AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa0511430

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0511430 MtrunA17_Chr3g0117751 98.780 246 3 0 1 246 385 630 5.87e-178 503
Msa0511430 MtrunA17_Chr4g0049841 53.171 205 89 4 43 246 510 708 1.18e-64 213
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0511430 AT1G27510.1 68.699 246 74 3 2 246 408 651 9.14e-117 348
Msa0511430 AT4G33630.1 51.724 203 90 4 44 244 486 682 2.95e-59 199
Msa0511430 AT4G33630.2 51.724 203 90 4 44 244 486 682 2.95e-59 199
Msa0511430 AT4G33630.3 51.724 203 90 4 44 244 486 682 2.95e-59 199

Find 120 sgRNAs with CRISPR-Local

Find 229 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
CTGATGTTGCTAAGGCCTTT+TGG 0.172201 3_4:+60980647 Msa0511430:CDS
GTCCAATTGAGACGAAAATT+TGG 0.216706 3_4:+60981842 Msa0511430:CDS
GGGGAATTGCTTCAACTTAA+TGG 0.218721 3_4:+60982958 Msa0511430:CDS
AATTCCCCATCAACCCATTT+TGG 0.219963 3_4:-60982943 None:intergenic
GGGATCCTTTATGTGCATTA+TGG 0.255430 3_4:+60983478 Msa0511430:three_prime_UTR
GACAATTTCACGCATGCTTT+TGG 0.267932 3_4:-60981589 None:intergenic
GATTGTCCCCGATGTCAATT+TGG 0.283219 3_4:+60983893 Msa0511430:three_prime_UTR
CAATTTGGGTTTGGCTAGTT+TGG 0.285639 3_4:+60983908 Msa0511430:three_prime_UTR
GGACTCTATGTTGGTGCATT+TGG 0.288125 3_4:+60981800 Msa0511430:intron
CTTTATGTGCATTATGGTTA+TGG 0.295677 3_4:+60983484 Msa0511430:three_prime_UTR
GTGACATTCCGTGCTAAAAT+TGG 0.301622 3_4:+60982088 Msa0511430:CDS
GTTGGTGCATTTGGACCTTA+TGG 0.309966 3_4:+60981809 Msa0511430:CDS
GAACCTTCTTAAAACTCTTC+TGG 0.328947 3_4:+60983645 Msa0511430:three_prime_UTR
AAACAGAATGCAAGTTATAA+AGG 0.347493 3_4:+60982886 Msa0511430:intron
CCCCGATGTCAATTTGGGTT+TGG 0.352260 3_4:+60983899 Msa0511430:three_prime_UTR
CTGTGTGCCTTCTTGTTTGT+GGG 0.355176 3_4:+60983507 Msa0511430:three_prime_UTR
CGAAATCCAAAATGGGTTGA+TGG 0.365952 3_4:+60982937 Msa0511430:CDS
TGGACTGTATCCTGATGAAT+TGG 0.374202 3_4:+60982135 Msa0511430:CDS
TTCTTCTGTGTTTAGGGCAT+GGG 0.376637 3_4:+60983095 Msa0511430:intron
ATTATTTAGTCTTAAAAGAA+AGG 0.384034 3_4:+60983770 Msa0511430:three_prime_UTR
ATTGTCCCCGATGTCAATTT+GGG 0.397971 3_4:+60983894 Msa0511430:three_prime_UTR
GCAACAAAACGAAATTATAA+AGG 0.405712 3_4:-60983367 None:intergenic
CACATGAAAGGAGCAGATCT+TGG 0.407586 3_4:+60983122 Msa0511430:CDS
TTGAGTTGGCCTGGTGGTGT+TGG 0.413303 3_4:+60983829 Msa0511430:three_prime_UTR
ACATATGATAACATAGAGAT+TGG 0.417262 3_4:-60983398 None:intergenic
TTCTGATAAAGTCTCTTCTA+AGG 0.422012 3_4:+60980672 Msa0511430:CDS
CTTTCTTCCCTGACGTTGGT+GGG 0.425639 3_4:+60983233 Msa0511430:three_prime_UTR
TTTGATGCAGGCAGAATACC+TGG 0.427706 3_4:-60983535 None:intergenic
CATAACCATAATGCACATAA+AGG 0.433042 3_4:-60983483 None:intergenic
TTTGCTCGGGCACAGCATAA+AGG 0.436923 3_4:-60983148 None:intergenic
TACACCAACCCACCAACGTC+AGG 0.440026 3_4:-60983241 None:intergenic
CTTCCCTGACGTTGGTGGGT+TGG 0.443452 3_4:+60983237 Msa0511430:three_prime_UTR
AGGAGCTATAGAAAGACCTT+AGG 0.444366 3_4:+60983457 Msa0511430:three_prime_UTR
CCCCTCTGGGACTTGCAATC+CGG 0.451843 3_4:-60981671 None:intergenic
TTTACCACTCCCAATTCATC+AGG 0.451893 3_4:-60982145 None:intergenic
CCGGTTAAAATATGTATCTT+CGG 0.453570 3_4:-60981652 None:intergenic
TCTTTCTTCCCTGACGTTGG+TGG 0.456581 3_4:+60983232 Msa0511430:three_prime_UTR
TCTTAATGTTCCTGCAGGCC+AGG 0.458683 3_4:+60981949 Msa0511430:CDS
GCCAAACCCAAATTGACATC+GGG 0.460570 3_4:-60983900 None:intergenic
CAGGGAAGAAAGAAATTTGA+AGG 0.462451 3_4:-60983222 None:intergenic
TGGACTATTTCGATGCCATA+AGG 0.462771 3_4:-60981824 None:intergenic
GCTGTGTGCCTTCTTGTTTG+TGG 0.463974 3_4:+60983506 Msa0511430:three_prime_UTR
GCAAATGTTAGTCTGTTAGT+AGG 0.473639 3_4:+60983594 Msa0511430:three_prime_UTR
