AlfalfaGEDB | Msa1078270

M. sativa cultivar zhongmu-4 / Msa1078270

KEGG: -
GO: -
PFAM: PF10215.12

Nr:

Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa1078270	KAG6484884.1	94.595	37	2	0	1	37	80	116	1.37e-17	79.3

Swissprot:

Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa1078270	sp\|C7J4U3\|OST4A_ORYSJ	86.486	37	5	0	1	37	1	37	4.84e-17	68.6

Trembl:

Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa1078270	G7KZM4	100.000	37	0	0	1	37	35	71	1.40e-17	77.0

TFs/TRs:

Gene ID	Type	Classification

Protein Kinases:

Gene ID	Type	Classification

Network:

Co-expression Network

Gene1	Gene2	correlation coefficient	p_value	FDR
Msa0001450	Msa1078270	0.800566	1.357033e-48	-8.615850e-47
Msa0046740	Msa1078270	0.827274	1.712377e-54	-8.615850e-47
Msa0054230	Msa1078270	0.818993	1.466297e-52	-8.615850e-47
Msa0056430	Msa1078270	0.808268	3.366979e-50	-8.615850e-47
Msa0056470	Msa1078270	0.812248	4.665174e-51	-8.615850e-47
Msa0064350	Msa1078270	0.830226	3.307920e-55	-8.615850e-47
Msa0064380	Msa1078270	0.846700	1.841950e-59	-8.615850e-47
Msa0076040	Msa1078270	0.812964	3.252248e-51	-8.615850e-47
Msa0082280	Msa1078270	0.804430	2.168196e-49	-8.615850e-47
Msa0115990	Msa1078270	0.863141	3.076118e-64	-8.615850e-47
Msa0615990	Msa1078270	0.809660	1.695778e-50	-8.615850e-47
Msa0651050	Msa1078270	0.875920	2.106546e-68	-8.615850e-47
Msa0692840	Msa1078270	0.840463	8.563574e-58	-8.615850e-47
Msa0692940	Msa1078270	0.823919	1.068650e-53	-8.615850e-47
Msa0708000	Msa1078270	-0.804396	2.203868e-49	-8.615850e-47
Msa0784200	Msa1078270	0.853796	1.886025e-61	-8.615850e-47
Msa0818310	Msa1078270	0.843545	1.311761e-58	-8.615850e-47
Msa0123560	Msa1078270	0.804556	2.040977e-49	-8.615850e-47
Msa0149190	Msa1078270	0.812837	3.467388e-51	-8.615850e-47
Msa0149200	Msa1078270	0.847306	1.257096e-59	-8.615850e-47
Msa0199880	Msa1078270	0.802050	6.742009e-49	-8.615850e-47
Msa0211840	Msa1078270	0.807695	4.458412e-50	-8.615850e-47
Msa0369450	Msa1078270	0.814214	1.726820e-51	-8.615850e-47
Msa0380300	Msa1078270	0.807405	5.136463e-50	-8.615850e-47
Msa0385250	Msa1078270	0.803647	3.155077e-49	-8.615850e-47
Msa0432210	Msa1078270	0.823679	1.216752e-53	-8.615850e-47
Msa1078270	Msa1081990	0.830247	3.270770e-55	-8.615850e-47
Msa1078270	Msa1123500	0.979530	3.593354e-148	-8.615850e-47
Msa1078270	Msa1126510	0.824107	9.652471e-54	-8.615850e-47
Msa1078270	Msa1126770	0.808774	2.624870e-50	-8.615850e-47
Msa1078270	Msa1165940	0.812647	3.816196e-51	-8.615850e-47
Msa1078270	Msa1166860	0.811532	6.680174e-51	-8.615850e-47
Msa1078270	Msa1169770	0.998879	3.377387e-280	-8.615850e-47
Msa1078270	Msa1218600	0.817104	3.920132e-52	-8.615850e-47
Msa1078270	Msa1239910	0.814214	1.726859e-51	-8.615850e-47
Msa1078270	Msa1251160	0.802456	5.562035e-49	-8.615850e-47
Msa1078270	Msa1257510	0.813746	2.189536e-51	-8.615850e-47
Msa1078270	Msa1361100	0.800016	1.756436e-48	-8.615850e-47
Msa1078270	Msa1413260	0.815701	8.077803e-52	-8.615850e-47
Msa1078270	Msa1450280	0.816487	5.391779e-52	-8.615850e-47
Msa1078270	Msa1456050	0.850757	1.381401e-60	-8.615850e-47
Msa0315170	Msa1078270	0.837665	4.546395e-57	-8.615850e-47
Msa0521050	Msa1078270	0.892442	1.581453e-74	-8.615850e-47
Msa0552810	Msa1078270	0.825482	4.577064e-54	-8.615850e-47
Msa0566700	Msa1078270	0.841365	4.965528e-58	-8.615850e-47
Msa0580280	Msa1078270	0.801335	9.454254e-49	-8.615850e-47
Msa0581070	Msa1078270	0.812788	3.553879e-51	-8.615850e-47
Msa0858770	Msa1078270	0.855287	6.980250e-62	-8.615850e-47

