AlfalfaGEDB | Msa0579830

M. sativa cultivar zhongmu-4 / Msa0579830

KEGG: K02493|K02979|K07767|K08767
GO: GO:0000028 GO:0002181 GO:0003674 GO:0003676 GO:0003723 GO:0003729 GO:0003735 GO:0005198 GO:0005488 GO:0005575 GO:0005618 GO:0005622 GO:0005623 GO:0005737 GO:0005783 GO:0005789 GO:0005791 GO:0005829 GO:0005840 GO:0005844 GO:0005886 GO:0006139 GO:0006364 GO:0006396 GO:0006412 GO:0006518 GO:0006725 GO:0006807 GO:0006996 GO:0007275 GO:0007444 GO:0008150 GO:0008152 GO:0009058 GO:0009059 GO:0009888 GO:0009987 GO:0010467 GO:0012505 GO:0015935 GO:0016020 GO:0016043 GO:0016070 GO:0016072 GO:0019538 GO:0022607 GO:0022613 GO:0022618 GO:0022626 GO:0022627 GO:0030312 GO:0030490 GO:0030867 GO:0031090 GO:0031984 GO:0032501 GO:0032502 GO:0032991 GO:0034470 GO:0034622 GO:0034641 GO:0034645 GO:0034660 GO:0035220 GO:0035295 GO:0042175 GO:0042254 GO:0042255 GO:0042274 GO:0042788 GO:0043043 GO:0043170 GO:0043226 GO:0043227 GO:0043228 GO:0043229 GO:0043231 GO:0043232 GO:0043603 GO:0043604 GO:0043933 GO:0044085 GO:0044237 GO:0044238 GO:0044249 GO:0044260 GO:0044267 GO:0044271 GO:0044391 GO:0044422 GO:0044424 GO:0044425 GO:0044432 GO:0044444 GO:0044445 GO:0044446 GO:0044464 GO:0046483 GO:0048513 GO:0048731 GO:0048856 GO:0060429 GO:0065003 GO:0070925 GO:0071704 GO:0071826 GO:0071840 GO:0071944 GO:0090304 GO:0097159 GO:0098552 GO:0098554 GO:0098556 GO:0098562 GO:0098588 GO:0098827 GO:1901360 GO:1901363 GO:1901564 GO:1901566 GO:1901576 GO:1990904
PFAM: PF01200.21

Nr:

Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa0579830	XP_024637297.1	93.846	65	4	0	1	65	1	65	2.34e-35	124

Swissprot:

Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa0579830	sp\|P46302\|RS28_MAIZE	89.231	65	7	0	1	65	1	65	6.92e-34	112

Trembl:

Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa0579830	A0A396I8I6	93.846	65	4	0	1	65	1	65	1.12e-35	124

TFs/TRs:

Gene ID	Type	Classification

Protein Kinases:

Gene ID	Type	Classification

Network:

Co-expression Network

Gene1	Gene2	correlation coefficient	p_value	FDR
Msa0003330	Msa0579830	0.840394	8.928315e-58	-8.615850e-47
Msa0027590	Msa0579830	0.831158	1.955540e-55	-8.615850e-47
Msa0087910	Msa0579830	0.844986	5.380918e-59	-8.615850e-47
Msa0114600	Msa0579830	0.810233	1.276499e-50	-8.615850e-47
Msa0128330	Msa0579830	0.897615	1.184162e-76	-8.615850e-47
Msa0128750	Msa0579830	0.829768	4.278966e-55	-8.615850e-47
Msa0132460	Msa0579830	0.802416	5.667892e-49	-8.615850e-47
Msa0134580	Msa0579830	0.838410	2.923199e-57	-8.615850e-47
Msa0167860	Msa0579830	0.894148	3.238800e-75	-8.615850e-47
Msa0174580	Msa0579830	0.900091	1.035903e-77	-8.615850e-47
Msa0182050	Msa0579830	0.807298	5.412029e-50	-8.615850e-47
Msa0182100	Msa0579830	0.809513	1.822912e-50	-8.615850e-47
Msa0191450	Msa0579830	0.864462	1.194878e-64	-8.615850e-47
Msa0191550	Msa0579830	0.869004	4.283865e-66	-8.615850e-47
Msa0192300	Msa0579830	0.801711	7.914765e-49	-8.615850e-47
Msa0194940	Msa0579830	0.868621	5.698625e-66	-8.615850e-47
Msa0233550	Msa0579830	0.814906	1.213279e-51	-8.615850e-47
Msa0234850	Msa0579830	0.823725	1.186559e-53	-8.615850e-47
Msa0235050	Msa0579830	0.823024	1.730721e-53	-8.615850e-47
Msa0369410	Msa0579830	0.810050	1.397588e-50	-8.615850e-47
Msa0419710	Msa0579830	0.804371	2.230664e-49	-8.615850e-47
Msa0426090	Msa0579830	0.830754	2.457342e-55	-8.615850e-47
Msa0426250	Msa0579830	0.856207	3.761511e-62	-8.615850e-47
Msa0436750	Msa0579830	0.814985	1.165842e-51	-8.615850e-47
Msa0438960	Msa0579830	0.813385	2.628802e-51	-8.615850e-47
Msa0450150	Msa0579830	0.815871	7.402613e-52	-8.615850e-47
Msa0472480	Msa0579830	0.819157	1.345779e-52	-8.615850e-47
Msa0473570	Msa0579830	0.817546	3.117512e-52	-8.615850e-47
Msa0481520	Msa0579830	0.857786	1.287436e-62	-8.615850e-47
Msa0481800	Msa0579830	0.829834	4.124680e-55	-8.615850e-47
Msa0481850	Msa0579830	0.810115	1.352911e-50	-8.615850e-47
Msa0277600	Msa0579830	0.875122	3.950331e-68	-8.615850e-47
Msa0281370	Msa0579830	0.814699	1.349016e-51	-8.615850e-47
Msa0301250	Msa0579830	0.812799	3.534083e-51	-8.615850e-47
Msa0313060	Msa0579830	0.814249	1.696166e-51	-8.615850e-47
Msa0313520	Msa0579830	0.825733	3.991107e-54	-8.615850e-47
Msa0315430	Msa0579830	0.892072	2.222740e-74	-8.615850e-47
Msa0484730	Msa0579830	0.836336	9.933258e-57	-8.615850e-47
Msa0517480	Msa0579830	0.800585	1.345051e-48	-8.615850e-47
Msa0529900	Msa0579830	0.835675	1.461733e-56	-8.615850e-47
Msa0547920	Msa0579830	0.845283	4.472290e-59	-8.615850e-47
Msa0573550	Msa0579830	0.851635	7.807056e-61	-8.615850e-47
Msa0574780	Msa0579830	0.852442	4.604148e-61	-8.615850e-47
Msa0579830	Msa0597240	0.813851	2.076217e-51	-8.615850e-47
Msa0579830	Msa0602900	0.830075	3.601171e-55	-8.615850e-47
Msa0579830	Msa0605890	0.852733	3.803346e-61	-8.615850e-47
Msa0579830	Msa0609350	0.840064	1.088783e-57	-8.615850e-47
Msa0579830	Msa0619230	0.841058	5.981962e-58	-8.615850e-47
Msa0579830	Msa0643960	0.827952	1.177376e-54	-8.615850e-47
Msa0579830	Msa0649890	0.979632	2.137843e-148	-8.615850e-47
Msa0579830	Msa0686550	0.833505	5.132135e-56	-8.615850e-47
Msa0579830	Msa0694400	0.871766	5.321810e-67	-8.615850e-47
Msa0579830	Msa0704860	0.812272	4.609406e-51	-8.615850e-47
Msa0579830	Msa0708630	0.802739	4.863319e-49	-8.615850e-47
Msa0579830	Msa0741510	0.821022	5.034441e-53	-8.615850e-47
Msa0579830	Msa0749000	0.810832	9.473610e-51	-8.615850e-47
Msa0579830	Msa0788380	0.808641	2.803443e-50	-8.615850e-47
Msa0579830	Msa0796490	0.813149	2.961624e-51	-8.615850e-47
Msa0579830	Msa0902730	0.838545	2.698274e-57	-8.615850e-47
Msa0579830	Msa0929700	0.854174	1.467397e-61	-8.615850e-47
Msa0579830	Msa0930890	0.851405	9.064739e-61	-8.615850e-47
Msa0579830	Msa0978470	0.814652	1.381456e-51	-8.615850e-47
Msa0579830	Msa1037290	0.828016	1.136204e-54	-8.615850e-47
Msa0579830	Msa1080390	0.803932	2.753609e-49	-8.615850e-47
Msa0579830	Msa1082170	0.809370	1.956958e-50	-8.615850e-47
Msa0579830	Msa1093500	0.851720	7.382829e-61	-8.615850e-47
Msa0579830	Msa1125900	0.824356	8.437460e-54	-8.615850e-47
Msa0579830	Msa1126100	0.810171	1.316212e-50	-8.615850e-47
Msa0579830	Msa1126520	0.849730	2.679771e-60	-8.615850e-47
Msa0579830	Msa1164150	0.855298	6.927426e-62	-8.615850e-47
Msa0579830	Msa1180000	0.857286	1.810281e-62	-8.615850e-47
Msa0579830	Msa1186030	0.812637	3.835613e-51	-8.615850e-47
Msa0579830	Msa1235950	0.811522	6.711944e-51	-8.615850e-47
Msa0579830	Msa1235980	0.820257	7.548440e-53	-8.615850e-47
Msa0579830	Msa1240270	0.815027	1.140611e-51	-8.615850e-47
Msa0579830	Msa1258540	0.838310	3.103383e-57	-8.615850e-47
Msa0579830	Msa1270640	0.858055	1.070783e-62	-8.615850e-47
Msa0579830	Msa1270710	0.857030	2.153854e-62	-8.615850e-47
Msa0579830	Msa1295150	0.824524	7.705522e-54	-8.615850e-47
Msa0579830	Msa1311400	0.809574	1.769223e-50	-8.615850e-47
Msa0579830	Msa1351020	0.856325	3.472573e-62	-8.615850e-47
Msa0579830	Msa1368920	0.829609	4.676872e-55	-8.615850e-47
Msa0579830	Msa1374480	0.845568	3.744971e-59	-8.615850e-47
Msa0579830	Msa1385480	0.870325	1.589137e-66	-8.615850e-47
Msa0579830	Msa1390760	0.837682	4.499273e-57	-8.615850e-47
Msa0579830	Msa1407550	0.832160	1.107770e-55	-8.615850e-47
Msa0579830	Msa1432310	0.840223	9.897474e-58	-8.615850e-47
Msa0579830	Msa1446410	0.857259	1.843148e-62	-8.615850e-47
Msa0579830	Msa1463570	0.900833	4.930795e-78	-8.615850e-47
Msa0579830	Msa1465700	0.812833	3.474002e-51	-8.615850e-47

