AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa0394660


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0394660 MtrunA17_Chr2g0325521 84.615 143 20 1 76 216 79 221 3.07e-83 247
Msa0394660 MtrunA17_Chr4g0041051 67.133 143 45 1 76 216 88 230 6.78e-64 198
Msa0394660 MtrunA17_Chr4g0049701 92.500 80 6 0 6 85 67 146 2.97e-50 166
Msa0394660 MtrunA17_Chr4g0049671 69.524 105 28 3 6 106 61 165 7.00e-40 144
Msa0394660 MtrunA17_Chr4g0049651 55.039 129 49 2 6 134 70 189 3.95e-37 135
Msa0394660 MtrunA17_Chr4g0045901 42.361 144 79 2 77 217 96 238 2.08e-30 113
Msa0394660 MtrunA17_Chr7g0255961 39.552 134 74 3 84 216 105 232 7.48e-20 84.7
Msa0394660 MtrunA17_Chr1g0198801 35.556 135 76 3 84 214 188 315 2.37e-18 82.0
Msa0394660 MtrunA17_Chr3g0113721 31.724 145 80 5 78 210 61 198 2.51e-16 74.7
Msa0394660 MtrunA17_Chr2g0283714 40.217 92 52 1 74 165 4 92 3.48e-16 72.4
Msa0394660 MtrunA17_Chr4g0060581 37.662 77 46 1 8 82 77 153 3.60e-12 62.4
Msa0394660 MtrunA17_Chr5g0412371 31.387 137 77 5 56 181 44 174 5.19e-12 62.8
Msa0394660 MtrunA17_Chr5g0427361 30.597 134 65 3 77 183 58 190 8.57e-12 62.8
Msa0394660 MtrunA17_Chr5g0416561 37.363 91 46 4 8 88 131 220 2.21e-11 61.2
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0394660 AT3G25710.1 37.097 124 69 2 83 202 161 279 5.12e-18 81.6
Msa0394660 AT1G68810.1 35.338 133 81 3 84 214 204 333 1.18e-17 80.9
Msa0394660 AT3G56770.1 35.043 117 71 3 78 190 67 182 6.66e-14 68.6
Msa0394660 AT2G40200.1 31.724 145 87 4 78 215 85 224 3.87e-13 67.0
Msa0394660 AT2G41130.1 32.593 135 83 3 84 216 97 225 3.91e-13 67.0

Find 31 sgRNAs with CRISPR-Local

Find 104 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
TGGCTCATCATACCGGATTT+TGG 0.172853 3_2:-9707387 Msa0394660:CDS
CTTATCTCATGATTGTCTTT+TGG 0.225833 3_2:+9706622 None:intergenic
TCACAAGAAGGTGGATTAAA+TGG 0.236239 3_2:-9706592 Msa0394660:CDS
AGTGATGCTCTAATTGAATA+AGG 0.252736 3_2:+9706565 None:intergenic
TGTGACTATTTGTGGTTTCA+TGG 0.344896 3_2:+9707329 None:intergenic
TTAAGCTAATGATCATCTTA+TGG 0.364943 3_2:-9707407 Msa0394660:CDS
AACCTCCATGTGACTATTTG+TGG 0.367739 3_2:+9707321 None:intergenic
TGTAATAACTTCAGCTAGTA+AGG 0.369694 3_2:+9706699 None:intergenic
GTGGTTTCATGGAGCGAATA+AGG 0.404640 3_2:+9707340 None:intergenic
AAGGGCAGAGAATGCAACTT+TGG 0.427512 3_2:-9706470 Msa0394660:CDS
GCTAGCAATGAGTTTCTGTA+AGG 0.438556 3_2:+9707212 None:intergenic
AGGGCAGAGAATGCAACTTT+GGG 0.451743 3_2:-9706469 Msa0394660:CDS
GTGATGCTCTAATTGAATAA+GGG 0.459428 3_2:+9706566 None:intergenic
TCATTACCATGTCTGACATC+AGG 0.466569 3_2:-9707449 None:intergenic
