AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa0682160

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0682160 MtrunA17_Chr4g0028271 94.979 478 24 0 19 496 21 498 0.0 954
Msa0682160 MtrunA17_Chr4g0028261 81.250 496 89 3 1 493 23 517 0.0 838
Msa0682160 MtrunA17_Chr4g0028281 73.992 496 125 2 1 494 1 494 0.0 773
Msa0682160 MtrunA17_Chr2g0327401 64.718 496 165 6 7 497 13 503 0.0 671
Msa0682160 MtrunA17_Chr1g0199171 61.277 501 171 6 3 496 7 491 0.0 619
Msa0682160 MtrunA17_Chr4g0028241 63.750 480 160 7 19 493 21 491 0.0 617
Msa0682160 MtrunA17_Chr2g0327381 57.249 538 170 9 9 497 16 542 0.0 600
Msa0682160 MtrunA17_Chr1g0171411 60.484 496 181 4 7 498 3 487 0.0 598
Msa0682160 MtrunA17_Chr2g0327371 54.851 505 158 7 34 493 35 514 0.0 541
Msa0682160 MtrunA17_Chr5g0447441 33.005 406 255 7 91 489 37 432 7.69e-70 230
Msa0682160 MtrunA17_Chr4g0044051 32.020 406 259 7 91 489 41 436 1.26e-65 219
Msa0682160 MtrunA17_Chr3g0118851 29.767 430 238 14 95 493 92 488 5.82e-52 183
Msa0682160 MtrunA17_Chr3g0118841 30.048 416 254 12 95 493 93 488 9.54e-52 183
Msa0682160 MtrunA17_Chr3g0090331 28.163 490 314 12 27 491 10 486 2.08e-51 182
Msa0682160 MtrunA17_Chr7g0249431 28.355 462 258 12 95 491 42 495 4.91e-51 181
Msa0682160 MtrunA17_Chr8g0338461 28.665 457 259 11 95 492 45 493 3.42e-50 179
Msa0682160 MtrunA17_Chr3g0094811 30.303 429 245 18 95 491 44 450 2.51e-49 175
Msa0682160 MtrunA17_Chr1g0153881 29.556 450 270 13 67 493 70 495 2.97e-48 174
Msa0682160 MtrunA17_Chr3g0118881 29.930 431 258 15 88 493 85 496 4.18e-48 173
Msa0682160 MtrunA17_Chr4g0068841 28.276 435 276 12 88 492 46 474 4.82e-48 172
Msa0682160 MtrunA17_Chr2g0309541 29.977 427 249 13 95 491 53 459 5.16e-48 172
Msa0682160 MtrunA17_Chr3g0118831 31.415 417 217 17 95 493 92 457 7.45e-48 172
Msa0682160 MtrunA17_Chr2g0309521 30.549 419 255 13 95 491 54 458 1.45e-47 171
Msa0682160 MtrunA17_Chr7g0234051 28.355 462 253 11 95 491 44 492 2.64e-47 171
Msa0682160 MtrunA17_Chr5g0431301 29.505 444 264 13 88 497 37 465 5.54e-47 169
Msa0682160 MtrunA17_Chr2g0309511 30.266 413 264 12 95 491 54 458 1.39e-46 168
Msa0682160 MtrunA17_Chr2g0304931 28.959 442 252 18 86 491 43 458 1.68e-46 168
Msa0682160 MtrunA17_Chr8g0385871 27.962 422 265 12 95 489 47 456 1.12e-45 166
Msa0682160 MtrunA17_Chr3g0093971 29.953 424 257 12 97 493 43 453 1.24e-45 165
Msa0682160 MtrunA17_Chr4g0047861 28.235 425 273 12 92 491 64 481 3.31e-45 165
Msa0682160 MtrunA17_Chr2g0318911 29.002 431 256 13 95 496 55 464 4.24e-45 164
Msa0682160 MtrunA17_Chr3g0118891 30.900 411 225 16 95 493 92 455 4.85e-45 164
Msa0682160 MtrunA17_Chr3g0131731 29.108 426 256 15 97 491 47 457 7.97e-45 163
Msa0682160 MtrunA17_Chr1g0199991 28.538 424 256 13 95 490 47 451 1.56e-44 162
Msa0682160 MtrunA17_Chr3g0126031 28.878 419 268 12 95 491 42 452 1.08e-43 160
Msa0682160 MtrunA17_Chr4g0020241 27.442 430 262 13 87 491 79 483 2.56e-43 160
Msa0682160 MtrunA17_Chr3g0133011 27.570 428 251 12 107 492 84 494 2.85e-43 160
Msa0682160 MtrunA17_Chr4g0009711 27.505 469 273 15 62 489 23 465 4.52e-43 159
Msa0682160 MtrunA17_Chr1g0153941 27.815 453 253 14 88 493 85 510 6.09e-43 159
Msa0682160 MtrunA17_Chr3g0126051 29.302 430 267 14 88 491 41 459 6.56e-43 158
Msa0682160 MtrunA17_Chr7g0221551 28.241 432 259 17 92 491 41 453 7.53e-43 158
Msa0682160 MtrunA17_Chr2g0285371 26.777 422 273 9 97 489 50 464 2.35e-42 157
Msa0682160 MtrunA17_Chr7g0219051 28.966 435 244 15 97 491 51 460 3.78e-42 156
Msa0682160 MtrunA17_Chr4g0009681 27.716 451 287 13 65 488 23 461 4.24e-42 156
Msa0682160 MtrunA17_Chr2g0285351 27.483 433 259 11 96 489 48 464 5.05e-42 156
Msa0682160 MtrunA17_Chr4g0009661 29.257 417 239 13 109 489 69 465 7.60e-42 155
Msa0682160 MtrunA17_Chr3g0118861 29.017 417 244 14 92 493 87 466 4.06e-41 153
Msa0682160 MtrunA17_Chr2g0285381 26.730 419 270 10 97 489 53 460 1.32e-40 152
Msa0682160 MtrunA17_Chr1g0199971 27.650 434 259 15 88 489 40 450 1.73e-40 151
Msa0682160 MtrunA17_Chr4g0009651 28.780 410 250 13 109 489 69 465 2.38e-40 151
Msa0682160 MtrunA17_Chr4g0038311 30.000 430 246 16 95 489 48 457 2.43e-40 151
Msa0682160 MtrunA17_Chr4g0068861 29.659 381 216 13 88 429 92 459 2.73e-39 148
Msa0682160 MtrunA17_Chr4g0009701 28.571 434 245 15 97 489 56 465 4.20e-39 148
Msa0682160 MtrunA17_Chr2g0281621 27.123 424 271 11 95 491 49 461 9.59e-39 147
Msa0682160 MtrunA17_Chr4g0009671 25.882 425 243 12 109 489 34 430 2.67e-37 142
Msa0682160 MtrunA17_Chr4g0027731 25.688 436 272 11 91 487 29 451 1.91e-34 134
Msa0682160 MtrunA17_Chr1g0153931 39.548 177 99 5 88 258 83 257 2.83e-34 130
Msa0682160 MtrunA17_Chr1g0165811 34.818 247 141 10 86 329 36 265 5.69e-34 129
Msa0682160 MtrunA17_Chr7g0234041 35.686 255 128 7 95 342 41 266 7.56e-34 133
Msa0682160 MtrunA17_Chr4g0009641 26.024 415 256 12 97 489 56 441 2.40e-32 128
Msa0682160 MtrunA17_Chr2g0320571 26.906 446 263 15 91 486 29 461 4.87e-27 113
Msa0682160 MtrunA17_Chr2g0279371 31.973 147 94 1 103 243 35 181 2.97e-26 105
