AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa0492580

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0492580 MtrunA17_Chr3g0085331 72.785 158 15 1 1 130 92 249 2.79e-64 211
Msa0492580 MtrunA17_Chr3g0087541 72.152 158 16 1 1 130 92 249 4.03e-64 211
Msa0492580 MtrunA17_Chr3g0085501 72.152 158 16 1 1 130 92 249 8.27e-64 210
Msa0492580 MtrunA17_Chr3g0085801 75.316 158 11 1 1 130 94 251 1.12e-57 192
Msa0492580 MtrunA17_Chr3g0085471 63.291 158 30 1 1 130 92 249 5.48e-52 176
Msa0492580 MtrunA17_Chr3g0084031 55.063 158 42 2 2 131 96 252 1.09e-44 155
Msa0492580 MtrunA17_Chr3g0085451 58.228 158 10 2 1 130 92 221 7.03e-44 153
Msa0492580 MtrunA17_Chr3g0085561 67.742 124 12 1 35 130 95 218 1.17e-43 152
Msa0492580 MtrunA17_Chr3g0082791 53.595 153 42 2 6 130 98 249 6.46e-41 144
Msa0492580 MtrunA17_Chr3g0084081 53.548 155 43 2 4 130 96 249 8.25e-41 144
Msa0492580 MtrunA17_Chr3g0084051 52.866 157 45 2 2 130 94 249 1.28e-39 141
Msa0492580 MtrunA17_Chr3g0085421 50.323 155 48 2 4 130 20 173 9.09e-39 131
Msa0492580 MtrunA17_Chr3g0084061 49.375 160 42 3 2 133 94 242 5.72e-35 127
Msa0492580 MtrunA17_Chr3g0085651 50.000 140 41 2 19 130 102 240 1.37e-31 118
Msa0492580 MtrunA17_Chr3g0086221 51.429 140 35 3 19 130 96 230 2.65e-30 114
Msa0492580 MtrunA17_Chr3g0086621 50.714 140 37 3 19 130 96 231 1.31e-29 112
Msa0492580 MtrunA17_Chr3g0087701 54.478 134 32 2 25 130 101 233 4.85e-29 110
Msa0492580 MtrunA17_Chr7g0221481 48.148 135 41 2 25 130 101 235 1.10e-28 109
Msa0492580 MtrunA17_Chr3g0087581 47.368 133 42 1 26 130 102 234 1.54e-28 109
Msa0492580 MtrunA17_Chr3g0086161 48.571 140 40 3 19 130 96 231 6.51e-28 107
Msa0492580 MtrunA17_Chr3g0086151 49.286 140 39 3 19 130 96 231 1.14e-27 107
Msa0492580 MtrunA17_Chr3g0086181 49.286 140 39 3 19 130 96 231 1.15e-27 107
Msa0492580 MtrunA17_Chr3g0085721 42.581 155 63 3 2 130 66 220 2.15e-25 99.0
Msa0492580 MtrunA17_Chr3g0085751 41.558 154 65 5 1 130 76 228 1.48e-24 98.2
Msa0492580 MtrunA17_Chr3g0085521 45.926 135 55 3 19 135 96 230 1.89e-24 97.8
Msa0492580 MtrunA17_Chr3g0091011 43.571 140 50 3 26 136 103 242 4.18e-22 90.9
Msa0492580 MtrunA17_Chr3g0085091 41.045 134 50 2 26 130 103 236 1.58e-21 89.4
Msa0492580 MtrunA17_Chr3g0086741 79.245 53 11 0 78 130 205 257 2.43e-21 89.0
Msa0492580 MtrunA17_Chr3g0085551 62.857 70 24 1 73 140 66 135 1.45e-20 86.7
Msa0492580 MtrunA17_Chr1g0189501 60.938 64 25 0 69 132 18 81 1.76e-20 80.5
Msa0492580 MtrunA17_Chr3g0087731 93.023 43 3 0 89 131 1 43 4.99e-20 85.1
