AlfalfaGEDB | Msa0649890

M. sativa cultivar zhongmu-4 / Msa0649890

KEGG: K02493|K02979|K07767|K08767
GO: GO:0000028 GO:0002181 GO:0003674 GO:0003676 GO:0003723 GO:0003729 GO:0003735 GO:0005198 GO:0005488 GO:0005575 GO:0005618 GO:0005622 GO:0005623 GO:0005737 GO:0005783 GO:0005789 GO:0005791 GO:0005829 GO:0005840 GO:0005844 GO:0005886 GO:0006139 GO:0006364 GO:0006396 GO:0006412 GO:0006518 GO:0006725 GO:0006807 GO:0006996 GO:0007275 GO:0007444 GO:0008150 GO:0008152 GO:0009058 GO:0009059 GO:0009888 GO:0009987 GO:0010467 GO:0012505 GO:0015935 GO:0016020 GO:0016043 GO:0016070 GO:0016072 GO:0019538 GO:0022607 GO:0022613 GO:0022618 GO:0022626 GO:0022627 GO:0030312 GO:0030490 GO:0030867 GO:0031090 GO:0031984 GO:0032501 GO:0032502 GO:0032991 GO:0034470 GO:0034622 GO:0034641 GO:0034645 GO:0034660 GO:0035220 GO:0035295 GO:0042175 GO:0042254 GO:0042255 GO:0042274 GO:0042788 GO:0043043 GO:0043170 GO:0043226 GO:0043227 GO:0043228 GO:0043229 GO:0043231 GO:0043232 GO:0043603 GO:0043604 GO:0043933 GO:0044085 GO:0044237 GO:0044238 GO:0044249 GO:0044260 GO:0044267 GO:0044271 GO:0044391 GO:0044422 GO:0044424 GO:0044425 GO:0044432 GO:0044444 GO:0044445 GO:0044446 GO:0044464 GO:0046483 GO:0048513 GO:0048731 GO:0048856 GO:0060429 GO:0065003 GO:0070925 GO:0071704 GO:0071826 GO:0071840 GO:0071944 GO:0090304 GO:0097159 GO:0098552 GO:0098554 GO:0098556 GO:0098562 GO:0098588 GO:0098827 GO:1901360 GO:1901363 GO:1901564 GO:1901566 GO:1901576 GO:1990904
PFAM: PF01200.21

Nr:

Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa0649890	XP_024637297.1	93.846	65	4	0	1	65	1	65	2.34e-35	124

Swissprot:

Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa0649890	sp\|P46302\|RS28_MAIZE	89.231	65	7	0	1	65	1	65	6.92e-34	112

Trembl:

Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score

TFs/TRs:

Gene ID	Type	Classification

Protein Kinases:

Gene ID	Type	Classification

Network:

