AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa0521050


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0521050 MtrunA17_Chr3g0137181 100.000 51 0 0 1 51 1 51 2.66e-32 105
Msa0521050 MtrunA17_Chr1g0149761 100.000 51 0 0 1 51 1 51 2.66e-32 105
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0521050 AT4G31985.1 90.196 51 5 0 1 51 1 51 1.88e-29 98.2
Msa0521050 AT3G02190.1 88.235 51 6 0 1 51 1 51 4.41e-28 94.7
Msa0521050 AT2G25210.1 90.909 44 4 0 8 51 1 44 6.60e-24 84.0

Find 39 sgRNAs with CRISPR-Local

Find 132 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
AACTTATTGTAGTTAATTAA+AGG 0.251556 3_4:-73680026 Msa0521050:three_prime_UTR
ATAGGCTATTCATTGTGATA+TGG 0.254961 3_4:-73679907 Msa0521050:three_prime_UTR
AAGCAGCCGTCGAGCATTTC+CGG 0.266583 3_4:-73680548 Msa0521050:five_prime_UTR
ATTTAAGACAATCGATGTTT+TGG 0.282559 3_4:-73680105 Msa0521050:three_prime_UTR
TTAGATTAGGGCTTCGTGTT+AGG 0.305458 3_4:-73680580 Msa0521050:five_prime_UTR
AAGATTCTGAAATTAGATTA+GGG 0.315313 3_4:-73680592 Msa0521050:five_prime_UTR
AAAGATTCTGAAATTAGATT+AGG 0.315654 3_4:-73680593 Msa0521050:five_prime_UTR
ATTCGTAATCAAGAAGAAAT+TGG 0.321220 3_4:-73680406 Msa0521050:CDS
GGGATTTGAGTTCATTCTAT+AGG 0.365841 3_4:-73679925 Msa0521050:three_prime_UTR
AATGGCATACATCACCAATA+AGG 0.381130 3_4:+73679791 None:intergenic
CCGGAGGCAAGGATCGAAAA+TGG 0.389081 3_4:-73680529 Msa0521050:intron
GGCATTGTAATGTTATACTT+TGG 0.392128 3_4:-73680084 Msa0521050:three_prime_UTR
TTATTTCATCAAGGGAAATA+CGG 0.394107 3_4:-73679880 Msa0521050:three_prime_UTR
TAGGCTATTCATTGTGATAT+GGG 0.396854 3_4:-73679906 Msa0521050:three_prime_UTR
TCAAGCGAGTGCAATCAAAA+TGG 0.413755 3_4:+73679773 None:intergenic
AGAATAGACCCATCCCTTAC+TGG 0.418157 3_4:-73680368 Msa0521050:CDS
TAGATTAGGGCTTCGTGTTA+GGG 0.422723 3_4:-73680579 Msa0521050:five_prime_UTR
TCCCTTACTGGATCCGTATG+AGG 0.452674 3_4:-73680356 Msa0521050:CDS
CACTGGCGCCGTACCAAGCT+CGG 0.478359 3_4:-73680199 Msa0521050:CDS
AACCCTAATAGTTCAAGAAA+AGG 0.479806 3_4:+73680145 None:intergenic
TATTTCATCAAGGGAAATAC+GGG 0.488022 3_4:-73679879 Msa0521050:three_prime_UTR
ACAATGCTAAGCGCCGTCAC+TGG 0.497185 3_4:-73680216 Msa0521050:CDS
CTCAAAATCCGAGCTTGGTA+CGG 0.513650 3_4:+73680191 None:intergenic
GCTTGGTACGGCGCCAGTGA+CGG 0.518206 3_4:+73680203 None:intergenic
TGAGGACTGACAACACCATC+AGG 0.522876 3_4:-73680338 Msa0521050:intron
GTCCTCATACGGATCCAGTA+AGG 0.528387 3_4:+73680354 None:intergenic
GGACAAAACGCGGCAAAGCA+AGG 0.533748 3_4:+73680166 None:intergenic
GTCGAGCATTTCCGGAGGCA+AGG 0.547294 3_4:-73680540 Msa0521050:five_prime_UTR