TAAGAAGTTCGTCCAAAATC+CGG 0.469589 3_2:+9707375 None:intergenic
GAGAGATATATGAAAGAGAA+TGG 0.473137 3_2:-9707243 Msa0394660:CDS
CATGGAGGTTAAATAGCTAC+TGG 0.492701 3_2:-9707308 Msa0394660:CDS
CATCTTATGGCTCATCATAC+CGG 0.498653 3_2:-9707394 Msa0394660:CDS
CTGACATCAGGTTTCAGCTC+TGG 0.505719 3_2:-9707437 Msa0394660:CDS
CTTATATCAGACAACAAACC+TGG 0.520795 3_2:+9706526 None:intergenic
CTATGTTGTGAACACAAACC+AGG 0.553984 3_2:-9706544 Msa0394660:CDS
ATGATCATTAGCTTAATGTA+CGG 0.579305 3_2:+9707414 None:intergenic
AATGCAGCACAAGCAAGTGA+AGG 0.583141 3_2:-9706655 Msa0394660:CDS
ACAGAAACTCATTGCTAGCA+AGG 0.589154 3_2:-9707208 Msa0394660:intron
ATGCAGCACAAGCAAGTGAA+GGG 0.608119 3_2:-9706654 Msa0394660:CDS
AGATTTGAATCACAAGAAGG+TGG 0.613421 3_2:-9706601 Msa0394660:CDS
ATAAGATTTGAATCACAAGA+AGG 0.621854 3_2:-9706604 Msa0394660:CDS
GCAGAGAATGCAACTTTGGG+AGG 0.622770 3_2:-9706466 Msa0394660:CDS
CTGAAACCTGATGTCAGACA+TGG 0.638276 3_2:+9707443 None:intergenic
TGAAACCACAAATAGTCACA+TGG 0.694501 3_2:-9707326 Msa0394660:CDS
AACCACAAATAGTCACATGG+AGG 0.755899 3_2:-9707323 Msa0394660:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! TAATAGTAAAGAAATATTTA+AGG - chr3_2:9706786-9706805 Msa0394660:intron 10.0%
!! TAATAGTAAAGAAATATTTA+AGG - chr3_2:9706786-9706805 Msa0394660:intron 10.0%
!! TAACTTAAGAAGAATTATAT+AGG - chr3_2:9706621-9706640 Msa0394660:CDS 15.0%
!! AACTTAAGAAGAATTATATA+GGG - chr3_2:9706622-9706641 Msa0394660:CDS 15.0%
!!! AATATTTAAGGTAAAGTTTT+TGG - chr3_2:9706798-9706817 Msa0394660:intron 15.0%
!!! ACTTCATTTTATGATAATAA+GGG - chr3_2:9707289-9707308 Msa0394660:CDS 15.0%
!! TAACTTAAGAAGAATTATAT+AGG - chr3_2:9706621-9706640 Msa0394660:CDS 15.0%
!! AACTTAAGAAGAATTATATA+GGG - chr3_2:9706622-9706641 Msa0394660:CDS 15.0%
!!! AATATTTAAGGTAAAGTTTT+TGG - chr3_2:9706798-9706817 Msa0394660:intron 15.0%
!!! ACTTCATTTTATGATAATAA+GGG - chr3_2:9707289-9707308 Msa0394660:CDS 15.0%
!! TTTGTCAAACTAAGTTAAAA+AGG + chr3_2:9707054-9707073 None:intergenic 20.0%
!!! CACTTCATTTTATGATAATA+AGG - chr3_2:9707288-9707307 Msa0394660:CDS 20.0%
!! TTTGTCAAACTAAGTTAAAA+AGG + chr3_2:9707054-9707073 None:intergenic 20.0%
!!! CACTTCATTTTATGATAATA+AGG - chr3_2:9707288-9707307 Msa0394660:CDS 20.0%
!! ATGATCATTAGCTTAATGTA+CGG + chr3_2:9706366-9706385 None:intergenic 25.0%
! TTAAGCTAATGATCATCTTA+TGG - chr3_2:9706370-9706389 Msa0394660:CDS 25.0%
! TTCTTTACTATTAGTACCTA+TGG + chr3_2:9706779-9706798 None:intergenic 25.0%
! ATACAAAGACATCAAAGATT+TGG - chr3_2:9706962-9706981 Msa0394660:intron 25.0%
! TCTACACAAATATACAAACA+TGG - chr3_2:9706991-9707010 Msa0394660:intron 25.0%
! ATAAGATTTGAATCACAAGA+AGG - chr3_2:9707173-9707192 Msa0394660:intron 25.0%