Co-expression Network

Gene1	Gene2	correlation coefficient	p_value	FDR
Msa0003330	Msa0649890	0.847194	1.349340e-59	-8.615850e-47
Msa0027590	Msa0649890	0.833336	5.654407e-56	-8.615850e-47
Msa0079560	Msa0649890	0.802527	5.377494e-49	-8.615850e-47
Msa0087910	Msa0649890	0.830407	2.989154e-55	-8.615850e-47
Msa0602900	Msa0649890	0.827461	1.544959e-54	-8.615850e-47
Msa0605890	Msa0649890	0.845067	5.117875e-59	-8.615850e-47
Msa0609350	Msa0649890	0.848788	4.901541e-60	-8.615850e-47
Msa0619230	Msa0649890	0.850914	1.247358e-60	-8.615850e-47
Msa0643960	Msa0649890	0.824784	6.689319e-54	-8.615850e-47
Msa0649890	Msa0656450	0.808056	3.735185e-50	-8.615850e-47
Msa0649890	Msa0686550	0.833337	5.650321e-56	-8.615850e-47
Msa0649890	Msa0694400	0.876009	1.962572e-68	-8.615850e-47
Msa0649890	Msa0704860	0.814224	1.718034e-51	-8.615850e-47
Msa0649890	Msa0708630	0.801765	7.714781e-49	-8.615850e-47
Msa0649890	Msa0788380	0.806855	6.717330e-50	-8.615850e-47
Msa0649890	Msa0902730	0.832658	8.334847e-56	-8.615850e-47
Msa0649890	Msa0929700	0.855471	6.170468e-62	-8.615850e-47
Msa0649890	Msa0930890	0.853438	2.389183e-61	-8.615850e-47
Msa0649890	Msa0978470	0.803280	3.758157e-49	-8.615850e-47
Msa0649890	Msa1037290	0.815411	9.371033e-52	-8.615850e-47
Msa0649890	Msa1080390	0.811861	5.664408e-51	-8.615850e-47
Msa0649890	Msa1082170	0.801840	7.448484e-49	-8.615850e-47
Msa0649890	Msa1093500	0.837487	5.049032e-57	-8.615850e-47
Msa0649890	Msa1125900	0.831001	2.137602e-55	-8.615850e-47
Msa0649890	Msa1126100	0.815544	8.757065e-52	-8.615850e-47
Msa0649890	Msa1126520	0.845087	5.054126e-59	-8.615850e-47
Msa0649890	Msa1164150	0.845870	3.100424e-59	-8.615850e-47
Msa0649890	Msa1180000	0.852814	3.606975e-61	-8.615850e-47
Msa0649890	Msa1186030	0.804824	1.794070e-49	-8.615850e-47
Msa0649890	Msa1235950	0.822138	2.780381e-53	-8.615850e-47
Msa0649890	Msa1235980	0.831599	1.523370e-55	-8.615850e-47
Msa0649890	Msa1258540	0.841393	4.883733e-58	-8.615850e-47
Msa0649890	Msa1270640	0.851102	1.104292e-60	-8.615850e-47
Msa0649890	Msa1270710	0.851515	8.440353e-61	-8.615850e-47
Msa0649890	Msa1295150	0.804591	2.006696e-49	-8.615850e-47
Msa0649890	Msa1311400	0.805940	1.047053e-49	-8.615850e-47
Msa0649890	Msa1351020	0.859122	5.144482e-63	-8.615850e-47
Msa0649890	Msa1368920	0.817326	3.494994e-52	-8.615850e-47
Msa0649890	Msa1374480	0.846290	2.384052e-59	-8.615850e-47
Msa0649890	Msa1385480	0.869622	2.696734e-66	-8.615850e-47
Msa0649890	Msa1390760	0.848228	7.006398e-60	-8.615850e-47
Msa0649890	Msa1407550	0.847303	1.259099e-59	-8.615850e-47
Msa0649890	Msa1413570	0.814391	1.577679e-51	-8.615850e-47
Msa0649890	Msa1432310	0.851972	6.262791e-61	-8.615850e-47
Msa0649890	Msa1446410	0.867372	1.437117e-65	-8.615850e-47
Msa0649890	Msa1463570	0.899124	2.703464e-77	-8.615850e-47
Msa0649890	Msa1465700	0.827577	1.449153e-54	-8.615850e-47
Msa0128330	Msa0649890	0.893741	4.739137e-75	-8.615850e-47
Msa0128750	Msa0649890	0.817089	3.950912e-52	-8.615850e-47
Msa0132460	Msa0649890	0.801638	8.192646e-49	-8.615850e-47
Msa0134580	Msa0649890	0.830108	3.535528e-55	-8.615850e-47
Msa0167860	Msa0649890	0.893902	4.076316e-75	-8.615850e-47
Msa0168320	Msa0649890	0.809784	1.594323e-50	-8.615850e-47
Msa0174580	Msa0649890	0.897115	1.922146e-76	-8.615850e-47
Msa0182050	Msa0649890	0.806600	7.602148e-50	-8.615850e-47
Msa0182100	Msa0649890	0.811954	5.405746e-51	-8.615850e-47
Msa0191450	Msa0649890	0.864066	1.588907e-64	-8.615850e-47
Msa0191550	Msa0649890	0.863984	1.684964e-64	-8.615850e-47
Msa0192300	Msa0649890	0.817025	4.084414e-52	-8.615850e-47
Msa0194940	Msa0649890	0.867422	1.384755e-65	-8.615850e-47
Msa0233550	Msa0649890	0.831320	1.784996e-55	-8.615850e-47
Msa0234850	Msa0649890	0.813105	3.028316e-51	-8.615850e-47
Msa0235050	Msa0649890	0.803555	3.296716e-49	-8.615850e-47
Msa0369410	Msa0649890	0.803220	3.868030e-49	-8.615850e-47
Msa0426090	Msa0649890	0.827577	1.449153e-54	-8.615850e-47
Msa0426250	Msa0649890	0.843950	1.022495e-58	-8.615850e-47
Msa0436750	Msa0649890	0.828441	8.977313e-55	-8.615850e-47
Msa0438960	Msa0649890	0.803602	3.222799e-49	-8.615850e-47
Msa0450150	Msa0649890	0.807083	6.011403e-50	-8.615850e-47
Msa0472480	Msa0649890	0.817908	2.583783e-52	-8.615850e-47
Msa0473570	Msa0649890	0.823073	1.685668e-53	-8.615850e-47
Msa0481520	Msa0649890	0.866058	3.762562e-65	-8.615850e-47
Msa0481800	Msa0649890	0.835954	1.241692e-56	-8.615850e-47
Msa0239420	Msa0649890	0.802807	4.708603e-49	-8.615850e-47
Msa0277600	Msa0649890	0.864907	8.672620e-65	-8.615850e-47
Msa0281370	Msa0649890	0.815201	1.043626e-51	-8.615850e-47
Msa0301250	Msa0649890	0.828219	1.015344e-54	-8.615850e-47
Msa0313060	Msa0649890	0.822337	2.499959e-53	-8.615850e-47
Msa0313520	Msa0649890	0.815791	7.715450e-52	-8.615850e-47
Msa0315430	Msa0649890	0.899060	2.880287e-77	-8.615850e-47
Msa0484730	Msa0649890	0.839036	2.014071e-57	-8.615850e-47
Msa0511760	Msa0649890	0.802712	4.924834e-49	-8.615850e-47
Msa0517480	Msa0649890	0.800528	1.381313e-48	-8.615850e-47
Msa0529900	Msa0649890	0.839686	1.365750e-57	-8.615850e-47
Msa0547920	Msa0649890	0.842398	2.649988e-58	-8.615850e-47
Msa0573550	Msa0649890	0.853339	2.551516e-61	-8.615850e-47
Msa0574780	Msa0649890	0.862996	3.410073e-64	-8.615850e-47
Msa0579830	Msa0649890	0.979632	2.137843e-148	-8.615850e-47
Msa0597240	Msa0649890	0.817483	3.221049e-52	-8.615850e-47