TGGTGTTGTCAGTCCTCATA+CGG 0.570137 3_4:+73680343 None:intergenic
AGGACCTCAAAATCCGAGCT+TGG 0.583429 3_4:+73680186 None:intergenic
ATACGGATCCAGTAAGGGAT+GGG 0.586639 3_4:+73680360 None:intergenic
ACAATTTCCTAGTATACAGT+AGG 0.587677 3_4:+73679847 None:intergenic
CATACGGATCCAGTAAGGGA+TGG 0.594687 3_4:+73680359 None:intergenic
AGAAATTGGCGAAGAAGATG+AGG 0.597939 3_4:-73680392 Msa0521050:CDS
ATTACGAATGTCTTGTGTGA+CGG 0.598814 3_4:+73680420 None:intergenic
TCCTCATACGGATCCAGTAA+GGG 0.602358 3_4:+73680355 None:intergenic
TTGCCTCCGGAAATGCTCGA+CGG 0.662148 3_4:+73680542 None:intergenic
TTCAAGAAAAGGACAAAACG+CGG 0.690502 3_4:+73680156 None:intergenic
CAGCCGTCGAGCATTTCCGG+AGG 0.692580 3_4:-73680545 Msa0521050:five_prime_UTR

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! AAATCTGAATTAATTAAAAA+AGG + chr3_4:73680343-73680362 None:intergenic 10.0%
!! AAATCTGAATTAATTAAAAA+AGG + chr3_4:73680343-73680362 None:intergenic 10.0%
!!! TTTTATTTTTTTCCTCTTTT+AGG - chr3_4:73680097-73680116 Msa0521050:three_prime_UTR 15.0%
!! AACTTATTGTAGTTAATTAA+AGG - chr3_4:73680311-73680330 Msa0521050:intron 15.0%
!!! TAGGTTTTATATATTTTTGT+TGG - chr3_4:73680521-73680540 Msa0521050:intron 15.0%
!!! TTTTATTTTTTTCCTCTTTT+AGG - chr3_4:73680097-73680116 Msa0521050:three_prime_UTR 15.0%
!! AACTTATTGTAGTTAATTAA+AGG - chr3_4:73680311-73680330 Msa0521050:intron 15.0%
!!! TAGGTTTTATATATTTTTGT+TGG - chr3_4:73680521-73680540 Msa0521050:intron 15.0%
!!! AAAGATTCTGAAATTAGATT+AGG - chr3_4:73679744-73679763 Msa0521050:three_prime_UTR 20.0%
!!! AAGATTCTGAAATTAGATTA+GGG - chr3_4:73679745-73679764 Msa0521050:three_prime_UTR 20.0%
!!! TATATAAGTAAGAACTTTTG+CGG - chr3_4:73680279-73680298 Msa0521050:intron 20.0%
!!! TTATACATATTTAGTTTTGC+TGG - chr3_4:73680389-73680408 Msa0521050:CDS 20.0%
!!! TATACATATTTAGTTTTGCT+GGG - chr3_4:73680390-73680409 Msa0521050:CDS 20.0%
!! CAAAAATATATAAAACCTAG+AGG + chr3_4:73680520-73680539 None:intergenic 20.0%
!!! AAAGATTCTGAAATTAGATT+AGG - chr3_4:73679744-73679763 Msa0521050:three_prime_UTR 20.0%
!!! AAGATTCTGAAATTAGATTA+GGG - chr3_4:73679745-73679764 Msa0521050:three_prime_UTR 20.0%
!!! TATATAAGTAAGAACTTTTG+CGG - chr3_4:73680279-73680298 Msa0521050:intron 20.0%
!!! TTATACATATTTAGTTTTGC+TGG - chr3_4:73680389-73680408 Msa0521050:CDS 20.0%
!!! TATACATATTTAGTTTTGCT+GGG - chr3_4:73680390-73680409 Msa0521050:CDS 20.0%
!! CAAAAATATATAAAACCTAG+AGG + chr3_4:73680520-73680539 None:intergenic 20.0%
! ATTCGTAATCAAGAAGAAAT+TGG - chr3_4:73679931-73679950 Msa0521050:three_prime_UTR 25.0%