PPI

Gene1	Gene2	Type

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score

Query id	Subject id	identity %	alignment length	mismatches	q. start	q. end	s. start	s. end	e-value	bit score
Msa1078270	AT3G12587.1	81.081	37	7	1	37	1	37	8.47e-17	65.1
Msa1078270	AT3G09455.2	80.000	35	7	1	35	1	35	1.01e-15	62.4
Msa1078270	AT3G09455.1	80.000	35	7	1	35	1	35	1.01e-15	62.4
Msa1078270	AT5G02502.1	77.143	35	8	1	35	1	35	2.12e-15	61.6

Find 20 sgRNAs with CRISPR-Local

Find 129 sgRNAs with CRISPR-GE

CRISPR-Local

CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
TATAATTATGAACCTCGTTT+TGG	0.249507	7_2:+77154478	Msa1078270:three_prime_UTR
TCTAGTTTCCTTCATACTTT+GGG	0.296845	7_2:-77154381	None:intergenic
ATGTTGTGGTCGTGCTTCTT+AGG	0.309733	7_2:-77153284	None:intergenic
TTCTAGTTTCCTTCATACTT+TGG	0.311802	7_2:-77154382	None:intergenic
GATGAAGACGCCGAGAAAAT+TGG	0.325577	7_2:-77154320	None:intergenic
CCTTGAAAACGCGGTGGAAA+TGG	0.326898	7_2:-77153325	None:intergenic
CGGCGTCTTCATCTTTGTGC+TGG	0.380443	7_2:+77154330	Msa1078270:CDS
GATGATTGATGACAATGATC+TGG	0.432151	7_2:+77154288	Msa1078270:intron
CTTGAAAACGCGGTGGAAAT+GGG	0.434606	7_2:-77153324	None:intergenic
TGCTTATGGGTGCTTAAGAC+CGG	0.459091	7_2:+77154575	Msa1078270:three_prime_UTR
GGAAATGGGTGTTTCTTCTC+CGG	0.476260	7_2:-77153310	None:intergenic
TTCTTCTCCGGCGAATGTTG+TGG	0.493038	7_2:-77153298	None:intergenic
GTGGCTGACCCAAAGTATGA+AGG	0.567065	7_2:+77154373	Msa1078270:CDS
AGACTGAGATGCTTTACAGA+TGG	0.571757	7_2:+77154443	Msa1078270:three_prime_UTR
ATGATTGATGACAATGATCT+GGG	0.577930	7_2:+77154289	Msa1078270:CDS
AGCACGACCACAACATTCGC+CGG	0.611769	7_2:+77153291	Msa1078270:five_prime_UTR
ATGATACCTTGAAAACGCGG+TGG	0.684438	7_2:-77153331	None:intergenic
AAATTAATATTCCCAAAACG+AGG	0.696429	7_2:-77154490	None:intergenic
GCGATGATACCTTGAAAACG+CGG	0.740904	7_2:-77153334	None:intergenic
GATAGCATATCACTATGTCG+TGG	0.751623	7_2:+77154354	Msa1078270:CDS