PPI

Gene1	Gene2	Type

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa0579830	MtrunA17_Chr4g0041861	93.846	65	4	0	1	65	1	65	2.15e-39	124
Msa0579830	MtrunA17_Chr6g0453811	90.769	65	6	0	1	65	1	65	1.27e-37	119
Msa0579830	MtrunA17_Chr6g0453911	75.385	65	15	1	1	65	1	64	1.26e-28	96.7
Msa0579830	MtrunA17_Chr8g0381481	78.846	52	11	0	1	52	1	52	1.36e-24	86.3

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa0579830	AT5G03850.1	87.692	65	7	1	1	65	1	64	2.67e-34	111
Msa0579830	AT3G10090.1	87.692	65	7	1	1	65	1	64	2.67e-34	111
Msa0579830	AT5G64140.1	87.692	65	7	1	1	65	1	64	5.23e-34	110

Find 39 sgRNAs with CRISPR-Local

Find 142 sgRNAs with CRISPR-GE

CRISPR-Local

CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
AGGGTGTCTCCTTCTCTAAC+TGG	0.224700	4_1:+84674407	None:intergenic
CCTCACTTGGTTGCGGTGGC+CGG	0.305223	4_1:+84675122	None:intergenic
TGCTGTTGTTGTCAAGGTTA+TGG	0.305748	4_1:-84674519	Msa0579830:CDS
CTCACTTGGTTGCGGTGGCC+GGG	0.328965	4_1:+84675123	None:intergenic
GCTGTTGTTGTCAAGGTTAT+GGG	0.344081	4_1:-84674518	Msa0579830:CDS
GAGGACAGGTGACTCAAGTT+AGG	0.354183	4_1:-84674481	Msa0579830:CDS
TTCCTCATGATGTGACGGTT+CGG	0.361521	4_1:+84674440	None:intergenic
CAAAATTCAACCAAATAAAA+AGG	0.363873	4_1:+84674212	None:intergenic
TCCTCATGATGTGACGGTTC+GGG	0.372907	4_1:+84674441	None:intergenic
CTTCTCTTTCAGACTCAAGC+AGG	0.393026	4_1:+84674381	None:intergenic
TCACTTGGTTGCGGTGGCCG+GGG	0.420954	4_1:+84675124	None:intergenic
GAAAGCATAAAGCGAAAAGT+AGG	0.435439	4_1:-84675198	Msa0579830:five_prime_UTR
GTGCAGGATTGGTCTGGTGC+AGG	0.461885	4_1:-84674324	Msa0579830:three_prime_UTR
GCTAGAGAAACTCAGCGGTT+TGG	0.474314	4_1:-84674346	Msa0579830:exon
GAAACTCAGCGGTTTGGTGC+AGG	0.476171	4_1:-84674340	Msa0579830:three_prime_UTR
TCAGCGGTTTGGTGCAGGAT+TGG	0.481241	4_1:-84674335	Msa0579830:three_prime_UTR
GTCAAGGTTATGGGTCGTAC+TGG	0.486004	4_1:-84674509	Msa0579830:CDS
ATTGAGTGTTCTTCTTCCCC+CGG	0.494777	4_1:-84675141	Msa0579830:five_prime_UTR
TTTCAGACTCAAGCAGGGTG+AGG	0.496062	4_1:+84674387	None:intergenic
CACTTGGTTGCGGTGGCCGG+GGG	0.496936	4_1:+84675125	None:intergenic
GGGTGTCTCCTTCTCTAACT+GGG	0.522501	4_1:+84674408	None:intergenic
AGGACAGGTGACTCAAGTTA+GGG	0.535175	4_1:-84674480	Msa0579830:CDS
CAAGTATGCTGTTGTTGTCA+AGG	0.536125	4_1:-84674525	Msa0579830:CDS
GGTTTGGTGCAGGATTGGTC+TGG	0.538529	4_1:-84674330	Msa0579830:three_prime_UTR
TCGTACTGGTTCTAGAGGAC+AGG	0.538891	4_1:-84674495	Msa0579830:CDS
TTCAGACTCAAGCAGGGTGA+GGG	0.547860	4_1:+84674388	None:intergenic
ACCCGAACCGTCACATCATG+AGG	0.555528	4_1:-84674442	Msa0579830:CDS
AAAGCATAAAGCGAAAAGTA+GGG	0.572772	4_1:-84675197	Msa0579830:five_prime_UTR
GAAGAAACCTCACTTGGTTG+CGG	0.577435	4_1:+84675115	None:intergenic
GAAACCTCACTTGGTTGCGG+TGG	0.582952	4_1:+84675118	None:intergenic
TTCTCTTTCAGACTCAAGCA+GGG	0.585301	4_1:+84674382	None:intergenic
TCACATCATGAGGAATGTGA+AGG	0.587194	4_1:-84674432	Msa0579830:CDS
TCACATTCCTCATGATGTGA+CGG	0.589714	4_1:+84674435	None:intergenic
CACATCATGAGGAATGTGAA+GGG	0.593653	4_1:-84674431	Msa0579830:CDS
GTGAAGGGCCCAGTTAGAGA+AGG	0.598823	4_1:-84674416	Msa0579830:CDS
AGTCTGAAAGAGAAGCAAGA+AGG	0.599710	4_1:-84674373	Msa0579830:CDS
ATGGGTCGTACTGGTTCTAG+AGG	0.630812	4_1:-84674500	Msa0579830:CDS
CTCAATCAAGCTCTGCTCTG+CGG	0.700611	4_1:+84675158	None:intergenic
GTTGCGCTAGAGAAACTCAG+CGG	0.778395	4_1:-84674351	Msa0579830:exon