PPI

Gene1	Gene2	Type

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa0649890	MtrunA17_Chr4g0041861	93.846	65	4	0	1	65	1	65	2.15e-39	124
Msa0649890	MtrunA17_Chr6g0453811	90.769	65	6	0	1	65	1	65	1.27e-37	119
Msa0649890	MtrunA17_Chr6g0453911	75.385	65	15	1	1	65	1	64	1.26e-28	96.7
Msa0649890	MtrunA17_Chr8g0381481	78.846	52	11	0	1	52	1	52	1.36e-24	86.3

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa0649890	AT5G03850.1	87.692	65	7	1	1	65	1	64	2.67e-34	111
Msa0649890	AT3G10090.1	87.692	65	7	1	1	65	1	64	2.67e-34	111
Msa0649890	AT5G64140.1	87.692	65	7	1	1	65	1	64	5.23e-34	110

Find 39 sgRNAs with CRISPR-Local

Find 142 sgRNAs with CRISPR-GE

CRISPR-Local

CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
AGGGTGTCTCCTTCTCTAAC+TGG	0.224700	4_3:+56614505	None:intergenic
CCTCACTTGGTTGCGGTGGC+CGG	0.305223	4_3:+56615220	None:intergenic
TGCTGTTGTTGTCAAGGTTA+TGG	0.305748	4_3:-56614617	Msa0649890:CDS
CTCACTTGGTTGCGGTGGCC+GGG	0.328965	4_3:+56615221	None:intergenic
GCTGTTGTTGTCAAGGTTAT+GGG	0.344081	4_3:-56614616	Msa0649890:CDS
GAGGACAGGTGACTCAAGTT+AGG	0.354183	4_3:-56614579	Msa0649890:CDS
TTCCTCATGATGTGACGGTT+CGG	0.361521	4_3:+56614538	None:intergenic
CAAAATTCAACCAAATAAAA+AGG	0.363873	4_3:+56614310	None:intergenic
TCCTCATGATGTGACGGTTC+GGG	0.372907	4_3:+56614539	None:intergenic
CTTCTCTTTCAGACTCAAGC+AGG	0.393026	4_3:+56614479	None:intergenic
TCACTTGGTTGCGGTGGCCG+GGG	0.420954	4_3:+56615222	None:intergenic
GAAAGCATAAAGCGAAAAGT+AGG	0.435439	4_3:-56615296	Msa0649890:five_prime_UTR
GTGCAGGATTGGTCTGGTGC+AGG	0.461885	4_3:-56614422	Msa0649890:three_prime_UTR
GCTAGAGAAACTCAGCGGTT+TGG	0.474314	4_3:-56614444	Msa0649890:exon
GAAACTCAGCGGTTTGGTGC+AGG	0.476171	4_3:-56614438	Msa0649890:three_prime_UTR
TCAGCGGTTTGGTGCAGGAT+TGG	0.481241	4_3:-56614433	Msa0649890:three_prime_UTR
GTCAAGGTTATGGGTCGTAC+TGG	0.486004	4_3:-56614607	Msa0649890:CDS
ATTGAGTGTTCTTCTTCCCC+CGG	0.494777	4_3:-56615239	Msa0649890:five_prime_UTR
TTTCAGACTCAAGCAGGGTG+AGG	0.496062	4_3:+56614485	None:intergenic
CACTTGGTTGCGGTGGCCGG+GGG	0.496936	4_3:+56615223	None:intergenic
GGGTGTCTCCTTCTCTAACT+GGG	0.522501	4_3:+56614506	None:intergenic
AGGACAGGTGACTCAAGTTA+GGG	0.535175	4_3:-56614578	Msa0649890:CDS
CAAGTATGCTGTTGTTGTCA+AGG	0.536125	4_3:-56614623	Msa0649890:CDS
GGTTTGGTGCAGGATTGGTC+TGG	0.538529	4_3:-56614428	Msa0649890:three_prime_UTR
TCGTACTGGTTCTAGAGGAC+AGG	0.538891	4_3:-56614593	Msa0649890:CDS
TTCAGACTCAAGCAGGGTGA+GGG	0.547860	4_3:+56614486	None:intergenic
ACCCGAACCGTCACATCATG+AGG	0.555528	4_3:-56614540	Msa0649890:CDS
AAAGCATAAAGCGAAAAGTA+GGG	0.572772	4_3:-56615295	Msa0649890:five_prime_UTR
GAAGAAACCTCACTTGGTTG+CGG	0.577435	4_3:+56615213	None:intergenic
GAAACCTCACTTGGTTGCGG+TGG	0.582952	4_3:+56615216	None:intergenic
TTCTCTTTCAGACTCAAGCA+GGG	0.585301	4_3:+56614480	None:intergenic
TCACATCATGAGGAATGTGA+AGG	0.587194	4_3:-56614530	Msa0649890:CDS
TCACATTCCTCATGATGTGA+CGG	0.589714	4_3:+56614533	None:intergenic
CACATCATGAGGAATGTGAA+GGG	0.593653	4_3:-56614529	Msa0649890:CDS
GTGAAGGGCCCAGTTAGAGA+AGG	0.598823	4_3:-56614514	Msa0649890:CDS
AGTCTGAAAGAGAAGCAAGA+AGG	0.599710	4_3:-56614471	Msa0649890:CDS
ATGGGTCGTACTGGTTCTAG+AGG	0.630812	4_3:-56614598	Msa0649890:CDS
CTCAATCAAGCTCTGCTCTG+CGG	0.700611	4_3:+56615256	None:intergenic
GTTGCGCTAGAGAAACTCAG+CGG	0.778395	4_3:-56614449	Msa0649890:exon

