AlfalfaGEDB | Msa0609350

M. sativa cultivar zhongmu-4 / Msa0609350

KEGG: K02493|K02979|K07767|K08767
GO: GO:0000028 GO:0002181 GO:0003674 GO:0003676 GO:0003723 GO:0003729 GO:0003735 GO:0005198 GO:0005488 GO:0005575 GO:0005618 GO:0005622 GO:0005623 GO:0005737 GO:0005783 GO:0005789 GO:0005791 GO:0005829 GO:0005840 GO:0005844 GO:0005886 GO:0006139 GO:0006364 GO:0006396 GO:0006412 GO:0006518 GO:0006725 GO:0006807 GO:0006996 GO:0007275 GO:0007444 GO:0008150 GO:0008152 GO:0009058 GO:0009059 GO:0009888 GO:0009987 GO:0010467 GO:0012505 GO:0015935 GO:0016020 GO:0016043 GO:0016070 GO:0016072 GO:0019538 GO:0022607 GO:0022613 GO:0022618 GO:0022626 GO:0022627 GO:0030312 GO:0030490 GO:0030867 GO:0031090 GO:0031984 GO:0032501 GO:0032502 GO:0032991 GO:0034470 GO:0034622 GO:0034641 GO:0034645 GO:0034660 GO:0035220 GO:0035295 GO:0042175 GO:0042254 GO:0042255 GO:0042274 GO:0042788 GO:0043043 GO:0043170 GO:0043226 GO:0043227 GO:0043228 GO:0043229 GO:0043231 GO:0043232 GO:0043603 GO:0043604 GO:0043933 GO:0044085 GO:0044237 GO:0044238 GO:0044249 GO:0044260 GO:0044267 GO:0044271 GO:0044391 GO:0044422 GO:0044424 GO:0044425 GO:0044432 GO:0044444 GO:0044445 GO:0044446 GO:0044464 GO:0046483 GO:0048513 GO:0048731 GO:0048856 GO:0060429 GO:0065003 GO:0070925 GO:0071704 GO:0071826 GO:0071840 GO:0071944 GO:0090304 GO:0097159 GO:0098552 GO:0098554 GO:0098556 GO:0098562 GO:0098588 GO:0098827 GO:1901360 GO:1901363 GO:1901564 GO:1901566 GO:1901576 GO:1990904
PFAM: PF01200.21

Nr:

Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa0609350	XP_024637297.1	93.846	65	4	0	1	65	1	65	2.34e-35	124

Swissprot:

Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa0609350	sp\|P46302\|RS28_MAIZE	89.231	65	7	0	1	65	1	65	6.92e-34	112

Trembl:

Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score

TFs/TRs:

Gene ID	Type	Classification

Protein Kinases:

Gene ID	Type	Classification

Network:

Co-expression Network

Gene1	Gene2	correlation coefficient	p_value	FDR
Msa0609350	Msa0649890	0.848788	4.901541e-60	-8.615850e-47
Msa0609350	Msa0663400	0.802496	5.458464e-49	-8.615850e-47
Msa0609350	Msa1385480	0.800503	1.397738e-48	-8.615850e-47
Msa0609350	Msa1463570	0.811370	7.243496e-51	-8.615850e-47
Msa0128330	Msa0609350	0.811396	7.148350e-51	-8.615850e-47
Msa0174580	Msa0609350	0.822180	2.719353e-53	-8.615850e-47
Msa0277600	Msa0609350	0.810772	9.763281e-51	-8.615850e-47
Msa0579830	Msa0609350	0.840064	1.088783e-57	-8.615850e-47

PPI

Gene1	Gene2	Type

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa0609350	MtrunA17_Chr4g0041861	93.846	65	4	0	1	65	1	65	2.15e-39	124
Msa0609350	MtrunA17_Chr6g0453811	90.769	65	6	0	1	65	1	65	1.27e-37	119
Msa0609350	MtrunA17_Chr6g0453911	75.385	65	15	1	1	65	1	64	1.26e-28	96.7
Msa0609350	MtrunA17_Chr8g0381481	78.846	52	11	0	1	52	1	52	1.36e-24	86.3