CRISPR-GE

badsite warning	sgRNA_sequence	Strand	Position	Region	GC_content
!!	TTAAAAAAAACAAAGAAAAA+AGG	+	chr4_1:84674331-84674350	None:intergenic	10.0%
!!	TTAAAAAAAACAAAGAAAAA+AGG	+	chr4_1:84674331-84674350	None:intergenic	10.0%
!!!	ATAAAAAACGATTTTCAATT+AGG	+	chr4_1:84674556-84674575	None:intergenic	15.0%
!!!	TTCAATAAACATTTGTTTTT+TGG	-	chr4_1:84675044-84675063	Msa0579830:intron	15.0%
!!!	TCAATAAACATTTGTTTTTT+GGG	-	chr4_1:84675045-84675064	Msa0579830:intron	15.0%
!!!	ATAAAAAACGATTTTCAATT+AGG	+	chr4_1:84674556-84674575	None:intergenic	15.0%
!!!	TTCAATAAACATTTGTTTTT+TGG	-	chr4_1:84675044-84675063	Msa0579830:intron	15.0%
!!!	TCAATAAACATTTGTTTTTT+GGG	-	chr4_1:84675045-84675064	Msa0579830:intron	15.0%
!!	AGAATGAATGAAATTGAATA+AGG	+	chr4_1:84674271-84674290	None:intergenic	20.0%
!!!	AATTTTCGATTCATGATAAT+TGG	+	chr4_1:84674485-84674504	None:intergenic	20.0%
!!	AATTATCATGAATCGAAAAT+TGG	-	chr4_1:84674484-84674503	Msa0579830:CDS	20.0%
!!!	TGATTTCATATTAGTCTAAA+TGG	-	chr4_1:84674609-84674628	Msa0579830:intron	20.0%
!!!	ACATAACAAATCTAAAATCA+TGG	+	chr4_1:84674722-84674741	None:intergenic	20.0%
!!	CATTTACAATGTCAATAAAT+GGG	+	chr4_1:84674767-84674786	None:intergenic	20.0%
!!	ACATTTACAATGTCAATAAA+TGG	+	chr4_1:84674768-84674787	None:intergenic	20.0%
!!!	CAATAAACATTTGTTTTTTG+GGG	-	chr4_1:84675046-84675065	Msa0579830:intron	20.0%
!!	CAAAATTCAACCAAATAAAA+AGG	+	chr4_1:84675135-84675154	None:intergenic	20.0%
!!	AGAATGAATGAAATTGAATA+AGG	+	chr4_1:84674271-84674290	None:intergenic	20.0%
!!!	AATTTTCGATTCATGATAAT+TGG	+	chr4_1:84674485-84674504	None:intergenic	20.0%
!!	AATTATCATGAATCGAAAAT+TGG	-	chr4_1:84674484-84674503	Msa0579830:CDS	20.0%
!!!	TGATTTCATATTAGTCTAAA+TGG	-	chr4_1:84674609-84674628	Msa0579830:intron	20.0%
!!!	ACATAACAAATCTAAAATCA+TGG	+	chr4_1:84674722-84674741	None:intergenic	20.0%
!!	CATTTACAATGTCAATAAAT+GGG	+	chr4_1:84674767-84674786	None:intergenic	20.0%
!!	ACATTTACAATGTCAATAAA+TGG	+	chr4_1:84674768-84674787	None:intergenic	20.0%
!!!	CAATAAACATTTGTTTTTTG+GGG	-	chr4_1:84675046-84675065	Msa0579830:intron	20.0%
!!	CAAAATTCAACCAAATAAAA+AGG	+	chr4_1:84675135-84675154	None:intergenic	20.0%
!	AATATGAAATCAGTTACACA+TGG	+	chr4_1:84674601-84674620	None:intergenic	25.0%
!	AATATGAAATCAGTTACACA+TGG	+	chr4_1:84674601-84674620	None:intergenic	25.0%
	AAAGCATAAAGCGAAAAGTA+GGG	-	chr4_1:84674147-84674166	Msa0579830:three_prime_UTR	30.0%
!	TTTTTCTCTGCTAGATCTAT+TGG	-	chr4_1:84674520-84674539	Msa0579830:CDS	30.0%
	AATGGTATATATCATTCAGC+TGG	+	chr4_1:84674673-84674692	None:intergenic	30.0%
	TAAATGGGAACAATAAGGAA+GGG	+	chr4_1:84674752-84674771	None:intergenic	30.0%
	ATAAATGGGAACAATAAGGA+AGG	+	chr4_1:84674753-84674772	None:intergenic	30.0%
	GTCAATAAATGGGAACAATA+AGG	+	chr4_1:84674757-84674776	None:intergenic	30.0%
	AAAGCATAAAGCGAAAAGTA+GGG	-	chr4_1:84674147-84674166	Msa0579830:three_prime_UTR	30.0%