Msa0492580 MtrunA17_Chr1g0164611 32.515 163 80 4 4 136 95 257 8.40e-20 84.3
Msa0492580 MtrunA17_Chr3g0082101 32.515 163 80 4 4 136 95 257 8.70e-20 84.3
Msa0492580 MtrunA17_Chr3g0089931 50.685 73 31 1 62 134 162 229 1.18e-19 84.0
Msa0492580 MtrunA17_Chr3g0085171 43.089 123 54 3 24 130 95 217 3.03e-19 82.8
Msa0492580 MtrunA17_Chr1g0164631 38.281 128 56 5 31 138 121 245 4.12e-19 82.4
Msa0492580 MtrunA17_Chr3g0089871 56.667 60 26 0 75 134 185 244 1.21e-18 81.3
Msa0492580 MtrunA17_Chr3g0085161 66.154 65 19 2 71 132 48 112 1.47e-18 80.9
Msa0492580 MtrunA17_Chr3g0082131 35.526 152 73 5 4 130 95 246 2.87e-18 80.1
Msa0492580 MtrunA17_Chr4g0025391 56.667 60 26 0 75 134 184 243 4.12e-18 79.7
Msa0492580 MtrunA17_Chr3g0085111 35.252 139 68 1 24 140 102 240 4.38e-18 79.7
Msa0492580 MtrunA17_Chr3g0090401 30.058 173 88 4 1 140 296 468 5.78e-18 79.3
Msa0492580 MtrunA17_Chr8g0352861 35.220 159 72 4 3 130 96 254 8.93e-18 78.6
Msa0492580 MtrunA17_Chr3g0092261 50.746 67 33 0 68 134 222 288 1.27e-17 78.2
Msa0492580 MtrunA17_Chr3g0089911 55.000 60 27 0 75 134 184 243 1.33e-17 78.2
Msa0492580 MtrunA17_Chr3g0082311 55.172 58 26 0 73 130 190 247 1.37e-17 78.2
Msa0492580 MtrunA17_Chr1g0169901 55.000 60 26 1 72 130 177 236 1.59e-17 77.8
Msa0492580 MtrunA17_Chr3g0085131 38.571 140 63 3 24 140 102 241 1.70e-17 77.8
Msa0492580 MtrunA17_Chr3g0090141 50.769 65 32 0 76 140 193 257 1.71e-17 77.8
Msa0492580 MtrunA17_Chr3g0089781 50.794 63 31 0 74 136 191 253 1.78e-17 77.8
Msa0492580 MtrunA17_Chr1g0164581 37.778 135 69 4 4 130 95 222 1.79e-17 77.8
Msa0492580 MtrunA17_Chr3g0089541 54.386 57 26 0 74 130 195 251 1.88e-17 77.8
Msa0492580 MtrunA17_Chr3g0090691 33.333 147 75 5 6 129 100 246 2.42e-17 77.4
Msa0492580 MtrunA17_Chr3g0082891 41.739 115 45 4 38 130 122 236 2.73e-17 77.4
Msa0492580 MtrunA17_Chr3g0085701 68.966 58 18 0 74 131 175 232 2.73e-17 77.4
Msa0492580 MtrunA17_Chr3g0089761 52.381 63 29 1 68 130 186 247 2.88e-17 77.4
Msa0492580 MtrunA17_Chr8g0348931 55.932 59 26 0 76 134 193 251 2.93e-17 77.0
Msa0492580 MtrunA17_Chr3g0091061 33.333 147 75 5 6 129 100 246 3.26e-17 77.0
Msa0492580 MtrunA17_Chr3g0082871 41.739 115 45 4 38 130 122 236 3.43e-17 77.0
Msa0492580 MtrunA17_Chr3g0087841 50.000 66 33 0 75 140 192 257 3.72e-17 77.0
Msa0492580 MtrunA17_Chr3g0090651 32.653 147 76 5 6 129 100 246 3.74e-17 77.0
Msa0492580 MtrunA17_Chr3g0089991 54.545 55 25 0 76 130 197 251 3.78e-17 77.0
