AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa0570370

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0570370 MtrunA17_Chr7g0269241 82.000 150 1 1 1 124 1 150 6.68e-79 229
Msa0570370 MtrunA17_Chr8g0382851 80.667 150 3 1 1 124 1 150 3.42e-77 224
Msa0570370 MtrunA17_Chr1g0195711 67.470 83 19 1 1 75 1 83 6.28e-34 114
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0570370 AT5G27720.1 58.621 145 13 2 1 119 1 124 1.93e-32 111

Find 89 sgRNAs with CRISPR-Local

Find 219 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
CACCCCAACCTTGGTGTATT+TGG 0.097678 4_1:+72532788 Msa0570370:three_prime_UTR
GTTTCATCTACAATTGTTAA+AGG 0.239961 4_1:-72532817 None:intergenic
AATGTTTGATTTAAAATGTT+TGG 0.317969 4_1:+72532934 Msa0570370:three_prime_UTR
ACCCCAACCTTGGTGTATTT+GGG 0.324236 4_1:+72532789 Msa0570370:three_prime_UTR
AGGGAGGATGCTGCTGGTGC+TGG 0.332056 4_1:+72531805 Msa0570370:CDS
GATGGTGGGGCTAGAGGATC+TGG 0.334979 4_1:+72531865 Msa0570370:CDS
TAAATCAAACATTCAACTTC+AGG 0.337589 4_1:-72532924 None:intergenic
ACCTGGTGTGGGACGTGGTA+GGG 0.379220 4_1:+72531774 Msa0570370:CDS
ACTCTCTAAATCTTCCTTCC+TGG 0.392472 4_1:+72529805 Msa0570370:five_prime_UTR
CAATTGTAGATGAAACGTTC+GGG 0.397168 4_1:+72532824 Msa0570370:three_prime_UTR
GTAGGTGCTGGGCGGGGTAG+AGG 0.398941 4_1:+72532464 Msa0570370:intron
TTCCTCCAGGCTTTCCACCT+TGG 0.401016 4_1:-72531906 None:intergenic
CCTGGTGTGGGACGTGGTAG+GGG 0.408573 4_1:+72531775 Msa0570370:CDS
TTAGGTTATCGACAAAGTTC+AGG 0.410051 4_1:+72531247 Msa0570370:intron
ACAATTGTAGATGAAACGTT+CGG 0.413514 4_1:+72532823 Msa0570370:three_prime_UTR
TAACAAACGTCTAAATTACT+AGG 0.413684 4_1:-72532869 None:intergenic
GAGGATCTGGAGGCCGAGGC+AGG 0.419139 4_1:+72531878 Msa0570370:CDS
ATCCCAAATACACCAAGGTT+GGG 0.420252 4_1:-72532791 None:intergenic
GACCGTGTTTCATGTTTCCT+TGG 0.421478 4_1:+72532606 Msa0570370:three_prime_UTR
TGGGGTGCTCTTCTTACTGC+GGG 0.422623 4_1:-72532772 None:intergenic
AGAGACATATAATGGGCATT+TGG 0.429273 4_1:+72530515 Msa0570370:CDS
GATTTACCTCTGCTTCCTCC+AGG 0.434977 4_1:-72531919 None:intergenic
CCGAGGAAGGACTCTGTCAT+TGG 0.437402 4_1:+72532699 Msa0570370:three_prime_UTR
CACCTTGGCTAGCCCTGCCT+CGG 0.439949 4_1:-72531891 None:intergenic
ACCATGTTGATGTTGATGAT+TGG 0.450540 4_1:-72530157 None:intergenic
CAGGAAGAAAAGAGCCGTAC+AGG 0.450547 4_1:+72531266 Msa0570370:CDS
GCTAGCCAAGGTGGAAAGCC+TGG 0.450993 4_1:+72531901 Msa0570370:CDS
GACGTGGTAGGGGTAGAGGT+AGG 0.454494 4_1:+72531785 Msa0570370:CDS
CACCTGGTGTGGGACGTGGT+AGG 0.455282 4_1:+72531773 Msa0570370:CDS
AGAGGTAGGGAGGATGCTGC+TGG 0.457443 4_1:+72531799 Msa0570370:CDS
GTGGGACGTGGTAGGGGTAG+AGG 0.457910 4_1:+72531781 Msa0570370:CDS
TAGAGGTACAAATAACTTCT+CGG 0.459260 4_1:-72530569 None:intergenic
CTTCTTTGCTCTACTTTCAG+AGG 0.464203 4_1:+72532539 Msa0570370:three_prime_UTR
GCTAGAGGATCTGGAGGCCG+AGG 0.478171 4_1:+72531874 Msa0570370:CDS
GCTGGTGGACGCCAAACAAA+AGG 0.478487 4_1:+72531823 Msa0570370:CDS
CATCCCAAATACACCAAGGT+TGG 0.484792 4_1:-72532792 None:intergenic
AAGCCACCTGGTGTGGGACG+TGG 0.485336 4_1:+72531769 Msa0570370:CDS
ATTTGGTTAATTGCGATACA+TGG 0.494307 4_1:+72530532 Msa0570370:CDS
TTTGATGATGGTGGGGCTAG+AGG 0.497048 4_1:+72531859 Msa0570370:CDS
ACGTGGTAGGGGTAGAGGTA+GGG 0.508609 4_1:+72531786 Msa0570370:CDS
