrunA17_Chr1g0152641	94.600	926	12	1	482	1369	369	1294	0.0	1816
Msa1418960	MtrunA17_Chr1g0152641	83.105	438	42	1	1	438	1	406	0.0	672

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Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa1418960	AT1G27750.1	39.443	431	212	11	427	844	280	674	1.24e-79	286
Msa1418960	AT1G27750.1	37.173	382	186	7	34	401	1	342	8.38e-64	238
Msa1418960	AT1G27750.1	64.912	114	40	0	1254	1367	960	1073	5.67e-37	152

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Find 383 sgRNAs with CRISPR-Local

Find 1023 sgRNAs with CRISPR-GE

CRISPR-Local

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CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
AAAATATTACCTCCTTGACG+AGG	0.701198	tig0024656:-47670	None:intergenic
AAACTACTTTCCTTGATTCA+TGG	0.286044	tig0024656:-46041	None:intergenic
AAAGAGCTGTTTGCTTATGC+TGG	0.423377	tig0024656:+41518	Msa1418960:CDS
AAAGAGGGATCTTCTAACGA+AGG	0.656688	tig0024656:+41318	Msa1418960:CDS
AAAGCAATTAGGCAATCAGA+TGG	0.578734	tig0024656:-51707	None:intergenic
AAAGCTCGACACTGTGCACC+TGG	0.500196	tig0024656:+45883	Msa1418960:CDS
AAATAGCAGCCTTGCCCATA+TGG	0.375358	tig0024656:-51633	None:intergenic
AAATGCAAATGGGATGTGGT+TGG	0.611558	tig0024656:+46619	Msa1418960:CDS
AAATTCATGGATGTGGGACT+TGG	0.545243	tig0024656:+45925	Msa1418960:CDS
AACCAGAGTTTCCAATAGCA+AGG	0.582905	tig0024656:-46572	None:intergenic

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CRISPR-GE

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sgRNA_sequence	Strand	Position	Region	GC_content
AGAGTGATGTGTTAAAAACA+AGG	-	tig0024656:38931-38950	None:intergenic	30.0%
AGAATTTCATCTTGAGACAA+CGG	-	tig0024656:39192-39211	None:intergenic	30.0%
AGAAAATTCTGATCAAGATC+AGG	-	tig0024656:39309-39328	None:intergenic	30.0%
TACTGTAGAATTGAAAACCA+TGG	+	tig0024656:39504-39523	Msa1418960:intron	30.0%
ACTCAAAACACAGTGTTAAA+AGG	-	tig0024656:39616-39635	None:intergenic	30.0%
GAAGCTAAAATTTGTAGCTT+TGG	-	tig0024656:39838-39857	None:intergenic	30.0%
AATAATAGATAGGGATATGG+GGG	+	tig0024656:40066-40085	Msa1418960:intron	30.0%
CATATGCTCGTTAAATTGTA+AGG	+	tig0024656:40176-40195	Msa1418960:intron	30.0%
AAACAGAGAATAGTAGTGAT+AGG	+	tig0024656:40220-40239	Msa1418960:intron	30.0%
TGTGCTTTATGCAACTAATT+TGG	-	tig0024656:40591-40610	None:intergenic	30.0%