Msa0492580 MtrunA17_Chr3g0082881 52.239 67 31 1 65 130 170 236 3.91e-17 76.6
Msa0492580 MtrunA17_Chr3g0091041 32.237 152 70 6 6 129 100 246 4.67e-17 76.6
Msa0492580 MtrunA17_Chr3g0090371 47.761 67 35 0 74 140 191 257 4.76e-17 76.6
Msa0492580 MtrunA17_Chr3g0089431 52.632 57 27 0 74 130 195 251 5.22e-17 76.3
Msa0492580 MtrunA17_Chr3g0082811 41.739 115 45 4 38 130 122 236 5.49e-17 76.3
Msa0492580 MtrunA17_Chr3g0089501 52.381 63 29 1 68 130 184 245 5.88e-17 76.3
Msa0492580 MtrunA17_Chr3g0089601 52.381 63 29 1 68 130 184 245 6.08e-17 76.3
Msa0492580 MtrunA17_Chr8g0352831 34.568 162 70 5 4 130 97 257 7.28e-17 76.3
Msa0492580 MtrunA17_Chr3g0091081 31.973 147 77 5 6 129 100 246 7.72e-17 75.9
Msa0492580 MtrunA17_Chr3g0089961 52.727 55 26 0 76 130 193 247 8.79e-17 75.9
Msa0492580 MtrunA17_Chr3g0082381 51.724 58 28 0 73 130 189 246 9.02e-17 75.1
Msa0492580 MtrunA17_Chr3g0089421 52.727 55 26 0 76 130 192 246 1.00e-16 75.9
Msa0492580 MtrunA17_Chr8g0392521 51.667 60 29 0 75 134 158 217 1.09e-16 74.7
Msa0492580 MtrunA17_Chr3g0089651 46.032 63 34 0 74 136 195 257 1.21e-16 75.5
Msa0492580 MtrunA17_Chr2g0309721 47.619 63 33 0 74 136 191 253 1.86e-16 75.1
Msa0492580 MtrunA17_Chr1g0164391 53.448 58 27 0 73 130 190 247 2.14e-16 74.7
Msa0492580 MtrunA17_Chr3g0089471 47.692 65 34 0 76 140 194 258 2.24e-16 74.7
Msa0492580 MtrunA17_Chr3g0089811 47.541 61 32 0 76 136 193 253 2.35e-16 74.7
Msa0492580 MtrunA17_Chr3g0082321 53.448 58 27 0 73 130 190 247 2.43e-16 74.7
Msa0492580 MtrunA17_Chr1g0147141 52.542 59 28 0 72 130 193 251 3.01e-16 74.3
Msa0492580 MtrunA17_Chr3g0089211 49.091 55 28 0 76 130 240 294 5.57e-16 73.6
Msa0492580 MtrunA17_Chr3g0089251 49.091 55 28 0 76 130 222 276 6.09e-16 73.6
Msa0492580 MtrunA17_Chr3g0090431 47.541 61 32 0 76 136 65 125 7.40e-16 73.2
Msa0492580 MtrunA17_Chr3g0089591 49.091 55 28 0 76 130 198 252 9.20e-16 72.8
Msa0492580 MtrunA17_Chr3g0092241 45.902 61 33 0 76 136 193 253 1.91e-15 72.0
Msa0492580 MtrunA17_Chr3g0086091 78.571 42 9 0 89 130 1 42 1.93e-15 72.0
Msa0492580 MtrunA17_Chr6g0484551 31.469 143 71 4 15 130 107 249 1.98e-15 72.0
Msa0492580 MtrunA17_Chr2g0308491 48.214 56 29 0 78 133 15 70 2.12e-15 70.5
Msa0492580 MtrunA17_Chr3g0084971 51.562 64 31 0 73 136 180 243 4.03e-15 71.2
Msa0492580 MtrunA17_Chr7g0248531 28.750 160 91 5 1 137 83 242 6.36e-15 70.5
Msa0492580 MtrunA17_Chr3g0082351 50.000 58 29 0 73 130 189 246 8.01e-15 70.1
Msa0492580 MtrunA17_Chr3g0082391 48.276 58 30 0 73 130 191 248 1.89e-14 68.9
