AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa1169770


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa1169770 AT3G12587.1 81.081 37 7 0 41 77 1 37 1.20e-16 66.6
Msa1169770 AT3G09455.2 80.000 35 7 0 41 75 1 35 9.44e-16 64.3
Msa1169770 AT3G09455.1 80.000 35 7 0 41 75 1 35 9.44e-16 64.3
Msa1169770 AT5G02502.1 77.143 35 8 0 41 75 1 35 2.17e-15 63.5

Find 20 sgRNAs with CRISPR-Local

Find 125 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
TATAATTATGAACCTCGTTT+TGG 0.249507 7_4:+62848481 Msa1169770:three_prime_UTR
TCTAGTTTCCTTCATACTTT+GGG 0.296845 7_4:-62848384 None:intergenic
ATGTTGTGGTCGTGCTTCTT+AGG 0.309733 7_4:-62847288 None:intergenic
TTCTAGTTTCCTTCATACTT+TGG 0.311802 7_4:-62848385 None:intergenic
GATGAAGACGCCGAGAAAAT+TGG 0.325577 7_4:-62848323 None:intergenic
CCTTGAAAACGCGGTGGAAA+TGG 0.326898 7_4:-62847329 None:intergenic
CGGCGTCTTCATCTTTGTGC+TGG 0.380443 7_4:+62848333 Msa1169770:CDS
GATGATTGATGACAATGATC+TGG 0.432151 7_4:+62848291 Msa1169770:intron
CTTGAAAACGCGGTGGAAAT+GGG 0.434606 7_4:-62847328 None:intergenic
GGAAATGGGTGTTTCTTCTC+CGG 0.476260 7_4:-62847314 None:intergenic
TGCTTATGTGTGCTTAAGAC+CGG 0.487149 7_4:+62848579 Msa1169770:three_prime_UTR
TTCTTCTCCGGCGAATGTTG+TGG 0.493038 7_4:-62847302 None:intergenic
GTGGCTGACCCAAAGTATGA+AGG 0.567065 7_4:+62848376 Msa1169770:CDS
AGACTGAGATGCTTTACAGA+TGG 0.571757 7_4:+62848446 Msa1169770:three_prime_UTR
ATGATTGATGACAATGATCT+GGG 0.577930 7_4:+62848292 Msa1169770:CDS
AGCACGACCACAACATTCGC+CGG 0.611769 7_4:+62847295 Msa1169770:CDS
ATGATACCTTGAAAACGCGG+TGG 0.684438 7_4:-62847335 None:intergenic
AAATTAATATTCCCAAAACG+AGG 0.696429 7_4:-62848493 None:intergenic
GCGATGATACCTTGAAAACG+CGG 0.740904 7_4:-62847338 None:intergenic
GATAGCATATCACTATGTCG+TGG 0.751623 7_4:+62848357 Msa1169770:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! ATAAAATAAAATAATGATGA+TGG - chr7_4:62847410-62847429 None:intergenic 10.0%
!! ATAACAAAACAAAAAAAAAA+TGG - chr7_4:62848560-62848579 None:intergenic 10.0%
!! ATAAAATAAAATAATGATGA+TGG - chr7_4:62847410-62847429 None:intergenic 10.0%
!! ATAACAAAACAAAAAAAAAA+TGG - chr7_4:62848560-62848579 None:intergenic 10.0%
!! CCTATATTACTATAAAAATT+GGG + chr7_4:62848046-62848065 Msa1169770:intron 15.0%
!! CCTATATTACTATAAAAATT+GGG + chr7_4:62848046-62848065 Msa1169770:intron 15.0%
!!! CCTAAATTTTTCAGATAATT+CGG + chr7_4:62847578-62847597 Msa1169770:intron 20.0%
!!! GTTTTGATTTTAAGTTGTAA+AGG + chr7_4:62847645-62847664 Msa1169770:intron 20.0%
!!! AAAGGATTTAAGATTTGATT+AGG + chr7_4:62847663-62847682 Msa1169770:intron 20.0%
