AlfalfaGEDB | Msa0527680

M. sativa cultivar zhongmu-4 / Msa0527680

KEGG: K02183
GO: GO:0003674 GO:0005488 GO:0005509 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005739 GO:0043167 GO:0043169 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0044424 GO:0044444 GO:0044464 GO:0046872
PFAM: PF13499.9

Nr:

Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa0527680	RHN61981.1	88.514	148	17	0	5	152	33	180	4.46e-85	257

Swissprot:

Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa0527680	sp\|Q93Z27\|CML46_ARATH	41.593	113	63	2	42	151	91	203	1.13e-21	89.4

Trembl:

Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa0527680	A0A396IB26	88.514	148	17	0	5	152	33	180	2.13e-85	257

TFs/TRs:

Gene ID	Type	Classification

Protein Kinases:

Gene ID	Type	Classification

Network:

Co-expression Network

Gene1	Gene2	correlation coefficient	p_value	FDR
Msa0054310	Msa0527680	0.805642	1.209805e-49	-8.615850e-47
Msa0148720	Msa0527680	0.812740	3.641880e-51	-8.615850e-47
Msa0173990	Msa0527680	0.808017	3.807405e-50	-8.615850e-47
Msa0456200	Msa0527680	0.800633	1.315315e-48	-8.615850e-47
Msa0317510	Msa0527680	0.809308	2.017546e-50	-8.615850e-47
Msa0353690	Msa0527680	0.814571	1.439738e-51	-8.615850e-47
Msa0527680	Msa0865390	0.801113	1.049508e-48	-8.615850e-47
Msa0527680	Msa0902020	0.821129	4.758785e-53	-8.615850e-47
Msa0527680	Msa1082500	0.807923	3.986744e-50	-8.615850e-47
Msa0527680	Msa1172620	0.901424	2.717949e-78	-8.615850e-47
Msa0527680	Msa1384370	0.987069	6.012173e-169	-8.615850e-47

PPI

Gene1	Gene2	Type

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa0527680	MtrunA17_Chr4g0042491	88.514	148	17	0	5	152	33	180	4.10e-89	257
Msa0527680	MtrunA17_Chr5g0408251	43.333	150	77	4	6	148	48	196	3.50e-24	92.8
Msa0527680	MtrunA17_Chr7g0217621	45.968	124	63	3	28	148	76	198	7.07e-23	89.7
Msa0527680	MtrunA17_Chr4g0071941	47.009	117	59	2	38	151	33	149	2.57e-22	86.7
Msa0527680	MtrunA17_Chr4g0033601	35.200	125	62	4	41	148	20	142	5.43e-15	67.8
Msa0527680	MtrunA17_Chr7g0245911	51.515	66	31	1	85	149	127	192	7.26e-14	65.9
Msa0527680	MtrunA17_Chr1g0161211	50.000	70	34	1	84	152	117	186	1.92e-13	64.7
Msa0527680	MtrunA17_Chr2g0329371	52.459	61	27	1	90	148	143	203	2.50e-13	64.7
Msa0527680	MtrunA17_Chr4g0044961	32.787	122	69	2	41	150	72	192	8.93e-13	62.8
Msa0527680	MtrunA17_Chr5g0440931	44.595	74	41	0	74	147	73	146	1.86e-12	61.2
Msa0527680	MtrunA17_Chr6g0460211	44.595	74	41	0	74	147	73	146	1.98e-12	61.2
Msa0527680	MtrunA17_Chr7g0254731	44.595	74	41	0	74	147	73	146	2.02e-12	60.8
Msa0527680	MtrunA17_Chr3g0093271	44.595	74	41	0	74	147	124	197	4.75e-12	60.8
Msa0527680	MtrunA17_Chr7g0256701	49.231	65	32	1	85	148	149	213	5.23e-12	61.2
Msa0527680	MtrunA17_Chr3g0139311	50.769	65	30	1	85	147	114	178	6.59e-12	60.5
Msa0527680	MtrunA17_Chr1g0188171	40.541	74	44	0	74	147	73	146	1.03e-11	59.3
Msa0527680	MtrunA17_Chr3g0111371	34.821	112	66	2	39	150	34	138	1.12e-11	58.9
Msa0527680	MtrunA17_Chr5g0402361	43.529	85	45	1	66	147	97	181	1.72e-11	60.8
Msa0527680	MtrunA17_Chr5g0402361	43.023	86	44	2	66	147	270	354	9.28e-11	58.5
Msa0527680	MtrunA17_Chr8g0392951	36.607	112	61	3	39	147	53	157	2.18e-11	58.5
Msa0527680	MtrunA17_Chr5g0402331	43.023	86	44	2	66	147	98	182	3.04e-11	58.5
Msa0527680	MtrunA17_Chr3g0116141	41.250	80	45	1	71	148	60	139	3.70e-11	57.4
Msa0527680	MtrunA17_Chr7g0256171	36.607	112	60	4	42	148	37	142	4.00e-11	57.8
Msa0527680	MtrunA17_Chr3g0116151	38.710	93	50	2	63	148	48	140	4.14e-11	57.4