Msa0492580 MtrunA17_Chr8g0338251 50.769 65 32 0 70 134 168 232 5.70e-14 67.8
Msa0492580 MtrunA17_Chr4g0020501 29.496 139 73 3 22 135 68 206 7.26e-14 67.4
Msa0492580 MtrunA17_Chr5g0404741 56.863 51 22 0 80 130 191 241 7.78e-14 67.4
Msa0492580 MtrunA17_Chr4g0051651 52.727 55 26 0 76 130 213 267 2.61e-13 65.9
Msa0492580 MtrunA17_Chr3g0113791 43.333 60 34 0 78 137 179 238 2.94e-13 65.9
Msa0492580 MtrunA17_Chr5g0415471 60.784 51 20 0 80 130 195 245 3.09e-13 65.9
Msa0492580 MtrunA17_Chr3g0113481 43.333 60 34 0 78 137 179 238 3.98e-13 65.5
Msa0492580 MtrunA17_Chr8g0338261 52.542 59 28 0 76 134 188 246 4.27e-13 65.1
Msa0492580 MtrunA17_Chr8g0351821 50.000 62 29 1 71 130 171 232 6.69e-13 64.7
Msa0492580 MtrunA17_Chr8g0338361 41.667 72 42 0 70 141 47 118 7.23e-13 64.3
Msa0492580 MtrunA17_Chr2g0299101 51.852 54 26 0 77 130 188 241 1.06e-12 63.9
Msa0492580 MtrunA17_Chr2g0299121 46.032 63 34 0 68 130 179 241 1.39e-12 63.9
Msa0492580 MtrunA17_Chr3g0101271 47.541 61 32 0 80 140 175 235 1.55e-12 63.5
Msa0492580 MtrunA17_Chr3g0101241 47.541 61 32 0 80 140 175 235 1.58e-12 63.5
Msa0492580 MtrunA17_Chr3g0083721 47.368 57 30 0 76 132 144 200 1.73e-12 63.5
Msa0492580 MtrunA17_Chr4g0008461 47.619 63 33 0 68 130 147 209 1.75e-12 63.5
Msa0492580 MtrunA17_Chr5g0415491 50.000 54 27 0 80 133 50 103 1.78e-12 62.0
Msa0492580 MtrunA17_Chr2g0316291 49.180 61 27 1 74 134 191 247 1.93e-12 63.5
Msa0492580 MtrunA17_Chr6g0467961 47.273 55 29 0 80 134 182 236 2.14e-12 63.2
Msa0492580 MtrunA17_Chr1g0169951 55.102 49 22 0 73 121 192 240 2.49e-12 63.2
Msa0492580 MtrunA17_Chr5g0414311 52.830 53 25 0 78 130 195 247 2.85e-12 62.8
Msa0492580 MtrunA17_Chr6g0468141 45.614 57 31 0 78 134 84 140 3.57e-12 62.0
Msa0492580 MtrunA17_Chr5g0415511 49.057 53 27 0 78 130 191 243 3.93e-12 62.4
Msa0492580 MtrunA17_Chr3g0083751 52.941 51 24 0 80 130 181 231 4.22e-12 62.4
Msa0492580 MtrunA17_Chr6g0468451 47.273 55 29 0 80 134 182 236 4.45e-12 62.4
Msa0492580 MtrunA17_Chr7g0257541 49.180 61 30 1 72 131 192 252 4.51e-12 62.4
Msa0492580 MtrunA17_Chr2g0299091 44.444 63 35 0 68 130 153 215 4.62e-12 62.4
Msa0492580 MtrunA17_Chr6g0468121 47.273 55 29 0 80 134 183 237 4.70e-12 62.4
Msa0492580 MtrunA17_Chr4g0037421 52.273 44 21 0 74 117 221 264 4.97e-12 62.0
Msa0492580 MtrunA17_Chr8g0338621 43.750 64 36 0 70 133 183 246 5.04e-12 62.0
Msa0492580 MtrunA17_Chr3g0078361 53.333 45 21 0 86 130 1 45 5.10e-12 62.0
Msa0492580 MtrunA17_Chr2g0282991 50.000 54 27 0 77 130 192 245 5.48e-12 62.0