CRISPR-GE

badsite warning	sgRNA_sequence	Strand	Position	Region	GC_content
!!	TTAAAAAAAACAAAGAAAAA+AGG	+	chr4_3:56614430-56614449	None:intergenic	10.0%
!!	TTAAAAAAAACAAAGAAAAA+AGG	+	chr4_3:56614430-56614449	None:intergenic	10.0%
!!!	ATAAAAAACGATTTTCAATT+AGG	+	chr4_3:56614655-56614674	None:intergenic	15.0%
!!!	TTCAATAAACATTTGTTTTT+TGG	-	chr4_3:56615143-56615162	Msa0649890:intron	15.0%
!!!	TCAATAAACATTTGTTTTTT+GGG	-	chr4_3:56615144-56615163	Msa0649890:intron	15.0%
!!!	ATAAAAAACGATTTTCAATT+AGG	+	chr4_3:56614655-56614674	None:intergenic	15.0%
!!!	TTCAATAAACATTTGTTTTT+TGG	-	chr4_3:56615143-56615162	Msa0649890:intron	15.0%
!!!	TCAATAAACATTTGTTTTTT+GGG	-	chr4_3:56615144-56615163	Msa0649890:intron	15.0%
!!	AGAATGAATGAAATTGAATA+AGG	+	chr4_3:56614370-56614389	None:intergenic	20.0%
!!!	AATTTTCGATTCATGATAAT+TGG	+	chr4_3:56614584-56614603	None:intergenic	20.0%
!!	AATTATCATGAATCGAAAAT+TGG	-	chr4_3:56614583-56614602	Msa0649890:CDS	20.0%
!!!	TGATTTCATATTAGTCTAAA+TGG	-	chr4_3:56614708-56614727	Msa0649890:intron	20.0%
!!!	ACATAACAAATCTAAAATCA+TGG	+	chr4_3:56614821-56614840	None:intergenic	20.0%
!!	CATTTACAATGTCAATAAAT+GGG	+	chr4_3:56614866-56614885	None:intergenic	20.0%
!!	ACATTTACAATGTCAATAAA+TGG	+	chr4_3:56614867-56614886	None:intergenic	20.0%
!!!	CAATAAACATTTGTTTTTTG+GGG	-	chr4_3:56615145-56615164	Msa0649890:intron	20.0%
!!	CAAAATTCAACCAAATAAAA+AGG	+	chr4_3:56615234-56615253	None:intergenic	20.0%
!!	AGAATGAATGAAATTGAATA+AGG	+	chr4_3:56614370-56614389	None:intergenic	20.0%
!!!	AATTTTCGATTCATGATAAT+TGG	+	chr4_3:56614584-56614603	None:intergenic	20.0%
!!	AATTATCATGAATCGAAAAT+TGG	-	chr4_3:56614583-56614602	Msa0649890:CDS	20.0%
!!!	TGATTTCATATTAGTCTAAA+TGG	-	chr4_3:56614708-56614727	Msa0649890:intron	20.0%
!!!	ACATAACAAATCTAAAATCA+TGG	+	chr4_3:56614821-56614840	None:intergenic	20.0%
!!	CATTTACAATGTCAATAAAT+GGG	+	chr4_3:56614866-56614885	None:intergenic	20.0%
!!	ACATTTACAATGTCAATAAA+TGG	+	chr4_3:56614867-56614886	None:intergenic	20.0%
!!!	CAATAAACATTTGTTTTTTG+GGG	-	chr4_3:56615145-56615164	Msa0649890:intron	20.0%
!!	CAAAATTCAACCAAATAAAA+AGG	+	chr4_3:56615234-56615253	None:intergenic	20.0%
!	AATATGAAATCAGTTACACA+TGG	+	chr4_3:56614700-56614719	None:intergenic	25.0%
!	AATATGAAATCAGTTACACA+TGG	+	chr4_3:56614700-56614719	None:intergenic	25.0%
	AAAGCATAAAGCGAAAAGTA+GGG	-	chr4_3:56614246-56614265	Msa0649890:three_prime_UTR	30.0%
!	TTTTTCTCTGCTAGATCTAT+TGG	-	chr4_3:56614619-56614638	Msa0649890:CDS	30.0%
	AATGGTATATATCATTCAGC+TGG	+	chr4_3:56614772-56614791	None:intergenic	30.0%
	TAAATGGGAACAATAAGGAA+GGG	+	chr4_3:56614851-56614870	None:intergenic	30.0%
	ATAAATGGGAACAATAAGGA+AGG	+	chr4_3:56614852-56614871	None:intergenic	30.0%
	GTCAATAAATGGGAACAATA+AGG	+	chr4_3:56614856-56614875	None:intergenic	30.0%
	AAAGCATAAAGCGAAAAGTA+GGG	-	chr4_3:56614246-56614265	Msa0649890:three_prime_UTR	30.0%