!!! ATTTAAGACAATCGATGTTT+TGG - chr3_4:73680232-73680251 Msa0521050:intron 25.0%
!!! ATACATATTTAGTTTTGCTG+GGG - chr3_4:73680391-73680410 Msa0521050:CDS 25.0%
!!! TATGGGATTTTATTTCATCA+AGG - chr3_4:73680448-73680467 Msa0521050:intron 25.0%
!!! ATGGGATTTTATTTCATCAA+GGG - chr3_4:73680449-73680468 Msa0521050:intron 25.0%
! TTATTTCATCAAGGGAAATA+CGG - chr3_4:73680457-73680476 Msa0521050:intron 25.0%
!!! TATTTTTGTTGGATCCTTAT+TGG - chr3_4:73680532-73680551 Msa0521050:exon 25.0%
! ATTCGTAATCAAGAAGAAAT+TGG - chr3_4:73679931-73679950 Msa0521050:three_prime_UTR 25.0%
!!! ATTTAAGACAATCGATGTTT+TGG - chr3_4:73680232-73680251 Msa0521050:intron 25.0%
!!! ATACATATTTAGTTTTGCTG+GGG - chr3_4:73680391-73680410 Msa0521050:CDS 25.0%
!!! TATGGGATTTTATTTCATCA+AGG - chr3_4:73680448-73680467 Msa0521050:intron 25.0%
!!! ATGGGATTTTATTTCATCAA+GGG - chr3_4:73680449-73680468 Msa0521050:intron 25.0%
! TTATTTCATCAAGGGAAATA+CGG - chr3_4:73680457-73680476 Msa0521050:intron 25.0%
!!! TATTTTTGTTGGATCCTTAT+TGG - chr3_4:73680532-73680551 Msa0521050:exon 25.0%
! TGTCCTTTTCTTGAACTATT+AGG - chr3_4:73680189-73680208 Msa0521050:exon 30.0%
! GTCCTTTTCTTGAACTATTA+GGG - chr3_4:73680190-73680209 Msa0521050:exon 30.0%
AACCCTAATAGTTCAAGAAA+AGG + chr3_4:73680195-73680214 None:intergenic 30.0%
GGCATTGTAATGTTATACTT+TGG - chr3_4:73680253-73680272 Msa0521050:intron 30.0%
!!! TACATATTTAGTTTTGCTGG+GGG - chr3_4:73680392-73680411 Msa0521050:CDS 30.0%
ATAGGCTATTCATTGTGATA+TGG - chr3_4:73680430-73680449 Msa0521050:intron 30.0%
TAGGCTATTCATTGTGATAT+GGG - chr3_4:73680431-73680450 Msa0521050:intron 30.0%
TATTTCATCAAGGGAAATAC+GGG - chr3_4:73680458-73680477 Msa0521050:intron 30.0%
! TTTTGTTCCTACTGTATACT+AGG - chr3_4:73680483-73680502 Msa0521050:intron 30.0%
ACAATTTCCTAGTATACAGT+AGG + chr3_4:73680493-73680512 None:intergenic 30.0%
!! TAGGAAATTGTTTTTCCTCT+AGG - chr3_4:73680502-73680521 Msa0521050:intron 30.0%
! TGTCCTTTTCTTGAACTATT+AGG - chr3_4:73680189-73680208 Msa0521050:exon 30.0%
! GTCCTTTTCTTGAACTATTA+GGG - chr3_4:73680190-73680209 Msa0521050:exon 30.0%
AACCCTAATAGTTCAAGAAA+AGG + chr3_4:73680195-73680214 None:intergenic 30.0%
GGCATTGTAATGTTATACTT+TGG - chr3_4:73680253-73680272 Msa0521050:intron 30.0%
!!! TACATATTTAGTTTTGCTGG+GGG - chr3_4:73680392-73680411 Msa0521050:CDS 30.0%
ATAGGCTATTCATTGTGATA+TGG - chr3_4:73680430-73680449 Msa0521050:intron 30.0%
TAGGCTATTCATTGTGATAT+GGG - chr3_4:73680431-73680450 Msa0521050:intron 30.0%
TATTTCATCAAGGGAAATAC+GGG - chr3_4:73680458-73680477 Msa0521050:intron 30.0%