GTGGTGTTGGCTTAAGACCT+TGG 0.473768 3_4:+60983842 Msa0511430:three_prime_UTR
CAAGGGAGAATAGCAGACTT+TGG 0.474937 3_4:+60982910 Msa0511430:CDS
TTCTCCTAGGTATATGTAAG+AGG 0.477355 3_4:-60980537 None:intergenic
TGTTGTTAAATCACTTGAGT+TGG 0.479925 3_4:+60983815 Msa0511430:three_prime_UTR
CAATATCAAAGTGGCAGCAC+TGG 0.480476 3_4:+60980594 Msa0511430:CDS
TCTTGTTTGTGGGAAGTCCC+AGG 0.481587 3_4:+60983517 Msa0511430:three_prime_UTR
CTAGCGGCTTCCTTTGATGC+AGG 0.482036 3_4:-60983547 None:intergenic
GACTTTATCAGAACCCCAAA+AGG 0.484724 3_4:-60980662 None:intergenic
GAAATCCAAAATGGGTTGAT+GGG 0.485558 3_4:+60982938 Msa0511430:CDS
CTTACTCAGGTAGTTTCAAT+CGG 0.485754 3_4:-60983190 None:intergenic
TACTGACAAAATAGTGTGCC+AGG 0.485915 3_4:+60983437 Msa0511430:three_prime_UTR
TCCATGTTCTTTATTTCCGC+TGG 0.487068 3_4:-60980502 None:intergenic
AGGTATTCTGCCTGCATCAA+AGG 0.487215 3_4:+60983537 Msa0511430:three_prime_UTR
GAAAGGATCAAAATCCCCTC+TGG 0.488511 3_4:-60981685 None:intergenic
TGAGACGAAAATTTGGACAT+TGG 0.493193 3_4:+60981849 Msa0511430:CDS
TTAAATCACTTGAGTTGGCC+TGG 0.499949 3_4:+60983820 Msa0511430:three_prime_UTR
GCATAAACCACCTGGCCTGC+AGG 0.501097 3_4:-60981959 None:intergenic
TATGCCTTCTGATGTTGCTA+AGG 0.502960 3_4:+60980639 Msa0511430:CDS
GTTGGTGGGTTGGTGTATGA+AGG 0.507420 3_4:+60983247 Msa0511430:three_prime_UTR
TAAGGTCTTTCTATAGCTCC+TGG 0.509013 3_4:-60983455 None:intergenic
GGAAATAAAGAACATGGAAA+GGG 0.510999 3_4:+60980507 Msa0511430:exon
CAAAGTCTGCTATTCTCCCT+TGG 0.511863 3_4:-60982909 None:intergenic
TAGCTCGGTTGGTGCATTGA+GGG 0.518283 3_4:-60983671 None:intergenic
CGGAAATAAAGAACATGGAA+AGG 0.521393 3_4:+60980506 Msa0511430:exon
GGGGATCTTAATGTTCCTGC+AGG 0.523907 3_4:+60981944 Msa0511430:CDS
GTAGAGGCAGTAAAATTGAC+TGG 0.527358 3_4:+60981923 Msa0511430:CDS
CATATGATAACATAGAGATT+GGG 0.528542 3_4:-60983397 None:intergenic
ACTATCGCTCAATCAAGAAA+AGG 0.530223 3_4:-60983299 None:intergenic
TAGCACAAGAAAACTTTGCT+CGG 0.535045 3_4:-60983162 None:intergenic
GGAGCTATAGAAAGACCTTA+GGG 0.537298 3_4:+60983458 Msa0511430:three_prime_UTR
GGAGAAATCTCGATAATGAC+AGG 0.537424 3_4:+60980554 Msa0511430:CDS
ATTTCTTTCTTCCCTGACGT+TGG 0.549004 3_4:+60983229 Msa0511430:three_prime_UTR
AGCACAAGAAAACTTTGCTC+GGG 0.559857 3_4:-60983161 None:intergenic
AATAAAACTTTAATTACTAG+CGG 0.560665 3_4:-60983563 None:intergenic
ATTGCTTCAACTTAATGGCA+AGG 0.564516 3_4:+60982963 Msa0511430:CDS
AATGCAAGTTATAAAGGCCA+AGG 0.568569 3_4:+60982892 Msa0511430:CDS
GGTCTTAAGCCAACACCACC+AGG 0.569425 3_4:-60983838 None:intergenic
AAAGGATCAAAATCCCCTCT+GGG 0.570948 3_4:-60981684 None:intergenic
AGATCTGCTCCTTTCATGTG+TGG 0.571340 3_4:-60983119 None:intergenic
GGACTGTATCCTGATGAATT+GGG 0.571701 3_4:+60982136 Msa0511430:CDS
CTTTCCTCTTACATATACCT+AGG 0.573230 3_4:+60980533 Msa0511430:CDS
TCATTATCGAGATTTCTCCT+AGG 0.575590 3_4:-60980550 None:intergenic
ACCGGATTGCAAGTCCCAGA+GGG 0.577368 3_4:+60981670 Msa0511430:CDS
CTAGTCTCGCGTAGATAGCT+CGG 0.579515 3_4:-60983686 None:intergenic
AGGGAAGAAAGAAATTTGAA+GGG 0.586434 3_4:-60983221 None:intergenic
TAGAGGCAGTAAAATTGACT+GGG 0.586779 3_4:+60981924 Msa0511430:CDS
ATTTGGACATTGGAATGATG+TGG 0.588530 3_4:+60981859 Msa0511430:CDS
ATAGCTCGGTTGGTGCATTG+AGG 0.591650 3_4:-60983672 None:intergenic
TCCAGCGGAAATAAAGAACA+TGG 0.592636 3_4:+60980501 Msa0511430:exon
AGCCAAACCCAAATTGACAT+CGG 0.601837 3_4:-60983901 None:intergenic
AATAAAGAGTTGCATATCTG+TGG 0.606659 3_4:+60983726 Msa0511430:three_prime_UTR
GTCAACCGCAACGCCAACCG+TGG 0.607012 3_4:+60982115 Msa0511430:CDS
GGGACTTCCCACAAACAAGA+AGG 0.607023 3_4:-60983514 None:intergenic
GGCATGGGACCACACATGAA+AGG 0.609118 3_4:+60983110 Msa0511430:CDS
TAAAGTTCGCAATATCAAAG+TGG 0.611705 3_4:+60980585 Msa0511430:CDS