Msa0492580 MtrunA17_Chr7g0248221 50.000 62 30 1 71 131 182 243 7.36e-12 61.6
Msa0492580 MtrunA17_Chr6g0468021 45.455 55 30 0 80 134 182 236 7.62e-12 61.6
Msa0492580 MtrunA17_Chr5g0446611 50.943 53 26 0 78 130 196 248 8.69e-12 61.6
Msa0492580 MtrunA17_Chr1g0189551 47.541 61 31 1 71 130 209 269 8.70e-12 61.6
Msa0492580 MtrunA17_Chr6g0470011 49.020 51 26 0 80 130 182 232 9.99e-12 61.2
Msa0492580 MtrunA17_Chr8g0338671 44.615 65 35 1 69 133 65 128 1.01e-11 61.2
Msa0492580 MtrunA17_Chr7g0248431 50.000 62 30 1 71 131 182 243 1.04e-11 61.2
Msa0492580 MtrunA17_Chr5g0414201 52.941 51 24 0 80 130 193 243 1.28e-11 60.8
Msa0492580 MtrunA17_Chr7g0257261 51.020 49 24 0 73 121 190 238 1.43e-11 60.1
Msa0492580 MtrunA17_Chr6g0456121 42.373 59 34 0 73 131 173 231 1.47e-11 60.8
Msa0492580 MtrunA17_Chr7g0257201 51.020 49 24 0 73 121 190 238 1.51e-11 60.8
Msa0492580 MtrunA17_Chr6g0470021 49.020 51 26 0 80 130 73 123 1.57e-11 60.8
Msa0492580 MtrunA17_Chr6g0470081 49.020 51 26 0 80 130 182 232 1.62e-11 60.8
Msa0492580 MtrunA17_Chr5g0414231 49.057 53 27 0 78 130 191 243 1.99e-11 60.5
Msa0492580 MtrunA17_Chr6g0475491 44.615 65 35 1 69 133 183 246 2.18e-11 60.5
Msa0492580 MtrunA17_Chr7g0257551 53.061 49 23 0 73 121 116 164 2.26e-11 60.1
Msa0492580 MtrunA17_Chr6g0467911 47.059 51 27 0 80 130 182 232 2.41e-11 60.1
Msa0492580 MtrunA17_Chr2g0282981 47.170 53 28 0 78 130 193 245 2.62e-11 60.1
Msa0492580 MtrunA17_Chr8g0337891 43.939 66 32 2 69 134 182 242 2.70e-11 60.1
Msa0492580 MtrunA17_Chr1g0155991 52.941 51 24 0 80 130 177 227 2.73e-11 60.1
Msa0492580 MtrunA17_Chr6g0468101 50.980 51 25 0 80 130 181 231 3.26e-11 59.7
Msa0492580 MtrunA17_Chr3g0101331 46.296 54 29 0 80 133 199 252 3.34e-11 59.7
Msa0492580 MtrunA17_Chr5g0430511 44.444 54 30 0 80 133 181 234 3.78e-11 59.7
Msa0492580 MtrunA17_Chr1g0155321 50.980 51 25 0 80 130 175 225 4.01e-11 59.7
Msa0492580 MtrunA17_Chr5g0430721 60.465 43 17 0 80 122 221 263 4.02e-11 59.7
Msa0492580 MtrunA17_Chr3g0100801 44.118 68 34 1 67 130 145 212 4.13e-11 59.7
Msa0492580 MtrunA17_Chr3g0083591 43.836 73 34 2 72 137 153 225 4.26e-11 59.7
Msa0492580 MtrunA17_Chr5g0409971 39.080 87 48 2 52 133 152 238 4.26e-11 59.7
Msa0492580 MtrunA17_Chr3g0123111 44.262 61 34 0 70 130 171 231 4.33e-11 59.7
Msa0492580 MtrunA17_Chr3g0087301 49.020 51 26 0 80 130 193 243 4.80e-11 59.3
Msa0492580 MtrunA17_Chr3g0101401 32.174 115 69 3 23 133 59 168 4.98e-11 57.8
Msa0492580 MtrunA17_Chr6g0468401 49.057 53 27 0 78 130 179 231 5.92e-11 58.9
