AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa0385250

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score

Find 51 sgRNAs with CRISPR-Local

Find 238 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
CTCTCGGCTGGTTTGGTAAA+TGG 0.101033 3_1:+88748391 None:intergenic
TAACTGTTGTCTTGCATTTC+AGG 0.149843 3_1:+88748153 None:intergenic
AATTGGAAGCAATGGATATT+TGG 0.171850 3_1:+88748118 None:intergenic
AATCCCTTCTGCTACTCATT+TGG 0.287686 3_1:-88749681 Msa0385250:CDS
CGGAGGTGGAGTCTCCTTAC+AGG 0.322639 3_1:-88750888 Msa0385250:CDS
ATTTCCTACTACTTGGTTGC+AGG 0.334169 3_1:+88748352 None:intergenic
TCTCGGCTGGTTTGGTAAAT+GGG 0.341488 3_1:+88748392 None:intergenic
TTGGTATCCTGGGGAGTTGC+TGG 0.358040 3_1:-88749573 Msa0385250:CDS
GAAGTCTAGACTCTGGAGTA+TGG 0.387783 3_1:-88748221 Msa0385250:three_prime_UTR
AAACCAGCCGAGAGGAAGTT+TGG 0.393758 3_1:-88748382 Msa0385250:CDS
CGCCGTTGCAAACTTCATAT+TGG 0.407692 3_1:-88750829 Msa0385250:CDS
AAACTTCCTCTCGGCTGGTT+TGG 0.411065 3_1:+88748384 None:intergenic
TGGAAGTGTGGTAGCCTTGT+TGG 0.420953 3_1:-88749553 Msa0385250:CDS
GGTCCGACGGCTCTCGTCGC+TGG 0.432897 3_1:-88750867 Msa0385250:CDS
GTGACATATTTCCTACTACT+TGG 0.443763 3_1:+88748345 None:intergenic
TCACCAAACTTCCTCTCGGC+TGG 0.473061 3_1:+88748379 None:intergenic
GAGAAATAAATTGGAAGCAA+TGG 0.474395 3_1:+88748110 None:intergenic
TTTGGTGCTGATAGTGAGGC+AGG 0.478716 3_1:-88749516 Msa0385250:CDS
CGAGAGCCGTCGGACCTGTA+AGG 0.483398 3_1:+88750874 None:intergenic
GTCTTGCAAGGAGGCATACA+TGG 0.484785 3_1:+88748279 None:intergenic
GGCTCTCGTCGCTGGTGTGG+TGG 0.488726 3_1:-88750859 Msa0385250:CDS
CCAACCTGCAACCAAGTAGT+AGG 0.528997 3_1:-88748356 Msa0385250:exon
GACAACAGTTAAACAAAGAC+AGG 0.531151 3_1:-88748141 Msa0385250:three_prime_UTR
AGATCTATCTATAAAAGAAG+TGG 0.532978 3_1:+88748190 None:intergenic
AAACGATGATCTATCGGAGG+TGG 0.537673 3_1:-88750902 Msa0385250:exon
CCTACTACTTGGTTGCAGGT+TGG 0.538227 3_1:+88748356 None:intergenic
CGCTCCAAATGAGTAGCAGA+AGG 0.551961 3_1:+88749677 None:intergenic
GACGGCTCTCGTCGCTGGTG+TGG 0.553674 3_1:-88750862 Msa0385250:CDS
GCTGTCATAGTGATCGGTTG+TGG 0.560018 3_1:-88749651 Msa0385250:CDS
GTCGAATGAAGTCTAGACTC+TGG 0.572906 3_1:-88748228 Msa0385250:three_prime_UTR
TGGTGCTGATAGTGAGGCAG+GGG 0.578503 3_1:-88749514 Msa0385250:intron
GAAGTTTGCAACGGCGACAG+TGG 0.580249 3_1:+88750836 None:intergenic
TTGGTCACCAAACTTCCTCT+CGG 0.580621 3_1:+88748375 None:intergenic
TCAATTTGGTGCTGATAGTG+AGG 0.584055 3_1:-88749520 Msa0385250:CDS
CACACTTCCAGCAACTCCCC+AGG 0.591341 3_1:+88749566 None:intergenic
GACCAATATGAAGTTTGCAA+CGG 0.601164 3_1:+88750827 None:intergenic
CTGGGGAGTTGCTGGAAGTG+TGG 0.604577 3_1:-88749565 Msa0385250:CDS
TTGGTGCTGATAGTGAGGCA+GGG 0.617513 3_1:-88749515 Msa0385250:CDS
ACACCAGCGACGAGAGCCGT+CGG 0.623986 3_1:+88750864 None:intergenic
GCGGCTGCTGTCATAGTGAT+CGG 0.626473 3_1:-88749657 Msa0385250:CDS
ACTAGCGAACATGTCTTGCA+AGG 0.638822 3_1:+88748267 None:intergenic
GTCATAGTGATCGGTTGTGG+AGG 0.641605 3_1:-88749648 Msa0385250:CDS