!	TTTTTCTCTGCTAGATCTAT+TGG	-	chr4_3:56614619-56614638	Msa0649890:CDS	30.0%
	AATGGTATATATCATTCAGC+TGG	+	chr4_3:56614772-56614791	None:intergenic	30.0%
	TAAATGGGAACAATAAGGAA+GGG	+	chr4_3:56614851-56614870	None:intergenic	30.0%
	ATAAATGGGAACAATAAGGA+AGG	+	chr4_3:56614852-56614871	None:intergenic	30.0%
	GTCAATAAATGGGAACAATA+AGG	+	chr4_3:56614856-56614875	None:intergenic	30.0%
!	GAAAGCATAAAGCGAAAAGT+AGG	-	chr4_3:56614245-56614264	Msa0649890:three_prime_UTR	35.0%
	GAAATCAGTTACACATGGTT+GGG	+	chr4_3:56614695-56614714	None:intergenic	35.0%
	TGAAATCAGTTACACATGGT+TGG	+	chr4_3:56614696-56614715	None:intergenic	35.0%
!	CTTAAATTTTGCACCTGTTG+TGG	-	chr4_3:56615203-56615222	Msa0649890:intron	35.0%
!	TGTGGCATGACCTTTTTATT+TGG	-	chr4_3:56615221-56615240	Msa0649890:intron	35.0%
!!!	CTTTGACTATCTGCTGTTTT+TGG	-	chr4_3:56615266-56615285	Msa0649890:five_prime_UTR	35.0%
!!!	CTGTTTTTGGATGTAGTTGT+GGG	-	chr4_3:56615279-56615298	Msa0649890:five_prime_UTR	35.0%
!	GAAAGCATAAAGCGAAAAGT+AGG	-	chr4_3:56614245-56614264	Msa0649890:three_prime_UTR	35.0%
	GAAATCAGTTACACATGGTT+GGG	+	chr4_3:56614695-56614714	None:intergenic	35.0%
	TGAAATCAGTTACACATGGT+TGG	+	chr4_3:56614696-56614715	None:intergenic	35.0%
!	CTTAAATTTTGCACCTGTTG+TGG	-	chr4_3:56615203-56615222	Msa0649890:intron	35.0%
!	TGTGGCATGACCTTTTTATT+TGG	-	chr4_3:56615221-56615240	Msa0649890:intron	35.0%
!!!	CTTTGACTATCTGCTGTTTT+TGG	-	chr4_3:56615266-56615285	Msa0649890:five_prime_UTR	35.0%
!!!	CTGTTTTTGGATGTAGTTGT+GGG	-	chr4_3:56615279-56615298	Msa0649890:five_prime_UTR	35.0%
	GATGAAGAAGAAACCTCACT+TGG	+	chr4_3:56614337-56614356	None:intergenic	40.0%
	GGTATATATCATTCAGCTGG+TGG	+	chr4_3:56614769-56614788	None:intergenic	40.0%
	ACTCTCGAATTTCAGCAGAA+TGG	+	chr4_3:56614790-56614809	None:intergenic	40.0%
	CAAGTATGCTGTTGTTGTCA+AGG	-	chr4_3:56614918-56614937	Msa0649890:intron	40.0%
	TGCTGTTGTTGTCAAGGTTA+TGG	-	chr4_3:56614924-56614943	Msa0649890:intron	40.0%
	GCTGTTGTTGTCAAGGTTAT+GGG	-	chr4_3:56614925-56614944	Msa0649890:intron	40.0%
	TCACATTCCTCATGATGTGA+CGG	+	chr4_3:56615011-56615030	None:intergenic	40.0%
	TCACATCATGAGGAATGTGA+AGG	-	chr4_3:56615011-56615030	Msa0649890:intron	40.0%
	CACATCATGAGGAATGTGAA+GGG	-	chr4_3:56615012-56615031	Msa0649890:intron	40.0%
	TTCTCTTTCAGACTCAAGCA+GGG	+	chr4_3:56615064-56615083	None:intergenic	40.0%
	AGTCTGAAAGAGAAGCAAGA+AGG	-	chr4_3:56615070-56615089	Msa0649890:intron	40.0%
	TAAAAAGGTCATGCCACAAC+AGG	+	chr4_3:56615219-56615238	None:intergenic	40.0%
!!!	GCTGTTTTTGGATGTAGTTG+TGG	-	chr4_3:56615278-56615297	Msa0649890:five_prime_UTR	40.0%
	GATGAAGAAGAAACCTCACT+TGG	+	chr4_3:56614337-56614356	None:intergenic	40.0%
	GGTATATATCATTCAGCTGG+TGG	+	chr4_3:56614769-56614788	None:intergenic	40.0%
	ACTCTCGAATTTCAGCAGAA+TGG	+	chr4_3:56614790-56614809	None:intergenic	40.0%