!! GAGAAAATATATGTTACAAT+TGG + chr7_4:62847713-62847732 Msa1169770:intron 20.0%
!! TAAAAATTGTTGACAAATGT+TGG + chr7_4:62847746-62847765 Msa1169770:intron 20.0%
!!! ATTTAAGTTGTTTTTGAAAC+AGG + chr7_4:62848022-62848041 Msa1169770:intron 20.0%
!!! TTTAAGTTGTTTTTGAAACA+GGG + chr7_4:62848023-62848042 Msa1169770:intron 20.0%
!! GCCTATATTACTATAAAAAT+TGG + chr7_4:62848045-62848064 Msa1169770:intron 20.0%
!!! CCCAATTTTTATAGTAATAT+AGG - chr7_4:62848049-62848068 None:intergenic 20.0%
!!! GAAAATCAATCTACTTTTTT+AGG - chr7_4:62848200-62848219 None:intergenic 20.0%
!!! CCTAAATTTTTCAGATAATT+CGG + chr7_4:62847578-62847597 Msa1169770:intron 20.0%
!!! GTTTTGATTTTAAGTTGTAA+AGG + chr7_4:62847645-62847664 Msa1169770:intron 20.0%
!!! AAAGGATTTAAGATTTGATT+AGG + chr7_4:62847663-62847682 Msa1169770:intron 20.0%
!! GAGAAAATATATGTTACAAT+TGG + chr7_4:62847713-62847732 Msa1169770:intron 20.0%
!! TAAAAATTGTTGACAAATGT+TGG + chr7_4:62847746-62847765 Msa1169770:intron 20.0%
!!! ATTTAAGTTGTTTTTGAAAC+AGG + chr7_4:62848022-62848041 Msa1169770:intron 20.0%
!!! TTTAAGTTGTTTTTGAAACA+GGG + chr7_4:62848023-62848042 Msa1169770:intron 20.0%
!! GCCTATATTACTATAAAAAT+TGG + chr7_4:62848045-62848064 Msa1169770:intron 20.0%
!!! CCCAATTTTTATAGTAATAT+AGG - chr7_4:62848049-62848068 None:intergenic 20.0%
!!! GAAAATCAATCTACTTTTTT+AGG - chr7_4:62848200-62848219 None:intergenic 20.0%
! CCGAATTATCTGAAAAATTT+AGG - chr7_4:62847581-62847600 None:intergenic 25.0%
!! TTTGTCAACAATTTTTAACC+AGG - chr7_4:62847743-62847762 None:intergenic 25.0%
! TTACTATAAAAATTGGGATC+TGG + chr7_4:62848052-62848071 Msa1169770:intron 25.0%
! TACTATAAAAATTGGGATCT+GGG + chr7_4:62848053-62848072 Msa1169770:intron 25.0%
!! TATAATTATGAACCTCGTTT+TGG + chr7_4:62848481-62848500 Msa1169770:three_prime_UTR 25.0%
!! ATAATTATGAACCTCGTTTT+GGG + chr7_4:62848482-62848501 Msa1169770:three_prime_UTR 25.0%
! AAATTAATATTCCCAAAACG+AGG - chr7_4:62848496-62848515 None:intergenic 25.0%
!!! TGGGAATATTAATTTTAGCT+TGG + chr7_4:62848501-62848520 Msa1169770:three_prime_UTR 25.0%
! CCGAATTATCTGAAAAATTT+AGG - chr7_4:62847581-62847600 None:intergenic 25.0%
!! TTTGTCAACAATTTTTAACC+AGG - chr7_4:62847743-62847762 None:intergenic 25.0%
! TTACTATAAAAATTGGGATC+TGG + chr7_4:62848052-62848071 Msa1169770:intron 25.0%
! TACTATAAAAATTGGGATCT+GGG + chr7_4:62848053-62848072 Msa1169770:intron 25.0%
!! TATAATTATGAACCTCGTTT+TGG + chr7_4:62848481-62848500 Msa1169770:three_prime_UTR 25.0%
!! ATAATTATGAACCTCGTTTT+GGG + chr7_4:62848482-62848501 Msa1169770:three_prime_UTR 25.0%