CTTCTGCTACTCATTTGGAG+CGG 0.646845 3_1:-88749676 Msa0385250:CDS
GAGTCTCCTTACAGGTCCGA+CGG 0.651201 3_1:-88750880 Msa0385250:CDS
GTTAAGCTGCTAGCTTACCG+TGG 0.653587 3_1:-88747845 Msa0385250:three_prime_UTR
GCTCCAAATGAGTAGCAGAA+GGG 0.669509 3_1:+88749678 None:intergenic
AGCGAACATGTCTTGCAAGG+AGG 0.693031 3_1:+88748270 None:intergenic
TTGATGAAAATCTACCAACA+AGG 0.707898 3_1:+88749539 None:intergenic
GTAGCAGAAGGGATTAACCA+AGG 0.724082 3_1:+88749689 None:intergenic
CATTTACCAAACCAGCCGAG+AGG 0.725933 3_1:-88748390 Msa0385250:CDS
TAGTAGGAAATATGTCACAG+TGG 0.750184 3_1:-88748340 Msa0385250:three_prime_UTR

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!!! ATAGTTTTAATATTTAGTTA+TGG - chr3_1:88748557-88748576 Msa0385250:intron 10.0%
!!! TTGTTTTCATAAATAATAAT+AGG - chr3_1:88750749-88750768 Msa0385250:intron 10.0%
!!! TCATCGTTTTTTATTTTATT+TGG + chr3_1:88747892-88747911 None:intergenic 15.0%
!!! TTTTTCAACAAATATAAACA+GGG + chr3_1:88748061-88748080 None:intergenic 15.0%
!! AATGAAATTGATTAAATTAC+TGG + chr3_1:88748128-88748147 None:intergenic 15.0%
!! AATTTAATCAATTTCATTTG+AGG - chr3_1:88748130-88748149 Msa0385250:three_prime_UTR 15.0%
!! AATCAAATGATCATAATTAA+TGG + chr3_1:88748225-88748244 None:intergenic 15.0%
!!! TTTTAATATTTAGTTATGGA+AGG - chr3_1:88748561-88748580 Msa0385250:intron 15.0%
!! ACATAATATTTAACTCTTTA+GGG + chr3_1:88750357-88750376 None:intergenic 15.0%
!!! AATTTTAAGGAGAAATAAAT+TGG + chr3_1:88750707-88750726 None:intergenic 15.0%
!! AATAAAAAACGATGATCTAT+CGG - chr3_1:88747897-88747916 Msa0385250:three_prime_UTR 20.0%
!!! CTTTTTCAACAAATATAAAC+AGG + chr3_1:88748062-88748081 None:intergenic 20.0%
!! TCATTGAAATTGTGTAATTT+GGG - chr3_1:88748179-88748198 Msa0385250:three_prime_UTR 20.0%
!!! TAGTAGTAACATGTATTTTA+TGG - chr3_1:88748404-88748423 Msa0385250:intron 20.0%
!!! AGTAGTAACATGTATTTTAT+GGG - chr3_1:88748405-88748424 Msa0385250:intron 20.0%
!! TACTTGTATATATCTAAACA+GGG + chr3_1:88748672-88748691 None:intergenic 20.0%
!!! TTTTGATTTAGTTTTTCTTC+TGG + chr3_1:88748739-88748758 None:intergenic 20.0%
!!! TTTATTTCTAACTCTCTTTT+GGG + chr3_1:88748793-88748812 None:intergenic 20.0%
!!! TTTTATTTCTAACTCTCTTT+TGG + chr3_1:88748794-88748813 None:intergenic 20.0%
!! TTTCAGAAGAAGAAAAAAAA+AGG - chr3_1:88749622-88749641 Msa0385250:CDS 20.0%
!! TTCAGAAGAAGAAAAAAAAA+GGG - chr3_1:88749623-88749642 Msa0385250:CDS 20.0%
!! CACATAATATTTAACTCTTT+AGG + chr3_1:88750358-88750377 None:intergenic 20.0%
! GTCATTGAAATTGTGTAATT+TGG - chr3_1:88748178-88748197 Msa0385250:three_prime_UTR 25.0%
!!! TTAATTTTGCTACTTCGTTA+TGG - chr3_1:88748288-88748307 Msa0385250:three_prime_UTR 25.0%
!!! ATAACGTGTTTTAAATTCCT+TGG + chr3_1:88748367-88748386 None:intergenic 25.0%
! TAATAAGTTTGATTACCTCA+AGG + chr3_1:88748442-88748461 None:intergenic 25.0%
! CCTAATATGCAATACATTAA+GGG + chr3_1:88748598-88748617 None:intergenic 25.0%
! TCCTAATATGCAATACATTA+AGG + chr3_1:88748599-88748618 None:intergenic 25.0%
! GTACTTGTATATATCTAAAC+AGG + chr3_1:88748673-88748692 None:intergenic 25.0%