CRISPR-GE

badsite warning	sgRNA_sequence	Strand	Position	Region	GC_content
!!	ATAAAATAAAATAATGATGA+TGG	-	chr7_2:77153406-77153425	None:intergenic	10.0%
!!	ATAAAATAAAATAATGATGA+TGG	-	chr7_2:77153406-77153425	None:intergenic	10.0%
!!	CCTATATTACTATAAAAATT+GGG	+	chr7_2:77154043-77154062	Msa1078270:intron	15.0%
!!	CATAACAAAACAAAAAAAAA+TGG	-	chr7_2:77154557-77154576	None:intergenic	15.0%
!!	CCTATATTACTATAAAAATT+GGG	+	chr7_2:77154043-77154062	Msa1078270:intron	15.0%
!!	CATAACAAAACAAAAAAAAA+TGG	-	chr7_2:77154557-77154576	None:intergenic	15.0%
!!!	CCTAAATTTTTCAGATAATT+CGG	+	chr7_2:77153574-77153593	Msa1078270:intron	20.0%
!!!	GTTTTGATTTTAAGTTGTAA+AGG	+	chr7_2:77153641-77153660	Msa1078270:intron	20.0%
!!!	AAAGGATTTAAGATTTGATT+AGG	+	chr7_2:77153659-77153678	Msa1078270:intron	20.0%
!!	TAAAAATTGTTGACAAATGT+TGG	+	chr7_2:77153742-77153761	Msa1078270:intron	20.0%
!!	GCCTATATTACTATAAAAAT+TGG	+	chr7_2:77154042-77154061	Msa1078270:intron	20.0%
!!!	CCCAATTTTTATAGTAATAT+AGG	-	chr7_2:77154046-77154065	None:intergenic	20.0%
!!!	GAAAATCAATCTACTTTTTT+AGG	-	chr7_2:77154197-77154216	None:intergenic	20.0%
!!!	TTTTTGTTTTGTTATGCTTA+TGG	+	chr7_2:77154561-77154580	Msa1078270:three_prime_UTR	20.0%
!!!	TTTTGTTTTGTTATGCTTAT+GGG	+	chr7_2:77154562-77154581	Msa1078270:three_prime_UTR	20.0%
!!!	CCTAAATTTTTCAGATAATT+CGG	+	chr7_2:77153574-77153593	Msa1078270:intron	20.0%
!!!	GTTTTGATTTTAAGTTGTAA+AGG	+	chr7_2:77153641-77153660	Msa1078270:intron	20.0%
!!!	AAAGGATTTAAGATTTGATT+AGG	+	chr7_2:77153659-77153678	Msa1078270:intron	20.0%
!!	TAAAAATTGTTGACAAATGT+TGG	+	chr7_2:77153742-77153761	Msa1078270:intron	20.0%
!!	GCCTATATTACTATAAAAAT+TGG	+	chr7_2:77154042-77154061	Msa1078270:intron	20.0%
!!!	CCCAATTTTTATAGTAATAT+AGG	-	chr7_2:77154046-77154065	None:intergenic	20.0%
!!!	GAAAATCAATCTACTTTTTT+AGG	-	chr7_2:77154197-77154216	None:intergenic	20.0%
!!!	TTTTTGTTTTGTTATGCTTA+TGG	+	chr7_2:77154561-77154580	Msa1078270:three_prime_UTR	20.0%
!!!	TTTTGTTTTGTTATGCTTAT+GGG	+	chr7_2:77154562-77154581	Msa1078270:three_prime_UTR	20.0%
!	CCGAATTATCTGAAAAATTT+AGG	-	chr7_2:77153577-77153596	None:intergenic	25.0%
!	GAGAGAATATATGTTACAAT+TGG	+	chr7_2:77153709-77153728	Msa1078270:intron	25.0%
!!	TTTGTCAACAATTTTTAACC+AGG	-	chr7_2:77153739-77153758	None:intergenic	25.0%
!!!	TTTAAGTTGTTTTTCGAAAC+AGG	+	chr7_2:77154019-77154038	Msa1078270:intron	25.0%
!!!	TTAAGTTGTTTTTCGAAACA+GGG	+	chr7_2:77154020-77154039	Msa1078270:intron	25.0%
!	TTACTATAAAAATTGGGATC+AGG	+	chr7_2:77154049-77154068	Msa1078270:intron	25.0%
!	TACTATAAAAATTGGGATCA+GGG	+	chr7_2:77154050-77154069	Msa1078270:intron	25.0%