Msa0492580 MtrunA17_Chr6g0469991 45.283 53 29 0 78 130 180 232 6.03e-11 58.9
Msa0492580 MtrunA17_Chr2g0299151 29.921 127 65 2 29 133 107 231 7.05e-11 58.9
Msa0492580 MtrunA17_Chr3g0091971 41.379 58 34 0 76 133 169 226 8.69e-11 58.5
Msa0492580 MtrunA17_Chr3g0084811 55.102 49 21 1 73 121 344 391 9.67e-11 58.5
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0492580 AT3G14470.1 31.783 129 52 2 29 129 127 247 1.82e-12 63.9
Msa0492580 AT3G14470.2 31.783 129 52 2 29 129 127 247 1.82e-12 63.9
Msa0492580 AT3G14460.1 45.161 62 33 1 80 141 195 255 1.01e-11 61.6

Find 35 sgRNAs with CRISPR-Local

Find 42 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
ACCACTTACCACCAATTTAT+TGG 0.142940 3_4:+16414823 Msa0492580:CDS
AAACCGACTATGCTGATTAT+TGG 0.261123 3_4:-16415110 None:intergenic
CCACTTACCACCAATTTATT+GGG 0.276913 3_4:+16414824 Msa0492580:CDS
CTACAAATCCATTTGAATCT+AGG 0.304525 3_4:+16414864 Msa0492580:CDS
TGAAACCAAACCCAATAAAT+TGG 0.310269 3_4:-16414834 None:intergenic
TACCACCAATTTATTGGGTT+TGG 0.326089 3_4:+16414829 Msa0492580:CDS
TCTGAATCTTCTGATGTTGT+TGG 0.342870 3_4:+16415224 Msa0492580:CDS
GCAGGCATGACAGTGGCAAC+CGG 0.372849 3_4:+16415084 Msa0492580:intron
ATAATCAGCATAGTCGGTTT+AGG 0.390635 3_4:+16415113 Msa0492580:CDS
TTCTAAGTCATTTAATTCTT+CGG 0.391207 3_4:+16415261 Msa0492580:CDS
TAAATGACTTAGAATTGCTT+TGG 0.392244 3_4:-16415252 None:intergenic
ATCTAGGCTCTATGAACAAC+TGG 0.415139 3_4:+16414880 Msa0492580:CDS
GCAAAGCAAAAGAAAGAGTT+GGG 0.442661 3_4:+16414920 Msa0492580:CDS
CCCAATAAATTGGTGGTAAG+TGG 0.460050 3_4:-16414824 None:intergenic
AAGCCTTCATTACTAGCAAT+AGG 0.461245 3_4:-16414956 None:intergenic
TCATAGAGCCTAGATTCAAA+TGG 0.474318 3_4:-16414872 None:intergenic
GCTCCTATTGCTAGTAATGA+AGG 0.482807 3_4:+16414953 Msa0492580:CDS
TGCAAAGCAAAAGAAAGAGT+TGG 0.487482 3_4:+16414919 Msa0492580:CDS
CAAAAGAAAGAGTTGGGATT+GGG 0.497323 3_4:+16414926 Msa0492580:CDS
TCATTTAATTCTTCGGCAGA+TGG 0.499541 3_4:+16415268 Msa0492580:CDS
GCAAAAGAAAGAGTTGGGAT+TGG 0.521282 3_4:+16414925 Msa0492580:CDS
CATAGTCGGTTTAGGAGGGA+TGG 0.542876 3_4:+16415121 Msa0492580:CDS
ATAGTCGGTTTAGGAGGGAT+GGG 0.556586 3_4:+16415122 Msa0492580:CDS
ATCAGCATAGTCGGTTTAGG+AGG 0.561291 3_4:+16415116 Msa0492580:CDS
AACCAAACCCAATAAATTGG+TGG 0.564098 3_4:-16414831 None:intergenic
CAGGCATGACAGTGGCAACC+GGG 0.566213 3_4:+16415085 Msa0492580:intron
CATTGTACACAAGCTTAGCA+AGG 0.568201 3_4:-16415154 None:intergenic
GTACAATGACAACAAGATAG+AGG 0.570662 3_4:+16415169 Msa0492580:CDS