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa0609350	AT5G03850.1	87.692	65	7	1	1	65	1	64	2.67e-34	111
Msa0609350	AT3G10090.1	87.692	65	7	1	1	65	1	64	2.67e-34	111
Msa0609350	AT5G64140.1	87.692	65	7	1	1	65	1	64	5.23e-34	110

Find 36 sgRNAs with CRISPR-Local

Find 136 sgRNAs with CRISPR-GE

CRISPR-Local

CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
AGGGTGTCTCCTTCTCTAAC+TGG	0.224700	4_2:+56698023	None:intergenic
CCTCACTTGGTTGCGGTGGC+CGG	0.305223	4_2:+56698738	None:intergenic
TGCTGTTGTTGTCAAGGTTA+TGG	0.305748	4_2:-56698135	Msa0609350:CDS
CTCACTTGGTTGCGGTGGCC+GGG	0.328965	4_2:+56698739	None:intergenic
GCTGTTGTTGTCAAGGTTAT+GGG	0.344081	4_2:-56698134	Msa0609350:CDS
GAGGACAGGTGACTCAAGTT+AGG	0.354183	4_2:-56698097	Msa0609350:CDS
TTCCTCATGATGTGACGGTT+CGG	0.361521	4_2:+56698056	None:intergenic
CAAAATTCAACCAAATAAAA+AGG	0.363873	4_2:+56697844	None:intergenic
TCCTCATGATGTGACGGTTC+GGG	0.372907	4_2:+56698057	None:intergenic
CTTCTCTTTCAGACTCAAGC+AGG	0.393026	4_2:+56697997	None:intergenic
TCACTTGGTTGCGGTGGCCG+GGG	0.420954	4_2:+56698740	None:intergenic
GAAAGCATAAAGCGAAAAGT+AGG	0.435439	4_2:-56698814	Msa0609350:five_prime_UTR
GCTAGAGAAACTCAGCGGTT+TGG	0.474314	4_2:-56697962	Msa0609350:exon
GAAACTCAGCGGTTTGGTGC+AGG	0.476171	4_2:-56697956	Msa0609350:three_prime_UTR
GTCAAGGTTATGGGTCGTAC+TGG	0.486004	4_2:-56698125	Msa0609350:CDS
ATTGAGTGTTCTTCTTCCCC+CGG	0.494777	4_2:-56698757	Msa0609350:five_prime_UTR
TTTCAGACTCAAGCAGGGTG+AGG	0.496062	4_2:+56698003	None:intergenic
CACTTGGTTGCGGTGGCCGG+GGG	0.496936	4_2:+56698741	None:intergenic
GGGTGTCTCCTTCTCTAACT+GGG	0.522501	4_2:+56698024	None:intergenic
AGGACAGGTGACTCAAGTTA+GGG	0.535175	4_2:-56698096	Msa0609350:CDS
CAAGTATGCTGTTGTTGTCA+AGG	0.536125	4_2:-56698141	Msa0609350:CDS
TCGTACTGGTTCTAGAGGAC+AGG	0.538891	4_2:-56698111	Msa0609350:CDS
TTCAGACTCAAGCAGGGTGA+GGG	0.547860	4_2:+56698004	None:intergenic
ACCCGAACCGTCACATCATG+AGG	0.555528	4_2:-56698058	Msa0609350:CDS
AAAGCATAAAGCGAAAAGTA+GGG	0.572772	4_2:-56698813	Msa0609350:five_prime_UTR
GAAGAAACCTCACTTGGTTG+CGG	0.577435	4_2:+56698731	None:intergenic
GAAACCTCACTTGGTTGCGG+TGG	0.582952	4_2:+56698734	None:intergenic
TTCTCTTTCAGACTCAAGCA+GGG	0.585301	4_2:+56697998	None:intergenic
TCACATCATGAGGAATGTGA+AGG	0.587194	4_2:-56698048	Msa0609350:CDS
TCACATTCCTCATGATGTGA+CGG	0.589714	4_2:+56698051	None:intergenic
CACATCATGAGGAATGTGAA+GGG	0.593653	4_2:-56698047	Msa0609350:CDS
GTGAAGGGCCCAGTTAGAGA+AGG	0.598823	4_2:-56698032	Msa0609350:CDS
AGTCTGAAAGAGAAGCAAGA+AGG	0.599710	4_2:-56697989	Msa0609350:CDS
ATGGGTCGTACTGGTTCTAG+AGG	0.630812	4_2:-56698116	Msa0609350:CDS
CTCAATCAAGCTCTGCTCTG+CGG	0.700611	4_2:+56698774	None:intergenic
GTTGCGCTAGAGAAACTCAG+CGG	0.778395	4_2:-56697967	Msa0609350:exon

