AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa1123500


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa1123500 AT3G12587.1 81.081 37 7 0 41 77 1 37 1.20e-16 66.6
Msa1123500 AT3G09455.2 80.000 35 7 0 41 75 1 35 9.44e-16 64.3
Msa1123500 AT3G09455.1 80.000 35 7 0 41 75 1 35 9.44e-16 64.3
Msa1123500 AT5G02502.1 77.143 35 8 0 41 75 1 35 2.17e-15 63.5

Find 20 sgRNAs with CRISPR-Local

Find 123 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
TATAATTATGAACCTCGTTT+TGG 0.249507 7_3:-74368469 Msa1123500:three_prime_UTR
TCTAGTTTCCTTCATACTTT+GGG 0.296845 7_3:+74368566 None:intergenic
ATGTTGTGGTCGTGCTTCTT+AGG 0.309733 7_3:+74369663 None:intergenic
TTCTAGTTTCCTTCATACTT+TGG 0.311802 7_3:+74368565 None:intergenic
GATGAAGACGCCGAGAAAAT+TGG 0.325577 7_3:+74368627 None:intergenic
CCTTGAAAACGCGGTGGAAA+TGG 0.326898 7_3:+74369622 None:intergenic
CGGCGTCTTCATCTTTGTGC+TGG 0.380443 7_3:-74368617 Msa1123500:CDS
GATGATTGATGACAATGATC+TGG 0.432151 7_3:-74368659 Msa1123500:CDS
CTTGAAAACGCGGTGGAAAT+GGG 0.434606 7_3:+74369623 None:intergenic
GGAAATGGGTGTTTCTTCTC+CGG 0.476260 7_3:+74369637 None:intergenic
TGCTTATGTGTGCTTAAGAC+CGG 0.487149 7_3:-74368371 None:intergenic
TTCTTCTCCGGCGAATGTTG+TGG 0.493038 7_3:+74369649 None:intergenic
GTGGCTGACCCAAAGTATGA+AGG 0.567065 7_3:-74368574 Msa1123500:CDS
AGACTGAGATGCTTTACAGA+TGG 0.571757 7_3:-74368504 Msa1123500:three_prime_UTR
ATGATTGATGACAATGATCT+GGG 0.577930 7_3:-74368658 Msa1123500:CDS
AGCACGACCACAACATTCGC+CGG 0.611769 7_3:-74369656 Msa1123500:CDS
ATGATACCTTGAAAACGCGG+TGG 0.684438 7_3:+74369616 None:intergenic
AAATTAATATTCCCAAAACG+AGG 0.696429 7_3:+74368457 None:intergenic
GCGATGATACCTTGAAAACG+CGG 0.740904 7_3:+74369613 None:intergenic
GATAGCATATCACTATGTCG+TGG 0.751623 7_3:-74368593 Msa1123500:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! ATAAAATAAAATAATGATGA+TGG + chr7_3:74368551-74368570 None:intergenic 10.0%
!! TCTATATTACTATAAAAATT+GGG - chr7_3:74369188-74369207 Msa1123500:intron 10.0%
!! ATAACAAAACAAAAAAAAAA+TGG + chr7_3:74369702-74369721 None:intergenic 10.0%
!! ATAAAATAAAATAATGATGA+TGG + chr7_3:74368551-74368570 None:intergenic 10.0%
!! TCTATATTACTATAAAAATT+GGG - chr7_3:74369188-74369207 Msa1123500:intron 10.0%
!! ATAACAAAACAAAAAAAAAA+TGG + chr7_3:74369702-74369721 None:intergenic 10.0%
!! GTCTATATTACTATAAAAAT+TGG - chr7_3:74369187-74369206 Msa1123500:intron 15.0%
!! GTCTATATTACTATAAAAAT+TGG - chr7_3:74369187-74369206 Msa1123500:intron 15.0%
!!! CCTAAATTTTTCAGATAATT+CGG - chr7_3:74368719-74368738 Msa1123500:intron 20.0%