CTATGCTGATTATTGGAACC+CGG 0.573752 3_4:-16415103 None:intergenic
GTACACAAGCTTAGCAAGGG+TGG 0.598583 3_4:-16415150 None:intergenic
GTTCCAATAATCAGCATAGT+CGG 0.606616 3_4:+16415107 Msa0492580:CDS
TAGTCGGTTTAGGAGGGATG+GGG 0.630822 3_4:+16415123 Msa0492580:CDS
TCAGCATAGTCGGTTTAGGA+GGG 0.636297 3_4:+16415117 Msa0492580:CDS
TTTACTTGCAGGCATGACAG+TGG 0.639300 3_4:+16415077 Msa0492580:intron
ATTGTACACAAGCTTAGCAA+GGG 0.651513 3_4:-16415153 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! TTCTAAGTCATTTAATTCTT+CGG + chr3_4:16415261-16415280 Msa0492580:CDS 20.0%
!! TTAATCAAGTTTTTACTTGC+AGG + chr3_4:16415066-16415085 Msa0492580:intron 25.0%
! TAAATGACTTAGAATTGCTT+TGG - chr3_4:16415255-16415274 None:intergenic 25.0%
TGAAACCAAACCCAATAAAT+TGG - chr3_4:16414837-16414856 None:intergenic 30.0%
CTACAAATCCATTTGAATCT+AGG + chr3_4:16414864-16414883 Msa0492580:CDS 30.0%
! GTAGATGACAATCTTTTTGA+AGG - chr3_4:16414995-16415014 None:intergenic 30.0%
ATATGGTAGAGATGATGATA+AGG + chr3_4:16415038-16415057 Msa0492580:intron 30.0%
ACCACTTACCACCAATTTAT+TGG + chr3_4:16414823-16414842 Msa0492580:CDS 35.0%
CCACTTACCACCAATTTATT+GGG + chr3_4:16414824-16414843 Msa0492580:CDS 35.0%
TACCACCAATTTATTGGGTT+TGG + chr3_4:16414829-16414848 Msa0492580:CDS 35.0%
AACCAAACCCAATAAATTGG+TGG - chr3_4:16414834-16414853 None:intergenic 35.0%
TCATAGAGCCTAGATTCAAA+TGG - chr3_4:16414875-16414894 None:intergenic 35.0%
TGCAAAGCAAAAGAAAGAGT+TGG + chr3_4:16414919-16414938 Msa0492580:CDS 35.0%
GCAAAGCAAAAGAAAGAGTT+GGG + chr3_4:16414920-16414939 Msa0492580:CDS 35.0%
CAAAAGAAAGAGTTGGGATT+GGG + chr3_4:16414926-16414945 Msa0492580:CDS 35.0%
AAGCCTTCATTACTAGCAAT+AGG - chr3_4:16414959-16414978 None:intergenic 35.0%
GTTCCAATAATCAGCATAGT+CGG + chr3_4:16415107-16415126 Msa0492580:CDS 35.0%
! AAACCGACTATGCTGATTAT+TGG - chr3_4:16415113-16415132 None:intergenic 35.0%
! ATAATCAGCATAGTCGGTTT+AGG + chr3_4:16415113-16415132 Msa0492580:CDS 35.0%
ATTGTACACAAGCTTAGCAA+GGG - chr3_4:16415156-16415175 None:intergenic 35.0%
GTACAATGACAACAAGATAG+AGG + chr3_4:16415169-16415188 Msa0492580:CDS 35.0%
! AGCATTTTGAGCTTAAAGCA+TGG + chr3_4:16415192-16415211 Msa0492580:CDS 35.0%
! GCATTTTGAGCTTAAAGCAT+GGG + chr3_4:16415193-16415212 Msa0492580:CDS 35.0%
TCTGAATCTTCTGATGTTGT+TGG + chr3_4:16415224-16415243 Msa0492580:CDS 35.0%
TCATTTAATTCTTCGGCAGA+TGG + chr3_4:16415268-16415287 Msa0492580:CDS 35.0%
! CCCAATAAATTGGTGGTAAG+TGG - chr3_4:16414827-16414846 None:intergenic 40.0%