AAGGCCTTAGCAACATCAGA+AGG 0.612039 3_4:-60980643 None:intergenic
AAATCCAAAATGGGTTGATG+GGG 0.623944 3_4:+60982939 Msa0511430:CDS
ATCAGGATACAGTCCACGGT+TGG 0.625330 3_4:-60982128 None:intergenic
CTTGAGATGAGCAAACTCCA+AGG 0.625884 3_4:-60983859 None:intergenic
ACAGTCCACGGTTGGCGTTG+CGG 0.626828 3_4:-60982120 None:intergenic
TAAGCCAACTCACCGTCGAA+AGG 0.627500 3_4:-60981702 None:intergenic
GTGGCAGCACTGGCAGCACA+AGG 0.629400 3_4:+60980604 Msa0511430:CDS
ATGCACATAAAGGATCCCTA+AGG 0.630826 3_4:-60983473 None:intergenic
TCTCGCGTAGATAGCTCGGT+TGG 0.630897 3_4:-60983682 None:intergenic
TAAAATGCAGGACTCTATGT+TGG 0.634434 3_4:+60981791 Msa0511430:intron
GTATCCTGATGAATTGGGAG+TGG 0.636503 3_4:+60982141 Msa0511430:CDS
ACACCAACCCACCAACGTCA+GGG 0.648219 3_4:-60983240 None:intergenic
ATGCAAGTTATAAAGGCCAA+GGG 0.650434 3_4:+60982893 Msa0511430:CDS
TGAATTTATACGTCTTCCAG+CGG 0.652090 3_4:+60980486 None:intergenic
ATTCATCAGGATACAGTCCA+CGG 0.657204 3_4:-60982132 None:intergenic
TTGATGCAGGCAGAATACCT+GGG 0.663906 3_4:-60983534 None:intergenic
AACCGGATTGCAAGTCCCAG+AGG 0.666112 3_4:+60981669 Msa0511430:CDS
GAAATTATAAAGGCATCGTG+AGG 0.667719 3_4:-60983357 None:intergenic
AATCACTTGAGTTGGCCTGG+TGG 0.675244 3_4:+60983823 Msa0511430:three_prime_UTR
CCGGATTGCAAGTCCCAGAG+GGG 0.701896 3_4:+60981671 Msa0511430:CDS
CCAAACCCAAATTGACATCG+GGG 0.737688 3_4:-60983899 None:intergenic
AGAGGCAGTAAAATTGACTG+GGG 0.742487 3_4:+60981925 Msa0511430:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!!! TGATTATATCATTTAAAATT+TGG + chr3_4:60981734-60981753 Msa0511430:intron 10.0%
!!! GATTATATCATTTAAAATTT+GGG + chr3_4:60981735-60981754 Msa0511430:intron 10.0%
!!! AAAAGTATATAAATACAAAA+AGG - chr3_4:60982011-60982030 None:intergenic 10.0%
!!! AAAGCAATTTTTTTTTTTTA+TGG + chr3_4:60982058-60982077 Msa0511430:intron 10.0%
!!! TTTTAAAATGTATTTTATTG+AGG + chr3_4:60982745-60982764 Msa0511430:intron 10.0%
!! ATATTTATCTTAAATTGCTT+GGG + chr3_4:60981462-60981481 Msa0511430:intron 15.0%
!!! TTTTTTTTTTTATGGTGTAT+AGG + chr3_4:60982066-60982085 Msa0511430:intron 15.0%
!! AATAAAACTTTAATTACTAG+CGG - chr3_4:60983566-60983585 None:intergenic 15.0%
!!! ATTATTTAGTCTTAAAAGAA+AGG + chr3_4:60983770-60983789 Msa0511430:three_prime_UTR 15.0%
!! TAGTATATTATAAACACTAG+AGG + chr3_4:60980894-60980913 Msa0511430:intron 20.0%
!!! CAACTATTTTTATGTTATGT+TGG - chr3_4:60981370-60981389 None:intergenic 20.0%
!! TTAAATCTAAGCATTTATGT+TGG - chr3_4:60981403-60981422 None:intergenic 20.0%
!! CATATTTATCTTAAATTGCT+TGG + chr3_4:60981461-60981480 Msa0511430:intron 20.0%
!! TAAATGATATAATCAACACA+AGG - chr3_4:60981729-60981748 None:intergenic 20.0%
!! TCAAATAAATGTGAGAAAAT+TGG + chr3_4:60982364-60982383 Msa0511430:intron 20.0%
!!! ATTTTACTGATTTACATCTT+TGG + chr3_4:60982773-60982792 Msa0511430:intron 20.0%
! ATTATTATCATGATCGTAAC+TGG - chr3_4:60980805-60980824 None:intergenic 25.0%
!! ATTTTCATTTCTCCAAAATC+TGG + chr3_4:60981118-60981137 Msa0511430:intron 25.0%
!! TTTTCATTTCTCCAAAATCT+GGG + chr3_4:60981119-60981138 Msa0511430:intron 25.0%
!!! TTCTTACTTTCAAGTTTCAA+CGG - chr3_4:60981164-60981183 None:intergenic 25.0%
! ATAGCCAACTAAAAATTGAT+GGG - chr3_4:60981261-60981280 None:intergenic 25.0%
! AACATAATGTCATATTCTTG+AGG + chr3_4:60981427-60981446 Msa0511430:intron 25.0%
!!! AAGAAAAAGCAGTATTTTTG+AGG - chr3_4:60981530-60981549 None:intergenic 25.0%
! ATAACAGATTGTTTGAAAAC+AGG - chr3_4:60982228-60982247 None:intergenic 25.0%
!! CAAAACAACTTAAGCTTTTT+GGG + chr3_4:60982477-60982496 Msa0511430:intron 25.0%
!! AACTTTTCAAAAGAAGAATG+AGG - chr3_4:60982581-60982600 None:intergenic 25.0%
! GAATAATTACTATTCGAAAG+AGG - chr3_4:60982682-60982701 None:intergenic 25.0%
! ATGAAAACTCAAGAAAACTT+GGG - chr3_4:60982806-60982825 None:intergenic 25.0%