! AAATTAATATTCCCAAAACG+AGG - chr7_4:62848496-62848515 None:intergenic 25.0%
!!! TGGGAATATTAATTTTAGCT+TGG + chr7_4:62848501-62848520 Msa1169770:three_prime_UTR 25.0%
!! ATTTGCATCAGAGTTTTGTA+AGG + chr7_4:62847250-62847269 Msa1169770:CDS 30.0%
! TGATTTACGAGTTTTCAGAT+CGG + chr7_4:62847515-62847534 Msa1169770:intron 30.0%
TGATAAGAAGTTTAGCTAGT+TGG + chr7_4:62848103-62848122 Msa1169770:intron 30.0%
ATTCAAATGTGCAACAAGAA+GGG - chr7_4:62848137-62848156 None:intergenic 30.0%
AATTCAAATGTGCAACAAGA+AGG - chr7_4:62848138-62848157 None:intergenic 30.0%
TTGTTGCACATTTGAATTTG+TGG + chr7_4:62848140-62848159 Msa1169770:intron 30.0%
! ATTTGAATTTGTGGTCTTGA+TGG + chr7_4:62848149-62848168 Msa1169770:intron 30.0%
! ATTTTGTGATATCACAAACC+AGG + chr7_4:62848233-62848252 Msa1169770:intron 30.0%
! ATGATTGATGACAATGATCT+GGG + chr7_4:62848292-62848311 Msa1169770:CDS 30.0%
! TCTAGTTTCCTTCATACTTT+GGG - chr7_4:62848387-62848406 None:intergenic 30.0%
!! TTCTAGTTTCCTTCATACTT+TGG - chr7_4:62848388-62848407 None:intergenic 30.0%
!! ATTTGCATCAGAGTTTTGTA+AGG + chr7_4:62847250-62847269 Msa1169770:CDS 30.0%
! TGATTTACGAGTTTTCAGAT+CGG + chr7_4:62847515-62847534 Msa1169770:intron 30.0%
TGATAAGAAGTTTAGCTAGT+TGG + chr7_4:62848103-62848122 Msa1169770:intron 30.0%
ATTCAAATGTGCAACAAGAA+GGG - chr7_4:62848137-62848156 None:intergenic 30.0%
AATTCAAATGTGCAACAAGA+AGG - chr7_4:62848138-62848157 None:intergenic 30.0%
TTGTTGCACATTTGAATTTG+TGG + chr7_4:62848140-62848159 Msa1169770:intron 30.0%
! ATTTGAATTTGTGGTCTTGA+TGG + chr7_4:62848149-62848168 Msa1169770:intron 30.0%
! ATTTTGTGATATCACAAACC+AGG + chr7_4:62848233-62848252 Msa1169770:intron 30.0%
! ATGATTGATGACAATGATCT+GGG + chr7_4:62848292-62848311 Msa1169770:CDS 30.0%
! TCTAGTTTCCTTCATACTTT+GGG - chr7_4:62848387-62848406 None:intergenic 30.0%
!! TTCTAGTTTCCTTCATACTT+TGG - chr7_4:62848388-62848407 None:intergenic 30.0%
AGAAACGAGAATTGAGAAGA+AGG - chr7_4:62847363-62847382 None:intergenic 35.0%
! GATTTACGAGTTTTCAGATC+GGG + chr7_4:62847516-62847535 Msa1169770:intron 35.0%
! ATTTACGAGTTTTCAGATCG+GGG + chr7_4:62847517-62847536 Msa1169770:intron 35.0%
TATGTTACAATTGGTAAGCC+TGG + chr7_4:62847722-62847741 Msa1169770:intron 35.0%
TCAGGCTCACAATATATCAA+AGG - chr7_4:62847821-62847840 None:intergenic 35.0%
TGATATATTGTGAGCCTGAT+TGG + chr7_4:62847822-62847841 Msa1169770:intron 35.0%
TCAAACACATCAATCCAATC+AGG - chr7_4:62847839-62847858 None:intergenic 35.0%
TAAGCACTTGTGAAACTGTT+TGG + chr7_4:62847873-62847892 Msa1169770:intron 35.0%
TGGTATGCTTGATTGTCATA+TGG + chr7_4:62848169-62848188 Msa1169770:intron 35.0%