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa0527680	AT5G39670.1	41.593	113	63	2	42	151	91	203	1.15e-22	89.4
Msa0527680	AT3G29000.1	46.429	112	55	4	43	151	84	193	2.51e-20	83.2
Msa0527680	AT3G47480.1	53.623	69	31	1	85	152	115	183	2.05e-15	70.1
Msa0527680	AT1G05990.1	44.330	97	48	3	57	148	48	143	3.76e-14	65.9
Msa0527680	AT4G03290.1	44.444	99	49	4	57	149	48	146	9.92e-14	65.1
Msa0527680	AT3G07490.1	49.180	61	29	1	90	148	82	142	4.30e-13	63.2
Msa0527680	AT1G66410.4	44.595	74	41	0	74	147	37	110	6.14e-13	62.0
Msa0527680	AT1G66410.3	44.595	74	41	0	74	147	37	110	6.14e-13	62.0
Msa0527680	AT5G44460.1	47.887	71	35	1	80	148	105	175	7.18e-13	63.2
Msa0527680	AT1G66410.1	44.595	74	41	0	74	147	73	146	1.15e-12	62.0
Msa0527680	AT5G37780.1	44.595	74	41	0	74	147	73	146	1.15e-12	62.0
Msa0527680	AT3G43810.4	44.595	74	41	0	74	147	37	110	1.35e-12	60.8
Msa0527680	AT3G43810.3	44.595	74	41	0	74	147	37	110	1.35e-12	60.8
Msa0527680	AT1G66410.2	44.595	74	41	0	74	147	83	156	1.88e-12	61.6
Msa0527680	AT5G21274.1	44.595	74	41	0	74	147	73	146	2.12e-12	61.2
Msa0527680	AT1G73630.1	36.957	92	57	1	59	150	63	153	2.20e-12	61.6
Msa0527680	AT5G37780.4	44.595	74	41	0	74	147	60	133	2.34e-12	60.8
Msa0527680	AT3G56800.1	44.595	74	41	0	74	147	73	146	2.62e-12	61.2
Msa0527680	AT2G41110.1	44.595	74	41	0	74	147	73	146	2.62e-12	61.2
Msa0527680	AT2G27030.1	44.595	74	41	0	74	147	73	146	2.62e-12	61.2
Msa0527680	AT3G43810.1	44.595	74	41	0	74	147	73	146	2.73e-12	61.2
Msa0527680	AT5G37780.3	44.595	74	41	0	74	147	99	172	2.77e-12	61.6
Msa0527680	AT5G37780.2	44.595	74	41	0	74	147	88	161	3.06e-12	61.2
Msa0527680	AT2G41100.4	31.469	143	76	4	28	152	29	167	3.90e-12	62.8
Msa0527680	AT2G41100.1	31.469	143	76	4	28	152	29	167	3.90e-12	62.8
Msa0527680	AT2G27030.3	44.595	74	41	0	74	147	73	146	3.97e-12	61.2
Msa0527680	AT2G41110.2	44.595	74	41	0	74	147	85	158	4.19e-12	60.8
Msa0527680	AT2G43290.1	45.455	66	34	1	85	148	142	207	4.45e-12	61.6
Msa0527680	AT1G24620.1	48.438	64	33	0	84	147	108	171	1.15e-11	60.1
Msa0527680	AT4G20780.1	51.562	64	29	1	86	147	120	183	1.16e-11	60.1
Msa0527680	AT3G43810.2	44.595	74	41	0	74	147	73	146	1.25e-11	60.5
Msa0527680	AT2G41100.7	32.061	131	68	3	39	152	6	132	1.47e-11	61.2
Msa0527680	AT2G41100.6	32.061	131	68	3	39	152	6	132	1.47e-11	61.2
Msa0527680	AT2G41100.3	32.061	131	68	3	39	152	6	132	1.47e-11	61.2
Msa0527680	AT4G12860.1	47.541	61	30	1	90	148	82	142	1.90e-11	58.9
Msa0527680	AT1G66400.1	38.554	83	51	0	65	147	69	151	2.26e-11	58.9