!!! GTTTTGATTTTAAGTTGTAA+AGG - chr7_3:74368786-74368805 Msa1123500:intron 20.0%
!!! AAAGGATTTAAGATTTGATT+AGG - chr7_3:74368804-74368823 Msa1123500:intron 20.0%
!! GAGAAAATATATGTTACAAT+TGG - chr7_3:74368854-74368873 Msa1123500:intron 20.0%
!! TAAAAATTGTTGACAAATGT+TGG - chr7_3:74368887-74368906 Msa1123500:intron 20.0%
!!! GAAAATCAATCTACTTTTTT+AGG + chr7_3:74369342-74369361 None:intergenic 20.0%
!!! CCTAAATTTTTCAGATAATT+CGG - chr7_3:74368719-74368738 Msa1123500:intron 20.0%
!!! GTTTTGATTTTAAGTTGTAA+AGG - chr7_3:74368786-74368805 Msa1123500:intron 20.0%
!!! AAAGGATTTAAGATTTGATT+AGG - chr7_3:74368804-74368823 Msa1123500:intron 20.0%
!! GAGAAAATATATGTTACAAT+TGG - chr7_3:74368854-74368873 Msa1123500:intron 20.0%
!! TAAAAATTGTTGACAAATGT+TGG - chr7_3:74368887-74368906 Msa1123500:intron 20.0%
!!! GAAAATCAATCTACTTTTTT+AGG + chr7_3:74369342-74369361 None:intergenic 20.0%
! CCGAATTATCTGAAAAATTT+AGG + chr7_3:74368722-74368741 None:intergenic 25.0%
!! TTTGTCAACAATTTTTAACC+AGG + chr7_3:74368884-74368903 None:intergenic 25.0%
!!! TTTAAGTTGTTTTTCGAAAC+AGG - chr7_3:74369164-74369183 Msa1123500:intron 25.0%
!!! TTAAGTTGTTTTTCGAAACA+GGG - chr7_3:74369165-74369184 Msa1123500:intron 25.0%
! TTACTATAAAAATTGGGATC+AGG - chr7_3:74369194-74369213 Msa1123500:intron 25.0%
! TACTATAAAAATTGGGATCA+GGG - chr7_3:74369195-74369214 Msa1123500:intron 25.0%
!! TATAATTATGAACCTCGTTT+TGG - chr7_3:74369623-74369642 Msa1123500:CDS 25.0%
!! ATAATTATGAACCTCGTTTT+GGG - chr7_3:74369624-74369643 Msa1123500:CDS 25.0%
! AAATTAATATTCCCAAAACG+AGG + chr7_3:74369638-74369657 None:intergenic 25.0%
!!! TGGGAATATTAATTTTAGCT+TGG - chr7_3:74369643-74369662 Msa1123500:CDS 25.0%
! CCGAATTATCTGAAAAATTT+AGG + chr7_3:74368722-74368741 None:intergenic 25.0%
!! TTTGTCAACAATTTTTAACC+AGG + chr7_3:74368884-74368903 None:intergenic 25.0%
!!! TTTAAGTTGTTTTTCGAAAC+AGG - chr7_3:74369164-74369183 Msa1123500:intron 25.0%
!!! TTAAGTTGTTTTTCGAAACA+GGG - chr7_3:74369165-74369184 Msa1123500:intron 25.0%
! TTACTATAAAAATTGGGATC+AGG - chr7_3:74369194-74369213 Msa1123500:intron 25.0%
! TACTATAAAAATTGGGATCA+GGG - chr7_3:74369195-74369214 Msa1123500:intron 25.0%
!! TATAATTATGAACCTCGTTT+TGG - chr7_3:74369623-74369642 Msa1123500:CDS 25.0%
!! ATAATTATGAACCTCGTTTT+GGG - chr7_3:74369624-74369643 Msa1123500:CDS 25.0%
! AAATTAATATTCCCAAAACG+AGG + chr7_3:74369638-74369657 None:intergenic 25.0%
!!! TGGGAATATTAATTTTAGCT+TGG - chr7_3:74369643-74369662 Msa1123500:CDS 25.0%