	CAAGTATGCTGTTGTTGTCA+AGG	-	chr4_3:56614918-56614937	Msa0649890:intron	40.0%
	TGCTGTTGTTGTCAAGGTTA+TGG	-	chr4_3:56614924-56614943	Msa0649890:intron	40.0%
	GCTGTTGTTGTCAAGGTTAT+GGG	-	chr4_3:56614925-56614944	Msa0649890:intron	40.0%
	TCACATTCCTCATGATGTGA+CGG	+	chr4_3:56615011-56615030	None:intergenic	40.0%
	TCACATCATGAGGAATGTGA+AGG	-	chr4_3:56615011-56615030	Msa0649890:intron	40.0%
	CACATCATGAGGAATGTGAA+GGG	-	chr4_3:56615012-56615031	Msa0649890:intron	40.0%
	TTCTCTTTCAGACTCAAGCA+GGG	+	chr4_3:56615064-56615083	None:intergenic	40.0%
	AGTCTGAAAGAGAAGCAAGA+AGG	-	chr4_3:56615070-56615089	Msa0649890:intron	40.0%
	TAAAAAGGTCATGCCACAAC+AGG	+	chr4_3:56615219-56615238	None:intergenic	40.0%
!!!	GCTGTTTTTGGATGTAGTTG+TGG	-	chr4_3:56615278-56615297	Msa0649890:five_prime_UTR	40.0%
	ATTGAGTGTTCTTCTTCCCC+CGG	-	chr4_3:56614302-56614321	Msa0649890:three_prime_UTR	45.0%
	GAAGAAACCTCACTTGGTTG+CGG	+	chr4_3:56614331-56614350	None:intergenic	45.0%
	GTGCTTGTGTTTGAGCAGAA+TGG	-	chr4_3:56614885-56614904	Msa0649890:intron	45.0%
	AGGACAGGTGACTCAAGTTA+GGG	-	chr4_3:56614963-56614982	Msa0649890:intron	45.0%
	TTCCTCATGATGTGACGGTT+CGG	+	chr4_3:56615006-56615025	None:intergenic	45.0%
	CTTCTCTTTCAGACTCAAGC+AGG	+	chr4_3:56615065-56615084	None:intergenic	45.0%
	ATTGAGTGTTCTTCTTCCCC+CGG	-	chr4_3:56614302-56614321	Msa0649890:three_prime_UTR	45.0%
	GAAGAAACCTCACTTGGTTG+CGG	+	chr4_3:56614331-56614350	None:intergenic	45.0%
	GTGCTTGTGTTTGAGCAGAA+TGG	-	chr4_3:56614885-56614904	Msa0649890:intron	45.0%
	AGGACAGGTGACTCAAGTTA+GGG	-	chr4_3:56614963-56614982	Msa0649890:intron	45.0%
	TTCCTCATGATGTGACGGTT+CGG	+	chr4_3:56615006-56615025	None:intergenic	45.0%
	CTTCTCTTTCAGACTCAAGC+AGG	+	chr4_3:56615065-56615084	None:intergenic	45.0%
!!	AATTATACTTAATAAAAATT+CGG	+	chr4_3:56614540-56614559	None:intergenic	5.0%
!!	AATTATACTTAATAAAAATT+CGG	+	chr4_3:56614540-56614559	None:intergenic	5.0%
	CTCAATCAAGCTCTGCTCTG+CGG	+	chr4_3:56614288-56614307	None:intergenic	50.0%
	GTCAAGGTTATGGGTCGTAC+TGG	-	chr4_3:56614934-56614953	Msa0649890:intron	50.0%
!!	ATGGGTCGTACTGGTTCTAG+AGG	-	chr4_3:56614943-56614962	Msa0649890:intron	50.0%
!!	TCGTACTGGTTCTAGAGGAC+AGG	-	chr4_3:56614948-56614967	Msa0649890:intron	50.0%
	GAGGACAGGTGACTCAAGTT+AGG	-	chr4_3:56614962-56614981	Msa0649890:intron	50.0%
	TCCTCATGATGTGACGGTTC+GGG	+	chr4_3:56615005-56615024	None:intergenic	50.0%
	GGGTGTCTCCTTCTCTAACT+GGG	+	chr4_3:56615038-56615057	None:intergenic	50.0%
!	AGGGTGTCTCCTTCTCTAAC+TGG	+	chr4_3:56615039-56615058	None:intergenic	50.0%
	TTCAGACTCAAGCAGGGTGA+GGG	+	chr4_3:56615058-56615077	None:intergenic	50.0%
	TTTCAGACTCAAGCAGGGTG+AGG	+	chr4_3:56615059-56615078	None:intergenic	50.0%
	GTTGCGCTAGAGAAACTCAG+CGG	-	chr4_3:56615092-56615111	Msa0649890:intron	50.0%
	GCTAGAGAAACTCAGCGGTT+TGG	-	chr4_3:56615097-56615116	Msa0649890:intron	50.0%