! AATGAAAACTCAAGAAAACT+TGG - chr3_4:60982807-60982826 None:intergenic 25.0%
!! GTTTTCTTGAGTTTTCATTT+TGG + chr3_4:60982808-60982827 Msa0511430:intron 25.0%
! AAACAGAATGCAAGTTATAA+AGG + chr3_4:60982886-60982905 Msa0511430:intron 25.0%
! ATTAATGTTCTTCTGTGTTT+AGG + chr3_4:60983088-60983107 Msa0511430:intron 25.0%
! TTAATGTTCTTCTGTGTTTA+GGG + chr3_4:60983089-60983108 Msa0511430:intron 25.0%
! GCAACAAAACGAAATTATAA+AGG - chr3_4:60983370-60983389 None:intergenic 25.0%
! CATATGATAACATAGAGATT+GGG - chr3_4:60983400-60983419 None:intergenic 25.0%
! ACATATGATAACATAGAGAT+TGG - chr3_4:60983401-60983420 None:intergenic 25.0%
GGAAATAAAGAACATGGAAA+GGG + chr3_4:60980507-60980526 Msa0511430:exon 30.0%
TAAAGTTCGCAATATCAAAG+TGG + chr3_4:60980585-60980604 Msa0511430:CDS 30.0%
TTCTGATAAAGTCTCTTCTA+AGG + chr3_4:60980672-60980691 Msa0511430:CDS 30.0%
TATTATAAACACTAGAGGTG+TGG + chr3_4:60980899-60980918 Msa0511430:intron 30.0%
ACGTATATTAGAGAAGAAAG+TGG + chr3_4:60980989-60981008 Msa0511430:intron 30.0%
TTTCATTTCTCCAAAATCTG+GGG + chr3_4:60981120-60981139 Msa0511430:intron 30.0%
TAGCCAACTAAAAATTGATG+GGG - chr3_4:60981260-60981279 None:intergenic 30.0%
CATAGCCAACTAAAAATTGA+TGG - chr3_4:60981262-60981281 None:intergenic 30.0%
CAATGTAGATTATGACCATA+TGG - chr3_4:60981292-60981311 None:intergenic 30.0%
!!! ATCTACTTTTTTTCTGTGCT+TGG - chr3_4:60981629-60981648 None:intergenic 30.0%
CCGGTTAAAATATGTATCTT+CGG - chr3_4:60981655-60981674 None:intergenic 30.0%
!!! CCGAAGATACATATTTTAAC+CGG + chr3_4:60981652-60981671 Msa0511430:CDS 30.0%
!! GCTTATAGAATGGATTTTTC+TGG + chr3_4:60981767-60981786 Msa0511430:intron 30.0%
!!! GATTTTTCTGGTTAAAATGC+AGG + chr3_4:60981779-60981798 Msa0511430:intron 30.0%
!!! CGAGTTTTTTGAATATGTAG+AGG + chr3_4:60981907-60981926 Msa0511430:CDS 30.0%
!! TGACTTTACCAATTTTAGCA+CGG - chr3_4:60982099-60982118 None:intergenic 30.0%
GTGGTAAATACATATTCACA+TGG + chr3_4:60982160-60982179 Msa0511430:intron 30.0%
! TTCTCACATTTATTTGAAGC+AGG - chr3_4:60982361-60982380 None:intergenic 30.0%
TTTGAAAAAAGCTATTCCAC+CGG + chr3_4:60982408-60982427 Msa0511430:intron 30.0%
TTATGTAAAGACAATGCATG+TGG + chr3_4:60982452-60982471 Msa0511430:intron 30.0%
! GCAAAACAACTTAAGCTTTT+TGG + chr3_4:60982476-60982495 Msa0511430:intron 30.0%
TTCACACTATTTGATCAAAG+AGG - chr3_4:60982543-60982562 None:intergenic 30.0%
! TTTTCAAAAGAAGAATGAGG+TGG - chr3_4:60982578-60982597 None:intergenic 30.0%
GTTAAATATTAGCACATGTC+AGG + chr3_4:60982598-60982617 Msa0511430:intron 30.0%
GAATGACTTCAGCATTAAAT+TGG - chr3_4:60982643-60982662 None:intergenic 30.0%
TTCAACTATGAGGAAATTGA+TGG - chr3_4:60982709-60982728 None:intergenic 30.0%
TTTCCTCATAGTTGAAATGT+TGG + chr3_4:60982713-60982732 Msa0511430:intron 30.0%
!! TTGGTTTTCGAAATCCAAAA+TGG + chr3_4:60982929-60982948 Msa0511430:CDS 30.0%
! TGGTTTTCGAAATCCAAAAT+GGG + chr3_4:60982930-60982949 Msa0511430:CDS 30.0%
TTAATGGCAAGGTAAATTAG+TGG + chr3_4:60982974-60982993 Msa0511430:intron 30.0%
! TCAATACCCAAGCATATTTT+TGG + chr3_4:60983018-60983037 Msa0511430:intron 30.0%
AGGGAAGAAAGAAATTTGAA+GGG - chr3_4:60983224-60983243 None:intergenic 30.0%
CATAACCATAATGCACATAA+AGG - chr3_4:60983486-60983505 None:intergenic 30.0%
CTTTATGTGCATTATGGTTA+TGG + chr3_4:60983484-60983503 Msa0511430:three_prime_UTR 30.0%
AATAAAGAGTTGCATATCTG+TGG + chr3_4:60983726-60983745 Msa0511430:three_prime_UTR 30.0%
TGTTGTTAAATCACTTGAGT+TGG + chr3_4:60983815-60983834 Msa0511430:three_prime_UTR 30.0%
CGGAAATAAAGAACATGGAA+AGG + chr3_4:60980506-60980525 Msa0511430:exon 35.0%
CTTTCCTCTTACATATACCT+AGG + chr3_4:60980533-60980552 Msa0511430:CDS 35.0%
TTCTCCTAGGTATATGTAAG+AGG - chr3_4:60980540-60980559 None:intergenic 35.0%