Msa0682160 MtrunA17_Chr2g0281641 24.427 393 247 10 133 491 7 383 3.38e-26 110
Msa0682160 MtrunA17_Chr1g0204191 23.434 431 243 17 97 491 107 486 1.80e-21 97.4
Msa0682160 MtrunA17_Chr4g0009721 22.556 399 242 12 109 489 65 414 5.41e-21 95.1
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0682160 AT3G45010.1 62.651 498 172 5 6 494 9 501 0.0 652
Msa0682160 AT3G10410.1 62.272 493 176 6 7 493 8 496 0.0 643
Msa0682160 AT5G22980.1 61.111 504 181 7 4 498 3 500 0.0 628
Msa0682160 AT1G15000.1 31.941 407 257 9 91 491 31 423 1.83e-66 221
Msa0682160 AT5G22960.1 58.602 186 57 4 70 254 8 174 1.47e-65 210
Msa0682160 AT3G25420.1 29.158 463 247 11 95 491 44 491 4.17e-57 197
Msa0682160 AT4G12910.1 29.412 459 251 11 95 492 49 495 2.83e-55 192
Msa0682160 AT3G52000.1 29.594 419 264 9 88 490 71 474 2.93e-52 184
Msa0682160 AT3G52010.1 29.302 430 255 12 88 490 72 479 2.96e-51 182
Msa0682160 AT3G52020.1 30.024 423 260 16 88 490 87 493 1.46e-49 177
Msa0682160 AT3G12203.3 28.434 415 253 14 96 491 24 413 1.75e-49 175
Msa0682160 AT3G17180.1 30.698 430 258 16 93 491 51 471 2.13e-49 176
Msa0682160 AT5G08260.1 32.184 435 238 18 95 489 53 470 2.67e-49 176
Msa0682160 AT2G22920.2 28.604 444 275 16 66 492 15 433 3.60e-49 175
Msa0682160 AT1G73300.1 27.230 426 273 12 81 491 35 438 4.21e-49 175
Msa0682160 AT1G73300.2 27.230 426 273 12 81 491 35 438 4.21e-49 175
Msa0682160 AT3G12203.1 28.365 416 254 14 96 492 45 435 4.57e-49 174
Msa0682160 AT3G12203.2 28.928 401 241 14 110 491 1 376 1.96e-48 171
Msa0682160 AT1G73280.1 26.813 455 281 14 59 492 16 439 1.03e-47 171
Msa0682160 AT2G23010.1 26.818 440 289 11 65 491 15 434 1.43e-47 171
Msa0682160 AT5G23210.1 31.435 439 243 17 95 489 65 489 1.96e-47 172
Msa0682160 AT2G35770.1 27.465 426 267 9 88 489 45 452 3.14e-47 170
Msa0682160 AT4G12910.2 29.620 395 211 9 95 429 49 436 3.30e-47 170
Msa0682160 AT2G23010.2 26.591 440 290 11 65 491 15 434 5.77e-47 169
Msa0682160 AT1G73270.2 26.585 410 268 9 96 491 48 438 5.89e-47 169
Msa0682160 AT1G73270.1 26.521 411 269 9 96 492 48 439 7.64e-47 169
Msa0682160 AT3G63470.1 29.274 427 242 17 95 490 95 492 1.91e-46 169
Msa0682160 AT5G36180.1 27.427 412 262 12 96 491 48 438 2.12e-46 167
Msa0682160 AT3G07990.1 27.725 422 274 12 88 489 41 451 2.53e-46 167
Msa0682160 AT1G73310.1 26.353 425 278 11 81 491 35 438 2.79e-46 167
Msa0682160 AT2G22980.1 27.211 441 268 15 68 489 19 425 3.47e-46 167
Msa0682160 AT4G15100.1 29.186 442 261 16 95 498 58 485 3.50e-46 168
Msa0682160 AT4G30810.1 27.725 422 271 10 93 492 50 459 8.32e-46 167
Msa0682160 AT2G22970.1 26.984 441 277 16 68 491 18 430 2.42e-45 164
Msa0682160 AT1G73290.1 26.406 409 270 8 96 492 47 436 3.22e-45 164
Msa0682160 AT2G22920.1 30.026 383 233 13 66 432 15 378 5.37e-45 163
Msa0682160 AT2G24000.1 29.009 424 262 14 97 492 46 458 7.25e-45 164
Msa0682160 AT3G10450.1 27.187 423 269 14 86 492 36 435 1.14e-44 163
Msa0682160 AT3G10450.2 26.829 410 263 13 99 492 4 392 1.38e-43 159
Msa0682160 AT3G12220.1 28.846 416 255 17 96 493 42 434 2.37e-43 159
Msa0682160 AT3G12240.1 28.019 414 259 15 96 492 43 434 3.56e-43 159
Msa0682160 AT2G12480.2 28.643 398 265 8 97 491 47 428 5.08e-43 158
Msa0682160 AT2G12480.4 29.104 402 259 10 97 491 47 429 6.22e-43 158
Msa0682160 AT3G02110.1 27.982 436 252 16 97 491 53 467 1.07e-42 158
Msa0682160 AT1G73290.3 27.874 348 227 5 96 432 47 381 1.19e-42 156
Msa0682160 AT3G12220.2 29.280 403 244 17 109 493 25 404 1.57e-42 156
Msa0682160 AT2G22980.2 28.198 383 229 12 68 432 19 373 1.87e-42 156
Msa0682160 AT4G30610.1 29.070 430 259 17 93 491 44 458 2.30e-42 157
Msa0682160 AT2G24010.1 28.986 414 263 12 97 491 46 447 2.44e-42 157
Msa0682160 AT2G35780.1 27.314 443 271 12 76 489 25 445 2.66e-42 157
Msa0682160 AT2G22980.4 28.571 385 225 13 68 432 19 373 3.04e-42 156
Msa0682160 AT2G12480.1 28.643 398 264 9 97 491 47 427 4.45e-42 155
Msa0682160 AT1G43780.1 30.140 428 252 14 97 491 53 466 7.53e-42 156
Msa0682160 AT5G23210.3 40.887 203 115 4 95 292 65 267 8.26e-42 155
Msa0682160 AT1G73310.2 27.123 365 238 8 81 432 35 384 1.27e-41 154
Msa0682160 AT1G28110.1 27.419 434 256 17 95 492 45 455 1.82e-41 154
Msa0682160 AT1G28110.2 27.419 434 256 17 95 492 45 455 1.82e-41 154
Msa0682160 AT2G22970.4 28.346 381 235 13 68 432 18 376 1.89e-41 153
Msa0682160 AT5G42230.1 28.604 437 266 11 95 498 42 465 2.40e-41 154
Msa0682160 AT5G42230.2 28.604 437 266 11 95 498 42 465 2.40e-41 154
Msa0682160 AT1G11080.2 28.798 441 251 15 95 491 64 485 3.05e-41 154
Msa0682160 AT1G11080.1 28.864 440 252 16 95 491 64 485 3.13e-41 154
Msa0682160 AT5G42240.1 29.647 425 261 13 95 491 46 460 3.17e-41 154
Msa0682160 AT2G22970.3 28.346 381 235 13 68 432 18 376 4.10e-41 153
Msa0682160 AT2G22990.1 26.376 436 266 16 81 491 25 430 4.19e-41 153
Msa0682160 AT2G23000.1 26.492 419 259 13 97 491 41 434 5.09e-41 153
Msa0682160 AT2G23000.2 26.492 419 259 13 97 491 41 434 5.09e-41 153