!	TTTTTCTCTGCTAGATCTAT+TGG	-	chr4_1:84674520-84674539	Msa0579830:CDS	30.0%
	AATGGTATATATCATTCAGC+TGG	+	chr4_1:84674673-84674692	None:intergenic	30.0%
	TAAATGGGAACAATAAGGAA+GGG	+	chr4_1:84674752-84674771	None:intergenic	30.0%
	ATAAATGGGAACAATAAGGA+AGG	+	chr4_1:84674753-84674772	None:intergenic	30.0%
	GTCAATAAATGGGAACAATA+AGG	+	chr4_1:84674757-84674776	None:intergenic	30.0%
!	GAAAGCATAAAGCGAAAAGT+AGG	-	chr4_1:84674146-84674165	Msa0579830:three_prime_UTR	35.0%
	GAAATCAGTTACACATGGTT+GGG	+	chr4_1:84674596-84674615	None:intergenic	35.0%
	TGAAATCAGTTACACATGGT+TGG	+	chr4_1:84674597-84674616	None:intergenic	35.0%
!	CTTAAATTTTGCACCTGTTG+TGG	-	chr4_1:84675104-84675123	Msa0579830:intron	35.0%
!	TGTGGCATGACCTTTTTATT+TGG	-	chr4_1:84675122-84675141	Msa0579830:intron	35.0%
!!!	CTTTGACTATCTGCTGTTTT+TGG	-	chr4_1:84675167-84675186	Msa0579830:five_prime_UTR	35.0%
!!!	CTGTTTTTGGATGTAGTTGT+GGG	-	chr4_1:84675180-84675199	Msa0579830:five_prime_UTR	35.0%
!	GAAAGCATAAAGCGAAAAGT+AGG	-	chr4_1:84674146-84674165	Msa0579830:three_prime_UTR	35.0%
	GAAATCAGTTACACATGGTT+GGG	+	chr4_1:84674596-84674615	None:intergenic	35.0%
	TGAAATCAGTTACACATGGT+TGG	+	chr4_1:84674597-84674616	None:intergenic	35.0%
!	CTTAAATTTTGCACCTGTTG+TGG	-	chr4_1:84675104-84675123	Msa0579830:intron	35.0%
!	TGTGGCATGACCTTTTTATT+TGG	-	chr4_1:84675122-84675141	Msa0579830:intron	35.0%
!!!	CTTTGACTATCTGCTGTTTT+TGG	-	chr4_1:84675167-84675186	Msa0579830:five_prime_UTR	35.0%
!!!	CTGTTTTTGGATGTAGTTGT+GGG	-	chr4_1:84675180-84675199	Msa0579830:five_prime_UTR	35.0%
	GATGAAGAAGAAACCTCACT+TGG	+	chr4_1:84674238-84674257	None:intergenic	40.0%
	GGTATATATCATTCAGCTGG+TGG	+	chr4_1:84674670-84674689	None:intergenic	40.0%
	ACTCTCGAATTTCAGCAGAA+TGG	+	chr4_1:84674691-84674710	None:intergenic	40.0%
	CAAGTATGCTGTTGTTGTCA+AGG	-	chr4_1:84674819-84674838	Msa0579830:intron	40.0%
	TGCTGTTGTTGTCAAGGTTA+TGG	-	chr4_1:84674825-84674844	Msa0579830:intron	40.0%
	GCTGTTGTTGTCAAGGTTAT+GGG	-	chr4_1:84674826-84674845	Msa0579830:intron	40.0%
	TCACATTCCTCATGATGTGA+CGG	+	chr4_1:84674912-84674931	None:intergenic	40.0%
	TCACATCATGAGGAATGTGA+AGG	-	chr4_1:84674912-84674931	Msa0579830:intron	40.0%
	CACATCATGAGGAATGTGAA+GGG	-	chr4_1:84674913-84674932	Msa0579830:intron	40.0%
	TTCTCTTTCAGACTCAAGCA+GGG	+	chr4_1:84674965-84674984	None:intergenic	40.0%
	AGTCTGAAAGAGAAGCAAGA+AGG	-	chr4_1:84674971-84674990	Msa0579830:intron	40.0%
	TAAAAAGGTCATGCCACAAC+AGG	+	chr4_1:84675120-84675139	None:intergenic	40.0%
!!!	GCTGTTTTTGGATGTAGTTG+TGG	-	chr4_1:84675179-84675198	Msa0579830:five_prime_UTR	40.0%
	GATGAAGAAGAAACCTCACT+TGG	+	chr4_1:84674238-84674257	None:intergenic	40.0%
	GGTATATATCATTCAGCTGG+TGG	+	chr4_1:84674670-84674689	None:intergenic	40.0%
	ACTCTCGAATTTCAGCAGAA+TGG	+	chr4_1:84674691-84674710	None:intergenic	40.0%
