AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa1466140


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa1466140 MtrunA17_Chr8g0383701 99.462 186 1 0 1 186 913 1098 1.04e-124 377
Msa1466140 MtrunA17_Chr1g0201731 84.239 184 29 0 1 184 914 1097 5.20e-99 308
Msa1466140 MtrunA17_Chr2g0296991 73.913 184 47 1 1 183 861 1044 4.95e-94 294
Msa1466140 MtrunA17_Chr3g0088821 74.054 185 46 2 1 183 894 1078 1.20e-93 294
Msa1466140 MtrunA17_Chr8g0352231 74.054 185 46 2 1 183 894 1078 1.46e-92 291
Msa1466140 MtrunA17_Chr4g0073711 71.739 184 51 1 1 183 855 1038 1.56e-92 290
Msa1466140 MtrunA17_Chr3g0137951 71.196 184 50 2 3 183 904 1087 3.94e-87 276
Msa1466140 MtrunA17_Chr2g0284211 68.421 171 54 0 1 171 794 964 6.02e-83 264
Msa1466140 MtrunA17_Chr8g0379241 62.903 186 66 2 1 183 806 991 1.96e-82 262
Msa1466140 MtrunA17_Chr7g0267731 65.574 183 61 2 3 183 804 986 4.01e-80 256
Msa1466140 MtrunA17_Chr1g0183381 60.870 184 70 2 1 183 722 904 1.76e-76 245
Msa1466140 MtrunA17_Chr3g0078671 59.043 188 72 2 1 183 862 1049 8.17e-74 239
Msa1466140 MtrunA17_Chr8g0364641 55.914 186 79 3 1 183 796 981 1.16e-70 230
Msa1466140 MtrunA17_Chr4g0039801 48.000 175 88 1 1 172 959 1133 1.13e-55 189
Msa1466140 MtrunA17_Chr5g0410641 49.375 160 78 1 11 167 948 1107 4.85e-54 184
Msa1466140 MtrunA17_Chr7g0269741 48.538 171 84 2 1 167 986 1156 7.56e-54 184
Msa1466140 MtrunA17_Chr1g0165411 45.455 176 93 1 1 173 920 1095 6.63e-52 178
Msa1466140 MtrunA17_Chr0c03g0490021 66.393 122 40 1 15 135 1 122 8.50e-52 162
Msa1466140 MtrunA17_Chr2g0297351 47.590 166 84 1 5 167 845 1010 2.60e-50 173
Msa1466140 MtrunA17_Chr1g0165421 45.833 168 88 1 3 167 950 1117 1.40e-49 171
Msa1466140 MtrunA17_Chr7g0252781 61.176 85 31 1 101 183 5 89 7.25e-31 107
Msa1466140 MtrunA17_Chr8g0355061 77.778 54 12 0 15 68 1 54 1.21e-25 93.6
Msa1466140 MtrunA17_Chr7g0274751 35.443 158 45 3 19 173 701 804 4.94e-22 92.8
Msa1466140 MtrunA17_Chr2g0322331 27.607 163 107 4 2 155 208 368 1.24e-12 65.1
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa1466140 AT2G21770.1 91.160 181 16 0 3 183 906 1086 1.69e-112 345
Msa1466140 AT5G64740.1 89.617 183 19 0 1 183 901 1083 5.61e-112 343
Msa1466140 AT5G09870.1 87.978 183 22 0 1 183 886 1068 1.13e-110 339