!! ATGATCATTAGCTTAATGTA+CGG + chr3_2:9706366-9706385 None:intergenic 25.0%
! TTAAGCTAATGATCATCTTA+TGG - chr3_2:9706370-9706389 Msa0394660:CDS 25.0%
! TTCTTTACTATTAGTACCTA+TGG + chr3_2:9706779-9706798 None:intergenic 25.0%
! ATACAAAGACATCAAAGATT+TGG - chr3_2:9706962-9706981 Msa0394660:intron 25.0%
! TCTACACAAATATACAAACA+TGG - chr3_2:9706991-9707010 Msa0394660:intron 25.0%
! ATAAGATTTGAATCACAAGA+AGG - chr3_2:9707173-9707192 Msa0394660:intron 25.0%
GAGAGATATATGAAAGAGAA+TGG - chr3_2:9706534-9706553 Msa0394660:CDS 30.0%
TGATAACTCTATATCTGCAT+TGG - chr3_2:9706662-9706681 Msa0394660:CDS 30.0%
! ACATTTAGAGTGCTTAATGA+GGG - chr3_2:9706901-9706920 Msa0394660:intron 30.0%
TGTAATAACTTCAGCTAGTA+AGG + chr3_2:9707081-9707100 None:intergenic 30.0%
! CTTATCTCATGATTGTCTTT+TGG + chr3_2:9707158-9707177 None:intergenic 30.0%
GTGATGCTCTAATTGAATAA+GGG + chr3_2:9707214-9707233 None:intergenic 30.0%
AGTGATGCTCTAATTGAATA+AGG + chr3_2:9707215-9707234 None:intergenic 30.0%
GAGAGATATATGAAAGAGAA+TGG - chr3_2:9706534-9706553 Msa0394660:CDS 30.0%
TGATAACTCTATATCTGCAT+TGG - chr3_2:9706662-9706681 Msa0394660:CDS 30.0%
! ACATTTAGAGTGCTTAATGA+GGG - chr3_2:9706901-9706920 Msa0394660:intron 30.0%
TGTAATAACTTCAGCTAGTA+AGG + chr3_2:9707081-9707100 None:intergenic 30.0%
! CTTATCTCATGATTGTCTTT+TGG + chr3_2:9707158-9707177 None:intergenic 30.0%
GTGATGCTCTAATTGAATAA+GGG + chr3_2:9707214-9707233 None:intergenic 30.0%
AGTGATGCTCTAATTGAATA+AGG + chr3_2:9707215-9707234 None:intergenic 30.0%
TAAGAAGTTCGTCCAAAATC+CGG + chr3_2:9706405-9706424 None:intergenic 35.0%
TGTGACTATTTGTGGTTTCA+TGG + chr3_2:9706451-9706470 None:intergenic 35.0%
TGAAACCACAAATAGTCACA+TGG - chr3_2:9706451-9706470 Msa0394660:CDS 35.0%
! GACATTTAGAGTGCTTAATG+AGG - chr3_2:9706900-9706919 Msa0394660:intron 35.0%
CTTAATCCTGTGCAGAAAAA+TGG - chr3_2:9706926-9706945 Msa0394660:intron 35.0%
CAAAGACATCAAAGATTTGG+AGG - chr3_2:9706965-9706984 Msa0394660:intron 35.0%
AGATTTGAATCACAAGAAGG+TGG - chr3_2:9707176-9707195 Msa0394660:intron 35.0%
!! TCACAAGAAGGTGGATTAAA+TGG - chr3_2:9707185-9707204 Msa0394660:intron 35.0%
CTTATATCAGACAACAAACC+TGG + chr3_2:9707254-9707273 None:intergenic 35.0%
AGCAAAAAACTGTCGATACT+CGG + chr3_2:9707393-9707412 None:intergenic 35.0%
TAAGAAGTTCGTCCAAAATC+CGG + chr3_2:9706405-9706424 None:intergenic 35.0%
TGTGACTATTTGTGGTTTCA+TGG + chr3_2:9706451-9706470 None:intergenic 35.0%
TGAAACCACAAATAGTCACA+TGG - chr3_2:9706451-9706470 Msa0394660:CDS 35.0%
! GACATTTAGAGTGCTTAATG+AGG - chr3_2:9706900-9706919 Msa0394660:intron 35.0%
CTTAATCCTGTGCAGAAAAA+TGG - chr3_2:9706926-9706945 Msa0394660:intron 35.0%