! TTTTGTTCCTACTGTATACT+AGG - chr3_4:73680483-73680502 Msa0521050:intron 30.0%
ACAATTTCCTAGTATACAGT+AGG + chr3_4:73680493-73680512 None:intergenic 30.0%
!! TAGGAAATTGTTTTTCCTCT+AGG - chr3_4:73680502-73680521 Msa0521050:intron 30.0%
ATTACGAATGTCTTGTGTGA+CGG + chr3_4:73679920-73679939 None:intergenic 35.0%
CTTAGCATTGTACCTAAAAG+AGG + chr3_4:73680112-73680131 None:intergenic 35.0%
TTCAAGAAAAGGACAAAACG+CGG + chr3_4:73680184-73680203 None:intergenic 35.0%
! GGGATTTGAGTTCATTCTAT+AGG - chr3_4:73680412-73680431 Msa0521050:CDS 35.0%
AATGGCATACATCACCAATA+AGG + chr3_4:73680549-73680568 None:intergenic 35.0%
ATTACGAATGTCTTGTGTGA+CGG + chr3_4:73679920-73679939 None:intergenic 35.0%
CTTAGCATTGTACCTAAAAG+AGG + chr3_4:73680112-73680131 None:intergenic 35.0%
TTCAAGAAAAGGACAAAACG+CGG + chr3_4:73680184-73680203 None:intergenic 35.0%
! GGGATTTGAGTTCATTCTAT+AGG - chr3_4:73680412-73680431 Msa0521050:CDS 35.0%
AATGGCATACATCACCAATA+AGG + chr3_4:73680549-73680568 None:intergenic 35.0%
TTAGATTAGGGCTTCGTGTT+AGG - chr3_4:73679757-73679776 Msa0521050:three_prime_UTR 40.0%
TAGATTAGGGCTTCGTGTTA+GGG - chr3_4:73679758-73679777 Msa0521050:three_prime_UTR 40.0%
!! GATTGAAGAGACGCTGATTT+GGG + chr3_4:73679838-73679857 None:intergenic 40.0%
AGCGTCTCTTCAATCGTATT+AGG - chr3_4:73679843-73679862 Msa0521050:three_prime_UTR 40.0%
GCGTCTCTTCAATCGTATTA+GGG - chr3_4:73679844-73679863 Msa0521050:three_prime_UTR 40.0%
AGAAATTGGCGAAGAAGATG+AGG - chr3_4:73679945-73679964 Msa0521050:three_prime_UTR 40.0%
GAAAGGGAAAGAATACCTGA+TGG + chr3_4:73680017-73680036 None:intergenic 40.0%
AACGCGAAGAAAGAGTGAAA+GGG + chr3_4:73680033-73680052 None:intergenic 40.0%
AAACGCGAAGAAAGAGTGAA+AGG + chr3_4:73680034-73680053 None:intergenic 40.0%
! AAGAACTTTTGCGGTGTTTG+AGG - chr3_4:73680288-73680307 Msa0521050:intron 40.0%
TCAAGCGAGTGCAATCAAAA+TGG + chr3_4:73680567-73680586 None:intergenic 40.0%
TTAGATTAGGGCTTCGTGTT+AGG - chr3_4:73679757-73679776 Msa0521050:three_prime_UTR 40.0%
TAGATTAGGGCTTCGTGTTA+GGG - chr3_4:73679758-73679777 Msa0521050:three_prime_UTR 40.0%
!! GATTGAAGAGACGCTGATTT+GGG + chr3_4:73679838-73679857 None:intergenic 40.0%
AGCGTCTCTTCAATCGTATT+AGG - chr3_4:73679843-73679862 Msa0521050:three_prime_UTR 40.0%
GCGTCTCTTCAATCGTATTA+GGG - chr3_4:73679844-73679863 Msa0521050:three_prime_UTR 40.0%
AGAAATTGGCGAAGAAGATG+AGG - chr3_4:73679945-73679964 Msa0521050:three_prime_UTR 40.0%
GAAAGGGAAAGAATACCTGA+TGG + chr3_4:73680017-73680036 None:intergenic 40.0%
AACGCGAAGAAAGAGTGAAA+GGG + chr3_4:73680033-73680052 None:intergenic 40.0%