Msa1466140 AT4G39350.1 89.071 183 20 0 1 183 900 1082 6.70e-104 322
Msa1466140 AT5G05170.1 74.054 185 46 2 1 183 881 1065 3.80e-93 293
Msa1466140 AT5G17420.1 70.109 184 54 1 1 183 843 1026 1.85e-90 285
Msa1466140 AT5G44030.3 70.430 186 52 2 1 183 585 770 1.50e-89 278
Msa1466140 AT5G44030.1 70.430 186 52 2 1 183 864 1049 8.93e-88 278
Msa1466140 AT5G44030.2 70.430 186 52 2 1 183 893 1078 9.30e-88 278
Msa1466140 AT4G32410.1 76.190 168 39 1 3 169 898 1065 1.19e-87 278
Msa1466140 AT2G25540.1 71.839 174 48 1 1 173 876 1049 5.04e-84 268
Msa1466140 AT4G18780.1 69.591 171 52 0 1 171 800 970 1.14e-83 266
Msa1466140 AT1G02730.1 49.123 171 83 2 1 167 998 1168 2.29e-54 186
Msa1466140 AT3G03050.1 50.000 166 80 1 5 167 966 1131 7.67e-54 184
Msa1466140 AT5G16910.1 50.602 166 79 1 5 167 966 1131 7.97e-54 184
Msa1466140 AT2G33100.2 46.471 170 88 1 1 167 855 1024 1.96e-52 180
Msa1466140 AT2G33100.1 46.471 170 88 1 1 167 855 1024 1.96e-52 180
Msa1466140 AT1G32180.1 46.707 167 84 1 5 166 801 967 1.36e-51 177
Msa1466140 AT1G32180.2 46.707 167 84 1 5 166 801 967 1.36e-51 177
Msa1466140 AT4G38190.1 45.882 170 87 3 3 167 923 1092 3.27e-49 171

Find 58 sgRNAs with CRISPR-Local

Find 66 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
CAAGGGTTTGCTTGGGAAAC+AGG 0.292557 tig0046052:-2208 Msa1466140:CDS
TAGATTATTCTTTGCCCTTT+GGG 0.328780 tig0046052:-2256 Msa1466140:CDS
GTAGATTATTCTTTGCCCTT+TGG 0.329014 tig0046052:-2257 Msa1466140:CDS
TAGGATGCCAACCATTGTTT+TGG 0.332128 tig0046052:-2184 Msa1466140:CDS
TTCATGATTAATAGCGTCAT+TGG 0.364253 tig0046052:+2351 Msa1466140:intergenic
TTCCATCTTGACTCTCTTGT+GGG 0.368361 tig0046052:-2142 Msa1466140:CDS
GTCTCCGATGCGATCAATAA+TGG 0.388015 tig0046052:-2306 Msa1466140:CDS
GGTGGAGGAATGAACAGTTT+TGG 0.392061 tig0046052:-2533 Msa1466140:CDS
CCATTCCTCAAGGGTTTGCT+TGG 0.392737 tig0046052:-2216 Msa1466140:CDS
GGTCCTGTCTTAGAAATTTG+TGG 0.442478 tig0046052:-2090 Msa1466140:CDS
TTCATATCCATCGCAGCAAC+CGG 0.452409 tig0046052:-2597 Msa1466140:CDS
CTTCCATCTTGACTCTCTTG+TGG 0.452869 tig0046052:-2143 Msa1466140:CDS
GGACCATCTCTTGATACAAA+AGG 0.456931 tig0046052:+2108 Msa1466140:intergenic
GACTCATGGGGACCTCTGTT+CGG 0.457318 tig0046052:-2279 Msa1466140:CDS
AGCCGACGATGGAGAATTCT+CGG 0.462265 tig0046052:-2412 Msa1466140:CDS
CATTCCTCAAGGGTTTGCTT+GGG 0.462332 tig0046052:-2215 Msa1466140:CDS