	CAAGTATGCTGTTGTTGTCA+AGG	-	chr4_1:84674819-84674838	Msa0579830:intron	40.0%
	TGCTGTTGTTGTCAAGGTTA+TGG	-	chr4_1:84674825-84674844	Msa0579830:intron	40.0%
	GCTGTTGTTGTCAAGGTTAT+GGG	-	chr4_1:84674826-84674845	Msa0579830:intron	40.0%
	TCACATTCCTCATGATGTGA+CGG	+	chr4_1:84674912-84674931	None:intergenic	40.0%
	TCACATCATGAGGAATGTGA+AGG	-	chr4_1:84674912-84674931	Msa0579830:intron	40.0%
	CACATCATGAGGAATGTGAA+GGG	-	chr4_1:84674913-84674932	Msa0579830:intron	40.0%
	TTCTCTTTCAGACTCAAGCA+GGG	+	chr4_1:84674965-84674984	None:intergenic	40.0%
	AGTCTGAAAGAGAAGCAAGA+AGG	-	chr4_1:84674971-84674990	Msa0579830:intron	40.0%
	TAAAAAGGTCATGCCACAAC+AGG	+	chr4_1:84675120-84675139	None:intergenic	40.0%
!!!	GCTGTTTTTGGATGTAGTTG+TGG	-	chr4_1:84675179-84675198	Msa0579830:five_prime_UTR	40.0%
	ATTGAGTGTTCTTCTTCCCC+CGG	-	chr4_1:84674203-84674222	Msa0579830:three_prime_UTR	45.0%
	GAAGAAACCTCACTTGGTTG+CGG	+	chr4_1:84674232-84674251	None:intergenic	45.0%
	GTGCTTGTGTTTGAGCAGAA+TGG	-	chr4_1:84674786-84674805	Msa0579830:intron	45.0%
	AGGACAGGTGACTCAAGTTA+GGG	-	chr4_1:84674864-84674883	Msa0579830:intron	45.0%
	TTCCTCATGATGTGACGGTT+CGG	+	chr4_1:84674907-84674926	None:intergenic	45.0%
	CTTCTCTTTCAGACTCAAGC+AGG	+	chr4_1:84674966-84674985	None:intergenic	45.0%
	ATTGAGTGTTCTTCTTCCCC+CGG	-	chr4_1:84674203-84674222	Msa0579830:three_prime_UTR	45.0%
	GAAGAAACCTCACTTGGTTG+CGG	+	chr4_1:84674232-84674251	None:intergenic	45.0%
	GTGCTTGTGTTTGAGCAGAA+TGG	-	chr4_1:84674786-84674805	Msa0579830:intron	45.0%
	AGGACAGGTGACTCAAGTTA+GGG	-	chr4_1:84674864-84674883	Msa0579830:intron	45.0%
	TTCCTCATGATGTGACGGTT+CGG	+	chr4_1:84674907-84674926	None:intergenic	45.0%
	CTTCTCTTTCAGACTCAAGC+AGG	+	chr4_1:84674966-84674985	None:intergenic	45.0%
!!	AATTATACTTAATAAAAATT+CGG	+	chr4_1:84674441-84674460	None:intergenic	5.0%
!!	AATTATACTTAATAAAAATT+CGG	+	chr4_1:84674441-84674460	None:intergenic	5.0%
	CTCAATCAAGCTCTGCTCTG+CGG	+	chr4_1:84674189-84674208	None:intergenic	50.0%
	GTCAAGGTTATGGGTCGTAC+TGG	-	chr4_1:84674835-84674854	Msa0579830:intron	50.0%
!!	ATGGGTCGTACTGGTTCTAG+AGG	-	chr4_1:84674844-84674863	Msa0579830:intron	50.0%
!!	TCGTACTGGTTCTAGAGGAC+AGG	-	chr4_1:84674849-84674868	Msa0579830:intron	50.0%
	GAGGACAGGTGACTCAAGTT+AGG	-	chr4_1:84674863-84674882	Msa0579830:intron	50.0%
	TCCTCATGATGTGACGGTTC+GGG	+	chr4_1:84674906-84674925	None:intergenic	50.0%
	GGGTGTCTCCTTCTCTAACT+GGG	+	chr4_1:84674939-84674958	None:intergenic	50.0%
!	AGGGTGTCTCCTTCTCTAAC+TGG	+	chr4_1:84674940-84674959	None:intergenic	50.0%
	TTCAGACTCAAGCAGGGTGA+GGG	+	chr4_1:84674959-84674978	None:intergenic	50.0%
	TTTCAGACTCAAGCAGGGTG+AGG	+	chr4_1:84674960-84674979	None:intergenic	50.0%
	GTTGCGCTAGAGAAACTCAG+CGG	-	chr4_1:84674993-84675012	Msa0579830:intron	50.0%
	GCTAGAGAAACTCAGCGGTT+TGG	-	chr4_1:84674998-84675017	Msa0579830:intron	50.0%