Msa0682160 AT1G61130.1 28.333 420 270 12 95 491 45 456 5.26e-41 153
Msa0682160 AT2G22920.4 32.268 313 182 10 68 368 18 312 9.99e-41 149
Msa0682160 AT1G73290.2 35.714 196 120 1 96 285 47 242 1.06e-40 149
Msa0682160 AT2G12480.3 28.095 420 264 9 97 491 47 453 1.28e-40 152
Msa0682160 AT3G12230.1 28.606 416 254 17 96 492 42 433 1.53e-40 151
Msa0682160 AT3G10450.4 28.453 362 227 11 86 432 36 380 1.60e-40 151
Msa0682160 AT1G33540.1 26.559 433 266 13 96 491 44 461 4.06e-40 151
Msa0682160 AT1G33540.2 26.512 430 264 13 99 491 4 418 1.43e-39 148
Msa0682160 AT2G33530.1 26.790 433 262 16 95 492 47 459 1.44e-39 149
Msa0682160 AT3G17180.2 31.063 367 219 14 93 429 51 413 1.66e-39 149
Msa0682160 AT3G10450.3 28.080 349 221 10 99 432 4 337 1.85e-39 147
Msa0682160 AT2G05850.1 26.014 419 282 12 88 489 71 478 3.60e-39 149
Msa0682160 AT5G09640.1 25.780 481 267 17 66 491 17 462 1.03e-38 147
Msa0682160 AT3G12220.3 35.628 247 142 7 96 326 42 287 1.04e-38 145
Msa0682160 AT1G73280.2 29.560 318 203 7 59 368 16 320 3.32e-38 144
Msa0682160 AT2G22970.2 37.745 204 113 5 68 264 18 214 3.36e-38 142
Msa0682160 AT2G24010.2 27.739 429 264 13 97 491 46 462 1.05e-37 144
Msa0682160 AT2G22980.3 29.712 313 187 9 68 368 19 310 1.43e-37 141
Msa0682160 AT2G22990.4 26.617 402 242 14 81 458 25 397 3.52e-37 142
Msa0682160 AT2G22990.3 25.686 401 247 12 81 458 25 397 7.39e-37 142
Msa0682160 AT5G23210.5 30.923 401 219 17 133 489 8 394 6.38e-36 138
Msa0682160 AT5G23210.2 30.750 400 219 17 134 489 8 393 3.12e-35 136
Msa0682160 AT2G22990.5 24.598 435 266 16 81 491 25 421 1.86e-33 131
Msa0682160 AT2G24010.3 26.211 454 264 14 97 491 17 458 7.30e-33 130
Msa0682160 AT3G56540.1 40.136 147 85 3 88 232 87 232 2.80e-32 124
Msa0682160 AT2G27920.1 26.941 438 259 17 94 486 32 453 1.04e-30 124
Msa0682160 AT2G24010.4 28.940 349 195 13 97 425 17 332 3.49e-30 121
Msa0682160 AT1G73280.4 25.641 351 220 11 156 491 12 336 4.84e-30 120
Msa0682160 AT5G23210.4 41.463 164 91 4 134 292 8 171 9.84e-30 120
Msa0682160 AT5G36180.5 26.012 346 225 11 156 491 12 336 7.46e-29 117
Msa0682160 AT5G36180.3 26.012 346 225 11 156 491 12 336 7.46e-29 117
Msa0682160 AT1G73280.3 24.629 337 213 11 170 491 1 311 1.58e-25 107
Msa0682160 AT5G36180.4 25.444 338 217 10 156 475 12 332 1.34e-24 105
Msa0682160 AT5G36180.2 26.923 286 185 8 156 432 12 282 1.59e-24 104
Msa0682160 AT1G73310.3 27.148 291 178 10 156 432 12 282 3.55e-24 103
Msa0682160 AT2G27920.2 25.895 363 218 13 164 486 30 381 1.47e-23 102
Msa0682160 AT2G22990.6 23.907 343 214 13 168 491 2 316 9.22e-23 99.4
Msa0682160 AT2G22990.2 23.615 343 215 13 168 491 2 316 3.10e-22 97.8
Msa0682160 AT2G22980.5 25.714 280 168 9 168 432 2 256 4.02e-21 94.0
Msa0682160 AT2G27920.3 24.857 350 212 13 177 486 48 386 3.41e-18 87.0

Find 96 sgRNAs with CRISPR-Local

Find 211 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
GCAGGATTGTTAAATATTTA+TGG 0.106475 4_4:+43258473 Msa0682160:CDS
ATTTAATTACCTTGCGGATT+TGG 0.221438 4_4:-43255365 None:intergenic
CGTTGGTTTGGTGAAAATTT+TGG 0.249695 4_4:-43255151 None:intergenic
ACTTCTTGAGGATGGAATTA+AGG 0.263632 4_4:+43258075 Msa0682160:CDS
CCACAAAAGAGACAAGTTAT+TGG 0.264870 4_4:-43256246 None:intergenic
TTTGGGCAGGGAATTCAAAA+TGG 0.268125 4_4:+43258372 Msa0682160:intron
AAGAATGACTTCTATATTAC+TGG 0.289799 4_4:+43256935 Msa0682160:CDS
GATCCTTATCTCAATGTTAG+AGG 0.317651 4_4:-43258769 None:intergenic
ATGTTCCTGCTCTTGCATCT+AGG 0.318626 4_4:+43256978 Msa0682160:CDS
CACTTCAGATGTTGGCAAAC+TGG 0.324425 4_4:+43258666 Msa0682160:CDS
ATTCCATCCTCAAGAAGTTC+AGG 0.365252 4_4:-43258070 None:intergenic
TTTATGGCAACCTCGCAATC+TGG 0.365782 4_4:-43257460 None:intergenic
TCACCATGAATTATATTAAC+TGG 0.366105 4_4:-43255217 None:intergenic
TTAGGGATGCTTTAGGTGTT+GGG 0.366917 4_4:+43257966 Msa0682160:CDS
ATTATATTAACTGGATCCTT+TGG 0.371296 4_4:-43255208 None:intergenic
ATGACTTCATGTATAATGTT+TGG 0.371558 4_4:+43258809 Msa0682160:three_prime_UTR
CCAGTCAACCATATCACAAC+TGG 0.373577 4_4:-43256164 None:intergenic
AAGACAGTTAGGGATGCTTT+AGG 0.386288 4_4:+43257959 Msa0682160:CDS
TATGCTTGGTACTGAATTGC+TGG 0.395817 4_4:-43257370 None:intergenic
AAGTCATTCTTAACAAACTT+AGG 0.397900 4_4:-43256923 None:intergenic
TGTTCCTGCTCTTGCATCTA+GGG 0.398381 4_4:+43256979 Msa0682160:CDS
TGATTTGTACGACTTCTTGC+TGG 0.399728 4_4:+43256768 Msa0682160:CDS
TTCTAAACAAGAAGACAGTT+AGG 0.414633 4_4:+43257948 Msa0682160:CDS
AACATCTGAAGTGCAGCTTT+TGG 0.416607 4_4:-43258656 None:intergenic
TACATTAACGTTACCGGCAA+TGG 0.417475 4_4:-43257771 None:intergenic
GGATGCATGGGAACTTGAAT+AGG 0.421333 4_4:+43258687 Msa0682160:CDS
GATCCAGTTAATATAATTCA+TGG 0.424315 4_4:+43255214 Msa0682160:CDS
TATCTGTTCACATTGTTGTA+AGG 0.425655 4_4:-43257732 None:intergenic
GGTGAATGGGGCAGGACATA+TGG 0.430010 4_4:+43258619 Msa0682160:intron
AGTACTTACATTAACGTTAC+CGG 0.431784 4_4:-43257777 None:intergenic
AGTCATTCTTAACAAACTTA+GGG 0.434933 4_4:-43256922 None:intergenic
ATTAAGGTTCTTATATATGC+AGG 0.439088 4_4:+43258091 Msa0682160:CDS