CCTTGAGCAAACCTTGGAAA+AGG 0.462786 tig0046052:+2478 Msa1466140:intergenic
CGGTATCCTTGAGATGCAAT+GGG 0.463735 tig0046052:-2577 Msa1466140:CDS
TCAATAATGGTTATGACTCA+TGG 0.469642 tig0046052:-2293 Msa1466140:CDS
CTATTAATCATGAATATTGT+GGG 0.471704 tig0046052:-2342 Msa1466140:CDS
AAGGATACCGGTTGCTGCGA+TGG 0.480309 tig0046052:+2590 Msa1466140:intergenic
CGGAGCTCTACGTATTCAAA+TGG 0.486111 tig0046052:-2392 Msa1466140:CDS
CCAAGCAAACCCTTGAGGAA+TGG 0.489223 tig0046052:+2216 Msa1466140:intergenic
AATATTGTGGGCGTGATTGT+TGG 0.489852 tig0046052:-2330 Msa1466140:CDS
AATCCACAAATTTCTAAGAC+AGG 0.496939 tig0046052:+2087 Msa1466140:intergenic
CAATAATGGTTATGACTCAT+GGG 0.497429 tig0046052:-2292 Msa1466140:CDS
CCGGTATCCTTGAGATGCAA+TGG 0.505381 tig0046052:-2578 Msa1466140:CDS
GCTATTAATCATGAATATTG+TGG 0.515248 tig0046052:-2343 Msa1466140:CDS
CTTGAGCAAACCTTGGAAAA+GGG 0.523859 tig0046052:+2479 Msa1466140:intergenic
GACCATCTCTTGATACAAAA+GGG 0.524535 tig0046052:+2109 Msa1466140:intergenic
GACCAAACCAAAACAATGGT+TGG 0.534995 tig0046052:+2177 Msa1466140:intergenic
CTAAAACCTTGAGCAAACCT+TGG 0.535272 tig0046052:+2472 Msa1466140:intergenic
GTTTGCTTGGGAAACAGGAT+AGG 0.535771 tig0046052:-2203 Msa1466140:CDS
AACCCACAAGAGAGTCAAGA+TGG 0.539781 tig0046052:+2140 Msa1466140:intergenic
ACATCTTTATCCATTCCTCA+AGG 0.543282 tig0046052:-2226 Msa1466140:CDS
GTGGTGTTGGAATAGATGAT+TGG 0.544543 tig0046052:-2554 Msa1466140:CDS
GTGTTGGAATAGATGATTGG+TGG 0.553168 tig0046052:-2551 Msa1466140:CDS
ATGATTAATAGCGTCATTGG+AGG 0.556639 tig0046052:+2354 Msa1466140:intergenic
CCATTGCATCTCAAGGATAC+CGG 0.562719 tig0046052:+2578 Msa1466140:intergenic
CATCTTTATCCATTCCTCAA+GGG 0.565197 tig0046052:-2225 Msa1466140:CDS
GAGATGCAATGGGGTGGTGT+TGG 0.571822 tig0046052:-2567 Msa1466140:CDS
CACCACCCCATTGCATCTCA+AGG 0.580716 tig0046052:+2571 Msa1466140:intergenic
AAGATGTATGATAACCCAAA+GGG 0.587645 tig0046052:+2242 Msa1466140:intergenic
TGATTAATAGCGTCATTGGA+GGG 0.597684 tig0046052:+2355 Msa1466140:intergenic
AAAGATGTATGATAACCCAA+AGG 0.604227 tig0046052:+2241 Msa1466140:intergenic
TTGGAATAGATGATTGGTGG+AGG 0.607703 tig0046052:-2548 Msa1466140:CDS
CTCCGAGAATTCTCCATCGT+CGG 0.612639 tig0046052:+2410 Msa1466140:intergenic
TTGCTGCGATGGATATGAAG+AGG 0.618185 tig0046052:+2601 Msa1466140:intergenic