TCATTATCGAGATTTCTCCT+AGG - chr3_4:60980553-60980572 None:intergenic 35.0%
!! GAAAGCATTTGTCATTGGTA+AGG - chr3_4:60981088-60981107 None:intergenic 35.0%
!! TGTGTGAAAGCATTTGTCAT+TGG - chr3_4:60981093-60981112 None:intergenic 35.0%
TTCATTTCTCCAAAATCTGG+GGG + chr3_4:60981121-60981140 Msa0511430:intron 35.0%
TGGCTATGTACATATCCATA+TGG + chr3_4:60981274-60981293 Msa0511430:intron 35.0%
CTTGTCAATGAACTGACTAA+TGG + chr3_4:60981547-60981566 Msa0511430:intron 35.0%
TAAAATGCAGGACTCTATGT+TGG + chr3_4:60981791-60981810 Msa0511430:intron 35.0%
GTCCAATTGAGACGAAAATT+TGG + chr3_4:60981842-60981861 Msa0511430:CDS 35.0%
! GTCCAAATTTTCGTCTCAAT+TGG - chr3_4:60981847-60981866 None:intergenic 35.0%
TGAGACGAAAATTTGGACAT+TGG + chr3_4:60981849-60981868 Msa0511430:CDS 35.0%
ATTTGGACATTGGAATGATG+TGG + chr3_4:60981859-60981878 Msa0511430:CDS 35.0%
TAGAGGCAGTAAAATTGACT+GGG + chr3_4:60981924-60981943 Msa0511430:CDS 35.0%
! CTTTTATATGTGAGCATGAC+AGG + chr3_4:60982035-60982054 Msa0511430:intron 35.0%
GGAAAAAATAGTGCACACAT+AGG - chr3_4:60982321-60982340 None:intergenic 35.0%
! CAGGAAAAAAGTGCACAAAT+AGG - chr3_4:60982342-60982361 None:intergenic 35.0%
!! TTTTGGGTAGTTAGTTCAGT+CGG + chr3_4:60982493-60982512 Msa0511430:intron 35.0%
AAATATTAGCACATGTCAGG+CGG + chr3_4:60982601-60982620 Msa0511430:intron 35.0%
TACTATTCGAAAGAGGAATG+AGG - chr3_4:60982675-60982694 None:intergenic 35.0%
CAACCAACATTTCAACTATG+AGG - chr3_4:60982719-60982738 None:intergenic 35.0%
TGCATTCTGTTTGTATACGA+GGG - chr3_4:60982878-60982897 None:intergenic 35.0%
TTGCATTCTGTTTGTATACG+AGG - chr3_4:60982879-60982898 None:intergenic 35.0%
AATGCAAGTTATAAAGGCCA+AGG + chr3_4:60982892-60982911 Msa0511430:CDS 35.0%
ATGCAAGTTATAAAGGCCAA+GGG + chr3_4:60982893-60982912 Msa0511430:CDS 35.0%
!! GAAATCCAAAATGGGTTGAT+GGG + chr3_4:60982938-60982957 Msa0511430:CDS 35.0%
!! AAATCCAAAATGGGTTGATG+GGG + chr3_4:60982939-60982958 Msa0511430:CDS 35.0%
ATTGCTTCAACTTAATGGCA+AGG + chr3_4:60982963-60982982 Msa0511430:CDS 35.0%
GTGAGACCAAAAATATGCTT+GGG - chr3_4:60983027-60983046 None:intergenic 35.0%
AGTGAGACCAAAAATATGCT+TGG - chr3_4:60983028-60983047 None:intergenic 35.0%
TAGCACAAGAAAACTTTGCT+CGG - chr3_4:60983165-60983184 None:intergenic 35.0%
CTTACTCAGGTAGTTTCAAT+CGG - chr3_4:60983193-60983212 None:intergenic 35.0%
CAGGGAAGAAAGAAATTTGA+AGG - chr3_4:60983225-60983244 None:intergenic 35.0%
ACTATCGCTCAATCAAGAAA+AGG - chr3_4:60983302-60983321 None:intergenic 35.0%
!! GAAATTATAAAGGCATCGTG+AGG - chr3_4:60983360-60983379 None:intergenic 35.0%
! GCAAATGTTAGTCTGTTAGT+AGG + chr3_4:60983594-60983613 Msa0511430:three_prime_UTR 35.0%
! TAGTTTTCGTGGTTTGAACT+CGG + chr3_4:60983622-60983641 Msa0511430:three_prime_UTR 35.0%
GAACCTTCTTAAAACTCTTC+TGG + chr3_4:60983645-60983664 Msa0511430:three_prime_UTR 35.0%
!!! ACACCAGAAGAGTTTTAAGA+AGG - chr3_4:60983651-60983670 None:intergenic 35.0%
TCCAGCGGAAATAAAGAACA+TGG + chr3_4:60980501-60980520 Msa0511430:exon 40.0%
! TCCATGTTCTTTATTTCCGC+TGG - chr3_4:60980505-60980524 None:intergenic 40.0%
GGAGAAATCTCGATAATGAC+AGG + chr3_4:60980554-60980573 Msa0511430:CDS 40.0%
TATGCCTTCTGATGTTGCTA+AGG + chr3_4:60980639-60980658 Msa0511430:CDS 40.0%
! TGATGTTGCTAAGGCCTTTT+GGG + chr3_4:60980648-60980667 Msa0511430:CDS 40.0%
GACTTTATCAGAACCCCAAA+AGG - chr3_4:60980665-60980684 None:intergenic 40.0%
ATGTTGTTGCACATCAAGAG+AGG + chr3_4:60980925-60980944 Msa0511430:intron 40.0%
!!! CAGCCCCATCAATTTTTAGT+TGG + chr3_4:60981254-60981273 Msa0511430:intron 40.0%
TCATATTCTTGAGGTCTGTG+CGG + chr3_4:60981436-60981455 Msa0511430:intron 40.0%
TTGGATACCTAAGTCAAGTG+AGG - chr3_4:60981573-60981592 None:intergenic 40.0%
! GACAATTTCACGCATGCTTT+TGG - chr3_4:60981592-60981611 None:intergenic 40.0%
