AlfalfaGEDB | Msa0375800

M. sativa cultivar zhongmu-4 / Msa0375800

KEGG: -
GO: -
PFAM: PF08534.13

Nr:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa0375800	XP_003602160.1	94.142	239	12	1	1	237	1	239	3.91e-163	461

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Swissprot:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
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Trembl:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa0375800	G7J667	94.142	239	12	1	1	237	1	239	1.87e-163	461

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TFs/TRs:

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Gene ID	Type	Classification
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Protein Kinases:

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Gene ID	Type	Classification
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Network:

Co-expression Network

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Gene1	Gene2	correlation coefficient	p_value	FDR
Msa0010550	Msa0375800	0.819861	9.298877e-53	-8.615850e-47
Msa0051010	Msa0375800	0.843311	1.515305e-58	-8.615850e-47
Msa0101280	Msa0375800	0.838033	3.655907e-57	-8.615850e-47
Msa0177570	Msa0375800	0.820754	5.802516e-53	-8.615850e-47
Msa0373640	Msa0375800	0.837191	6.013437e-57	-8.615850e-47
Msa0375800	Msa0416530	0.804208	2.412658e-49	-8.615850e-47
Msa0375800	Msa0633310	0.824236	9.002733e-54	-8.615850e-47
Msa0375800	Msa0937270	0.825006	5.931790e-54	-8.615850e-47
Msa0375800	Msa1028780	0.818632	1.771195e-52	-8.615850e-47
Msa0375800	Msa1030380	-0.812341	4.452330e-51	-8.615850e-47

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PPI

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Gene1	Gene2	Type
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Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa0375800	MtrunA17_Chr3g0126171	94.142	239	12	1	1	237	1	239	3.60e-167	461

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Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa0375800	AT1G21350.5	81.818	187	33	1	51	237	66	251	3.71e-112	323
Msa0375800	AT1G21350.3	81.818	187	33	1	51	237	53	238	5.16e-112	322
Msa0375800	AT1G21350.2	68.984	187	30	2	51	237	53	211	3.88e-86	255
Msa0375800	AT1G21350.4	86.957	138	17	1	100	237	1	137	1.01e-85	251
Msa0375800	AT1G21350.1	86.957	138	17	1	100	237	1	137	1.01e-85	251

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Find 81 sgRNAs with CRISPR-Local

Find 238 sgRNAs with CRISPR-GE

CRISPR-Local

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CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
AAAATTGAGGTTGTGAATAT+TGG	0.261480	3_1:+75630074	Msa0375800:five_prime_UTR
AACATACAAGAAACAAAGAA+TGG	0.478756	3_1:-75633950	None:intergenic
AACGAAGAAGATGATGATGC+TGG	0.522229	3_1:+75630051	Msa0375800:five_prime_UTR
AAGACAGCAACCTGAAATTG+AGG	0.522154	3_1:-75630333	None:intergenic
AATAATAACAGGATGGTCGA+AGG	0.587773	3_1:+75633277	Msa0375800:intron
AATAATAACATACCAGTAAC+TGG	0.338409	3_1:+75633348	Msa0375800:CDS
AATTAAATGTAAAAGTACCT+AGG	0.590010	3_1:-75633549	None:intergenic
ACAGATCAAAACTTCTTTCA+TGG	0.346318	3_1:-75633675	None:intergenic
ACAGGATGGACCAGAATTTA+TGG	0.289573	3_1:+75631969	Msa0375800:intron
ACATACAAGAAACAAAGAAT+GGG	0.480846	3_1:-75633949	None:intergenic

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CRISPR-GE

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sgRNA_sequence	Strand	Position	Region	GC_content
TTACTAGGACCTATTTGAAT+TGG	-	chr3_1:75631083-75631102	None:intergenic	30.0%
CATATGAACTCATTCTTACT+AGG	-	chr3_1:75631098-75631117	None:intergenic	30.0%
TTCTTCAGGTGTATAACAAA+CGG	-	chr3_1:75631671-75631690	None:intergenic	30.0%
GCTTACTGAATTCTATATGA+AGG	+	chr3_1:75631703-75631722	Msa0375800:CDS	30.0%
AATGAATTTAGTATCACCTG+GGG	-	chr3_1:75631890-75631909	None:intergenic	30.0%
GAAATGAATTTAGTATCACC+TGG	-	chr3_1:75631892-75631911	None:intergenic	30.0%
GTGATACTAAATTCATTTCC+TGG	+	chr3_1:75631893-75631912	Msa0375800:intron	30.0%
TTTCAATGTAGCTATATTCC+AGG	-	chr3_1:75631914-75631933	None:intergenic	30.0%
TTATGTATTCACAGTTCACA+TGG	+	chr3_1:75632069-75632088	Msa0375800:intron	30.0%
TACTCTGTTTAGCTATAATG+TGG	+	chr3_1:75632248-75632267	Msa0375800:intron	30.0%