! ATGCTATAAACTGCAAATTT+AGG + chr3_1:88748695-88748714 None:intergenic 25.0%
!! TTGGTAGATTTTCATCAATT+TGG - chr3_1:88749271-88749290 Msa0385250:intron 25.0%
!!! TGTGATGCTGTTTTAATTTT+TGG - chr3_1:88749445-88749464 Msa0385250:intron 25.0%
!!! TTAACCATATGTTTTAAGAG+AGG + chr3_1:88749684-88749703 None:intergenic 25.0%
!! ACAAAAACAGTTTTTCTTTG+TGG + chr3_1:88749918-88749937 None:intergenic 25.0%
! TATAAGCTGTTAAGTTATTC+TGG - chr3_1:88749937-88749956 Msa0385250:intron 25.0%
!! TTTTCACGTTTTATATGCAT+TGG - chr3_1:88750119-88750138 Msa0385250:intron 25.0%
!! TTTCACGTTTTATATGCATT+GGG - chr3_1:88750120-88750139 Msa0385250:intron 25.0%
! ACATAACAACTTCAAAACAT+TGG + chr3_1:88750301-88750320 None:intergenic 25.0%
! AAAACACAACCAAAATATTG+TGG + chr3_1:88750333-88750352 None:intergenic 25.0%
! AGATCTATCTATAAAAGAAG+TGG + chr3_1:88750618-88750637 None:intergenic 25.0%
!! ATTCAGTGTTGTGAATTTTA+AGG + chr3_1:88750720-88750739 None:intergenic 25.0%
GTAAATGATGATTCATGATC+CGG - chr3_1:88748098-88748117 Msa0385250:three_prime_UTR 30.0%
TAAATGATGATTCATGATCC+GGG - chr3_1:88748099-88748118 Msa0385250:three_prime_UTR 30.0%
TGATTAAATTACTGGAAACC+CGG + chr3_1:88748120-88748139 None:intergenic 30.0%
! AATTTCATTTGAGGTTGTGA+GGG - chr3_1:88748139-88748158 Msa0385250:three_prime_UTR 30.0%
TTAAAACACGTTATTGAGCT+TGG - chr3_1:88748373-88748392 Msa0385250:CDS 30.0%
!!! TGGGTTATTTTTTTTCCTTG+AGG - chr3_1:88748424-88748443 Msa0385250:intron 30.0%
CCCTTAATGTATTGCATATT+AGG - chr3_1:88748595-88748614 Msa0385250:intron 30.0%
TAGCATAAGTACATAGCATA+AGG - chr3_1:88748709-88748728 Msa0385250:intron 30.0%
!!! GATTTAGTTTTTCTTCTGGT+GGG + chr3_1:88748735-88748754 None:intergenic 30.0%
!!! TGATTTAGTTTTTCTTCTGG+TGG + chr3_1:88748736-88748755 None:intergenic 30.0%
AAACTAAATCAAAATCCCCT+TGG - chr3_1:88748745-88748764 Msa0385250:intron 30.0%
AACTAAATCAAAATCCCCTT+GGG - chr3_1:88748746-88748765 Msa0385250:intron 30.0%
GTTAGAAATAAAAGCAGCAA+TGG - chr3_1:88748801-88748820 Msa0385250:intron 30.0%
ATGGACATAAACATTCTACA+AGG - chr3_1:88748820-88748839 Msa0385250:intron 30.0%
TGGACATAAACATTCTACAA+GGG - chr3_1:88748821-88748840 Msa0385250:intron 30.0%
TACAAGGGACCTAATTTATA+TGG - chr3_1:88748836-88748855 Msa0385250:intron 30.0%
!! TTGAGAAAATAGTTTGCTGT+AGG - chr3_1:88749075-88749094 Msa0385250:intron 30.0%
!! TGAGAAAATAGTTTGCTGTA+GGG - chr3_1:88749076-88749095 Msa0385250:intron 30.0%
TTGATGAAAATCTACCAACA+AGG + chr3_1:88749269-88749288 None:intergenic 30.0%
! TTTTCTTTCTTGGGCTTTTA+GGG - chr3_1:88749320-88749339 Msa0385250:intron 30.0%
CTTACAAACAAATAACCAAG+AGG + chr3_1:88749476-88749495 None:intergenic 30.0%
CTCTTGGTTATTTGTTTGTA+AGG - chr3_1:88749474-88749493 Msa0385250:intron 30.0%
TATTTGTTTGTAAGGTTGTG+TGG - chr3_1:88749482-88749501 Msa0385250:intron 30.0%
TTAGCCTCTCTTAAAACATA+TGG - chr3_1:88749677-88749696 Msa0385250:CDS 30.0%
TTCATGTTCTGTGATTAAAG+CGG + chr3_1:88749877-88749896 None:intergenic 30.0%
GCTTATTTGAACTTATTACC+TGG + chr3_1:88750052-88750071 None:intergenic 30.0%