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Chromosome	Type	Strat	End	Strand	Name
tig0024656	gene	38909	52226	38909	ID=Msa1418960;Name=Msa1418960
tig0024656	mRNA	38909	52226	38909	ID=Msa1418960-mRNA-1;Parent=Msa1418960;Name=Msa1418960-mRNA-1;_AED=0.09;_eAED=0.09;_QI=70\|0.45\|0.66\|0.75\|0.90\|0.91\|12\|460\|1369
tig0024656	exon	38909	39351	38909	ID=Msa1418960-mRNA-1:exon:655;Parent=Msa1418960-mRNA-1
tig0024656	exon	39512	39607	39512	ID=Msa1418960-mRNA-1:exon:656;Parent=Msa1418960-mRNA-1
tig0024656	exon	40806	41539	40806	ID=Msa1418960-mRNA-1:exon:657;Parent=Msa1418960-mRNA-1
tig0024656	exon	43604	43702	43604	ID=Msa1418960-mRNA-1:exon:658;Parent=Msa1418960-mRNA-1
tig0024656	exon	44082	44225	44082	ID=Msa1418960-mRNA-1:exon:659;Parent=Msa1418960-mRNA-1
tig0024656	exon	44983	45244	44983	ID=Msa1418960-mRNA-1:exon:660;Parent=Msa1418960-mRNA-1
tig0024656	exon	45368	46730	45368	ID=Msa1418960-mRNA-1:exon:661;Parent=Msa1418960-mRNA-1
tig0024656	exon	47459	47682	47459	ID=Msa1418960-mRNA-1:exon:662;Parent=Msa1418960-mRNA-1
tig0024656	exon	47783	48263	47783	ID=Msa1418960-mRNA-1:exon:663;Parent=Msa1418960-mRNA-1
tig0024656	exon	48600	48681	48600	ID=Msa1418960-mRNA-1:exon:664;Parent=Msa1418960-mRNA-1
tig0024656	exon	50027	50074	50027	ID=Msa1418960-mRNA-1:exon:665;Parent=Msa1418960-mRNA-1
tig0024656	exon	51563	52226	51563	ID=Msa1418960-mRNA-1:exon:666;Parent=Msa1418960-mRNA-1
tig0024656	five_prime_UTR	38909	38978	38909	ID=Msa1418960-mRNA-1:five_prime_utr;Parent=Msa1418960-mRNA-1
tig0024656	CDS	38979	39351	38979	ID=Msa1418960-mRNA-1:cds;Parent=Msa1418960-mRNA-1
tig0024656	CDS	39512	39607	39512	ID=Msa1418960-mRNA-1:cds;Parent=Msa1418960-mRNA-1
tig0024656	CDS	40806	41539	40806	ID=Msa1418960-mRNA-1:cds;Parent=Msa1418960-mRNA-1
tig0024656	CDS	43604	43702	43604	ID=Msa1418960-mRNA-1:cds;Parent=Msa1418960-mRNA-1
tig0024656	CDS	44082	44225	44082	ID=Msa1418960-mRNA-1:cds;Parent=Msa1418960-mRNA-1
tig0024656	CDS	44983	45244	44983	ID=Msa1418960-mRNA-1:cds;Parent=Msa1418960-mRNA-1
tig0024656	CDS	45368	46730	45368	ID=Msa1418960-mRNA-1:cds;Parent=Msa1418960-mRNA-1
tig0024656	CDS	47459	47682	47459	ID=Msa1418960-mRNA-1:cds;Parent=Msa1418960-mRNA-1
tig0024656	CDS	47783	48263	47783	ID=Msa1418960-mRNA-1:cds;Parent=Msa1418960-mRNA-1
tig0024656	CDS	48600	48681	48600	ID=Msa1418960-mRNA-1:cds;Parent=Msa1418960-mRNA-1
tig0024656	CDS	50027	50074	50027	ID=Msa1418960-mRNA-1:cds;Parent=Msa1418960-mRNA-1
tig0024656	CDS	51563	51766	51563	ID=Msa1418960-mRNA-1:cds;Parent=Msa1418960-mRNA-1
tig0024656	three_prime_UTR	51767	52226	51767	ID=Msa1418960-mRNA-1:three_prime_utr;Parent=Msa1418960-mRNA-1