CCCCTACCACGTCCCACACC+AGG 0.510334 4_1:-72531775 None:intergenic
GGACGCCAAACAAAAGGCAC+TGG 0.511420 4_1:+72531829 Msa0570370:CDS
AAAATGGAGAGACATATAAT+GGG 0.512411 4_1:+72530508 Msa0570370:CDS
GAGTGAGAAGCAAGATCTGA+AGG 0.513817 4_1:-72529786 None:intergenic
GAGGATGCTGCTGGTGCTGG+TGG 0.518067 4_1:+72531808 Msa0570370:CDS
GCAGTCTTAAGAAGAGAAAG+AGG 0.523025 4_1:-72530344 None:intergenic
CAAACAAAAGGCACTGGGCG+TGG 0.536007 4_1:+72531835 Msa0570370:CDS
AGATCGCAAGCCACCTGGTG+TGG 0.544354 4_1:+72531762 Msa0570370:intron
GAAGTGTGAACTACTTCCAA+TGG 0.545305 4_1:+72532507 Msa0570370:three_prime_UTR
AATCAATCAATCACTAAGTG+AGG 0.552219 4_1:+72530050 Msa0570370:five_prime_UTR
TGGTAGGGGTAGAGGTAGGG+AGG 0.553128 4_1:+72531789 Msa0570370:CDS
GATGAGAGAGGTTACCAGGA+AGG 0.553877 4_1:-72529819 None:intergenic
TCCCAAATACACCAAGGTTG+GGG 0.555538 4_1:-72532790 None:intergenic
GACTGCACAAGGCCACCCTA+TGG 0.555565 4_1:+72530361 Msa0570370:CDS
GAGCAGAACGTGAGAGAAAG+CGG 0.556325 4_1:-72530088 None:intergenic
AAGTGTGAACTACTTCCAAT+GGG 0.558652 4_1:+72532508 Msa0570370:three_prime_UTR
GATCGCAAGCCACCTGGTGT+GGG 0.560007 4_1:+72531763 Msa0570370:intron
AATAATACTTCGTACCTGTA+CGG 0.560276 4_1:-72531280 None:intergenic
TAAGAAGAGCACCCCAACCT+TGG 0.563862 4_1:+72532779 Msa0570370:three_prime_UTR
TTCTTACTGCGGGGAAAGAG+AGG 0.565244 4_1:-72532762 None:intergenic
AAAGAAGCAAACTCGCCCAT+TGG 0.573700 4_1:-72532523 None:intergenic
GGCCGAGGCAGGGCTAGCCA+AGG 0.574778 4_1:+72531889 Msa0570370:CDS
TACTTTAAAGCTAAAATCCA+AGG 0.574793 4_1:-72532623 None:intergenic
CGGTCAGTGAAGTCTGCTGC+TGG 0.581651 4_1:-72532588 None:intergenic
GGAAAGCCTGGAGGAAGCAG+AGG 0.582079 4_1:+72531913 Msa0570370:CDS
AGGATCTGGAGGCCGAGGCA+GGG 0.591992 4_1:+72531879 Msa0570370:CDS
ATCAATCAATCACTAAGTGA+GGG 0.600982 4_1:+72530051 Msa0570370:five_prime_UTR
CCAATGACAGAGTCCTTCCT+CGG 0.605856 4_1:-72532699 None:intergenic
TCTTACTGCGGGGAAAGAGA+GGG 0.609601 4_1:-72532761 None:intergenic
TTGGGGTGCTCTTCTTACTG+CGG 0.615739 4_1:-72532773 None:intergenic
CAATTTCTAATACCTTCCAA+TGG 0.618428 4_1:+72532724 Msa0570370:three_prime_UTR
TCTCTTCTTAAGACTGCACA+AGG 0.621157 4_1:+72530350 Msa0570370:CDS
CGAGGCAGGGCTAGCCAAGG+TGG 0.621999 4_1:+72531892 Msa0570370:CDS
GACGCCAAACAAAAGGCACT+GGG 0.628038 4_1:+72531830 Msa0570370:CDS
TATTATATATTATCCGAGGA+AGG 0.630667 4_1:+72532686 Msa0570370:three_prime_UTR
GTGCTATTATATATTATCCG+AGG 0.634632 4_1:+72532682 Msa0570370:three_prime_UTR
CAGAACGTGAGAGAAAGCGG+AGG 0.636931 4_1:-72530085 None:intergenic
GGTGGGGCTAGAGGATCTGG+AGG 0.638366 4_1:+72531868 Msa0570370:CDS
TCAAACATTCAACTTCAGGA+AGG 0.652398 4_1:-72532920 None:intergenic
GCGTCAGTTTCATTCATACA+CGG 0.657543 4_1:-72532994 None:intergenic
CTACCACGTCCCACACCAGG+TGG 0.667375 4_1:-72531772 None:intergenic
ACCAATCATCAACATCAACA+TGG 0.675278 4_1:+72530156 Msa0570370:exon
ATCCAAGGAAACATGAAACA+CGG 0.676425 4_1:-72532608 None:intergenic
AATTGTAGATGAAACGTTCG+GGG 0.698304 4_1:+72532825 Msa0570370:three_prime_UTR
ATTGTAGATGAAACGTTCGG+GGG 0.703684 4_1:+72532826 Msa0570370:three_prime_UTR
GGGGTGCTCTTCTTACTGCG+GGG 0.710639 4_1:-72532771 None:intergenic
TTGTAGATGAAACGTTCGGG+GGG 0.711614 4_1:+72532827 Msa0570370:three_prime_UTR
AGCCAAGGTGGAAAGCCTGG+AGG 0.717081 4_1:+72531904 Msa0570370:CDS