AAAGGATCAAAATCCCCTCT+GGG - chr3_4:60981687-60981706 None:intergenic 40.0%
TGGACTATTTCGATGCCATA+AGG - chr3_4:60981827-60981846 None:intergenic 40.0%
GTAGAGGCAGTAAAATTGAC+TGG + chr3_4:60981923-60981942 Msa0511430:CDS 40.0%
AGAGGCAGTAAAATTGACTG+GGG + chr3_4:60981925-60981944 Msa0511430:CDS 40.0%
! TTGAAAAGGCATAAACCACC+TGG - chr3_4:60981970-60981989 None:intergenic 40.0%
GTGACATTCCGTGCTAAAAT+TGG + chr3_4:60982088-60982107 Msa0511430:CDS 40.0%
ATTCATCAGGATACAGTCCA+CGG - chr3_4:60982135-60982154 None:intergenic 40.0%
TGGACTGTATCCTGATGAAT+TGG + chr3_4:60982135-60982154 Msa0511430:CDS 40.0%
GGACTGTATCCTGATGAATT+GGG + chr3_4:60982136-60982155 Msa0511430:CDS 40.0%
TTTACCACTCCCAATTCATC+AGG - chr3_4:60982148-60982167 None:intergenic 40.0%
TTTCCCTTACATCATCATGC+TGG + chr3_4:60982201-60982220 Msa0511430:intron 40.0%
AATAGTGCACACATAGGCAT+AGG - chr3_4:60982315-60982334 None:intergenic 40.0%
TTTGGGTAGTTAGTTCAGTC+GGG + chr3_4:60982494-60982513 Msa0511430:intron 40.0%
!! CGAAATCCAAAATGGGTTGA+TGG + chr3_4:60982937-60982956 Msa0511430:CDS 40.0%
! AATTCCCCATCAACCCATTT+TGG - chr3_4:60982946-60982965 None:intergenic 40.0%
GGGGAATTGCTTCAACTTAA+TGG + chr3_4:60982958-60982977 Msa0511430:CDS 40.0%
! TTCTTCTGTGTTTAGGGCAT+GGG + chr3_4:60983095-60983114 Msa0511430:intron 40.0%
AGCACAAGAAAACTTTGCTC+GGG - chr3_4:60983164-60983183 None:intergenic 40.0%
!! AAGGGTTTTGTAGCTTACTC+AGG - chr3_4:60983206-60983225 None:intergenic 40.0%
ATTTCTTTCTTCCCTGACGT+TGG + chr3_4:60983229-60983248 Msa0511430:three_prime_UTR 40.0%
TACTGACAAAATAGTGTGCC+AGG + chr3_4:60983437-60983456 Msa0511430:three_prime_UTR 40.0%
TAAGGTCTTTCTATAGCTCC+TGG - chr3_4:60983458-60983477 None:intergenic 40.0%
AGGAGCTATAGAAAGACCTT+AGG + chr3_4:60983457-60983476 Msa0511430:three_prime_UTR 40.0%
GGAGCTATAGAAAGACCTTA+GGG + chr3_4:60983458-60983477 Msa0511430:three_prime_UTR 40.0%
ATGCACATAAAGGATCCCTA+AGG - chr3_4:60983476-60983495 None:intergenic 40.0%
GGGATCCTTTATGTGCATTA+TGG + chr3_4:60983478-60983497 Msa0511430:three_prime_UTR 40.0%
!!! AGTAGGCATGTTAGTTTTCG+TGG + chr3_4:60983611-60983630 Msa0511430:three_prime_UTR 40.0%
! AGTTTTCGTGGTTTGAACTC+GGG + chr3_4:60983623-60983642 Msa0511430:three_prime_UTR 40.0%
!! TTAAATCACTTGAGTTGGCC+TGG + chr3_4:60983820-60983839 Msa0511430:three_prime_UTR 40.0%
ATTGTCCCCGATGTCAATTT+GGG + chr3_4:60983894-60983913 Msa0511430:three_prime_UTR 40.0%
AGCCAAACCCAAATTGACAT+CGG - chr3_4:60983904-60983923 None:intergenic 40.0%
! CAATTTGGGTTTGGCTAGTT+TGG + chr3_4:60983908-60983927 Msa0511430:three_prime_UTR 40.0%
CAATATCAAAGTGGCAGCAC+TGG + chr3_4:60980594-60980613 Msa0511430:CDS 45.0%
AAGGCCTTAGCAACATCAGA+AGG - chr3_4:60980646-60980665 None:intergenic 45.0%
! CTGATGTTGCTAAGGCCTTT+TGG + chr3_4:60980647-60980666 Msa0511430:CDS 45.0%
! GATGTTGCTAAGGCCTTTTG+GGG + chr3_4:60980649-60980668 Msa0511430:CDS 45.0%
! TACTTGACACCCCCAGATTT+TGG - chr3_4:60981133-60981152 None:intergenic 45.0%
TGGGATATGTAGTTTCCGTC+TGG + chr3_4:60981481-60981500 Msa0511430:intron 45.0%
ACAGCTGAATACCACGCATT+GGG - chr3_4:60981507-60981526 None:intergenic 45.0%
CTAATGGCCTCACTTGACTT+AGG + chr3_4:60981563-60981582 Msa0511430:intron 45.0%
GAAAGGATCAAAATCCCCTC+TGG - chr3_4:60981688-60981707 None:intergenic 45.0%
!! GGGGATTTTGATCCTTTCGA+CGG + chr3_4:60981690-60981709 Msa0511430:CDS 45.0%
GCAGAGCAGAGCTTATAGAA+TGG + chr3_4:60981757-60981776 Msa0511430:intron 45.0%
GGACTCTATGTTGGTGCATT+TGG + chr3_4:60981800-60981819 Msa0511430:intron 45.0%
GTTGGTGCATTTGGACCTTA+TGG + chr3_4:60981809-60981828 Msa0511430:CDS 45.0%
CGATGCTCGAGAGATTGAAA+AGG - chr3_4:60981984-60982003 None:intergenic 45.0%
GTATCCTGATGAATTGGGAG+TGG + chr3_4:60982141-60982160 Msa0511430:CDS 45.0%