TCAAATAAGCCAATCCAAAT+AGG - chr3_1:88750062-88750081 Msa0385250:intron 30.0%
CAAATAAGCCAATCCAAATA+GGG - chr3_1:88750063-88750082 Msa0385250:intron 30.0%
TTGTAGGTATTACTGCAAAA+TGG + chr3_1:88750212-88750231 None:intergenic 30.0%
ACAACCAAAATATTGTGGAT+AGG + chr3_1:88750328-88750347 None:intergenic 30.0%
AATTGGAAGCAATGGATATT+TGG + chr3_1:88750690-88750709 None:intergenic 30.0%
GAGAAATAAATTGGAAGCAA+TGG + chr3_1:88750698-88750717 None:intergenic 30.0%
AAAAAACGATGATCTATCGG+AGG - chr3_1:88747900-88747919 Msa0385250:three_prime_UTR 35.0%
GACCAATATGAAGTTTGCAA+CGG + chr3_1:88747981-88748000 None:intergenic 35.0%
ATGAATCAACCGCATAAAAG+GGG + chr3_1:88748038-88748057 None:intergenic 35.0%
GATGAATCAACCGCATAAAA+GGG + chr3_1:88748039-88748058 None:intergenic 35.0%
! CAATTTCATTTGAGGTTGTG+AGG - chr3_1:88748138-88748157 Msa0385250:three_prime_UTR 35.0%
GCTACTTCGTTATGGATTTA+TGG - chr3_1:88748296-88748315 Msa0385250:three_prime_UTR 35.0%
!! ACGTGTTTTAAATTCCTTGG+AGG + chr3_1:88748364-88748383 None:intergenic 35.0%
!! TTAGGATTAGTCGCTAAGAT+AGG - chr3_1:88748613-88748632 Msa0385250:intron 35.0%
! AAGAGAACCGTGTTTGATTT+AGG - chr3_1:88748646-88748665 Msa0385250:intron 35.0%
CCTCTTACGCCATATAAATT+AGG + chr3_1:88748848-88748867 None:intergenic 35.0%
CCTAATTTATATGGCGTAAG+AGG - chr3_1:88748845-88748864 Msa0385250:intron 35.0%
TTATATGGCGTAAGAGGATT+CGG - chr3_1:88748851-88748870 Msa0385250:intron 35.0%
! AGGATATTAAAGTGCTATCG+TGG - chr3_1:88748977-88748996 Msa0385250:intron 35.0%
TTTCTCAACAGGCAATCATT+CGG + chr3_1:88749063-88749082 None:intergenic 35.0%
! CAGCAAACTATTTTCTCAAC+AGG + chr3_1:88749074-88749093 None:intergenic 35.0%
!! GAGAAAATAGTTTGCTGTAG+GGG - chr3_1:88749077-88749096 Msa0385250:intron 35.0%
! GTTTTCTTTCTTGGGCTTTT+AGG - chr3_1:88749319-88749338 Msa0385250:intron 35.0%
TCACAACAGTACAAATTGTG+AGG + chr3_1:88749430-88749449 None:intergenic 35.0%
!!! TGCTGTTTTAATTTTTGGCC+TGG - chr3_1:88749450-88749469 Msa0385250:intron 35.0%
TTGTAAGGTTGTGTGGTTTA+AGG - chr3_1:88749489-88749508 Msa0385250:intron 35.0%
!! GTGGTTTAAGGTGTTTTAGA+AGG - chr3_1:88749501-88749520 Msa0385250:intron 35.0%
ACGGATACTCTAGCTAAATT+AGG + chr3_1:88749650-88749669 None:intergenic 35.0%
ATGGCATTAATTGCTAACAG+AGG - chr3_1:88749798-88749817 Msa0385250:intron 35.0%
TACAACTTGCAAAGGAGAAA+GGG + chr3_1:88749830-88749849 None:intergenic 35.0%
TCATGTTCTGTGATTAAAGC+GGG + chr3_1:88749876-88749895 None:intergenic 35.0%
! TGTGGCTTTGCAGTAAATTT+GGG + chr3_1:88749900-88749919 None:intergenic 35.0%
! TTGTGGCTTTGCAGTAAATT+TGG + chr3_1:88749901-88749920 None:intergenic 35.0%
TCACTAATAGTGTGTTTGGT+CGG + chr3_1:88750170-88750189 None:intergenic 35.0%
AAAATGGATAGTCCTTGCAT+TGG + chr3_1:88750196-88750215 None:intergenic 35.0%
AGCAGGATAACAGTTATTGT+AGG + chr3_1:88750228-88750247 None:intergenic 35.0%
ACAATAACTGTTATCCTGCT+AGG - chr3_1:88750228-88750247 Msa0385250:intron 35.0%
AACAATAACAGTTCGTCGAA+TGG - chr3_1:88750267-88750286 Msa0385250:intron 35.0%
! CTCTCCTATCCACAATATTT+TGG - chr3_1:88750321-88750340 Msa0385250:intron 35.0%