Gene Sequence

>Msa1418960

ATGGAGGGGGAGAATCTTCTTAACACAACAAAACTCAAACGACAACATACAGAAACCACCGTAACAAACTCACCACTCTCCACCACCACCACCACTGCCATGACCTCCGTCGAACATCCTCTAAAAAAGCGAAAGCTCTACGATTCTCCACCGTCGCAATCGCCACAACCGGAAACTAGCTCTCACTTTCTGCAAACCCTACCTACTCCGCCGTTGTCTCAAGATGAAATTCTCGCGAAACGCAGAAACAAAGACGCAATTCGAAACCTCTATGAAGGTTACAAGCGAATTAAACGTTGCATCCTTCAGAAACAATCTTCCTCCATACCTGATCTTGATCAGAATTTTCTTGCTCTCATCGCATCTTCGAGAGAATTGAAAACCATGGAGGAATCGTTTGTGCATGGAATTTCACTAGGTTCTGTTGTTTGTTTGCCTCAAAATGTAGTTTCTGTAATTGATTGCTGTGGTTGTACGAGTGTACAAAGAATTGTGGCCGATTTAATTCCTCGATATGCTGGTCACTGTCCAACAGCTTTGGAAGCCGCATCCAAAGTCATTATCAATATGCATAACTGGAGTTTGGCATTGACTAGTAAGGAAGAGGATTCCAATGGTATTGCATTTGAAACTGCTAAAGCATGTATTTTCGGATTGGCTGATATTTGCTGCATTGCTTCTTCAGTAGCACCAACATCAGCTGTAATTAGAGGAATTCGCTCAACAGTTTTTCAAAATGTGCTCACCTTTTTTGTTGCCTTATTTGAGGGAAATGATGTCTTAAAGATGATTGACAAGAATTTCCTGAATATGCAAGATAATCCCGAGGTCTTTTCTGAATTGAAAGAAAAGGTTTTAGATGAAGATGATTCTTCATTGACTAAATTGTCTAAGTTCCGTGCATTATGTATACTTAGGGTATTCTTCTCTTGTCCAAAAGAATTGCTTGCAGCTTGTTTGGAACTCTTAGGCTCCACCACAAAAGAGGGATCTTCTAACGAAGGACAGCGTTTTCTGAGCATGGTGACTAGCATGCTTAATTATGATGAAGCAGTTCACCTTTTGGGTAGAGCAAATGATGGACCTAAATCATGTAACGATTCTATTGAGGAAGGCATCAAAGAGATTGAGGTTGGCGAGAAAGCCGTCACCAATGACAACCATATCTCCGATGCGATTCAAAAGAGCTGTTTGCTTATGCTGTGCAATGGCGGAGATCGGCAAGGTGGTATTGAAAACAATGAGAGGAACAAGAATATATGGGATTCAAATGATCCAGGAAAAAAGGAGCTCATATCGAAGGAGGAGGGAGTGAATGAGAAAGAGAGTCCAGTTGTAGATAATGAGCTCAAGGAGGATGCTGATTTGGAAGTGAATGGCCCAATTAATCAGTGTGTTTTGGCTGCACTTGAAGTGGAAGGAGCAAGTGAGAAGGCGAAGAGGATGGTTCTTAACAAGGACCCCTCACTGCGGAAGTGGACTCTGCGCCGATGTAAGAAGTTGCTTGACTCGCTCACTAATGCTTCACCGGAAACTACATCAGTATTGCAAGGAATTCTTGGAATGTTCGCTCAACAGACAGAGTTAGAAGACTGTCAAGTAGATAGTGATGAAGATAAGTCCGATTCTTCAATCTTCACGAATAGAAACTATGTGATTCCTAGGATCTCCGAGGAACTTGAAAATATTGGTGAAAGATCACAAAAAGGTTCGCACTTTGATAATGGAGGGGTATCAAGGTCTGTGGGCGTTGAGAAAGGTGATATACCCCATGTTAGATGTTCCACACCTAGGGACTCTGTAAGCCATCAGATGTTCTCACCTACAGTTAGAACAGCAGTAGACTTTAGGAGTAATTCATTTGATGGTAGAAATGAATTCCCAAATGTTGAGAAAAATCAAGTTTCAAATATAAATTTCAATTCACCTCTGTCGAGGTCCTCTAGTGGAGCTGTCAGCAATGTTCTGGCTTCTCCCAATCATCGTTTTATGTCACCAACTTCTTTAACCAAAAGTCAAATTGTTTTGTGCTTTGACGGGGATCCTGCTGCTGTAGATATTGTTGCTGCTTCTAGGCAGCTATGGGTAGGTTGTGTGGCACCTGACATGCCTGAAAGCCATATTAGGTTTCAAATAGAGAGGTTTGGTCCTATTGAAAAGTTTATATTCTTCCCATCGAATGGATTTGCTTTAGTTGAGTACAGAAGGATAATGGACGCAATAAAAGCTCGACACTGTGCACCTGGAAATTTTCCTTGCCGTGTAAAATTCATGGATGTGGGACTTGGTAGTAGGGGTGCAGTGAATGGTGTTGCAGTTGGCTCTAGTTCTCATATTTATGTTGGAAATGTTTCCAGTCAATGGGCCAAGGATGAGATTCTCCATGAATCAAGGAAAGTAGTTTATAAGGGTCCTCTCGCTGTGATTGATCTTAGCTTTGAGTGTGCGTTGCTTATGGAGTTTGAAAGCCCTGAAGAAGCTGCCTCCGTCATGTTGCATCTGAGACAGCTCCGAAGAGAAAGAAGTAGTTATAGCCCACATTTTGGTCCAGGAACAGTTAATGTTGTAAGTGGGCATGGTTATATGGATGGTGCAAGACCTTTGCCCGCCCCTGCTCACCTGGACCTCAAGGTCAGCAACTCTGCTGGATCACCTCATGCTCGAGCTTTACATGGGAGCCCTGCCGACAGCAGTCGAACAAGAATGTCTCACTTGTGTAACATACTAGCTTCATTGCGTGCAAAGTATAATATTAATCAAAACATAGGTCTCCATGATAATTATATGACTGGAAATAGTTGCGCTTCTTCCACGCGTGAGGAAGATGCAGTGCCATCCAACACTCTGTGGATAACAATTCCTCATAGTAGCTCCCAGTTCCTCACAGATGATGAGCTAATGAGTATTTGCAACCTTGCTATTGGAAACTCTGGTTCCATTGCACGGTTGACACAAGCAAAAATGCAAATGGGATGTGGTTGGTTTGTTGAATGTAGTAATGTTGACGGAGCAGTTACAGTCTTAAAGAATCTGCGTGGTTGTCCAGGACTGTTTTTCCAAATAGAATTCAGCAATCCAGGGAATCAGAATGCTGTTCCATTTGCAATTAAACCTGAAAACCGTGCTATGGAGCTTGTCTCCCCCAGAATGAATGCTGAGAATCGTAGTAGTGGAGGACATGGCGCACCTCTGTCTCAATCAAACTGGCACTTCCCTGATTCAAGAGAGATGCCAGAAGTTGGAGGAAGGAAACCTGATGGTTATGATCATTTATCACTGGATCCTCGTCAAGGAGGAAATGTTCCACATGCATACTCTGGAGCACATGGACCTTCCATTCCACCACCACAACAAATTCAGTCATCTCCTTTCACCCGCCCTGTTTATGTTCCTCCAAATGGCCAATGGGATCCCCATGGAATAAACAATCAGTTACCTGTGAACCAATACCAAACTGTTGTAATGCCAAATAATTTTAATGGCAGTCCTTTTATACCTGTTTCAGTAACTCCACTTGCTCAAATACAGGGAACTCCAATGCAACCGTATAACCAGCAGATTCCTCCCTCAATTGTACCACCACCCTTATCATCCTTGCCACCTCCTCAGCCTGAAATGCCACCTCCTCATCCACATCCTCCTTCTCAGCCACCTCTACCTGAGACACAACCCCCCTTGGTTCCTCCACCTCCTCCACCTGGTTCTCCTCCACCTCCTCCTCCTCCGCTGCCTGTCCAAGAACCAGTCAATATGGAATGTTCGGGGCAGCCGCTGCAATGGCCCACCAAATTAGACATGACTAAACGCACAGATTTTAAACATGTGCAATCAACATTTGCTGCTACTCCATCTCATAGAAGGGAAGTGTGCCGTTTAATCCCATCTTCCACAAGTGAAGACAGAAGGTTTCAGGATTTCGTATCATATTTAAAACAGAGGGATTGTGCTGGAGTTATAAAAGTCCCAGCTTCAAAATCCATATGGGCAAGGCTGCTATTTTTACTTCCTCATTCACTTGAAATGTGCTCGTTGCTTTCTATTGCACCCGATCCATCTGATTGCCTAATTGCTTTGGTTTTACCCAAAGAAACAAACTCTGACTGGTTATAA