	CTCAATCAAGCTCTGCTCTG+CGG	+	chr4_3:56614288-56614307	None:intergenic	50.0%
	GTCAAGGTTATGGGTCGTAC+TGG	-	chr4_3:56614934-56614953	Msa0649890:intron	50.0%
!!	ATGGGTCGTACTGGTTCTAG+AGG	-	chr4_3:56614943-56614962	Msa0649890:intron	50.0%
!!	TCGTACTGGTTCTAGAGGAC+AGG	-	chr4_3:56614948-56614967	Msa0649890:intron	50.0%
	GAGGACAGGTGACTCAAGTT+AGG	-	chr4_3:56614962-56614981	Msa0649890:intron	50.0%
	TCCTCATGATGTGACGGTTC+GGG	+	chr4_3:56615005-56615024	None:intergenic	50.0%
	GGGTGTCTCCTTCTCTAACT+GGG	+	chr4_3:56615038-56615057	None:intergenic	50.0%
!	AGGGTGTCTCCTTCTCTAAC+TGG	+	chr4_3:56615039-56615058	None:intergenic	50.0%
	TTCAGACTCAAGCAGGGTGA+GGG	+	chr4_3:56615058-56615077	None:intergenic	50.0%
	TTTCAGACTCAAGCAGGGTG+AGG	+	chr4_3:56615059-56615078	None:intergenic	50.0%
	GTTGCGCTAGAGAAACTCAG+CGG	-	chr4_3:56615092-56615111	Msa0649890:intron	50.0%
	GCTAGAGAAACTCAGCGGTT+TGG	-	chr4_3:56615097-56615116	Msa0649890:intron	50.0%
	GAAACCTCACTTGGTTGCGG+TGG	+	chr4_3:56614328-56614347	None:intergenic	55.0%
	ACCCGAACCGTCACATCATG+AGG	-	chr4_3:56615001-56615020	Msa0649890:intron	55.0%
	GTGAAGGGCCCAGTTAGAGA+AGG	-	chr4_3:56615027-56615046	Msa0649890:intron	55.0%
!!	GAAACTCAGCGGTTTGGTGC+AGG	-	chr4_3:56615103-56615122	Msa0649890:intron	55.0%
!!	TCAGCGGTTTGGTGCAGGAT+TGG	-	chr4_3:56615108-56615127	Msa0649890:intron	55.0%
!!	GGTTTGGTGCAGGATTGGTC+TGG	-	chr4_3:56615113-56615132	Msa0649890:intron	55.0%
	GAAACCTCACTTGGTTGCGG+TGG	+	chr4_3:56614328-56614347	None:intergenic	55.0%
	ACCCGAACCGTCACATCATG+AGG	-	chr4_3:56615001-56615020	Msa0649890:intron	55.0%
	GTGAAGGGCCCAGTTAGAGA+AGG	-	chr4_3:56615027-56615046	Msa0649890:intron	55.0%
!!	GAAACTCAGCGGTTTGGTGC+AGG	-	chr4_3:56615103-56615122	Msa0649890:intron	55.0%
!!	TCAGCGGTTTGGTGCAGGAT+TGG	-	chr4_3:56615108-56615127	Msa0649890:intron	55.0%
!!	GGTTTGGTGCAGGATTGGTC+TGG	-	chr4_3:56615113-56615132	Msa0649890:intron	55.0%
!!	GTGCAGGATTGGTCTGGTGC+AGG	-	chr4_3:56615119-56615138	Msa0649890:intron	60.0%
!!	GTGCAGGATTGGTCTGGTGC+AGG	-	chr4_3:56615119-56615138	Msa0649890:intron	60.0%
	TCACTTGGTTGCGGTGGCCG+GGG	+	chr4_3:56614322-56614341	None:intergenic	65.0%
	CTCACTTGGTTGCGGTGGCC+GGG	+	chr4_3:56614323-56614342	None:intergenic	65.0%
	CCTCACTTGGTTGCGGTGGC+CGG	+	chr4_3:56614324-56614343	None:intergenic	65.0%
	TCACTTGGTTGCGGTGGCCG+GGG	+	chr4_3:56614322-56614341	None:intergenic	65.0%
	CTCACTTGGTTGCGGTGGCC+GGG	+	chr4_3:56614323-56614342	None:intergenic	65.0%
	CCTCACTTGGTTGCGGTGGC+CGG	+	chr4_3:56614324-56614343	None:intergenic	65.0%
	CACTTGGTTGCGGTGGCCGG+GGG	+	chr4_3:56614321-56614340	None:intergenic	70.0%
	CCGGCCACCGCAACCAAGTG+AGG	-	chr4_3:56614321-56614340	Msa0649890:three_prime_UTR	70.0%
	CACTTGGTTGCGGTGGCCGG+GGG	+	chr4_3:56614321-56614340	None:intergenic	70.0%
	CCGGCCACCGCAACCAAGTG+AGG	-	chr4_3:56614321-56614340	Msa0649890:three_prime_UTR	70.0%