ATAACCATTATTGATCGCAT+CGG 0.620303 tig0046052:+2302 Msa1466140:intergenic
GTTTCCCAAGCAAACCCTTG+AGG 0.630234 tig0046052:+2211 Msa1466140:intergenic
TGCTGCGATGGATATGAAGA+GGG 0.635078 tig0046052:+2602 Msa1466140:intergenic
ACATCAAAAGCAGCCGACGA+TGG 0.641221 tig0046052:-2423 Msa1466140:CDS
AAGAATAATCTACCGAACAG+AGG 0.650500 tig0046052:+2267 Msa1466140:intergenic
GATTGACCAAACCAAAACAA+TGG 0.654450 tig0046052:+2173 Msa1466140:intergenic
ATCCTTGAGATGCAATGGGG+TGG 0.682503 tig0046052:-2573 Msa1466140:CDS
AATAATGGTTATGACTCATG+GGG 0.693873 tig0046052:-2291 Msa1466140:CDS
AATTTGTGGATTGAATTGTG+AGG 0.730949 tig0046052:-2076 Msa1466140:CDS
GGTATCCTTGAGATGCAATG+GGG 0.741858 tig0046052:-2576 Msa1466140:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! CTATTAATCATGAATATTGT+GGG - tig0046052:2332-2351 Msa1466140:CDS 20.0%
! GCTATTAATCATGAATATTG+TGG - tig0046052:2331-2350 Msa1466140:CDS 25.0%
TTCATGATTAATAGCGTCAT+TGG + tig0046052:2326-2345 Msa1466140:intergenic 30.0%
ATAACCATTATTGATCGCAT+CGG + tig0046052:2375-2394 Msa1466140:intergenic 30.0%
TCAATAATGGTTATGACTCA+TGG - tig0046052:2381-2400 Msa1466140:CDS 30.0%
CAATAATGGTTATGACTCAT+GGG - tig0046052:2382-2401 Msa1466140:CDS 30.0%
AATAATGGTTATGACTCATG+GGG - tig0046052:2383-2402 Msa1466140:CDS 30.0%
TAGATTATTCTTTGCCCTTT+GGG - tig0046052:2418-2437 Msa1466140:CDS 30.0%
AAGATGTATGATAACCCAAA+GGG + tig0046052:2435-2454 Msa1466140:intergenic 30.0%
AAAGATGTATGATAACCCAA+AGG + tig0046052:2436-2455 Msa1466140:intergenic 30.0%
!! AATCCACAAATTTCTAAGAC+AGG + tig0046052:2590-2609 Msa1466140:intergenic 30.0%
! AATTTGTGGATTGAATTGTG+AGG - tig0046052:2598-2617 Msa1466140:CDS 30.0%
!!! AATGAACAGTTTTGGGTGAT+TGG - tig0046052:2149-2168 Msa1466140:CDS 35.0%
!!! CATTTTTTTGCCCTTTTCCA+AGG - tig0046052:2185-2204 Msa1466140:CDS 35.0%
TGATTAATAGCGTCATTGGA+GGG + tig0046052:2322-2341 Msa1466140:intergenic 35.0%
ATGATTAATAGCGTCATTGG+AGG + tig0046052:2323-2342 Msa1466140:intergenic 35.0%
AAGAATAATCTACCGAACAG+AGG + tig0046052:2410-2429 Msa1466140:intergenic 35.0%
GTAGATTATTCTTTGCCCTT+TGG - tig0046052:2417-2436 Msa1466140:CDS 35.0%
ACATCTTTATCCATTCCTCA+AGG - tig0046052:2448-2467 Msa1466140:CDS 35.0%
CATCTTTATCCATTCCTCAA+GGG - tig0046052:2449-2468 Msa1466140:CDS 35.0%