AAACGCGAAGAAAGAGTGAA+AGG + chr3_4:73680034-73680053 None:intergenic 40.0%
! AAGAACTTTTGCGGTGTTTG+AGG - chr3_4:73680288-73680307 Msa0521050:intron 40.0%
TCAAGCGAGTGCAATCAAAA+TGG + chr3_4:73680567-73680586 None:intergenic 40.0%
! CGATTGAAGAGACGCTGATT+TGG + chr3_4:73679839-73679858 None:intergenic 45.0%
AGAATAGACCCATCCCTTAC+TGG - chr3_4:73679969-73679988 Msa0521050:three_prime_UTR 45.0%
ATACGGATCCAGTAAGGGAT+GGG + chr3_4:73679980-73679999 None:intergenic 45.0%
TCCTCATACGGATCCAGTAA+GGG + chr3_4:73679985-73680004 None:intergenic 45.0%
!! TGGTGTTGTCAGTCCTCATA+CGG + chr3_4:73679997-73680016 None:intergenic 45.0%
CTCAAAATCCGAGCTTGGTA+CGG + chr3_4:73680149-73680168 None:intergenic 45.0%
! CGATTGAAGAGACGCTGATT+TGG + chr3_4:73679839-73679858 None:intergenic 45.0%
AGAATAGACCCATCCCTTAC+TGG - chr3_4:73679969-73679988 Msa0521050:three_prime_UTR 45.0%
ATACGGATCCAGTAAGGGAT+GGG + chr3_4:73679980-73679999 None:intergenic 45.0%
TCCTCATACGGATCCAGTAA+GGG + chr3_4:73679985-73680004 None:intergenic 45.0%
!! TGGTGTTGTCAGTCCTCATA+CGG + chr3_4:73679997-73680016 None:intergenic 45.0%
CTCAAAATCCGAGCTTGGTA+CGG + chr3_4:73680149-73680168 None:intergenic 45.0%
! CCATTTTCGATCCTTGCCTC+CGG + chr3_4:73679811-73679830 None:intergenic 50.0%
CATACGGATCCAGTAAGGGA+TGG + chr3_4:73679981-73680000 None:intergenic 50.0%
TCCCTTACTGGATCCGTATG+AGG - chr3_4:73679981-73680000 Msa0521050:three_prime_UTR 50.0%
GTCCTCATACGGATCCAGTA+AGG + chr3_4:73679986-73680005 None:intergenic 50.0%
TGAGGACTGACAACACCATC+AGG - chr3_4:73679999-73680018 Msa0521050:three_prime_UTR 50.0%
! CGTACCAAGCTCGGATTTTG+AGG - chr3_4:73680147-73680166 Msa0521050:three_prime_UTR 50.0%
AGGACCTCAAAATCCGAGCT+TGG + chr3_4:73680154-73680173 None:intergenic 50.0%
! CCATTTTCGATCCTTGCCTC+CGG + chr3_4:73679811-73679830 None:intergenic 50.0%
CATACGGATCCAGTAAGGGA+TGG + chr3_4:73679981-73680000 None:intergenic 50.0%
TCCCTTACTGGATCCGTATG+AGG - chr3_4:73679981-73680000 Msa0521050:three_prime_UTR 50.0%
GTCCTCATACGGATCCAGTA+AGG + chr3_4:73679986-73680005 None:intergenic 50.0%
TGAGGACTGACAACACCATC+AGG - chr3_4:73679999-73680018 Msa0521050:three_prime_UTR 50.0%
! CGTACCAAGCTCGGATTTTG+AGG - chr3_4:73680147-73680166 Msa0521050:three_prime_UTR 50.0%
AGGACCTCAAAATCCGAGCT+TGG + chr3_4:73680154-73680173 None:intergenic 50.0%
AAGCAGCCGTCGAGCATTTC+CGG - chr3_4:73679789-73679808 Msa0521050:three_prime_UTR 55.0%
TTGCCTCCGGAAATGCTCGA+CGG + chr3_4:73679798-73679817 None:intergenic 55.0%
CCGGAGGCAAGGATCGAAAA+TGG - chr3_4:73679808-73679827 Msa0521050:three_prime_UTR 55.0%