Find 25 sgRNAs with CRISPR-Local

Find 27 sgRNAs with CRISPR-GE

CRISPR-Local

CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
ATTGCTCATATGTTTGAAAA+TGG	0.169368	4_1:-160805	Msa0527680:CDS
GTTTGAAAATGGGGTTAGTT+TGG	0.294711	4_1:-160794	Msa0527680:CDS
ATTAATGCAGTTGATCAAAA+TGG	0.313664	4_1:-160646	Msa0527680:CDS
CTATGAGTTTGTTGTGTTGA+TGG	0.336088	4_1:-160605	Msa0527680:CDS
AAGAGAGTCTTGTGTTGTTT+GGG	0.336625	4_1:-160703	Msa0527680:CDS
GAAGAGAGTCTTGTGTTGTT+TGG	0.378049	4_1:-160704	Msa0527680:CDS
TTGCTCATATGTTTGAAAAT+GGG	0.395795	4_1:-160804	Msa0527680:CDS
GGATTAAGAGTGGAGTTTGA+TGG	0.422050	4_1:-160862	Msa0527680:CDS
GTGTTGATGGAACAAAGTTT+TGG	0.423873	4_1:-160592	Msa0527680:CDS
GGGGATTGTATAGATGAGTT+TGG	0.426479	4_1:-160841	Msa0527680:CDS
TAATCTTGAACAAGTGAAGC+AGG	0.448279	4_1:-160956	Msa0527680:CDS
CATTGTTGTGATGGAAAAGC+TGG	0.452065	4_1:-160884	Msa0527680:CDS
GATTAAGAGTGGAGTTTGAT+GGG	0.458447	4_1:-160861	Msa0527680:CDS
TGCTCATATGTTTGAAAATG+GGG	0.468776	4_1:-160803	Msa0527680:CDS
GTGTTTGATGAAAACAAAGA+TGG	0.498276	4_1:-160748	Msa0527680:CDS
AGAAGAAATCATTGTTGTGA+TGG	0.498467	4_1:-160893	Msa0527680:CDS
AAACAAAGATGGGTTTATAG+AGG	0.531105	4_1:-160737	Msa0527680:CDS
TGTTTGATGAAAACAAAGAT+GGG	0.535462	4_1:-160747	Msa0527680:CDS
GCAGTTGATCAAAATGGAGA+TGG	0.549331	4_1:-160640	Msa0527680:CDS
GGAAAAGCTGGGATTAAGAG+TGG	0.561846	4_1:-160872	Msa0527680:CDS
AATCTTGAACAAGTGAAGCA+GGG	0.597395	4_1:-160955	Msa0527680:CDS
TCAACACAACAAACTCATAG+TGG	0.604767	4_1:+160608	None:intergenic
ATTAAGAGTGGAGTTTGATG+GGG	0.610187	4_1:-160860	Msa0527680:CDS
ATTGTTGTGATGGAAAAGCT+GGG	0.629690	4_1:-160883	Msa0527680:CDS
TTGAATAGCTGCATTCAAAG+TGG	0.646037	4_1:-160931	Msa0527680:CDS