CRISPR-GE

badsite warning	sgRNA_sequence	Strand	Position	Region	GC_content
!!	TTAAAAAAAACAAAGAAAAA+AGG	+	chr4_2:56697973-56697992	None:intergenic	10.0%
!!	TTAAAAAAAACAAAGAAAAA+AGG	+	chr4_2:56697973-56697992	None:intergenic	10.0%
!!!	ATAAAAAACGATTTTCAATT+AGG	+	chr4_2:56698198-56698217	None:intergenic	15.0%
!!!	TTCAATAAACATTTGTTTTT+TGG	-	chr4_2:56698670-56698689	Msa0609350:intron	15.0%
!!!	TCAATAAACATTTGTTTTTT+GGG	-	chr4_2:56698671-56698690	Msa0609350:intron	15.0%
!!!	ATAAAAAACGATTTTCAATT+AGG	+	chr4_2:56698198-56698217	None:intergenic	15.0%
!!!	TTCAATAAACATTTGTTTTT+TGG	-	chr4_2:56698670-56698689	Msa0609350:intron	15.0%
!!!	TCAATAAACATTTGTTTTTT+GGG	-	chr4_2:56698671-56698690	Msa0609350:intron	15.0%
!!	AGAATGAATGAAATTGAATA+AGG	+	chr4_2:56697913-56697932	None:intergenic	20.0%
!!!	AATTTTCGATTCATGATAAT+TGG	+	chr4_2:56698127-56698146	None:intergenic	20.0%
!!	AATTATCATGAATCGAAAAT+TGG	-	chr4_2:56698126-56698145	Msa0609350:CDS	20.0%
!!!	TGATTTCATATTAGTCTAAA+TGG	-	chr4_2:56698251-56698270	Msa0609350:intron	20.0%
!!!	ACATAACAAATCTAAAATCA+TGG	+	chr4_2:56698364-56698383	None:intergenic	20.0%
!!	CATTTACAATGTCAATAAAT+GGG	+	chr4_2:56698409-56698428	None:intergenic	20.0%
!!	ACATTTACAATGTCAATAAA+TGG	+	chr4_2:56698410-56698429	None:intergenic	20.0%
!!!	CAATAAACATTTGTTTTTTG+GGG	-	chr4_2:56698672-56698691	Msa0609350:intron	20.0%
!!	CAAAATTCAACCAAATAAAA+AGG	+	chr4_2:56698761-56698780	None:intergenic	20.0%
!!	AGAATGAATGAAATTGAATA+AGG	+	chr4_2:56697913-56697932	None:intergenic	20.0%
!!!	AATTTTCGATTCATGATAAT+TGG	+	chr4_2:56698127-56698146	None:intergenic	20.0%
!!	AATTATCATGAATCGAAAAT+TGG	-	chr4_2:56698126-56698145	Msa0609350:CDS	20.0%
!!!	TGATTTCATATTAGTCTAAA+TGG	-	chr4_2:56698251-56698270	Msa0609350:intron	20.0%
!!!	ACATAACAAATCTAAAATCA+TGG	+	chr4_2:56698364-56698383	None:intergenic	20.0%
!!	CATTTACAATGTCAATAAAT+GGG	+	chr4_2:56698409-56698428	None:intergenic	20.0%
!!	ACATTTACAATGTCAATAAA+TGG	+	chr4_2:56698410-56698429	None:intergenic	20.0%
!!!	CAATAAACATTTGTTTTTTG+GGG	-	chr4_2:56698672-56698691	Msa0609350:intron	20.0%
!!	CAAAATTCAACCAAATAAAA+AGG	+	chr4_2:56698761-56698780	None:intergenic	20.0%
!	AATATGAAATCAGTTACACA+TGG	+	chr4_2:56698243-56698262	None:intergenic	25.0%
!	AATATGAAATCAGTTACACA+TGG	+	chr4_2:56698243-56698262	None:intergenic	25.0%
	AAAGCATAAAGCGAAAAGTA+GGG	-	chr4_2:56697789-56697808	Msa0609350:three_prime_UTR	30.0%
!	TTTTTCTCTGCTAGATCTAT+TGG	-	chr4_2:56698162-56698181	Msa0609350:intron	30.0%
	AATGGTATATATCATTCAGC+TGG	+	chr4_2:56698315-56698334	None:intergenic	30.0%
	TAAATGGGAACAATAAGGAA+GGG	+	chr4_2:56698394-56698413	None:intergenic	30.0%
	ATAAATGGGAACAATAAGGA+AGG	+	chr4_2:56698395-56698414	None:intergenic	30.0%