! ACAATGCTAAGCGCCGTCAC+TGG - chr3_4:73680121-73680140 Msa0521050:three_prime_UTR 55.0%
GGACAAAACGCGGCAAAGCA+AGG + chr3_4:73680174-73680193 None:intergenic 55.0%
AAGCAGCCGTCGAGCATTTC+CGG - chr3_4:73679789-73679808 Msa0521050:three_prime_UTR 55.0%
TTGCCTCCGGAAATGCTCGA+CGG + chr3_4:73679798-73679817 None:intergenic 55.0%
CCGGAGGCAAGGATCGAAAA+TGG - chr3_4:73679808-73679827 Msa0521050:three_prime_UTR 55.0%
! ACAATGCTAAGCGCCGTCAC+TGG - chr3_4:73680121-73680140 Msa0521050:three_prime_UTR 55.0%
GGACAAAACGCGGCAAAGCA+AGG + chr3_4:73680174-73680193 None:intergenic 55.0%
GTCGAGCATTTCCGGAGGCA+AGG - chr3_4:73679797-73679816 Msa0521050:three_prime_UTR 60.0%
GTCGAGCATTTCCGGAGGCA+AGG - chr3_4:73679797-73679816 Msa0521050:three_prime_UTR 60.0%
CAGCCGTCGAGCATTTCCGG+AGG - chr3_4:73679792-73679811 Msa0521050:three_prime_UTR 65.0%
GCTTGGTACGGCGCCAGTGA+CGG + chr3_4:73680137-73680156 None:intergenic 65.0%
CACTGGCGCCGTACCAAGCT+CGG - chr3_4:73680138-73680157 Msa0521050:three_prime_UTR 65.0%
CAGCCGTCGAGCATTTCCGG+AGG - chr3_4:73679792-73679811 Msa0521050:three_prime_UTR 65.0%
GCTTGGTACGGCGCCAGTGA+CGG + chr3_4:73680137-73680156 None:intergenic 65.0%
CACTGGCGCCGTACCAAGCT+CGG - chr3_4:73680138-73680157 Msa0521050:three_prime_UTR 65.0%
Chromosome Type Strat End Strand Name
chr3_4 gene 73679744 73680615 73679744 ID=Msa0521050;Name=Msa0521050
chr3_4 mRNA 73679744 73680615 73679744 ID=Msa0521050-mRNA-1;Parent=Msa0521050;Name=Msa0521050-mRNA-1;_AED=0.01;_eAED=0.01;_QI=83|1|1|1|1|1|3|448|51
chr3_4 exon 73679744 73680240 73679744 ID=Msa0521050-mRNA-1:exon:20721;Parent=Msa0521050-mRNA-1
chr3_4 exon 73680339 73680442 73680339 ID=Msa0521050-mRNA-1:exon:20720;Parent=Msa0521050-mRNA-1
chr3_4 exon 73680530 73680615 73680530 ID=Msa0521050-mRNA-1:exon:20719;Parent=Msa0521050-mRNA-1
chr3_4 five_prime_UTR 73680533 73680615 73680533 ID=Msa0521050-mRNA-1:five_prime_utr;Parent=Msa0521050-mRNA-1
chr3_4 CDS 73680530 73680532 73680530 ID=Msa0521050-mRNA-1:cds;Parent=Msa0521050-mRNA-1
chr3_4 CDS 73680339 73680442 73680339 ID=Msa0521050-mRNA-1:cds;Parent=Msa0521050-mRNA-1
chr3_4 CDS 73680192 73680240 73680192 ID=Msa0521050-mRNA-1:cds;Parent=Msa0521050-mRNA-1
chr3_4 three_prime_UTR 73679744 73680191 73679744 ID=Msa0521050-mRNA-1:three_prime_utr;Parent=Msa0521050-mRNA-1
Gene Sequence

>Msa0521050

ATGCCGTCACACAAGACATTCGTAATCAAGAAGAAATTGGCGAAGAAGATGAGGCAGAATAGACCCATCCCTTACTGGATCCGTATGAGGACTGACAACACCATCAGGTACAATGCTAAGCGCCGTCACTGGCGCCGTACCAAGCTCGGATTTTGA

Protein sequence

>Msa0521050

MPSHKTFVIKKKLAKKMRQNRPIPYWIRMRTDNTIRYNAKRRHWRRTKLGF*