Protein sequence

>Msa1418960

MEGENLLNTTKLKRQHTETTVTNSPLSTTTTTAMTSVEHPLKKRKLYDSPPSQSPQPETSSHFLQTLPTPPLSQDEILAKRRNKDAIRNLYEGYKRIKRCILQKQSSSIPDLDQNFLALIASSRELKTMEESFVHGISLGSVVCLPQNVVSVIDCCGCTSVQRIVADLIPRYAGHCPTALEAASKVIINMHNWSLALTSKEEDSNGIAFETAKACIFGLADICCIASSVAPTSAVIRGIRSTVFQNVLTFFVALFEGNDVLKMIDKNFLNMQDNPEVFSELKEKVLDEDDSSLTKLSKFRALCILRVFFSCPKELLAACLELLGSTTKEGSSNEGQRFLSMVTSMLNYDEAVHLLGRANDGPKSCNDSIEEGIKEIEVGEKAVTNDNHISDAIQKSCLLMLCNGGDRQGGIENNERNKNIWDSNDPGKKELISKEEGVNEKESPVVDNELKEDADLEVNGPINQCVLAALEVEGASEKAKRMVLNKDPSLRKWTLRRCKKLLDSLTNASPETTSVLQGILGMFAQQTELEDCQVDSDEDKSDSSIFTNRNYVIPRISEELENIGERSQKGSHFDNGGVSRSVGVEKGDIPHVRCSTPRDSVSHQMFSPTVRTAVDFRSNSFDGRNEFPNVEKNQVSNINFNSPLSRSSSGAVSNVLASPNHRFMSPTSLTKSQIVLCFDGDPAAVDIVAASRQLWVGCVAPDMPESHIRFQIERFGPIEKFIFFPSNGFALVEYRRIMDAIKARHCAPGNFPCRVKFMDVGLGSRGAVNGVAVGSSSHIYVGNVSSQWAKDEILHESRKVVYKGPLAVIDLSFECALLMEFESPEEAASVMLHLRQLRRERSSYSPHFGPGTVNVVSGHGYMDGARPLPAPAHLDLKVSNSAGSPHARALHGSPADSSRTRMSHLCNILASLRAKYNINQNIGLHDNYMTGNSCASSTREEDAVPSNTLWITIPHSSSQFLTDDELMSICNLAIGNSGSIARLTQAKMQMGCGWFVECSNVDGAVTVLKNLRGCPGLFFQIEFSNPGNQNAVPFAIKPENRAMELVSPRMNAENRSSGGHGAPLSQSNWHFPDSREMPEVGGRKPDGYDHLSLDPRQGGNVPHAYSGAHGPSIPPPQQIQSSPFTRPVYVPPNGQWDPHGINNQLPVNQYQTVVMPNNFNGSPFIPVSVTPLAQIQGTPMQPYNQQIPPSIVPPPLSSLPPPQPEMPPPHPHPPSQPPLPETQPPLVPPPPPPGSPPPPPPPLPVQEPVNMECSGQPLQWPTKLDMTKRTDFKHVQSTFAATPSHRREVCRLIPSSTSEDRRFQDFVSYLKQRDCAGVIKVPASKSIWARLLFLLPHSLEMCSLLSIAPDPSDCLIALVLPKETNSDWL*