	GTCAATAAATGGGAACAATA+AGG	+	chr4_2:56698399-56698418	None:intergenic	30.0%
	AAAGCATAAAGCGAAAAGTA+GGG	-	chr4_2:56697789-56697808	Msa0609350:three_prime_UTR	30.0%
!	TTTTTCTCTGCTAGATCTAT+TGG	-	chr4_2:56698162-56698181	Msa0609350:intron	30.0%
	AATGGTATATATCATTCAGC+TGG	+	chr4_2:56698315-56698334	None:intergenic	30.0%
	TAAATGGGAACAATAAGGAA+GGG	+	chr4_2:56698394-56698413	None:intergenic	30.0%
	ATAAATGGGAACAATAAGGA+AGG	+	chr4_2:56698395-56698414	None:intergenic	30.0%
	GTCAATAAATGGGAACAATA+AGG	+	chr4_2:56698399-56698418	None:intergenic	30.0%
!	GAAAGCATAAAGCGAAAAGT+AGG	-	chr4_2:56697788-56697807	Msa0609350:three_prime_UTR	35.0%
	GAAATCAGTTACACATGGTT+GGG	+	chr4_2:56698238-56698257	None:intergenic	35.0%
	TGAAATCAGTTACACATGGT+TGG	+	chr4_2:56698239-56698258	None:intergenic	35.0%
!	CTTAAATTTTGCACCTGTTG+TGG	-	chr4_2:56698730-56698749	Msa0609350:intron	35.0%
!	TGTGGCATGACCTTTTTATT+TGG	-	chr4_2:56698748-56698767	Msa0609350:five_prime_UTR	35.0%
!!!	CTTTGACTATCTGCTGTTTT+TGG	-	chr4_2:56698793-56698812	Msa0609350:five_prime_UTR	35.0%
!!!	CTGTTTTTGGATGTAGTTGT+GGG	-	chr4_2:56698806-56698825	Msa0609350:five_prime_UTR	35.0%
!	GAAAGCATAAAGCGAAAAGT+AGG	-	chr4_2:56697788-56697807	Msa0609350:three_prime_UTR	35.0%
	GAAATCAGTTACACATGGTT+GGG	+	chr4_2:56698238-56698257	None:intergenic	35.0%
	TGAAATCAGTTACACATGGT+TGG	+	chr4_2:56698239-56698258	None:intergenic	35.0%
!	CTTAAATTTTGCACCTGTTG+TGG	-	chr4_2:56698730-56698749	Msa0609350:intron	35.0%
!	TGTGGCATGACCTTTTTATT+TGG	-	chr4_2:56698748-56698767	Msa0609350:five_prime_UTR	35.0%
!!!	CTTTGACTATCTGCTGTTTT+TGG	-	chr4_2:56698793-56698812	Msa0609350:five_prime_UTR	35.0%
!!!	CTGTTTTTGGATGTAGTTGT+GGG	-	chr4_2:56698806-56698825	Msa0609350:five_prime_UTR	35.0%
	GATGAAGAAGAAACCTCACT+TGG	+	chr4_2:56697880-56697899	None:intergenic	40.0%
	GGTATATATCATTCAGCTGG+TGG	+	chr4_2:56698312-56698331	None:intergenic	40.0%
	ACTCTCGAATTTCAGCAGAA+TGG	+	chr4_2:56698333-56698352	None:intergenic	40.0%
	CAAGTATGCTGTTGTTGTCA+AGG	-	chr4_2:56698461-56698480	Msa0609350:intron	40.0%
	TGCTGTTGTTGTCAAGGTTA+TGG	-	chr4_2:56698467-56698486	Msa0609350:intron	40.0%
	GCTGTTGTTGTCAAGGTTAT+GGG	-	chr4_2:56698468-56698487	Msa0609350:intron	40.0%
	TCACATTCCTCATGATGTGA+CGG	+	chr4_2:56698554-56698573	None:intergenic	40.0%
	TCACATCATGAGGAATGTGA+AGG	-	chr4_2:56698554-56698573	Msa0609350:intron	40.0%
	CACATCATGAGGAATGTGAA+GGG	-	chr4_2:56698555-56698574	Msa0609350:intron	40.0%
	TTCTCTTTCAGACTCAAGCA+GGG	+	chr4_2:56698607-56698626	None:intergenic	40.0%
	AGTCTGAAAGAGAAGCAAGA+AGG	-	chr4_2:56698613-56698632	Msa0609350:intron	40.0%
	TAAAAAGGTCATGCCACAAC+AGG	+	chr4_2:56698746-56698765	None:intergenic	40.0%
!!!	GCTGTTTTTGGATGTAGTTG+TGG	-	chr4_2:56698805-56698824	Msa0609350:five_prime_UTR	40.0%