GGAGCATCCCAAATACACCA+AGG 0.726143 4_1:-72532796 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! AAATAAATAAATAAATAAAA+AGG - chr4_1:72530400-72530419 None:intergenic 0.0%
!!! TATTTATTTATTTTACTTTG+TGG + chr4_1:72530407-72530426 Msa0570370:intron 10.0%
!!! TTATTTTATAAAGAAATCAA+GGG + chr4_1:72530804-72530823 Msa0570370:intron 10.0%
!!! TTTTCTTAAATAACATAAAA+GGG - chr4_1:72531001-72531020 None:intergenic 10.0%
!!! AACTTTATTTTTTATTTAAC+TGG - chr4_1:72531719-72531738 None:intergenic 10.0%
!! AAAATGCATAAAAATTATAT+AGG + chr4_1:72532371-72532390 Msa0570370:intron 10.0%
!!! TTTAATTTGTTATTGTTGAA+AGG + chr4_1:72530635-72530654 Msa0570370:intron 15.0%
!!! TTATGAATTTTGATTGAAAA+TGG + chr4_1:72530663-72530682 Msa0570370:intron 15.0%
!!! TATGAATTTTGATTGAAAAT+GGG + chr4_1:72530664-72530683 Msa0570370:intron 15.0%
!!! GTTATTTTATAAAGAAATCA+AGG + chr4_1:72530803-72530822 Msa0570370:intron 15.0%
!!! GTTTTCTTAAATAACATAAA+AGG - chr4_1:72531002-72531021 None:intergenic 15.0%
!! AATCAGAAGAAAATTATATA+TGG - chr4_1:72531596-72531615 None:intergenic 15.0%
!! TGAAAATAAAATATAATACC+AGG - chr4_1:72532229-72532248 None:intergenic 15.0%
!!! AATGTTTGATTTAAAATGTT+TGG + chr4_1:72532934-72532953 Msa0570370:three_prime_UTR 15.0%
!!! AATTAAAACCTCTGAAAAAT+TGG - chr4_1:72529757-72529776 None:intergenic 20.0%
!! TTTAAACTAATGATATGAAG+TGG - chr4_1:72530883-72530902 None:intergenic 20.0%
!!! ATGTTATTTAAGAAAACCAT+TGG + chr4_1:72531005-72531024 Msa0570370:intron 20.0%
!! AATTATCTGATTCACTAAAA+GGG - chr4_1:72531167-72531186 None:intergenic 20.0%
!! AAATTATCTGATTCACTAAA+AGG - chr4_1:72531168-72531187 None:intergenic 20.0%
!! TATTTCAATCCAAAATCTAA+AGG - chr4_1:72531430-72531449 None:intergenic 20.0%
!!! TTAGATTTTGGATTGAAATA+TGG + chr4_1:72531430-72531449 Msa0570370:intron 20.0%
!!! ATTTTTCTTGTATTTATCCA+CGG + chr4_1:72532645-72532664 Msa0570370:three_prime_UTR 20.0%
!!! TCTTAATTCCAATTTTTCAG+AGG + chr4_1:72529746-72529765 Msa0570370:five_prime_UTR 25.0%
! TCATGTTTCTCTATAAATAC+AGG + chr4_1:72530007-72530026 Msa0570370:intron 25.0%
! CATGTTTCTCTATAAATACA+GGG + chr4_1:72530008-72530027 Msa0570370:intron 25.0%
! ATGTTTCTCTATAAATACAG+GGG + chr4_1:72530009-72530028 Msa0570370:intron 25.0%
! TTTAACAAAATTGAACCACT+AGG + chr4_1:72530242-72530261 Msa0570370:intron 25.0%
! TTAACAAAATTGAACCACTA+GGG + chr4_1:72530243-72530262 Msa0570370:intron 25.0%
! AAAAATGGAGAGACATATAA+TGG + chr4_1:72530507-72530526 Msa0570370:CDS 25.0%
! AAAATGGAGAGACATATAAT+GGG + chr4_1:72530508-72530527 Msa0570370:CDS 25.0%
!!! AAAAAAGAGCATTACTTTAG+AGG - chr4_1:72530589-72530608 None:intergenic 25.0%
! TGATAAATCCTAAATTGACT+TGG + chr4_1:72531354-72531373 Msa0570370:intron 25.0%
! ACAACAATATTCATGTATCA+AGG - chr4_1:72531555-72531574 None:intergenic 25.0%
! AAACTCTTTGAACTATGATA+TGG - chr4_1:72532206-72532225 None:intergenic 25.0%
!! AACAATAATAACCTTTTACG+CGG - chr4_1:72532291-72532310 None:intergenic 25.0%
! TTACCAATTCTGTAATTTGA+CGG + chr4_1:72532322-72532341 Msa0570370:intron 25.0%
!! TCAAATACCTTTTCTTCTTT+TGG + chr4_1:72532442-72532461 Msa0570370:intron 25.0%
!!! TACTTTAAAGCTAAAATCCA+AGG - chr4_1:72532626-72532645 None:intergenic 25.0%
! TATTATATATTATCCGAGGA+AGG + chr4_1:72532686-72532705 Msa0570370:three_prime_UTR 25.0%
! GTTTCATCTACAATTGTTAA+AGG - chr4_1:72532820-72532839 None:intergenic 25.0%