AGGGTTCACCATGGAAATAA+AGG 0.441831 4_4:+43256998 Msa0682160:CDS
GTTAGGGATGCTTTAGGTGT+TGG 0.454819 4_4:+43257965 Msa0682160:CDS
GACCTGACCACTTCATTGCA+TGG 0.456662 4_4:-43258397 None:intergenic
ATAATGCAATGTTGCAAGAT+TGG 0.457321 4_4:+43258023 Msa0682160:CDS
AGGATGCCCCAGTTGTGATA+TGG 0.462648 4_4:+43256156 Msa0682160:CDS
TAGCTAAAACCTGTCCCAAT+TGG 0.473861 4_4:-43256697 None:intergenic
GATATTCCACATGATGAAAC+TGG 0.479658 4_4:+43256736 Msa0682160:CDS
TTATGGCAACCTCGCAATCT+GGG 0.484542 4_4:-43257459 None:intergenic
GTCCATGCAATGAAGTGGTC+AGG 0.489340 4_4:+43258395 Msa0682160:CDS
TGACCGAGATCTTCAATAGA+AGG 0.490638 4_4:-43255313 None:intergenic
GGCAGGACATATGGTCCCTA+TGG 0.494483 4_4:+43258628 Msa0682160:CDS
TTGGGCAGGGAATTCAAAAT+GGG 0.506482 4_4:+43258373 Msa0682160:intron
ATGCAAACTGAGAGTATGCT+TGG 0.507916 4_4:-43257384 None:intergenic
ATTCCTGAACTTCTTGAGGA+TGG 0.511069 4_4:+43258067 Msa0682160:CDS
GTGATGTGTAACAGGTGAAT+GGG 0.514606 4_4:+43258606 Msa0682160:intron
AAGCTTTAACTTATTGCCAA+AGG 0.518571 4_4:+43255192 Msa0682160:CDS
TTTGTGGAATGATTATGGTT+GGG 0.519215 4_4:+43256262 Msa0682160:CDS
ATATGGTTGACTGGAGGTCC+AGG 0.522115 4_4:+43256173 Msa0682160:CDS
TTGGTACTGAATTGCTGGAT+TGG 0.523254 4_4:-43257365 None:intergenic
ACGAGGAAGCGAATAGTATC+CGG 0.523737 4_4:-43255341 None:intergenic
TTTGAATTGGCAGAGAGTAA+AGG 0.527254 4_4:+43257689 Msa0682160:intron
GTTGACCAACCAATTGGGAC+AGG 0.528579 4_4:+43256688 Msa0682160:CDS
CATCCTCTAACATTGAGATA+AGG 0.530865 4_4:+43258766 Msa0682160:three_prime_UTR
GCTAGTTCACTGCTGCAACC+TGG 0.533445 4_4:-43256191 None:intergenic
GGATATTCCTGAACTTCTTG+AGG 0.534859 4_4:+43258063 Msa0682160:CDS
AAAAGCTGCACTTCAGATGT+TGG 0.535873 4_4:+43258658 Msa0682160:CDS
TAGGGATGCTTTAGGTGTTG+GGG 0.537401 4_4:+43257967 Msa0682160:CDS
TGTGATGTGTAACAGGTGAA+TGG 0.544527 4_4:+43258605 Msa0682160:intron
GAAGATCTCGGTCACCATGC+CGG 0.547294 4_4:+43255322 Msa0682160:CDS
CCAGTTGTGATATGGTTGAC+TGG 0.551036 4_4:+43256164 Msa0682160:CDS
ATTACTGGAGAATCATATGC+TGG 0.551568 4_4:+43256950 Msa0682160:CDS
TTAAGGTTCTTATATATGCA+GGG 0.553185 4_4:+43258092 Msa0682160:CDS
GAATTGGCAGAGAGTAAAGG+TGG 0.560172 4_4:+43257692 Msa0682160:intron
TCTAAACAAGAAGACAGTTA+GGG 0.561300 4_4:+43257949 Msa0682160:CDS
GACATAAGAAAGAAATGTCA+AGG 0.567445 4_4:+43257890 Msa0682160:CDS
TTGGATGAGAAACTTTGAAG+TGG 0.571105 4_4:+43258042 Msa0682160:CDS
CAGTCAACCATATCACAACT+GGG 0.574498 4_4:-43256163 None:intergenic
ATCTTCAATAGAAGGACCAT+CGG 0.575473 4_4:-43255305 None:intergenic
ATGTTGGCAAACTGGATGCA+TGG 0.577839 4_4:+43258674 Msa0682160:CDS
CTAACACCAGTTTCATCATG+TGG 0.588201 4_4:-43256742 None:intergenic
GATGCGAGAAGTTGCGTATG+AGG 0.589321 4_4:-43255101 None:intergenic
GTGTAACAGGTGAATGGGGC+AGG 0.590576 4_4:+43258611 Msa0682160:intron
TACCTTGCGGATTTGGAACG+AGG 0.592095 4_4:-43255358 None:intergenic
TAAAACCTGTCCCAATTGGT+TGG 0.592919 4_4:-43256693 None:intergenic
TGATAACAAAATGATTACAA+AGG 0.594810 4_4:+43257408 Msa0682160:CDS
GGTCCTTCTATTGAAGATCT+CGG 0.595179 4_4:+43255310 Msa0682160:CDS
ACTTCATGTATAATGTTTGG+AGG 0.595677 4_4:+43258812 Msa0682160:three_prime_UTR
CAAGTTGATCCCAGATTGCG+AGG 0.597604 4_4:+43257450 Msa0682160:CDS
AGGGATTACTATAAACCTCA+AGG 0.608711 4_4:+43257027 Msa0682160:CDS
TGTTGGCAAACTGGATGCAT+GGG 0.618606 4_4:+43258675 Msa0682160:CDS
AATGGGTCCATGCAATGAAG+TGG 0.619302 4_4:+43258390 Msa0682160:CDS
TAAGGTTCTTATATATGCAG+GGG 0.630544 4_4:+43258093 Msa0682160:CDS
TGAACCCTAGATGCAAGAGC+AGG 0.635146 4_4:-43256983 None:intergenic
CTTCATGTATAATGTTTGGA+GGG 0.639974 4_4:+43258813 Msa0682160:three_prime_UTR
AACGAACAATAACAAAAGAG+AGG 0.642414 4_4:-43255068 None:intergenic
TTCCTCGTTCCAAATCCGCA+AGG 0.666060 4_4:+43255356 Msa0682160:CDS
CTAACCTTTAGAAAAGAGAG+AGG 0.669936 4_4:-43258497 None:intergenic
GAAGCGAATAGTATCCGGCA+TGG 0.671731 4_4:-43255336 None:intergenic
GAATGATTATGGTTGGGACA+AGG 0.673972 4_4:+43256268 Msa0682160:CDS
ATGGGAACTTGAATAGGACA+AGG 0.685574 4_4:+43258693 Msa0682160:CDS
TGATGTGTAACAGGTGAATG+GGG 0.699009 4_4:+43258607 Msa0682160:intron
GTTGTGATATGGTTGACTGG+AGG 0.703410 4_4:+43256167 Msa0682160:CDS
CTTGCATCTAGGGTTCACCA+TGG 0.722490 4_4:+43256989 Msa0682160:CDS
AGTCAACCATATCACAACTG+GGG 0.752820 4_4:-43256162 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! TCTAACTATTTAATTAAATA+TGG - chr4_4:43255689-43255708 None:intergenic 10.0%
!!! TTTTTATTTATAATAATCAC+AGG + chr4_4:43256883-43256902 Msa0682160:intron 10.0%
!!! AAATACAAGATTTTTAATTG+CGG - chr4_4:43255507-43255526 None:intergenic 15.0%
!! ATTCAAAATATATTATTGTG+TGG + chr4_4:43255567-43255586 Msa0682160:intron 15.0%
!!! TTAGCTATAATTTTATTAGT+CGG + chr4_4:43255821-43255840 Msa0682160:intron 15.0%
!!! TAAAATTTGACTTAATTTTG+TGG + chr4_4:43256051-43256070 Msa0682160:intron 15.0%
!!! TCTTTTTTTTATTGAATTTC+AGG + chr4_4:43256096-43256115 Msa0682160:intron 15.0%
!!! CTAAATATCTCTAAAATTTA+CGG - chr4_4:43256491-43256510 None:intergenic 15.0%
!! TGTCAATAATAAAACATATT+TGG - chr4_4:43256618-43256637 None:intergenic 15.0%