	GATGAAGAAGAAACCTCACT+TGG	+	chr4_2:56697880-56697899	None:intergenic	40.0%
	GGTATATATCATTCAGCTGG+TGG	+	chr4_2:56698312-56698331	None:intergenic	40.0%
	ACTCTCGAATTTCAGCAGAA+TGG	+	chr4_2:56698333-56698352	None:intergenic	40.0%
	CAAGTATGCTGTTGTTGTCA+AGG	-	chr4_2:56698461-56698480	Msa0609350:intron	40.0%
	TGCTGTTGTTGTCAAGGTTA+TGG	-	chr4_2:56698467-56698486	Msa0609350:intron	40.0%
	GCTGTTGTTGTCAAGGTTAT+GGG	-	chr4_2:56698468-56698487	Msa0609350:intron	40.0%
	TCACATTCCTCATGATGTGA+CGG	+	chr4_2:56698554-56698573	None:intergenic	40.0%
	TCACATCATGAGGAATGTGA+AGG	-	chr4_2:56698554-56698573	Msa0609350:intron	40.0%
	CACATCATGAGGAATGTGAA+GGG	-	chr4_2:56698555-56698574	Msa0609350:intron	40.0%
	TTCTCTTTCAGACTCAAGCA+GGG	+	chr4_2:56698607-56698626	None:intergenic	40.0%
	AGTCTGAAAGAGAAGCAAGA+AGG	-	chr4_2:56698613-56698632	Msa0609350:intron	40.0%
	TAAAAAGGTCATGCCACAAC+AGG	+	chr4_2:56698746-56698765	None:intergenic	40.0%
!!!	GCTGTTTTTGGATGTAGTTG+TGG	-	chr4_2:56698805-56698824	Msa0609350:five_prime_UTR	40.0%
	ATTGAGTGTTCTTCTTCCCC+CGG	-	chr4_2:56697845-56697864	Msa0609350:three_prime_UTR	45.0%
	GAAGAAACCTCACTTGGTTG+CGG	+	chr4_2:56697874-56697893	None:intergenic	45.0%
	GTGCTTGTGTTTGAGCAGAA+TGG	-	chr4_2:56698428-56698447	Msa0609350:intron	45.0%
	AGGACAGGTGACTCAAGTTA+GGG	-	chr4_2:56698506-56698525	Msa0609350:intron	45.0%
	TTCCTCATGATGTGACGGTT+CGG	+	chr4_2:56698549-56698568	None:intergenic	45.0%
	CTTCTCTTTCAGACTCAAGC+AGG	+	chr4_2:56698608-56698627	None:intergenic	45.0%
	ATTGAGTGTTCTTCTTCCCC+CGG	-	chr4_2:56697845-56697864	Msa0609350:three_prime_UTR	45.0%
	GAAGAAACCTCACTTGGTTG+CGG	+	chr4_2:56697874-56697893	None:intergenic	45.0%
	GTGCTTGTGTTTGAGCAGAA+TGG	-	chr4_2:56698428-56698447	Msa0609350:intron	45.0%
	AGGACAGGTGACTCAAGTTA+GGG	-	chr4_2:56698506-56698525	Msa0609350:intron	45.0%
	TTCCTCATGATGTGACGGTT+CGG	+	chr4_2:56698549-56698568	None:intergenic	45.0%
	CTTCTCTTTCAGACTCAAGC+AGG	+	chr4_2:56698608-56698627	None:intergenic	45.0%
!!	AATTATACTTAATAAAAATT+CGG	+	chr4_2:56698083-56698102	None:intergenic	5.0%
!!	AATTATACTTAATAAAAATT+CGG	+	chr4_2:56698083-56698102	None:intergenic	5.0%
	CTCAATCAAGCTCTGCTCTG+CGG	+	chr4_2:56697831-56697850	None:intergenic	50.0%
	GTCAAGGTTATGGGTCGTAC+TGG	-	chr4_2:56698477-56698496	Msa0609350:intron	50.0%
!!	ATGGGTCGTACTGGTTCTAG+AGG	-	chr4_2:56698486-56698505	Msa0609350:intron	50.0%
!!	TCGTACTGGTTCTAGAGGAC+AGG	-	chr4_2:56698491-56698510	Msa0609350:intron	50.0%
	GAGGACAGGTGACTCAAGTT+AGG	-	chr4_2:56698505-56698524	Msa0609350:intron	50.0%
	TCCTCATGATGTGACGGTTC+GGG	+	chr4_2:56698548-56698567	None:intergenic	50.0%
	GGGTGTCTCCTTCTCTAACT+GGG	+	chr4_2:56698581-56698600	None:intergenic	50.0%