! GGTCCAGCATGATGATGTAA+GGG - chr3_4:60982207-60982226 None:intergenic 45.0%
! AGGTCCAGCATGATGATGTA+AGG - chr3_4:60982208-60982227 None:intergenic 45.0%
! TCTCTAACAATACCGACTGC+CGG - chr3_4:60982430-60982449 None:intergenic 45.0%
CAAAGTCTGCTATTCTCCCT+TGG - chr3_4:60982912-60982931 None:intergenic 45.0%
CAAGGGAGAATAGCAGACTT+TGG + chr3_4:60982910-60982929 Msa0511430:CDS 45.0%
! GTTCTTCTGTGTTTAGGGCA+TGG + chr3_4:60983094-60983113 Msa0511430:intron 45.0%
AGATCTGCTCCTTTCATGTG+TGG - chr3_4:60983122-60983141 None:intergenic 45.0%
CACATGAAAGGAGCAGATCT+TGG + chr3_4:60983122-60983141 Msa0511430:CDS 45.0%
!! GATAGTTTTACTCGACACGC+AGG + chr3_4:60983316-60983335 Msa0511430:three_prime_UTR 45.0%
! CTGTGTGCCTTCTTGTTTGT+GGG + chr3_4:60983507-60983526 Msa0511430:three_prime_UTR 45.0%
TTGATGCAGGCAGAATACCT+GGG - chr3_4:60983537-60983556 None:intergenic 45.0%
TTTGATGCAGGCAGAATACC+TGG - chr3_4:60983538-60983557 None:intergenic 45.0%
AGGTATTCTGCCTGCATCAA+AGG + chr3_4:60983537-60983556 Msa0511430:three_prime_UTR 45.0%
CTTGAGATGAGCAAACTCCA+AGG - chr3_4:60983862-60983881 None:intergenic 45.0%
GATTGTCCCCGATGTCAATT+TGG + chr3_4:60983893-60983912 Msa0511430:three_prime_UTR 45.0%
CCAAACCCAAATTGACATCG+GGG - chr3_4:60983902-60983921 None:intergenic 45.0%
GCCAAACCCAAATTGACATC+GGG - chr3_4:60983903-60983922 None:intergenic 45.0%
!!! ATTAAAGTTTTATTTTTTAT+AGG + chr3_4:60983572-60983591 Msa0511430:three_prime_UTR 5.0%
!! ATCTGGGGGTGTCAAGTAAG+TGG + chr3_4:60981135-60981154 Msa0511430:intron 50.0%
GACAGCTGAATACCACGCAT+TGG - chr3_4:60981508-60981527 None:intergenic 50.0%
TGATCCTTTCGACGGTGAGT+TGG + chr3_4:60981698-60981717 Msa0511430:intron 50.0%
TAAGCCAACTCACCGTCGAA+AGG - chr3_4:60981705-60981724 None:intergenic 50.0%
GGGGATCTTAATGTTCCTGC+AGG + chr3_4:60981944-60981963 Msa0511430:CDS 50.0%
TCTTAATGTTCCTGCAGGCC+AGG + chr3_4:60981949-60981968 Msa0511430:CDS 50.0%
ATCAGGATACAGTCCACGGT+TGG - chr3_4:60982131-60982150 None:intergenic 50.0%
AAAGCTATTCCACCGGCAGT+CGG + chr3_4:60982415-60982434 Msa0511430:intron 50.0%
TTTGCTCGGGCACAGCATAA+AGG - chr3_4:60983151-60983170 None:intergenic 50.0%
TCTTTCTTCCCTGACGTTGG+TGG + chr3_4:60983232-60983251 Msa0511430:three_prime_UTR 50.0%
CTTTCTTCCCTGACGTTGGT+GGG + chr3_4:60983233-60983252 Msa0511430:three_prime_UTR 50.0%
!! GTTGGTGGGTTGGTGTATGA+AGG + chr3_4:60983247-60983266 Msa0511430:three_prime_UTR 50.0%
! GCTGTGTGCCTTCTTGTTTG+TGG + chr3_4:60983506-60983525 Msa0511430:three_prime_UTR 50.0%
GGGACTTCCCACAAACAAGA+AGG - chr3_4:60983517-60983536 None:intergenic 50.0%
! TCTTGTTTGTGGGAAGTCCC+AGG + chr3_4:60983517-60983536 Msa0511430:three_prime_UTR 50.0%
! TAGCTCGGTTGGTGCATTGA+GGG - chr3_4:60983674-60983693 None:intergenic 50.0%
! ATAGCTCGGTTGGTGCATTG+AGG - chr3_4:60983675-60983694 None:intergenic 50.0%
CTAGTCTCGCGTAGATAGCT+CGG - chr3_4:60983689-60983708 None:intergenic 50.0%
!! AATCACTTGAGTTGGCCTGG+TGG + chr3_4:60983823-60983842 Msa0511430:three_prime_UTR 50.0%
!! GTGGTGTTGGCTTAAGACCT+TGG + chr3_4:60983842-60983861 Msa0511430:three_prime_UTR 50.0%
! CCCCGATGTCAATTTGGGTT+TGG + chr3_4:60983899-60983918 Msa0511430:three_prime_UTR 50.0%
ATACCACGCATTGGGCCAGA+CGG - chr3_4:60981499-60981518 None:intergenic 55.0%
AACCGGATTGCAAGTCCCAG+AGG + chr3_4:60981669-60981688 Msa0511430:CDS 55.0%
ACCGGATTGCAAGTCCCAGA+GGG + chr3_4:60981670-60981689 Msa0511430:CDS 55.0%
TAATGTTCCTGCAGGCCAGG+TGG + chr3_4:60981952-60981971 Msa0511430:intron 55.0%
! CTAACAATACCGACTGCCGG+TGG - chr3_4:60982427-60982446 None:intergenic 55.0%
GGCATGGGACCACACATGAA+AGG + chr3_4:60983110-60983129 Msa0511430:CDS 55.0%
ACACCAACCCACCAACGTCA+GGG - chr3_4:60983243-60983262 None:intergenic 55.0%