!! AATGCTTAAAGATATTAATT+GGG - chr4_4:43256813-43256832 None:intergenic 15.0%
!! TAATGCTTAAAGATATTAAT+TGG - chr4_4:43256814-43256833 None:intergenic 15.0%
!!! CAAACTTATTTTATTTGAAT+TGG + chr4_4:43257676-43257695 Msa0682160:intron 15.0%
!!! AATTTGATTTTATTTGCAAT+TGG + chr4_4:43258116-43258135 Msa0682160:CDS 15.0%
!! AACAATTTGAATTTAAAAGA+GGG - chr4_4:43258154-43258173 None:intergenic 15.0%
!! AAACAATTTGAATTTAAAAG+AGG - chr4_4:43258155-43258174 None:intergenic 15.0%
!!! AATTTATTTATTTTTCCCAA+TGG - chr4_4:43258180-43258199 None:intergenic 15.0%
!! ATTGGGAAAAATAAATAAAT+TGG + chr4_4:43258179-43258198 Msa0682160:intron 15.0%
!! ATACAAATTTAATTACCTTG+CGG - chr4_4:43255374-43255393 None:intergenic 20.0%
!! AATCATTCAATCAAAATTGA+AGG - chr4_4:43255550-43255569 None:intergenic 20.0%
!! GTATATTATTCAGAACAAAT+CGG - chr4_4:43255801-43255820 None:intergenic 20.0%
!!! ACTATTAATATTTGGAATGT+TGG - chr4_4:43256013-43256032 None:intergenic 20.0%
!! AACATTCCAAATATTAATAG+TGG + chr4_4:43256012-43256031 Msa0682160:intron 20.0%
!! TTCAAACCACTATTAATATT+TGG - chr4_4:43256021-43256040 None:intergenic 20.0%
!! TGAATCAAGAAAAAATTCTA+AGG + chr4_4:43256136-43256155 Msa0682160:CDS 20.0%
!!! AAGTTATTGGCAATTTTAAA+AGG - chr4_4:43256236-43256255 None:intergenic 20.0%
!!! TTTACAATGGTTTTAAATTG+CGG + chr4_4:43256345-43256364 Msa0682160:intron 20.0%
!! ATTATTGACATTATGACTAA+AGG + chr4_4:43256628-43256647 Msa0682160:intron 20.0%
!! AAAGATATTAATTGGGATTA+TGG - chr4_4:43256806-43256825 None:intergenic 20.0%
!!! CATATAGTATACACATTTTT+TGG - chr4_4:43256848-43256867 None:intergenic 20.0%
!! TGATAACAAAATGATTACAA+AGG + chr4_4:43257408-43257427 Msa0682160:CDS 20.0%
!! TTGACAATACTAGTAAAAAT+GGG - chr4_4:43257524-43257543 None:intergenic 20.0%
!! TTTGACAATACTAGTAAAAA+TGG - chr4_4:43257525-43257544 None:intergenic 20.0%
!! TTGTCTATGATAATATAGTA+CGG + chr4_4:43257611-43257630 Msa0682160:intron 20.0%
!!! AATTCAAATTGTTTTACCAT+TGG + chr4_4:43258161-43258180 Msa0682160:intron 20.0%
!!! ATTCAAATTGTTTTACCATT+GGG + chr4_4:43258162-43258181 Msa0682160:intron 20.0%
!!! TTTGCTATTTTATTCTCATT+TGG + chr4_4:43258354-43258373 Msa0682160:intron 20.0%
!!! TTGCTATTTTATTCTCATTT+GGG + chr4_4:43258355-43258374 Msa0682160:intron 20.0%
! ATTATATTAACTGGATCCTT+TGG - chr4_4:43255211-43255230 None:intergenic 25.0%
! GATCCAGTTAATATAATTCA+TGG + chr4_4:43255214-43255233 Msa0682160:CDS 25.0%
! TCACCATGAATTATATTAAC+TGG - chr4_4:43255220-43255239 None:intergenic 25.0%
! TCAATCAAAATTGAAGGAAT+CGG - chr4_4:43255544-43255563 None:intergenic 25.0%
! TGAATTTGTGAAATGAAGTT+AGG + chr4_4:43255616-43255635 Msa0682160:intron 25.0%
! AGTTAGAATAAAATGTCATG+TGG + chr4_4:43255702-43255721 Msa0682160:intron 25.0%
! ATGTGACTATATAGATCTTA+AGG + chr4_4:43255858-43255877 Msa0682160:intron 25.0%
! TTACTTGTAGTACAATGTTA+TGG - chr4_4:43255886-43255905 None:intergenic 25.0%
!!! ATATCTCTAAAATTTACGGT+CGG - chr4_4:43256487-43256506 None:intergenic 25.0%
!!! AGGTGTTTTTTTCATTTTGT+AGG + chr4_4:43256648-43256667 Msa0682160:intron 25.0%
!!! TACACATTTTTTGGTATATG+TGG - chr4_4:43256839-43256858 None:intergenic 25.0%
! AGTCATTCTTAACAAACTTA+GGG - chr4_4:43256925-43256944 None:intergenic 25.0%
! AAGTCATTCTTAACAAACTT+AGG - chr4_4:43256926-43256945 None:intergenic 25.0%
! AAGAATGACTTCTATATTAC+TGG + chr4_4:43256935-43256954 Msa0682160:CDS 25.0%
! ATGGAAATAAAGGCAAAAAA+GGG + chr4_4:43257008-43257027 Msa0682160:CDS 25.0%
! CCTATCATAATCATAAACAA+TGG - chr4_4:43257261-43257280 None:intergenic 25.0%
! CCATTGTTTATGATTATGAT+AGG + chr4_4:43257258-43257277 Msa0682160:intron 25.0%
! TTTGATTCCAATACAATTAC+AGG + chr4_4:43257318-43257337 Msa0682160:intron 25.0%
! TTGATTCCAATACAATTACA+GGG + chr4_4:43257319-43257338 Msa0682160:intron 25.0%
!! AATAATATTTTGTCCATTGC+CGG + chr4_4:43257758-43257777 Msa0682160:CDS 25.0%
! ATTAAGGTTCTTATATATGC+AGG + chr4_4:43258091-43258110 Msa0682160:CDS 25.0%
! TTAAGGTTCTTATATATGCA+GGG + chr4_4:43258092-43258111 Msa0682160:CDS 25.0%
! ACAATGCATTGTTATATACA+GGG - chr4_4:43258276-43258295 None:intergenic 25.0%
! AACAATGCATTGTTATATAC+AGG - chr4_4:43258277-43258296 None:intergenic 25.0%
!! ATCCAAAACAGTTCAATTTT+TGG + chr4_4:43258436-43258455 Msa0682160:CDS 25.0%
!! AACCAAAAATTGAACTGTTT+TGG - chr4_4:43258441-43258460 None:intergenic 25.0%
!!! ACAGTTCAATTTTTGGTTAA+TGG + chr4_4:43258443-43258462 Msa0682160:CDS 25.0%
! GCAGGATTGTTAAATATTTA+TGG + chr4_4:43258473-43258492 Msa0682160:CDS 25.0%
! ATGACTTCATGTATAATGTT+TGG + chr4_4:43258809-43258828 Msa0682160:three_prime_UTR 25.0%
AACGAACAATAACAAAAGAG+AGG - chr4_4:43255071-43255090 None:intergenic 30.0%
! AAGCTTTAACTTATTGCCAA+AGG + chr4_4:43255192-43255211 Msa0682160:CDS 30.0%
ATTTAATTACCTTGCGGATT+TGG - chr4_4:43255368-43255387 None:intergenic 30.0%
ATAGTACAATTGCACAATTG+CGG + chr4_4:43255437-43255456 Msa0682160:intron 30.0%
GTGATACATTCACAATATTG+CGG - chr4_4:43255474-43255493 None:intergenic 30.0%
ATATTATTGTGTGGAGTCAA+AGG + chr4_4:43255576-43255595 Msa0682160:intron 30.0%