!! ATTTGCATCAGAGTTTTGTA+AGG - chr7_3:74368391-74368410 Msa1123500:three_prime_UTR 30.0%
! TGATTTACGAGTTTTCAGAT+CGG - chr7_3:74368656-74368675 Msa1123500:CDS 30.0%
TGATAAGAAGTTTAGCTAGT+TGG - chr7_3:74369245-74369264 Msa1123500:intron 30.0%
ATTCAAATGTGCAACAAGAA+GGG + chr7_3:74369279-74369298 None:intergenic 30.0%
AATTCAAATGTGCAACAAGA+AGG + chr7_3:74369280-74369299 None:intergenic 30.0%
TTGTTGCACATTTGAATTTG+TGG - chr7_3:74369282-74369301 Msa1123500:intron 30.0%
! ATTTGAATTTGTGGTCTTGA+TGG - chr7_3:74369291-74369310 Msa1123500:intron 30.0%
! ATTTTGTGATATCACAAACC+AGG - chr7_3:74369375-74369394 Msa1123500:intron 30.0%
! ATGATTGATGACAATGATCT+GGG - chr7_3:74369434-74369453 Msa1123500:intron 30.0%
! TCTAGTTTCCTTCATACTTT+GGG + chr7_3:74369529-74369548 None:intergenic 30.0%
!! TTCTAGTTTCCTTCATACTT+TGG + chr7_3:74369530-74369549 None:intergenic 30.0%
!! ATTTGCATCAGAGTTTTGTA+AGG - chr7_3:74368391-74368410 Msa1123500:three_prime_UTR 30.0%
! TGATTTACGAGTTTTCAGAT+CGG - chr7_3:74368656-74368675 Msa1123500:CDS 30.0%
TGATAAGAAGTTTAGCTAGT+TGG - chr7_3:74369245-74369264 Msa1123500:intron 30.0%
ATTCAAATGTGCAACAAGAA+GGG + chr7_3:74369279-74369298 None:intergenic 30.0%
AATTCAAATGTGCAACAAGA+AGG + chr7_3:74369280-74369299 None:intergenic 30.0%
TTGTTGCACATTTGAATTTG+TGG - chr7_3:74369282-74369301 Msa1123500:intron 30.0%
! ATTTGAATTTGTGGTCTTGA+TGG - chr7_3:74369291-74369310 Msa1123500:intron 30.0%
! ATTTTGTGATATCACAAACC+AGG - chr7_3:74369375-74369394 Msa1123500:intron 30.0%
! ATGATTGATGACAATGATCT+GGG - chr7_3:74369434-74369453 Msa1123500:intron 30.0%
! TCTAGTTTCCTTCATACTTT+GGG + chr7_3:74369529-74369548 None:intergenic 30.0%
!! TTCTAGTTTCCTTCATACTT+TGG + chr7_3:74369530-74369549 None:intergenic 30.0%
AGAAACGAGAATTGAGAAGA+AGG + chr7_3:74368504-74368523 None:intergenic 35.0%
! GATTTACGAGTTTTCAGATC+GGG - chr7_3:74368657-74368676 Msa1123500:CDS 35.0%
! ATTTACGAGTTTTCAGATCG+GGG - chr7_3:74368658-74368677 Msa1123500:CDS 35.0%
TATGTTACAATTGGTAAGCC+TGG - chr7_3:74368863-74368882 Msa1123500:intron 35.0%
TCAGGCTCACAATATATCAA+AGG + chr7_3:74368962-74368981 None:intergenic 35.0%
TGATATATTGTGAGCCTGAT+TGG - chr7_3:74368963-74368982 Msa1123500:intron 35.0%
TCAAACACATCAATCCAATC+AGG + chr7_3:74368980-74368999 None:intergenic 35.0%
TAAGCACTTGTGAAACTGTT+TGG - chr7_3:74369014-74369033 Msa1123500:intron 35.0%
TGGTATGCTTGATTGTCATA+TGG - chr7_3:74369311-74369330 Msa1123500:intron 35.0%
AAAAATATTCGTGAGTGACC+TGG + chr7_3:74369396-74369415 None:intergenic 35.0%