TACACCAACCCACCAACGTC+AGG - chr3_4:60983244-60983263 None:intergenic 55.0%
CTAGCGGCTTCCTTTGATGC+AGG - chr3_4:60983550-60983569 None:intergenic 55.0%
TCTCGCGTAGATAGCTCGGT+TGG - chr3_4:60983685-60983704 None:intergenic 55.0%
!! TTGAGTTGGCCTGGTGGTGT+TGG + chr3_4:60983829-60983848 Msa0511430:three_prime_UTR 55.0%
GGTCTTAAGCCAACACCACC+AGG - chr3_4:60983841-60983860 None:intergenic 55.0%
TTTCCGTCTGGCCCAATGCG+TGG + chr3_4:60981493-60981512 Msa0511430:intron 60.0%
!! CCCCTCTGGGACTTGCAATC+CGG - chr3_4:60981674-60981693 None:intergenic 60.0%
CCGGATTGCAAGTCCCAGAG+GGG + chr3_4:60981671-60981690 Msa0511430:CDS 60.0%
GCATAAACCACCTGGCCTGC+AGG - chr3_4:60981962-60981981 None:intergenic 60.0%
ACAGTCCACGGTTGGCGTTG+CGG - chr3_4:60982123-60982142 None:intergenic 60.0%
! CTTCCCTGACGTTGGTGGGT+TGG + chr3_4:60983237-60983256 Msa0511430:three_prime_UTR 60.0%
!! GTGGCAGCACTGGCAGCACA+AGG + chr3_4:60980604-60980623 Msa0511430:CDS 65.0%
GTCAACCGCAACGCCAACCG+TGG + chr3_4:60982115-60982134 Msa0511430:CDS 65.0%
Chromosome Type Strat End Strand Name
chr3_4 gene 60980491 60983939 60980491 ID=Msa0511430;Name=Msa0511430
chr3_4 mRNA 60980491 60983939 60980491 ID=Msa0511430-mRNA-1;Parent=Msa0511430;Name=Msa0511430-mRNA-1;_AED=0.00;_eAED=0.00;_QI=29|1|1|1|1|1|6|728|246
chr3_4 exon 60980491 60980693 60980491 ID=Msa0511430-mRNA-1:exon:14706;Parent=Msa0511430-mRNA-1
chr3_4 exon 60981585 60981711 60981585 ID=Msa0511430-mRNA-1:exon:14707;Parent=Msa0511430-mRNA-1
chr3_4 exon 60981801 60981970 60981801 ID=Msa0511430-mRNA-1:exon:14708;Parent=Msa0511430-mRNA-1
chr3_4 exon 60982088 60982162 60982088 ID=Msa0511430-mRNA-1:exon:14709;Parent=Msa0511430-mRNA-1
chr3_4 exon 60982892 60982984 60982892 ID=Msa0511430-mRNA-1:exon:14710;Parent=Msa0511430-mRNA-1
chr3_4 exon 60983110 60983939 60983110 ID=Msa0511430-mRNA-1:exon:14711;Parent=Msa0511430-mRNA-1
chr3_4 five_prime_UTR 60980491 60980519 60980491 ID=Msa0511430-mRNA-1:five_prime_utr;Parent=Msa0511430-mRNA-1
chr3_4 CDS 60980520 60980693 60980520 ID=Msa0511430-mRNA-1:cds;Parent=Msa0511430-mRNA-1
chr3_4 CDS 60981585 60981711 60981585 ID=Msa0511430-mRNA-1:cds;Parent=Msa0511430-mRNA-1
chr3_4 CDS 60981801 60981970 60981801 ID=Msa0511430-mRNA-1:cds;Parent=Msa0511430-mRNA-1
chr3_4 CDS 60982088 60982162 60982088 ID=Msa0511430-mRNA-1:cds;Parent=Msa0511430-mRNA-1
chr3_4 CDS 60982892 60982984 60982892 ID=Msa0511430-mRNA-1:cds;Parent=Msa0511430-mRNA-1
chr3_4 CDS 60983110 60983211 60983110 ID=Msa0511430-mRNA-1:cds;Parent=Msa0511430-mRNA-1
chr3_4 three_prime_UTR 60983212 60983939 60983212 ID=Msa0511430-mRNA-1:three_prime_utr;Parent=Msa0511430-mRNA-1
Gene Sequence

>Msa0511430

ATGGAAAGGGAATCTTTCCTCTTACATATACCTAGGAGAAATCTCGATAATGACAGGAAAGAAGATAAAGTTCGCAATATCAAAGTGGCAGCACTGGCAGCACAAGGAATCTCAGAGCTTATGCCTTCTGATGTTGCTAAGGCCTTTTGGGGTTCTGATAAAGTCTCTTCTAAGGTATCCAAAAGCATGCGTGAAATTGTCAAACTTGCTATCAGCCAAGCACAGAAAAAAAGTAGATTATCCGAAGATACATATTTTAACCGGATTGCAAGTCCCAGAGGGGATTTTGATCCTTTCGACGGACTCTATGTTGGTGCATTTGGACCTTATGGCATCGAAATAGTCCAATTGAGACGAAAATTTGGACATTGGAATGATGTGGACAGTGAAAACAGCACTTCAGATATCGAGTTTTTTGAATATGTAGAGGCAGTAAAATTGACTGGGGATCTTAATGTTCCTGCAGGCCAGGTGACATTCCGTGCTAAAATTGGTAAAGTCAACCGCAACGCCAACCGTGGACTGTATCCTGATGAATTGGGAGTGAATGCAAGTTATAAAGGCCAAGGGAGAATAGCAGACTTTGGTTTTCGAAATCCAAAATGGGTTGATGGGGAATTGCTTCAACTTAATGGCAAGGGCATGGGACCACACATGAAAGGAGCAGATCTTGGTTTCCTTTATGCTGTGCCCGAGCAAAGTTTTCTTGTGCTATTCAACCGATTGAAACTACCTGAGTAA
Protein sequence

>Msa0511430

MERESFLLHIPRRNLDNDRKEDKVRNIKVAALAAQGISELMPSDVAKAFWGSDKVSSKVSKSMREIVKLAISQAQKKSRLSEDTYFNRIASPRGDFDPFDGLYVGAFGPYGIEIVQLRRKFGHWNDVDSENSTSDIEFFEYVEAVKLTGDLNVPAGQVTFRAKIGKVNRNANRGLYPDELGVNASYKGQGRIADFGFRNPKWVDGELLQLNGKGMGPHMKGADLGFLYAVPEQSFLVLFNRLKLPE*