!!! CTAGCTTTGTTTTATGAGAA+TGG + chr4_4:43256209-43256228 Msa0682160:CDS 30.0%
! TCTCTTTTGTGGAATGATTA+TGG + chr4_4:43256257-43256276 Msa0682160:CDS 30.0%
! TTTTGTGGAATGATTATGGT+TGG + chr4_4:43256261-43256280 Msa0682160:CDS 30.0%
TTTGTGGAATGATTATGGTT+GGG + chr4_4:43256262-43256281 Msa0682160:CDS 30.0%
TGTGTCATATGAGTTTACAA+TGG + chr4_4:43256332-43256351 Msa0682160:intron 30.0%
TCATTGTCAATCAATGTCTT+TGG - chr4_4:43256579-43256598 None:intergenic 30.0%
! TTTTTGTTGACCAACCAATT+GGG + chr4_4:43256683-43256702 Msa0682160:CDS 30.0%
!!! CTTTTTTGCCTTTATTTCCA+TGG - chr4_4:43257009-43257028 None:intergenic 30.0%
CATGGAAATAAAGGCAAAAA+AGG + chr4_4:43257007-43257026 Msa0682160:CDS 30.0%
ATGTTATGTATCGTCAATCA+CGG - chr4_4:43257146-43257165 None:intergenic 30.0%
AACATTTGATGTCTCTATTG+CGG + chr4_4:43257161-43257180 Msa0682160:intron 30.0%
GACTTTATCAATACTGCATT+TGG + chr4_4:43257183-43257202 Msa0682160:intron 30.0%
TTGTTCTGCAATACACTATT+TGG + chr4_4:43257579-43257598 Msa0682160:intron 30.0%
TCACAAATCGCAAAAAATAG+CGG - chr4_4:43257652-43257671 None:intergenic 30.0%
TATCTGTTCACATTGTTGTA+AGG - chr4_4:43257735-43257754 None:intergenic 30.0%
AGTACTTACATTAACGTTAC+CGG - chr4_4:43257780-43257799 None:intergenic 30.0%
GACATAAGAAAGAAATGTCA+AGG + chr4_4:43257890-43257909 Msa0682160:CDS 30.0%
TTCTAAACAAGAAGACAGTT+AGG + chr4_4:43257948-43257967 Msa0682160:CDS 30.0%
TCTAAACAAGAAGACAGTTA+GGG + chr4_4:43257949-43257968 Msa0682160:CDS 30.0%
ATAATGCAATGTTGCAAGAT+TGG + chr4_4:43258023-43258042 Msa0682160:CDS 30.0%
TAAGGTTCTTATATATGCAG+GGG + chr4_4:43258093-43258112 Msa0682160:CDS 30.0%
!! GATTTTATTTGCAATTGGCT+TGG + chr4_4:43258121-43258140 Msa0682160:CDS 30.0%
GTGGCTATAATTCATCTTTA+AGG + chr4_4:43258215-43258234 Msa0682160:intron 30.0%
CAATGCATTGTTATATACAG+GGG - chr4_4:43258275-43258294 None:intergenic 30.0%
! TATTTTATTCTCATTTGGGC+AGG + chr4_4:43258359-43258378 Msa0682160:intron 30.0%
! ATTTTATTCTCATTTGGGCA+GGG + chr4_4:43258360-43258379 Msa0682160:intron 30.0%
TTGGTTAATGGTAAAAAAGC+AGG + chr4_4:43258455-43258474 Msa0682160:CDS 30.0%
! GACACTGTTGATTAAACTTT+AGG + chr4_4:43258569-43258588 Msa0682160:intron 30.0%
TGATTTATGTGATGTGTAAC+AGG + chr4_4:43258598-43258617 Msa0682160:intron 30.0%
ACTTCATGTATAATGTTTGG+AGG + chr4_4:43258812-43258831 Msa0682160:three_prime_UTR 30.0%
CTTCATGTATAATGTTTGGA+GGG + chr4_4:43258813-43258832 Msa0682160:three_prime_UTR 30.0%
!!! GGTGAAAATTTTGGTGTTGA+TGG - chr4_4:43255145-43255164 None:intergenic 35.0%
!!! CGTTGGTTTGGTGAAAATTT+TGG - chr4_4:43255154-43255173 None:intergenic 35.0%
! TTATAAGCTTTTCAGCTCGT+TGG - chr4_4:43255171-43255190 None:intergenic 35.0%
! TCTATTGATCATTTTGTCCC+TGG + chr4_4:43255244-43255263 Msa0682160:CDS 35.0%
ATCTTCAATAGAAGGACCAT+CGG - chr4_4:43255308-43255327 None:intergenic 35.0%
GACTATAGTGACGATGTAAA+CGG + chr4_4:43255638-43255657 Msa0682160:intron 35.0%
! TCGAACATAAGCATTGCTTT+CGG - chr4_4:43255751-43255770 None:intergenic 35.0%
CCACAAAAGAGACAAGTTAT+TGG - chr4_4:43256249-43256268 None:intergenic 35.0%
!! CCAATAACTTGTCTCTTTTG+TGG + chr4_4:43256246-43256265 Msa0682160:CDS 35.0%
CAACATCACAACTGAAATTG+TGG - chr4_4:43256440-43256459 None:intergenic 35.0%
! CTTTTTGTTGACCAACCAAT+TGG + chr4_4:43256682-43256701 Msa0682160:CDS 35.0%
GATATTCCACATGATGAAAC+TGG + chr4_4:43256736-43256755 Msa0682160:CDS 35.0%
ATTACTGGAGAATCATATGC+TGG + chr4_4:43256950-43256969 Msa0682160:CDS 35.0%
AGGGATTACTATAAACCTCA+AGG + chr4_4:43257027-43257046 Msa0682160:CDS 35.0%
TAGGACATTGTATAACCTTG+AGG - chr4_4:43257045-43257064 None:intergenic 35.0%
GCAAAACCCTGTAATTGTAT+TGG - chr4_4:43257328-43257347 None:intergenic 35.0%
!! CAATTACAGGGTTTTGCTAT+TGG + chr4_4:43257331-43257350 Msa0682160:intron 35.0%
!!! AGGGTTTTGCTATTGGTAAT+GGG + chr4_4:43257338-43257357 Msa0682160:intron 35.0%
! TCTTGTACCGCAAGTTTTTA+TGG - chr4_4:43257479-43257498 None:intergenic 35.0%
! GGTGTGATAAGAGTGTATAA+TGG - chr4_4:43257503-43257522 None:intergenic 35.0%
ACTAGTATTGTCAAATAGCG+CGG + chr4_4:43257530-43257549 Msa0682160:intron 35.0%
TTTGAATTGGCAGAGAGTAA+AGG + chr4_4:43257689-43257708 Msa0682160:intron 35.0%
! TTGGATGAGAAACTTTGAAG+TGG + chr4_4:43258042-43258061 Msa0682160:CDS 35.0%
ACTTCTTGAGGATGGAATTA+AGG + chr4_4:43258075-43258094 Msa0682160:CDS 35.0%
AGGTTAATAAGTGAGATGTC+TGG + chr4_4:43258235-43258254 Msa0682160:intron 35.0%
GGTTAATAAGTGAGATGTCT+GGG + chr4_4:43258236-43258255 Msa0682160:intron 35.0%
GTTAATAAGTGAGATGTCTG+GGG + chr4_4:43258237-43258256 Msa0682160:intron 35.0%
TTCTTCGATCATAACTCAGT+TGG - chr4_4:43258304-43258323 None:intergenic 35.0%
! CTAACCTTTAGAAAAGAGAG+AGG - chr4_4:43258500-43258519 None:intergenic 35.0%
CATCCTCTAACATTGAGATA+AGG + chr4_4:43258766-43258785 Msa0682160:three_prime_UTR 35.0%
GATCCTTATCTCAATGTTAG+AGG - chr4_4:43258772-43258791 None:intergenic 35.0%
! TCTTCTCGACGATTTTTCCA+GGG - chr4_4:43255264-43255283 None:intergenic 40.0%
! TTCTTCTCGACGATTTTTCC+AGG - chr4_4:43255265-43255284 None:intergenic 40.0%