!!	TATAATTATGAACCTCGTTT+TGG	+	chr7_2:77154478-77154497	Msa1078270:three_prime_UTR	25.0%
!!	ATAATTATGAACCTCGTTTT+GGG	+	chr7_2:77154479-77154498	Msa1078270:three_prime_UTR	25.0%
!	AAATTAATATTCCCAAAACG+AGG	-	chr7_2:77154493-77154512	None:intergenic	25.0%
!!!	TGGGAATATTAATTTTAGCT+TGG	+	chr7_2:77154498-77154517	Msa1078270:three_prime_UTR	25.0%
!	CCGAATTATCTGAAAAATTT+AGG	-	chr7_2:77153577-77153596	None:intergenic	25.0%
!	GAGAGAATATATGTTACAAT+TGG	+	chr7_2:77153709-77153728	Msa1078270:intron	25.0%
!!	TTTGTCAACAATTTTTAACC+AGG	-	chr7_2:77153739-77153758	None:intergenic	25.0%
!!!	TTTAAGTTGTTTTTCGAAAC+AGG	+	chr7_2:77154019-77154038	Msa1078270:intron	25.0%
!!!	TTAAGTTGTTTTTCGAAACA+GGG	+	chr7_2:77154020-77154039	Msa1078270:intron	25.0%
!	TTACTATAAAAATTGGGATC+AGG	+	chr7_2:77154049-77154068	Msa1078270:intron	25.0%
!	TACTATAAAAATTGGGATCA+GGG	+	chr7_2:77154050-77154069	Msa1078270:intron	25.0%
!!	TATAATTATGAACCTCGTTT+TGG	+	chr7_2:77154478-77154497	Msa1078270:three_prime_UTR	25.0%
!!	ATAATTATGAACCTCGTTTT+GGG	+	chr7_2:77154479-77154498	Msa1078270:three_prime_UTR	25.0%
!	AAATTAATATTCCCAAAACG+AGG	-	chr7_2:77154493-77154512	None:intergenic	25.0%
!!!	TGGGAATATTAATTTTAGCT+TGG	+	chr7_2:77154498-77154517	Msa1078270:three_prime_UTR	25.0%
!!	ATTTGCATCAGAGTTTTGTA+AGG	+	chr7_2:77153246-77153265	Msa1078270:five_prime_UTR	30.0%
!	TGATTTACGAGTTTTCAGAT+CGG	+	chr7_2:77153511-77153530	Msa1078270:intron	30.0%
	TGATAAGAAGTTTAGCTAGT+TGG	+	chr7_2:77154100-77154119	Msa1078270:intron	30.0%
	ATTCAAATGTGCAACAAGAA+GGG	-	chr7_2:77154134-77154153	None:intergenic	30.0%
	AATTCAAATGTGCAACAAGA+AGG	-	chr7_2:77154135-77154154	None:intergenic	30.0%
	TTGTTGCACATTTGAATTTG+TGG	+	chr7_2:77154137-77154156	Msa1078270:intron	30.0%
!	ATTTGAATTTGTGGTCTTGA+TGG	+	chr7_2:77154146-77154165	Msa1078270:intron	30.0%
!	ATTTTGTGATATCACAAACC+AGG	+	chr7_2:77154230-77154249	Msa1078270:intron	30.0%
!	ATGATTGATGACAATGATCT+GGG	+	chr7_2:77154289-77154308	Msa1078270:CDS	30.0%
!	TCTAGTTTCCTTCATACTTT+GGG	-	chr7_2:77154384-77154403	None:intergenic	30.0%
!!	TTCTAGTTTCCTTCATACTT+TGG	-	chr7_2:77154385-77154404	None:intergenic	30.0%
!!	ATTTGCATCAGAGTTTTGTA+AGG	+	chr7_2:77153246-77153265	Msa1078270:five_prime_UTR	30.0%
!	TGATTTACGAGTTTTCAGAT+CGG	+	chr7_2:77153511-77153530	Msa1078270:intron	30.0%
	TGATAAGAAGTTTAGCTAGT+TGG	+	chr7_2:77154100-77154119	Msa1078270:intron	30.0%
	ATTCAAATGTGCAACAAGAA+GGG	-	chr7_2:77154134-77154153	None:intergenic	30.0%
	AATTCAAATGTGCAACAAGA+AGG	-	chr7_2:77154135-77154154	None:intergenic	30.0%
	TTGTTGCACATTTGAATTTG+TGG	+	chr7_2:77154137-77154156	Msa1078270:intron	30.0%