CAAAGACATCAAAGATTTGG+AGG - chr3_2:9706965-9706984 Msa0394660:intron 35.0%
AGATTTGAATCACAAGAAGG+TGG - chr3_2:9707176-9707195 Msa0394660:intron 35.0%
!! TCACAAGAAGGTGGATTAAA+TGG - chr3_2:9707185-9707204 Msa0394660:intron 35.0%
CTTATATCAGACAACAAACC+TGG + chr3_2:9707254-9707273 None:intergenic 35.0%
AGCAAAAAACTGTCGATACT+CGG + chr3_2:9707393-9707412 None:intergenic 35.0%
CATCTTATGGCTCATCATAC+CGG - chr3_2:9706383-9706402 Msa0394660:CDS 40.0%
AACCACAAATAGTCACATGG+AGG - chr3_2:9706454-9706473 Msa0394660:CDS 40.0%
AACCTCCATGTGACTATTTG+TGG + chr3_2:9706459-9706478 None:intergenic 40.0%
CATGGAGGTTAAATAGCTAC+TGG - chr3_2:9706469-9706488 Msa0394660:CDS 40.0%
GCTAGCAATGAGTTTCTGTA+AGG + chr3_2:9706568-9706587 None:intergenic 40.0%
ACAGAAACTCATTGCTAGCA+AGG - chr3_2:9706569-9706588 Msa0394660:CDS 40.0%
TTCTACCTGTCTTAGTCTCA+TGG - chr3_2:9706876-9706895 Msa0394660:intron 40.0%
!! GTTTCTCCATTTTTCTGCAC+AGG + chr3_2:9706935-9706954 None:intergenic 40.0%
CTATGTTGTGAACACAAACC+AGG - chr3_2:9707233-9707252 Msa0394660:CDS 40.0%
!!! GAGTATCGACAGTTTTTTGC+TGG - chr3_2:9707392-9707411 Msa0394660:CDS 40.0%
CATCTTATGGCTCATCATAC+CGG - chr3_2:9706383-9706402 Msa0394660:CDS 40.0%
AACCACAAATAGTCACATGG+AGG - chr3_2:9706454-9706473 Msa0394660:CDS 40.0%
AACCTCCATGTGACTATTTG+TGG + chr3_2:9706459-9706478 None:intergenic 40.0%
CATGGAGGTTAAATAGCTAC+TGG - chr3_2:9706469-9706488 Msa0394660:CDS 40.0%
GCTAGCAATGAGTTTCTGTA+AGG + chr3_2:9706568-9706587 None:intergenic 40.0%
ACAGAAACTCATTGCTAGCA+AGG - chr3_2:9706569-9706588 Msa0394660:CDS 40.0%
TTCTACCTGTCTTAGTCTCA+TGG - chr3_2:9706876-9706895 Msa0394660:intron 40.0%
!! GTTTCTCCATTTTTCTGCAC+AGG + chr3_2:9706935-9706954 None:intergenic 40.0%
CTATGTTGTGAACACAAACC+AGG - chr3_2:9707233-9707252 Msa0394660:CDS 40.0%
!!! GAGTATCGACAGTTTTTTGC+TGG - chr3_2:9707392-9707411 Msa0394660:CDS 40.0%
! TGGCTCATCATACCGGATTT+TGG - chr3_2:9706390-9706409 Msa0394660:CDS 45.0%
GTGGTTTCATGGAGCGAATA+AGG + chr3_2:9706440-9706459 None:intergenic 45.0%
! TCCTTTGTCGCTTCTACATC+AGG + chr3_2:9706754-9706773 None:intergenic 45.0%
GAAGCGACAAAGGAAACCAT+AGG - chr3_2:9706760-9706779 Msa0394660:intron 45.0%
TGTCACCATGAGACTAAGAC+AGG + chr3_2:9706884-9706903 None:intergenic 45.0%
AATGCAGCACAAGCAAGTGA+AGG - chr3_2:9707122-9707141 Msa0394660:intron 45.0%
ATGCAGCACAAGCAAGTGAA+GGG - chr3_2:9707123-9707142 Msa0394660:intron 45.0%
AAGGGCAGAGAATGCAACTT+TGG - chr3_2:9707307-9707326 Msa0394660:CDS 45.0%
AGGGCAGAGAATGCAACTTT+GGG - chr3_2:9707308-9707327 Msa0394660:CDS 45.0%
! TGGCTCATCATACCGGATTT+TGG - chr3_2:9706390-9706409 Msa0394660:CDS 45.0%