!	AGGGTGTCTCCTTCTCTAAC+TGG	+	chr4_2:56698582-56698601	None:intergenic	50.0%
	TTCAGACTCAAGCAGGGTGA+GGG	+	chr4_2:56698601-56698620	None:intergenic	50.0%
	TTTCAGACTCAAGCAGGGTG+AGG	+	chr4_2:56698602-56698621	None:intergenic	50.0%
	GTTGCGCTAGAGAAACTCAG+CGG	-	chr4_2:56698635-56698654	Msa0609350:intron	50.0%
	GCTAGAGAAACTCAGCGGTT+TGG	-	chr4_2:56698640-56698659	Msa0609350:intron	50.0%
	CTCAATCAAGCTCTGCTCTG+CGG	+	chr4_2:56697831-56697850	None:intergenic	50.0%
	GTCAAGGTTATGGGTCGTAC+TGG	-	chr4_2:56698477-56698496	Msa0609350:intron	50.0%
!!	ATGGGTCGTACTGGTTCTAG+AGG	-	chr4_2:56698486-56698505	Msa0609350:intron	50.0%
!!	TCGTACTGGTTCTAGAGGAC+AGG	-	chr4_2:56698491-56698510	Msa0609350:intron	50.0%
	GAGGACAGGTGACTCAAGTT+AGG	-	chr4_2:56698505-56698524	Msa0609350:intron	50.0%
	TCCTCATGATGTGACGGTTC+GGG	+	chr4_2:56698548-56698567	None:intergenic	50.0%
	GGGTGTCTCCTTCTCTAACT+GGG	+	chr4_2:56698581-56698600	None:intergenic	50.0%
!	AGGGTGTCTCCTTCTCTAAC+TGG	+	chr4_2:56698582-56698601	None:intergenic	50.0%
	TTCAGACTCAAGCAGGGTGA+GGG	+	chr4_2:56698601-56698620	None:intergenic	50.0%
	TTTCAGACTCAAGCAGGGTG+AGG	+	chr4_2:56698602-56698621	None:intergenic	50.0%
	GTTGCGCTAGAGAAACTCAG+CGG	-	chr4_2:56698635-56698654	Msa0609350:intron	50.0%
	GCTAGAGAAACTCAGCGGTT+TGG	-	chr4_2:56698640-56698659	Msa0609350:intron	50.0%
	GAAACCTCACTTGGTTGCGG+TGG	+	chr4_2:56697871-56697890	None:intergenic	55.0%
	ACCCGAACCGTCACATCATG+AGG	-	chr4_2:56698544-56698563	Msa0609350:intron	55.0%
	GTGAAGGGCCCAGTTAGAGA+AGG	-	chr4_2:56698570-56698589	Msa0609350:intron	55.0%
!!	GAAACTCAGCGGTTTGGTGC+AGG	-	chr4_2:56698646-56698665	Msa0609350:intron	55.0%
	GAAACCTCACTTGGTTGCGG+TGG	+	chr4_2:56697871-56697890	None:intergenic	55.0%
	ACCCGAACCGTCACATCATG+AGG	-	chr4_2:56698544-56698563	Msa0609350:intron	55.0%
	GTGAAGGGCCCAGTTAGAGA+AGG	-	chr4_2:56698570-56698589	Msa0609350:intron	55.0%
!!	GAAACTCAGCGGTTTGGTGC+AGG	-	chr4_2:56698646-56698665	Msa0609350:intron	55.0%
	TCACTTGGTTGCGGTGGCCG+GGG	+	chr4_2:56697865-56697884	None:intergenic	65.0%
	CTCACTTGGTTGCGGTGGCC+GGG	+	chr4_2:56697866-56697885	None:intergenic	65.0%
	CCTCACTTGGTTGCGGTGGC+CGG	+	chr4_2:56697867-56697886	None:intergenic	65.0%
	TCACTTGGTTGCGGTGGCCG+GGG	+	chr4_2:56697865-56697884	None:intergenic	65.0%
	CTCACTTGGTTGCGGTGGCC+GGG	+	chr4_2:56697866-56697885	None:intergenic	65.0%
	CCTCACTTGGTTGCGGTGGC+CGG	+	chr4_2:56697867-56697886	None:intergenic	65.0%
	CACTTGGTTGCGGTGGCCGG+GGG	+	chr4_2:56697864-56697883	None:intergenic	70.0%
	CCGGCCACCGCAACCAAGTG+AGG	-	chr4_2:56697864-56697883	Msa0609350:three_prime_UTR	70.0%
	CACTTGGTTGCGGTGGCCGG+GGG	+	chr4_2:56697864-56697883	None:intergenic	70.0%
	CCGGCCACCGCAACCAAGTG+AGG	-	chr4_2:56697864-56697883	Msa0609350:three_prime_UTR	70.0%