AAAAATATTCGTGAGTGACC+TGG - chr7_4:62848254-62848273 None:intergenic 35.0%
! GATGATTGATGACAATGATC+TGG + chr7_4:62848291-62848310 Msa1169770:intron 35.0%
! GGTTTCTTTGCCAATTTTCT+CGG + chr7_4:62848313-62848332 Msa1169770:CDS 35.0%
AGAAACGAGAATTGAGAAGA+AGG - chr7_4:62847363-62847382 None:intergenic 35.0%
! GATTTACGAGTTTTCAGATC+GGG + chr7_4:62847516-62847535 Msa1169770:intron 35.0%
! ATTTACGAGTTTTCAGATCG+GGG + chr7_4:62847517-62847536 Msa1169770:intron 35.0%
TATGTTACAATTGGTAAGCC+TGG + chr7_4:62847722-62847741 Msa1169770:intron 35.0%
TCAGGCTCACAATATATCAA+AGG - chr7_4:62847821-62847840 None:intergenic 35.0%
TGATATATTGTGAGCCTGAT+TGG + chr7_4:62847822-62847841 Msa1169770:intron 35.0%
TCAAACACATCAATCCAATC+AGG - chr7_4:62847839-62847858 None:intergenic 35.0%
TAAGCACTTGTGAAACTGTT+TGG + chr7_4:62847873-62847892 Msa1169770:intron 35.0%
TGGTATGCTTGATTGTCATA+TGG + chr7_4:62848169-62848188 Msa1169770:intron 35.0%
AAAAATATTCGTGAGTGACC+TGG - chr7_4:62848254-62848273 None:intergenic 35.0%
! GATGATTGATGACAATGATC+TGG + chr7_4:62848291-62848310 Msa1169770:intron 35.0%
! GGTTTCTTTGCCAATTTTCT+CGG + chr7_4:62848313-62848332 Msa1169770:CDS 35.0%
ATGCCGACACTTATTCTTAC+AGG + chr7_4:62847469-62847488 Msa1169770:intron 40.0%
GCACCTGTAAGAATAAGTGT+CGG - chr7_4:62847475-62847494 None:intergenic 40.0%
CTGTAAATGCAATCCTGATG+TGG + chr7_4:62847777-62847796 Msa1169770:intron 40.0%
AAAATCTCTGCAACCACATC+AGG - chr7_4:62847793-62847812 None:intergenic 40.0%
GATAGCATATCACTATGTCG+TGG + chr7_4:62848357-62848376 Msa1169770:CDS 40.0%
AGACTGAGATGCTTTACAGA+TGG + chr7_4:62848446-62848465 Msa1169770:three_prime_UTR 40.0%
TAACTAGCACAAGAGTCTAG+CGG - chr7_4:62847231-62847250 None:intergenic 40.0%
ATGCCGACACTTATTCTTAC+AGG + chr7_4:62847469-62847488 Msa1169770:intron 40.0%
GCACCTGTAAGAATAAGTGT+CGG - chr7_4:62847475-62847494 None:intergenic 40.0%
CTGTAAATGCAATCCTGATG+TGG + chr7_4:62847777-62847796 Msa1169770:intron 40.0%
AAAATCTCTGCAACCACATC+AGG - chr7_4:62847793-62847812 None:intergenic 40.0%
GATAGCATATCACTATGTCG+TGG + chr7_4:62848357-62848376 Msa1169770:CDS 40.0%
AGACTGAGATGCTTTACAGA+TGG + chr7_4:62848446-62848465 Msa1169770:three_prime_UTR 40.0%
ATGTTGTGGTCGTGCTTCTT+AGG - chr7_4:62847291-62847310 None:intergenic 45.0%
GGAAATGGGTGTTTCTTCTC+CGG - chr7_4:62847317-62847336 None:intergenic 45.0%
CTTGAAAACGCGGTGGAAAT+GGG - chr7_4:62847331-62847350 None:intergenic 45.0%
ATGATACCTTGAAAACGCGG+TGG - chr7_4:62847338-62847357 None:intergenic 45.0%
GCGATGATACCTTGAAAACG+CGG - chr7_4:62847341-62847360 None:intergenic 45.0%