! GATGATTGATGACAATGATC+TGG - chr7_3:74369433-74369452 Msa1123500:intron 35.0%
! GGTTTCTTTGCCAATTTTCT+CGG - chr7_3:74369455-74369474 Msa1123500:intron 35.0%
AGAAACGAGAATTGAGAAGA+AGG + chr7_3:74368504-74368523 None:intergenic 35.0%
! GATTTACGAGTTTTCAGATC+GGG - chr7_3:74368657-74368676 Msa1123500:CDS 35.0%
! ATTTACGAGTTTTCAGATCG+GGG - chr7_3:74368658-74368677 Msa1123500:CDS 35.0%
TATGTTACAATTGGTAAGCC+TGG - chr7_3:74368863-74368882 Msa1123500:intron 35.0%
TCAGGCTCACAATATATCAA+AGG + chr7_3:74368962-74368981 None:intergenic 35.0%
TGATATATTGTGAGCCTGAT+TGG - chr7_3:74368963-74368982 Msa1123500:intron 35.0%
TCAAACACATCAATCCAATC+AGG + chr7_3:74368980-74368999 None:intergenic 35.0%
TAAGCACTTGTGAAACTGTT+TGG - chr7_3:74369014-74369033 Msa1123500:intron 35.0%
TGGTATGCTTGATTGTCATA+TGG - chr7_3:74369311-74369330 Msa1123500:intron 35.0%
AAAAATATTCGTGAGTGACC+TGG + chr7_3:74369396-74369415 None:intergenic 35.0%
! GATGATTGATGACAATGATC+TGG - chr7_3:74369433-74369452 Msa1123500:intron 35.0%
! GGTTTCTTTGCCAATTTTCT+CGG - chr7_3:74369455-74369474 Msa1123500:intron 35.0%
ATGCCGACACTTATTCTTAC+AGG - chr7_3:74368610-74368629 Msa1123500:CDS 40.0%
GCACCTGTAAGAATAAGTGT+CGG + chr7_3:74368616-74368635 None:intergenic 40.0%
CTGTAAATGCAATCCTGATG+TGG - chr7_3:74368918-74368937 Msa1123500:intron 40.0%
AAAATCTCTGCAACCACATC+AGG + chr7_3:74368934-74368953 None:intergenic 40.0%
GATAGCATATCACTATGTCG+TGG - chr7_3:74369499-74369518 Msa1123500:intron 40.0%
AGACTGAGATGCTTTACAGA+TGG - chr7_3:74369588-74369607 Msa1123500:intron 40.0%
TAACTAGCACAAGAGTCTAG+CGG + chr7_3:74368372-74368391 None:intergenic 40.0%
ATGCCGACACTTATTCTTAC+AGG - chr7_3:74368610-74368629 Msa1123500:CDS 40.0%
GCACCTGTAAGAATAAGTGT+CGG + chr7_3:74368616-74368635 None:intergenic 40.0%
CTGTAAATGCAATCCTGATG+TGG - chr7_3:74368918-74368937 Msa1123500:intron 40.0%
AAAATCTCTGCAACCACATC+AGG + chr7_3:74368934-74368953 None:intergenic 40.0%
GATAGCATATCACTATGTCG+TGG - chr7_3:74369499-74369518 Msa1123500:intron 40.0%
AGACTGAGATGCTTTACAGA+TGG - chr7_3:74369588-74369607 Msa1123500:intron 40.0%
ATGTTGTGGTCGTGCTTCTT+AGG + chr7_3:74368432-74368451 None:intergenic 45.0%
GGAAATGGGTGTTTCTTCTC+CGG + chr7_3:74368458-74368477 None:intergenic 45.0%
CTTGAAAACGCGGTGGAAAT+GGG + chr7_3:74368472-74368491 None:intergenic 45.0%
ATGATACCTTGAAAACGCGG+TGG + chr7_3:74368479-74368498 None:intergenic 45.0%
GCGATGATACCTTGAAAACG+CGG + chr7_3:74368482-74368501 None:intergenic 45.0%
GATGAAGACGCCGAGAAAAT+TGG + chr7_3:74369468-74369487 None:intergenic 45.0%