GGTCCTTCTATTGAAGATCT+CGG + chr4_4:43255310-43255329 Msa0682160:CDS 40.0%
TGACCGAGATCTTCAATAGA+AGG - chr4_4:43255316-43255335 None:intergenic 40.0%
AGTCAACCATATCACAACTG+GGG - chr4_4:43256165-43256184 None:intergenic 40.0%
CAGTCAACCATATCACAACT+GGG - chr4_4:43256166-43256185 None:intergenic 40.0%
GAATGATTATGGTTGGGACA+AGG + chr4_4:43256268-43256287 Msa0682160:CDS 40.0%
TAAAACCTGTCCCAATTGGT+TGG - chr4_4:43256696-43256715 None:intergenic 40.0%
TAGCTAAAACCTGTCCCAAT+TGG - chr4_4:43256700-43256719 None:intergenic 40.0%
CTAACACCAGTTTCATCATG+TGG - chr4_4:43256745-43256764 None:intergenic 40.0%
! TGATTTGTACGACTTCTTGC+TGG + chr4_4:43256768-43256787 Msa0682160:CDS 40.0%
AGGGTTCACCATGGAAATAA+AGG + chr4_4:43256998-43257017 Msa0682160:CDS 40.0%
!!! CAGGGTTTTGCTATTGGTAA+TGG + chr4_4:43257337-43257356 Msa0682160:intron 40.0%
! TTGGTACTGAATTGCTGGAT+TGG - chr4_4:43257368-43257387 None:intergenic 40.0%
! TATGCTTGGTACTGAATTGC+TGG - chr4_4:43257373-43257392 None:intergenic 40.0%
! ATGCAAACTGAGAGTATGCT+TGG - chr4_4:43257387-43257406 None:intergenic 40.0%
GAGGTTGCCATAAAAACTTG+CGG + chr4_4:43257469-43257488 Msa0682160:CDS 40.0%
TACATTAACGTTACCGGCAA+TGG - chr4_4:43257774-43257793 None:intergenic 40.0%
AAGACAGTTAGGGATGCTTT+AGG + chr4_4:43257959-43257978 Msa0682160:CDS 40.0%
! TTAGGGATGCTTTAGGTGTT+GGG + chr4_4:43257966-43257985 Msa0682160:CDS 40.0%
GGATATTCCTGAACTTCTTG+AGG + chr4_4:43258063-43258082 Msa0682160:CDS 40.0%
ATTCCTGAACTTCTTGAGGA+TGG + chr4_4:43258067-43258086 Msa0682160:CDS 40.0%
ATTCCATCCTCAAGAAGTTC+AGG - chr4_4:43258073-43258092 None:intergenic 40.0%
! AATTGGTTCAAGCAGTCTAG+TGG + chr4_4:43258196-43258215 Msa0682160:intron 40.0%
TTTGGGCAGGGAATTCAAAA+TGG + chr4_4:43258372-43258391 Msa0682160:intron 40.0%
TTGGGCAGGGAATTCAAAAT+GGG + chr4_4:43258373-43258392 Msa0682160:intron 40.0%
! TGGACCTCTCTCTTTTCTAA+AGG + chr4_4:43258493-43258512 Msa0682160:CDS 40.0%
TGTGATGTGTAACAGGTGAA+TGG + chr4_4:43258605-43258624 Msa0682160:intron 40.0%
GTGATGTGTAACAGGTGAAT+GGG + chr4_4:43258606-43258625 Msa0682160:intron 40.0%
TGATGTGTAACAGGTGAATG+GGG + chr4_4:43258607-43258626 Msa0682160:intron 40.0%
!! CAGCTTTTGGTTGATCCATA+GGG - chr4_4:43258646-43258665 None:intergenic 40.0%
!! AACATCTGAAGTGCAGCTTT+TGG - chr4_4:43258659-43258678 None:intergenic 40.0%
AAAAGCTGCACTTCAGATGT+TGG + chr4_4:43258658-43258677 Msa0682160:CDS 40.0%
ATGGGAACTTGAATAGGACA+AGG + chr4_4:43258693-43258712 Msa0682160:CDS 40.0%
!! AGCTTTTCAGCTCGTTGGTT+TGG - chr4_4:43255166-43255185 None:intergenic 45.0%
!! TCGTTTTTCAGCGATTCCGA+TGG + chr4_4:43255289-43255308 Msa0682160:CDS 45.0%
ACGAGGAAGCGAATAGTATC+CGG - chr4_4:43255344-43255363 None:intergenic 45.0%
GCTTATGTTCGATGAGATCC+AGG + chr4_4:43255759-43255778 Msa0682160:intron 45.0%
TCGATGAGATCCAGGTCAAA+AGG + chr4_4:43255767-43255786 Msa0682160:intron 45.0%
! GGTACAACAACCTTTTGACC+TGG - chr4_4:43255780-43255799 None:intergenic 45.0%
CCAGTCAACCATATCACAAC+TGG - chr4_4:43256167-43256186 None:intergenic 45.0%
CCAGTTGTGATATGGTTGAC+TGG + chr4_4:43256164-43256183 Msa0682160:CDS 45.0%
GTTGTGATATGGTTGACTGG+AGG + chr4_4:43256167-43256186 Msa0682160:CDS 45.0%
ATGTTCCTGCTCTTGCATCT+AGG + chr4_4:43256978-43256997 Msa0682160:CDS 45.0%
TGTTCCTGCTCTTGCATCTA+GGG + chr4_4:43256979-43256998 Msa0682160:CDS 45.0%
CATTTGGACCTCGACAAATG+AGG + chr4_4:43257199-43257218 Msa0682160:intron 45.0%
GTTACGATCCTCATTTGTCG+AGG - chr4_4:43257210-43257229 None:intergenic 45.0%
TTATGGCAACCTCGCAATCT+GGG - chr4_4:43257462-43257481 None:intergenic 45.0%
TTTATGGCAACCTCGCAATC+TGG - chr4_4:43257463-43257482 None:intergenic 45.0%
GAATTGGCAGAGAGTAAAGG+TGG + chr4_4:43257692-43257711 Msa0682160:intron 45.0%
! GTTAGGGATGCTTTAGGTGT+TGG + chr4_4:43257965-43257984 Msa0682160:CDS 45.0%
! TAGGGATGCTTTAGGTGTTG+GGG + chr4_4:43257967-43257986 Msa0682160:CDS 45.0%
AATGGGTCCATGCAATGAAG+TGG + chr4_4:43258390-43258409 Msa0682160:CDS 45.0%
!! GCAGCTTTTGGTTGATCCAT+AGG - chr4_4:43258647-43258666 None:intergenic 45.0%
CACTTCAGATGTTGGCAAAC+TGG + chr4_4:43258666-43258685 Msa0682160:CDS 45.0%
ATGTTGGCAAACTGGATGCA+TGG + chr4_4:43258674-43258693 Msa0682160:CDS 45.0%
TGTTGGCAAACTGGATGCAT+GGG + chr4_4:43258675-43258694 Msa0682160:CDS 45.0%
GGATGCATGGGAACTTGAAT+AGG + chr4_4:43258687-43258706 Msa0682160:CDS 45.0%
!! ATTCATAATATAAAATTAAT+AGG - chr4_4:43257064-43257083 None:intergenic 5.0%
!!! AAGATAAAATAAATTTTTTT+CGG - chr4_4:43257815-43257834 None:intergenic 5.0%
GATGCGAGAAGTTGCGTATG+AGG - chr4_4:43255104-43255123 None:intergenic 50.0%
GAAGCGAATAGTATCCGGCA+TGG - chr4_4:43255339-43255358 None:intergenic 50.0%
TTCCTCGTTCCAAATCCGCA+AGG + chr4_4:43255356-43255375 Msa0682160:CDS 50.0%
! TACCTTGCGGATTTGGAACG+AGG - chr4_4:43255361-43255380 None:intergenic 50.0%
AGGATGCCCCAGTTGTGATA+TGG + chr4_4:43256156-43256175 Msa0682160:CDS 50.0%
ATATGGTTGACTGGAGGTCC+AGG + chr4_4:43256173-43256192 Msa0682160:CDS 50.0%
GTTGACCAACCAATTGGGAC+AGG + chr4_4:43256688-43256707 Msa0682160:CDS 50.0%