! CGTCTTTTATTGTACTTTCC+AGG - chr3_1:88750389-88750408 Msa0385250:intron 35.0%
GTGACATATTTCCTACTACT+TGG + chr3_1:88750463-88750482 None:intergenic 35.0%
TAGTAGGAAATATGTCACAG+TGG - chr3_1:88750465-88750484 Msa0385250:intron 35.0%
TAACTGTTGTCTTGCATTTC+AGG + chr3_1:88750655-88750674 None:intergenic 35.0%
GACAACAGTTAAACAAAGAC+AGG - chr3_1:88750664-88750683 Msa0385250:intron 35.0%
! TCTCATTCACCCCTTTTATG+CGG - chr3_1:88748026-88748045 Msa0385250:three_prime_UTR 40.0%
GGATGAATCAACCGCATAAA+AGG + chr3_1:88748040-88748059 None:intergenic 40.0%
TCCGAATGAAGCTGAAATTC+TGG - chr3_1:88748255-88748274 Msa0385250:three_prime_UTR 40.0%
CCCAGAATTTCAGCTTCATT+CGG + chr3_1:88748259-88748278 None:intergenic 40.0%
CCGAATGAAGCTGAAATTCT+GGG - chr3_1:88748256-88748275 Msa0385250:three_prime_UTR 40.0%
TTTGATTGCTTCCTAACGCT+AGG - chr3_1:88748331-88748350 Msa0385250:three_prime_UTR 40.0%
ACACGTTATTGAGCTTGGTT+TGG - chr3_1:88748378-88748397 Msa0385250:CDS 40.0%
CACGTTATTGAGCTTGGTTT+GGG - chr3_1:88748379-88748398 Msa0385250:CDS 40.0%
AACAGGGCCTAAATCAAACA+CGG + chr3_1:88748656-88748675 None:intergenic 40.0%
AAATCAAAATCCCCTTGGGA+AGG - chr3_1:88748750-88748769 Msa0385250:intron 40.0%
TCACTTTGTAACCTTCCCAA+GGG + chr3_1:88748764-88748783 None:intergenic 40.0%
TTATATGGCACGCAACGATT+TGG - chr3_1:88748887-88748906 Msa0385250:intron 40.0%
TATATGGCACGCAACGATTT+GGG - chr3_1:88748888-88748907 Msa0385250:intron 40.0%
! TTTGGGGTATTTAGCAAGCT+AGG - chr3_1:88748905-88748924 Msa0385250:intron 40.0%
CAACAATGAGTCGAAAGATG+TGG - chr3_1:88748942-88748961 Msa0385250:intron 40.0%
GAAAGATGTGGAGTCTATTG+TGG - chr3_1:88748954-88748973 Msa0385250:intron 40.0%
CAACTTGTCTGTACTACGAA+TGG - chr3_1:88749028-88749047 Msa0385250:intron 40.0%
!!! CTGTAGGGGTTTTTTGATGT+AGG - chr3_1:88749091-88749110 Msa0385250:intron 40.0%
!!! GTTTTTTGATGTAGGTGCCT+TGG - chr3_1:88749099-88749118 Msa0385250:intron 40.0%
AATCCCTTCTGCTACTCATT+TGG - chr3_1:88749124-88749143 Msa0385250:intron 40.0%
!! CGTTTTTGTGTTGGTATCCT+GGG - chr3_1:88749222-88749241 Msa0385250:intron 40.0%
!! GTTTTTGTGTTGGTATCCTG+GGG - chr3_1:88749223-88749242 Msa0385250:intron 40.0%
!! TCAATTTGGTGCTGATAGTG+AGG - chr3_1:88749285-88749304 Msa0385250:intron 40.0%
! GGGTATGCGTTTTCTTTCTT+GGG - chr3_1:88749311-88749330 Msa0385250:intron 40.0%
AACAGTACAAATTGTGAGGC+TGG + chr3_1:88749426-88749445 None:intergenic 40.0%
AAACAAATAACCAAGAGGCC+AGG + chr3_1:88749471-88749490 None:intergenic 40.0%
!!! GCCTTGGTTTTAGCCATTTT+TGG - chr3_1:88749543-88749562 Msa0385250:CDS 40.0%
GCCAAAAATGGCTAAAACCA+AGG + chr3_1:88749547-88749566 None:intergenic 40.0%
TTGAGGTCTTGAGGATTTGA+TGG + chr3_1:88749728-88749747 None:intergenic 40.0%
GCAATTAATGCCATCAACTC+AGG + chr3_1:88749792-88749811 None:intergenic 40.0%
CTACAACTTGCAAAGGAGAA+AGG + chr3_1:88749831-88749850 None:intergenic 40.0%
TTGGAGTCTACAACTTGCAA+AGG + chr3_1:88749838-88749857 None:intergenic 40.0%
CATGTTCTGTGATTAAAGCG+GGG + chr3_1:88749875-88749894 None:intergenic 40.0%