! TAACAAACGTCTAAATTACT+AGG - chr4_1:72532872-72532891 None:intergenic 25.0%
! TAAATCAAACATTCAACTTC+AGG - chr4_1:72532927-72532946 None:intergenic 25.0%
!! AGCAACTTTTATCAAATTCA+TGG - chr4_1:72532965-72532984 None:intergenic 25.0%
GAAGAAAAAAGTGAGAATCA+GGG - chr4_1:72529863-72529882 None:intergenic 30.0%
AGAAGAAAAAAGTGAGAATC+AGG - chr4_1:72529864-72529883 None:intergenic 30.0%
!!! TTTTTTTTTGCTGCAACACT+TGG + chr4_1:72529957-72529976 Msa0570370:intron 30.0%
AATCAATCAATCACTAAGTG+AGG + chr4_1:72530050-72530069 Msa0570370:five_prime_UTR 30.0%
ATCAATCAATCACTAAGTGA+GGG + chr4_1:72530051-72530070 Msa0570370:five_prime_UTR 30.0%
GTAACTACTTTACCTTTCAA+AGG + chr4_1:72530178-72530197 Msa0570370:intron 30.0%
!! GGTTTTTGTTACTTACCATA+GGG - chr4_1:72530379-72530398 None:intergenic 30.0%
!! AGGTTTTTGTTACTTACCAT+AGG - chr4_1:72530380-72530399 None:intergenic 30.0%
!! TTTGAACTTGTTGTAACAGT+TGG + chr4_1:72530476-72530495 Msa0570370:intron 30.0%
ATTTGGTTAATTGCGATACA+TGG + chr4_1:72530532-72530551 Msa0570370:CDS 30.0%
! TAGAGGTACAAATAACTTCT+CGG - chr4_1:72530572-72530591 None:intergenic 30.0%
!! ATTTGTTATTGTTGAAAGGC+TGG + chr4_1:72530639-72530658 Msa0570370:intron 30.0%
TTGATTGAAAATGGGAATGT+AGG + chr4_1:72530672-72530691 Msa0570370:intron 30.0%
GCAATAGTAGATTGATATGT+TGG + chr4_1:72530919-72530938 Msa0570370:intron 30.0%
AGATTGATATGTTGGAATAG+TGG + chr4_1:72530927-72530946 Msa0570370:intron 30.0%
!! TTGGAATAGTGGATTCTTAT+TGG + chr4_1:72530938-72530957 Msa0570370:intron 30.0%
TAAGAAAACCATTGGTGAAT+AGG + chr4_1:72531013-72531032 Msa0570370:intron 30.0%
! TGCTTTATTGCCTTTGATTT+AGG + chr4_1:72531229-72531248 Msa0570370:intron 30.0%
TTGTCGATAACCTAAATCAA+AGG - chr4_1:72531242-72531261 None:intergenic 30.0%
AATAATACTTCGTACCTGTA+CGG - chr4_1:72531283-72531302 None:intergenic 30.0%
!! AATCCTAAATTGACTTGGAA+TGG + chr4_1:72531359-72531378 Msa0570370:intron 30.0%
!! ATCCTAAATTGACTTGGAAT+GGG + chr4_1:72531360-72531379 Msa0570370:intron 30.0%
AAGCTGAGTAAGATATAAAG+TGG + chr4_1:72531384-72531403 Msa0570370:intron 30.0%
! AATCTGACACCTTTAGATTT+TGG + chr4_1:72531418-72531437 Msa0570370:intron 30.0%
! ATGGTGAATTTGAGGTATTA+GGG - chr4_1:72532187-72532206 None:intergenic 30.0%
! TATGGTGAATTTGAGGTATT+AGG - chr4_1:72532188-72532207 None:intergenic 30.0%
ACTATGATATGGTGAATTTG+AGG - chr4_1:72532195-72532214 None:intergenic 30.0%
! ATACCTTTTCTTCTTTTGGT+AGG + chr4_1:72532446-72532465 Msa0570370:intron 30.0%
! GTGCTATTATATATTATCCG+AGG + chr4_1:72532682-72532701 Msa0570370:three_prime_UTR 30.0%
CAATTTCTAATACCTTCCAA+TGG + chr4_1:72532724-72532743 Msa0570370:three_prime_UTR 30.0%
! AAAAAACTTTTGCCATTGGA+AGG - chr4_1:72532739-72532758 None:intergenic 30.0%
! GGAAAAAAAACTTTTGCCAT+TGG - chr4_1:72532743-72532762 None:intergenic 30.0%
ACAATTGTAGATGAAACGTT+CGG + chr4_1:72532823-72532842 Msa0570370:three_prime_UTR 30.0%
ACCAATCATCAACATCAACA+TGG + chr4_1:72530156-72530175 Msa0570370:exon 35.0%
!! ACCATGTTGATGTTGATGAT+TGG - chr4_1:72530160-72530179 None:intergenic 35.0%
!! ATTCACTGTTAGCCTTTGAA+AGG - chr4_1:72530193-72530212 None:intergenic 35.0%
GACTATAACATAAACCCTAG+TGG - chr4_1:72530260-72530279 None:intergenic 35.0%
!! TTTTGTTACTTACCATAGGG+TGG - chr4_1:72530376-72530395 None:intergenic 35.0%
!! AGAGACATATAATGGGCATT+TGG + chr4_1:72530515-72530534 Msa0570370:CDS 35.0%