TGAACCCTAGATGCAAGAGC+AGG - chr4_4:43256986-43257005 None:intergenic 50.0%
! CTTGCATCTAGGGTTCACCA+TGG + chr4_4:43256989-43257008 Msa0682160:CDS 50.0%
!! CAAGTTGATCCCAGATTGCG+AGG + chr4_4:43257450-43257469 Msa0682160:CDS 50.0%
GTCCATGCAATGAAGTGGTC+AGG + chr4_4:43258395-43258414 Msa0682160:CDS 50.0%
GACCTGACCACTTCATTGCA+TGG - chr4_4:43258400-43258419 None:intergenic 50.0%
GAAGATCTCGGTCACCATGC+CGG + chr4_4:43255322-43255341 Msa0682160:CDS 55.0%
GCTAGTTCACTGCTGCAACC+TGG - chr4_4:43256194-43256213 None:intergenic 55.0%
GTGTAACAGGTGAATGGGGC+AGG + chr4_4:43258611-43258630 Msa0682160:intron 55.0%
GGTGAATGGGGCAGGACATA+TGG + chr4_4:43258619-43258638 Msa0682160:intron 55.0%
GGCAGGACATATGGTCCCTA+TGG + chr4_4:43258628-43258647 Msa0682160:CDS 55.0%
Chromosome Type Strat End Strand Name
chr4_4 gene 43255052 43258837 43255052 ID=Msa0682160;Name=Msa0682160
chr4_4 mRNA 43255052 43258837 43255052 ID=Msa0682160-mRNA-1;Parent=Msa0682160;Name=Msa0682160-mRNA-1;_AED=0.30;_eAED=0.30;_QI=0|1|0.77|1|1|1|9|116|498
chr4_4 exon 43255052 43255377 43255052 ID=Msa0682160-mRNA-1:exon:10491;Parent=Msa0682160-mRNA-1
chr4_4 exon 43256118 43256289 43256118 ID=Msa0682160-mRNA-1:exon:10492;Parent=Msa0682160-mRNA-1
chr4_4 exon 43256670 43256789 43256670 ID=Msa0682160-mRNA-1:exon:10493;Parent=Msa0682160-mRNA-1
chr4_4 exon 43256905 43257048 43256905 ID=Msa0682160-mRNA-1:exon:10494;Parent=Msa0682160-mRNA-1
chr4_4 exon 43257340 43257490 43257340 ID=Msa0682160-mRNA-1:exon:10495;Parent=Msa0682160-mRNA-1
chr4_4 exon 43257702 43257790 43257702 ID=Msa0682160-mRNA-1:exon:10496;Parent=Msa0682160-mRNA-1
chr4_4 exon 43257884 43258142 43257884 ID=Msa0682160-mRNA-1:exon:10497;Parent=Msa0682160-mRNA-1
chr4_4 exon 43258381 43258514 43258381 ID=Msa0682160-mRNA-1:exon:10498;Parent=Msa0682160-mRNA-1
chr4_4 exon 43258620 43258837 43258620 ID=Msa0682160-mRNA-1:exon:10499;Parent=Msa0682160-mRNA-1
chr4_4 CDS 43255052 43255377 43255052 ID=Msa0682160-mRNA-1:cds;Parent=Msa0682160-mRNA-1
chr4_4 CDS 43256118 43256289 43256118 ID=Msa0682160-mRNA-1:cds;Parent=Msa0682160-mRNA-1
chr4_4 CDS 43256670 43256789 43256670 ID=Msa0682160-mRNA-1:cds;Parent=Msa0682160-mRNA-1
chr4_4 CDS 43256905 43257048 43256905 ID=Msa0682160-mRNA-1:cds;Parent=Msa0682160-mRNA-1
chr4_4 CDS 43257340 43257490 43257340 ID=Msa0682160-mRNA-1:cds;Parent=Msa0682160-mRNA-1
chr4_4 CDS 43257702 43257790 43257702 ID=Msa0682160-mRNA-1:cds;Parent=Msa0682160-mRNA-1
chr4_4 CDS 43257884 43258142 43257884 ID=Msa0682160-mRNA-1:cds;Parent=Msa0682160-mRNA-1
chr4_4 CDS 43258381 43258514 43258381 ID=Msa0682160-mRNA-1:cds;Parent=Msa0682160-mRNA-1
chr4_4 CDS 43258620 43258721 43258620 ID=Msa0682160-mRNA-1:cds;Parent=Msa0682160-mRNA-1
chr4_4 three_prime_UTR 43258722 43258837 43258722 ID=Msa0682160-mRNA-1:three_prime_utr;Parent=Msa0682160-mRNA-1
Gene Sequence

>Msa0682160

ATGGGATTCTCTAAAACCTCTCTTTTGTTATTGTTCGTTTTGTTTTCTTCCTCATACGCAACTTCTCGCATCACACATCATAGAAGTTTTCCATCAACACCAAAATTTTCACCAAACCAACGAGCTGAAAAGCTTATAAAAAGCTTTAACTTATTGCCAAAGGATCCAGTTAATATAATTCATGGTGATCACTCTATTGATCATTTTGTCCCTGGAAAAATCGTCGAGAAGAAGTTCTCGTTTTTCAGCGATTCCGATGGTCCTTCTATTGAAGATCTCGGTCACCATGCCGGATACTATTCGCTTCCTCGTTCCAAATCCGCAAGGATGTTCTATTTTTTCTTTGAATCAAGAAAAAATTCTAAGGATGCCCCAGTTGTGATATGGTTGACTGGAGGTCCAGGTTGCAGCAGTGAACTAGCTTTGTTTTATGAGAATGGTCCTTTTAAAATTGCCAATAACTTGTCTCTTTTGTGGAATGATTATGGTTGGGACAAGGCATCAAATATTCTTTTTGTTGACCAACCAATTGGGACAGGTTTTAGCTATACGTCTAATGAAGATGATATTCCACATGATGAAACTGGTGTTAGCAATGATTTGTACGACTTCTTGCTGGAGTTTTTCAAGCAGCACCCTAAGTTTGTTAAGAATGACTTCTATATTACTGGAGAATCATATGCTGGACACTATGTTCCTGCTCTTGCATCTAGGGTTCACCATGGAAATAAAGGCAAAAAAGGGATTACTATAAACCTCAAGGGTTTTGCTATTGGTAATGGGTTGACCAATCCAGCAATTCAGTACCAAGCATACTCTCAGTTTGCATTTGATAACAAAATGATTACAAAGGAAGATCAAACTGATATCAACAAGTTGATCCCAGATTGCGAGGTTGCCATAAAAACTTGCGAGAGTAAAGGTGGAGAAAGTTGCTATTCAGCCTTACAACAATGTGAACAGATAATCAATAATATTTTGTCCATTGCCGGTAACGTTAATTACTATGACATAAGAAAGAAATGTCAAGGATCATTGTGTTATGACTTCTCAAATTTAGAGAAACTTCTAAACAAGAAGACAGTTAGGGATGCTTTAGGTGTTGGGGACATAGACTTTGTTTCATGCAGCAAAGAAGTGTATAATGCAATGTTGCAAGATTGGATGAGAAACTTTGAAGTGGATATTCCTGAACTTCTTGAGGATGGAATTAAGGTTCTTATATATGCAGGGGAATTTGATTTTATTTGCAATTGGCTTGGGAATTCAAAATGGGTCCATGCAATGAAGTGGTCAGGTCAAAAGAAATTTGCAGCATCCAAAACAGTTCAATTTTTGGTTAATGGTAAAAAAGCAGGATTGTTAAATATTTATGGACCTCTCTCTTTTCTAAAGGTGAATGGGGCAGGACATATGGTCCCTATGGATCAACCAAAAGCTGCACTTCAGATGTTGGCAAACTGGATGCATGGGAACTTGAATAGGACAAGGATTTGA
Protein sequence

>Msa0682160

MGFSKTSLLLLFVLFSSSYATSRITHHRSFPSTPKFSPNQRAEKLIKSFNLLPKDPVNIIHGDHSIDHFVPGKIVEKKFSFFSDSDGPSIEDLGHHAGYYSLPRSKSARMFYFFFESRKNSKDAPVVIWLTGGPGCSSELALFYENGPFKIANNLSLLWNDYGWDKASNILFVDQPIGTGFSYTSNEDDIPHDETGVSNDLYDFLLEFFKQHPKFVKNDFYITGESYAGHYVPALASRVHHGNKGKKGITINLKGFAIGNGLTNPAIQYQAYSQFAFDNKMITKEDQTDINKLIPDCEVAIKTCESKGGESCYSALQQCEQIINNILSIAGNVNYYDIRKKCQGSLCYDFSNLEKLLNKKTVRDALGVGDIDFVSCSKEVYNAMLQDWMRNFEVDIPELLEDGIKVLIYAGEFDFICNWLGNSKWVHAMKWSGQKKFAASKTVQFLVNGKKAGLLNIYGPLSFLKVNGAGHMVPMDQPKAALQMLANWMHGNLNRTRI*