GTGGTTTCATGGAGCGAATA+AGG + chr3_2:9706440-9706459 None:intergenic 45.0%
! TCCTTTGTCGCTTCTACATC+AGG + chr3_2:9706754-9706773 None:intergenic 45.0%
GAAGCGACAAAGGAAACCAT+AGG - chr3_2:9706760-9706779 Msa0394660:intron 45.0%
TGTCACCATGAGACTAAGAC+AGG + chr3_2:9706884-9706903 None:intergenic 45.0%
AATGCAGCACAAGCAAGTGA+AGG - chr3_2:9707122-9707141 Msa0394660:intron 45.0%
ATGCAGCACAAGCAAGTGAA+GGG - chr3_2:9707123-9707142 Msa0394660:intron 45.0%
AAGGGCAGAGAATGCAACTT+TGG - chr3_2:9707307-9707326 Msa0394660:CDS 45.0%
AGGGCAGAGAATGCAACTTT+GGG - chr3_2:9707308-9707327 Msa0394660:CDS 45.0%
CTGACATCAGGTTTCAGCTC+TGG - chr3_2:9706340-9706359 Msa0394660:CDS 50.0%
GCCTGATGTAGAAGCGACAA+AGG - chr3_2:9706750-9706769 Msa0394660:intron 50.0%
GCAGAGAATGCAACTTTGGG+AGG - chr3_2:9707311-9707330 Msa0394660:CDS 50.0%
CTGACATCAGGTTTCAGCTC+TGG - chr3_2:9706340-9706359 Msa0394660:CDS 50.0%
GCCTGATGTAGAAGCGACAA+AGG - chr3_2:9706750-9706769 Msa0394660:intron 50.0%
GCAGAGAATGCAACTTTGGG+AGG - chr3_2:9707311-9707330 Msa0394660:CDS 50.0%
Chromosome Type Strat End Strand Name
chr3_2 gene 9706336 9707463 9706336 ID=Msa0394660;Name=Msa0394660
chr3_2 mRNA 9706336 9707463 9706336 ID=Msa0394660-mRNA-1;Parent=Msa0394660;Name=Msa0394660-mRNA-1;_AED=0.18;_eAED=0.18;_QI=0|0|0|1|1|1|2|0|217
chr3_2 exon 9707209 9707463 9707209 ID=Msa0394660-mRNA-1:exon:2541;Parent=Msa0394660-mRNA-1
chr3_2 exon 9706336 9706734 9706336 ID=Msa0394660-mRNA-1:exon:2540;Parent=Msa0394660-mRNA-1
chr3_2 CDS 9707209 9707463 9707209 ID=Msa0394660-mRNA-1:cds;Parent=Msa0394660-mRNA-1
chr3_2 CDS 9706336 9706734 9706336 ID=Msa0394660-mRNA-1:cds;Parent=Msa0394660-mRNA-1
Gene Sequence

>Msa0394660

ATGTCTGACATCAGGTTTCAGCTCTGGCCGTACATTAAGCTAATGATCATCTTATGGCTCATCATACCGGATTTTGGACGAACTTCTTATGTTTATAACTACCTTATTCGCTCCATGAAACCACAAATAGTCACATGGAGGTTAAATAGCTACTGGAGAGATTGTTTTGCTGAGAAAGATAACTTTTTACTGCATGCAGAGAGATATATGAAAGAGAATGGAACTGAAACCTTACAGAAACTCATTGCTAGCAAGTTTGACAAAACATCCTTACTAGCTGAAGTTATTACACATTTGAAAGAGCTGAAAACAAATGCAGCACAAGCAAGTGAAGGGTTAATTATTCCAAAAGACAATCATGAGATAAGATTTGAATCACAAGAAGGTGGATTAAATGGTTTCCCTTATTCAATTAGAGCATCACTATGTTGTGAACACAAACCAGGTTTGTTGTCTGATATAAGAAAAGCACTTAATGCACTTCATTTTATGATAATAAGGGCAGAGAATGCAACTTTGGGAGGTAGAATGAAGAATATCTTTGTCATAATTAGCTGCAAAGAACAGAATTTTGAAGATGCCGAGTATCGACAGTTTTTTGCTGGATCTGTTCGTCAAGCTCTTAGATCTGTACATGATAGATTTTCTAATTAA

Protein sequence

>Msa0394660

MSDIRFQLWPYIKLMIILWLIIPDFGRTSYVYNYLIRSMKPQIVTWRLNSYWRDCFAEKDNFLLHAERYMKENGTETLQKLIASKFDKTSLLAEVITHLKELKTNAAQASEGLIIPKDNHEIRFESQEGGLNGFPYSIRASLCCEHKPGLLSDIRKALNALHFMIIRAENATLGGRMKNIFVIISCKEQNFEDAEYRQFFAGSVRQALRSVHDRFSN*