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Chromosome	Type	Strat	End	Strand	Name
chr3_1	gene	75630018	75634041	75630018	ID=Msa0375800;Name=Msa0375800
chr3_1	mRNA	75630018	75634041	75630018	ID=Msa0375800-mRNA-1;Parent=Msa0375800;Name=Msa0375800-mRNA-1;_AED=0.11;_eAED=0.11;_QI=99\|1\|1\|1\|1\|1\|8\|472\|242
chr3_1	exon	75630018	75630344	75630018	ID=Msa0375800-mRNA-1:exon:17760;Parent=Msa0375800-mRNA-1
chr3_1	exon	75630557	75630622	75630557	ID=Msa0375800-mRNA-1:exon:17761;Parent=Msa0375800-mRNA-1
chr3_1	exon	75631644	75631724	75631644	ID=Msa0375800-mRNA-1:exon:17762;Parent=Msa0375800-mRNA-1
chr3_1	exon	75631839	75631892	75631839	ID=Msa0375800-mRNA-1:exon:17763;Parent=Msa0375800-mRNA-1
chr3_1	exon	75631973	75632041	75631973	ID=Msa0375800-mRNA-1:exon:17764;Parent=Msa0375800-mRNA-1
chr3_1	exon	75633112	75633174	75633112	ID=Msa0375800-mRNA-1:exon:17765;Parent=Msa0375800-mRNA-1
chr3_1	exon	75633288	75633373	75633288	ID=Msa0375800-mRNA-1:exon:17766;Parent=Msa0375800-mRNA-1
chr3_1	exon	75633488	75634041	75633488	ID=Msa0375800-mRNA-1:exon:17767;Parent=Msa0375800-mRNA-1
chr3_1	five_prime_UTR	75630018	75630116	75630018	ID=Msa0375800-mRNA-1:five_prime_utr;Parent=Msa0375800-mRNA-1
chr3_1	CDS	75630117	75630344	75630117	ID=Msa0375800-mRNA-1:cds;Parent=Msa0375800-mRNA-1
chr3_1	CDS	75630557	75630622	75630557	ID=Msa0375800-mRNA-1:cds;Parent=Msa0375800-mRNA-1
chr3_1	CDS	75631644	75631724	75631644	ID=Msa0375800-mRNA-1:cds;Parent=Msa0375800-mRNA-1
chr3_1	CDS	75631839	75631892	75631839	ID=Msa0375800-mRNA-1:cds;Parent=Msa0375800-mRNA-1
chr3_1	CDS	75631973	75632041	75631973	ID=Msa0375800-mRNA-1:cds;Parent=Msa0375800-mRNA-1
chr3_1	CDS	75633112	75633174	75633112	ID=Msa0375800-mRNA-1:cds;Parent=Msa0375800-mRNA-1
chr3_1	CDS	75633288	75633373	75633288	ID=Msa0375800-mRNA-1:cds;Parent=Msa0375800-mRNA-1
chr3_1	CDS	75633488	75633569	75633488	ID=Msa0375800-mRNA-1:cds;Parent=Msa0375800-mRNA-1
chr3_1	three_prime_UTR	75633570	75634041	75633570	ID=Msa0375800-mRNA-1:three_prime_utr;Parent=Msa0375800-mRNA-1

Gene Sequence

>Msa0375800

ATGGCACTAAGCCTAAGAGTGACTTCTGTTTGGTCACCATTTGTTCAGTTAGGTTCTACTTCCTCTTCTGGATTCTCACTCTCTCAACCATGCTCTGTTTCCAAACCGGGTTTTCATCTTAAACTCAATACTACAAGAAAGAACTCATACCCAACAAGTCTTGTTATTAGAGCTGCTAGAATTGAGTCCAAAGGTGTCACCTTGGGTTTCAGAGCCCCTCAATTTCAGCTTTCAGAGCCTCTGACTGGAAAGGTTTGGACATTGGAAGATTTTGAGGCTCATCCAGCTCTATTGGTTATGTTTATATGCAACCACTGTCCGTTTGTTATACACCTGAAGAAAGTCATCGTAAAGCTTACTGAATTCTATATGAAGAAAGGACTTGCTGTCGTTGCTATATCTTCAAATTCCGTAGCCACACACCCCCAGGATGGACCAGAATTTATGGCAGAAGATGCTAAATTGTTTGGTTATCCTTTTCCATACCTATATGACGAGTCACAGGATGTTGCTCGAGATTTTGGAGCAGTTTGTACACCAGAATTTTACCTTTTTAAAAAGGATGGTCGAAGGCCGTTTGAGCTGGTTTACCACGGTCAGTTTGATGATTCAAGGCCAAGTAATAATAACATACCAGTAACTGGAAGAGACTTGAGCTTGGCAATAGATCGTGTTCTGAGCGGCCAGCCAGTACCATCAGAGCAAAAACCTAGGTACTTTTACATTTAA

Protein sequence

>Msa0375800

MALSLRVTSVWSPFVQLGSTSSSGFSLSQPCSVSKPGFHLKLNTTRKNSYPTSLVIRAARIESKGVTLGFRAPQFQLSEPLTGKVWTLEDFEAHPALLVMFICNHCPFVIHLKKVIVKLTEFYMKKGLAVVAISSNSVATHPQDGPEFMAEDAKLFGYPFPYLYDESQDVARDFGAVCTPEFYLFKKDGRRPFELVYHGQFDDSRPSNNNIPVTGRDLSLAIDRVLSGQPVPSEQKPRYFYI*