	CTCAATCAAGCTCTGCTCTG+CGG	+	chr4_1:84674189-84674208	None:intergenic	50.0%
	GTCAAGGTTATGGGTCGTAC+TGG	-	chr4_1:84674835-84674854	Msa0579830:intron	50.0%
!!	ATGGGTCGTACTGGTTCTAG+AGG	-	chr4_1:84674844-84674863	Msa0579830:intron	50.0%
!!	TCGTACTGGTTCTAGAGGAC+AGG	-	chr4_1:84674849-84674868	Msa0579830:intron	50.0%
	GAGGACAGGTGACTCAAGTT+AGG	-	chr4_1:84674863-84674882	Msa0579830:intron	50.0%
	TCCTCATGATGTGACGGTTC+GGG	+	chr4_1:84674906-84674925	None:intergenic	50.0%
	GGGTGTCTCCTTCTCTAACT+GGG	+	chr4_1:84674939-84674958	None:intergenic	50.0%
!	AGGGTGTCTCCTTCTCTAAC+TGG	+	chr4_1:84674940-84674959	None:intergenic	50.0%
	TTCAGACTCAAGCAGGGTGA+GGG	+	chr4_1:84674959-84674978	None:intergenic	50.0%
	TTTCAGACTCAAGCAGGGTG+AGG	+	chr4_1:84674960-84674979	None:intergenic	50.0%
	GTTGCGCTAGAGAAACTCAG+CGG	-	chr4_1:84674993-84675012	Msa0579830:intron	50.0%
	GCTAGAGAAACTCAGCGGTT+TGG	-	chr4_1:84674998-84675017	Msa0579830:intron	50.0%
	GAAACCTCACTTGGTTGCGG+TGG	+	chr4_1:84674229-84674248	None:intergenic	55.0%
	ACCCGAACCGTCACATCATG+AGG	-	chr4_1:84674902-84674921	Msa0579830:intron	55.0%
	GTGAAGGGCCCAGTTAGAGA+AGG	-	chr4_1:84674928-84674947	Msa0579830:intron	55.0%
!!	GAAACTCAGCGGTTTGGTGC+AGG	-	chr4_1:84675004-84675023	Msa0579830:intron	55.0%
!!	TCAGCGGTTTGGTGCAGGAT+TGG	-	chr4_1:84675009-84675028	Msa0579830:intron	55.0%
!!	GGTTTGGTGCAGGATTGGTC+TGG	-	chr4_1:84675014-84675033	Msa0579830:intron	55.0%
	GAAACCTCACTTGGTTGCGG+TGG	+	chr4_1:84674229-84674248	None:intergenic	55.0%
	ACCCGAACCGTCACATCATG+AGG	-	chr4_1:84674902-84674921	Msa0579830:intron	55.0%
	GTGAAGGGCCCAGTTAGAGA+AGG	-	chr4_1:84674928-84674947	Msa0579830:intron	55.0%
!!	GAAACTCAGCGGTTTGGTGC+AGG	-	chr4_1:84675004-84675023	Msa0579830:intron	55.0%
!!	TCAGCGGTTTGGTGCAGGAT+TGG	-	chr4_1:84675009-84675028	Msa0579830:intron	55.0%
!!	GGTTTGGTGCAGGATTGGTC+TGG	-	chr4_1:84675014-84675033	Msa0579830:intron	55.0%
!!	GTGCAGGATTGGTCTGGTGC+AGG	-	chr4_1:84675020-84675039	Msa0579830:intron	60.0%
!!	GTGCAGGATTGGTCTGGTGC+AGG	-	chr4_1:84675020-84675039	Msa0579830:intron	60.0%
	TCACTTGGTTGCGGTGGCCG+GGG	+	chr4_1:84674223-84674242	None:intergenic	65.0%
	CTCACTTGGTTGCGGTGGCC+GGG	+	chr4_1:84674224-84674243	None:intergenic	65.0%
	CCTCACTTGGTTGCGGTGGC+CGG	+	chr4_1:84674225-84674244	None:intergenic	65.0%
	TCACTTGGTTGCGGTGGCCG+GGG	+	chr4_1:84674223-84674242	None:intergenic	65.0%
	CTCACTTGGTTGCGGTGGCC+GGG	+	chr4_1:84674224-84674243	None:intergenic	65.0%
	CCTCACTTGGTTGCGGTGGC+CGG	+	chr4_1:84674225-84674244	None:intergenic	65.0%
	CACTTGGTTGCGGTGGCCGG+GGG	+	chr4_1:84674222-84674241	None:intergenic	70.0%
	CCGGCCACCGCAACCAAGTG+AGG	-	chr4_1:84674222-84674241	Msa0579830:three_prime_UTR	70.0%
	CACTTGGTTGCGGTGGCCGG+GGG	+	chr4_1:84674222-84674241	None:intergenic	70.0%
	CCGGCCACCGCAACCAAGTG+AGG	-	chr4_1:84674222-84674241	Msa0579830:three_prime_UTR	70.0%