Chromosome	Type	Strat	End	Strand	Name
chr4_3	gene	56614224	56615339	56614224	ID=Msa0649890;Name=Msa0649890
chr4_3	mRNA	56614224	56615339	56614224	ID=Msa0649890-mRNA-1;Parent=Msa0649890;Name=Msa0649890-mRNA-1;_AED=0.00;_eAED=0.00;_QI=114\|0\|0.5\|1\|0\|0.5\|2\|238\|65
chr4_3	exon	56614224	56614659	56614224	ID=Msa0649890-mRNA-1:exon:14793;Parent=Msa0649890-mRNA-1
chr4_3	exon	56615226	56615339	56615226	ID=Msa0649890-mRNA-1:exon:14792;Parent=Msa0649890-mRNA-1
chr4_3	five_prime_UTR	56615226	56615339	56615226	ID=Msa0649890-mRNA-1:five_prime_utr;Parent=Msa0649890-mRNA-1
chr4_3	CDS	56614462	56614659	56614462	ID=Msa0649890-mRNA-1:cds;Parent=Msa0649890-mRNA-1
chr4_3	three_prime_UTR	56614224	56614461	56614224	ID=Msa0649890-mRNA-1:three_prime_utr;Parent=Msa0649890-mRNA-1

Gene Sequence	>Msa0649890 ATGGATTCTCAAGTCAAGTATGCTGTTGTTGTCAAGGTTATGGGTCGTACTGGTTCTAGAGGACAGGTGACTCAAGTTAGGGTCAAGTTTAATGACGACCCGAACCGTCACATCATGAGGAATGTGAAGGGCCCAGTTAGAGAAGGAGACACCCTCACCCTGCTTGAGTCTGAAAGAGAAGCAAGAAGGTTGCGCTAG
Protein sequence	>Msa0649890 MDSQVKYAVVVKVMGRTGSRGQVTQVRVKFNDDPNRHIMRNVKGPVREGDTLTLLESEREARRLR*