TTGAAAATGGGAATGTAGGA+TGG + chr4_1:72530676-72530695 Msa0570370:intron 35.0%
! TGTAGGATGGAGATAGATTT+TGG + chr4_1:72530689-72530708 Msa0570370:intron 35.0%
AACCTTACCTTGTGATCTAT+TGG + chr4_1:72531050-72531069 Msa0570370:intron 35.0%
TATTGGTCATGGGTTTAGTT+GGG + chr4_1:72531067-72531086 Msa0570370:intron 35.0%
TTAGGTTATCGACAAAGTTC+AGG + chr4_1:72531247-72531266 Msa0570370:intron 35.0%
! ACACTACAGAGATGTAGTTT+TGG - chr4_1:72531467-72531486 None:intergenic 35.0%
! ACTACATCTCTGTAGTGTTT+TGG + chr4_1:72531469-72531488 Msa0570370:intron 35.0%
!! TGTTGGGTTTGTTACTTATG+AGG - chr4_1:72531498-72531517 None:intergenic 35.0%
! TCTTCTGATTGATTCTGACT+TGG + chr4_1:72531606-72531625 Msa0570370:intron 35.0%
! CTTCTGATTGATTCTGACTT+GGG + chr4_1:72531607-72531626 Msa0570370:intron 35.0%
! TTCTGATTGATTCTGACTTG+GGG + chr4_1:72531608-72531627 Msa0570370:intron 35.0%
AATGGCATTTCAACTTGTGT+GGG - chr4_1:72531684-72531703 None:intergenic 35.0%
AAATGGCATTTCAACTTGTG+TGG - chr4_1:72531685-72531704 None:intergenic 35.0%
AACTGGAAGACTGCATAAAA+TGG - chr4_1:72531702-72531721 None:intergenic 35.0%
AGATAGAAGAAAACACAGAG+AGG - chr4_1:72531973-72531992 None:intergenic 35.0%
TCATAGTTCAAAGAGTTTCC+TGG + chr4_1:72532208-72532227 Msa0570370:intron 35.0%
AGAAAGTTATTCCGCGTAAA+AGG + chr4_1:72532277-72532296 Msa0570370:intron 35.0%
GAACCGTCAAATTACAGAAT+TGG - chr4_1:72532328-72532347 None:intergenic 35.0%
AAGTGTGAACTACTTCCAAT+GGG + chr4_1:72532508-72532527 Msa0570370:three_prime_UTR 35.0%
ATCCAAGGAAACATGAAACA+CGG - chr4_1:72532611-72532630 None:intergenic 35.0%
ATCCACGGTCTCAAAAAATT+TGG + chr4_1:72532660-72532679 Msa0570370:three_prime_UTR 35.0%
CAATTGTAGATGAAACGTTC+GGG + chr4_1:72532824-72532843 Msa0570370:three_prime_UTR 35.0%
AATTGTAGATGAAACGTTCG+GGG + chr4_1:72532825-72532844 Msa0570370:three_prime_UTR 35.0%
TCAAACATTCAACTTCAGGA+AGG - chr4_1:72532923-72532942 None:intergenic 35.0%
ACTCTCTAAATCTTCCTTCC+TGG + chr4_1:72529805-72529824 Msa0570370:five_prime_UTR 40.0%
GAAGAGAAGAAAGATGAGAG+AGG - chr4_1:72529834-72529853 None:intergenic 40.0%
!! TTTTCTGATTCGTCGCAACA+TGG - chr4_1:72530217-72530236 None:intergenic 40.0%
GAGGAAGCTGCAACAAAAAA+AGG - chr4_1:72530328-72530347 None:intergenic 40.0%
GCAGTCTTAAGAAGAGAAAG+AGG - chr4_1:72530347-72530366 None:intergenic 40.0%
TCTCTTCTTAAGACTGCACA+AGG + chr4_1:72530350-72530369 Msa0570370:CDS 40.0%
!! GAACTTGTTGTAACAGTTGG+TGG + chr4_1:72530479-72530498 Msa0570370:intron 40.0%
! AGGATGGAGATAGATTTTGG+CGG + chr4_1:72530692-72530711 Msa0570370:intron 40.0%
TTTCCTCGTATGTAGCATTC+AGG - chr4_1:72530721-72530740 None:intergenic 40.0%
GTTCCTGATGAGGTATGTAA+TGG + chr4_1:72530760-72530779 Msa0570370:intron 40.0%
! AGGTATGTAATGGTGGTGAT+TGG + chr4_1:72530770-72530789 Msa0570370:intron 40.0%
CTTAGCAACCTATTCACCAA+TGG - chr4_1:72531024-72531043 None:intergenic 40.0%
TGGTGAATAGGTTGCTAAGA+AGG + chr4_1:72531025-72531044 Msa0570370:intron 40.0%
GACCAATAGATCACAAGGTA+AGG - chr4_1:72531055-72531074 None:intergenic 40.0%
ACCTTGTGATCTATTGGTCA+TGG + chr4_1:72531056-72531075 Msa0570370:intron 40.0%
CCTTGTGATCTATTGGTCAT+GGG + chr4_1:72531057-72531076 Msa0570370:intron 40.0%
CTATTGGTCATGGGTTTAGT+TGG + chr4_1:72531066-72531085 Msa0570370:intron 40.0%