Chromosome	Type	Strat	End	Strand	Name
chr4_1	gene	84674125	84675241	84674125	ID=Msa0579830;Name=Msa0579830
chr4_1	mRNA	84674125	84675241	84674125	ID=Msa0579830-mRNA-1;Parent=Msa0579830;Name=Msa0579830-mRNA-1;_AED=0.00;_eAED=0.00;_QI=114\|0\|0.5\|1\|0\|0.5\|2\|239\|65
chr4_1	exon	84674125	84674561	84674125	ID=Msa0579830-mRNA-1:exon:31024;Parent=Msa0579830-mRNA-1
chr4_1	exon	84675128	84675241	84675128	ID=Msa0579830-mRNA-1:exon:31023;Parent=Msa0579830-mRNA-1
chr4_1	five_prime_UTR	84675128	84675241	84675128	ID=Msa0579830-mRNA-1:five_prime_utr;Parent=Msa0579830-mRNA-1
chr4_1	CDS	84674364	84674561	84674364	ID=Msa0579830-mRNA-1:cds;Parent=Msa0579830-mRNA-1
chr4_1	three_prime_UTR	84674125	84674363	84674125	ID=Msa0579830-mRNA-1:three_prime_utr;Parent=Msa0579830-mRNA-1

Gene Sequence	>Msa0579830 ATGGATTCTCAAGTCAAGTATGCTGTTGTTGTCAAGGTTATGGGTCGTACTGGTTCTAGAGGACAGGTGACTCAAGTTAGGGTCAAGTTTAATGACGACCCGAACCGTCACATCATGAGGAATGTGAAGGGCCCAGTTAGAGAAGGAGACACCCTCACCCTGCTTGAGTCTGAAAGAGAAGCAAGAAGGTTGCGCTAG
Protein sequence	>Msa0579830 MDSQVKYAVVVKVMGRTGSRGQVTQVRVKFNDDPNRHIMRNVKGPVREGDTLTLLESEREARRLR*