ATCTAGGCTCTATGAACAAC+TGG + chr3_4:16414880-16414899 Msa0492580:CDS 40.0%
GCAAAAGAAAGAGTTGGGAT+TGG + chr3_4:16414925-16414944 Msa0492580:CDS 40.0%
GCTCCTATTGCTAGTAATGA+AGG + chr3_4:16414953-16414972 Msa0492580:CDS 40.0%
GTGGATGAGTCAAGCATATA+TGG + chr3_4:16415021-16415040 Msa0492580:intron 40.0%
! CTATGCTGATTATTGGAACC+CGG - chr3_4:16415106-16415125 None:intergenic 40.0%
CATTGTACACAAGCTTAGCA+AGG - chr3_4:16415157-16415176 None:intergenic 40.0%
GATTGTCATCTACAGCTCTG+TGG + chr3_4:16415002-16415021 Msa0492580:intron 45.0%
TTTACTTGCAGGCATGACAG+TGG + chr3_4:16415077-16415096 Msa0492580:intron 45.0%
! ATCAGCATAGTCGGTTTAGG+AGG + chr3_4:16415116-16415135 Msa0492580:CDS 45.0%
! TCAGCATAGTCGGTTTAGGA+GGG + chr3_4:16415117-16415136 Msa0492580:CDS 45.0%
! ATAGTCGGTTTAGGAGGGAT+GGG + chr3_4:16415122-16415141 Msa0492580:CDS 45.0%
! CATAGTCGGTTTAGGAGGGA+TGG + chr3_4:16415121-16415140 Msa0492580:CDS 50.0%
! TAGTCGGTTTAGGAGGGATG+GGG + chr3_4:16415123-16415142 Msa0492580:CDS 50.0%
GTACACAAGCTTAGCAAGGG+TGG - chr3_4:16415153-16415172 None:intergenic 50.0%
GCAGGCATGACAGTGGCAAC+CGG + chr3_4:16415084-16415103 Msa0492580:intron 60.0%
CAGGCATGACAGTGGCAACC+GGG + chr3_4:16415085-16415104 Msa0492580:intron 60.0%
Chromosome Type Strat End Strand Name
chr3_4 gene 16414794 16415301 16414794 ID=Msa0492580;Name=Msa0492580
chr3_4 mRNA 16414794 16415301 16414794 ID=Msa0492580-mRNA-1;Parent=Msa0492580;Name=Msa0492580-mRNA-1;_AED=0.47;_eAED=0.47;_QI=0|0|0|1|1|1|2|0|141
chr3_4 exon 16414794 16415005 16414794 ID=Msa0492580-mRNA-1:exon:4725;Parent=Msa0492580-mRNA-1
chr3_4 exon 16415088 16415301 16415088 ID=Msa0492580-mRNA-1:exon:4726;Parent=Msa0492580-mRNA-1
chr3_4 CDS 16414794 16415005 16414794 ID=Msa0492580-mRNA-1:cds;Parent=Msa0492580-mRNA-1
chr3_4 CDS 16415088 16415301 16415088 ID=Msa0492580-mRNA-1:cds;Parent=Msa0492580-mRNA-1
Gene Sequence

>Msa0492580

ATGCTAAATAAGTTGAAAGCTGAATCTGAACCACTTACCACCAATTTATTGGGTTTGGTTTCAGCTTTAACTACAAATCCATTTGAATCTAGGCTCTATGAACAACTGGATAAACTAGAACTTCTTGCAAAGCAAAAGAAAGAGTTGGGATTGGGAGAAGCTCCTATTGCTAGTAATGAAGGCTTAGTCAGTTGCAAACCTTCAAAAAGATTGCATGACAGTGGCAACCGGGTTCCAATAATCAGCATAGTCGGTTTAGGAGGGATGGGGAAAACCACCCTTGCTAAGCTTGTGTACAATGACAACAAGATAGAGGAGCATTTTGAGCTTAAAGCATGGGTCTATGTTTCTGAATCTTCTGATGTTGTTGGACTCACCAAAGCAATTCTAAGTCATTTAATTCTTCGGCAGATGGTGAATACTTGA
Protein sequence

>Msa0492580

MLNKLKAESEPLTTNLLGLVSALTTNPFESRLYEQLDKLELLAKQKKELGLGEAPIASNEGLVSCKPSKRLHDSGNRVPIISIVGLGGMGKTTLAKLVYNDNKIEEHFELKAWVYVSESSDVVGLTKAILSHLILRQMVNT*