TGCCCATTCCAAGTCAATTT+AGG - chr4_1:72531365-72531384 None:intergenic 40.0%
!! TCTAAAGGTGTCAGATTCAC+AGG - chr4_1:72531415-72531434 None:intergenic 40.0%
!!! CTACAGAGATGTAGTTTTGG+TGG - chr4_1:72531464-72531483 None:intergenic 40.0%
! TCCAACAGAGGAAGTTTGTT+GGG - chr4_1:72531514-72531533 None:intergenic 40.0%
ATCCAACAGAGGAAGTTTGT+TGG - chr4_1:72531515-72531534 None:intergenic 40.0%
TAGAAGAAAACACAGAGAGG+TGG - chr4_1:72531970-72531989 None:intergenic 40.0%
GCACCTACCAAAAGAAGAAA+AGG - chr4_1:72532452-72532471 None:intergenic 40.0%
! TTTCTTCTTTTGGTAGGTGC+TGG + chr4_1:72532452-72532471 Msa0570370:intron 40.0%
! TTCTTCTTTTGGTAGGTGCT+GGG + chr4_1:72532453-72532472 Msa0570370:intron 40.0%
GAAGTGTGAACTACTTCCAA+TGG + chr4_1:72532507-72532526 Msa0570370:three_prime_UTR 40.0%
CTTCTTTGCTCTACTTTCAG+AGG + chr4_1:72532539-72532558 Msa0570370:three_prime_UTR 40.0%
!!! CACCAAATTTTTTGAGACCG+TGG - chr4_1:72532665-72532684 None:intergenic 40.0%
ATCCCAAATACACCAAGGTT+GGG - chr4_1:72532794-72532813 None:intergenic 40.0%
ATTGTAGATGAAACGTTCGG+GGG + chr4_1:72532826-72532845 Msa0570370:three_prime_UTR 40.0%
GAGTGAGAAGCAAGATCTGA+AGG - chr4_1:72529789-72529808 None:intergenic 45.0%
GAAAGATGAGAGAGGTTACC+AGG - chr4_1:72529826-72529845 None:intergenic 45.0%
! TGCTGCAACACTTGGACTTT+TGG + chr4_1:72529965-72529984 Msa0570370:intron 45.0%
!! CAGTTGGTGGAGCTGAAAAA+TGG + chr4_1:72530492-72530511 Msa0570370:intron 45.0%
ATGCCTGAATGCTACATACG+AGG + chr4_1:72530715-72530734 Msa0570370:intron 45.0%
ATACCTCATCAGGAACTCGA+AGG - chr4_1:72530756-72530775 None:intergenic 45.0%
CCACCATTACATACCTCATC+AGG - chr4_1:72530766-72530785 None:intergenic 45.0%
CCTGATGAGGTATGTAATGG+TGG + chr4_1:72530763-72530782 Msa0570370:intron 45.0%
CCCATGACCAATAGATCACA+AGG - chr4_1:72531060-72531079 None:intergenic 45.0%
TGGGAAAAACCTTGTGCTTG+AGG + chr4_1:72531086-72531105 Msa0570370:intron 45.0%
GGGAAAAACCTTGTGCTTGA+GGG + chr4_1:72531087-72531106 Msa0570370:intron 45.0%
AGAGTATGAGCACAACCTTG+AGG - chr4_1:72531119-72531138 None:intergenic 45.0%
ACCCAACAAACTTCCTCTGT+TGG + chr4_1:72531510-72531529 Msa0570370:intron 45.0%
CTAAGTGCTAGATCCAACAG+AGG - chr4_1:72531526-72531545 None:intergenic 45.0%
AAAGAAGCAAACTCGCCCAT+TGG - chr4_1:72532526-72532545 None:intergenic 45.0%
GACCGTGTTTCATGTTTCCT+TGG + chr4_1:72532606-72532625 Msa0570370:three_prime_UTR 45.0%
! ACCCCAACCTTGGTGTATTT+GGG + chr4_1:72532789-72532808 Msa0570370:three_prime_UTR 45.0%
TCCCAAATACACCAAGGTTG+GGG - chr4_1:72532793-72532812 None:intergenic 45.0%
CATCCCAAATACACCAAGGT+TGG - chr4_1:72532795-72532814 None:intergenic 45.0%
TTGTAGATGAAACGTTCGGG+GGG + chr4_1:72532827-72532846 Msa0570370:three_prime_UTR 45.0%
GATGAGAGAGGTTACCAGGA+AGG - chr4_1:72529822-72529841 None:intergenic 50.0%
GAGCAGAACGTGAGAGAAAG+CGG - chr4_1:72530091-72530110 None:intergenic 50.0%
! GTACCTTCGAGTTCCTGATG+AGG + chr4_1:72530750-72530769 Msa0570370:intron 50.0%
GGCTTAAACCCTCAAGCACA+AGG - chr4_1:72531098-72531117 None:intergenic 50.0%
GCTTGAGGGTTTAAGCCTCA+AGG + chr4_1:72531101-72531120 Msa0570370:intron 50.0%
CAGGAAGAAAAGAGCCGTAC+AGG + chr4_1:72531266-72531285 Msa0570370:CDS 50.0%
! TTTTCAGATCGCAAGCCACC+TGG + chr4_1:72531757-72531776 Msa0570370:intron 50.0%
!! GACGCCAAACAAAAGGCACT+GGG + chr4_1:72531830-72531849 Msa0570370:CDS 50.0%