AACTGAGTCCCTATTTGGAT+TGG + chr3_1:88750074-88750093 None:intergenic 40.0%
TCAAGAACTGAGTCCCTATT+TGG + chr3_1:88750079-88750098 None:intergenic 40.0%
CACTAATAGTGTGTTTGGTC+GGG + chr3_1:88750169-88750188 None:intergenic 40.0%
GACCAAACACACTATTAGTG+AGG - chr3_1:88750169-88750188 Msa0385250:intron 40.0%
GACCTCACTAATAGTGTGTT+TGG + chr3_1:88750174-88750193 None:intergenic 40.0%
TATTAGTGAGGTCCAATGCA+AGG - chr3_1:88750181-88750200 Msa0385250:intron 40.0%
ATTTCCTACTACTTGGTTGC+AGG + chr3_1:88750456-88750475 None:intergenic 40.0%
AAACGATGATCTATCGGAGG+TGG - chr3_1:88747903-88747922 Msa0385250:three_prime_UTR 45.0%
CGCCGTTGCAAACTTCATAT+TGG - chr3_1:88747976-88747995 Msa0385250:three_prime_UTR 45.0%
! TCATTTGAGGTTGTGAGGGT+TGG - chr3_1:88748143-88748162 Msa0385250:three_prime_UTR 45.0%
GAGGAGAAAATCCTAGCGTT+AGG + chr3_1:88748345-88748364 None:intergenic 45.0%
!! GGATTAGTCGCTAAGATAGG+AGG - chr3_1:88748616-88748635 Msa0385250:intron 45.0%
CACTTTGTAACCTTCCCAAG+GGG + chr3_1:88748763-88748782 None:intergenic 45.0%
CTCACTTTGTAACCTTCCCA+AGG + chr3_1:88748765-88748784 None:intergenic 45.0%
GGAGAGGTCTTAGGCTTATA+TGG - chr3_1:88748872-88748891 Msa0385250:intron 45.0%
ATATGGCACGCAACGATTTG+GGG - chr3_1:88748889-88748908 Msa0385250:intron 45.0%
AGATGTGGAGTCTATTGTGG+AGG - chr3_1:88748957-88748976 Msa0385250:intron 45.0%
! TTAAAGTGCTATCGTGGCTG+TGG - chr3_1:88748983-88749002 Msa0385250:intron 45.0%
GTCTGTACTACGAATGGAGT+TGG - chr3_1:88749034-88749053 Msa0385250:intron 45.0%
GTAGCAGAAGGGATTAACCA+AGG + chr3_1:88749119-88749138 None:intergenic 45.0%
GCTCCAAATGAGTAGCAGAA+GGG + chr3_1:88749130-88749149 None:intergenic 45.0%
CTTCTGCTACTCATTTGGAG+CGG - chr3_1:88749129-88749148 Msa0385250:intron 45.0%
CAGCACAGACAAGCCAAAAA+AGG + chr3_1:88749185-88749204 None:intergenic 45.0%
!! GCGTTTTTGTGTTGGTATCC+TGG - chr3_1:88749221-88749240 Msa0385250:intron 45.0%
! GGGGTATGCGTTTTCTTTCT+TGG - chr3_1:88749310-88749329 Msa0385250:intron 45.0%
! GGAGCAGTAGCTAGTTTCTT+GGG - chr3_1:88749341-88749360 Msa0385250:intron 45.0%
!!! TAATTTTTGGCCTGGCCTCT+TGG - chr3_1:88749458-88749477 Msa0385250:intron 45.0%
!! GAAGGTGTTCCGCCTATATA+CGG - chr3_1:88749519-88749538 Msa0385250:CDS 45.0%
!! AAAACCAAGGCGCCGTATAT+AGG + chr3_1:88749534-88749553 None:intergenic 45.0%
! TAGCTAGAGTATCCGTCACT+TGG - chr3_1:88749654-88749673 Msa0385250:CDS 45.0%
AAGAGAGGCTAACCAAGTGA+CGG + chr3_1:88749669-88749688 None:intergenic 45.0%
TGTGATTAAAGCGGGGCTTT+GGG + chr3_1:88749868-88749887 None:intergenic 45.0%
TCTAAGCAATCTCACCTAGC+AGG + chr3_1:88750245-88750264 None:intergenic 45.0%
!! TCTCGGCTGGTTTGGTAAAT+GGG + chr3_1:88750416-88750435 None:intergenic 45.0%
TTGGTCACCAAACTTCCTCT+CGG + chr3_1:88750433-88750452 None:intergenic 45.0%
ACTAGCGAACATGTCTTGCA+AGG + chr3_1:88750541-88750560 None:intergenic 45.0%
GTCGAATGAAGTCTAGACTC+TGG - chr3_1:88750577-88750596 Msa0385250:intron 45.0%
!! GAAGTCTAGACTCTGGAGTA+TGG - chr3_1:88750584-88750603 Msa0385250:intron 45.0%