GATGAAGACGCCGAGAAAAT+TGG - chr7_4:62848326-62848345 None:intergenic 45.0%
ATGTTGTGGTCGTGCTTCTT+AGG - chr7_4:62847291-62847310 None:intergenic 45.0%
GGAAATGGGTGTTTCTTCTC+CGG - chr7_4:62847317-62847336 None:intergenic 45.0%
CTTGAAAACGCGGTGGAAAT+GGG - chr7_4:62847331-62847350 None:intergenic 45.0%
ATGATACCTTGAAAACGCGG+TGG - chr7_4:62847338-62847357 None:intergenic 45.0%
GCGATGATACCTTGAAAACG+CGG - chr7_4:62847341-62847360 None:intergenic 45.0%
GATGAAGACGCCGAGAAAAT+TGG - chr7_4:62848326-62848345 None:intergenic 45.0%
TTCTTCTCCGGCGAATGTTG+TGG - chr7_4:62847305-62847324 None:intergenic 50.0%
CCTTGAAAACGCGGTGGAAA+TGG - chr7_4:62847332-62847351 None:intergenic 50.0%
! CCATTTCCACCGCGTTTTCA+AGG + chr7_4:62847329-62847348 Msa1169770:CDS 50.0%
GTGGCTGACCCAAAGTATGA+AGG + chr7_4:62848376-62848395 Msa1169770:CDS 50.0%
TTCTTCTCCGGCGAATGTTG+TGG - chr7_4:62847305-62847324 None:intergenic 50.0%
CCTTGAAAACGCGGTGGAAA+TGG - chr7_4:62847332-62847351 None:intergenic 50.0%
! CCATTTCCACCGCGTTTTCA+AGG + chr7_4:62847329-62847348 Msa1169770:CDS 50.0%
GTGGCTGACCCAAAGTATGA+AGG + chr7_4:62848376-62848395 Msa1169770:CDS 50.0%
AGCACGACCACAACATTCGC+CGG + chr7_4:62847295-62847314 Msa1169770:CDS 55.0%
CGGCGTCTTCATCTTTGTGC+TGG + chr7_4:62848333-62848352 Msa1169770:CDS 55.0%
AGCACGACCACAACATTCGC+CGG + chr7_4:62847295-62847314 Msa1169770:CDS 55.0%
CGGCGTCTTCATCTTTGTGC+TGG + chr7_4:62848333-62848352 Msa1169770:CDS 55.0%
Chromosome Type Strat End Strand Name
chr7_4 gene 62847231 62848600 62847231 ID=Msa1169770;Name=Msa1169770
chr7_4 mRNA 62847231 62848600 62847231 ID=Msa1169770-mRNA-1;Parent=Msa1169770;Name=Msa1169770-mRNA-1;_AED=0.05;_eAED=0.05;_QI=0|1|1|1|1|1|2|195|77
chr7_4 exon 62847231 62847350 62847231 ID=Msa1169770-mRNA-1:exon:20012;Parent=Msa1169770-mRNA-1
chr7_4 exon 62848292 62848600 62848292 ID=Msa1169770-mRNA-1:exon:20013;Parent=Msa1169770-mRNA-1
chr7_4 CDS 62847231 62847350 62847231 ID=Msa1169770-mRNA-1:cds;Parent=Msa1169770-mRNA-1
chr7_4 CDS 62848292 62848405 62848292 ID=Msa1169770-mRNA-1:cds;Parent=Msa1169770-mRNA-1
chr7_4 three_prime_UTR 62848406 62848600 62848406 ID=Msa1169770-mRNA-1:three_prime_utr;Parent=Msa1169770-mRNA-1
Gene Sequence

>Msa1169770

CTAGACTCTTGTGCTAGTTATTTGCATCAGAGTTTTGTAAGGTTATACAGAACGAGACCTAAGAAGCACGACCACAACATTCGCCGGAGAAGAAACACCCATTTCCACCGCGTTTTCAAGATGATTGATGACAATGATCTGGGTTTCTTTGCCAATTTTCTCGGCGTCTTCATCTTTGTGCTGGTGATAGCATATCACTATGTCGTGGCTGACCCAAAGTATGAAGGAAACTAG

Protein sequence

>Msa1169770

LDSCASYLHQSFVRLYRTRPKKHDHNIRRRRNTHFHRVFKMIDDNDLGFFANFLGVFIFVLVIAYHYVVADPKYEGN*