GATTGACCAAACCAAAACAA+TGG + tig0046052:2504-2523 Msa1466140:intergenic 35.0%
! AACCCTTTTGTATCAAGAGA+TGG - tig0046052:2563-2582 Msa1466140:CDS 35.0%
GACCATCTCTTGATACAAAA+GGG + tig0046052:2568-2587 Msa1466140:intergenic 35.0%
! GTGGTGTTGGAATAGATGAT+TGG - tig0046052:2120-2139 Msa1466140:CDS 40.0%
GTGTTGGAATAGATGATTGG+TGG - tig0046052:2123-2142 Msa1466140:CDS 40.0%
TTGGAATAGATGATTGGTGG+AGG - tig0046052:2126-2145 Msa1466140:CDS 40.0%
! GTGGAGGAATGAACAGTTTT+GGG - tig0046052:2142-2161 Msa1466140:CDS 40.0%
CTTGAGCAAACCTTGGAAAA+GGG + tig0046052:2198-2217 Msa1466140:intergenic 40.0%
CTAAAACCTTGAGCAAACCT+TGG + tig0046052:2205-2224 Msa1466140:intergenic 40.0%
AATATTGTGGGCGTGATTGT+TGG - tig0046052:2344-2363 Msa1466140:CDS 40.0%
! TAGGATGCCAACCATTGTTT+TGG - tig0046052:2490-2509 Msa1466140:CDS 40.0%
!!! TGCCAACCATTGTTTTGGTT+TGG - tig0046052:2495-2514 Msa1466140:CDS 40.0%
GACCAAACCAAAACAATGGT+TGG + tig0046052:2500-2519 Msa1466140:intergenic 40.0%
TTCCATCTTGACTCTCTTGT+GGG - tig0046052:2532-2551 Msa1466140:CDS 40.0%
GGACCATCTCTTGATACAAA+AGG + tig0046052:2569-2588 Msa1466140:intergenic 40.0%
GGTCCTGTCTTAGAAATTTG+TGG - tig0046052:2584-2603 Msa1466140:CDS 40.0%
TGCTGCGATGGATATGAAGA+GGG + tig0046052:2075-2094 None:intergenic 45.0%
TTGCTGCGATGGATATGAAG+AGG + tig0046052:2076-2095 None:intergenic 45.0%
TTCATATCCATCGCAGCAAC+CGG - tig0046052:2077-2096 Msa1466140:CDS 45.0%
CCATTGCATCTCAAGGATAC+CGG + tig0046052:2099-2118 Msa1466140:intergenic 45.0%
CGGTATCCTTGAGATGCAAT+GGG - tig0046052:2097-2116 Msa1466140:CDS 45.0%
GGTATCCTTGAGATGCAATG+GGG - tig0046052:2098-2117 Msa1466140:CDS 45.0%
! GGTGGAGGAATGAACAGTTT+TGG - tig0046052:2141-2160 Msa1466140:CDS 45.0%
!!! GAACAGTTTTGGGTGATTGG+AGG - tig0046052:2152-2171 Msa1466140:CDS 45.0%
CCTTGAGCAAACCTTGGAAA+AGG + tig0046052:2199-2218 Msa1466140:intergenic 45.0%
!! CCTTTTCCAAGGTTTGCTCA+AGG - tig0046052:2196-2215 Msa1466140:CDS 45.0%
!! GGTTTGCTCAAGGTTTTAGC+TGG - tig0046052:2206-2225 Msa1466140:CDS 45.0%
CGGAGCTCTACGTATTCAAA+TGG - tig0046052:2282-2301 Msa1466140:CDS 45.0%
GTCTCCGATGCGATCAATAA+TGG - tig0046052:2368-2387 Msa1466140:CDS 45.0%
! CATTCCTCAAGGGTTTGCTT+GGG - tig0046052:2459-2478 Msa1466140:CDS 45.0%
GTTTGCTTGGGAAACAGGAT+AGG - tig0046052:2471-2490 Msa1466140:CDS 45.0%
CTTCCATCTTGACTCTCTTG+TGG - tig0046052:2531-2550 Msa1466140:CDS 45.0%