! CGCTAGGATTTTCTCCTCCA+AGG - chr3_1:88748347-88748366 Msa0385250:exon 50.0%
AGAGGATTCGGAGAGGTCTT+AGG - chr3_1:88748863-88748882 Msa0385250:intron 50.0%
CGCTCCAAATGAGTAGCAGA+AGG + chr3_1:88749131-88749150 None:intergenic 50.0%
GCTGTCATAGTGATCGGTTG+TGG - chr3_1:88749154-88749173 Msa0385250:intron 50.0%
GTCATAGTGATCGGTTGTGG+AGG - chr3_1:88749157-88749176 Msa0385250:intron 50.0%
!!! GGTTGTGGAGGAGCCTTTTT+TGG - chr3_1:88749169-88749188 Msa0385250:intron 50.0%
!!! TGTGCTGCTGTTTTCTGCTG+TGG - chr3_1:88749198-88749217 Msa0385250:intron 50.0%
!!! GTGCTGCTGTTTTCTGCTGT+GGG - chr3_1:88749199-88749218 Msa0385250:intron 50.0%
! TGCTGTGGGCGTTTTTGTGT+TGG - chr3_1:88749213-88749232 Msa0385250:intron 50.0%
!! TGGAAGTGTGGTAGCCTTGT+TGG - chr3_1:88749252-88749271 Msa0385250:intron 50.0%
!! TTTGGTGCTGATAGTGAGGC+AGG - chr3_1:88749289-88749308 Msa0385250:intron 50.0%
!! TTGGTGCTGATAGTGAGGCA+GGG - chr3_1:88749290-88749309 Msa0385250:intron 50.0%
! GGGAGCAGTAGCTAGTTTCT+TGG - chr3_1:88749340-88749359 Msa0385250:intron 50.0%
GCAAGACGCACAAAAACTCG+CGG + chr3_1:88749575-88749594 None:intergenic 50.0%
TCAGGATGGTTGAGGTCTTG+AGG + chr3_1:88749737-88749756 None:intergenic 50.0%
! CAACACACGACCTGAGTTGA+TGG - chr3_1:88749779-88749798 Msa0385250:intron 50.0%
CTGTGATTAAAGCGGGGCTT+TGG + chr3_1:88749869-88749888 None:intergenic 50.0%
! TACCTGGCAAAGCACTTGTG+AGG + chr3_1:88750036-88750055 None:intergenic 50.0%
!! CTGGTTTGGTAAATGGGTCC+TGG + chr3_1:88750410-88750429 None:intergenic 50.0%
!! CTCTCGGCTGGTTTGGTAAA+TGG + chr3_1:88750417-88750436 None:intergenic 50.0%
CATTTACCAAACCAGCCGAG+AGG - chr3_1:88750415-88750434 Msa0385250:intron 50.0%
! AAACTTCCTCTCGGCTGGTT+TGG + chr3_1:88750424-88750443 None:intergenic 50.0%
AAACCAGCCGAGAGGAAGTT+TGG - chr3_1:88750423-88750442 Msa0385250:intron 50.0%
CCTACTACTTGGTTGCAGGT+TGG + chr3_1:88750452-88750471 None:intergenic 50.0%
CCAACCTGCAACCAAGTAGT+AGG - chr3_1:88750449-88750468 Msa0385250:intron 50.0%
! GTCTTGCAAGGAGGCATACA+TGG + chr3_1:88750529-88750548 None:intergenic 50.0%
AGCGAACATGTCTTGCAAGG+AGG + chr3_1:88750538-88750557 None:intergenic 50.0%
! GTTAAGCTGCTAGCTTACCG+TGG - chr3_1:88750960-88750979 Msa0385250:five_prime_UTR 50.0%
GAGTCTCCTTACAGGTCCGA+CGG - chr3_1:88747925-88747944 Msa0385250:three_prime_UTR 55.0%
GAAGTTTGCAACGGCGACAG+TGG + chr3_1:88747972-88747991 None:intergenic 55.0%
TGGCGTAAGAGGATTCGGAG+AGG - chr3_1:88748856-88748875 Msa0385250:intron 55.0%
GCGGCTGCTGTCATAGTGAT+CGG - chr3_1:88749148-88749167 Msa0385250:intron 55.0%
TTGGTATCCTGGGGAGTTGC+TGG - chr3_1:88749232-88749251 Msa0385250:intron 55.0%
!! TGGTGCTGATAGTGAGGCAG+GGG - chr3_1:88749291-88749310 Msa0385250:intron 55.0%
!! ACCAAGGCGCCGTATATAGG+CGG + chr3_1:88749531-88749550 None:intergenic 55.0%
CTCGCGGAAGGAGCCAAAAA+TGG + chr3_1:88749559-88749578 None:intergenic 55.0%
GACGCACAAAAACTCGCGGA+AGG + chr3_1:88749571-88749590 None:intergenic 55.0%
!! CGGTTTGGTCAGGATGGTTG+AGG + chr3_1:88749745-88749764 None:intergenic 55.0%
!! TTGTCGCGGTTTGGTCAGGA+TGG + chr3_1:88749751-88749770 None:intergenic 55.0%