Chromosome	Type	Strat	End	Strand	Name
chr4_2	gene	56697767	56698857	56697767	ID=Msa0609350;Name=Msa0609350
chr4_2	mRNA	56697767	56698857	56697767	ID=Msa0609350-mRNA-1;Parent=Msa0609350;Name=Msa0609350-mRNA-1;_AED=0.00;_eAED=0.00;_QI=114\|0\|0.5\|1\|0\|0.5\|2\|213\|65
chr4_2	exon	56697767	56698177	56697767	ID=Msa0609350-mRNA-1:exon:14686;Parent=Msa0609350-mRNA-1
chr4_2	exon	56698744	56698857	56698744	ID=Msa0609350-mRNA-1:exon:14685;Parent=Msa0609350-mRNA-1
chr4_2	five_prime_UTR	56698744	56698857	56698744	ID=Msa0609350-mRNA-1:five_prime_utr;Parent=Msa0609350-mRNA-1
chr4_2	CDS	56697980	56698177	56697980	ID=Msa0609350-mRNA-1:cds;Parent=Msa0609350-mRNA-1
chr4_2	three_prime_UTR	56697767	56697979	56697767	ID=Msa0609350-mRNA-1:three_prime_utr;Parent=Msa0609350-mRNA-1

Gene Sequence	>Msa0609350 ATGGATTCTCAAGTCAAGTATGCTGTTGTTGTCAAGGTTATGGGTCGTACTGGTTCTAGAGGACAGGTGACTCAAGTTAGGGTCAAGTTTAATGACGACCCGAACCGTCACATCATGAGGAATGTGAAGGGCCCAGTTAGAGAAGGAGACACCCTCACCCTGCTTGAGTCTGAAAGAGAAGCAAGAAGGTTGCGCTAG
Protein sequence	>Msa0609350 MDSQVKYAVVVKVMGRTGSRGQVTQVRVKFNDDPNRHIMRNVKGPVREGDTLTLLESEREARRLR*