!	ATTTGAATTTGTGGTCTTGA+TGG	+	chr7_2:77154146-77154165	Msa1078270:intron	30.0%
!	ATTTTGTGATATCACAAACC+AGG	+	chr7_2:77154230-77154249	Msa1078270:intron	30.0%
!	ATGATTGATGACAATGATCT+GGG	+	chr7_2:77154289-77154308	Msa1078270:CDS	30.0%
!	TCTAGTTTCCTTCATACTTT+GGG	-	chr7_2:77154384-77154403	None:intergenic	30.0%
!!	TTCTAGTTTCCTTCATACTT+TGG	-	chr7_2:77154385-77154404	None:intergenic	30.0%
	AGAAACGAGAATTGAGAAGA+AGG	-	chr7_2:77153359-77153378	None:intergenic	35.0%
!	GATTTACGAGTTTTCAGATC+GGG	+	chr7_2:77153512-77153531	Msa1078270:intron	35.0%
!	ATTTACGAGTTTTCAGATCG+GGG	+	chr7_2:77153513-77153532	Msa1078270:intron	35.0%
	TATGTTACAATTGGTAAGCC+TGG	+	chr7_2:77153718-77153737	Msa1078270:intron	35.0%
	TCAGGCTCACAATATATCAA+AGG	-	chr7_2:77153817-77153836	None:intergenic	35.0%
	TGATATATTGTGAGCCTGAT+TGG	+	chr7_2:77153818-77153837	Msa1078270:intron	35.0%
	TCAAACACATCAATCCAATC+AGG	-	chr7_2:77153835-77153854	None:intergenic	35.0%
	TAAGCACTTGTGAAACTGTT+TGG	+	chr7_2:77153869-77153888	Msa1078270:intron	35.0%
	TGGTATGCTTGATTGTCATA+TGG	+	chr7_2:77154166-77154185	Msa1078270:intron	35.0%
	AAAAATATTCGTGAGTGACC+TGG	-	chr7_2:77154251-77154270	None:intergenic	35.0%
!	GATGATTGATGACAATGATC+TGG	+	chr7_2:77154288-77154307	Msa1078270:intron	35.0%
!	GGTTTCTTTGCCAATTTTCT+CGG	+	chr7_2:77154310-77154329	Msa1078270:CDS	35.0%
	AGAAACGAGAATTGAGAAGA+AGG	-	chr7_2:77153359-77153378	None:intergenic	35.0%
!	GATTTACGAGTTTTCAGATC+GGG	+	chr7_2:77153512-77153531	Msa1078270:intron	35.0%
!	ATTTACGAGTTTTCAGATCG+GGG	+	chr7_2:77153513-77153532	Msa1078270:intron	35.0%
	TATGTTACAATTGGTAAGCC+TGG	+	chr7_2:77153718-77153737	Msa1078270:intron	35.0%
	TCAGGCTCACAATATATCAA+AGG	-	chr7_2:77153817-77153836	None:intergenic	35.0%
	TGATATATTGTGAGCCTGAT+TGG	+	chr7_2:77153818-77153837	Msa1078270:intron	35.0%
	TCAAACACATCAATCCAATC+AGG	-	chr7_2:77153835-77153854	None:intergenic	35.0%
	TAAGCACTTGTGAAACTGTT+TGG	+	chr7_2:77153869-77153888	Msa1078270:intron	35.0%
	TGGTATGCTTGATTGTCATA+TGG	+	chr7_2:77154166-77154185	Msa1078270:intron	35.0%
	AAAAATATTCGTGAGTGACC+TGG	-	chr7_2:77154251-77154270	None:intergenic	35.0%
!	GATGATTGATGACAATGATC+TGG	+	chr7_2:77154288-77154307	Msa1078270:intron	35.0%
!	GGTTTCTTTGCCAATTTTCT+CGG	+	chr7_2:77154310-77154329	Msa1078270:CDS	35.0%
	ATGCCGACACTTATTCTTAC+AGG	+	chr7_2:77153465-77153484	Msa1078270:intron	40.0%
	GCACCTGTAAGAATAAGTGT+CGG	-	chr7_2:77153471-77153490	None:intergenic	40.0%
	CTGTAAATGCAATCCTGATG+TGG	+	chr7_2:77153773-77153792	Msa1078270:intron	40.0%
	AAAATCTCTGCAACCACATC+AGG	-	chr7_2:77153789-77153808	None:intergenic	40.0%