AACCCACAAGAGAGTCAAGA+TGG + tig0046052:2537-2556 Msa1466140:intergenic 45.0%
CCGGTATCCTTGAGATGCAA+TGG - tig0046052:2096-2115 Msa1466140:CDS 50.0%
ATCCTTGAGATGCAATGGGG+TGG - tig0046052:2101-2120 Msa1466140:CDS 50.0%
ACATCAAAAGCAGCCGACGA+TGG - tig0046052:2251-2270 Msa1466140:CDS 50.0%
AGCCGACGATGGAGAATTCT+CGG - tig0046052:2262-2281 Msa1466140:CDS 50.0%
CTCCGAGAATTCTCCATCGT+CGG + tig0046052:2267-2286 Msa1466140:intergenic 50.0%
CCAAGCAAACCCTTGAGGAA+TGG + tig0046052:2461-2480 Msa1466140:intergenic 50.0%
! CCATTCCTCAAGGGTTTGCT+TGG - tig0046052:2458-2477 Msa1466140:CDS 50.0%
GTTTCCCAAGCAAACCCTTG+AGG + tig0046052:2466-2485 Msa1466140:intergenic 50.0%
! CAAGGGTTTGCTTGGGAAAC+AGG - tig0046052:2466-2485 Msa1466140:CDS 50.0%
! AAGGATACCGGTTGCTGCGA+TGG + tig0046052:2087-2106 Msa1466140:intergenic 55.0%
CACCACCCCATTGCATCTCA+AGG + tig0046052:2106-2125 Msa1466140:intergenic 55.0%
GAGATGCAATGGGGTGGTGT+TGG - tig0046052:2107-2126 Msa1466140:CDS 55.0%
GACTCATGGGGACCTCTGTT+CGG - tig0046052:2395-2414 Msa1466140:CDS 55.0%
Chromosome Type Strat End Strand Name
tig0046052 gene 2068 2628 2068 ID=Msa1466140;Name=Msa1466140
tig0046052 mRNA 2068 2628 2068 ID=Msa1466140-mRNA-1;Parent=Msa1466140;Name=Msa1466140-mRNA-1;_AED=0.28;_eAED=0.28;_QI=0|-1|0|1|-1|1|1|0|186
tig0046052 exon 2068 2628 2068 ID=Msa1466140-mRNA-1:exon:25000;Parent=Msa1466140-mRNA-1
tig0046052 CDS 2068 2628 2068 ID=Msa1466140-mRNA-1:cds;Parent=Msa1466140-mRNA-1
Gene Sequence

>Msa1466140

ATGGCCCTCTTCATATCCATCGCAGCAACCGGTATCCTTGAGATGCAATGGGGTGGTGTTGGAATAGATGATTGGTGGAGGAATGAACAGTTTTGGGTGATTGGAGGTGCTTCATCACATTTTTTTGCCCTTTTCCAAGGTTTGCTCAAGGTTTTAGCTGGTGTCGACACAAACTTCACTGTTACATCAAAAGCAGCCGACGATGGAGAATTCTCGGAGCTCTACGTATTCAAATGGACTTCGCTATTAATCCCTCCAATGACGCTATTAATCATGAATATTGTGGGCGTGATTGTTGGTGTCTCCGATGCGATCAATAATGGTTATGACTCATGGGGACCTCTGTTCGGTAGATTATTCTTTGCCCTTTGGGTTATCATACATCTTTATCCATTCCTCAAGGGTTTGCTTGGGAAACAGGATAGGATGCCAACCATTGTTTTGGTTTGGTCAATCTTGCTAGCTTCCATCTTGACTCTCTTGTGGGTTAGAGTTAACCCTTTTGTATCAAGAGATGGTCCTGTCTTAGAAATTTGTGGATTGAATTGTGAGGATACATGA

Protein sequence

>Msa1466140

MALFISIAATGILEMQWGGVGIDDWWRNEQFWVIGGASSHFFALFQGLLKVLAGVDTNFTVTSKAADDGEFSELYVFKWTSLLIPPMTLLIMNIVGVIVGVSDAINNGYDSWGPLFGRLFFALWVIIHLYPFLKGLLGKQDRMPTIVLVWSILLASILTLLWVRVNPFVSRDGPVLEICGLNCEDT*