AGCCTCACAAGTGCTTTGCC+AGG - chr3_1:88750031-88750050 Msa0385250:intron 55.0%
TCACCAAACTTCCTCTCGGC+TGG + chr3_1:88750429-88750448 None:intergenic 55.0%
CGGAGGTGGAGTCTCCTTAC+AGG - chr3_1:88747917-88747936 Msa0385250:three_prime_UTR 60.0%
CACACTTCCAGCAACTCCCC+AGG + chr3_1:88749242-88749261 None:intergenic 60.0%
CTGGGGAGTTGCTGGAAGTG+TGG - chr3_1:88749240-88749259 Msa0385250:intron 60.0%
TCCGCCTATATACGGCGCCT+TGG - chr3_1:88749527-88749546 Msa0385250:CDS 60.0%
!! ACGCTTGTCGCGGTTTGGTC+AGG + chr3_1:88749755-88749774 None:intergenic 60.0%
TGACCAAACCGCGACAAGCG+TGG - chr3_1:88749754-88749773 Msa0385250:intron 60.0%
GACCAAACCGCGACAAGCGT+GGG - chr3_1:88749755-88749774 Msa0385250:intron 60.0%
! GTGTTGACCCACGCTTGTCG+CGG + chr3_1:88749765-88749784 None:intergenic 60.0%
!! CGGGGCTTTGGGCAGTTGTT+TGG + chr3_1:88749857-88749876 None:intergenic 60.0%
CGAGAGCCGTCGGACCTGTA+AGG + chr3_1:88747934-88747953 None:intergenic 65.0%
ACACCAGCGACGAGAGCCGT+CGG + chr3_1:88747944-88747963 None:intergenic 65.0%
GACCCACGCTTGTCGCGGTT+TGG + chr3_1:88749760-88749779 None:intergenic 65.0%
GACGGCTCTCGTCGCTGGTG+TGG - chr3_1:88747943-88747962 Msa0385250:three_prime_UTR 70.0%
GGCTCTCGTCGCTGGTGTGG+TGG - chr3_1:88747946-88747965 Msa0385250:three_prime_UTR 70.0%
GGTCCGACGGCTCTCGTCGC+TGG - chr3_1:88747938-88747957 Msa0385250:three_prime_UTR 75.0%
Chromosome Type Strat End Strand Name
chr3_1 gene 88747844 88750983 88747844 ID=Msa0385250;Name=Msa0385250
chr3_1 mRNA 88747844 88750983 88747844 ID=Msa0385250-mRNA-1;Parent=Msa0385250;Name=Msa0385250-mRNA-1;_AED=0.04;_eAED=0.04;_QI=64|0|0.66|1|1|1|3|516|123
chr3_1 exon 88747844 88748416 88747844 ID=Msa0385250-mRNA-1:exon:23989;Parent=Msa0385250-mRNA-1
chr3_1 exon 88749515 88749730 88749515 ID=Msa0385250-mRNA-1:exon:23988;Parent=Msa0385250-mRNA-1
chr3_1 exon 88750821 88750983 88750821 ID=Msa0385250-mRNA-1:exon:23987;Parent=Msa0385250-mRNA-1
chr3_1 five_prime_UTR 88750920 88750983 88750920 ID=Msa0385250-mRNA-1:five_prime_utr;Parent=Msa0385250-mRNA-1
chr3_1 CDS 88750821 88750919 88750821 ID=Msa0385250-mRNA-1:cds;Parent=Msa0385250-mRNA-1
chr3_1 CDS 88749515 88749730 88749515 ID=Msa0385250-mRNA-1:cds;Parent=Msa0385250-mRNA-1
chr3_1 CDS 88748360 88748416 88748360 ID=Msa0385250-mRNA-1:cds;Parent=Msa0385250-mRNA-1
chr3_1 three_prime_UTR 88747844 88748359 88747844 ID=Msa0385250-mRNA-1:three_prime_utr;Parent=Msa0385250-mRNA-1
Gene Sequence

>Msa0385250

ATGATCTATCGGAGGTGGAGTCTCCTTACAGGTCCGACGGCTCTCGTCGCTGGTGTGGTGGCCACTGTCGCCGTTGCAAACTTCATATTGGTCAAGAATGGGTTTTTTGATGTAGGTGCCTTGGTTAATCCCTTCTGCTACTCATTTGGAGCGGCTGCTGTCATAGTGATCGGTTGTGGAGGAGCCTTTTTTGGCTTGTCTGTGCTGCTGTTTTCTGCTGTGGGCGTTTTTGTGTTGGTATCCTGGGGAGTTGCTGGAAGTGTGGTAGCCTTGTTGGTAGATTTTCATCAATTTGGTGCTGATAGTGAGGCAGGGGACCCATTTACCAAACCAGCCGAGAGGAAGTTTGGTGACCAACCTGCAACCAAGTAG
Protein sequence

>Msa0385250

MIYRRWSLLTGPTALVAGVVATVAVANFILVKNGFFDVGALVNPFCYSFGAAAVIVIGCGGAFFGLSVLLFSAVGVFVLVSWGVAGSVVALLVDFHQFGADSEAGDPFTKPAERKFGDQPATK*