	GATAGCATATCACTATGTCG+TGG	+	chr7_2:77154354-77154373	Msa1078270:CDS	40.0%
	AGACTGAGATGCTTTACAGA+TGG	+	chr7_2:77154443-77154462	Msa1078270:three_prime_UTR	40.0%
	TAACTAGCACAAGAGTCTAG+CGG	-	chr7_2:77153227-77153246	None:intergenic	40.0%
	ATGCCGACACTTATTCTTAC+AGG	+	chr7_2:77153465-77153484	Msa1078270:intron	40.0%
	GCACCTGTAAGAATAAGTGT+CGG	-	chr7_2:77153471-77153490	None:intergenic	40.0%
	CTGTAAATGCAATCCTGATG+TGG	+	chr7_2:77153773-77153792	Msa1078270:intron	40.0%
	AAAATCTCTGCAACCACATC+AGG	-	chr7_2:77153789-77153808	None:intergenic	40.0%
	GATAGCATATCACTATGTCG+TGG	+	chr7_2:77154354-77154373	Msa1078270:CDS	40.0%
	AGACTGAGATGCTTTACAGA+TGG	+	chr7_2:77154443-77154462	Msa1078270:three_prime_UTR	40.0%
	ATGTTGTGGTCGTGCTTCTT+AGG	-	chr7_2:77153287-77153306	None:intergenic	45.0%
	GGAAATGGGTGTTTCTTCTC+CGG	-	chr7_2:77153313-77153332	None:intergenic	45.0%
	CTTGAAAACGCGGTGGAAAT+GGG	-	chr7_2:77153327-77153346	None:intergenic	45.0%
	ATGATACCTTGAAAACGCGG+TGG	-	chr7_2:77153334-77153353	None:intergenic	45.0%
	GCGATGATACCTTGAAAACG+CGG	-	chr7_2:77153337-77153356	None:intergenic	45.0%
	GATGAAGACGCCGAGAAAAT+TGG	-	chr7_2:77154323-77154342	None:intergenic	45.0%
	ATGTTGTGGTCGTGCTTCTT+AGG	-	chr7_2:77153287-77153306	None:intergenic	45.0%
	GGAAATGGGTGTTTCTTCTC+CGG	-	chr7_2:77153313-77153332	None:intergenic	45.0%
	CTTGAAAACGCGGTGGAAAT+GGG	-	chr7_2:77153327-77153346	None:intergenic	45.0%
	ATGATACCTTGAAAACGCGG+TGG	-	chr7_2:77153334-77153353	None:intergenic	45.0%
	GCGATGATACCTTGAAAACG+CGG	-	chr7_2:77153337-77153356	None:intergenic	45.0%
	GATGAAGACGCCGAGAAAAT+TGG	-	chr7_2:77154323-77154342	None:intergenic	45.0%
	TTCTTCTCCGGCGAATGTTG+TGG	-	chr7_2:77153301-77153320	None:intergenic	50.0%
	CCTTGAAAACGCGGTGGAAA+TGG	-	chr7_2:77153328-77153347	None:intergenic	50.0%
!	CCATTTCCACCGCGTTTTCA+AGG	+	chr7_2:77153325-77153344	Msa1078270:five_prime_UTR	50.0%
	GTGGCTGACCCAAAGTATGA+AGG	+	chr7_2:77154373-77154392	Msa1078270:CDS	50.0%
	TTCTTCTCCGGCGAATGTTG+TGG	-	chr7_2:77153301-77153320	None:intergenic	50.0%
	CCTTGAAAACGCGGTGGAAA+TGG	-	chr7_2:77153328-77153347	None:intergenic	50.0%
!	CCATTTCCACCGCGTTTTCA+AGG	+	chr7_2:77153325-77153344	Msa1078270:five_prime_UTR	50.0%
	GTGGCTGACCCAAAGTATGA+AGG	+	chr7_2:77154373-77154392	Msa1078270:CDS	50.0%
	AGCACGACCACAACATTCGC+CGG	+	chr7_2:77153291-77153310	Msa1078270:five_prime_UTR	55.0%
	CGGCGTCTTCATCTTTGTGC+TGG	+	chr7_2:77154330-77154349	Msa1078270:CDS	55.0%
	AGCACGACCACAACATTCGC+CGG	+	chr7_2:77153291-77153310	Msa1078270:five_prime_UTR	55.0%
	CGGCGTCTTCATCTTTGTGC+TGG	+	chr7_2:77154330-77154349	Msa1078270:CDS	55.0%