!!! ACTGGGCGTGGTTTTGATGA+TGG + chr4_1:72531847-72531866 Msa0570370:CDS 50.0%
!!! GGCGTGGTTTTGATGATGGT+GGG + chr4_1:72531851-72531870 Msa0570370:CDS 50.0%
!!! GCGTGGTTTTGATGATGGTG+GGG + chr4_1:72531852-72531871 Msa0570370:CDS 50.0%
!! TTTGATGATGGTGGGGCTAG+AGG + chr4_1:72531859-72531878 Msa0570370:CDS 50.0%
GATTTACCTCTGCTTCCTCC+AGG - chr4_1:72531922-72531941 None:intergenic 50.0%
!!! TTCTTTTGGTAGGTGCTGGG+CGG + chr4_1:72532456-72532475 Msa0570370:intron 50.0%
CCAATGACAGAGTCCTTCCT+CGG - chr4_1:72532702-72532721 None:intergenic 50.0%
TCTTACTGCGGGGAAAGAGA+GGG - chr4_1:72532764-72532783 None:intergenic 50.0%
TTCTTACTGCGGGGAAAGAG+AGG - chr4_1:72532765-72532784 None:intergenic 50.0%
! TTGGGGTGCTCTTCTTACTG+CGG - chr4_1:72532776-72532795 None:intergenic 50.0%
!! TAAGAAGAGCACCCCAACCT+TGG + chr4_1:72532779-72532798 Msa0570370:three_prime_UTR 50.0%
! CACCCCAACCTTGGTGTATT+TGG + chr4_1:72532788-72532807 Msa0570370:three_prime_UTR 50.0%
! GGAGCATCCCAAATACACCA+AGG - chr4_1:72532799-72532818 None:intergenic 50.0%
CAGAACGTGAGAGAAAGCGG+AGG - chr4_1:72530088-72530107 None:intergenic 55.0%
ACGTGGTAGGGGTAGAGGTA+GGG + chr4_1:72531786-72531805 Msa0570370:CDS 55.0%
! GCTGGTGGACGCCAAACAAA+AGG + chr4_1:72531823-72531842 Msa0570370:CDS 55.0%
! GGACGCCAAACAAAAGGCAC+TGG + chr4_1:72531829-72531848 Msa0570370:CDS 55.0%
! CACGCCCAGTGCCTTTTGTT+TGG - chr4_1:72531837-72531856 None:intergenic 55.0%
!! CAAACAAAAGGCACTGGGCG+TGG + chr4_1:72531835-72531854 Msa0570370:CDS 55.0%
!!! GGGCGTGGTTTTGATGATGG+TGG + chr4_1:72531850-72531869 Msa0570370:CDS 55.0%
TTCCTCCAGGCTTTCCACCT+TGG - chr4_1:72531909-72531928 None:intergenic 55.0%
!!! TCTTTTGGTAGGTGCTGGGC+GGG + chr4_1:72532457-72532476 Msa0570370:intron 55.0%
CCGAGGAAGGACTCTGTCAT+TGG + chr4_1:72532699-72532718 Msa0570370:three_prime_UTR 55.0%
! TGGGGTGCTCTTCTTACTGC+GGG - chr4_1:72532775-72532794 None:intergenic 55.0%
GACTGCACAAGGCCACCCTA+TGG + chr4_1:72530361-72530380 Msa0570370:CDS 60.0%
AGATCGCAAGCCACCTGGTG+TGG + chr4_1:72531762-72531781 Msa0570370:intron 60.0%
GATCGCAAGCCACCTGGTGT+GGG + chr4_1:72531763-72531782 Msa0570370:intron 60.0%
ACCTGGTGTGGGACGTGGTA+GGG + chr4_1:72531774-72531793 Msa0570370:CDS 60.0%
GACGTGGTAGGGGTAGAGGT+AGG + chr4_1:72531785-72531804 Msa0570370:CDS 60.0%
TGGTAGGGGTAGAGGTAGGG+AGG + chr4_1:72531789-72531808 Msa0570370:CDS 60.0%
AGAGGTAGGGAGGATGCTGC+TGG + chr4_1:72531799-72531818 Msa0570370:CDS 60.0%
!! GATGGTGGGGCTAGAGGATC+TGG + chr4_1:72531865-72531884 Msa0570370:CDS 60.0%
GCTAGCCAAGGTGGAAAGCC+TGG + chr4_1:72531901-72531920 Msa0570370:CDS 60.0%
AGCCAAGGTGGAAAGCCTGG+AGG + chr4_1:72531904-72531923 Msa0570370:CDS 60.0%
GGAAAGCCTGGAGGAAGCAG+AGG + chr4_1:72531913-72531932 Msa0570370:CDS 60.0%
!!! CTTTTGGTAGGTGCTGGGCG+GGG + chr4_1:72532458-72532477 Msa0570370:intron 60.0%
CGGTCAGTGAAGTCTGCTGC+TGG - chr4_1:72532591-72532610 None:intergenic 60.0%
! GGGGTGCTCTTCTTACTGCG+GGG - chr4_1:72532774-72532793 None:intergenic 60.0%
AAGCCACCTGGTGTGGGACG+TGG + chr4_1:72531769-72531788 Msa0570370:CDS 65.0%
CTACCACGTCCCACACCAGG+TGG - chr4_1:72531775-72531794 None:intergenic 65.0%
CACCTGGTGTGGGACGTGGT+AGG + chr4_1:72531773-72531792 Msa0570370:CDS 65.0%
CCTGGTGTGGGACGTGGTAG+GGG + chr4_1:72531775-72531794 Msa0570370:CDS 65.0%
GTGGGACGTGGTAGGGGTAG+AGG + chr4_1:72531781-72531800 Msa0570370:CDS 65.0%