CRISPR-GE

badsite warning	sgRNA_sequence	Strand	Position	Region	GC_content
!!!	ATGTTTTCTTTTTCTACAAT+TGG	-	chr4_1:160598-160617	Msa0527680:CDS	20.0%
!	ATTGCTCATATGTTTGAAAA+TGG	-	chr4_1:160807-160826	Msa0527680:CDS	25.0%
!	TTGCTCATATGTTTGAAAAT+GGG	-	chr4_1:160808-160827	Msa0527680:CDS	25.0%
!	TGTTTGATGAAAACAAAGAT+GGG	-	chr4_1:160865-160884	Msa0527680:CDS	25.0%
!	ATTAATGCAGTTGATCAAAA+TGG	-	chr4_1:160966-160985	Msa0527680:CDS	25.0%
	AGAAGAAATCATTGTTGTGA+TGG	-	chr4_1:160719-160738	Msa0527680:CDS	30.0%
	TGCTCATATGTTTGAAAATG+GGG	-	chr4_1:160809-160828	Msa0527680:CDS	30.0%
	GTGTTTGATGAAAACAAAGA+TGG	-	chr4_1:160864-160883	Msa0527680:CDS	30.0%
!	AAACAAAGATGGGTTTATAG+AGG	-	chr4_1:160875-160894	Msa0527680:CDS	30.0%
	TAATCTTGAACAAGTGAAGC+AGG	-	chr4_1:160656-160675	Msa0527680:CDS	35.0%
	AATCTTGAACAAGTGAAGCA+GGG	-	chr4_1:160657-160676	Msa0527680:CDS	35.0%
	TTGAATAGCTGCATTCAAAG+TGG	-	chr4_1:160681-160700	Msa0527680:CDS	35.0%
!	ATTGTTGTGATGGAAAAGCT+GGG	-	chr4_1:160729-160748	Msa0527680:CDS	35.0%
	GATTAAGAGTGGAGTTTGAT+GGG	-	chr4_1:160751-160770	Msa0527680:CDS	35.0%
	ATTAAGAGTGGAGTTTGATG+GGG	-	chr4_1:160752-160771	Msa0527680:CDS	35.0%
!!	GTTTGAAAATGGGGTTAGTT+TGG	-	chr4_1:160818-160837	Msa0527680:CDS	35.0%
!!	AAGAGAGTCTTGTGTTGTTT+GGG	-	chr4_1:160909-160928	Msa0527680:CDS	35.0%
	TCAACACAACAAACTCATAG+TGG	+	chr4_1:161007-161026	None:intergenic	35.0%
!!	CTATGAGTTTGTTGTGTTGA+TGG	-	chr4_1:161007-161026	Msa0527680:CDS	35.0%
!!	GTGTTGATGGAACAAAGTTT+TGG	-	chr4_1:161020-161039	Msa0527680:CDS	35.0%
!	CATTGTTGTGATGGAAAAGC+TGG	-	chr4_1:160728-160747	Msa0527680:CDS	40.0%
	GGATTAAGAGTGGAGTTTGA+TGG	-	chr4_1:160750-160769	Msa0527680:CDS	40.0%
	GGGGATTGTATAGATGAGTT+TGG	-	chr4_1:160771-160790	Msa0527680:CDS	40.0%
!	GAAGAGAGTCTTGTGTTGTT+TGG	-	chr4_1:160908-160927	Msa0527680:CDS	40.0%
!	GGGATTGCATAGAGATTTTG+TGG	-	chr4_1:160929-160948	Msa0527680:CDS	40.0%
	GCAGTTGATCAAAATGGAGA+TGG	-	chr4_1:160972-160991	Msa0527680:CDS	40.0%
	GGAAAAGCTGGGATTAAGAG+TGG	-	chr4_1:160740-160759	Msa0527680:CDS	45.0%

Chromosome	Type	Strat	End	Strand	Name
chr4_1	gene	160588	161046	160588	ID=Msa0527680;Name=Msa0527680
chr4_1	mRNA	160588	161046	160588	ID=Msa0527680-mRNA-1;Parent=Msa0527680;Name=Msa0527680-mRNA-1;_AED=0.04;_eAED=0.04;_QI=0\|-1\|0\|1\|-1\|1\|1\|0\|152
chr4_1	exon	160588	161046	160588	ID=Msa0527680-mRNA-1:exon:7;Parent=Msa0527680-mRNA-1
chr4_1	CDS	160588	161046	160588	ID=Msa0527680-mRNA-1:cds;Parent=Msa0527680-mRNA-1

Gene Sequence

>Msa0527680

TTGTGGAACTATGTTTTCTTTTTCTACAATTGGAAGTTATGCAATTGCATCACTCTTTCAAGCTACTCTAATCTTGAACAAGTGAAGCAGGGTTTGAATAGCTGCATTCAAAGTGGTGAGAAACTGTGCAAAGAAGAAATCATTGTTGTGATGGAAAAGCTGGGATTAAGAGTGGAGTTTGATGGGGATTGTATAGATGAGTTTGGTGATGAACAAGAAATTGCTCATATGTTTGAAAATGGGGTTAGTTTGGAAGAGTTGAATGAAGCTTTCAATGTGTTTGATGAAAACAAAGATGGGTTTATAGAGGCTGCTGAGTTGAAGAGAGTCTTGTGTTGTTTGGGATTGCATAGAGATTTTGTGGAGTGTCAGAAAATGATTAATGCAGTTGATCAAAATGGAGATGGATTAATTGACCACTATGAGTTTGTTGTGTTGATGGAACAAAGTTTTGGTTGA

Protein sequence

>Msa0527680

LWNYVFFFYNWKLCNCITLSSYSNLEQVKQGLNSCIQSGEKLCKEEIIVVMEKLGLRVEFDGDCIDEFGDEQEIAHMFENGVSLEELNEAFNVFDENKDGFIEAAELKRVLCCLGLHRDFVECQKMINAVDQNGDGLIDHYEFVVLMEQSFG*