Chromosome	Type	Strat	End	Strand	Name
chr7_2	gene	77153227	77154596	77153227	ID=Msa1078270;Name=Msa1078270
chr7_2	mRNA	77153227	77154596	77153227	ID=Msa1078270-mRNA-1;Parent=Msa1078270;Name=Msa1078270-mRNA-1;_AED=0.05;_eAED=0.05;_QI=120\|1\|1\|1\|0\|0\|2\|194\|37
chr7_2	exon	77153227	77153346	77153227	ID=Msa1078270-mRNA-1:exon:18852;Parent=Msa1078270-mRNA-1
chr7_2	exon	77154289	77154596	77154289	ID=Msa1078270-mRNA-1:exon:18853;Parent=Msa1078270-mRNA-1
chr7_2	five_prime_UTR	77153227	77153346	77153227	ID=Msa1078270-mRNA-1:five_prime_utr;Parent=Msa1078270-mRNA-1
chr7_2	CDS	77154289	77154402	77154289	ID=Msa1078270-mRNA-1:cds;Parent=Msa1078270-mRNA-1
chr7_2	three_prime_UTR	77154403	77154596	77154403	ID=Msa1078270-mRNA-1:three_prime_utr;Parent=Msa1078270-mRNA-1

Gene Sequence	>Msa1078270 ATGATTGATGACAATGATCTGGGTTTCTTTGCCAATTTTCTCGGCGTCTTCATCTTTGTGCTGGTGATAGCATATCACTATGTCGTGGCTGACCCAAAGTATGAAGGAAACTAG
Protein sequence	>Msa1078270 MIDDNDLGFFANFLGVFIFVLVIAYHYVVADPKYEGN*