AGGGAGGATGCTGCTGGTGC+TGG + chr4_1:72531805-72531824 Msa0570370:CDS 65.0%
! GAGGATGCTGCTGGTGCTGG+TGG + chr4_1:72531808-72531827 Msa0570370:CDS 65.0%
GGTGGGGCTAGAGGATCTGG+AGG + chr4_1:72531868-72531887 Msa0570370:CDS 65.0%
GCTAGAGGATCTGGAGGCCG+AGG + chr4_1:72531874-72531893 Msa0570370:CDS 65.0%
AGGATCTGGAGGCCGAGGCA+GGG + chr4_1:72531879-72531898 Msa0570370:CDS 65.0%
! CACCTTGGCTAGCCCTGCCT+CGG - chr4_1:72531894-72531913 None:intergenic 65.0%
CCCCTACCACGTCCCACACC+AGG - chr4_1:72531778-72531797 None:intergenic 70.0%
GAGGATCTGGAGGCCGAGGC+AGG + chr4_1:72531878-72531897 Msa0570370:CDS 70.0%
!! CGAGGCAGGGCTAGCCAAGG+TGG + chr4_1:72531892-72531911 Msa0570370:CDS 70.0%
!! GTAGGTGCTGGGCGGGGTAG+AGG + chr4_1:72532464-72532483 Msa0570370:intron 70.0%
!! GGCCGAGGCAGGGCTAGCCA+AGG + chr4_1:72531889-72531908 Msa0570370:CDS 75.0%
Chromosome Type Strat End Strand Name
chr4_1 gene 72529744 72533014 72529744 ID=Msa0570370;Name=Msa0570370
chr4_1 mRNA 72529744 72533014 72529744 ID=Msa0570370-mRNA-1;Parent=Msa0570370;Name=Msa0570370-mRNA-1;_AED=0.04;_eAED=0.04;_QI=229|0.83|1|1|0.66|0.57|7|524|124
chr4_1 exon 72529744 72529826 72529744 ID=Msa0570370-mRNA-1:exon:24783;Parent=Msa0570370-mRNA-1
chr4_1 exon 72530029 72530177 72530029 ID=Msa0570370-mRNA-1:exon:24784;Parent=Msa0570370-mRNA-1
chr4_1 exon 72530341 72530382 72530341 ID=Msa0570370-mRNA-1:exon:24785;Parent=Msa0570370-mRNA-1
chr4_1 exon 72530495 72530593 72530495 ID=Msa0570370-mRNA-1:exon:24786;Parent=Msa0570370-mRNA-1
chr4_1 exon 72531251 72531287 72531251 ID=Msa0570370-mRNA-1:exon:24787;Parent=Msa0570370-mRNA-1
chr4_1 exon 72531764 72531934 72531764 ID=Msa0570370-mRNA-1:exon:24788;Parent=Msa0570370-mRNA-1
chr4_1 exon 72532468 72533014 72532468 ID=Msa0570370-mRNA-1:exon:24789;Parent=Msa0570370-mRNA-1
chr4_1 five_prime_UTR 72529744 72529826 72529744 ID=Msa0570370-mRNA-1:five_prime_utr;Parent=Msa0570370-mRNA-1
chr4_1 five_prime_UTR 72530029 72530174 72530029 ID=Msa0570370-mRNA-1:five_prime_utr;Parent=Msa0570370-mRNA-1
chr4_1 CDS 72530175 72530177 72530175 ID=Msa0570370-mRNA-1:cds;Parent=Msa0570370-mRNA-1
chr4_1 CDS 72530341 72530382 72530341 ID=Msa0570370-mRNA-1:cds;Parent=Msa0570370-mRNA-1
chr4_1 CDS 72530495 72530593 72530495 ID=Msa0570370-mRNA-1:cds;Parent=Msa0570370-mRNA-1
chr4_1 CDS 72531251 72531287 72531251 ID=Msa0570370-mRNA-1:cds;Parent=Msa0570370-mRNA-1
chr4_1 CDS 72531764 72531934 72531764 ID=Msa0570370-mRNA-1:cds;Parent=Msa0570370-mRNA-1
chr4_1 CDS 72532468 72532490 72532468 ID=Msa0570370-mRNA-1:cds;Parent=Msa0570370-mRNA-1
chr4_1 three_prime_UTR 72532491 72533014 72532491 ID=Msa0570370-mRNA-1:three_prime_utr;Parent=Msa0570370-mRNA-1
Gene Sequence

>Msa0570370

ATGCTTCCTCTTTCTCTTCTTAAGACTGCACAAGGCCACCCTATGTTGGTGGAGCTGAAAAATGGAGAGACATATAATGGGCATTTGGTTAATTGCGATACATGGATGAACATTCATCTCCGAGAAGTTATTTGTACCTCTAAAGTTATCGACAAAGTTCAGGAAGAAAAGAGCCGTACAGATCGCAAGCCACCTGGTGTGGGACGTGGTAGGGGTAGAGGTAGGGAGGATGCTGCTGGTGCTGGTGGACGCCAAACAAAAGGCACTGGGCGTGGTTTTGATGATGGTGGGGCTAGAGGATCTGGAGGCCGAGGCAGGGCTAGCCAAGGTGGAAAGCCTGGAGGAAGCAGAGGTGCTGGGCGGGGTAGAGGATGA
Protein sequence

>Msa0570370

MLPLSLLKTAQGHPMLVELKNGETYNGHLVNCDTWMNIHLREVICTSKVIDKVQEEKSRTDRKPPGVGRGRGRGREDAAGAGGRQTKGTGRGFDDGGARGSGGRGRASQGGKPGGSRGAGRGRG*