ATGTTGTGGTCGTGCTTCTT+AGG + chr7_3:74368432-74368451 None:intergenic 45.0%
GGAAATGGGTGTTTCTTCTC+CGG + chr7_3:74368458-74368477 None:intergenic 45.0%
CTTGAAAACGCGGTGGAAAT+GGG + chr7_3:74368472-74368491 None:intergenic 45.0%
ATGATACCTTGAAAACGCGG+TGG + chr7_3:74368479-74368498 None:intergenic 45.0%
GCGATGATACCTTGAAAACG+CGG + chr7_3:74368482-74368501 None:intergenic 45.0%
GATGAAGACGCCGAGAAAAT+TGG + chr7_3:74369468-74369487 None:intergenic 45.0%
TTCTTCTCCGGCGAATGTTG+TGG + chr7_3:74368446-74368465 None:intergenic 50.0%
CCTTGAAAACGCGGTGGAAA+TGG + chr7_3:74368473-74368492 None:intergenic 50.0%
! CCATTTCCACCGCGTTTTCA+AGG - chr7_3:74368470-74368489 Msa1123500:three_prime_UTR 50.0%
GTGGCTGACCCAAAGTATGA+AGG - chr7_3:74369518-74369537 Msa1123500:intron 50.0%
TTCTTCTCCGGCGAATGTTG+TGG + chr7_3:74368446-74368465 None:intergenic 50.0%
CCTTGAAAACGCGGTGGAAA+TGG + chr7_3:74368473-74368492 None:intergenic 50.0%
! CCATTTCCACCGCGTTTTCA+AGG - chr7_3:74368470-74368489 Msa1123500:three_prime_UTR 50.0%
GTGGCTGACCCAAAGTATGA+AGG - chr7_3:74369518-74369537 Msa1123500:intron 50.0%
AGCACGACCACAACATTCGC+CGG - chr7_3:74368436-74368455 Msa1123500:three_prime_UTR 55.0%
CGGCGTCTTCATCTTTGTGC+TGG - chr7_3:74369475-74369494 Msa1123500:intron 55.0%
AGCACGACCACAACATTCGC+CGG - chr7_3:74368436-74368455 Msa1123500:three_prime_UTR 55.0%
CGGCGTCTTCATCTTTGTGC+TGG - chr7_3:74369475-74369494 Msa1123500:intron 55.0%
Chromosome Type Strat End Strand Name
chr7_3 gene 74368372 74369742 74368372 ID=Msa1123500;Name=Msa1123500
chr7_3 mRNA 74368372 74369742 74368372 ID=Msa1123500-mRNA-1;Parent=Msa1123500;Name=Msa1123500-mRNA-1;_AED=0.05;_eAED=0.05;_QI=0|1|1|1|1|1|2|195|77
chr7_3 exon 74368372 74368680 74368372 ID=Msa1123500-mRNA-1:exon:19772;Parent=Msa1123500-mRNA-1
chr7_3 exon 74369623 74369742 74369623 ID=Msa1123500-mRNA-1:exon:19771;Parent=Msa1123500-mRNA-1
chr7_3 CDS 74369623 74369742 74369623 ID=Msa1123500-mRNA-1:cds;Parent=Msa1123500-mRNA-1
chr7_3 CDS 74368567 74368680 74368567 ID=Msa1123500-mRNA-1:cds;Parent=Msa1123500-mRNA-1
chr7_3 three_prime_UTR 74368372 74368566 74368372 ID=Msa1123500-mRNA-1:three_prime_utr;Parent=Msa1123500-mRNA-1
Gene Sequence

>Msa1123500

CTAGACTCTTGTGCTAGTTATTTGCATCAGAGTTTTGTAAGGTTATACAGAACGAGACCTAAGAAGCACGACCACAACATTCGCCGGAGAAGAAACACCCATTTCCACCGCGTTTTCAAGATGATTGATGACAATGATCTGGGTTTCTTTGCCAATTTTCTCGGCGTCTTCATCTTTGTGCTGGTGATAGCATATCACTATGTCGTGGCTGACCCAAAGTATGAAGGAAACTAG

Protein sequence

>Msa1123500

LDSCASYLHQSFVRLYRTRPKKHDHNIRRRRNTHFHRVFKMIDDNDLGFFANFLGVFIFVLVIAYHYVVADPKYEGN*